DE112020002516B4 - Multivalente bindungszusammensetzung zur nukleinsäureanalyse - Google Patents

Multivalente bindungszusammensetzung zur nukleinsäureanalyse Download PDF

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Publication number
DE112020002516B4
DE112020002516B4 DE112020002516.0T DE112020002516T DE112020002516B4 DE 112020002516 B4 DE112020002516 B4 DE 112020002516B4 DE 112020002516 T DE112020002516 T DE 112020002516T DE 112020002516 B4 DE112020002516 B4 DE 112020002516B4
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nucleotide
nucleic acid
polymer
binding
target nucleic
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German (de)
English (en)
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DE112020002516T5 (de
Inventor
Sinan ARSLAN
Molly He
Matthew Kellinger
Jake Levieux
Michael Previte
Junhua Zhao
Su Zhang
Tyler Lopez
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Element Biosciences Inc
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Element Biosciences Inc
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Priority claimed from US16/579,794 external-priority patent/US10768173B1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/157—A reaction step characterised by the number of molecules incorporated or released
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE112020002516.0T 2019-05-24 2020-05-22 Multivalente bindungszusammensetzung zur nukleinsäureanalyse Active DE112020002516B4 (de)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201962852876P 2019-05-24 2019-05-24
US62/852,876 2019-05-24
US201962897172P 2019-09-06 2019-09-06
US62/897,172 2019-09-06
US16/579,794 2019-09-23
US16/579,794 US10768173B1 (en) 2019-09-06 2019-09-23 Multivalent binding composition for nucleic acid analysis
PCT/US2020/034409 WO2020243017A1 (en) 2019-05-24 2020-05-22 Multivalent binding composition for nucleic acid analysis

Publications (2)

Publication Number Publication Date
DE112020002516T5 DE112020002516T5 (de) 2022-03-24
DE112020002516B4 true DE112020002516B4 (de) 2025-03-13

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DE112020002516.0T Active DE112020002516B4 (de) 2019-05-24 2020-05-22 Multivalente bindungszusammensetzung zur nukleinsäureanalyse

Country Status (11)

Country Link
EP (1) EP3947731A4 (https=)
JP (2) JP7542000B2 (https=)
KR (3) KR102823471B1 (https=)
CN (1) CN113939601A (https=)
AU (3) AU2020285657B2 (https=)
CA (1) CA3137120A1 (https=)
DE (1) DE112020002516B4 (https=)
GB (1) GB2597398B (https=)
IL (3) IL316901A (https=)
SG (1) SG11202112049VA (https=)
WO (1) WO2020243017A1 (https=)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019241305A1 (en) 2018-06-12 2019-12-19 Element Biosciences, Inc. Improved reverse transcriptase for nucleic acid sequencing
US10768173B1 (en) 2019-09-06 2020-09-08 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
WO2020102766A2 (en) 2018-11-15 2020-05-22 Element Biosciences, Inc. Methods for generating circular nucleic acid molecules
AU2019392932B2 (en) 2018-12-07 2023-11-02 Element Biosciences, Inc. Flow cell device and use thereof
US12313627B2 (en) 2019-05-01 2025-05-27 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US12391929B2 (en) 2019-05-24 2025-08-19 Element Biosciences, Inc. Polymerase-nucleotide conjugates for sequencing by trapping
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US11060138B1 (en) 2020-01-17 2021-07-13 Element Biosciences, Inc. Nucleic acid sequencing systems
US11198121B1 (en) 2020-06-10 2021-12-14 Element Biosciences, Inc. Flow cell systems and devices
GB2599729A (en) * 2020-10-12 2022-04-13 Sumitomo Chemical Co Method comprising light emitting marker
CA3196800A1 (en) 2020-10-30 2022-05-05 Element Biosciences, Inc. Reagents for massively parallel nucleic acid sequencing
AU2022316142A1 (en) 2021-07-21 2024-02-22 Element Biosciences, Inc. Optical systems for nucleic acid sequencing and methods thereof
KR20230153706A (ko) 2022-04-29 2023-11-07 연세대학교 산학협력단 핵산의 검출 또는 분리용 조성물 및 이를 이용한 핵산의 검출 또는 분리 방법
EP4573213A1 (en) 2022-08-15 2025-06-25 Element Biosciences, Inc. Spatially resolved surface capture of nucleic acids
CN116024315B (zh) * 2022-11-30 2025-09-09 温州医科大学 一种基于链置换反应的多价双锁钥系统及其在核苷酸多态性检测中的应用
WO2026080904A2 (en) 2024-10-11 2026-04-16 Element Biosciences, Inc. Systems and methods for performing dna sequencing

Citations (3)

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WO2005111240A2 (en) 2004-04-30 2005-11-24 Li-Cor, Inc. Field-switch sequencing
WO2009073201A2 (en) 2007-12-04 2009-06-11 Pacific Biosciences Of California, Inc. Alternate labeling strategies for single molecule sequencing
US20090186343A1 (en) 2003-01-28 2009-07-23 Visigen Biotechnologies, Inc. Methods for preparing modified biomolecules, modified biomolecules and methods for using same

Family Cites Families (7)

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Publication number Priority date Publication date Assignee Title
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US9035035B2 (en) * 2004-11-05 2015-05-19 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
US9777320B2 (en) * 2010-08-25 2017-10-03 Pacific Biosciences Of California, Inc. Molecular adaptors for dye conjugates
WO2012150035A1 (de) * 2011-05-04 2012-11-08 Genovoxx Gmbh Nukleosid-triphosphat-konjugate und methoden zu deren anwendung
US9062091B2 (en) * 2012-02-15 2015-06-23 Pacific Biosciences Of California, Inc. Polymerase enzyme substrates with protein shield
EP3589752B1 (en) 2017-01-26 2021-05-19 QIAGEN GmbH Method for generating a sequencing library
WO2018144854A1 (en) 2017-02-02 2018-08-09 Caris Science, Inc. Targeted oligonucleotides

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
US20090186343A1 (en) 2003-01-28 2009-07-23 Visigen Biotechnologies, Inc. Methods for preparing modified biomolecules, modified biomolecules and methods for using same
WO2005111240A2 (en) 2004-04-30 2005-11-24 Li-Cor, Inc. Field-switch sequencing
WO2009073201A2 (en) 2007-12-04 2009-06-11 Pacific Biosciences Of California, Inc. Alternate labeling strategies for single molecule sequencing

Non-Patent Citations (5)

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Title
Haugland, Molecular Probes Handbook, (Eugene, Oreg.), 6. Auflage
Hermanson, Bioconjugate Techniques, 2. Auflage
Lakowicz, Principles of Fluorescence Spectroscopy, 2. Auflage, Plenum Press New York (1999
Lorenz, T.C. J. Vis. Exp. (63), e3998, doi: 10.3791/3998 (2012
Remington: The Science and Practice of Pharmacy, 20. Ausgabe, Gennaro, A.R.: Hrsg. (2000

Also Published As

Publication number Publication date
GB2597398B (en) 2024-03-06
KR20250096873A (ko) 2025-06-27
JP7542000B2 (ja) 2024-08-29
AU2022291540B2 (en) 2024-11-14
IL301380B1 (en) 2025-01-01
IL287528B1 (en) 2023-04-01
IL316901A (en) 2025-01-01
SG11202112049VA (en) 2021-12-30
IL301380A (en) 2023-05-01
DE112020002516T5 (de) 2022-03-24
CA3137120A1 (en) 2020-12-03
EP3947731A4 (en) 2022-06-29
CN113939601A (zh) 2022-01-14
JP2022535187A (ja) 2022-08-05
IL301380B2 (en) 2025-05-01
IL287528B2 (en) 2023-08-01
IL287528A (en) 2021-12-01
EP3947731A1 (en) 2022-02-09
KR20230165871A (ko) 2023-12-05
AU2025200380A1 (en) 2025-02-06
GB202115667D0 (en) 2021-12-15
KR102823471B1 (ko) 2025-06-19
KR20210144929A (ko) 2021-11-30
AU2020285657A1 (en) 2021-11-18
WO2020243017A1 (en) 2020-12-03
KR102607124B1 (ko) 2023-11-29
AU2020285657B2 (en) 2022-10-06
AU2022291540A1 (en) 2023-02-02
JP2024174880A (ja) 2024-12-17
GB2597398A (en) 2022-01-26

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