MX2016006816A - Promotores sinteticos para celulas de ovario de hamster chino (cho), metodos de produccion de promotores sinteticos usando modulos de sitio de union del factor de transcripcion. - Google Patents
Promotores sinteticos para celulas de ovario de hamster chino (cho), metodos de produccion de promotores sinteticos usando modulos de sitio de union del factor de transcripcion.Info
- Publication number
- MX2016006816A MX2016006816A MX2016006816A MX2016006816A MX2016006816A MX 2016006816 A MX2016006816 A MX 2016006816A MX 2016006816 A MX2016006816 A MX 2016006816A MX 2016006816 A MX2016006816 A MX 2016006816A MX 2016006816 A MX2016006816 A MX 2016006816A
- Authority
- MX
- Mexico
- Prior art keywords
- synthetic promoters
- methods
- binding site
- transcription factor
- cho cells
- Prior art date
Links
- 102000040945 Transcription factor Human genes 0.000 title 1
- 108091023040 Transcription factor Proteins 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
- 230000002103 transcriptional effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/24—Vectors characterised by the absence of particular element, e.g. selectable marker, viral origin of replication
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/40—Systems of functionally co-operating vectors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
Abstract
La presente invención se relaciona con construcciones del promotor sintético específico de la célula CHO para expresar proteínas recombinantes, una biblioteca de construcciones del promotor de las mismas y un método para producir las construcciones del promotor. Las construcciones del promotor permiten un control preciso de la transcripción del gen recombinante sobre tres órdenes de magnitud, con los promotores se expresión superior capaces de duplicar la actividad transcripcional del promotor de CMV.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB201321109A GB201321109D0 (en) | 2013-11-29 | 2013-11-29 | Synthetic promoters |
GB201411717A GB201411717D0 (en) | 2014-07-01 | 2014-07-01 | Synthetic promoters |
PCT/EP2014/076024 WO2015079053A2 (en) | 2013-11-29 | 2014-11-28 | Synthetic promoters |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2016006816A true MX2016006816A (es) | 2016-08-19 |
Family
ID=52002952
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2016006816A MX2016006816A (es) | 2013-11-29 | 2014-11-28 | Promotores sinteticos para celulas de ovario de hamster chino (cho), metodos de produccion de promotores sinteticos usando modulos de sitio de union del factor de transcripcion. |
Country Status (13)
Country | Link |
---|---|
US (1) | US20170002378A1 (es) |
EP (1) | EP3074516A2 (es) |
JP (1) | JP2016537987A (es) |
KR (1) | KR20160087903A (es) |
CN (1) | CN105793415A (es) |
AU (1) | AU2014356396A1 (es) |
BR (1) | BR112016012177A2 (es) |
CA (1) | CA2930344A1 (es) |
CL (1) | CL2016001195A1 (es) |
IL (1) | IL245565A0 (es) |
MX (1) | MX2016006816A (es) |
RU (1) | RU2016125744A (es) |
WO (1) | WO2015079053A2 (es) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3568474A1 (en) | 2017-01-10 | 2019-11-20 | Intrexon Corporation | Modulating expression of polypeptides via new gene switch expression systems |
CN110582574A (zh) * | 2017-03-13 | 2019-12-17 | 麻省理工学院 | 合成启动子 |
JP7200129B2 (ja) * | 2017-04-19 | 2023-01-06 | メディミューン リミテッド | ユーザー定義の機能性を有する哺乳動物プロモーターのインシリコ設計 |
KR20200132870A (ko) * | 2018-03-20 | 2020-11-25 | 지머젠 인코포레이티드 | 중국 햄스터 난소 세포의 유전자 조작을 위한 htp 플랫폼 |
CN110343699A (zh) * | 2018-04-08 | 2019-10-18 | 新乡医学院 | 用于提高哺乳动物细胞转基因表达的调控序列、表达载体、表达系统及其应用 |
JP2022538598A (ja) | 2019-06-25 | 2022-09-05 | ミガル ガリラヤ リサーチ インスティテュート リミテッド | プレmRNAのトランススプライシングに基づく合成転写用論理「AND」ゲートを作製するためのシステムおよびその使用 |
EP4136237A1 (en) * | 2020-04-15 | 2023-02-22 | Asklepios Biopharmaceutical, Inc. | Forskolin-inducible promoters and hypoxia-inducible promoters |
JP2021185829A (ja) * | 2020-05-29 | 2021-12-13 | 花王株式会社 | 新規プロモーター |
EP4308710A1 (en) * | 2021-03-16 | 2024-01-24 | Astrazeneca AB | Control of multi-gene expression using synthetic promoters |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000034435A2 (en) * | 1998-12-08 | 2000-06-15 | Clontech Laboratories, Inc. | Cis-element reporter constructs and uses thereof |
AU2003295366B2 (en) * | 2002-11-04 | 2011-11-24 | Advisys, Inc. | Synthetic muscle promoters with activities exceeding naturally occurring regulatory sequences in cardiac cells |
US7312202B2 (en) * | 2003-02-18 | 2007-12-25 | Board Of Regents, The University Of Texas System | Rationally designed and chemically synthesized promoter for genetic vaccine and gene therapy |
JP4430535B2 (ja) * | 2003-07-21 | 2010-03-10 | ロンザ・バイオロジクス・ピーエルシー | Cho細胞において組換えタンパク質を発現する方法 |
US20070020237A1 (en) * | 2005-06-01 | 2007-01-25 | Biotech Institute For International Innovation, Inc. | Glucose inducible insulin expression and methods of treating diabetes |
CN101134110B (zh) * | 2006-09-01 | 2012-11-07 | 中国医学科学院基础医学研究所 | Q型非病毒载体以及包含其的药物组合物 |
-
2014
- 2014-11-28 US US15/100,071 patent/US20170002378A1/en not_active Abandoned
- 2014-11-28 RU RU2016125744A patent/RU2016125744A/ru unknown
- 2014-11-28 EP EP14805889.4A patent/EP3074516A2/en not_active Withdrawn
- 2014-11-28 KR KR1020167017364A patent/KR20160087903A/ko not_active Application Discontinuation
- 2014-11-28 WO PCT/EP2014/076024 patent/WO2015079053A2/en active Application Filing
- 2014-11-28 CN CN201480064846.XA patent/CN105793415A/zh active Pending
- 2014-11-28 MX MX2016006816A patent/MX2016006816A/es unknown
- 2014-11-28 BR BR112016012177A patent/BR112016012177A2/pt not_active Application Discontinuation
- 2014-11-28 AU AU2014356396A patent/AU2014356396A1/en not_active Abandoned
- 2014-11-28 JP JP2016534943A patent/JP2016537987A/ja active Pending
- 2014-11-28 CA CA2930344A patent/CA2930344A1/en not_active Abandoned
-
2016
- 2016-05-10 IL IL245565A patent/IL245565A0/en unknown
- 2016-05-18 CL CL2016001195A patent/CL2016001195A1/es unknown
Also Published As
Publication number | Publication date |
---|---|
CA2930344A1 (en) | 2015-06-04 |
WO2015079053A2 (en) | 2015-06-04 |
BR112016012177A2 (pt) | 2017-09-26 |
IL245565A0 (en) | 2016-06-30 |
WO2015079053A3 (en) | 2015-10-08 |
CN105793415A (zh) | 2016-07-20 |
US20170002378A1 (en) | 2017-01-05 |
JP2016537987A (ja) | 2016-12-08 |
KR20160087903A (ko) | 2016-07-22 |
AU2014356396A1 (en) | 2016-06-02 |
EP3074516A2 (en) | 2016-10-05 |
RU2016125744A (ru) | 2018-01-09 |
CL2016001195A1 (es) | 2017-07-28 |
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