BR0316822A - Recombinação de dna especìfica da sequência em células eucarióticas - Google Patents

Recombinação de dna especìfica da sequência em células eucarióticas

Info

Publication number
BR0316822A
BR0316822A BR0316822-0A BR0316822A BR0316822A BR 0316822 A BR0316822 A BR 0316822A BR 0316822 A BR0316822 A BR 0316822A BR 0316822 A BR0316822 A BR 0316822A
Authority
BR
Brazil
Prior art keywords
sequence
recombination
dna
eukaryotic cells
specific dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
BR0316822-0A
Other languages
English (en)
Inventor
Peter Droege
Barbara Enenkel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim Pharma GmbH Co KG
Original Assignee
Boehringer Ingelheim Pharma GmbH Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA2413175A external-priority patent/CA2413175C/en
Priority claimed from US10/310,695 external-priority patent/US7491539B2/en
Application filed by Boehringer Ingelheim Pharma GmbH Co KG filed Critical Boehringer Ingelheim Pharma GmbH Co KG
Publication of BR0316822A publication Critical patent/BR0316822A/pt
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"RECOMBINAçãO DE DNA ESPECìFICA DA SEQuêNCIA EM CéLULAS EUCARIóTICAS". A presente invenção diz respeito a um método de recombinação específica da seq³ência de DNA em células eucarióticas, que compreende a introdução de um primeiro DNA que compreende uma seq³ência de nucleotídeos contendo, pelo menos, uma seq³ência de recombinação em uma célula, a introdução de um segundo DNA que compreende uma seq³ência de nucleotídeos contendo, pelo menos, uma seq³ência adicional de recombinação em uma célula, e a realização da recombinação específica da seq³ência por meio de um bacteriófago lambda integrase Int.
BR0316822-0A 2002-11-28 2003-11-28 Recombinação de dna especìfica da sequência em células eucarióticas Pending BR0316822A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CA2413175A CA2413175C (en) 2002-11-28 2002-11-28 Sequence specific dna recombination in eukaryotic cells
US10/310,695 US7491539B2 (en) 2002-12-05 2002-12-05 Sequence specific DNA recombination in eukaryotic cells
PCT/EP2003/013414 WO2004048584A1 (en) 2002-11-28 2003-11-28 Sequence specific dna recombination in eukaryotic cells

Publications (1)

Publication Number Publication Date
BR0316822A true BR0316822A (pt) 2005-10-18

Family

ID=32394628

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0316822-0A Pending BR0316822A (pt) 2002-11-28 2003-11-28 Recombinação de dna especìfica da sequência em células eucarióticas

Country Status (19)

Country Link
US (1) US7968341B2 (pt)
EP (1) EP1565562B1 (pt)
JP (1) JP2006507847A (pt)
KR (1) KR101099380B1 (pt)
CN (1) CN1717489B (pt)
AR (1) AR042146A1 (pt)
AU (1) AU2003294743B2 (pt)
BR (1) BR0316822A (pt)
CY (1) CY1115543T1 (pt)
DK (1) DK1565562T3 (pt)
ES (1) ES2507665T3 (pt)
HK (1) HK1081998A1 (pt)
IL (1) IL168101A (pt)
MX (1) MXPA05005080A (pt)
NZ (1) NZ540549A (pt)
PT (1) PT1565562E (pt)
SI (1) SI1565562T1 (pt)
TW (1) TW200502390A (pt)
WO (1) WO2004048584A1 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200502390A (en) 2002-11-28 2005-01-16 Peter Droege Sequence specific DNA recombination in eukaryotic cells
WO2004101800A1 (en) * 2003-05-15 2004-11-25 Medigene Ag Dna constructs and vectors for the inducible expression of nucleotide sequences
CA2706050C (en) 2007-11-30 2019-01-22 Boehringer Ingelheim Pharma Gmbh & Co. Kg Novel att recombination sequences
SG170652A1 (en) * 2009-11-06 2011-05-30 Agency Science Tech & Res Muteins of the bacteriophage lambda integrases
CN102234656A (zh) * 2010-04-29 2011-11-09 中国科学院上海生命科学研究院 在哺乳动物细胞内进行位点特异性重组的方法及系统
CN106906238B (zh) * 2015-12-22 2020-08-14 中国科学院分子植物科学卓越创新中心 一种链霉菌抗生素生物合成基因簇多拷贝扩增方法及应用
CN111019873B (zh) * 2018-10-09 2022-08-19 安徽吐露港生物科技有限公司 在食气梭菌基因组上快速整合大片段dna的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5861273A (en) 1993-12-21 1999-01-19 Celtrix Phamraceuticals, Inc. Chromosomal expression of heterologous genes in bacterial cells
US5928914A (en) 1996-06-14 1999-07-27 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Methods and compositions for transforming cells
US20030148463A1 (en) 1997-04-14 2003-08-07 Micromet Ag Novel method for the production of anti-human antigen receptors and uses thereof
DE19941186A1 (de) 1999-08-30 2001-03-01 Peter Droege Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen
WO2001042509A1 (en) * 1999-12-10 2001-06-14 Invitrogen Corporation Use of multiple recombination sites with unique specificity in recombinational cloning
CA2480642C (en) 2002-03-29 2012-10-16 Janet Louise Suttie Lambda integrase mediated recombination in plants
TW200502390A (en) 2002-11-28 2005-01-16 Peter Droege Sequence specific DNA recombination in eukaryotic cells
US7491539B2 (en) 2002-12-05 2009-02-17 Boehringer Ingelheim Pharma Kg Sequence specific DNA recombination in eukaryotic cells

Also Published As

Publication number Publication date
EP1565562A1 (en) 2005-08-24
PT1565562E (pt) 2014-09-24
CY1115543T1 (el) 2017-01-04
WO2004048584A1 (en) 2004-06-10
CN1717489B (zh) 2012-03-21
KR20050090388A (ko) 2005-09-13
MXPA05005080A (es) 2005-10-19
IL168101A (en) 2010-12-30
KR101099380B1 (ko) 2011-12-29
ES2507665T3 (es) 2014-10-15
JP2006507847A (ja) 2006-03-09
AU2003294743B2 (en) 2009-10-08
DK1565562T3 (da) 2014-10-20
CN1717489A (zh) 2006-01-04
US20070148773A1 (en) 2007-06-28
TW200502390A (en) 2005-01-16
NZ540549A (en) 2006-11-30
AU2003294743A1 (en) 2004-06-18
EP1565562B1 (en) 2014-07-16
AR042146A1 (es) 2005-06-08
US7968341B2 (en) 2011-06-28
SI1565562T1 (sl) 2014-11-28
HK1081998A1 (en) 2006-05-26

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 13A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]