DE19530132C2 - Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien - Google Patents

Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien

Info

Publication number
DE19530132C2
DE19530132C2 DE19530132A DE19530132A DE19530132C2 DE 19530132 C2 DE19530132 C2 DE 19530132C2 DE 19530132 A DE19530132 A DE 19530132A DE 19530132 A DE19530132 A DE 19530132A DE 19530132 C2 DE19530132 C2 DE 19530132C2
Authority
DE
Germany
Prior art keywords
nucleic acids
adsorption matrix
reagent kit
sample
isolation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE19530132A
Other languages
German (de)
English (en)
Other versions
DE19530132A1 (de
Inventor
Oliver Mueller
Rainer Dipl Biol Deuter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DE19530132A priority Critical patent/DE19530132C2/de
Application filed by Max Planck Gesellschaft zur Foerderung der Wissenschaften eV filed Critical Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority to DE59609027T priority patent/DE59609027D1/de
Priority to EP96928466A priority patent/EP0851937B1/de
Priority to JP50894597A priority patent/JP4264133B2/ja
Priority to DK96928466T priority patent/DK0851937T3/da
Priority to AT96928466T priority patent/ATE215611T1/de
Priority to US09/011,567 priority patent/US6084091A/en
Priority to AU68216/96A priority patent/AU712331B2/en
Priority to PCT/EP1996/003595 priority patent/WO1997007239A1/de
Priority to CA2228769A priority patent/CA2228769C/en
Publication of DE19530132A1 publication Critical patent/DE19530132A1/de
Application granted granted Critical
Publication of DE19530132C2 publication Critical patent/DE19530132C2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Processing Of Solid Wastes (AREA)
  • Detergent Compositions (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
DE19530132A 1995-08-16 1995-08-16 Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien Expired - Lifetime DE19530132C2 (de)

Priority Applications (10)

Application Number Priority Date Filing Date Title
DE19530132A DE19530132C2 (de) 1995-08-16 1995-08-16 Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
PCT/EP1996/003595 WO1997007239A1 (de) 1995-08-16 1996-08-14 Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien
JP50894597A JP4264133B2 (ja) 1995-08-16 1996-08-14 生物学的物質からの核酸を精製、安定化又は単離する方法
DK96928466T DK0851937T3 (da) 1995-08-16 1996-08-14 Fremgangsmåde til oprensning, stabilisering eller isolering af nucleinsyrer fra biologiske materialer
AT96928466T ATE215611T1 (de) 1995-08-16 1996-08-14 Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien
US09/011,567 US6084091A (en) 1995-08-16 1996-08-14 Process for purifying, stabilising or isolating nucleic acids from biological materials
DE59609027T DE59609027D1 (de) 1995-08-16 1996-08-14 Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien
EP96928466A EP0851937B1 (de) 1995-08-16 1996-08-14 Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien
CA2228769A CA2228769C (en) 1995-08-16 1996-08-14 Method of purifying, stabilising or isolating nucleic acids contained in biological matter
AU68216/96A AU712331B2 (en) 1995-08-16 1996-08-14 Process for purifying, stabilising or isolating nucleic acids contained in biological materials

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19530132A DE19530132C2 (de) 1995-08-16 1995-08-16 Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien

Publications (2)

Publication Number Publication Date
DE19530132A1 DE19530132A1 (de) 1997-02-20
DE19530132C2 true DE19530132C2 (de) 1998-07-16

Family

ID=7769624

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19530132A Expired - Lifetime DE19530132C2 (de) 1995-08-16 1995-08-16 Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
DE59609027T Expired - Lifetime DE59609027D1 (de) 1995-08-16 1996-08-14 Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE59609027T Expired - Lifetime DE59609027D1 (de) 1995-08-16 1996-08-14 Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien

Country Status (9)

Country Link
US (1) US6084091A (https=)
EP (1) EP0851937B1 (https=)
JP (1) JP4264133B2 (https=)
AT (1) ATE215611T1 (https=)
AU (1) AU712331B2 (https=)
CA (1) CA2228769C (https=)
DE (2) DE19530132C2 (https=)
DK (1) DK0851937T3 (https=)
WO (1) WO1997007239A1 (https=)

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US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
US6964846B1 (en) 1999-04-09 2005-11-15 Exact Sciences Corporation Methods for detecting nucleic acids indicative of cancer
US7368233B2 (en) 1999-12-07 2008-05-06 Exact Sciences Corporation Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm
US7811757B2 (en) 1999-09-08 2010-10-12 Genzyme Corporation Methods for disease detection

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AU7816898A (en) * 1997-06-16 1999-01-04 Exact Laboratories, Inc. Methods for stool sample preparation
US6406857B1 (en) 1997-06-16 2002-06-18 Exact Sciences Corporation Methods for stool sample preparation
US6268136B1 (en) 1997-06-16 2001-07-31 Exact Science Corporation Methods for stool sample preparation
US6241980B1 (en) * 1997-11-04 2001-06-05 Becton, Dickinson And Company Sample processing method using ion exchange resin
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
EP0939118A1 (en) * 1998-02-20 1999-09-01 Universiteit Maastricht Method for isolating DNA and RNA from faeces
ES2339725T3 (es) 1998-04-08 2010-05-24 Metabolix, Inc. Metodos para la separacion y purificacion de biopolimeros.
ATE336537T1 (de) 1998-06-09 2006-09-15 Metabolix Inc Verfahren und vorrichtung zur herstellung von amorphen polymersuspensionen
DE19900638C2 (de) * 1999-01-11 2002-12-19 Max Planck Gesellschaft Methode zur Isolierung von DNA aus biologischen Materialien
AU752817B2 (en) * 1999-02-25 2002-10-03 Exact Sciences Corporation Methods for preserving DNA integrity
US6383783B1 (en) 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
US7005266B2 (en) * 2000-02-04 2006-02-28 Qiagen Gmbh Nucleic acid isolation from stool samples and other inhibitor-rich biological materials
AU2001238616A1 (en) * 2000-02-23 2001-09-03 Wen Shao Rapid nucleic acid separation, isolation and purification methods
GB0015777D0 (en) * 2000-06-28 2000-08-16 Tayside University Hospitals N DNA Extraction
EP1442045B1 (en) * 2001-11-06 2009-01-07 Cortex Biochem Inc. Isolation and purification of nucleic acids
GB0130821D0 (en) * 2001-12-24 2002-02-06 Tayside University Hospitals N DNA screening
US7776524B2 (en) 2002-02-15 2010-08-17 Genzyme Corporation Methods for analysis of molecular events
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
US20040259101A1 (en) * 2003-06-20 2004-12-23 Shuber Anthony P. Methods for disease screening
WO2005026347A1 (en) * 2003-09-09 2005-03-24 Fuji Photo Film Co., Ltd. Method for isolating and purifying a nucleic acid
BRPI0415046A (pt) * 2003-10-02 2006-12-12 Univ Mississippi produção de biodiesel e outras substáncias quìmicas valiosas a partir de resìduos de plantas de tratamento de água residual
US20050130177A1 (en) * 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US20050142571A1 (en) * 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using solid phase material
US7727710B2 (en) * 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
KR20060129340A (ko) * 2003-12-30 2006-12-15 시그마-알드리치컴퍼니 효소 소화에 의한 핵산의 신속한 제조
US8426126B2 (en) 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
WO2005111244A2 (en) * 2004-05-10 2005-11-24 Exact Sciences Corporation Methods for detecting a mutant nucleic acid
WO2006026654A2 (en) 2004-08-27 2006-03-09 Exact Sciences Corporation Method for detecting a recombinant event
US7195878B2 (en) * 2004-09-30 2007-03-27 Cryo-Genomics Device and method for fecal testing
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US9777314B2 (en) * 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
JP4819427B2 (ja) 2005-07-15 2011-11-24 株式会社リコー 画像形成装置、画像形成方法、及びプロセスカートリッジ
JP4590344B2 (ja) 2005-11-21 2010-12-01 株式会社リコー 静電潜像担持体及びそれを用いた画像形成装置、プロセスカートリッジ及び画像形成方法
JP2009011247A (ja) * 2007-07-05 2009-01-22 Sumitomo Bakelite Co Ltd 遺伝子の検出方法
US7759112B2 (en) * 2007-10-31 2010-07-20 Akonni Biosystems, Inc. Apparatus, system, and method for purifying nucleic acids
US10125388B2 (en) 2007-10-31 2018-11-13 Akonni Biosystems, Inc. Integrated sample processing system
US9428746B2 (en) 2007-10-31 2016-08-30 Akonni Biosystems, Inc. Method and kit for purifying nucleic acids
US20090111193A1 (en) * 2007-10-31 2009-04-30 Cooney Christopher G Sample preparation device
US9347055B2 (en) 2007-11-05 2016-05-24 Eiken Kagaku Kabushiki Kaisha Method and kit for preparation of sample for use in nucleic acid amplification
JP5599013B2 (ja) * 2008-04-03 2014-10-01 キヤノン株式会社 血液検体からの微生物核酸の抽出方法
CN102105583A (zh) * 2008-07-23 2011-06-22 奥林巴斯株式会社 从粪便试样中回收核酸的方法、核酸分析方法以及粪便试样处理装置
WO2010028382A2 (en) * 2008-09-05 2010-03-11 Mayo Foundation For Medical Education And Research Collecting and processing complex macromolecular mixtures
JPWO2010134245A1 (ja) * 2009-05-20 2012-11-08 オリンパス株式会社 哺乳細胞由来核酸の回収方法、核酸解析方法、及び採便用キット
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
LT2521780T (lt) 2010-01-07 2018-02-12 Bigtec Private Limited Neklerūgščių išskyrimo būdas ir rinkinys
JP5743701B2 (ja) * 2011-05-10 2015-07-01 英二 小西 Rna検出用試薬およびrna検出方法
JP5743704B2 (ja) * 2011-05-12 2015-07-01 住友大阪セメント株式会社 Rna検出用試薬およびrna検出方法
WO2012177656A2 (en) 2011-06-19 2012-12-27 Abogen, Inc. Devices, solutions and methods for sample collection
CN103890176B (zh) * 2011-09-06 2016-10-26 西北大学 制备生物材料的方法
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6964846B1 (en) 1999-04-09 2005-11-15 Exact Sciences Corporation Methods for detecting nucleic acids indicative of cancer
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US7811757B2 (en) 1999-09-08 2010-10-12 Genzyme Corporation Methods for disease detection
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
US7368233B2 (en) 1999-12-07 2008-05-06 Exact Sciences Corporation Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm
US7981612B2 (en) 1999-12-07 2011-07-19 Mayo Foundation For Medical Education And Research Methods of screening for supracolonic neoplasms based on stool samples containing a nucleic acid marker indicative of a neoplasm

Also Published As

Publication number Publication date
CA2228769A1 (en) 1997-02-27
ATE215611T1 (de) 2002-04-15
US6084091A (en) 2000-07-04
JPH11511020A (ja) 1999-09-28
JP4264133B2 (ja) 2009-05-13
DE19530132A1 (de) 1997-02-20
EP0851937A1 (de) 1998-07-08
DE59609027D1 (de) 2002-05-08
CA2228769C (en) 2011-04-12
DK0851937T3 (da) 2002-06-24
AU6821696A (en) 1997-03-12
EP0851937B1 (de) 2002-04-03
WO1997007239A1 (de) 1997-02-27
AU712331B2 (en) 1999-11-04

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