GB2597398A - Multivalent binding composition for nucleic acid analysis - Google Patents
Multivalent binding composition for nucleic acid analysis Download PDFInfo
- Publication number
- GB2597398A GB2597398A GB2115667.4A GB202115667A GB2597398A GB 2597398 A GB2597398 A GB 2597398A GB 202115667 A GB202115667 A GB 202115667A GB 2597398 A GB2597398 A GB 2597398A
- Authority
- GB
- United Kingdom
- Prior art keywords
- nucleotide
- polymer
- nucleic acid
- acid sequence
- target nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract 33
- 239000000203 mixture Substances 0.000 title claims abstract 23
- 108020004707 nucleic acids Proteins 0.000 title claims 5
- 102000039446 nucleic acids Human genes 0.000 title claims 5
- 239000002773 nucleotide Substances 0.000 claims abstract 79
- 125000003729 nucleotide group Chemical group 0.000 claims abstract 50
- 238000000034 method Methods 0.000 claims 52
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 26
- 229920000642 polymer Polymers 0.000 claims 16
- 239000002777 nucleoside Chemical class 0.000 claims 12
- 150000003833 nucleoside derivatives Chemical class 0.000 claims 12
- 230000000295 complement effect Effects 0.000 claims 7
- 239000000758 substrate Substances 0.000 claims 7
- 238000010494 dissociation reaction Methods 0.000 claims 6
- 230000005593 dissociations Effects 0.000 claims 6
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 4
- 238000002073 fluorescence micrograph Methods 0.000 claims 4
- 239000003153 chemical reaction reagent Substances 0.000 claims 3
- 150000002500 ions Chemical class 0.000 claims 3
- 108090001008 Avidin Proteins 0.000 claims 2
- 241001264766 Callistemon Species 0.000 claims 2
- OHOQEZWSNFNUSY-UHFFFAOYSA-N Cy3-bifunctional dye zwitterion Chemical group O=C1CCC(=O)N1OC(=O)CCCCCN1C2=CC=C(S(O)(=O)=O)C=C2C(C)(C)C1=CC=CC(C(C1=CC(=CC=C11)S([O-])(=O)=O)(C)C)=[N+]1CCCCCC(=O)ON1C(=O)CCC1=O OHOQEZWSNFNUSY-UHFFFAOYSA-N 0.000 claims 2
- 108020004414 DNA Proteins 0.000 claims 2
- 239000002202 Polyethylene glycol Substances 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 2
- 229960002685 biotin Drugs 0.000 claims 2
- 235000020958 biotin Nutrition 0.000 claims 2
- 239000011616 biotin Substances 0.000 claims 2
- 239000000872 buffer Substances 0.000 claims 2
- 238000007385 chemical modification Methods 0.000 claims 2
- 239000000412 dendrimer Substances 0.000 claims 2
- 229920000736 dendritic polymer Polymers 0.000 claims 2
- 230000001419 dependent effect Effects 0.000 claims 2
- 238000001514 detection method Methods 0.000 claims 2
- 238000005259 measurement Methods 0.000 claims 2
- 230000035772 mutation Effects 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 229920001223 polyethylene glycol Polymers 0.000 claims 2
- 230000010076 replication Effects 0.000 claims 2
- 239000007991 ACES buffer Substances 0.000 claims 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims 1
- 229910001424 calcium ion Inorganic materials 0.000 claims 1
- 238000003384 imaging method Methods 0.000 claims 1
- 229910001425 magnesium ion Inorganic materials 0.000 claims 1
- 230000002688 persistence Effects 0.000 claims 1
- 239000003880 polar aprotic solvent Substances 0.000 claims 1
- 238000009738 saturating Methods 0.000 claims 1
- 229910001427 strontium ion Inorganic materials 0.000 claims 1
- 230000003993 interaction Effects 0.000 abstract 2
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000006911 enzymatic reaction Methods 0.000 abstract 1
- 238000010348 incorporation Methods 0.000 abstract 1
- 238000007899 nucleic acid hybridization Methods 0.000 abstract 1
- 239000002245 particle Substances 0.000 abstract 1
- 230000004850 protein–protein interaction Effects 0.000 abstract 1
- 238000012163 sequencing technique Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/157—A reaction step characterised by the number of molecules incorporated or released
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
Abstract
Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.
Claims (73)
1. A method of determining an identity of a nucleotide in a target nucleic acid sequence comprising: a. providing a composition comprising: i. two or more copies of said target nucleic acid sequence; ii. two or more primer nucleic acid molecules that are complementary to one or more regions of said target nucleic acid sequence; and iii. two or more polymerase molecules; b. contacting said composition with a polymer nucleotide conjugate under conditions sufficient to allow a multivalent binding complex to be formed between said polymer- nucleotide conjugate and said two or more copies of said target nucleic acid sequence in said composition of (a), wherein the polymer-nucleotide conjugate comprises two or more copies of a nucleotide moiety and optionally one or more detectable labels; and c. detecting said multivalent binding complex, thereby determining the identity of said nucleotide in the target nucleic acid sequence.
2. The method of claim 1, wherein the target nucleic acid sequence is DNA.
3. The method of claim 1 or claim 2, wherein the detection of the multivalent binding complex is performed in the absence of unbound or solution-borne polymer nucleotide conjugates.
4. The method of any one of claims 1 to 3, wherein the target nucleic acid sequence has been replicated or amplified or has been produced by replication or amplification.
5. The method of any one of claims 1 to 4, wherein the one or more detectable labels are fluorescent labels.
6. The method of any one of claims 1 to 5, wherein detecting the multivalent complex comprises a fluorescence measurement.
7. The method of any one of claims 1 to 6, wherein the contacting comprises use of one type of polymer-nucleotide conjugate.
8. The method of any one of claims 1 to 7, wherein the contacting comprises use of two or more types of polymer-nucleotide conjugates.
9. The method of claim 8, wherein each type of the two or more types of polymer-nucleotide conjugate comprises a different type of nucleotide moiety.
10. The method of claim 9, wherein the contacting comprises use of three types of polymer- nucleotide conjugate and wherein each type of the three types of polymer-nucleotide conjugate comprises a different type of nucleotide moiety.
11. The method of any one of claims 1 to 10, wherein the polymer-nucleotide conjugate comprises a blocked nucleotide moiety.
12. The method of claim 11, wherein the blocked nucleotide is a 3 '-0-azidomethyl nucleotide, a 3 '- 0-methyl nucleotide, or a 3 '-0-alkyl hydroxylamine nucleotide.
13. The method of any one of claims 1 to 12, wherein said contacting occurs in the presence of an ion that stabilizes said multivalent binding complex.
14. The method of any one of claims 1 to 13, wherein the contacting is done in the presence of strontium ions, magnesium ions, calcium ions, or any combination thereof.
15. The method of any one of claims 1 to 14, wherein the polymerase molecules are catalytically inactive.
16. The method of any one of claims 1 to 15, wherein the polymerase molecules have been rendered catalytically inactive by mutation or chemical modification.
17. The method of any one of claims 1 to 16, wherein the polymerase molecules have been rendered catalytically inactive by the absence of a necessary ion or cofactor.
18. The method of any one of claims 1 to 17, wherein the polymerase molecules are catalytically active.
19. The method of any one of claims 1 to 18, wherein the polymer-nucleotide conjugate does not comprise a blocked nucleotide moiety.
20. The method of any one of claims 1 to 19, wherein the multivalent binding complex has a persistence time of greater than 2 seconds.
21. The method of any one of claims 1 to 20, wherein the method can be carried out at a temperature within a range of 25°C to 62°C.
22. The method of any one of claims 1 to 21, wherein the polymer-nucleotide conjugate further comprises one or more fluorescent labels and the two or more copies of the target nucleic acid sequence are deposited on, attached to, or hybridized to a surface, wherein a fluorescence image of the multivalent binding complex on the surface has a contrast to noise ratio in the detecting step of greater than 20.
23. The method of any one of claims 1 to 22, wherein the composition of (a) is deposited on a surface using a buffer that incorporates a polar aprotic solvent.
24. The method of any one of claims 1 to 23, wherein the contacting is performed under a condition that stabilizes said multivalent binding complex when said nucleotide moiety is complementary to a next base of said target nucleic acid sequence and destabilizes said multivalent binding complex when said nucleotide moiety is not complementary to said next base of said target nucleic acid sequence.
25. The method of any one of claims 1 to 24, wherein said polymer-nucleotide conjugate comprises a polymer having a plurality of branches and said two or more nucleotide moieties are attached to said branches.
26. The method of claim 25, wherein said polymer has a star, comb, cross-linked, bottle brush, or dendrimer configuration.
27. The method of any one of claims 1 to 26, wherein said polymer-nucleotide conjugate comprises one or more binding groups selected from the group consisting of an avidin, a biotin, an affinity tag, and combinations thereof.
28. The method of any one of claims 1 to 27, further comprising a dissociation step that destabilizes said multivalent binding complex formed between the composition of (a) and the polymer- nucleotide conjugate, said dissociation step enabling removal of said polymer-nucleotide conjugate.
29. The method of claim 28, further comprising an extension step to incorporate a nucleotide that is complementary to a next base of the target nucleic acid sequence into said two or more primer nucleic acid molecules.
30. The method of claim 29, wherein the extension step occurs concurrently with or after said dissociation step.
31. A method of determining an identity of a nucleotide in a target nucleic acid sequence comprising: a. providing a composition comprising: i. two or more copies of said target nucleic acid sequence; ii. two or more primer nucleic acid molecules that are complementary to one or more regions of said target nucleic acid sequence; and iii. two or more polymerase molecules; b. contacting said composition with a polymer nucleotide conjugate under conditions sufficient to allow a multivalent complex to be formed between said polymer-nucleotide conjugate and said two or more copies of said target nucleic acid sequence in said composition of (a), wherein the polymer-nucleotide conjugate comprises two or more copies of a reversibly terminated nucleotide moiety and optionally one or more cleavable detectable labels; and c. detecting said multivalent complex, thereby determining the identity of said nucleotide in the target nucleic acid sequence.
32. The method of claim 31, wherein the target nucleic acid sequence is DNA.
33. The method of claim 31 or claim 32, further comprising contacting the composition of (a) with reversibly terminated nucleotides or polymer-nucleotide conjugates comprising two or more copies of a reversibly terminated nucleotide following the detection of said multivalent binding complex.
34. The method of any one of claims 31 to 33, wherein the target nucleic acid sequence has been replicated or amplified or has been produced by replication or amplification.
35. The method of any one of claims 31 to 34, wherein the one or more detectable labels are fluorescent labels.
36. The method of any one of claims 31 to 35, wherein detecting the multivalent complex comprises a fluorescence measurement.
37. The method of any one of claims 31 to 36, wherein the contacting comprises use of one type of polymer-nucleotide conjugate.
38. The method of any one of claims 31 to 37, wherein the contacting comprises use of two or more types of polymer-nucleotide conjugates.
39. The method of claim 38, wherein each type of the two or more types of polymer-nucleotide conjugate comprises a different type of nucleotide moiety.
40. The method of claim 39, wherein the contacting comprises use of three types of polymer- nucleotide conjugate and wherein each type of the three types of polymer-nucleotide conjugate comprises a different type of nucleotide moiety.
41. The method of any one of claims 31 to 40, wherein the polymer-nucleotide conjugate comprises a blocked nucleotide moiety.
42. The method of claim 41, wherein the blocked nucleotide is a 3 '-0-azidomethyl, 3 '-0-methyl, or 3 '-0-alkyl hydroxylamine.
43. The method of any one of claims 31 to 42, wherein said contacting occurs in the presence of an ion that stabilizes said multivalent binding complex.
44. The method of any one of claims 31 to 43, wherein the polymerase molecules are catalytically inactive.
45. The method of any one of claims 31 to 44, wherein the polymerase molecules have been rendered catalytically inactive by mutation or chemical modification.
46. The method of any one of claims 31 to 45, wherein the polymerase molecules are catalytically active.
47. The method of any one of claims 31 to 46, wherein the polymer-nucleotide conjugate does not comprise a blocked nucleotide moiety.
48. The method of any one of claims 31 to 47, wherein the method can be carried out at a temperature within a range of 25°C to 80°C.
49. The method of any one of claims 31 to 48, wherein the polymer-nucleotide conjugate further comprises one or more fluorescent labels and the two or more copies of the target nucleic acid sequence are deposited on, attached to, or hybridized to a surface, wherein a fluorescence image of the multivalent binding complex on the surface has a contrast to noise ratio in the detecting step of greater than 20.
50. A system comprising: a) one or more computer processors individually or collectively programmed to implement a method comprising: i) contacting a substrate comprising multiple copies of a target nucleic acid sequence tethered to a surface of the substrate with a reagent comprising a polymerase and one or more primer nucleic acid sequences that are complementary to one or more regions of said target nucleic acid sequence to form a primed target nucleic acid sequence; ii) contacting the substrate surface with a reagent comprising a polymer nucleotide conjugate under conditions sufficient to allow a multivalent binding complex to be formed between said polymer-nucleotide conjugate and two or more copies of said primed target nucleic acid sequence, wherein the polymer-nucleotide conjugate comprises two or more copies of a known nucleotide moiety and a detectable label; iii) acquiring and processing an image of the substrate surface to detect said multivalent binding complex, thereby determining the identity of a nucleotide in the target nucleic acid sequence.
51. The system of claim 50, further comprising a fluidics module configured to deliver a series of reagents to the substrate surface in a specified sequence and for specified time intervals.
52. The system of claim 50 or claim 51, further comprising an imaging module configured to acquire images of the substrate surface.
53. The system of any one of claims 50 to 52, wherein (ii) and (iii) are repeated two or more times thereby determining the identity of a series of two or more nucleotides in the target nucleic acid sequence.
54. The system of any one of claims 50 to 53, wherein the series of steps further comprises a dissociation step that destabilizes said multivalent binding complex, said dissociation step enabling removal of said polymer-nucleotide conjugate.
55. The system of claim 54, wherein the series of steps further comprises an extension step to incorporate a nucleotide that is complementary to a next base of the target nucleic acid sequence into said two or more primer nucleic acid molecules.
56. The system of claim 55, wherein the extension step occurs concurrently with or after said dissociation step.
57. The system of any one of claims 50 to 56, wherein the detectable label comprises a fluorophore and the images comprise fluorescence images.
58. The system of claim 57, wherein the fluorescence images of the multivalent binding complex on the substrate surface has a contrast-to-noise ratio of greater than 20 when the fluorophore is cyanine dye 3 (Cy3) and the image is acquired using an inverted fluorescence microscope equipped with a 20X objective, NA = 0.75, dichroic mirror optimized for 532 nm light, a bandpass filter optimized for Cyanine dye-3 emission, and a camera, under non-signal saturating conditions while the surface is immersed in 25 mM ACES, pH 7.4 buffer.
59. The system of any one of claims 50 to 58, wherein the series of steps is completed in less than 60 minutes.
60. The system of any one of claims 50 to 59, wherein the series of steps is completed in less than 30 minutes.
61. The system of any one of claims 50 to 60, wherein the series of steps is completed in less than 10 minutes.
62. The system of any one of claims 50 to 61, wherein an accuracy of base-calling is characterized by a Q-score of greater than 25 for at least 80% of the nucleotide identities determined.
63. The system of any one of claims 50 to 62, wherein an accuracy of base-calling is characterized by a Q-score of greater than 30 for at least 80% of the nucleotide identities determined.
64. The system of any one of claims 50 to 63, wherein an accuracy of base-calling is characterized by a Q-score of greater than 40 for at least 80% of the nucleotide identities determined.
65. A composition comprising: a) a polymer core; and b) two or more nucleotide, nucleotide analog, nucleoside, or nucleoside analog moieties attached to the polymer core; wherein the length of the linker is dependent on the nucleotide, nucleotide analog, nucleoside, or nucleoside analog moiety that is attached to the polymer core.
66. A composition comprising: a) a mixture of polymer-nucleotide conjugates, wherein each polymer-nucleotide conjugate comprises: i) a polymer core; and ii) two or more nucleotide, nucleotide analog, nucleoside, or nucleoside analog moieties attached to the polymer core, wherein the length of the linker is dependent on the nucleotide, nucleotide analog, nucleoside, or nucleoside analog moiety that is attached to the polymer core; and wherein the mixture comprises polymer-nucleotide conjugates having at least two different types of attached nucleotide, nucleotide analog, nucleoside, or nucleoside analog moiety.
67. The composition of claim 65 or claim 66, wherein the polymer core comprises a polymer having a plurality of branches and the two or more nucleotide, nucleotide analog, nucleoside, or nucleoside analog moieties are attached to said branches.
68. The composition of claim 67, wherein the polymer has a star, comb, cross-linked, bottle brush, or dendrimer configuration.
69. The composition of any one of claims 65 to 68, wherein the polymer-nucleotide conjugate comprises one or more binding groups selected from the group consisting of an avidin, a biotin, an affinity tag, and combinations thereof.
70. The composition of any one of claims 65 to 69, wherein the polymer core comprises a branched polyethylene glycol (PEG) molecule.
71. The composition of any one of claims 65 to 70, wherein the polymer-nucleotide conjugate comprises a blocked nucleotide moiety.
72. The composition of claim 71, wherein the blocked nucleotide is a 3 '-0-azidomethyl nucleotide, a 3 '-0-methyl nucleotide, or a 3 '-0-alkyl hydroxylamine nucleotide.
73. The composition of any one of claims 65 to 72, wherein the polymer-nucleotide conjugate further comprises one or more fluorescent labels.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962852876P | 2019-05-24 | 2019-05-24 | |
| US201962897172P | 2019-09-06 | 2019-09-06 | |
| US16/579,794 US10768173B1 (en) | 2019-09-06 | 2019-09-23 | Multivalent binding composition for nucleic acid analysis |
| PCT/US2020/034409 WO2020243017A1 (en) | 2019-05-24 | 2020-05-22 | Multivalent binding composition for nucleic acid analysis |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| GB202115667D0 GB202115667D0 (en) | 2021-12-15 |
| GB2597398A true GB2597398A (en) | 2022-01-26 |
| GB2597398B GB2597398B (en) | 2024-03-06 |
Family
ID=73554179
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB2115667.4A Active GB2597398B (en) | 2019-05-24 | 2020-05-22 | Multivalent binding composition for nucleic acid analysis |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP3947731A4 (en) |
| JP (1) | JP2022535187A (en) |
| KR (2) | KR102607124B1 (en) |
| CN (1) | CN113939601A (en) |
| AU (2) | AU2020285657B2 (en) |
| CA (1) | CA3137120A1 (en) |
| DE (1) | DE112020002516T5 (en) |
| GB (1) | GB2597398B (en) |
| IL (2) | IL301380A (en) |
| SG (1) | SG11202112049VA (en) |
| WO (1) | WO2020243017A1 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2022512328A (en) | 2018-12-07 | 2022-02-03 | エレメント バイオサイエンシーズ，インク． | Flow cell device and its use |
| US11287422B2 (en) | 2019-09-23 | 2022-03-29 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
| US11053540B1 (en) | 2020-01-17 | 2021-07-06 | Element Biosciences, Inc. | High performance fluorescence imaging module for genomic testing assay |
| US11198121B1 (en) | 2020-06-10 | 2021-12-14 | Element Biosciences, Inc. | Flow cell systems and devices |
| GB2617481A (en) | 2020-10-30 | 2023-10-11 | Element Biosciences Inc | Reagents for massively parallel nucleic acid sequencing |
| KR20230153706A (en) | 2022-04-29 | 2023-11-07 | 연세대학교 산학협력단 | A composition for detecting or isolating nucleic acids and a method for detecting or isolating nucleic acids using the same |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005111240A2 (en) * | 2004-04-30 | 2005-11-24 | Li-Cor, Inc. | Field-switch sequencing |
| WO2009073201A2 (en) * | 2007-12-04 | 2009-06-11 | Pacific Biosciences Of California, Inc. | Alternate labeling strategies for single molecule sequencing |
| US20090186343A1 (en) * | 2003-01-28 | 2009-07-23 | Visigen Biotechnologies, Inc. | Methods for preparing modified biomolecules, modified biomolecules and methods for using same |
| US20100093992A1 (en) * | 2004-11-05 | 2010-04-15 | Dmitry Cherkasov | Macromolecular Nucleotide Compounds and Methods for Using the Same |
| WO2012027625A2 (en) * | 2010-08-25 | 2012-03-01 | Pacific Biosciences Of California, Inc. | Scaffold-based polymerase enzyme substrates |
| WO2013123258A1 (en) * | 2012-02-15 | 2013-08-22 | Pacific Biosciences Of California, Inc. | Polymerase enzyme substrates with protein shield |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2705161A1 (en) * | 2011-05-04 | 2014-03-12 | Genovoxx GmbH | Nucleoside-triphosphate conjugate and methods for the use thereof |
-
2020
- 2020-05-22 KR KR1020217037728A patent/KR102607124B1/en active IP Right Grant
- 2020-05-22 DE DE112020002516.0T patent/DE112020002516T5/en active Pending
- 2020-05-22 CA CA3137120A patent/CA3137120A1/en active Pending
- 2020-05-22 EP EP20815612.5A patent/EP3947731A4/en active Pending
- 2020-05-22 IL IL301380A patent/IL301380A/en unknown
- 2020-05-22 JP JP2021561845A patent/JP2022535187A/en active Pending
- 2020-05-22 GB GB2115667.4A patent/GB2597398B/en active Active
- 2020-05-22 KR KR1020237040302A patent/KR20230165871A/en active Application Filing
- 2020-05-22 WO PCT/US2020/034409 patent/WO2020243017A1/en unknown
- 2020-05-22 CN CN202080042516.6A patent/CN113939601A/en active Pending
- 2020-05-22 SG SG11202112049VA patent/SG11202112049VA/en unknown
- 2020-05-22 AU AU2020285657A patent/AU2020285657B2/en active Active
-
2021
- 2021-10-24 IL IL287528A patent/IL287528B2/en unknown
-
2022
- 2022-12-22 AU AU2022291540A patent/AU2022291540A1/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090186343A1 (en) * | 2003-01-28 | 2009-07-23 | Visigen Biotechnologies, Inc. | Methods for preparing modified biomolecules, modified biomolecules and methods for using same |
| WO2005111240A2 (en) * | 2004-04-30 | 2005-11-24 | Li-Cor, Inc. | Field-switch sequencing |
| US20100093992A1 (en) * | 2004-11-05 | 2010-04-15 | Dmitry Cherkasov | Macromolecular Nucleotide Compounds and Methods for Using the Same |
| WO2009073201A2 (en) * | 2007-12-04 | 2009-06-11 | Pacific Biosciences Of California, Inc. | Alternate labeling strategies for single molecule sequencing |
| WO2012027625A2 (en) * | 2010-08-25 | 2012-03-01 | Pacific Biosciences Of California, Inc. | Scaffold-based polymerase enzyme substrates |
| WO2013123258A1 (en) * | 2012-02-15 | 2013-08-22 | Pacific Biosciences Of California, Inc. | Polymerase enzyme substrates with protein shield |
Non-Patent Citations (2)
| Title |
|---|
| Anderson, J P. Et al "Flurescent Structural DNA Nanoballs Functionalized with Phospate - Lineked Nucleotide Triphosphates" Nano Letters 2010 10(3): 788-792. doi: 10.1021/nl9039718. Abstract, second last paragraph, Fig 3A * |
| Technology Spotlight: Illumina Sequencing, 2010, downloaded form https://www.illumina.com/documents/products/techspotlights/techspotlight_sequencing. pdf on 31/07.2020 * |
Also Published As
| Publication number | Publication date |
|---|---|
| KR102607124B1 (en) | 2023-11-29 |
| KR20230165871A (en) | 2023-12-05 |
| EP3947731A4 (en) | 2022-06-29 |
| AU2020285657B2 (en) | 2022-10-06 |
| CN113939601A (en) | 2022-01-14 |
| KR20210144929A (en) | 2021-11-30 |
| IL287528B2 (en) | 2023-08-01 |
| AU2020285657A1 (en) | 2021-11-18 |
| GB202115667D0 (en) | 2021-12-15 |
| DE112020002516T5 (en) | 2022-03-24 |
| JP2022535187A (en) | 2022-08-05 |
| IL287528A (en) | 2021-12-01 |
| EP3947731A1 (en) | 2022-02-09 |
| GB2597398B (en) | 2024-03-06 |
| AU2022291540A1 (en) | 2023-02-02 |
| SG11202112049VA (en) | 2021-12-30 |
| IL287528B1 (en) | 2023-04-01 |
| WO2020243017A1 (en) | 2020-12-03 |
| IL301380A (en) | 2023-05-01 |
| CA3137120A1 (en) | 2020-12-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| GB2597398A (en) | Multivalent binding composition for nucleic acid analysis | |
| KR102187291B1 (en) | Chemical composition and how to use it | |
| AU2017268257B2 (en) | Methods for detecting target nucleic acids in a sample | |
| JP5951755B2 (en) | Improved quantitative nuclease protection assay (qNPA) and quantitative nuclease protection sequencing (qNPS) methods | |
| AU2018375160A1 (en) | Sequencing of nucleic acids by emergence | |
| US8058005B2 (en) | Method for single nucleotide polymorphism and mutation detection using real time polymerase chain reaction microarray | |
| EP2647426A1 (en) | Replication of distributed nucleic acid molecules with preservation of their relative distribution through hybridization-based binding | |
| CA2738596C (en) | Selective processing of biological material on a microarray substrate | |
| JP2018529314A5 (en) | ||
| US20090062132A1 (en) | Alternative nucleic acid sequencing methods | |
| US20090005256A1 (en) | Analysis of Encoded Chemical Libraries | |
| US20230175047A1 (en) | Array and method for detecting spatial information of nucleic acids | |
| US9145582B2 (en) | Microarray techniques for nucleic acid expression analyses | |
| CN106536735A (en) | Probe set for analyzing a dna sample and method for using the same | |
| KR20210061962A (en) | Chemical composition and method of use thereof | |
| KR20210084441A (en) | Methods and compositions for identifying ligands using indexes and barcodes on arrays | |
| US20100279885A1 (en) | Oligonucleotide microarray for identification of pathogens | |
| US10392652B2 (en) | Micro RNA detection method using two primers to produce an amplified double stranded DNA fragment having a single stranded region at one end | |
| US20100216146A1 (en) | Methods and Kits for Hybridizing Multiple PNA Probe Panels to Nucleic Acid Samples | |
| EP3887545A1 (en) | Sequencing by coalascence | |
| US20070190618A1 (en) | Methods and compositions for detecting viral nucleic acid in a cell | |
| JPWO2020243017A5 (en) | ||
| US20200308636A1 (en) | Method for detecting polynucleotide using FRET-PAINT | |
| CN116515975A (en) | Method for screening for aptamers using sequencing | |
| JPWO2006083040A1 (en) | Nucleic acid detection method using nucleic acid microarray |