ES2683039T3 - Control biológico - Google Patents

Control biológico Download PDF

Info

Publication number
ES2683039T3
ES2683039T3 ES13711848T ES13711848T ES2683039T3 ES 2683039 T3 ES2683039 T3 ES 2683039T3 ES 13711848 T ES13711848 T ES 13711848T ES 13711848 T ES13711848 T ES 13711848T ES 2683039 T3 ES2683039 T3 ES 2683039T3
Authority
ES
Spain
Prior art keywords
transcription factor
expression
promoter
effector gene
regulatory element
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES13711848T
Other languages
English (en)
Inventor
Luke Alphey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxitec Ltd
Original Assignee
Oxitec Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxitec Ltd filed Critical Oxitec Ltd
Application granted granted Critical
Publication of ES2683039T3 publication Critical patent/ES2683039T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
    • A01K67/0337—Genetically modified Arthropods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
    • A01K67/0337—Genetically modified Arthropods
    • A01K67/0339—Genetically modified insects, e.g. Drosophila melanogaster, medfly
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M99/00—Subject matter not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/052—Animals comprising random inserted nucleic acids (transgenic) inducing gain of function
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A01K2217/203—Animal model comprising inducible/conditional expression system, e.g. hormones, tet
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A01K2217/206—Animal model comprising tissue-specific expression system, e.g. tissue specific expression of transgene, of Cre recombinase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A01K2227/706—Insects, e.g. Drosophila melanogaster, medfly
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/002—Vectors comprising a special translation-regulating system controllable or inducible

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Veterinary Medicine (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Sistema de expresión génica de la línea germinal masculina de artrópodo adecuado para la expresión condicional de un gen efector en una línea germinal masculina de artrópodo, comprendiendo el sistema: - una primera unidad de expresión comprendiendo un gen efector y un promotor para este ligado de forma funcional al mismo; - una segunda unidad de expresión comprendiendo una secuencia de codificación para un factor de transcripción y un elemento regulador en la dirección 5' ligado de forma funcional a este, siendo capaz el factor de transcripción de actuar sobre el promotor en la primera unidad de expresión para conducir la expresión del gen efector, incluyendo el elemento regulador en la dirección 5': - un promotor para el factor de transcripción, donde el promotor es de Beta-2 Tubulin (B2T), matotopetli (topi) o always early (aly); y - una 5'-UTR adyacente a un sitio de inicio para la secuencia de codificación del factor de transcripción, donde la 5'-UTR es de heat shock protein 83 (hsp83), aly, o topi; conduciendo el elemento regulador en la dirección 5' la suficiente expresión del factor de transcripción de tal forma que la proteína del factor de transcripción conduce a su vez la transcripción del gen efector antes de la meiosis.
ES13711848T 2012-03-05 2013-03-05 Control biológico Active ES2683039T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1203850.1A GB2500113A (en) 2012-03-05 2012-03-05 Arthropod male germline gene expression system
PCT/EP2013/054417 WO2013131920A1 (en) 2012-03-05 2013-03-05 Biocontrol

Publications (1)

Publication Number Publication Date
ES2683039T3 true ES2683039T3 (es) 2018-09-24

Family

ID=46003145

Family Applications (1)

Application Number Title Priority Date Filing Date
ES13711848T Active ES2683039T3 (es) 2012-03-05 2013-03-05 Control biológico

Country Status (23)

Country Link
US (2) US9487801B2 (es)
EP (2) EP3395949A1 (es)
JP (1) JP6363023B2 (es)
CN (2) CN104271747B (es)
AR (2) AR091314A1 (es)
AU (2) AU2013229578B2 (es)
BR (1) BR112014021922B1 (es)
CA (1) CA2866411A1 (es)
CL (1) CL2014002369A1 (es)
CO (1) CO7160024A2 (es)
CR (1) CR20140439A (es)
CY (1) CY1120531T1 (es)
ES (1) ES2683039T3 (es)
GB (1) GB2500113A (es)
GT (1) GT201400188A (es)
MA (1) MA37380B1 (es)
MX (1) MX359468B (es)
MY (1) MY169450A (es)
PE (1) PE20142278A1 (es)
PH (1) PH12014501993B1 (es)
SG (2) SG10201702181PA (es)
WO (1) WO2013131920A1 (es)
ZA (1) ZA201406925B (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2404382B (en) 2003-07-28 2008-01-30 Oxitec Ltd Pest control
GB2443186A (en) 2006-10-25 2008-04-30 Oxitec Ltd Expression system for mediating alternative splicing
GB201303932D0 (en) 2013-03-05 2013-04-17 Oxitec Ltd Muscle actin promoter
WO2015105928A1 (en) * 2014-01-08 2015-07-16 President And Fellows Of Harvard College Rna-guided gene drives
GB2526867A (en) 2014-06-05 2015-12-09 Oxitec Ltd Gene expression system
WO2018029534A1 (en) 2016-08-12 2018-02-15 Oxitec Ltd. A self-limiting, sex-specific gene and methods of using
GB201810253D0 (en) * 2018-06-22 2018-08-08 Imperial Innovations Ltd Gene drive
GB201810256D0 (en) * 2018-06-22 2018-08-08 Imperial Innovations Ltd Polynucleotide
US20210324409A1 (en) * 2018-08-14 2021-10-21 Oxitec Limited Self-Selecting Sterile Male Arthropods
CN109105341A (zh) * 2018-09-04 2019-01-01 安徽师范大学 一种用于防治斑翅果蝇的辐照不育方法
GB2579224A (en) * 2018-11-26 2020-06-17 Zyzzle Ltd Apparatus for producing an improved animal population
EP3660053A1 (en) * 2018-11-27 2020-06-03 Fraunhofer Gesellschaft zur Förderung der Angewand Multiprotein expression systems for pest control
WO2020178822A1 (en) * 2019-03-05 2020-09-10 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Genome-edited birds
CN114395018A (zh) * 2021-12-31 2022-04-26 天津大学 CifA和CifB突变蛋白用于调控胞质不相容性的用途

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5180873A (en) 1985-04-16 1993-01-19 Dna Plant Technology Corporation Transformation of plants to introduce closely linked markers
GB8901677D0 (en) 1989-01-26 1989-03-15 Ici Plc Hybrid seed production
US5643776A (en) 1988-11-01 1997-07-01 The Regents Of The University Of California Insect diagnostic and control compositions
US5254801A (en) 1990-12-03 1993-10-19 Monsanto Company Heterologous dominant conditional lethal gene which is a phosphonate monoester hydrolase and use thereof in plants
GB9216151D0 (en) 1992-07-29 1992-09-09 Ici Plc Containment of plant germplasm
US6225121B1 (en) 1992-09-14 2001-05-01 Institute Of Molecular Biology And Biotechnology/Forth Eukaryotic transposable element
GB9313975D0 (en) 1993-07-06 1993-08-18 Sandoz Ltd Improvements in or relating to organic compounds
EG23907A (en) 1994-08-01 2007-12-30 Delta & Pine Land Co Control of plant gene expression
US5670353A (en) 1994-08-25 1997-09-23 Mycogen Plant Science, Inc. Subgenomic promoter
US5753434A (en) 1995-02-10 1998-05-19 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for altering sexual behavior
US5851796A (en) 1995-06-07 1998-12-22 Yale University Autoregulatory tetracycline-regulated system for inducible gene expression in eucaryotes
FR2737731B1 (fr) 1995-08-07 1997-10-10 Pasteur Institut Sequence de retroelements naturels ou synthetiques ayant pour fonction de permettre l'insertion de sequences nucleotidiques dans une cellule eucaryote
AUPN816196A0 (en) 1996-02-19 1996-03-14 Forbio Research Pty Ltd Regulation of eukaryotic gene expression
US5773697A (en) 1996-04-23 1998-06-30 Pioneer Hi-Bred International, Inc. Genetic constructs and methods for producing fruits with very little or diminished seed
EP0920521A1 (en) 1996-08-26 1999-06-09 Genencor International, Inc. IDENTIFICATION OF AND CLONING A MOBILE TRANSPOSON FROM $i(ASPERGILLUS)
JP2001501824A (ja) * 1996-10-01 2001-02-13 ユニバーシティー オブ ジョージア リサーチ ファウンデーション，インコーポレイテッド 昆虫特異的ダニ毒素遺伝子を発現する生物学的昆虫防除剤、方法および組成物
US7135608B1 (en) 1997-08-28 2006-11-14 The Salk Institute For Biological Studies Site-specific recombination in eukaryotes and constructs useful therefor
US6338040B1 (en) 1999-02-12 2002-01-08 Agren, Inc. Method for delaying the development in pest species of resistance to control techniques, using insurance to encourage correct uses of refuges
AU5642800A (en) 1999-06-01 2000-12-18 Inserm Method of transposon-mediated mutagenesis in the nematode LTiGTcaenorhabditisLT/iGT LTiGTelegansLT/iGT
GB2355459B (en) 1999-11-29 2001-09-26 Isis Innovation A dominant conditional lethal genetic system
WO2001059088A2 (en) 2000-02-11 2001-08-16 Queen Mary & Westfield College Vector
US20030150007A1 (en) 2000-03-21 2003-08-07 Charalambos Savakis Method of generating transgenic organisms using transposons
US20060057553A1 (en) 2000-05-30 2006-03-16 Estuardo Aguilar-Cordova Chimeric viral vectors for gene therapy
US6962810B2 (en) 2000-10-31 2005-11-08 University Of Notre Dame Du Lac Methods and compositions for transposition using minimal segments of the eukaryotic transformation vector piggyBac
EP1339863A2 (en) 2000-12-05 2003-09-03 Wisconsin Alumni Research Foundation Double transposition methods for manipulating nucleic acids
ITRM20010120A1 (it) 2001-03-08 2002-09-08 Univ Napoli Federico Ii Gene cctra come strumento per produrre progenie di soli maschi nella mosca mediterranea ceratitis capitata.
FR2825579B1 (fr) 2001-06-11 2004-07-23 Rhobio Methode d'obtention de plante monocotyledone contenant un gene d'interet sans sequence auxiliaire etrangere
DE10251918A1 (de) 2002-11-08 2004-05-19 Horn, Carsten, Dipl.-Biochem. Dr. Systeme zur Erzeugung stabiler genomischer Transgen-Insertionen
GB2402675B (en) 2003-05-12 2008-02-20 Oxitec Ltd Resistance dilution
AU2004245598B2 (en) 2003-06-10 2009-01-08 National Research Council Of Canada A coumermycin/novobiocin-regulated gene expression system
GB2403475B (en) 2003-07-01 2008-02-06 Oxitec Ltd Stable integrands
GB2404382B (en) * 2003-07-28 2008-01-30 Oxitec Ltd Pest control
US7569362B2 (en) 2004-03-15 2009-08-04 Biogen Idec Ma Inc. Methods and constructs for expressing polypeptide multimers in eukaryotic cells using alternative splicing
US7563601B1 (en) 2005-06-01 2009-07-21 City Of Hope Artificial riboswitch for controlling pre-mRNA splicing
EP3159414A1 (en) 2006-02-10 2017-04-26 Oxitec Limited Expression system
GB2443186A (en) 2006-10-25 2008-04-30 Oxitec Ltd Expression system for mediating alternative splicing
JP2008067678A (ja) * 2006-09-15 2008-03-27 Kyushu Institute Of Technology 昆虫の減数分裂期における染色体標的組換え誘導方法
US8198079B2 (en) * 2007-04-26 2012-06-12 Merck Sharp & Dohme Corp. Synthetic expression vectors for insect cells
WO2009016627A1 (en) * 2007-07-30 2009-02-05 Yissum Research Development Company Of The Hebrew University Of Jerusalem Sex-peptides resistant to proteolytic degradation and their use in biological control of insects
US20110088105A1 (en) * 2008-03-20 2011-04-14 Wimmer Ernst A Development stage-specific lethality system for insect population control
KR20110029156A (ko) 2008-06-27 2011-03-22 메뤼스 베.페. 항체 생산 비-인간 포유동물
CN102939383B (zh) * 2009-12-30 2015-04-29 先锋国际良种公司 用于靶向多核苷酸修饰的方法和组合物

Also Published As

Publication number Publication date
PE20142278A1 (es) 2015-01-15
US9487801B2 (en) 2016-11-08
CN104271747B (zh) 2017-11-14
CR20140439A (es) 2015-01-13
GB201203850D0 (en) 2012-04-18
MX359468B (es) 2018-09-28
CY1120531T1 (el) 2019-07-10
GT201400188A (es) 2016-03-01
CN108018309A (zh) 2018-05-11
AR091314A1 (es) 2015-01-28
PH12014501993A1 (en) 2014-11-24
EP2823047A1 (en) 2015-01-14
CN104271747A (zh) 2015-01-07
MX2014010712A (es) 2015-03-10
US20170009253A1 (en) 2017-01-12
MA37380A1 (fr) 2016-05-31
AU2013229578A1 (en) 2014-10-23
GB2500113A (en) 2013-09-11
US20150143552A1 (en) 2015-05-21
JP6363023B2 (ja) 2018-07-25
AU2017248469A1 (en) 2017-11-02
EP2823047B1 (en) 2018-05-09
BR112014021922A2 (pt) 2017-07-04
ZA201406925B (en) 2022-05-25
AR128556A2 (es) 2024-05-22
BR112014021922B1 (pt) 2022-06-28
WO2013131920A1 (en) 2013-09-12
SG10201702181PA (en) 2017-05-30
CO7160024A2 (es) 2015-01-15
PH12014501993B1 (en) 2014-11-24
CA2866411A1 (en) 2013-09-12
MY169450A (en) 2019-04-11
EP3395949A1 (en) 2018-10-31
SG11201405481SA (en) 2014-10-30
MA37380B1 (fr) 2017-01-31
AU2013229578B2 (en) 2017-07-20
CL2014002369A1 (es) 2015-02-27
JP2015516800A (ja) 2015-06-18

Similar Documents

Publication Publication Date Title
ES2683039T3 (es) Control biológico
SG10201808940WA (en) Nox inhibitor and nfкb inhibitor containing methoxyflavone
EP3099299A4 (en) Benzoquinoline inhibitors of vesicular monoamine transporter 2
EP3655531A4 (en) SEQUENCING OF NUCLEIC ACIDS ASSOCIATED WITH EXOSOMAL ISOLATION IN PATIENTS WITH MULTIFORM GLIOBLASTOMA
GB2539981B (en) System to control and condition the supply of natural gas to bi-fuel engines
HRP20182116T1 (hr) Sustav za obradu balastne vode s jedinicom za obradu balastne vode u strojarnici broda s uronjivom crpkom
CO2020012691A2 (es) Sistema de tratamiento de agua
DK3532059T3 (da) Kombinationsterapi med bromdomæne- og ekstraterminal proteinhæmmer
SG11201501548WA (en) Fuel gas supply system and method of ship
AR120721A2 (es) Péptidos y composiciones para el tratamiento de daño articular
ITUA20161797A1 (it) Iniettore di gas diretto con fughe ridotte
ES1228444Y (es) Sistema para automatizar el encendido, ajuste y/o apagado de uno o más accesorios para placas de cocción
GT201400151A (es) Cucurbitáceas androicas, procedimientos de obtención y utilizaciones de estas cucurbitáceas
CL2018000275A1 (es) Metodo para el tratamiento de las enfermedades de plantas
CL2016000014A1 (es) Un elemento de distribución de tensión que alivia los esfuerzos en las uniones o costuras de soldadura para una unión de un casco de molino que comprende un cuerpo alargado que tiene un extremo proximal; sistema de distribucion de tension; montaje de distribución de tensión; seccion de casco de molino y casco de molino.
DK3429577T3 (da) N-lipoinaminosyre eller peptid, derivater og anvendelser deraf
DK3219876T3 (da) Strømmast med flangeforbundne rørsegmenter
SG11201706183QA (en) System and method for controlling fuel supply of ship engine
ES2772573R2 (es) Sistema helice tobera aceleradora para impulsar barcos
DK3410013T3 (da) Forbrændingsgastilførselssystem
DK3310609T3 (da) Energiforsynings- og drivkraftsystem til skib og offshore enheder
NO345090B1 (en) Peptides for the inhibition of trypsin and sea lice infestation
IT201900022389A1 (it) Sistema di alimentazione.
IT201700105633A1 (it) Sistema di alimentazione e comando a bassissima tensione in particolare per lampade a led.
UA34534S (uk) Труба підводу палива