EP3947731A4 - Multivalent binding composition for nucleic acid analysis - Google Patents

Multivalent binding composition for nucleic acid analysis Download PDF

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Publication number
EP3947731A4
EP3947731A4 EP20815612.5A EP20815612A EP3947731A4 EP 3947731 A4 EP3947731 A4 EP 3947731A4 EP 20815612 A EP20815612 A EP 20815612A EP 3947731 A4 EP3947731 A4 EP 3947731A4
Authority
EP
European Patent Office
Prior art keywords
nucleic acid
acid analysis
binding composition
multivalent binding
multivalent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20815612.5A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP3947731A1 (en
Inventor
Sinan ARSLAN
Molly He
Matthew KELLINGER
Jake Levieux
Michael Previte
Junhua Zhao
Su Zhang
Tyler LOPEZ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Element Biosciences Inc
Original Assignee
Element Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US16/579,794 external-priority patent/US10768173B1/en
Application filed by Element Biosciences Inc filed Critical Element Biosciences Inc
Publication of EP3947731A1 publication Critical patent/EP3947731A1/en
Publication of EP3947731A4 publication Critical patent/EP3947731A4/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/101DNA polymerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/157A reaction step characterised by the number of molecules incorporated or released
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149Particles, e.g. beads

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP20815612.5A 2019-05-24 2020-05-22 Multivalent binding composition for nucleic acid analysis Pending EP3947731A4 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201962852876P 2019-05-24 2019-05-24
US201962897172P 2019-09-06 2019-09-06
US16/579,794 US10768173B1 (en) 2019-09-06 2019-09-23 Multivalent binding composition for nucleic acid analysis
PCT/US2020/034409 WO2020243017A1 (en) 2019-05-24 2020-05-22 Multivalent binding composition for nucleic acid analysis

Publications (2)

Publication Number Publication Date
EP3947731A1 EP3947731A1 (en) 2022-02-09
EP3947731A4 true EP3947731A4 (en) 2022-06-29

Family

ID=73554179

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20815612.5A Pending EP3947731A4 (en) 2019-05-24 2020-05-22 Multivalent binding composition for nucleic acid analysis

Country Status (11)

Country Link
EP (1) EP3947731A4 (https=)
JP (2) JP7542000B2 (https=)
KR (3) KR102823471B1 (https=)
CN (1) CN113939601A (https=)
AU (3) AU2020285657B2 (https=)
CA (1) CA3137120A1 (https=)
DE (1) DE112020002516B4 (https=)
GB (1) GB2597398B (https=)
IL (3) IL316901A (https=)
SG (1) SG11202112049VA (https=)
WO (1) WO2020243017A1 (https=)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019241305A1 (en) 2018-06-12 2019-12-19 Element Biosciences, Inc. Improved reverse transcriptase for nucleic acid sequencing
US10768173B1 (en) 2019-09-06 2020-09-08 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
WO2020102766A2 (en) 2018-11-15 2020-05-22 Element Biosciences, Inc. Methods for generating circular nucleic acid molecules
AU2019392932B2 (en) 2018-12-07 2023-11-02 Element Biosciences, Inc. Flow cell device and use thereof
US12313627B2 (en) 2019-05-01 2025-05-27 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US12391929B2 (en) 2019-05-24 2025-08-19 Element Biosciences, Inc. Polymerase-nucleotide conjugates for sequencing by trapping
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US11060138B1 (en) 2020-01-17 2021-07-13 Element Biosciences, Inc. Nucleic acid sequencing systems
US11198121B1 (en) 2020-06-10 2021-12-14 Element Biosciences, Inc. Flow cell systems and devices
GB2599729A (en) * 2020-10-12 2022-04-13 Sumitomo Chemical Co Method comprising light emitting marker
CA3196800A1 (en) 2020-10-30 2022-05-05 Element Biosciences, Inc. Reagents for massively parallel nucleic acid sequencing
AU2022316142A1 (en) 2021-07-21 2024-02-22 Element Biosciences, Inc. Optical systems for nucleic acid sequencing and methods thereof
KR20230153706A (ko) 2022-04-29 2023-11-07 연세대학교 산학협력단 핵산의 검출 또는 분리용 조성물 및 이를 이용한 핵산의 검출 또는 분리 방법
EP4573213A1 (en) 2022-08-15 2025-06-25 Element Biosciences, Inc. Spatially resolved surface capture of nucleic acids
CN116024315B (zh) * 2022-11-30 2025-09-09 温州医科大学 一种基于链置换反应的多价双锁钥系统及其在核苷酸多态性检测中的应用
WO2026080904A2 (en) 2024-10-11 2026-04-16 Element Biosciences, Inc. Systems and methods for performing dna sequencing

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005111240A2 (en) * 2004-04-30 2005-11-24 Li-Cor, Inc. Field-switch sequencing
WO2009073201A2 (en) * 2007-12-04 2009-06-11 Pacific Biosciences Of California, Inc. Alternate labeling strategies for single molecule sequencing
US20090186343A1 (en) * 2003-01-28 2009-07-23 Visigen Biotechnologies, Inc. Methods for preparing modified biomolecules, modified biomolecules and methods for using same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US9035035B2 (en) * 2004-11-05 2015-05-19 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
US9777320B2 (en) * 2010-08-25 2017-10-03 Pacific Biosciences Of California, Inc. Molecular adaptors for dye conjugates
WO2012150035A1 (de) * 2011-05-04 2012-11-08 Genovoxx Gmbh Nukleosid-triphosphat-konjugate und methoden zu deren anwendung
US9062091B2 (en) * 2012-02-15 2015-06-23 Pacific Biosciences Of California, Inc. Polymerase enzyme substrates with protein shield
EP3589752B1 (en) 2017-01-26 2021-05-19 QIAGEN GmbH Method for generating a sequencing library
WO2018144854A1 (en) 2017-02-02 2018-08-09 Caris Science, Inc. Targeted oligonucleotides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090186343A1 (en) * 2003-01-28 2009-07-23 Visigen Biotechnologies, Inc. Methods for preparing modified biomolecules, modified biomolecules and methods for using same
WO2005111240A2 (en) * 2004-04-30 2005-11-24 Li-Cor, Inc. Field-switch sequencing
WO2009073201A2 (en) * 2007-12-04 2009-06-11 Pacific Biosciences Of California, Inc. Alternate labeling strategies for single molecule sequencing

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2020243017A1 *

Also Published As

Publication number Publication date
GB2597398B (en) 2024-03-06
KR20250096873A (ko) 2025-06-27
JP7542000B2 (ja) 2024-08-29
AU2022291540B2 (en) 2024-11-14
IL301380B1 (en) 2025-01-01
IL287528B1 (en) 2023-04-01
IL316901A (en) 2025-01-01
SG11202112049VA (en) 2021-12-30
IL301380A (en) 2023-05-01
DE112020002516T5 (de) 2022-03-24
CA3137120A1 (en) 2020-12-03
CN113939601A (zh) 2022-01-14
JP2022535187A (ja) 2022-08-05
IL301380B2 (en) 2025-05-01
IL287528B2 (en) 2023-08-01
IL287528A (en) 2021-12-01
DE112020002516B4 (de) 2025-03-13
EP3947731A1 (en) 2022-02-09
KR20230165871A (ko) 2023-12-05
AU2025200380A1 (en) 2025-02-06
GB202115667D0 (en) 2021-12-15
KR102823471B1 (ko) 2025-06-19
KR20210144929A (ko) 2021-11-30
AU2020285657A1 (en) 2021-11-18
WO2020243017A1 (en) 2020-12-03
KR102607124B1 (ko) 2023-11-29
AU2020285657B2 (en) 2022-10-06
AU2022291540A1 (en) 2023-02-02
JP2024174880A (ja) 2024-12-17
GB2597398A (en) 2022-01-26

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