CY1120531T1 - Βιοελεγχος - Google Patents

Βιοελεγχος

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Publication number
CY1120531T1
CY1120531T1 CY20181100819T CY181100819T CY1120531T1 CY 1120531 T1 CY1120531 T1 CY 1120531T1 CY 20181100819 T CY20181100819 T CY 20181100819T CY 181100819 T CY181100819 T CY 181100819T CY 1120531 T1 CY1120531 T1 CY 1120531T1
Authority
CY
Cyprus
Prior art keywords
expression
transcription
gene
promoter
upstream flow
Prior art date
Application number
CY20181100819T
Other languages
English (en)
Inventor
Luke Alphey
Original Assignee
Oxitec Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxitec Limited filed Critical Oxitec Limited
Publication of CY1120531T1 publication Critical patent/CY1120531T1/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
    • A01K67/0337—Genetically modified Arthropods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
    • A01K67/0337—Genetically modified Arthropods
    • A01K67/0339—Genetically modified insects, e.g. Drosophila melanogaster, medfly
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M99/00—Subject matter not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/052—Animals comprising random inserted nucleic acids (transgenic) inducing gain of function
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A01K2217/203—Animal model comprising inducible/conditional expression system, e.g. hormones, tet
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A01K2217/206—Animal model comprising tissue-specific expression system, e.g. tissue specific expression of transgene, of Cre recombinase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A01K2227/706—Insects, e.g. Drosophila melanogaster, medfly
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/002—Vectors comprising a special translation-regulating system controllable or inducible

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Veterinary Medicine (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Παρέχεται ένα σύστημα έκφρασης γονιδίων βλαστικής γραμμής αρσενικών αρθροπόδων κατάλληλο για εξαρτημένη έκφραση ενός θηλαστικού γονιδίου σε μία βλαστική γραμμή αρσενικών Αρθροπόδων. Το σύστημα περιλαμβάνει μια πρώτη μονάδα έκφρασης που περιλαμβάνει ένα τελεστικό γονίδιο και έναν προαγωγό για αυτό συνδεδεμένο λειτουργικώς επ' αυτού• και μια δεύτερη μονάδα έκφρασης. Η εν λόγω δεύτερη μονάδα περιλαμβάνει μια αλληλουχία κωδίκευσης για έναν τελεστή μεταγραφής και ένα ρυθμιστικό στοιχείο ανοδικής ροής λειτουργικώς συνδεδεμένο επ' αυτής, ο δε τελεστής μεταγραφής όντας δυνάμενος δράσης επί του προαγωγού στην πρώτη μονάδα έκφρασης για καθοδήγηση έκφρασης του τελεστικού γονιδίου. Το ρυθμιστικό στοιχείο ανοδικής ροής συμπεριλαμβάνει έναν προαγωγό για τον τελεστή μεταγραφής• και ένα 5' UTR παρακείμενο σε ένα σημείο έναρξης για την αλληλουχία κωδίκευσης τελεστή μεταγραφής. Το ρυθμιστικό στοιχείο ανοδικής ροής που καθοδηγεί επαρκή έκφραση του τελεστή μεταγραφής ούτως ώστε η πρωτεΐνη του τελεστή μεταγραφής με τη σειρά να καθοδηγεί την μεταγραφή του τελεστικού γονιδίου προ της μείωσης. Επίσης παρέχονται και χρήσεις του συστήματος για την περίσταση σε μεθόδους βιοελέγχου και ελέγχου ποιότητας.
CY20181100819T 2012-03-05 2018-08-07 Βιοελεγχος CY1120531T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1203850.1A GB2500113A (en) 2012-03-05 2012-03-05 Arthropod male germline gene expression system
PCT/EP2013/054417 WO2013131920A1 (en) 2012-03-05 2013-03-05 Biocontrol

Publications (1)

Publication Number Publication Date
CY1120531T1 true CY1120531T1 (el) 2019-07-10

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Family Applications (1)

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CY20181100819T CY1120531T1 (el) 2012-03-05 2018-08-07 Βιοελεγχος

Country Status (23)

Country Link
US (2) US9487801B2 (el)
EP (2) EP2823047B1 (el)
JP (1) JP6363023B2 (el)
CN (2) CN104271747B (el)
AR (1) AR091314A1 (el)
AU (2) AU2013229578B2 (el)
BR (1) BR112014021922B1 (el)
CA (1) CA2866411A1 (el)
CL (1) CL2014002369A1 (el)
CO (1) CO7160024A2 (el)
CR (1) CR20140439A (el)
CY (1) CY1120531T1 (el)
ES (1) ES2683039T3 (el)
GB (1) GB2500113A (el)
GT (1) GT201400188A (el)
MA (1) MA37380B1 (el)
MX (1) MX359468B (el)
MY (1) MY169450A (el)
PE (1) PE20142278A1 (el)
PH (1) PH12014501993A1 (el)
SG (2) SG11201405481SA (el)
WO (1) WO2013131920A1 (el)
ZA (1) ZA201406925B (el)

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Also Published As

Publication number Publication date
GB201203850D0 (en) 2012-04-18
AU2013229578B2 (en) 2017-07-20
GB2500113A (en) 2013-09-11
PE20142278A1 (es) 2015-01-15
BR112014021922B1 (pt) 2022-06-28
CR20140439A (es) 2015-01-13
MA37380B1 (fr) 2017-01-31
WO2013131920A1 (en) 2013-09-12
PH12014501993B1 (en) 2014-11-24
JP2015516800A (ja) 2015-06-18
CA2866411A1 (en) 2013-09-12
CN108018309A (zh) 2018-05-11
EP2823047A1 (en) 2015-01-14
MY169450A (en) 2019-04-11
SG10201702181PA (en) 2017-05-30
CN104271747A (zh) 2015-01-07
MA37380A1 (fr) 2016-05-31
CO7160024A2 (es) 2015-01-15
EP3395949A1 (en) 2018-10-31
ES2683039T3 (es) 2018-09-24
MX359468B (es) 2018-09-28
JP6363023B2 (ja) 2018-07-25
US20170009253A1 (en) 2017-01-12
SG11201405481SA (en) 2014-10-30
US9487801B2 (en) 2016-11-08
EP2823047B1 (en) 2018-05-09
MX2014010712A (es) 2015-03-10
ZA201406925B (en) 2022-05-25
CN104271747B (zh) 2017-11-14
AU2017248469A1 (en) 2017-11-02
AR091314A1 (es) 2015-01-28
AU2013229578A1 (en) 2014-10-23
GT201400188A (es) 2016-03-01
US20150143552A1 (en) 2015-05-21
CL2014002369A1 (es) 2015-02-27
PH12014501993A1 (en) 2014-11-24
BR112014021922A2 (pt) 2017-07-04

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