DE1054405B - Verfahren zur Gewinnung von Urokinasekonzentraten - Google Patents
Verfahren zur Gewinnung von UrokinasekonzentratenInfo
- Publication number
- DE1054405B DE1054405B DEL25202A DEL0025202A DE1054405B DE 1054405 B DE1054405 B DE 1054405B DE L25202 A DEL25202 A DE L25202A DE L0025202 A DEL0025202 A DE L0025202A DE 1054405 B DE1054405 B DE 1054405B
- Authority
- DE
- Germany
- Prior art keywords
- urokinase
- value
- solution
- adsorbate
- electrolyte solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 title claims description 38
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 title claims description 38
- 229960005356 urokinase Drugs 0.000 title claims description 38
- 238000000034 method Methods 0.000 title claims description 22
- 239000012141 concentrate Substances 0.000 title claims description 7
- 238000004519 manufacturing process Methods 0.000 title claims 2
- 239000000243 solution Substances 0.000 claims description 28
- 239000008151 electrolyte solution Substances 0.000 claims description 17
- 210000002700 urine Anatomy 0.000 claims description 17
- 238000010828 elution Methods 0.000 claims description 15
- 239000002156 adsorbate Substances 0.000 claims description 12
- 239000003463 adsorbent Substances 0.000 claims description 11
- 238000001179 sorption measurement Methods 0.000 claims description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 8
- 239000011347 resin Substances 0.000 claims description 8
- 229920005989 resin Polymers 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000000337 buffer salt Substances 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 6
- 230000015572 biosynthetic process Effects 0.000 claims description 5
- 150000001768 cations Chemical class 0.000 claims description 5
- 239000000741 silica gel Substances 0.000 claims description 5
- 229910002027 silica gel Inorganic materials 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 4
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 3
- 239000003729 cation exchange resin Substances 0.000 claims description 3
- 238000005194 fractionation Methods 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 claims description 2
- 229920002472 Starch Polymers 0.000 claims description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 2
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 102000016938 Catalase Human genes 0.000 claims 1
- 108010053835 Catalase Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004142 Trypsin Human genes 0.000 claims 1
- 108090000631 Trypsin Proteins 0.000 claims 1
- 239000006103 coloring component Substances 0.000 claims 1
- 239000013078 crystal Substances 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 claims 1
- 239000012588 trypsin Substances 0.000 claims 1
- 229940021013 electrolyte solution Drugs 0.000 description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonium chloride Substances [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 2
- 102000008946 Fibrinogen Human genes 0.000 description 2
- 108010049003 Fibrinogen Proteins 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 description 2
- TZCXTZWJZNENPQ-UHFFFAOYSA-L barium sulfate Chemical compound [Ba+2].[O-]S([O-])(=O)=O TZCXTZWJZNENPQ-UHFFFAOYSA-L 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- 229940012952 fibrinogen Drugs 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- DPEYHNFHDIXMNV-UHFFFAOYSA-N (9-amino-3-bicyclo[3.3.1]nonanyl)-(4-benzyl-5-methyl-1,4-diazepan-1-yl)methanone dihydrochloride Chemical compound Cl.Cl.CC1CCN(CCN1Cc1ccccc1)C(=O)C1CC2CCCC(C1)C2N DPEYHNFHDIXMNV-UHFFFAOYSA-N 0.000 description 1
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 1
- -1 For example Chemical compound 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 229910052910 alkali metal silicate Inorganic materials 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 239000008119 colloidal silica Substances 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical class O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000003527 fibrinolytic agent Substances 0.000 description 1
- 230000003480 fibrinolytic effect Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 201000005060 thrombophlebitis Diseases 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB353848X | 1955-07-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE1054405B true DE1054405B (de) | 1959-04-09 |
Family
ID=10377142
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DEL25202A Pending DE1054405B (de) | 1955-07-01 | 1956-06-29 | Verfahren zur Gewinnung von Urokinasekonzentraten |
Country Status (7)
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1263659B (de) * | 1964-07-01 | 1968-03-21 | Pharmexbio S A | Verfahren zur Gewinnung von Urokinase |
| DE1289809B (de) * | 1965-10-01 | 1969-02-27 | Green Cross Corp | Verfahren zur Gewinnung von Urokinase fuer Injektionszwecke |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3256158A (en) * | 1963-03-22 | 1966-06-14 | Abbott Lab | Purification of urokinase |
| US3542646A (en) * | 1966-11-22 | 1970-11-24 | Green Cross Corp | Process for fractionally obtaining urokinase and blood coagulation accelerator in human urine |
| JPS53113080A (en) * | 1977-03-10 | 1978-10-03 | Sumitomo Chem Co Ltd | Purification of urokinase |
| WO1981001417A1 (en) * | 1979-11-13 | 1981-05-28 | S Husain | Isolation of plasminogen activators useful as therapeutic and diagnostic agents |
| USRE32271E (en) * | 1979-11-13 | 1986-10-28 | Isolation of plasminogen activators useful as therapeutic and diagnostic agents | |
| DE3439980A1 (de) * | 1984-11-02 | 1986-05-07 | Behringwerke Ag, 3550 Marburg | Verfahren zur reinigung sowie pasteurisierung von urokinase |
| US4920051A (en) * | 1988-02-03 | 1990-04-24 | Damon Biotech, Inc. | Recovery of urokinase compounds |
| CN111647585A (zh) * | 2020-07-08 | 2020-09-11 | 江苏尤里卡生物科技有限公司 | 一种尿激酶的精制方法 |
| CN111647587A (zh) * | 2020-07-08 | 2020-09-11 | 江苏尤里卡生物科技有限公司 | 一种尿激酶中间体的纯化方法 |
| CN111748545B (zh) * | 2020-07-09 | 2022-09-30 | 江苏尤里卡生物科技有限公司 | 一种用琼脂糖凝胶4b吸附尿液中尿激酶的方法 |
| CN111662896A (zh) * | 2020-07-10 | 2020-09-15 | 江苏尤里卡生物科技有限公司 | 一种粗品尿激酶的纯化方法 |
| CN115197926A (zh) * | 2022-05-25 | 2022-10-18 | 山东邦凯新材料有限公司 | 一种利用改性树脂提取尿激酶的制备方法 |
| CN115386563A (zh) * | 2022-09-20 | 2022-11-25 | 河南省尤里卡生物科技有限公司 | 一种快速制备尿激酶原料的方法 |
| CN117305052B (zh) * | 2023-09-20 | 2024-08-20 | 河北利江生物科技有限公司 | 一种人源蛋白制备装置及方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2442452A (en) * | 1945-12-18 | 1948-06-01 | Us Agriculture | Method of isolating lysozyme from its naturally occurring mixtures with other biologic components |
-
0
- BE BE549167D patent/BE549167A/xx unknown
- NL NL96631D patent/NL96631C/xx active
-
1955
- 1955-07-01 GB GB19168/55A patent/GB802326A/en not_active Expired
-
1956
- 1956-06-25 US US593363A patent/US2983647A/en not_active Expired - Lifetime
- 1956-06-29 CH CH353848D patent/CH353848A/de unknown
- 1956-06-29 FR FR1209859D patent/FR1209859A/fr not_active Expired
- 1956-06-29 DE DEL25202A patent/DE1054405B/de active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2442452A (en) * | 1945-12-18 | 1948-06-01 | Us Agriculture | Method of isolating lysozyme from its naturally occurring mixtures with other biologic components |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1263659B (de) * | 1964-07-01 | 1968-03-21 | Pharmexbio S A | Verfahren zur Gewinnung von Urokinase |
| DE1289809B (de) * | 1965-10-01 | 1969-02-27 | Green Cross Corp | Verfahren zur Gewinnung von Urokinase fuer Injektionszwecke |
Also Published As
| Publication number | Publication date |
|---|---|
| GB802326A (en) | 1958-10-01 |
| US2983647A (en) | 1961-05-09 |
| BE549167A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | |
| NL96631C (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | |
| CH353848A (de) | 1961-04-30 |
| FR1209859A (fr) | 1960-03-04 |
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