CY1115543T1 - Dνα ανασυνδυασμος ειδικης ακολουθιας σε ευκαρυωτικα κυτταρα - Google Patents

Dνα ανασυνδυασμος ειδικης ακολουθιας σε ευκαρυωτικα κυτταρα

Info

Publication number
CY1115543T1
CY1115543T1 CY20141100747T CY141100747T CY1115543T1 CY 1115543 T1 CY1115543 T1 CY 1115543T1 CY 20141100747 T CY20141100747 T CY 20141100747T CY 141100747 T CY141100747 T CY 141100747T CY 1115543 T1 CY1115543 T1 CY 1115543T1
Authority
CY
Cyprus
Prior art keywords
dna
specific sequences
sequence
dna recombinant
recombinant
Prior art date
Application number
CY20141100747T
Other languages
English (en)
Inventor
Peter Dröge
Barbara Enenkel
Original Assignee
Boehringer Ingelheim Pharma Gmbh & Co. Kg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA2413175A external-priority patent/CA2413175C/en
Priority claimed from US10/310,695 external-priority patent/US7491539B2/en
Application filed by Boehringer Ingelheim Pharma Gmbh & Co. Kg filed Critical Boehringer Ingelheim Pharma Gmbh & Co. Kg
Publication of CY1115543T1 publication Critical patent/CY1115543T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Η παρούσα εφεύρεση αναφέρεται σε μία μέθοδο ανασυνδυασμού DΝΑ ειδικής ακολουθίας σε ευκαρυωτικά κύτταρα, που περιλαμβάνει την εισαγωγή ενός πρώτου DΝΑ που περιλαμβάνει μία νουκλεοτιδική ακολουθία που περιέχει τουλάχιστον μία ακολουθία ανασυνδυασμού σε ένα κύτταρο, εισαγωγή ενός δεύτερου DΝΑ που περιλαμβάνει μία νουκλεοτιδική ακολουθία που περιέχει τουλάχιστον μία περαιτέρω ακολουθία ανασυνδυασμού σε ένα κύτταρο, και διεξαγωγή του ανασυνδυασμού ειδικής ακολουθίας με μία ιντεγράση Ιnt ενός βακτηριοφάγου lambda.
CY20141100747T 2002-11-28 2014-09-16 Dνα ανασυνδυασμος ειδικης ακολουθιας σε ευκαρυωτικα κυτταρα CY1115543T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CA2413175A CA2413175C (en) 2002-11-28 2002-11-28 Sequence specific dna recombination in eukaryotic cells
US10/310,695 US7491539B2 (en) 2002-12-05 2002-12-05 Sequence specific DNA recombination in eukaryotic cells
EP03785683.8A EP1565562B1 (en) 2002-11-28 2003-11-28 Sequence specific dna recombination in eukaryotic cells

Publications (1)

Publication Number Publication Date
CY1115543T1 true CY1115543T1 (el) 2017-01-04

Family

ID=32394628

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20141100747T CY1115543T1 (el) 2002-11-28 2014-09-16 Dνα ανασυνδυασμος ειδικης ακολουθιας σε ευκαρυωτικα κυτταρα

Country Status (19)

Country Link
US (1) US7968341B2 (el)
EP (1) EP1565562B1 (el)
JP (1) JP2006507847A (el)
KR (1) KR101099380B1 (el)
CN (1) CN1717489B (el)
AR (1) AR042146A1 (el)
AU (1) AU2003294743B2 (el)
BR (1) BR0316822A (el)
CY (1) CY1115543T1 (el)
DK (1) DK1565562T3 (el)
ES (1) ES2507665T3 (el)
HK (1) HK1081998A1 (el)
IL (1) IL168101A (el)
MX (1) MXPA05005080A (el)
NZ (1) NZ540549A (el)
PT (1) PT1565562E (el)
SI (1) SI1565562T1 (el)
TW (1) TW200502390A (el)
WO (1) WO2004048584A1 (el)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200502390A (en) 2002-11-28 2005-01-16 Peter Droege Sequence specific DNA recombination in eukaryotic cells
WO2004101800A1 (en) * 2003-05-15 2004-11-25 Medigene Ag Dna constructs and vectors for the inducible expression of nucleotide sequences
WO2009068645A1 (en) * 2007-11-30 2009-06-04 Boehringer Ingelheim Pharma Gmbh & Co. Kg Novel recombination sequences
SG170652A1 (en) * 2009-11-06 2011-05-30 Agency Science Tech & Res Muteins of the bacteriophage lambda integrases
CN102234656A (zh) * 2010-04-29 2011-11-09 中国科学院上海生命科学研究院 在哺乳动物细胞内进行位点特异性重组的方法及系统
CN106906238B (zh) * 2015-12-22 2020-08-14 中国科学院分子植物科学卓越创新中心 一种链霉菌抗生素生物合成基因簇多拷贝扩增方法及应用
CN111019873B (zh) * 2018-10-09 2022-08-19 安徽吐露港生物科技有限公司 在食气梭菌基因组上快速整合大片段dna的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5861273A (en) 1993-12-21 1999-01-19 Celtrix Phamraceuticals, Inc. Chromosomal expression of heterologous genes in bacterial cells
US5928914A (en) 1996-06-14 1999-07-27 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Methods and compositions for transforming cells
US20030148463A1 (en) 1997-04-14 2003-08-07 Micromet Ag Novel method for the production of anti-human antigen receptors and uses thereof
DE19941186A1 (de) 1999-08-30 2001-03-01 Peter Droege Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen
EP1250453B1 (en) 1999-12-10 2008-04-09 Invitrogen Corporation Use of multiple recombination sites with unique specificity in recombinational cloning
US20060130179A1 (en) 2002-03-29 2006-06-15 Suttie Janet L Lambda integrase mediated recombination in plants
US7491539B2 (en) 2002-12-05 2009-02-17 Boehringer Ingelheim Pharma Kg Sequence specific DNA recombination in eukaryotic cells
TW200502390A (en) 2002-11-28 2005-01-16 Peter Droege Sequence specific DNA recombination in eukaryotic cells

Also Published As

Publication number Publication date
US7968341B2 (en) 2011-06-28
HK1081998A1 (en) 2006-05-26
AR042146A1 (es) 2005-06-08
PT1565562E (pt) 2014-09-24
IL168101A (en) 2010-12-30
TW200502390A (en) 2005-01-16
CN1717489A (zh) 2006-01-04
NZ540549A (en) 2006-11-30
BR0316822A (pt) 2005-10-18
JP2006507847A (ja) 2006-03-09
KR20050090388A (ko) 2005-09-13
KR101099380B1 (ko) 2011-12-29
AU2003294743A1 (en) 2004-06-18
WO2004048584A1 (en) 2004-06-10
DK1565562T3 (da) 2014-10-20
US20070148773A1 (en) 2007-06-28
EP1565562A1 (en) 2005-08-24
SI1565562T1 (sl) 2014-11-28
MXPA05005080A (es) 2005-10-19
EP1565562B1 (en) 2014-07-16
CN1717489B (zh) 2012-03-21
ES2507665T3 (es) 2014-10-15
AU2003294743B2 (en) 2009-10-08

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