AR042146A1 - Recombinacion de adn especifica de secuencias en celulas eucariotas - Google Patents
Recombinacion de adn especifica de secuencias en celulas eucariotasInfo
- Publication number
- AR042146A1 AR042146A1 ARP030104396A ARP030104396A AR042146A1 AR 042146 A1 AR042146 A1 AR 042146A1 AR P030104396 A ARP030104396 A AR P030104396A AR P030104396 A ARP030104396 A AR P030104396A AR 042146 A1 AR042146 A1 AR 042146A1
- Authority
- AR
- Argentina
- Prior art keywords
- sequence
- derivative
- dna
- cell
- attp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Método de recombinación de ADN específica de secuencias en células eucariotas, que comprende la introducción en una célula de un primer ADN que comprende una secuencia de nucléotidos que contiene al menos la secuencia recombinante, la introducción en una célula de un segundo ADN que comprende una secuencia de nucléotidos que contiene al menos una secuencia recombinante adicional y llevar a cabo una recombinación específica de secuencias con una integrasa Int del bacteriófago lambda. Reivindicación 1: Un método de recombinación de ADN específica de secuencias en una célula eucariota, caracterizado porque comprende a) introducir un ADN que comprende una primera secuencia attB, attP, attL o attR, o un derivado de la misma, en una célula; b) introducir un ADN que comprende una segunda secuencia attB, attP, attL o attR, o un derivado de la misma, en una célula, donde si dicha primera secuencia de ADN comprende una secuencia attB, o un derivado de la misma, dicha segunda secuencia comprende una secuencia attB, attL o attR, o un derivado de la misma, o donde si dicha primera secuencia de ADN comprende una secuencia attP, o un derivado de la misma, dicha segunda secuencia comprende una secuencia attP, attL o attR, o un derivado de la misma, o donde si dicha primera secuencia de ADN comprende una secuencia attL, o un derivado de la misma, dicha segunda secuencia comprende una secuencia attB, attP o attL, o un derivado de la misma, o donde si dicha primera secuencia de ADN comprende una secuencia attR, o un derivado de la misma, dicha segunda secuencia comprende una secuencia attB, attP o attR, o un derivado de la misma; y c) llevar a cabo una recombinación específica de secuencia con la integrasa Int. Del bacteriófago lambda. Reivindicación 20: El método acorde con cualquiera de las reivindicaciones precedentes, caracterizado porque dicho polipéptido de interés es un anticuerpo, una hormona o un factor de crecimiento. Reivindicación 23: El método acorde con la reivindicación 22, caracterizado porque la célula de hámster es una célula BHK o CHO y la célula de ratón es una célula de mieloma murina, preferentemente una célula NS0 y Sp2/0.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA2413175A CA2413175C (en) | 2002-11-28 | 2002-11-28 | Sequence specific dna recombination in eukaryotic cells |
| US10/310,695 US7491539B2 (en) | 2002-12-05 | 2002-12-05 | Sequence specific DNA recombination in eukaryotic cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR042146A1 true AR042146A1 (es) | 2005-06-08 |
Family
ID=32394628
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP030104396A Pending AR042146A1 (es) | 2002-11-28 | 2003-11-28 | Recombinacion de adn especifica de secuencias en celulas eucariotas |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US7968341B2 (es) |
| EP (1) | EP1565562B1 (es) |
| JP (1) | JP2006507847A (es) |
| KR (1) | KR101099380B1 (es) |
| CN (1) | CN1717489B (es) |
| AR (1) | AR042146A1 (es) |
| AU (1) | AU2003294743B2 (es) |
| BR (1) | BR0316822A (es) |
| CY (1) | CY1115543T1 (es) |
| DK (1) | DK1565562T3 (es) |
| ES (1) | ES2507665T3 (es) |
| HK (1) | HK1081998A1 (es) |
| IL (1) | IL168101A (es) |
| MX (1) | MXPA05005080A (es) |
| NZ (1) | NZ540549A (es) |
| PT (1) | PT1565562E (es) |
| SI (1) | SI1565562T1 (es) |
| TW (1) | TW200502390A (es) |
| WO (1) | WO2004048584A1 (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW200502390A (en) | 2002-11-28 | 2005-01-16 | Peter Droege | Sequence specific DNA recombination in eukaryotic cells |
| WO2004101800A1 (en) * | 2003-05-15 | 2004-11-25 | Medigene Ag | Dna constructs and vectors for the inducible expression of nucleotide sequences |
| US8404486B2 (en) | 2007-11-30 | 2013-03-26 | Boehringer Ingelheim International Gmbh | Recombination sequences |
| SG170652A1 (en) * | 2009-11-06 | 2011-05-30 | Agency Science Tech & Res | Muteins of the bacteriophage lambda integrases |
| CN102234656A (zh) * | 2010-04-29 | 2011-11-09 | 中国科学院上海生命科学研究院 | 在哺乳动物细胞内进行位点特异性重组的方法及系统 |
| CN106906238B (zh) * | 2015-12-22 | 2020-08-14 | 中国科学院分子植物科学卓越创新中心 | 一种链霉菌抗生素生物合成基因簇多拷贝扩增方法及应用 |
| CN111019873B (zh) * | 2018-10-09 | 2022-08-19 | 安徽吐露港生物科技有限公司 | 在食气梭菌基因组上快速整合大片段dna的方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5861273A (en) | 1993-12-21 | 1999-01-19 | Celtrix Phamraceuticals, Inc. | Chromosomal expression of heterologous genes in bacterial cells |
| US5928914A (en) | 1996-06-14 | 1999-07-27 | Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University | Methods and compositions for transforming cells |
| US20030148463A1 (en) | 1997-04-14 | 2003-08-07 | Micromet Ag | Novel method for the production of anti-human antigen receptors and uses thereof |
| DE19941186A1 (de) * | 1999-08-30 | 2001-03-01 | Peter Droege | Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen |
| AU785483B2 (en) * | 1999-12-10 | 2007-09-20 | Invitrogen Corporation | Use of multiple recombination sites with unique specificity in recombinational cloning |
| US7351877B2 (en) | 2002-03-29 | 2008-04-01 | Syngenta Participations Ag | Lambda integrase mediated recombination in plants |
| TW200502390A (en) | 2002-11-28 | 2005-01-16 | Peter Droege | Sequence specific DNA recombination in eukaryotic cells |
| US7491539B2 (en) | 2002-12-05 | 2009-02-17 | Boehringer Ingelheim Pharma Kg | Sequence specific DNA recombination in eukaryotic cells |
-
2003
- 2003-11-27 TW TW092133348A patent/TW200502390A/zh unknown
- 2003-11-28 KR KR1020057009709A patent/KR101099380B1/ko active IP Right Grant
- 2003-11-28 AU AU2003294743A patent/AU2003294743B2/en not_active Expired
- 2003-11-28 MX MXPA05005080A patent/MXPA05005080A/es active IP Right Grant
- 2003-11-28 ES ES03785683.8T patent/ES2507665T3/es not_active Expired - Lifetime
- 2003-11-28 US US10/536,933 patent/US7968341B2/en active Active
- 2003-11-28 PT PT37856838T patent/PT1565562E/pt unknown
- 2003-11-28 EP EP03785683.8A patent/EP1565562B1/en not_active Expired - Lifetime
- 2003-11-28 JP JP2005510242A patent/JP2006507847A/ja not_active Withdrawn
- 2003-11-28 NZ NZ540549A patent/NZ540549A/en not_active IP Right Cessation
- 2003-11-28 WO PCT/EP2003/013414 patent/WO2004048584A1/en active Application Filing
- 2003-11-28 DK DK03785683.8T patent/DK1565562T3/da active
- 2003-11-28 CN CN2003801045692A patent/CN1717489B/zh not_active Expired - Lifetime
- 2003-11-28 AR ARP030104396A patent/AR042146A1/es active Pending
- 2003-11-28 BR BR0316822-0A patent/BR0316822A/pt active Pending
- 2003-11-28 SI SI200332387T patent/SI1565562T1/sl unknown
-
2005
- 2005-04-18 IL IL168101A patent/IL168101A/en not_active IP Right Cessation
-
2006
- 2006-02-24 HK HK06102468.3A patent/HK1081998A1/xx not_active IP Right Cessation
-
2014
- 2014-09-16 CY CY20141100747T patent/CY1115543T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003294743A1 (en) | 2004-06-18 |
| EP1565562B1 (en) | 2014-07-16 |
| ES2507665T3 (es) | 2014-10-15 |
| US7968341B2 (en) | 2011-06-28 |
| CN1717489A (zh) | 2006-01-04 |
| PT1565562E (pt) | 2014-09-24 |
| WO2004048584A1 (en) | 2004-06-10 |
| TW200502390A (en) | 2005-01-16 |
| IL168101A (en) | 2010-12-30 |
| BR0316822A (pt) | 2005-10-18 |
| JP2006507847A (ja) | 2006-03-09 |
| NZ540549A (en) | 2006-11-30 |
| CN1717489B (zh) | 2012-03-21 |
| SI1565562T1 (sl) | 2014-11-28 |
| AU2003294743B2 (en) | 2009-10-08 |
| US20070148773A1 (en) | 2007-06-28 |
| EP1565562A1 (en) | 2005-08-24 |
| MXPA05005080A (es) | 2005-10-19 |
| KR101099380B1 (ko) | 2011-12-29 |
| KR20050090388A (ko) | 2005-09-13 |
| CY1115543T1 (el) | 2017-01-04 |
| HK1081998A1 (en) | 2006-05-26 |
| DK1565562T3 (da) | 2014-10-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yang et al. | Torsional control of double‐stranded DNA branch migration | |
| DE69833457D1 (de) | Neuartige methode zur integration von fremd-dna ins eukaryotische genom | |
| ATE267866T1 (de) | Kurze externe führungssequenzen | |
| MXPA03002572A (es) | Secuencias de nucleotidos que intervienen en la fertilidad de los machos y metodo de su uso. | |
| NO20003058L (no) | Massespektrometriske metoder for sekvensering av nukleinsyrer | |
| ATE356824T1 (de) | L-ribo-lna analoge | |
| DK0973928T3 (da) | DNA-baseret transposonsystem til indføring af nukleinsyre i DNA i en celle | |
| ATE296356T1 (de) | Methode zur spezifischen integration von genen in säugetierzellen durch homologe rekombination, und vektoren zu deren durchführung | |
| ATE294229T1 (de) | Verfahren zur trocknung von kompetenten zellen | |
| CN112979821B (zh) | 一种提高基因编辑效率的融合蛋白及其应用 | |
| DE69932599D1 (de) | Künstliche chromosomen, die die genetische information für die bildung von rekombinantem virus enthalten | |
| CY1115543T1 (el) | Dνα ανασυνδυασμος ειδικης ακολουθιας σε ευκαρυωτικα κυτταρα | |
| MXPA06000092A (es) | Transformacion de cloroplasto de la lenteja de agua. | |
| MXPA03006984A (es) | Secuencia de nucleotidos que median la fertilidad masculina y metodo de uso de la misma. | |
| ID29579A (id) | Analisis genomik multi-loci dengan metode pengurutan siklus yang diperbaiki | |
| DK1504098T4 (da) | Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion | |
| JPH09508282A (ja) | 短いオリゴヌクレオチドの配列決定法 | |
| ES2382868T3 (es) | Cebadores para amplificación de ácido nucleico en la detección ARNm de gen constitutivo y método de ensayo que usa estos cebadores | |
| Nishigaki et al. | Y-ligation: an efficient method for ligating single-stranded DNAs and RNAs with T4 RNA ligase | |
| AR069511A1 (es) | Secuencias de recombinacion att que permiten la generacion de un sistema mejorado de expresion de proteinas | |
| Kikuno et al. | Rapid rate of rodent evolution | |
| AR016263A1 (es) | Un constructo de adn que altera la expresion de un gen g-csf endogeno en una celula de mamifero en la integracion al genoma de la celula a traves derecombinacion homologa, una celula recombinante homologamente establemente transfectada con el constructo de adn, un metodo de alteracion de la expresio | |
| Boán et al. | Inhibition of DNA synthesis by K+-stabilised G-quadruplex promotes allelic preferential amplification | |
| Williams et al. | Telomere formation on macronuclear chromosomes of Oxytricha trifallax and O. fallax: alternatively processed regions have multiple telomere addition sites | |
| WO2023085117A1 (ja) | 鋳型ｄｎａの製造方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant, registration |