DE10318644A1 - Transgene Pflanzen mit kontrollierter Weitergabe eines Merkmals an die Nachkommen - Google Patents
Transgene Pflanzen mit kontrollierter Weitergabe eines Merkmals an die NachkommenInfo
- Publication number
- DE10318644A1 DE10318644A1 DE2003118644 DE10318644A DE10318644A1 DE 10318644 A1 DE10318644 A1 DE 10318644A1 DE 2003118644 DE2003118644 DE 2003118644 DE 10318644 A DE10318644 A DE 10318644A DE 10318644 A1 DE10318644 A1 DE 10318644A1
- Authority
- DE
- Germany
- Prior art keywords
- nucleotide sequence
- plant
- heterologous nucleotide
- heterologous
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 230000009261 transgenic effect Effects 0.000 title claims abstract description 42
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 194
- 239000012634 fragment Substances 0.000 claims abstract description 156
- 239000002773 nucleotide Substances 0.000 claims abstract description 122
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 122
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 99
- 238000000034 method Methods 0.000 claims abstract description 70
- 210000000349 chromosome Anatomy 0.000 claims abstract description 67
- 210000004027 cell Anatomy 0.000 claims abstract description 58
- 229920001184 polypeptide Polymers 0.000 claims abstract description 52
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 52
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 51
- 230000017730 intein-mediated protein splicing Effects 0.000 claims description 42
- 230000027455 binding Effects 0.000 claims description 39
- 230000001404 mediated effect Effects 0.000 claims description 38
- 108020004414 DNA Proteins 0.000 claims description 27
- 108010020764 Transposases Proteins 0.000 claims description 26
- 102000008579 Transposases Human genes 0.000 claims description 26
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 22
- 239000013598 vector Substances 0.000 claims description 18
- 238000005520 cutting process Methods 0.000 claims description 17
- 239000002243 precursor Substances 0.000 claims description 17
- 239000003550 marker Substances 0.000 claims description 16
- 230000006798 recombination Effects 0.000 claims description 14
- 238000005215 recombination Methods 0.000 claims description 14
- 238000013518 transcription Methods 0.000 claims description 14
- 102000004190 Enzymes Human genes 0.000 claims description 13
- 108090000790 Enzymes Proteins 0.000 claims description 13
- 238000004519 manufacturing process Methods 0.000 claims description 13
- 230000035897 transcription Effects 0.000 claims description 13
- 230000009466 transformation Effects 0.000 claims description 13
- 241000589158 Agrobacterium Species 0.000 claims description 11
- 238000012546 transfer Methods 0.000 claims description 10
- 108010052160 Site-specific recombinase Proteins 0.000 claims description 9
- 230000006870 function Effects 0.000 claims description 9
- 102000018120 Recombinases Human genes 0.000 claims description 8
- 108010091086 Recombinases Proteins 0.000 claims description 8
- 230000015572 biosynthetic process Effects 0.000 claims description 8
- 230000002363 herbicidal effect Effects 0.000 claims description 7
- 239000004009 herbicide Substances 0.000 claims description 7
- 238000013519 translation Methods 0.000 claims description 6
- 238000006664 bond formation reaction Methods 0.000 claims description 4
- 238000004520 electroporation Methods 0.000 claims description 4
- 230000006833 reintegration Effects 0.000 claims description 4
- 206010021929 Infertility male Diseases 0.000 claims description 3
- 208000007466 Male Infertility Diseases 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 102100029880 Glycodelin Human genes 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims description 2
- 230000012865 response to insecticide Effects 0.000 claims description 2
- 230000003612 virological effect Effects 0.000 claims description 2
- 206010021928 Infertility female Diseases 0.000 claims 1
- 108091023040 Transcription factor Proteins 0.000 claims 1
- 102000040945 Transcription factor Human genes 0.000 claims 1
- 230000002759 chromosomal effect Effects 0.000 claims 1
- 238000004140 cleaning Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 230000007026 protein scission Effects 0.000 claims 1
- 238000010361 transduction Methods 0.000 claims 1
- 230000026683 transduction Effects 0.000 claims 1
- 238000001890 transfection Methods 0.000 claims 1
- 238000012800 visualization Methods 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 description 148
- 235000018102 proteins Nutrition 0.000 description 92
- 108700019146 Transgenes Proteins 0.000 description 23
- 230000014509 gene expression Effects 0.000 description 22
- 150000001413 amino acids Chemical class 0.000 description 15
- 235000001014 amino acid Nutrition 0.000 description 14
- 229940088598 enzyme Drugs 0.000 description 12
- 230000004927 fusion Effects 0.000 description 11
- 238000009396 hybridization Methods 0.000 description 10
- 239000013612 plasmid Substances 0.000 description 10
- 230000017105 transposition Effects 0.000 description 10
- 108091026890 Coding region Proteins 0.000 description 9
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 9
- 230000010354 integration Effects 0.000 description 9
- 229910019142 PO4 Inorganic materials 0.000 description 8
- 239000010452 phosphate Substances 0.000 description 8
- 239000005562 Glyphosate Substances 0.000 description 7
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 7
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 7
- 230000005540 biological transmission Effects 0.000 description 7
- 230000002068 genetic effect Effects 0.000 description 7
- 229940097068 glyphosate Drugs 0.000 description 7
- 239000005090 green fluorescent protein Substances 0.000 description 7
- 230000003993 interaction Effects 0.000 description 7
- 230000032361 posttranscriptional gene silencing Effects 0.000 description 7
- 108010000700 Acetolactate synthase Proteins 0.000 description 6
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 6
- 108010076504 Protein Sorting Signals Proteins 0.000 description 6
- 239000003053 toxin Substances 0.000 description 6
- 231100000765 toxin Toxicity 0.000 description 6
- 108700012359 toxins Proteins 0.000 description 6
- 230000014616 translation Effects 0.000 description 6
- 238000003776 cleavage reaction Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 108020001507 fusion proteins Proteins 0.000 description 5
- 102000037865 fusion proteins Human genes 0.000 description 5
- 210000002706 plastid Anatomy 0.000 description 5
- 230000007017 scission Effects 0.000 description 5
- 230000008685 targeting Effects 0.000 description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
- 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 4
- 102000012330 Integrases Human genes 0.000 description 4
- 108010061833 Integrases Proteins 0.000 description 4
- 241000208125 Nicotiana Species 0.000 description 4
- 241000192584 Synechocystis Species 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 210000004899 c-terminal region Anatomy 0.000 description 4
- 230000010154 cross-pollination Effects 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 210000003463 organelle Anatomy 0.000 description 4
- 210000001938 protoplast Anatomy 0.000 description 4
- 238000012163 sequencing technique Methods 0.000 description 4
- 241000219194 Arabidopsis Species 0.000 description 3
- 241000219195 Arabidopsis thaliana Species 0.000 description 3
- 101710132601 Capsid protein Proteins 0.000 description 3
- 102000053642 Catalytic RNA Human genes 0.000 description 3
- 108090000994 Catalytic RNA Proteins 0.000 description 3
- 101710094648 Coat protein Proteins 0.000 description 3
- 102100031780 Endonuclease Human genes 0.000 description 3
- 108010042407 Endonucleases Proteins 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 241000282326 Felis catus Species 0.000 description 3
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 3
- 101710125418 Major capsid protein Proteins 0.000 description 3
- 244000061176 Nicotiana tabacum Species 0.000 description 3
- 101710141454 Nucleoprotein Proteins 0.000 description 3
- 101710083689 Probable capsid protein Proteins 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 108700021044 acyl-ACP thioesterase Proteins 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 108091092562 ribozyme Proteins 0.000 description 3
- 230000001568 sexual effect Effects 0.000 description 3
- 238000011426 transformation method Methods 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- 108700028369 Alleles Proteins 0.000 description 2
- 241000203069 Archaea Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 102000015081 Blood Coagulation Factors Human genes 0.000 description 2
- 108010039209 Blood Coagulation Factors Proteins 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- 241000206602 Eukaryota Species 0.000 description 2
- 108010017464 Fructose-Bisphosphatase Proteins 0.000 description 2
- 102000027487 Fructose-Bisphosphatase Human genes 0.000 description 2
- 101150066002 GFP gene Proteins 0.000 description 2
- 101710139492 Hexitol phosphatase B Proteins 0.000 description 2
- 108010025815 Kanamycin Kinase Proteins 0.000 description 2
- 108091022912 Mannose-6-Phosphate Isomerase Proteins 0.000 description 2
- 102000048193 Mannose-6-phosphate isomerases Human genes 0.000 description 2
- 108010033272 Nitrilase Proteins 0.000 description 2
- 108010059820 Polygalacturonase Proteins 0.000 description 2
- 229920000331 Polyhydroxybutyrate Polymers 0.000 description 2
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 2
- 108010016634 Seed Storage Proteins Proteins 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 108010039811 Starch synthase Proteins 0.000 description 2
- 241000192581 Synechocystis sp. Species 0.000 description 2
- 108010022394 Threonine synthase Proteins 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 108010013829 alpha subunit DNA polymerase III Proteins 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 101150103518 bar gene Proteins 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 239000003114 blood coagulation factor Substances 0.000 description 2
- 230000007910 cell fusion Effects 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 108010031100 chloroplast transit peptides Proteins 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 210000000172 cytosol Anatomy 0.000 description 2
- 102000004419 dihydrofolate reductase Human genes 0.000 description 2
- 108091054761 ethylene receptor family Proteins 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 244000037671 genetically modified crops Species 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 238000001764 infiltration Methods 0.000 description 2
- 230000000749 insecticidal effect Effects 0.000 description 2
- 102000006495 integrins Human genes 0.000 description 2
- 108010044426 integrins Proteins 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- -1 ketohydrolase Proteins 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000021121 meiosis Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 230000000269 nucleophilic effect Effects 0.000 description 2
- 108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 2
- 230000010152 pollination Effects 0.000 description 2
- 108020001580 protein domains Proteins 0.000 description 2
- 230000016434 protein splicing Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000008707 rearrangement Effects 0.000 description 2
- 210000001082 somatic cell Anatomy 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000005026 transcription initiation Effects 0.000 description 2
- 108090000344 1,4-alpha-Glucan Branching Enzyme Proteins 0.000 description 1
- 102000003925 1,4-alpha-Glucan Branching Enzyme Human genes 0.000 description 1
- 108091000130 1-aminocyclopropane-1-carboxylate deaminase Proteins 0.000 description 1
- 108010010888 1-aminocyclopropane-1-carboxylic acid oxidase Proteins 0.000 description 1
- 108010041188 2,4-dichlorophenoxyacetic acid monooxygenase Proteins 0.000 description 1
- MWMOPIVLTLEUJO-UHFFFAOYSA-N 2-oxopropanoic acid;phosphoric acid Chemical compound OP(O)(O)=O.CC(=O)C(O)=O MWMOPIVLTLEUJO-UHFFFAOYSA-N 0.000 description 1
- 101710168820 2S seed storage albumin protein Proteins 0.000 description 1
- 102100029103 3-ketoacyl-CoA thiolase Human genes 0.000 description 1
- 108010011619 6-Phytase Proteins 0.000 description 1
- 101150012623 AGL15 gene Proteins 0.000 description 1
- 101710184601 Acetolactate synthase 2, chloroplastic Proteins 0.000 description 1
- 108010003902 Acetyl-CoA C-acyltransferase Proteins 0.000 description 1
- 241001134629 Acidothermus Species 0.000 description 1
- 101710146995 Acyl carrier protein Proteins 0.000 description 1
- 102000004539 Acyl-CoA Oxidase Human genes 0.000 description 1
- 108020001558 Acyl-CoA oxidase Proteins 0.000 description 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
- 108010074725 Alpha,alpha-trehalose phosphorylase Proteins 0.000 description 1
- 101000634158 Androctonus australis Beta-insect excitatory toxin 1 Proteins 0.000 description 1
- 108010037870 Anthranilate Synthase Proteins 0.000 description 1
- 108010039627 Aprotinin Proteins 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 235000011331 Brassica Nutrition 0.000 description 1
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 1
- 244000188595 Brassica sinapistrum Species 0.000 description 1
- 241000743778 Briza Species 0.000 description 1
- 102000000584 Calmodulin Human genes 0.000 description 1
- 108010041952 Calmodulin Proteins 0.000 description 1
- 108010080972 Catechol 2,3-dioxygenase Proteins 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 108010033170 Chloromuconate cycloisomerase Proteins 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
- 102000003712 Complement factor B Human genes 0.000 description 1
- 108090000056 Complement factor B Proteins 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 101710151559 Crystal protein Proteins 0.000 description 1
- 108010025880 Cyclomaltodextrin glucanotransferase Proteins 0.000 description 1
- 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 description 1
- 102000003849 Cytochrome P450 Human genes 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 101150048270 DHPS gene Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 108010028143 Dioxygenases Proteins 0.000 description 1
- 102000016680 Dioxygenases Human genes 0.000 description 1
- 102000010911 Enzyme Precursors Human genes 0.000 description 1
- 108010062466 Enzyme Precursors Proteins 0.000 description 1
- 241000588698 Erwinia Species 0.000 description 1
- 241000702191 Escherichia virus P1 Species 0.000 description 1
- 101710142246 External core antigen Proteins 0.000 description 1
- 108010001515 Galectin 4 Proteins 0.000 description 1
- 102100039556 Galectin-4 Human genes 0.000 description 1
- 108700023224 Glucose-1-phosphate adenylyltransferases Proteins 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 108010001483 Glycogen Synthase Proteins 0.000 description 1
- 108030006517 Glyphosate oxidoreductases Proteins 0.000 description 1
- 244000299507 Gossypium hirsutum Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 101001052477 Homo sapiens Mitogen-activated protein kinase 4 Proteins 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 102000004286 Hydroxymethylglutaryl CoA Reductases Human genes 0.000 description 1
- 108090000895 Hydroxymethylglutaryl CoA Reductases Proteins 0.000 description 1
- 102000009617 Inorganic Pyrophosphatase Human genes 0.000 description 1
- 108010009595 Inorganic Pyrophosphatase Proteins 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 108010042889 Inulosucrase Proteins 0.000 description 1
- 108010036940 Levansucrase Proteins 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- 241000709757 Luteovirus Species 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- JEVVKJMRZMXFBT-XWDZUXABSA-N Lycophyll Natural products OC/C(=C/CC/C(=C\C=C\C(=C/C=C/C(=C\C=C\C=C(/C=C/C=C(\C=C\C=C(/CC/C=C(/CO)\C)\C)/C)\C)/C)\C)/C)/C JEVVKJMRZMXFBT-XWDZUXABSA-N 0.000 description 1
- 241000723994 Maize dwarf mosaic virus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102100024189 Mitogen-activated protein kinase 4 Human genes 0.000 description 1
- 108010085220 Multiprotein Complexes Proteins 0.000 description 1
- 102000007474 Multiprotein Complexes Human genes 0.000 description 1
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 1
- 101710202365 Napin Proteins 0.000 description 1
- 241000207746 Nicotiana benthamiana Species 0.000 description 1
- 101710089395 Oleosin Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 101710149663 Osmotin Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000015731 Peptide Hormones Human genes 0.000 description 1
- 108010038988 Peptide Hormones Proteins 0.000 description 1
- 101710173432 Phytoene synthase Proteins 0.000 description 1
- 208000020584 Polyploidy Diseases 0.000 description 1
- 102000017033 Porins Human genes 0.000 description 1
- 108010013381 Porins Proteins 0.000 description 1
- 241000709769 Potato leafroll virus Species 0.000 description 1
- 241000709992 Potato virus X Species 0.000 description 1
- 241000710078 Potyvirus Species 0.000 description 1
- 101710184309 Probable sucrose-6-phosphate hydrolase Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 108010078762 Protein Precursors Proteins 0.000 description 1
- 102000014961 Protein Precursors Human genes 0.000 description 1
- 108010026552 Proteome Proteins 0.000 description 1
- 108010055016 Rec A Recombinases Proteins 0.000 description 1
- 102000001218 Rec A Recombinases Human genes 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 108700008530 Rice yellow mottle virus P1 Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 101100191561 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PRP3 gene Proteins 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 244000082988 Secale cereale Species 0.000 description 1
- 235000007238 Secale cereale Nutrition 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 244000062793 Sorghum vulgare Species 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 241000701955 Streptomyces virus phiC31 Species 0.000 description 1
- 102400000472 Sucrase Human genes 0.000 description 1
- 108020000005 Sucrose phosphorylase Proteins 0.000 description 1
- 101710112652 Sucrose-6-phosphate hydrolase Proteins 0.000 description 1
- 108700006291 Sucrose-phosphate synthases Proteins 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 108091008874 T cell receptors Proteins 0.000 description 1
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 description 1
- 241000710145 Tomato bushy stunt virus Species 0.000 description 1
- 102000009206 Translocator proteins Human genes 0.000 description 1
- 108050000091 Translocator proteins Proteins 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 102000018690 Trypsinogen Human genes 0.000 description 1
- 108010027252 Trypsinogen Proteins 0.000 description 1
- 102000003431 Ubiquitin-Conjugating Enzyme Human genes 0.000 description 1
- 108060008747 Ubiquitin-Conjugating Enzyme Proteins 0.000 description 1
- 244000025271 Umbellularia californica Species 0.000 description 1
- 235000008674 Umbellularia californica Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 241000235033 Zygosaccharomyces rouxii Species 0.000 description 1
- 108091000039 acetoacetyl-CoA reductase Proteins 0.000 description 1
- 108020002494 acetyltransferase Proteins 0.000 description 1
- 102000005421 acetyltransferase Human genes 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 108010050516 adenylate isopentenyltransferase Proteins 0.000 description 1
- 108010073338 aminoglycoside N(6')-acetyltransferase Proteins 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 229960004405 aprotinin Drugs 0.000 description 1
- 101150037081 aroA gene Proteins 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 229940019700 blood coagulation factors Drugs 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000004900 c-terminal fragment Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- WBYWAXJHAXSJNI-UHFFFAOYSA-N cinnamic acid Chemical compound OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000003527 fibrinolytic agent Substances 0.000 description 1
- 230000003480 fibrinolytic effect Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000030414 genetic transfer Effects 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 101150019926 glgA gene Proteins 0.000 description 1
- 101150065899 glgA1 gene Proteins 0.000 description 1
- 101150037310 glgM gene Proteins 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 108010002685 hygromycin-B kinase Proteins 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 description 1
- 229940047124 interferons Drugs 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 101150112190 luxD gene Proteins 0.000 description 1
- OAIJSZIZWZSQBC-GYZMGTAESA-N lycopene Chemical compound CC(C)=CCC\C(C)=C\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C=C(/C)CCC=C(C)C OAIJSZIZWZSQBC-GYZMGTAESA-N 0.000 description 1
- 229960004999 lycopene Drugs 0.000 description 1
- 235000012661 lycopene Nutrition 0.000 description 1
- 239000001751 lycopene Substances 0.000 description 1
- 230000002132 lysosomal effect Effects 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 108091005573 modified proteins Proteins 0.000 description 1
- 102000035118 modified proteins Human genes 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 210000004898 n-terminal fragment Anatomy 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- YCIMNLLNPGFGHC-UHFFFAOYSA-N o-dihydroxy-benzene Natural products OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 108020004410 pectinesterase Proteins 0.000 description 1
- 239000000813 peptide hormone Substances 0.000 description 1
- 238000002823 phage display Methods 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 229930029653 phosphoenolpyruvate Natural products 0.000 description 1
- DTBNBXWJWCWCIK-UHFFFAOYSA-N phosphoenolpyruvic acid Chemical compound OC(=O)C(=C)OP(O)(O)=O DTBNBXWJWCWCIK-UHFFFAOYSA-N 0.000 description 1
- 230000000865 phosphorylative effect Effects 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 229940085127 phytase Drugs 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000001323 posttranslational effect Effects 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 235000013930 proline Nutrition 0.000 description 1
- 150000003148 prolines Chemical class 0.000 description 1
- 239000013636 protein dimer Substances 0.000 description 1
- 230000006916 protein interaction Effects 0.000 description 1
- 230000005892 protein maturation Effects 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 230000012743 protein tagging Effects 0.000 description 1
- 231100000654 protein toxin Toxicity 0.000 description 1
- 230000004850 protein–protein interaction Effects 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000008521 reorganization Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 230000010153 self-pollination Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 108010076424 stilbene synthase Proteins 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229940124530 sulfonamide Drugs 0.000 description 1
- 150000003456 sulfonamides Chemical class 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000892 thaumatin Substances 0.000 description 1
- 235000010436 thaumatin Nutrition 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- ZCIHMQAPACOQHT-ZGMPDRQDSA-N trans-isorenieratene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/c1c(C)ccc(C)c1C)C=CC=C(/C)C=Cc2c(C)ccc(C)c2C ZCIHMQAPACOQHT-ZGMPDRQDSA-N 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000010474 transient expression Effects 0.000 description 1
- 108010020589 trehalose-6-phosphate synthase Proteins 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000001086 yeast two-hybrid system Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/8265—Transgene containment, e.g. gene dispersal
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
- C12N15/8289—Male sterility
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2003118644 DE10318644A1 (de) | 2002-05-31 | 2003-04-24 | Transgene Pflanzen mit kontrollierter Weitergabe eines Merkmals an die Nachkommen |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10224214 | 2002-05-31 | ||
| DE10224980 | 2002-06-05 | ||
| DE2003118644 DE10318644A1 (de) | 2002-05-31 | 2003-04-24 | Transgene Pflanzen mit kontrollierter Weitergabe eines Merkmals an die Nachkommen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE10318644A1 true DE10318644A1 (de) | 2003-12-24 |
Family
ID=29713122
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2003118644 Withdrawn DE10318644A1 (de) | 2002-05-31 | 2003-04-24 | Transgene Pflanzen mit kontrollierter Weitergabe eines Merkmals an die Nachkommen |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US7642404B2 (enExample) |
| EP (1) | EP1509610B1 (enExample) |
| JP (1) | JP4603882B2 (enExample) |
| AU (1) | AU2003226693B2 (enExample) |
| CA (1) | CA2483145C (enExample) |
| DE (1) | DE10318644A1 (enExample) |
| DK (1) | DK1509610T3 (enExample) |
| ES (1) | ES2388522T3 (enExample) |
| MX (1) | MXPA04010443A (enExample) |
| WO (1) | WO2003102197A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102012003848A1 (de) * | 2012-02-29 | 2013-08-29 | Kws Saat Ag | Pathogenresistente transgene Pflanze |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10325814A1 (de) * | 2003-06-06 | 2004-12-23 | Icon Genetics Ag | Sichere Erzeugung eines gewünschten Produkts in hybriden Samen |
| US9012729B2 (en) | 2008-06-03 | 2015-04-21 | Nordsaat Saatzuchtgesellschaft Mbh | Process of producing male sterile monocotyledonous plants |
| EP2516651A1 (en) * | 2009-12-22 | 2012-10-31 | Bayer CropScience NV | Herbicide tolerant plants |
| EP2423316B1 (en) * | 2010-08-25 | 2016-11-16 | Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) | Method for determining meiotic recombination frequencies in plants |
| KR101622942B1 (ko) | 2011-06-21 | 2016-05-20 | 서울대학교산학협력단 | 종자 발아 및 염색질의 구조 조절에 관여하는 유전자 및 이의 용도 |
| WO2013078433A1 (en) | 2011-11-23 | 2013-05-30 | University Of Hawaii | Auto-processing domains for polypeptide expression |
| WO2013138354A1 (en) | 2012-03-13 | 2013-09-19 | Pioneer Hi-Bred International, Inc. | Genetic reduction of male fertility in plants |
| WO2013138358A1 (en) | 2012-03-13 | 2013-09-19 | Pioneer Hi-Bred International, Inc. | Genetic reduction of male fertility in plants |
| EP2825550A2 (en) | 2012-03-13 | 2015-01-21 | Pioneer Hi-Bred International Inc. | Genetic reduction of male fertility in plants |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ241310A (en) | 1991-01-17 | 1995-03-28 | Gen Hospital Corp | Trans-splicing ribozymes |
| US5717129A (en) * | 1995-02-16 | 1998-02-10 | Pioneer Hi-Bred International, Inc. | Methods for maintaining sterility in plants |
| US6392119B1 (en) * | 1997-01-24 | 2002-05-21 | Dna Plant Technology Corporation | Two component plant cell lethality methods and compositions |
| AU3391900A (en) * | 1999-03-05 | 2000-09-21 | Maxygen, Inc. | Encryption of traits using split gene sequences |
| CN1676606A (zh) * | 1999-05-24 | 2005-10-05 | 新英格兰生物实验室公司 | 在植物中从蛋白片段重构靶蛋白的方法 |
| US5990395A (en) * | 1999-05-24 | 1999-11-23 | Novartis Ag | Inbred sweet corn line W1498A |
| US6852911B1 (en) * | 1999-08-31 | 2005-02-08 | Fertiseed Ltd. | Method of producing a male sterile plant by exogenic allelism |
| EP1255846B1 (en) * | 2000-02-11 | 2010-01-06 | Metabolix, Inc. | Multi-gene expression constructs containing modified inteins |
| EP1499734B1 (en) | 2002-04-29 | 2009-07-29 | Icon Genetics GmbH | Process of producing environmentally safe transgenic organisms |
-
2003
- 2003-03-21 EP EP03755923A patent/EP1509610B1/en not_active Expired - Lifetime
- 2003-03-21 ES ES03755923T patent/ES2388522T3/es not_active Expired - Lifetime
- 2003-03-21 WO PCT/EP2003/002986 patent/WO2003102197A1/en not_active Ceased
- 2003-03-21 JP JP2004510434A patent/JP4603882B2/ja not_active Expired - Fee Related
- 2003-03-21 CA CA2483145A patent/CA2483145C/en not_active Expired - Fee Related
- 2003-03-21 US US10/514,905 patent/US7642404B2/en not_active Expired - Fee Related
- 2003-03-21 DK DK03755923.4T patent/DK1509610T3/da active
- 2003-03-21 AU AU2003226693A patent/AU2003226693B2/en not_active Ceased
- 2003-03-21 MX MXPA04010443A patent/MXPA04010443A/es active IP Right Grant
- 2003-04-24 DE DE2003118644 patent/DE10318644A1/de not_active Withdrawn
-
2009
- 2009-11-19 US US12/621,765 patent/US8193410B2/en not_active Expired - Fee Related
-
2012
- 2012-05-14 US US13/470,812 patent/US8604281B2/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102012003848A1 (de) * | 2012-02-29 | 2013-08-29 | Kws Saat Ag | Pathogenresistente transgene Pflanze |
| EP2820137A1 (de) * | 2012-02-29 | 2015-01-07 | KWS Saat AG | Pathogenresistente transgene pflanze |
Also Published As
| Publication number | Publication date |
|---|---|
| US20130024986A1 (en) | 2013-01-24 |
| MXPA04010443A (es) | 2005-10-26 |
| US8193410B2 (en) | 2012-06-05 |
| US8604281B2 (en) | 2013-12-10 |
| WO2003102197A1 (en) | 2003-12-11 |
| EP1509610A1 (en) | 2005-03-02 |
| EP1509610B1 (en) | 2012-05-23 |
| DK1509610T3 (da) | 2012-09-03 |
| JP2005536990A (ja) | 2005-12-08 |
| WO2003102197A8 (en) | 2004-10-21 |
| AU2003226693A1 (en) | 2003-12-19 |
| US20100138948A1 (en) | 2010-06-03 |
| US20050172353A1 (en) | 2005-08-04 |
| CA2483145A1 (en) | 2003-12-11 |
| US7642404B2 (en) | 2010-01-05 |
| JP4603882B2 (ja) | 2010-12-22 |
| AU2003226693B2 (en) | 2008-02-21 |
| CA2483145C (en) | 2013-05-28 |
| ES2388522T3 (es) | 2012-10-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69033142T2 (de) | Ortsspezifische rekombination von dns in pflanzenzellen | |
| DE69534503T2 (de) | Kontrolle der pflanzengenexpression | |
| DE69636225T2 (de) | Stimulierung der homologen rekombination in pflanzlichen organismen mittels rekombinations fördernder enzyme | |
| US8604281B2 (en) | Transgenic plants with controlled distribution of a trait to progeny | |
| US11814632B2 (en) | Modified excisable MON87701 soybean transgenic insect resistance locus | |
| CA2354195A1 (en) | Methods and genetic compositions to limit outcrossing and undesired gene flow in crop plants | |
| DE102015006335A1 (de) | Verfahren und Konstrukte zur gezielten Nukleinsäure Editierung in Pflanzen | |
| DE60037200T2 (de) | Orts-spezifisches rekombinationssystem zur manipulation des plastidengenoms in höheren pflanzen | |
| Schiermeyer et al. | Targeted insertion of large DNA sequences by homology‐directed repair or non‐homologous end joining in engineered tobacco BY‐2 cells using designed zinc finger nucleases | |
| DE10132780A1 (de) | Plastidäre Genexpression über autonom replizierende Vektoren | |
| DE69835812T2 (de) | Anwendungsmoeglichkeiten der maennlichen sterilitaet von pflanzen | |
| US20210123067A1 (en) | Compositions and methods for transferring cytoplasmic or nuclear traits or components | |
| WO2019234129A1 (en) | Haploid induction with modified dna-repair | |
| DE10131690A1 (de) | Verfahren zum kontrollierten Umordnen von Chromosomenfragmenten für die Pflanzenzucht | |
| DE102015014252A1 (de) | Verfahren und Konstrukte zur gezielten Nukleinsäure Editierung in Pflanzen | |
| Kundu | Transgenic breeding | |
| JP2001514856A (ja) | 植物における遺伝子の選択的発現 | |
| US20200377909A1 (en) | Directed genome engineering using enhanced targeted editing technologies | |
| JP4518741B2 (ja) | 環境に安全なトランスジェニック生物を産生する方法 | |
| US20260071228A1 (en) | Guide polynucleotide multiplexing | |
| CN115243711B (zh) | 两步基因交换 | |
| Narkhed et al. | Ereeding | |
| BREEDING | RECENT ADVANCES IN TRANSGENIC BREEDING | |
| DE10303937A1 (de) | Pflanzentransformation mit Assemblierung einer gewünschten Sequenz in Vivo | |
| DE10332597A1 (de) | Transformation von Pflanzen unter Zusammenbau eines Merkmals in Vivo |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8141 | Disposal/no request for examination |