CY1117936T1 - Βελτιωμενες μεθοδοι για τον καθαρισμο ανασυνδυασμενων φορεων aav - Google Patents
Βελτιωμενες μεθοδοι για τον καθαρισμο ανασυνδυασμενων φορεων aavInfo
- Publication number
- CY1117936T1 CY1117936T1 CY20161100843T CY161100843T CY1117936T1 CY 1117936 T1 CY1117936 T1 CY 1117936T1 CY 20161100843 T CY20161100843 T CY 20161100843T CY 161100843 T CY161100843 T CY 161100843T CY 1117936 T1 CY1117936 T1 CY 1117936T1
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- CY
- Cyprus
- Prior art keywords
- cleaning
- improved methods
- aav
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- bodies
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
- C12N7/02—Recovery or purification
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- C12N15/861—Adenoviral vectors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Medicinal Preparation (AREA)
Abstract
Στο παρόν παρέχονται μέθοδοι για τον καθαρισμό ανασυνδυασμένων αδενο-σχετιζόμενων ιικών φορέων (rAAV) που μπορεί να χρησιμοποιηθούν για γονιδιακή μεταφορά και συγκεκριμένα για γονιδιακή θεραπεία ή εμβολιασμό. Ανασυνδυασμένοι AAV φορείς της εφεύρεσης είναι ουσιαστικά ελεύθεροι από προσμίξεις κατά τη διεργασία, συμπεριλαμβανομένων συστατικών παραγωγής, όπως κυτταρικών νουκλεϊκών οξέων, κυτταρικών πρωτεϊνών, βοηθητικού ιού, και συστατικών του μέσου.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US18760109P | 2009-06-16 | 2009-06-16 | |
PCT/US2010/038897 WO2010148143A1 (en) | 2009-06-16 | 2010-06-16 | Improved methods for purification of recombinant aav vectors |
Publications (1)
Publication Number | Publication Date |
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CY1117936T1 true CY1117936T1 (el) | 2017-05-17 |
Family
ID=43356746
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20161100843T CY1117936T1 (el) | 2009-06-16 | 2016-08-29 | Βελτιωμενες μεθοδοι για τον καθαρισμο ανασυνδυασμενων φορεων aav |
CY20181101103T CY1121442T1 (el) | 2009-06-16 | 2018-10-25 | Βελτιωμενες μεθοδοι για τον καθαρισμο ανασυνδυασμενων φορεων aav |
CY20211100639T CY1124323T1 (el) | 2009-06-16 | 2021-07-14 | Βελτιωμενες μεθοδοι για τον καθαρισμο ανασυνδυασμενων φορεων aav |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20181101103T CY1121442T1 (el) | 2009-06-16 | 2018-10-25 | Βελτιωμενες μεθοδοι για τον καθαρισμο ανασυνδυασμενων φορεων aav |
CY20211100639T CY1124323T1 (el) | 2009-06-16 | 2021-07-14 | Βελτιωμενες μεθοδοι για τον καθαρισμο ανασυνδυασμενων φορεων aav |
Country Status (24)
Country | Link |
---|---|
US (3) | US10017746B2 (el) |
EP (4) | EP3444335B1 (el) |
JP (3) | JP5956331B2 (el) |
KR (2) | KR101812813B1 (el) |
CN (2) | CN105861454B (el) |
BR (1) | BRPI1015176B1 (el) |
CA (2) | CA2764176C (el) |
CY (3) | CY1117936T1 (el) |
DK (2) | DK3067417T3 (el) |
ES (3) | ES2588990T3 (el) |
HK (1) | HK1165830A1 (el) |
HR (2) | HRP20181743T1 (el) |
HU (3) | HUE054940T2 (el) |
IL (3) | IL216960A (el) |
LT (2) | LT3444335T (el) |
MX (2) | MX352986B (el) |
PL (2) | PL3067417T3 (el) |
PT (3) | PT2443233T (el) |
RS (2) | RS62086B1 (el) |
RU (1) | RU2016119739A (el) |
SG (2) | SG176283A1 (el) |
SI (2) | SI3067417T1 (el) |
TR (1) | TR201815937T4 (el) |
WO (1) | WO2010148143A1 (el) |
Families Citing this family (96)
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