SG11201908782XA - Method for converting nucleic acid sequence of cell specifically converting nucleic acid base of targeted dna using cell endogenous dna modifying enzyme, and molecular complex used therein - Google Patents
Method for converting nucleic acid sequence of cell specifically converting nucleic acid base of targeted dna using cell endogenous dna modifying enzyme, and molecular complex used thereinInfo
- Publication number
- SG11201908782XA SG11201908782XA SG11201908782XA SG11201908782XA SG 11201908782X A SG11201908782X A SG 11201908782XA SG 11201908782X A SG11201908782X A SG 11201908782XA SG 11201908782X A SG11201908782X A SG 11201908782XA
- Authority
- SG
- Singapore
- Prior art keywords
- nucleic acid
- cell
- dna
- modifying enzyme
- converting nucleic
- Prior art date
Links
- 108020004414 DNA Proteins 0.000 title abstract 9
- 150000007523 nucleic acids Chemical group 0.000 title abstract 5
- 102000004190 Enzymes Human genes 0.000 title abstract 4
- 108090000790 Enzymes Proteins 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 title abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 5
- 125000003729 nucleotide group Chemical group 0.000 abstract 5
- 230000001939 inductive effect Effects 0.000 abstract 1
- 230000004936 stimulating effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- C12N2330/00—Production
- C12N2330/50—Biochemical production, i.e. in a transformed host cell
- C12N2330/51—Specially adapted vectors
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- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/106—Plasmid DNA for vertebrates
- C12N2800/107—Plasmid DNA for vertebrates for mammalian
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
METHOD FOR CONVERTING NUCLEIC ACID SEQUENCE OF CELL SPECIFICALLY CONVERTING NUCLEIC ACID BASE OF TARGETED DNA USING CELL ENDOGENOUS DNA MODIFYING ENZYME, AND MOLECULAR COMPLEX USED THEREIN Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a io factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more 15 nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site. [No suitable figure] 58
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2017056727 | 2017-03-22 | ||
PCT/JP2018/011198 WO2018174097A1 (en) | 2017-03-22 | 2018-03-20 | Method for converting nucleic acid sequence of cell specifically converting nucleic acid base of targeted dna using cell endogenous dna modifying enzyme, and molecular complex used therein |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11201908782XA true SG11201908782XA (en) | 2019-10-30 |
Family
ID=63585387
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201908782X SG11201908782XA (en) | 2017-03-22 | 2018-03-20 | Method for converting nucleic acid sequence of cell specifically converting nucleic acid base of targeted dna using cell endogenous dna modifying enzyme, and molecular complex used therein |
Country Status (9)
Country | Link |
---|---|
US (2) | US11845953B2 (en) |
EP (1) | EP3604519A4 (en) |
JP (1) | JP7133856B2 (en) |
KR (1) | KR102280546B1 (en) |
CN (2) | CN110446782B (en) |
BR (1) | BR112019019673A2 (en) |
CA (1) | CA3057432C (en) |
SG (1) | SG11201908782XA (en) |
WO (1) | WO2018174097A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019222545A1 (en) | 2018-05-16 | 2019-11-21 | Synthego Corporation | Methods and systems for guide rna design and use |
EP4118199A4 (en) * | 2020-03-11 | 2024-03-06 | North Carolina State University | Compositions, methods, and systems for genome editing technology |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2003251286B2 (en) | 2002-01-23 | 2007-08-16 | The University Of Utah Research Foundation | Targeted chromosomal mutagenesis using zinc finger nucleases |
US7393923B2 (en) * | 2004-11-08 | 2008-07-01 | The Regents Of The University Of California | Red-shifted fluorescent proteins mPlum and mRaspberry and polynucleotides encoding the same |
US8198046B2 (en) * | 2006-07-11 | 2012-06-12 | Danisco Us Inc. | KEX2 cleavage regions of recombinant fusion proteins |
AU2008223544B2 (en) | 2007-03-02 | 2014-06-05 | Dupont Nutrition Biosciences Aps | Cultures with improved phage resistance |
US20110104787A1 (en) * | 2009-11-05 | 2011-05-05 | President And Fellows Of Harvard College | Fusion Peptides That Bind to and Modify Target Nucleic Acid Sequences |
CN106834320B (en) | 2009-12-10 | 2021-05-25 | 明尼苏达大学董事会 | TAL effector-mediated DNA modification |
JP2013128413A (en) | 2010-03-11 | 2013-07-04 | Kyushu Univ | Method for modifying rna-binding protein using ppr motif |
JP2011231053A (en) | 2010-04-28 | 2011-11-17 | Kumamoto Univ | Apobec3 expression improver and anti-hiv agent |
EP3115457B1 (en) | 2014-03-05 | 2019-10-02 | National University Corporation Kobe University | Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same |
EP4434997A2 (en) * | 2014-10-30 | 2024-09-25 | President And Fellows Of Harvard College | Delivery of negatively charged proteins using cationic lipids |
JP6153180B2 (en) | 2014-11-04 | 2017-06-28 | 国立大学法人神戸大学 | Method for modifying genomic sequence, which specifically introduces mutation into DNA sequence targeted by abasic reaction, and molecular complex used therefor |
WO2016164889A1 (en) | 2015-04-09 | 2016-10-13 | Health Research, Inc. | Use of atpenin to activate innate immunity |
EP3322804B1 (en) | 2015-07-15 | 2021-09-01 | Rutgers, The State University of New Jersey | Nuclease-independent targeted gene editing platform and uses thereof |
WO2017015015A1 (en) * | 2015-07-17 | 2017-01-26 | Emory University | Crispr-associated protein from francisella and uses related thereto |
JP2017056726A (en) | 2015-09-16 | 2017-03-23 | 日本製紙株式会社 | Thermosensitive recording medium |
KR20180110144A (en) * | 2016-02-26 | 2018-10-08 | 란자테크 뉴질랜드 리미티드 | CRISPR / CAS system for C1-immobilized bacteria |
US20190309288A1 (en) * | 2016-08-18 | 2019-10-10 | The Board Of Trustees Of The Leland Stanford Junior University | Targeted mutagenesis |
WO2019103020A1 (en) * | 2017-11-22 | 2019-05-31 | 国立大学法人神戸大学 | Complex for genome editing having stability and few side-effects, and nucleic acid coding same |
-
2018
- 2018-03-20 SG SG11201908782X patent/SG11201908782XA/en unknown
- 2018-03-20 US US16/496,311 patent/US11845953B2/en active Active
- 2018-03-20 EP EP18770389.7A patent/EP3604519A4/en active Pending
- 2018-03-20 CA CA3057432A patent/CA3057432C/en active Active
- 2018-03-20 BR BR112019019673A patent/BR112019019673A2/en active Search and Examination
- 2018-03-20 CN CN201880020115.3A patent/CN110446782B/en active Active
- 2018-03-20 JP JP2019507712A patent/JP7133856B2/en active Active
- 2018-03-20 KR KR1020197031042A patent/KR102280546B1/en active IP Right Grant
- 2018-03-20 WO PCT/JP2018/011198 patent/WO2018174097A1/en unknown
- 2018-03-20 CN CN202410165335.2A patent/CN118006597A/en active Pending
-
2023
- 2023-12-14 US US18/539,833 patent/US20240117384A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CA3057432A1 (en) | 2019-11-28 |
WO2018174097A1 (en) | 2018-09-27 |
CN118006597A (en) | 2024-05-10 |
US20240117384A1 (en) | 2024-04-11 |
EP3604519A1 (en) | 2020-02-05 |
CN110446782B (en) | 2024-03-01 |
KR102280546B1 (en) | 2021-07-22 |
CA3057432C (en) | 2023-08-08 |
US11845953B2 (en) | 2023-12-19 |
JPWO2018174097A1 (en) | 2020-01-30 |
CN110446782A (en) | 2019-11-12 |
BR112019019673A2 (en) | 2020-04-22 |
KR20190131081A (en) | 2019-11-25 |
US20200010856A1 (en) | 2020-01-09 |
JP7133856B2 (en) | 2022-09-09 |
EP3604519A4 (en) | 2021-01-06 |
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