DE102004022263A1 - Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen - Google Patents
Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen Download PDFInfo
- Publication number
- DE102004022263A1 DE102004022263A1 DE102004022263A DE102004022263A DE102004022263A1 DE 102004022263 A1 DE102004022263 A1 DE 102004022263A1 DE 102004022263 A DE102004022263 A DE 102004022263A DE 102004022263 A DE102004022263 A DE 102004022263A DE 102004022263 A1 DE102004022263 A1 DE 102004022263A1
- Authority
- DE
- Germany
- Prior art keywords
- microarray
- detection
- probe
- reaction
- reaction chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 129
- 230000004001 molecular interaction Effects 0.000 title claims abstract description 17
- 238000001514 detection method Methods 0.000 claims abstract description 190
- 238000006243 chemical reaction Methods 0.000 claims abstract description 183
- 239000000523 sample Substances 0.000 claims abstract description 164
- 238000002493 microarray Methods 0.000 claims abstract description 149
- 230000003993 interaction Effects 0.000 claims abstract description 59
- 239000000758 substrate Substances 0.000 claims abstract description 52
- 150000007523 nucleic acids Chemical class 0.000 claims description 119
- 102000039446 nucleic acids Human genes 0.000 claims description 118
- 108020004707 nucleic acids Proteins 0.000 claims description 117
- 230000003321 amplification Effects 0.000 claims description 60
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 60
- 239000012488 sample solution Substances 0.000 claims description 30
- 230000003287 optical effect Effects 0.000 claims description 29
- 239000000126 substance Substances 0.000 claims description 27
- 238000012545 processing Methods 0.000 claims description 26
- 125000004122 cyclic group Chemical group 0.000 claims description 13
- 239000011521 glass Substances 0.000 claims description 13
- 239000003550 marker Substances 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 12
- 108090000623 proteins and genes Proteins 0.000 claims description 12
- 125000006850 spacer group Chemical group 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 12
- 230000002829 reductive effect Effects 0.000 claims description 11
- 239000011159 matrix material Substances 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 9
- 239000004033 plastic Substances 0.000 claims description 9
- 229920003023 plastic Polymers 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 7
- 238000010626 work up procedure Methods 0.000 claims description 7
- 229920001222 biopolymer Polymers 0.000 claims description 6
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 6
- 241001631457 Cannula Species 0.000 claims description 5
- 230000001276 controlling effect Effects 0.000 claims description 5
- 230000033228 biological regulation Effects 0.000 claims description 4
- 150000001720 carbohydrates Chemical class 0.000 claims description 4
- 235000014633 carbohydrates Nutrition 0.000 claims description 4
- 230000000670 limiting effect Effects 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000011068 loading method Methods 0.000 claims description 3
- 239000000427 antigen Substances 0.000 claims description 2
- 108091007433 antigens Proteins 0.000 claims description 2
- 102000036639 antigens Human genes 0.000 claims description 2
- 229920001577 copolymer Polymers 0.000 claims description 2
- 238000006557 surface reaction Methods 0.000 claims description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 91
- 238000009396 hybridization Methods 0.000 description 86
- 230000008569 process Effects 0.000 description 54
- 102000053602 DNA Human genes 0.000 description 51
- 108020004414 DNA Proteins 0.000 description 50
- 239000000243 solution Substances 0.000 description 33
- 238000010438 heat treatment Methods 0.000 description 31
- 238000000018 DNA microarray Methods 0.000 description 25
- 239000012528 membrane Substances 0.000 description 23
- 230000015572 biosynthetic process Effects 0.000 description 21
- 238000005755 formation reaction Methods 0.000 description 20
- 239000007788 liquid Substances 0.000 description 20
- 238000005259 measurement Methods 0.000 description 19
- 238000003491 array Methods 0.000 description 18
- 238000004458 analytical method Methods 0.000 description 17
- 238000001816 cooling Methods 0.000 description 17
- 239000000047 product Substances 0.000 description 17
- 108091034117 Oligonucleotide Proteins 0.000 description 15
- 230000027455 binding Effects 0.000 description 15
- 229920002477 rna polymer Polymers 0.000 description 15
- 238000000137 annealing Methods 0.000 description 14
- 230000000295 complement effect Effects 0.000 description 13
- 238000001556 precipitation Methods 0.000 description 13
- 238000007789 sealing Methods 0.000 description 13
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 12
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 12
- 239000002244 precipitate Substances 0.000 description 10
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 9
- 239000012491 analyte Substances 0.000 description 9
- 230000008901 benefit Effects 0.000 description 9
- 239000003068 molecular probe Substances 0.000 description 9
- 239000002853 nucleic acid probe Substances 0.000 description 9
- 238000005406 washing Methods 0.000 description 9
- 238000004925 denaturation Methods 0.000 description 8
- 230000036425 denaturation Effects 0.000 description 8
- 238000001917 fluorescence detection Methods 0.000 description 8
- 230000002441 reversible effect Effects 0.000 description 8
- 238000012408 PCR amplification Methods 0.000 description 7
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 7
- 230000008859 change Effects 0.000 description 7
- 238000013461 design Methods 0.000 description 7
- 238000006073 displacement reaction Methods 0.000 description 7
- 239000002773 nucleotide Substances 0.000 description 7
- 125000003729 nucleotide group Chemical group 0.000 description 7
- 229910052710 silicon Inorganic materials 0.000 description 7
- 239000010703 silicon Substances 0.000 description 7
- 238000013019 agitation Methods 0.000 description 6
- 239000000975 dye Substances 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 5
- 108020004635 Complementary DNA Proteins 0.000 description 5
- 238000010804 cDNA synthesis Methods 0.000 description 5
- 239000000919 ceramic Substances 0.000 description 5
- 239000002299 complementary DNA Substances 0.000 description 5
- 238000007906 compression Methods 0.000 description 5
- 230000006835 compression Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 230000005284 excitation Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 241000894007 species Species 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 4
- 229910010293 ceramic material Inorganic materials 0.000 description 4
- 230000008878 coupling Effects 0.000 description 4
- 238000010168 coupling process Methods 0.000 description 4
- 238000005859 coupling reaction Methods 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 230000001419 dependent effect Effects 0.000 description 4
- 239000013013 elastic material Substances 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- 238000003384 imaging method Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000003647 oxidation Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 4
- 229920000515 polycarbonate Polymers 0.000 description 4
- 239000004926 polymethyl methacrylate Substances 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 230000003068 static effect Effects 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 3
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 3
- 108091093037 Peptide nucleic acid Proteins 0.000 description 3
- 102000003992 Peroxidases Human genes 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 3
- 108010006785 Taq Polymerase Proteins 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000012512 characterization method Methods 0.000 description 3
- 239000012295 chemical reaction liquid Substances 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 229910052747 lanthanoid Inorganic materials 0.000 description 3
- 150000002602 lanthanoids Chemical class 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000004417 polycarbonate Substances 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 229920002223 polystyrene Polymers 0.000 description 3
- 238000003825 pressing Methods 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 230000005855 radiation Effects 0.000 description 3
- 230000002285 radioactive effect Effects 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- WKBPZYKAUNRMKP-UHFFFAOYSA-N 1-[2-(2,4-dichlorophenyl)pentyl]1,2,4-triazole Chemical compound C=1C=C(Cl)C=C(Cl)C=1C(CCC)CN1C=NC=N1 WKBPZYKAUNRMKP-UHFFFAOYSA-N 0.000 description 2
- 108020004491 Antisense DNA Proteins 0.000 description 2
- 229910001369 Brass Inorganic materials 0.000 description 2
- 206010013710 Drug interaction Diseases 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 229910018487 Ni—Cr Inorganic materials 0.000 description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- -1 antibodies Proteins 0.000 description 2
- 239000003816 antisense DNA Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000009529 body temperature measurement Methods 0.000 description 2
- 239000010951 brass Substances 0.000 description 2
- VNNRSPGTAMTISX-UHFFFAOYSA-N chromium nickel Chemical compound [Cr].[Ni] VNNRSPGTAMTISX-UHFFFAOYSA-N 0.000 description 2
- 239000004020 conductor Substances 0.000 description 2
- 238000001218 confocal laser scanning microscopy Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000002405 diagnostic procedure Methods 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 238000002073 fluorescence micrograph Methods 0.000 description 2
- 238000000799 fluorescence microscopy Methods 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 238000003505 heat denaturation Methods 0.000 description 2
- 238000013537 high throughput screening Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 238000007403 mPCR Methods 0.000 description 2
- 150000002739 metals Chemical class 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000005580 one pot reaction Methods 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 2
- 230000010287 polarization Effects 0.000 description 2
- 229920006254 polymer film Polymers 0.000 description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
- 150000005839 radical cations Chemical class 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 230000002787 reinforcement Effects 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 108020004418 ribosomal RNA Proteins 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000004065 semiconductor Substances 0.000 description 2
- 150000004756 silanes Chemical class 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 125000001424 substituent group Chemical group 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 239000010409 thin film Substances 0.000 description 2
- 239000011031 topaz Substances 0.000 description 2
- 229910052853 topaz Inorganic materials 0.000 description 2
- 238000000492 total internal reflection fluorescence microscopy Methods 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 1
- 108091023037 Aptamer Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108091028026 C-DNA Proteins 0.000 description 1
- 229910004261 CaF 2 Inorganic materials 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 108700020911 DNA-Binding Proteins Proteins 0.000 description 1
- 108010043461 Deep Vent DNA polymerase Proteins 0.000 description 1
- QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 1
- 229910052693 Europium Inorganic materials 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 239000004425 Makrolon Substances 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108010067902 Peptide Library Proteins 0.000 description 1
- 108700020962 Peroxidase Proteins 0.000 description 1
- 108010002747 Pfu DNA polymerase Proteins 0.000 description 1
- 102000044126 RNA-Binding Proteins Human genes 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 229910052772 Samarium Inorganic materials 0.000 description 1
- 229910004298 SiO 2 Inorganic materials 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 101000865057 Thermococcus litoralis DNA polymerase Proteins 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 229910052769 Ytterbium Inorganic materials 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 101150010487 are gene Proteins 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000005415 bioluminescence Methods 0.000 description 1
- 230000029918 bioluminescence Effects 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 108010064866 biozym Proteins 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000002788 crimping Methods 0.000 description 1
- 230000002559 cytogenic effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000000994 depressogenic effect Effects 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 229960000633 dextran sulfate Drugs 0.000 description 1
- 239000005546 dideoxynucleotide Substances 0.000 description 1
- KZNICNPSHKQLFF-UHFFFAOYSA-N dihydromaleimide Natural products O=C1CCC(=O)N1 KZNICNPSHKQLFF-UHFFFAOYSA-N 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 238000005370 electroosmosis Methods 0.000 description 1
- 150000002118 epoxides Chemical class 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000010408 film Substances 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000002875 fluorescence polarization Methods 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- ZNKMCMOJCDFGFT-UHFFFAOYSA-N gold titanium Chemical compound [Ti].[Au] ZNKMCMOJCDFGFT-UHFFFAOYSA-N 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 150000002463 imidates Chemical class 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 229940127121 immunoconjugate Drugs 0.000 description 1
- 238000003365 immunocytochemistry Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 150000002540 isothiocyanates Chemical class 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000002991 molded plastic Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 238000012634 optical imaging Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 238000002161 passivation Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920000447 polyanionic polymer Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000001454 recorded image Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000012958 reprocessing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- KZUNJOHGWZRPMI-UHFFFAOYSA-N samarium atom Chemical compound [Sm] KZUNJOHGWZRPMI-UHFFFAOYSA-N 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229910052594 sapphire Inorganic materials 0.000 description 1
- 239000010980 sapphire Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 239000004054 semiconductor nanocrystal Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 108700014590 single-stranded DNA binding proteins Proteins 0.000 description 1
- 108091000371 single-stranded RNA binding proteins Proteins 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229960002317 succinimide Drugs 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 239000013077 target material Substances 0.000 description 1
- 238000005496 tempering Methods 0.000 description 1
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 1
- 229910001258 titanium gold Inorganic materials 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- NAWDYIZEMPQZHO-UHFFFAOYSA-N ytterbium Chemical compound [Yb] NAWDYIZEMPQZHO-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0654—Lenses; Optical fibres
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0877—Flow chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1838—Means for temperature control using fluid heat transfer medium
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/02—Mechanical
- G01N2201/024—Modular construction
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Dispersion Chemistry (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Optics & Photonics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Length Measuring Devices With Unspecified Measuring Means (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Priority Applications (28)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102004022263A DE102004022263A1 (de) | 2004-05-06 | 2004-05-06 | Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen |
| EP10180859.0A EP2299257B1 (fr) | 2004-05-06 | 2005-05-06 | Dispositif et procédé pour détecter des interactions moléculaires |
| DK05739547.7T DK1761641T3 (da) | 2004-05-06 | 2005-05-06 | Indretning og fremgangsmåde til påvisning af molekylære vekselvirkninger |
| BRPI0510680A BRPI0510680B8 (pt) | 2004-05-06 | 2005-05-06 | dispositivo e método para detecção qualitativa e/ou quantitativa de interações moleculares entre moléculas de sonda e moléculas alvo, e, uso de um dispositivo |
| EP05739547A EP1761641B1 (fr) | 2004-05-06 | 2005-05-06 | Dispositif et procede pour de detecter des interactions moleculaires |
| CA3041596A CA3041596A1 (fr) | 2004-05-06 | 2005-05-06 | Dispositif et procede pour de detecter des interactions |
| CN2010105855578A CN102121054B (zh) | 2004-05-06 | 2005-05-06 | 用于检测分子相互作用的装置和方法 |
| EP10172432.6A EP2256478B1 (fr) | 2004-05-06 | 2005-05-06 | Dispositif et procede pour de detecter des interactions moleculaires |
| EP10180895.4A EP2290351B1 (fr) | 2004-05-06 | 2005-05-06 | Dispositif et procede pour de detecter des interactions moleculaires |
| DE502005010078T DE502005010078D1 (de) | 2004-05-06 | 2005-05-06 | Vorrichtung und verfahren zum nachweis von molekularen wechselwirkungen |
| PT10180859T PT2299257T (pt) | 2004-05-06 | 2005-05-06 | Dispositivo e método para detecção de interacções moleculares |
| AU2005240757A AU2005240757B2 (en) | 2004-05-06 | 2005-05-06 | Device and method for detecting molecular interactions |
| EP16204878.9A EP3171155A1 (fr) | 2004-05-06 | 2005-05-06 | Dispositif et procede pour de detecter des interactions moleculaires |
| EP18189028.6A EP3527287A1 (fr) | 2004-05-06 | 2005-05-06 | Procéde pour de détecter des interactions moléculaires |
| ES05739547T ES2350638T3 (es) | 2004-05-06 | 2005-05-06 | Dispositivos y procedimientos para la detección de interacciones moleculares. |
| AT05739547T ATE477338T1 (de) | 2004-05-06 | 2005-05-06 | Vorrichtung und verfahren zum nachweis von molekularen wechselwirkungen |
| CA2565679A CA2565679C (fr) | 2004-05-06 | 2005-05-06 | Dispositif et procede pour de detecter des interactions moleculaires |
| CN2005800143517A CN1981188B (zh) | 2004-05-06 | 2005-05-06 | 用于检测分子相互作用的装置和方法 |
| CN2010105825290A CN102127595B (zh) | 2004-05-06 | 2005-05-06 | 用于检测分子相互作用的装置和方法 |
| PCT/EP2005/004923 WO2005108604A2 (fr) | 2004-05-06 | 2005-05-06 | Dispositif et procede pour de detecter des interactions moleculaires |
| JP2007512036A JP4958770B2 (ja) | 2004-05-06 | 2005-05-06 | 分子相互作用を検出する装置および方法 |
| NZ551229A NZ551229A (en) | 2004-05-06 | 2005-05-06 | Device and method for detecting molecular interactions |
| EP10180879.8A EP2280267B1 (fr) | 2004-05-06 | 2005-05-06 | Dispositif et procédé pour de detecter des interactions moleculaires |
| US11/593,021 US8916348B2 (en) | 2004-05-06 | 2006-11-06 | Method and device for the detection of molecular interactions |
| HK07106972.2A HK1102610A1 (en) | 2004-05-06 | 2007-06-29 | Device and method for detecting molecular interactions |
| AU2011201671A AU2011201671B2 (en) | 2004-05-06 | 2011-04-14 | Device and method for detecting molecular interactions |
| US14/580,137 US20160237482A1 (en) | 2004-05-06 | 2014-12-22 | Method and device for the detection of molecular interactions |
| US17/154,749 US20210172010A1 (en) | 2004-05-06 | 2021-01-21 | Method and device for the detection of molecular interactions |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102004022263A DE102004022263A1 (de) | 2004-05-06 | 2004-05-06 | Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE102004022263A1 true DE102004022263A1 (de) | 2005-12-15 |
Family
ID=35169865
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE102004022263A Pending DE102004022263A1 (de) | 2004-05-06 | 2004-05-06 | Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen |
| DE502005010078T Active DE502005010078D1 (de) | 2004-05-06 | 2005-05-06 | Vorrichtung und verfahren zum nachweis von molekularen wechselwirkungen |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE502005010078T Active DE502005010078D1 (de) | 2004-05-06 | 2005-05-06 | Vorrichtung und verfahren zum nachweis von molekularen wechselwirkungen |
Country Status (14)
| Country | Link |
|---|---|
| EP (7) | EP2280267B1 (fr) |
| JP (1) | JP4958770B2 (fr) |
| CN (3) | CN102121054B (fr) |
| AT (1) | ATE477338T1 (fr) |
| AU (2) | AU2005240757B2 (fr) |
| BR (1) | BRPI0510680B8 (fr) |
| CA (2) | CA3041596A1 (fr) |
| DE (2) | DE102004022263A1 (fr) |
| DK (1) | DK1761641T3 (fr) |
| ES (1) | ES2350638T3 (fr) |
| HK (1) | HK1102610A1 (fr) |
| NZ (1) | NZ551229A (fr) |
| PT (1) | PT2299257T (fr) |
| WO (1) | WO2005108604A2 (fr) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008000770A1 (fr) * | 2006-06-27 | 2008-01-03 | Zenteris Gmbh | Chambre de réaction chauffable pour traiter une biopuce et procédé pour actionner une telle chambre de réaction |
| WO2008046930A1 (fr) * | 2006-10-20 | 2008-04-24 | Clondiag Gmbh | Dispositifs et méthodes de dosage destinés à la détection de substances à analyser |
| DE102007052281A1 (de) | 2007-11-02 | 2009-05-07 | Zenteris Gmbh | Einschritt-Multiplex-Immuntest |
| EP2458382A1 (fr) | 2006-11-22 | 2012-05-30 | CLONDIAG GmbH | Dispositif pour analyse comprenant plusieurs zones de test, méthode utilisant le dispositif |
| DE102014018535A1 (de) * | 2014-12-12 | 2016-06-16 | Nanotemper Technologies Gmbh | System und Verfahren für ein abdichtungsfreies Temperieren von Kapillaren |
| US9925536B2 (en) | 2007-07-23 | 2018-03-27 | Clondiag Gmbh | Assays for measuring nucleic acids |
Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102005052713A1 (de) * | 2005-11-04 | 2007-05-16 | Clondiag Chip Tech Gmbh | Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen |
| EP1758981A4 (fr) | 2004-05-28 | 2013-01-16 | Wafergen Inc | Appareil et procedes pour effectuer des analyses multiplex |
| JP4705504B2 (ja) * | 2006-04-05 | 2011-06-22 | アイダエンジニアリング株式会社 | マイクロ流体チップ |
| US10753927B2 (en) | 2006-09-22 | 2020-08-25 | ALERE TECHNOLOGIES GmbH | Methods for detecting an analyte |
| EP2644272B1 (fr) * | 2006-11-06 | 2016-04-20 | CLONDIAG GmbH | Dosages |
| US9097671B2 (en) | 2006-11-22 | 2015-08-04 | Clondiag Gmbh | Assays |
| DE102006056540A1 (de) * | 2006-11-28 | 2008-05-29 | Zenteris Gmbh | Vorrichtung und Verfahren zur Untersuchung von biologischen und medizinischen Proben |
| EP2109627A4 (fr) | 2007-01-22 | 2014-07-23 | Wafergen Inc | Appareil permettant la mise en oeuvre de réactions chimiques à haut rendement |
| US8633013B2 (en) | 2007-05-03 | 2014-01-21 | Clondiag Gmbh | Assays |
| CA2705355C (fr) * | 2007-11-13 | 2013-02-26 | F. Hoffmann-La Roche Ag | Cassette de detection modulaire |
| EP2227537A4 (fr) | 2007-12-24 | 2014-05-07 | Honeywell Int Inc | Réacteur pour l'analyse quantitative d'acides nucléiques |
| JP2009168508A (ja) * | 2008-01-11 | 2009-07-30 | Toppan Printing Co Ltd | Ｄｎａチップ装置、携帯端末装置、及び情報連絡システム |
| DE102008054313B4 (de) | 2008-11-03 | 2012-12-13 | Zenteris Gmbh | Kartusche und Vorrichtung zur Untersuchung biologischer Proben mit temperaturgesteuerten biologischen Reaktionen |
| WO2010088496A1 (fr) * | 2009-01-30 | 2010-08-05 | Gen-Probe Incorporated | Systèmes et procédés de détection d'un signal et d'application d'énergie thermique à un élément de transmission de signal |
| US20140152801A1 (en) | 2009-10-28 | 2014-06-05 | Alentic Microscience Inc. | Detecting and Using Light Representative of a Sample |
| US9539571B2 (en) | 2010-01-20 | 2017-01-10 | Honeywell International Inc. | Method to increase detection efficiency of real time PCR microarray by quartz material |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| JP5893607B2 (ja) | 2010-04-05 | 2016-03-23 | プログノシス バイオサイエンシズ インコーポレイテッドＰｒｏｇｎｏｓｙｓ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． | 空間コード化生物学的アッセイ |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| JP2011250714A (ja) * | 2010-05-31 | 2011-12-15 | Sanyo Electric Co Ltd | 増幅装置、検出装置 |
| WO2012072795A2 (fr) | 2010-12-03 | 2012-06-07 | ALERE TECHNOLOGIES GmbH | Transformation d'un matériau en un état à modulation optique au moyen d'un rayonnement laser |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| JP6340364B2 (ja) | 2012-05-23 | 2018-06-06 | アルベルト−ルートヴィヒ−ウニベルシタット フライブルク | 分子の集積をリアルタイムに検出する及び／又は分子マイクロアレイの作製プロセスをモニタリングする装置及び方法 |
| US10502666B2 (en) | 2013-02-06 | 2019-12-10 | Alentic Microscience Inc. | Sample processing improvements for quantitative microscopy |
| US9518920B2 (en) | 2013-06-26 | 2016-12-13 | Alentic Microscience Inc. | Sample processing improvements for microscopy |
| WO2014210223A1 (fr) | 2013-06-25 | 2014-12-31 | Prognosys Biosciences, Inc. | Essais biologiques à codage spatial faisant appel à un dispositif microfluidique |
| GB201403076D0 (en) | 2014-02-21 | 2014-04-09 | ALERE TECHNOLOGIES GmbH | Methods for detecting multiple nucleic acids in a sample |
| WO2016132738A1 (fr) * | 2015-02-19 | 2016-08-25 | パナソニックＩｐマネジメント株式会社 | Cellule de détection et dispositif de détection l'utilisant |
| US10641772B2 (en) | 2015-02-20 | 2020-05-05 | Takara Bio Usa, Inc. | Method for rapid accurate dispensing, visualization and analysis of single cells |
| ES2935860T3 (es) | 2015-04-10 | 2023-03-13 | Spatial Transcriptomics Ab | Análisis de ácidos nucleicos múltiplex, espacialmente distinguidos de especímenes biológicos |
| CN105842234B (zh) * | 2016-05-11 | 2018-10-02 | 四川理工学院 | 自标定式二氧化氮连续在线检测装置及检测方法 |
| CN105738354B (zh) * | 2016-05-11 | 2018-10-02 | 四川理工学院 | 气液相化学发光检测装置及检测方法 |
| WO2017220483A1 (fr) * | 2016-06-21 | 2017-12-28 | Koninklijke Philips N.V. | Système et procédé de détection d'un analyte |
| US11460405B2 (en) | 2016-07-21 | 2022-10-04 | Takara Bio Usa, Inc. | Multi-Z imaging and dispensing with multi-well devices |
| EP3744429A1 (fr) | 2016-10-07 | 2020-12-02 | Boehringer Ingelheim Vetmedica GmbH | Système d'analyse et procédé d'examen d'un échantillon |
| CN108690875A (zh) * | 2017-04-05 | 2018-10-23 | 杭州丹威生物科技有限公司 | 用来筛查传染病基因和遗传改变的带有条码的微阵列芯片及使用方法 |
| IL253067B (en) | 2017-06-21 | 2022-01-01 | Gyntools Ltd | Test devices and test fixture for use with them |
| US11346850B2 (en) | 2017-06-21 | 2022-05-31 | Gyntools Ltd | Assay system including assay apparatus and handheld single use assay devices for use therewith |
| DE102017218849A1 (de) * | 2017-10-23 | 2019-04-25 | Robert Bosch Gmbh | Reaktionsträger für eine mikrofluidische Vorrichtung und Verfahren zur Bestimmung einer Nukleotidsequenz |
| EP3560593B1 (fr) | 2018-04-25 | 2024-06-05 | OPTOLANE Technologies Inc. | Cartouche de pcr numérique en temps réel |
| CN108896495B (zh) * | 2018-09-14 | 2024-04-12 | 贵州电网有限责任公司 | 一种环保绝缘气体与金属相容性试验模拟装置及试验方法 |
| EP3894585A2 (fr) | 2018-12-10 | 2021-10-20 | 10X Genomics, Inc. | Génération de sondes de capture pour analyse spatiale |
| CN110161003B (zh) * | 2019-05-17 | 2022-07-22 | 深圳市刚竹医疗科技有限公司 | 光学检测装置及实时荧光定量核酸扩增检测系统 |
| IL267301A (en) | 2019-06-12 | 2019-11-28 | Gyntools Ltd | A test device and hand-held sample collection tools for it |
| IL292168B2 (en) | 2019-10-30 | 2024-05-01 | Gyntools Ltd | A test system includes a test fixture and disposable hand-held test devices for use therewith |
| US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
| CN111220588B (zh) * | 2020-03-24 | 2023-05-16 | 哈尔滨工业大学（威海） | 一种基于油膜荧光亮度的流场辐聚辐散测量方法 |
| EP4153775A1 (fr) | 2020-05-22 | 2023-03-29 | 10X Genomics, Inc. | Mesure spatio-temporelle simultanée de l'expression génique et de l'activité cellulaire |
| EP4162074B1 (fr) | 2020-06-08 | 2024-04-24 | 10X Genomics, Inc. | Méthodes de détermination de marge chirurgicale et méthodes d'utilisation associées |
| WO2022140028A1 (fr) | 2020-12-21 | 2022-06-30 | 10X Genomics, Inc. | Procédés, compositions et systèmes pour capturer des sondes et/ou des codes à barres |
| CN113514415B (zh) * | 2021-04-25 | 2023-03-10 | 中国科学技术大学 | 一种基于红外光谱成像的液态样品的分子间相互作用表征方法 |
| WO2024126241A1 (fr) * | 2022-12-12 | 2024-06-20 | Robert Bosch Gmbh | Dispositif microfluidique, en particulier cartouche, avec un tampon pour le transfert de chaleur vers un substrat d'analyse |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5324633A (en) * | 1991-11-22 | 1994-06-28 | Affymax Technologies N.V. | Method and apparatus for measuring binding affinity |
| DE19711281C1 (de) * | 1997-03-18 | 1998-04-16 | Inst Chemo Biosensorik | Vorrichtung und Verfahren zur Durchführung von Fluoreszenzimmunotests |
| US5834758A (en) * | 1994-09-02 | 1998-11-10 | Affymetrix, Inc. | Method and apparatus for imaging a sample on a device |
| EP1108472A2 (fr) * | 1999-12-15 | 2001-06-20 | Hitachi, Ltd. | Puce à ADN améliorée à gradient thermiques (ATGC), son procédé de fabrication et méthode pour la réalisation de réactions biochimiques |
| EP1132485A2 (fr) * | 2000-03-10 | 2001-09-12 | Hitachi Software Engineering Co., Ltd. | Procédé et appareil pour détecter des réactions d'hybridisation |
| DE10027524A1 (de) * | 2000-06-02 | 2001-12-13 | Max Planck Gesellschaft | Vorrichtung und Verfahren zur Bearbeitung von substratgebundenen Proben |
| DE19941905C2 (de) * | 1999-09-02 | 2002-06-06 | Max Planck Gesellschaft | Probenkammer zur Flüssigkeitsbehandlung biologischer Proben |
| US20020127709A1 (en) * | 1998-12-07 | 2002-09-12 | Wheatcroft Roger George Laurence | Rotary thermocycling apparatus |
| US6471916B1 (en) * | 1999-11-09 | 2002-10-29 | Packard Instrument Company | Apparatus and method for calibration of a microarray scanning system |
| US20040043479A1 (en) * | 2000-12-11 | 2004-03-04 | Briscoe Cynthia G. | Multilayerd microfluidic devices for analyte reactions |
| WO2004029195A2 (fr) * | 2002-09-24 | 2004-04-08 | U.S. Government As Represented By The Secretary Of The Army | Thermocycleur portatif |
| EP1431749A1 (fr) * | 2001-09-28 | 2004-06-23 | Hitachi, Ltd. | Dispositif de detection de luminescence et plaque de jeux ordonnes de microechantillons |
Family Cites Families (50)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2035058A1 (fr) * | 1969-03-19 | 1970-12-18 | American Optical Corp | |
| US4038030A (en) * | 1975-04-10 | 1977-07-26 | American Hospital Supply Corporation | Profile analysis pack and method |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US5013646A (en) | 1989-11-03 | 1991-05-07 | Transgenic Sciences, Inc. | TMB Formulation for soluble and precipitable HRP-ELISA |
| GB9014263D0 (en) | 1990-06-27 | 1990-08-15 | Dixon Arthur E | Apparatus and method for spatially- and spectrally- resolvedmeasurements |
| GB9015793D0 (en) | 1990-07-18 | 1990-09-05 | Medical Res Council | Confocal scanning optical microscope |
| US5412087A (en) | 1992-04-24 | 1995-05-02 | Affymax Technologies N.V. | Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces |
| US5364790A (en) * | 1993-02-16 | 1994-11-15 | The Perkin-Elmer Corporation | In situ PCR amplification system |
| US6027880A (en) | 1995-08-02 | 2000-02-22 | Affymetrix, Inc. | Arrays of nucleic acid probes and methods of using the same for detecting cystic fibrosis |
| US5459325A (en) | 1994-07-19 | 1995-10-17 | Molecular Dynamics, Inc. | High-speed fluorescence scanner |
| US5510621A (en) * | 1994-10-03 | 1996-04-23 | Optical Solutions, Inc. | Apparatus and method for measuring components in a bag |
| AU5171696A (en) | 1995-02-27 | 1996-09-18 | Ely Michael Rabani | Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism |
| US5585639A (en) | 1995-07-27 | 1996-12-17 | Hewlett-Packard Company | Optical scanning apparatus |
| US5863502A (en) * | 1996-01-24 | 1999-01-26 | Sarnoff Corporation | Parallel reaction cassette and associated devices |
| CA2255774C (fr) | 1996-05-29 | 2008-03-18 | Cornell Research Foundation, Inc. | Detection de differences dans des sequences d'acides nucleiques utilisant une combinaison de la detection par ligase et de reactions d'amplification en chaine par polymerase |
| US5760950A (en) | 1996-07-25 | 1998-06-02 | Advanced Scanning, Ltd. | Scanning confocal microscope |
| DE19706570C1 (de) | 1997-02-19 | 1998-02-26 | Inst Physikalische Hochtech Ev | Verfahren zur Herstellung von strukturierten, selbstorganisierten molekularen Monolagen einzelner molekularer Spezies, insbesondere von Substanzbibliotheken |
| DE19725050C2 (de) | 1997-06-13 | 1999-06-24 | Fraunhofer Ges Forschung | Anordnung zur Detektion biochemischer oder chemischer Substanzen mittels Fluoreszenzlichtanregung und Verfahren zu deren Herstellung |
| US6043506A (en) * | 1997-08-13 | 2000-03-28 | Bio-Rad Laboratories, Inc. | Multi parameter scanner |
| WO2000005336A2 (fr) * | 1998-07-22 | 2000-02-03 | Ljl Biosystems, Inc. | Dispositifs et procedes d'analyse d'echantillons |
| US6197503B1 (en) | 1997-11-26 | 2001-03-06 | Ut-Battelle, Llc | Integrated circuit biochip microsystem containing lens |
| CA2632856C (fr) * | 1998-06-24 | 2010-10-12 | Chen & Chen, Llc | Dispositif d'analyse d'echantillons de fluide |
| WO2000014281A2 (fr) * | 1998-08-21 | 2000-03-16 | Naxcor | Dosages utilisant des acides nucleiques reticulables immobilises |
| US6271042B1 (en) | 1998-08-26 | 2001-08-07 | Alpha Innotech Corporation | Biochip detection system |
| AU5855699A (en) | 1998-08-28 | 2000-03-21 | Jerini Biotools Gmbh | Method for producing polymeric solid phase supporting materials |
| AT410718B (de) | 1998-10-28 | 2003-07-25 | Schindler Hansgeorg Dr | Vorrichtung zur visualisierung von molekülen |
| US6948843B2 (en) * | 1998-10-28 | 2005-09-27 | Covaris, Inc. | Method and apparatus for acoustically controlling liquid solutions in microfluidic devices |
| CN100533146C (zh) | 1999-05-19 | 2009-08-26 | 埃佩多夫阵列技术股份有限公司 | 鉴定和/或定量靶化合物的方法 |
| AU768113B2 (en) | 1999-07-02 | 2003-12-04 | Clondiag Chip Technologies Gmbh | Microchip matrix device for duplicating and characterizing nucleic acids |
| WO2001027327A2 (fr) * | 1999-10-08 | 2001-04-19 | Protogene Laboratories, Inc. | Procede et appareil destines a produire un grand nombre de reactions au moyen d'une plaque matrice |
| US6569674B1 (en) * | 1999-12-15 | 2003-05-27 | Amersham Biosciences Ab | Method and apparatus for performing biological reactions on a substrate surface |
| US6589778B1 (en) * | 1999-12-15 | 2003-07-08 | Amersham Biosciences Ab | Method and apparatus for performing biological reactions on a substrate surface |
| JP2003517156A (ja) * | 1999-12-15 | 2003-05-20 | モトローラ・インコーポレイテッド | 生物学的反応を行う組成物および方法 |
| WO2002002810A2 (fr) | 2000-07-01 | 2002-01-10 | Clondiag Chip Technologies Gmbh | Procede de detection qualitative et/ou quantitative d'interactions moleculaires sur des microplaques |
| DE10058394C1 (de) * | 2000-11-24 | 2002-07-11 | Siemens Ag | Verfahren für die biochemische Analytik und zugehörige Anordnung |
| US6905816B2 (en) * | 2000-11-27 | 2005-06-14 | Intelligent Medical Devices, Inc. | Clinically intelligent diagnostic devices and methods |
| CN2473211Y (zh) * | 2001-02-05 | 2002-01-23 | 徐荣臻 | 一种蛋白芯片反应器 |
| EP1372848A4 (fr) * | 2001-03-09 | 2006-08-09 | Biomicro Systems Inc | Procede et systeme d'interfa age microfluidique avec des reseaux |
| US7201833B2 (en) * | 2001-06-04 | 2007-04-10 | Epocal Inc. | Integrated solid-phase hydrophilic matrix circuits and micro-arrays |
| JP4244534B2 (ja) * | 2001-06-12 | 2009-03-25 | 横河電機株式会社 | バイオチップ |
| DE10132785A1 (de) | 2001-07-06 | 2003-01-16 | Clondiag Chip Tech Gmbh | Verfahren zum Nachweis von in einer Polymerase-Kettenreaktion amplifizierten Nukleinsäuremolekülen |
| DE10142643A1 (de) | 2001-08-31 | 2003-04-24 | Clondiag Chip Tech Gmbh | Detektion von Wechselwirkungen auf Sonden-Arrays |
| WO2003022435A2 (fr) * | 2001-09-11 | 2003-03-20 | Iquum, Inc. | Tubes echantillons |
| KR100442822B1 (ko) * | 2001-10-23 | 2004-08-02 | 삼성전자주식회사 | 전단응력 측정을 이용한 생분자들간의 결합 여부 검출 방법 |
| ATE408455T1 (de) * | 2001-12-19 | 2008-10-15 | Affymetrix Inc | Arrayplatten und verfahren zur herstellung von arrayplatten |
| CN1202263C (zh) * | 2002-02-25 | 2005-05-18 | 财团法人工业技术研究院 | 高效核酸杂交装置及方法 |
| EP1345026B1 (fr) * | 2002-03-15 | 2010-05-05 | Affymetrix, Inc. | Système et methode de balayage de materiaux biologiques |
| JP2004003888A (ja) * | 2002-05-31 | 2004-01-08 | Olympus Corp | 生体関連物質の検査装置とその反応ステージ |
| DE10253966B4 (de) | 2002-11-19 | 2005-03-24 | Clondiag Chip Technologies Gmbh | Microarray-basiertes Verfahren zur Amplifikation und Detektion von Nukleinsäuren in einem kontinuierlichen Prozess |
| DE10315074A1 (de) | 2003-04-02 | 2004-10-14 | Clondiag Chip Technologies Gmbh | Vorrichtung zur Vervielfältigung und zum Nachweis von Nukleinsäuren |
-
2004
- 2004-05-06 DE DE102004022263A patent/DE102004022263A1/de active Pending
-
2005
- 2005-05-06 ES ES05739547T patent/ES2350638T3/es active Active
- 2005-05-06 JP JP2007512036A patent/JP4958770B2/ja active Active
- 2005-05-06 EP EP10180879.8A patent/EP2280267B1/fr active Active
- 2005-05-06 EP EP05739547A patent/EP1761641B1/fr active Active
- 2005-05-06 PT PT10180859T patent/PT2299257T/pt unknown
- 2005-05-06 CN CN2010105855578A patent/CN102121054B/zh active Active
- 2005-05-06 DE DE502005010078T patent/DE502005010078D1/de active Active
- 2005-05-06 CA CA3041596A patent/CA3041596A1/fr not_active Abandoned
- 2005-05-06 CN CN2010105825290A patent/CN102127595B/zh active Active
- 2005-05-06 EP EP10180859.0A patent/EP2299257B1/fr active Active
- 2005-05-06 EP EP10180895.4A patent/EP2290351B1/fr active Active
- 2005-05-06 EP EP16204878.9A patent/EP3171155A1/fr not_active Withdrawn
- 2005-05-06 CN CN2005800143517A patent/CN1981188B/zh active Active
- 2005-05-06 WO PCT/EP2005/004923 patent/WO2005108604A2/fr active Application Filing
- 2005-05-06 EP EP10172432.6A patent/EP2256478B1/fr active Active
- 2005-05-06 DK DK05739547.7T patent/DK1761641T3/da active
- 2005-05-06 NZ NZ551229A patent/NZ551229A/en unknown
- 2005-05-06 AT AT05739547T patent/ATE477338T1/de active
- 2005-05-06 EP EP18189028.6A patent/EP3527287A1/fr active Pending
- 2005-05-06 CA CA2565679A patent/CA2565679C/fr active Active
- 2005-05-06 AU AU2005240757A patent/AU2005240757B2/en active Active
- 2005-05-06 BR BRPI0510680A patent/BRPI0510680B8/pt active IP Right Grant
-
2007
- 2007-06-29 HK HK07106972.2A patent/HK1102610A1/xx unknown
-
2011
- 2011-04-14 AU AU2011201671A patent/AU2011201671B2/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5324633A (en) * | 1991-11-22 | 1994-06-28 | Affymax Technologies N.V. | Method and apparatus for measuring binding affinity |
| US5834758A (en) * | 1994-09-02 | 1998-11-10 | Affymetrix, Inc. | Method and apparatus for imaging a sample on a device |
| DE19711281C1 (de) * | 1997-03-18 | 1998-04-16 | Inst Chemo Biosensorik | Vorrichtung und Verfahren zur Durchführung von Fluoreszenzimmunotests |
| US20020127709A1 (en) * | 1998-12-07 | 2002-09-12 | Wheatcroft Roger George Laurence | Rotary thermocycling apparatus |
| DE19941905C2 (de) * | 1999-09-02 | 2002-06-06 | Max Planck Gesellschaft | Probenkammer zur Flüssigkeitsbehandlung biologischer Proben |
| US6471916B1 (en) * | 1999-11-09 | 2002-10-29 | Packard Instrument Company | Apparatus and method for calibration of a microarray scanning system |
| EP1108472A2 (fr) * | 1999-12-15 | 2001-06-20 | Hitachi, Ltd. | Puce à ADN améliorée à gradient thermiques (ATGC), son procédé de fabrication et méthode pour la réalisation de réactions biochimiques |
| EP1132485A2 (fr) * | 2000-03-10 | 2001-09-12 | Hitachi Software Engineering Co., Ltd. | Procédé et appareil pour détecter des réactions d'hybridisation |
| DE10027524A1 (de) * | 2000-06-02 | 2001-12-13 | Max Planck Gesellschaft | Vorrichtung und Verfahren zur Bearbeitung von substratgebundenen Proben |
| US20040043479A1 (en) * | 2000-12-11 | 2004-03-04 | Briscoe Cynthia G. | Multilayerd microfluidic devices for analyte reactions |
| EP1431749A1 (fr) * | 2001-09-28 | 2004-06-23 | Hitachi, Ltd. | Dispositif de detection de luminescence et plaque de jeux ordonnes de microechantillons |
| WO2004029195A2 (fr) * | 2002-09-24 | 2004-04-08 | U.S. Government As Represented By The Secretary Of The Army | Thermocycleur portatif |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8926922B2 (en) | 2006-06-27 | 2015-01-06 | Zenteris Gmbh | Heated reaction chamber for processing a biochip and method for controlling said reaction chamber |
| WO2008000767A1 (fr) * | 2006-06-27 | 2008-01-03 | Zenteris Gmbh | Dispositif de refroidissement pour chambre de réaction utilisée pour traiter une biopuce et procédé pour actionner un tel dispositif de refroidissement |
| WO2008000770A1 (fr) * | 2006-06-27 | 2008-01-03 | Zenteris Gmbh | Chambre de réaction chauffable pour traiter une biopuce et procédé pour actionner une telle chambre de réaction |
| US8151589B2 (en) | 2006-06-27 | 2012-04-10 | Zenteris, GmbH | Cooling device for a reaction chamber for processing a biochip and method for controlling said cooling device |
| DE112007001596B4 (de) * | 2006-06-27 | 2010-05-12 | Zenteris Gmbh | Beheizbare Reaktionskammer zum Prozessieren eines Biochips |
| US8110157B2 (en) | 2006-06-27 | 2012-02-07 | Zenteris Gmbh | Heated reaction chamber for processing a biochip and method for controlling said reaction chamber |
| EP2548647A1 (fr) * | 2006-10-20 | 2013-01-23 | CLONDIAG GmbH | Dispositifs et méthodes de dosage destinés à la détection de substances á analyser |
| WO2008046930A1 (fr) * | 2006-10-20 | 2008-04-24 | Clondiag Gmbh | Dispositifs et méthodes de dosage destinés à la détection de substances à analyser |
| EP2458382A1 (fr) | 2006-11-22 | 2012-05-30 | CLONDIAG GmbH | Dispositif pour analyse comprenant plusieurs zones de test, méthode utilisant le dispositif |
| US9925536B2 (en) | 2007-07-23 | 2018-03-27 | Clondiag Gmbh | Assays for measuring nucleic acids |
| EP3527288A1 (fr) | 2007-07-23 | 2019-08-21 | Alere Technologies GmbH | Procédé de détection de virus |
| DE102007052281A1 (de) | 2007-11-02 | 2009-05-07 | Zenteris Gmbh | Einschritt-Multiplex-Immuntest |
| DE102014018535A1 (de) * | 2014-12-12 | 2016-06-16 | Nanotemper Technologies Gmbh | System und Verfahren für ein abdichtungsfreies Temperieren von Kapillaren |
| EP3229966A1 (fr) * | 2014-12-12 | 2017-10-18 | NanoTemper Technologies GmbH | Procédé et système de thermorégulation de capillaires exempts d'étanchéité |
| US10618051B2 (en) | 2014-12-12 | 2020-04-14 | Nanotemper Technologies Gmbh | Method and system for tempering capillaries without sealing them |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2290351B1 (fr) | Dispositif et procede pour de detecter des interactions moleculaires | |
| DE102005052752A1 (de) | Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen | |
| EP2266699B1 (fr) | Appareil pour l'amplification et la détection d'acide nucléique | |
| DE10201463B4 (de) | Reaktionsgefäß zur Durchführung von Array-Verfahren | |
| EP1943360B1 (fr) | Procede et dispositif de detection d'interactions moleculaires | |
| EP2809803B1 (fr) | Pcr numérique multiplexée | |
| US20160237482A1 (en) | Method and device for the detection of molecular interactions | |
| DE102007031137A1 (de) | Verfahren und Sonden/Primärsystem zum "real time" Nachweis eines Nukleinsäuretargets |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| R082 | Change of representative |
Representative=s name: MAIWALD GMBH, DE Representative=s name: MAIWALD PATENTANWALTS- UND RECHTSANWALTSGESELL, DE |
|
| R002 | Refusal decision in examination/registration proceedings |