CY1112667T1 - Ενζυμικη αποκοπη των προδρομων μοριων ινσουλινης με μια παραλλαγη της θρυψινης - Google Patents

Ενζυμικη αποκοπη των προδρομων μοριων ινσουλινης με μια παραλλαγη της θρυψινης

Info

Publication number
CY1112667T1
CY1112667T1 CY20111101023T CY111101023T CY1112667T1 CY 1112667 T1 CY1112667 T1 CY 1112667T1 CY 20111101023 T CY20111101023 T CY 20111101023T CY 111101023 T CY111101023 T CY 111101023T CY 1112667 T1 CY1112667 T1 CY 1112667T1
Authority
CY
Cyprus
Prior art keywords
thrucine
cutoff
enzymatic
deletion
trypsin
Prior art date
Application number
CY20111101023T
Other languages
English (en)
Inventor
Frank Geipel
Stephan Glaser
Christoph Hoh
Erhard Kopetzki
Rainer Mueller
Frank Zocher
Original Assignee
Sanofi-Aventis Deutschland Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi-Aventis Deutschland Gmbh filed Critical Sanofi-Aventis Deutschland Gmbh
Publication of CY1112667T1 publication Critical patent/CY1112667T1/el

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Η παρούσα εφεύρεση αναφέρεται στη παραγωγή μιας παραλλαγής της ανασυνδυασμένης θρυψίνης με αυξημένη ειδικότητα υποστρώματος για αργινίνη έναντι της λυσίνης σε μη ζωικούς οργανισμούς ξενιστή. Επιπλέον, η παρούσα εφεύρεση αναφέρεται σε μια παραλλαγή ανασυνδυασμένης θρυψίνης και τη παραγωγή τους. Επίσης παρέχεται η χρήση παραλλαγών ανασυνδυασμένης χοίρειας παγκρεατικής θρυψίνης για αποκοπή πρόδρομων μορίων ινσουλινών, και κιτ που περιέχουν τη παραλλαγή της θρυψίνης.
CY20111101023T 2005-09-14 2011-10-27 Ενζυμικη αποκοπη των προδρομων μοριων ινσουλινης με μια παραλλαγη της θρυψινης CY1112667T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05077086 2005-09-14
EP06791678A EP1926749B1 (en) 2005-09-14 2006-08-26 Cleavage of precursors of insulins by a variant of trypsin

Publications (1)

Publication Number Publication Date
CY1112667T1 true CY1112667T1 (el) 2016-02-10

Family

ID=36190640

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20111101023T CY1112667T1 (el) 2005-09-14 2011-10-27 Ενζυμικη αποκοπη των προδρομων μοριων ινσουλινης με μια παραλλαγη της θρυψινης

Country Status (20)

Country Link
US (1) US7981635B2 (el)
EP (1) EP1926749B1 (el)
JP (1) JP5027812B2 (el)
KR (1) KR101304958B1 (el)
CN (1) CN101253196B (el)
AR (1) AR055428A1 (el)
AT (1) ATE517919T1 (el)
AU (1) AU2006291780B2 (el)
BR (1) BRPI0616054B8 (el)
CA (1) CA2621344C (el)
CY (1) CY1112667T1 (el)
ES (1) ES2370657T3 (el)
HK (1) HK1124341A1 (el)
IL (1) IL189887A (el)
MX (1) MX2008003362A (el)
MY (1) MY146082A (el)
PL (1) PL1926749T3 (el)
PT (1) PT1926749E (el)
SI (1) SI1926749T1 (el)
WO (1) WO2007031187A1 (el)

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CN102994600A (zh) * 2012-12-11 2013-03-27 鲁南新时代生物技术有限公司 甘精胰岛素前体的酶切转化方法
HUE062573T2 (hu) 2014-12-12 2023-11-28 Sanofi Aventis Deutschland Glargin inzulin/lixiszenatid rögzített arányú készítmény
TWI748945B (zh) 2015-03-13 2021-12-11 德商賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患治療
TW201705975A (zh) 2015-03-18 2017-02-16 賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患之治療
CN108018277A (zh) * 2016-11-04 2018-05-11 江苏万邦生化医药股份有限公司 重组牛胰蛋白酶突变体及其制备方法
KR20190036956A (ko) 2017-09-28 2019-04-05 한미약품 주식회사 지속형 단쇄 인슐린 아날로그 및 이의 결합체
US11518988B2 (en) * 2018-02-22 2022-12-06 Sanofi-Aventis Deutschland Gmbh Variants of porcine trypsin
EP3650541A1 (en) * 2018-11-08 2020-05-13 BioPharma Translationsinstitut Dessau Forschungs GmbH Trypsin mutant for c- and n-terminal modification of polypeptides
KR20200080747A (ko) * 2018-12-27 2020-07-07 주식회사 폴루스 인슐린 전구체의 인슐린 효소 전환용 조성물 및 이를 이용하여 인슐린 전구체를 인슐린으로 전환하는 방법
IL293635A (en) 2019-12-10 2022-08-01 Sanofi Sa A method for preparing a conjugate of a sulfonamide and a polypeptide
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Also Published As

Publication number Publication date
MY146082A (en) 2012-06-29
JP5027812B2 (ja) 2012-09-19
BRPI0616054B8 (pt) 2021-05-25
CA2621344A1 (en) 2007-03-22
AR055428A1 (es) 2007-08-22
US20100196953A1 (en) 2010-08-05
PT1926749E (pt) 2011-09-29
US7981635B2 (en) 2011-07-19
CA2621344C (en) 2014-12-16
EP1926749A1 (en) 2008-06-04
PL1926749T3 (pl) 2011-12-30
AU2006291780A1 (en) 2007-03-22
AU2006291780B2 (en) 2011-12-08
EP1926749B1 (en) 2011-07-27
ES2370657T3 (es) 2011-12-21
BRPI0616054B1 (pt) 2019-08-06
JP2009507500A (ja) 2009-02-26
ATE517919T1 (de) 2011-08-15
SI1926749T1 (sl) 2011-11-30
CN101253196B (zh) 2012-09-05
WO2007031187A1 (en) 2007-03-22
BRPI0616054A2 (pt) 2011-06-07
HK1124341A1 (en) 2009-07-10
IL189887A (en) 2012-02-29
WO2007031187A8 (en) 2007-08-16
MX2008003362A (es) 2008-03-25
CN101253196A (zh) 2008-08-27
KR20080055843A (ko) 2008-06-19
IL189887A0 (en) 2008-11-03
KR101304958B1 (ko) 2013-09-17

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