CY1110431T1 - Μεθοδος παραγωγης περιοχων λειτουργικων πρωτεϊνων - Google Patents

Μεθοδος παραγωγης περιοχων λειτουργικων πρωτεϊνων

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Publication number
CY1110431T1
CY1110431T1 CY20081100728T CY081100728T CY1110431T1 CY 1110431 T1 CY1110431 T1 CY 1110431T1 CY 20081100728 T CY20081100728 T CY 20081100728T CY 081100728 T CY081100728 T CY 081100728T CY 1110431 T1 CY1110431 T1 CY 1110431T1
Authority
CY
Cyprus
Prior art keywords
protein domains
exon sequences
producing operating
protein areas
primary
Prior art date
Application number
CY20081100728T
Other languages
English (en)
Inventor
Luca Giampiero De
Luca Falciola
Original Assignee
Laboratoires Serono Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Laboratoires Serono Sa filed Critical Laboratoires Serono Sa
Publication of CY1110431T1 publication Critical patent/CY1110431T1/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Η παρούσα εφεύρεση παρέχει μία μέθοδο παραγωγής περιοχών λειτουργικών πρωτεϊνών οι οποίες είναι θραύσματα πρωτογενών μεταφραστικών προϊόντων που συνίστανται εκ μιας ή περισσοτέρων διακριτών περιοχών πρωτεΐνης που κωδικοποιούνται μέσω συγκεκριμένων υποσυνόλων εξονικών ακολουθιών. Η μέθοδος βασίζεται στην ενσωμάτωση μέσω ενός μονού συμβάματος ομόλογου ανασυνδυασμού, μιας ρυθμιστικής μονάδας μέσα στο ευκαρυωτικό γονίδιο που κωδικοποιεί το πρωτογενές μεταφραστικό προϊόν στο επίπεδο της ιντρονικής γονιδιωματικής περιοχής άμεσα και παρακείμενα στις εξονικές ακολουθίες, οι οποίες κωδικοποιούν την περιοχή λειτουργικής πρωτεΐνης.
CY20081100728T 2000-08-01 2008-07-11 Μεθοδος παραγωγης περιοχων λειτουργικων πρωτεϊνων CY1110431T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0018876.3A GB0018876D0 (en) 2000-08-01 2000-08-01 Method of producing polypeptides
EP01953251A EP1305411B1 (en) 2000-08-01 2001-08-01 Method of producing functional protein domains

Publications (1)

Publication Number Publication Date
CY1110431T1 true CY1110431T1 (el) 2015-04-29

Family

ID=9896767

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20081100728T CY1110431T1 (el) 2000-08-01 2008-07-11 Μεθοδος παραγωγης περιοχων λειτουργικων πρωτεϊνων

Country Status (21)

Country Link
US (1) US8048645B2 (el)
EP (1) EP1305411B1 (el)
JP (1) JP5068916B2 (el)
CN (1) CN1468304B (el)
AR (1) AR031717A1 (el)
AT (1) ATE398174T1 (el)
AU (1) AU7573901A (el)
BR (1) BR0112979A (el)
CA (1) CA2415768C (el)
CY (1) CY1110431T1 (el)
DE (1) DE60134410D1 (el)
DK (1) DK1305411T3 (el)
ES (1) ES2305091T3 (el)
GB (1) GB0018876D0 (el)
HK (1) HK1059281A1 (el)
IL (1) IL154212A0 (el)
NO (1) NO330897B1 (el)
PT (1) PT1305411E (el)
SI (1) SI1305411T1 (el)
WO (1) WO2002010372A1 (el)
ZA (1) ZA200300827B (el)

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Also Published As

Publication number Publication date
CA2415768A1 (en) 2002-02-07
NO20030443L (no) 2003-03-25
EP1305411A1 (en) 2003-05-02
EP1305411B1 (en) 2008-06-11
DK1305411T3 (da) 2008-09-01
AR031717A1 (es) 2003-10-01
SI1305411T1 (sl) 2008-10-31
DE60134410D1 (de) 2008-07-24
NO330897B1 (no) 2011-08-08
WO2002010372A1 (en) 2002-02-07
ATE398174T1 (de) 2008-07-15
CN1468304B (zh) 2011-01-19
WO2002010372A8 (en) 2002-02-28
IL154212A0 (en) 2003-07-31
CA2415768C (en) 2011-05-17
JP5068916B2 (ja) 2012-11-07
BR0112979A (pt) 2003-07-08
ZA200300827B (en) 2004-02-25
US20040053362A1 (en) 2004-03-18
JP2004504840A (ja) 2004-02-19
HK1059281A1 (en) 2004-06-25
CN1468304A (zh) 2004-01-14
US8048645B2 (en) 2011-11-01
AU7573901A (en) 2002-02-13
ES2305091T3 (es) 2008-11-01
GB0018876D0 (en) 2000-09-20
NO20030443D0 (no) 2003-01-28
PT1305411E (pt) 2008-07-03

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