AR067924A1 - Procedimiento de purificacion o de deteccion de una proteina diana - Google Patents

Procedimiento de purificacion o de deteccion de una proteina diana

Info

Publication number
AR067924A1
AR067924A1 ARP080103523A ARP080103523A AR067924A1 AR 067924 A1 AR067924 A1 AR 067924A1 AR P080103523 A ARP080103523 A AR P080103523A AR P080103523 A ARP080103523 A AR P080103523A AR 067924 A1 AR067924 A1 AR 067924A1
Authority
AR
Argentina
Prior art keywords
factor
protein
detection
target protein
purification
Prior art date
Application number
ARP080103523A
Other languages
English (en)
Inventor
Laurent Siret
Abdessatar Chtourou
Frederic Dhainaut
Gerald Perret
Original Assignee
Lfb Biotechnologies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lfb Biotechnologies filed Critical Lfb Biotechnologies
Publication of AR067924A1 publication Critical patent/AR067924A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/745—Assays involving non-enzymic blood coagulation factors
    • G01N2333/755—Factors VIII, e.g. factor VIII C [AHF], factor VIII Ag [VWF]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974—Thrombin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Procedimiento para la purificacion o la deteccion de una proteína diana presente en una solucion, comprendiendo dicho procedimiento, previamente a la realizacion de la etapa de deteccion o de purificacion propiamente dicha, una etapa de puesta en contacto de dicha solucion con un aptámero ADN inmovilizado sobre un soporte solido, que se liga específicamente con dicha proteína diana, no ligándose dicho aptámero con una proteína homologa de dicha proteína diana susceptible de estar también presente en solucion. Reivindicacion 4: Procedimiento segun cualquiera de las reivindicaciones 1 a 3, caracterizado porque dicha proteína diana es un factor de la coagulacion seleccionado de entre el factor VII (FVII), el factor VIII (FVIII), el factor X (FX), el factor IX (Factor IX), el factor XI (FXI), el factor XII (FXII), el factor XIII (FXIII), el factor II (TROMBINA), la antitrombina III (AT III), el cofactor II de la heparina (HCII), la proteína C (PC), la trombomodulina (TM), la proteína S (PS), el factor V (FV), el factor de Willerbrand (FvW) y el inhibidor de la ví del factor tisular (TFPI).
ARP080103523A 2007-08-14 2008-08-13 Procedimiento de purificacion o de deteccion de una proteina diana AR067924A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0757072A FR2920024B1 (fr) 2007-08-14 2007-08-14 Procede de purification ou de detection d'une proteine cible

Publications (1)

Publication Number Publication Date
AR067924A1 true AR067924A1 (es) 2009-10-28

Family

ID=39321464

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP080103523A AR067924A1 (es) 2007-08-14 2008-08-13 Procedimiento de purificacion o de deteccion de una proteina diana

Country Status (12)

Country Link
US (2) US9175034B2 (es)
EP (2) EP2178901B1 (es)
JP (2) JP6006472B2 (es)
KR (2) KR20160052796A (es)
CN (2) CN101821282A (es)
AR (1) AR067924A1 (es)
AU (1) AU2008290416B2 (es)
BR (1) BRPI0815410A2 (es)
CA (1) CA2696363A1 (es)
ES (2) ES2636453T3 (es)
FR (1) FR2920024B1 (es)
WO (1) WO2009024726A2 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2942231B1 (fr) * 2009-02-19 2015-03-20 Lfb Biotechnologies Acides nucleiques se liant specifiquement au facteur vii/viia humain, et utilisations
FR2942232B1 (fr) * 2009-02-19 2015-03-13 Lfb Biotechnologies Moyens pour la purification d'une proteine de la coagulation et procedes pour sa mise en oeuvre
JP5691161B2 (ja) * 2009-11-19 2015-04-01 Jnc株式会社 タンパク質の精製方法
US8785132B2 (en) * 2010-04-23 2014-07-22 Postech Academy-Industry Foundation Aptamer sandwich assays
JP6178727B2 (ja) * 2010-12-30 2017-08-09 ラボラトワール フランセ デュ フラクショヌマン エ デ ビオテクノロジ 核酸リガンドを固定する方法
FR2981651B1 (fr) * 2011-10-24 2015-06-19 Lfb Biotechnologies Procede d'immobilisation de ligands nucleiques.
CN102965378B (zh) * 2012-11-14 2014-10-15 广西安仁欣生物科技有限公司 一种糖化血红蛋白的核酸适体及其制备方法
CN104109675B (zh) * 2012-11-14 2016-07-06 广西安仁欣生物科技有限公司 一种糖化血红蛋白的核酸适体及其制备方法
CN104164436B (zh) * 2012-11-14 2016-10-05 广西安仁欣生物科技有限公司 一种糖化血红蛋白的核酸适体及其制备方法
CN104109674B (zh) * 2012-11-14 2016-07-06 广西安仁欣生物科技有限公司 一种糖化血红蛋白的核酸适体及其制备方法
JP6646310B2 (ja) * 2015-05-08 2020-02-14 学校法人日本大学 標識ペプチドおよびその使用
FR3054039B1 (fr) * 2016-07-12 2020-05-29 Universite De Montpellier Biocapteur pour detecter la presence de bacteries
JP2021530230A (ja) * 2018-07-17 2021-11-11 ユニヴァーシティ オブ ワシントン アプタマーベースの可逆的細胞選択に関連する組成物および方法
US11421234B2 (en) * 2019-12-02 2022-08-23 Chang Gung University Aptamers for targeting coagulation factor XIII and uses thereof

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0264166B1 (en) 1986-04-09 1996-08-21 Genzyme Corporation Transgenic animals secreting desired proteins into milk
ATE160821T1 (de) 1990-06-11 1997-12-15 Nexstar Pharmaceuticals Inc Nukleinsäureliganden
US5965789A (en) * 1991-01-11 1999-10-12 American Red Cross Engineering protein posttranslational modification by PACE/furin in transgenic non-human mammals
FR2677652B1 (fr) 1991-06-12 2005-05-27 Agronomique Inst Nat Rech Procede de preparation d'une proteine d'interet dans le lait d'un animal transgenique, produit obtenu et cellule eucaryote utilisee.
US5288853A (en) * 1992-04-30 1994-02-22 Alpha Therapeutic Corporation Factor viii purification process
WO1994008050A1 (en) 1992-09-29 1994-04-14 Nexagen, Inc. Nucleic acid ligands and methods for producing the same
US5880327A (en) 1994-09-21 1999-03-09 American National Red Cross Transgenic mammals expressing human coagulation factor VIII
JPH11509404A (ja) * 1995-05-04 1999-08-24 アメリカン レッド クロス トランスジェニック動物における、蛋白質の翻訳後修飾の作出
WO1997001755A2 (en) * 1995-06-26 1997-01-16 Perseptive Biosystems, Inc. High speed, automated, continuous flow, multi-dimensional molecular selection and analysis
US20060057573A1 (en) * 2002-02-15 2006-03-16 Somalogic, Inc Methods and reagents for detecting target binding by nucleic acid ligands
CA2425208C (en) * 2000-09-26 2013-04-09 Duke University Rna aptamers and methods for identifying the same
EP1730158A2 (en) * 2003-08-06 2006-12-13 Cygene, Inc. Methods and compositions for in vitro and in vivo use of parallel stranded hairpins and triplex structures as nucleic acid ligands
US7329742B2 (en) * 2003-09-04 2008-02-12 The Regents Of The University Of California Aptamers and methods for their in vitro selection and uses thereof
AU2005333126A1 (en) * 2004-07-18 2006-12-21 Csl Limited Methods and compositions for inducing innate immune responses
EP1918372A4 (en) * 2005-07-05 2009-08-12 Ribomic Inc NUCLEIC ACID CAPABLE OF BINDING TO IMMUNOGLOBULIN G AND USE THEREOF

Also Published As

Publication number Publication date
US20110097715A1 (en) 2011-04-28
EP2508533B1 (fr) 2016-08-03
WO2009024726A2 (fr) 2009-02-26
US9175034B2 (en) 2015-11-03
KR20100058479A (ko) 2010-06-03
CA2696363A1 (fr) 2009-02-26
BRPI0815410A2 (pt) 2015-02-03
FR2920024A1 (fr) 2009-02-20
CN105859829A (zh) 2016-08-17
EP2178901A2 (fr) 2010-04-28
EP2178901B1 (fr) 2017-05-10
CN101821282A (zh) 2010-09-01
US20160009761A1 (en) 2016-01-14
EP2508533A1 (fr) 2012-10-10
ES2636453T3 (es) 2017-10-05
WO2009024726A3 (fr) 2009-04-30
JP2016117746A (ja) 2016-06-30
KR20160052796A (ko) 2016-05-12
FR2920024B1 (fr) 2012-12-14
JP6006472B2 (ja) 2016-10-12
AU2008290416A1 (en) 2009-02-26
ES2599303T3 (es) 2017-02-01
AU2008290416B2 (en) 2013-11-21
JP2010535853A (ja) 2010-11-25

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