SG11201908782XA - Method for converting nucleic acid sequence of cell specifically converting nucleic acid base of targeted dna using cell endogenous dna modifying enzyme, and molecular complex used therein - Google Patents

Method for converting nucleic acid sequence of cell specifically converting nucleic acid base of targeted dna using cell endogenous dna modifying enzyme, and molecular complex used therein

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Publication number
SG11201908782XA
SG11201908782XA SG11201908782XA SG11201908782XA SG 11201908782X A SG11201908782X A SG 11201908782XA SG 11201908782X A SG11201908782X A SG 11201908782XA SG 11201908782X A SG11201908782X A SG 11201908782XA
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Singapore
Prior art keywords
nucleic acid
cell
dna
modifying enzyme
converting nucleic
Prior art date
Application number
Inventor
Keiji Nishida
Akihiko Kondo
Takayuki Arazoe
Shin Yoshioka
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Univ Kobe Nat Univ Corp
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Application filed by Univ Kobe Nat Univ Corp filed Critical Univ Kobe Nat Univ Corp
Publication of SG11201908782XA publication Critical patent/SG11201908782XA/en

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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

METHOD FOR CONVERTING NUCLEIC ACID SEQUENCE OF CELL SPECIFICALLY CONVERTING NUCLEIC ACID BASE OF TARGETED DNA USING CELL ENDOGENOUS DNA MODIFYING ENZYME, AND MOLECULAR COMPLEX USED THEREIN Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a io factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more 15 nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site. [No suitable figure] 58
SG11201908782X 2017-03-22 2018-03-20 Method for converting nucleic acid sequence of cell specifically converting nucleic acid base of targeted dna using cell endogenous dna modifying enzyme, and molecular complex used therein SG11201908782XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2017056727 2017-03-22
PCT/JP2018/011198 WO2018174097A1 (en) 2017-03-22 2018-03-20 Method for converting nucleic acid sequence of cell specifically converting nucleic acid base of targeted dna using cell endogenous dna modifying enzyme, and molecular complex used therein

Publications (1)

Publication Number Publication Date
SG11201908782XA true SG11201908782XA (en) 2019-10-30

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US (2) US11845953B2 (en)
EP (1) EP3604519A4 (en)
JP (1) JP7133856B2 (en)
KR (1) KR102280546B1 (en)
CN (2) CN110446782B (en)
BR (1) BR112019019673A2 (en)
CA (1) CA3057432C (en)
SG (1) SG11201908782XA (en)
WO (1) WO2018174097A1 (en)

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WO2019222545A1 (en) 2018-05-16 2019-11-21 Synthego Corporation Methods and systems for guide rna design and use
EP4118199A4 (en) * 2020-03-11 2024-03-06 North Carolina State University Compositions, methods, and systems for genome editing technology

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AU2003251286B2 (en) 2002-01-23 2007-08-16 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
US7393923B2 (en) * 2004-11-08 2008-07-01 The Regents Of The University Of California Red-shifted fluorescent proteins mPlum and mRaspberry and polynucleotides encoding the same
US8198046B2 (en) * 2006-07-11 2012-06-12 Danisco Us Inc. KEX2 cleavage regions of recombinant fusion proteins
AU2008223544B2 (en) 2007-03-02 2014-06-05 Dupont Nutrition Biosciences Aps Cultures with improved phage resistance
US20110104787A1 (en) * 2009-11-05 2011-05-05 President And Fellows Of Harvard College Fusion Peptides That Bind to and Modify Target Nucleic Acid Sequences
CN106834320B (en) 2009-12-10 2021-05-25 明尼苏达大学董事会 TAL effector-mediated DNA modification
JP2013128413A (en) 2010-03-11 2013-07-04 Kyushu Univ Method for modifying rna-binding protein using ppr motif
JP2011231053A (en) 2010-04-28 2011-11-17 Kumamoto Univ Apobec3 expression improver and anti-hiv agent
EP3115457B1 (en) 2014-03-05 2019-10-02 National University Corporation Kobe University Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same
EP4434997A2 (en) * 2014-10-30 2024-09-25 President And Fellows Of Harvard College Delivery of negatively charged proteins using cationic lipids
JP6153180B2 (en) 2014-11-04 2017-06-28 国立大学法人神戸大学 Method for modifying genomic sequence, which specifically introduces mutation into DNA sequence targeted by abasic reaction, and molecular complex used therefor
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JP2017056726A (en) 2015-09-16 2017-03-23 日本製紙株式会社 Thermosensitive recording medium
KR20180110144A (en) * 2016-02-26 2018-10-08 란자테크 뉴질랜드 리미티드 CRISPR / CAS system for C1-immobilized bacteria
US20190309288A1 (en) * 2016-08-18 2019-10-10 The Board Of Trustees Of The Leland Stanford Junior University Targeted mutagenesis
WO2019103020A1 (en) * 2017-11-22 2019-05-31 国立大学法人神戸大学 Complex for genome editing having stability and few side-effects, and nucleic acid coding same

Also Published As

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CA3057432A1 (en) 2019-11-28
WO2018174097A1 (en) 2018-09-27
CN118006597A (en) 2024-05-10
US20240117384A1 (en) 2024-04-11
EP3604519A1 (en) 2020-02-05
CN110446782B (en) 2024-03-01
KR102280546B1 (en) 2021-07-22
CA3057432C (en) 2023-08-08
US11845953B2 (en) 2023-12-19
JPWO2018174097A1 (en) 2020-01-30
CN110446782A (en) 2019-11-12
BR112019019673A2 (en) 2020-04-22
KR20190131081A (en) 2019-11-25
US20200010856A1 (en) 2020-01-09
JP7133856B2 (en) 2022-09-09
EP3604519A4 (en) 2021-01-06

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