DE112007002932T8 - Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren - Google Patents

Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren Download PDF

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DE112007002932T8
DE112007002932T8 DE200711002932 DE112007002932T DE112007002932T8 DE 112007002932 T8 DE112007002932 T8 DE 112007002932T8 DE 200711002932 DE200711002932 DE 200711002932 DE 112007002932 T DE112007002932 T DE 112007002932T DE 112007002932 T8 DE112007002932 T8 DE 112007002932T8
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cleavable
reversible
synthesis
dna sequencing
fluorescent nucleotide
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DE112007002932T5 (de
DE112007002932B4 (de
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Jingyue Ju
Dae Hyun Kim
Lanrong Bi
Qinglin Meng
Xiaoxu Li
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Columbia University in the City of New York
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
DE112007002932.3T 2006-12-01 2007-11-30 Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren Active DE112007002932B4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US87224006P 2006-12-01 2006-12-01
US60/872,240 2006-12-01
PCT/US2007/024646 WO2008069973A2 (en) 2006-12-01 2007-11-30 Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators

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DE112007002932T5 DE112007002932T5 (de) 2009-10-08
DE112007002932T8 true DE112007002932T8 (de) 2009-11-26
DE112007002932B4 DE112007002932B4 (de) 2015-08-06

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US (8) US7883869B2 (de)
DE (1) DE112007002932B4 (de)
GB (1) GB2457402B (de)
WO (1) WO2008069973A2 (de)

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JP6456816B2 (ja) 2012-04-09 2019-01-23 ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク ナノ細孔の調製方法およびその使用
EP3674412A1 (de) 2012-06-20 2020-07-01 The Trustees of Columbia University in the City of New York Nukleinsäuresequenzierung durch nanoporendetektion von markierungsmolekülen
AU2014215764A1 (en) 2013-02-05 2015-09-17 Victoria Link Limited Novel bio-sensor for the detection of small molecules
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
EP2971051A4 (de) 2013-03-15 2017-03-01 The Trustees of Columbia University in the City of New York Verfahren zur erkennung mehrerer vordefinierter verbindungen in einer probe
US10718011B2 (en) 2014-02-12 2020-07-21 The Trustees Of Columbia University In The City Of New York Single molecule electronic multiplex SNP assay and PCR analysis

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GB2457402B (en) 2011-10-19
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US9528151B2 (en) 2016-12-27
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US20210381043A1 (en) 2021-12-09
US20120021408A1 (en) 2012-01-26
US10000801B2 (en) 2018-06-19
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US20100092952A1 (en) 2010-04-15
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DE112007002932B4 (de) 2015-08-06
US20140315191A1 (en) 2014-10-23
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US11939631B2 (en) 2024-03-26
US20200208211A1 (en) 2020-07-02

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