CY1114785T1 - Ανιχνευση και καταμετρηση μικροοργανισμων - Google Patents

Ανιχνευση και καταμετρηση μικροοργανισμων

Info

Publication number
CY1114785T1
CY1114785T1 CY20121100633T CY121100633T CY1114785T1 CY 1114785 T1 CY1114785 T1 CY 1114785T1 CY 20121100633 T CY20121100633 T CY 20121100633T CY 121100633 T CY121100633 T CY 121100633T CY 1114785 T1 CY1114785 T1 CY 1114785T1
Authority
CY
Cyprus
Prior art keywords
microorganisms
cell proliferation
proliferation inhibitor
sample
cell
Prior art date
Application number
CY20121100633T
Other languages
English (en)
Inventor
Yannick Fovet
Sam Dukan
Adrien Ducret
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0806136.8A external-priority patent/GB0806136D0/en
Priority claimed from GB0900848A external-priority patent/GB0900848D0/en
Application filed by Basf Se filed Critical Basf Se
Publication of CY1114785T1 publication Critical patent/CY1114785T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Μία μέθοδος για την ανίχνευση και την καταμέτρηση βιώσιμων μικροοργανισμών σε ένα δείγμα στο οποίο υπάρχει υποψία ότι περιέχει σχετικούς μικροοργανισμούς, περιλαμβάνει: (1) επαφή με το εν λόγω δείγμα με μια θρεπτική πηγή κυττάρων και έναν αναστολέα κυτταρικού πολλαπλασιασμού, με επαφή του εν λόγω δείγματος με τουλάχιστον έναν σημασμένο με φθορισμό ολιγονουκλεοτιδικό ανιχνευτή που είναι σε θέση να υποβάλει σε υβριδίωση εξειδικευμένα τουλάχιστον ένα μέρος του ριβοσώματος νουκλεϊνικών οξέων των εν λόγω μικροοργανισμών, (3) ανίχνευση και ποσοτικοποίηση του σήματος φθορισμού, στην οποία οι μικροοργανισμοί είναι του είδους Legionella pneumophila και στην οποία ο αναστολέας κυτταρικού πολλαπλασιασμού επιλέγεται από την ομάδα που αποτελείται από σιπροφλοξασίνη και κεφαλεξίνη. Η εφεύρεση περιλαμβάνει επίσης ένα κιτ που περιέχει: (1) μια θρεπτική πηγή κυττάρων, (2) έναν αναστολέα κυτταρικού πολλαπλασιασμού, (3) τουλάχιστον έναν σημασμένο με φθορισμό ολιγονουκλεοτιδικό ανιχνευτή που είναι σε θέση να υποβάλει σε υβριδίωση εξειδικευμένα τουλάχιστον ένα μέρος του ριβοσώματος νουκλεϊνικών οξέων των εν λόγω μικροοργανισμών, (4) ένα μέσο για την ανίχνευση και ποσοτικοποίηση του σήματος φθορισμού, στην οποία ο αναστολέας κυτταρικού πολλαπλασιασμού επιλέγεται από την ομάδα που αποτελείται από σιπροφλοξασίνη και κεφαλεξίνη.
CY20121100633T 2008-04-04 2012-07-17 Ανιχνευση και καταμετρηση μικροοργανισμων CY1114785T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0806136.8A GB0806136D0 (en) 2008-04-04 2008-04-04 Detection and enumeration of microorganisms
GB0900848A GB0900848D0 (en) 2009-01-20 2009-01-20 Detection and enumeration of microorganisms
EP09727970A EP2262910B1 (en) 2008-04-04 2009-03-20 Detection and enumeration of microorganisms

Publications (1)

Publication Number Publication Date
CY1114785T1 true CY1114785T1 (el) 2016-12-14

Family

ID=40887000

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20121100633T CY1114785T1 (el) 2008-04-04 2012-07-17 Ανιχνευση και καταμετρηση μικροοργανισμων

Country Status (18)

Country Link
US (1) US20110076686A1 (el)
EP (1) EP2262910B1 (el)
JP (1) JP5349579B2 (el)
KR (1) KR101265412B1 (el)
CN (1) CN101990579A (el)
AU (1) AU2009231362B2 (el)
CA (1) CA2719863C (el)
CY (1) CY1114785T1 (el)
DK (1) DK2262910T3 (el)
ES (1) ES2389861T3 (el)
HR (1) HRP20120553T1 (el)
IL (1) IL208158A0 (el)
MX (1) MX2010010385A (el)
MY (1) MY149655A (el)
PT (1) PT2262910E (el)
RS (1) RS52445B (el)
RU (1) RU2477319C2 (el)
WO (1) WO2009121727A1 (el)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2554470C2 (ru) * 2010-06-03 2015-06-27 Басф Се Обнаружение и подсчет микроорганизмов
CN105524974A (zh) * 2016-02-24 2016-04-27 北京世纪阿姆斯生物技术有限公司 一种高浓度微生物制剂产品含菌量的检测方法
CN107656056B (zh) * 2017-08-29 2019-05-28 山东师范大学 一种基于细菌增长对细菌快速镜检的方法
RU2699983C1 (ru) * 2018-12-04 2019-09-11 Федеральное бюджетное учреждение науки "Государственный научный центр прикладной микробиологии и биотехнологии" (ФБУН ГНЦ ПМБ) Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека Штамм гибридных культивируемых клеток животных Mus musculus 1F11PAL - продуцент мышиных моноклональных антител, специфичных к пептидогликан-ассоциированному липопротеину (PAL) Legionella pneumophila
EP3904530A1 (en) * 2020-04-27 2021-11-03 Universidade de Évora Method for collection, detection and/or identification of microorganisms from a natural stone and uses thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5834445A (en) * 1989-08-11 1998-11-10 Sikorski; Christopher Process for preparing decolorized carotenoid-cyclodextrin complexes
FR2845097B1 (fr) * 2002-10-01 2006-06-16 Metis Biotechnologies Procede de detection et de comptage de microorganismes dans un echantillon
JP4632839B2 (ja) * 2005-03-31 2011-02-16 アクアス株式会社 レジオネラ検査用選択培地、及び、レジオネラ属菌の培養方法
US20090239256A1 (en) * 2005-06-09 2009-09-24 South Australian Water Corporation Detection of Micro-Organisms
ATE531818T1 (de) * 2006-05-02 2011-11-15 Univ Paris Curie Methode zur bestimmung und auszählung von mikroorganismen

Also Published As

Publication number Publication date
MY149655A (en) 2013-09-30
RU2477319C2 (ru) 2013-03-10
MX2010010385A (es) 2010-12-15
JP2011516052A (ja) 2011-05-26
CN101990579A (zh) 2011-03-23
AU2009231362A1 (en) 2009-10-08
US20110076686A1 (en) 2011-03-31
RS52445B (en) 2013-02-28
AU2009231362B2 (en) 2012-07-05
ES2389861T3 (es) 2012-11-02
EP2262910B1 (en) 2012-06-06
IL208158A0 (en) 2010-12-30
KR20100134093A (ko) 2010-12-22
JP5349579B2 (ja) 2013-11-20
WO2009121727A1 (en) 2009-10-08
HRP20120553T1 (hr) 2012-10-31
EP2262910A1 (en) 2010-12-22
DK2262910T3 (da) 2012-09-17
RU2010144839A (ru) 2012-05-20
CA2719863C (en) 2013-10-08
PT2262910E (pt) 2012-07-18
KR101265412B1 (ko) 2013-05-16
CA2719863A1 (en) 2009-10-08

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