DE1620645A1 - Verfahren zur in-vitro-Synthese biologisch aktiver intakter Nucleinsaeure und Herstellung von Replicasen,die hierfuer geeignet sind - Google Patents

Verfahren zur in-vitro-Synthese biologisch aktiver intakter Nucleinsaeure und Herstellung von Replicasen,die hierfuer geeignet sind

Info

Publication number
DE1620645A1
DE1620645A1 DE19661620645 DE1620645A DE1620645A1 DE 1620645 A1 DE1620645 A1 DE 1620645A1 DE 19661620645 DE19661620645 DE 19661620645 DE 1620645 A DE1620645 A DE 1620645A DE 1620645 A1 DE1620645 A1 DE 1620645A1
Authority
DE
Germany
Prior art keywords
rna
virus
replicase
acid
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE19661620645
Other languages
German (de)
English (en)
Inventor
Ichiro Haruna
Pace Norman R
Dr Solomon Spiegelman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Illinois at Urbana-Champaign
Original Assignee
University of Illinois at Urbana-Champaign
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Illinois at Urbana-Champaign filed Critical University of Illinois at Urbana-Champaign
Publication of DE1620645A1 publication Critical patent/DE1620645A1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/127—RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DE19661620645 1965-09-29 1966-09-27 Verfahren zur in-vitro-Synthese biologisch aktiver intakter Nucleinsaeure und Herstellung von Replicasen,die hierfuer geeignet sind Pending DE1620645A1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US50945865A 1965-09-29 1965-09-29
US53559666A 1966-03-18 1966-03-18
US55993366A 1966-06-23 1966-06-23

Publications (1)

Publication Number Publication Date
DE1620645A1 true DE1620645A1 (de) 1970-02-12

Family

ID=27414411

Family Applications (3)

Application Number Title Priority Date Filing Date
DE19661620645 Pending DE1620645A1 (de) 1965-09-29 1966-09-27 Verfahren zur in-vitro-Synthese biologisch aktiver intakter Nucleinsaeure und Herstellung von Replicasen,die hierfuer geeignet sind
DE19661717206 Pending DE1717206A1 (de) 1965-09-29 1966-09-27 Verfahren zur in-vitro-Synthese biologisch aktiver intakter Nucleinsaeure und Herstellung von Replicasen,die hierfuer geeignet sind
DE19661695956 Pending DE1695956A1 (de) 1965-09-29 1966-09-27 Verfahren zur in-vitro-Synthese biologisch aktiver intakter Nucleinsaeure und Herstellung von Replicasen,die hierfuer geeignet sind

Family Applications After (2)

Application Number Title Priority Date Filing Date
DE19661717206 Pending DE1717206A1 (de) 1965-09-29 1966-09-27 Verfahren zur in-vitro-Synthese biologisch aktiver intakter Nucleinsaeure und Herstellung von Replicasen,die hierfuer geeignet sind
DE19661695956 Pending DE1695956A1 (de) 1965-09-29 1966-09-27 Verfahren zur in-vitro-Synthese biologisch aktiver intakter Nucleinsaeure und Herstellung von Replicasen,die hierfuer geeignet sind

Country Status (5)

Country Link
US (2) US3444041A (enExample)
BE (1) BE687586A (enExample)
DE (3) DE1620645A1 (enExample)
GB (1) GB1137170A (enExample)
NL (1) NL6613803A (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3907778A (en) * 1969-05-26 1975-09-23 Nakao Ishida Double stranded RNA from basidiomycetes
BE754934A (fr) * 1969-08-16 1971-02-17 Jachertz Diether Procede de production enzymatique d'arn-messager
US4786600A (en) * 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
US4957858A (en) * 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
US4942130A (en) * 1987-01-14 1990-07-17 President & Fellows Of Harvard College T7 DNA polymerase
US4795699A (en) * 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
US5145776A (en) * 1987-01-14 1992-09-08 President & Fellows Of Harvard College Method of using T7 DNA polymerase to mutagenize and fill-in DNA
US4994372A (en) * 1987-01-14 1991-02-19 President And Fellows Of Harvard College DNA sequencing
US5266466A (en) * 1987-01-14 1993-11-30 President And Fellows Of Harvard College Method of using T7 DNA polymerase to label the 3' end of a DNA molecule
US4946786A (en) * 1987-01-14 1990-08-07 President And Fellows Of Harvard College T7 DNA polymerase
WO2003024980A1 (fr) * 2001-09-14 2003-03-27 Universite De Liege Preparation de composes organiques triphosphoryles eventuellement marques au phosphore 32 ou au phosphore 33
CN115044577B (zh) * 2022-07-29 2025-03-18 北京工商大学 一种固定化米根霉脂肪酶及其可控化制备方法和应用

Also Published As

Publication number Publication date
DE1695956A1 (de) 1971-05-19
DE1717206A1 (de) 1971-02-18
GB1137170A (en) 1968-12-18
NL6613803A (enExample) 1967-03-30
US3444041A (en) 1969-05-13
US3444042A (en) 1969-05-13
BE687586A (enExample) 1967-03-29

Similar Documents

Publication Publication Date Title
DE2915082C3 (de) Verfahren und Verwendung eines Reagenzsatzes zum Nachweis und zur Charakterisierung von Nukleinsäuren und ihren Sequenzen
DE2535554C2 (de) Biologisches Verfahren
DE69333242T2 (de) Verfahren, reagenz und salz zum nachweis und amplifikation von nukleinsäuresequenzen
DE3588239T3 (de) Verfahren zum Erhalten von DNS, RNS, Peptiden, Polypeptiden oder Proteinen durch DMS-Rekombinant-Verfahren
DE69512003T2 (de) Oligonukleotid verwendbar als Primer in einem Amplifikationsverfahren auf der Basis einer Strangverdrängungsreplikation
Ottolenghi et al. Release of genetic transforming agent from pneumococcal cultures during growth and disintegration
DE3587299T2 (de) Nachweis und/oder identifizierung von mikroorganismen in einer probe mittels biolumineszenz oder anderer exogenen genetisch eingefuehrten markierungssubstanzen.
DE102007058516B4 (de) Identifizierung von Erregern in Körperflüssigkeiten
DE69133574T2 (de) Verfahren zur Unterscheidung von Sequenz Varianten
DE68929385T2 (de) Verfahren zur Bestimmung eines Analyten unter Verwendung einer replizierbare RNS enthaltenden Sonde
DE3689954T2 (de) Amplifiziertes hybridisierungstestverfahren.
DE69419244T2 (de) Zusammensetzungen und methoden zur anwendung von reaktiven antiviralen polymeren
DE69031237T2 (de) Verfahren zur Reinigung von Nukleinsäuren
DE3751513T2 (de) Nachweis von Viren durch Verstärkung und Hybridisation.
DE69011722T2 (de) Diagnoseausrüstung, Primerzusammensetzung und ihre Verwendung zur Erwiderung oder zum Nachweis von Nukleinsäuren.
DE69622347T2 (de) Oligonukleotid-Primer für Amplifizierung von HCV-Nukleinsäure
EP0851937A1 (de) Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien
DD284692A5 (de) Verfahren zur herstellung prozessiver dna-polymerase
DE69819124T2 (de) Lyophilisation von kultivierten, menschlichen zellen zur konservierung der rna und dna
DE1717206A1 (de) Verfahren zur in-vitro-Synthese biologisch aktiver intakter Nucleinsaeure und Herstellung von Replicasen,die hierfuer geeignet sind
EP2446054B1 (de) Verfahren zur amplifikation von dna unter verwendung von tetraethylenglykol
DE68918457T2 (de) Verfahren zur polymeraseaktivitätsbestimmung.
CH645025A5 (de) Verfahren zur herstellung eines hypotoxinogenen und genetisch bestaendigen abweichenden stammes von vibrio cholerae.
EP0623176B1 (de) Verfahren zum nachweis reverser transcriptase
EP2163239A1 (de) Produkte, die Biopartikel enthalten, Verfahren zu ihrer Herstellung