AR031717A1 - Metodo para producir una proteina que es dominio de una proteina que es una proteina funcional; proceso para expresar selectivamente exones que codifican dicha proteina; gen recombinante integrado en el gen de celulas eucariotas, que comprende dicho exon; constructos de dna para producir dichas prot - Google Patents

Metodo para producir una proteina que es dominio de una proteina que es una proteina funcional; proceso para expresar selectivamente exones que codifican dicha proteina; gen recombinante integrado en el gen de celulas eucariotas, que comprende dicho exon; constructos de dna para producir dichas prot

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Publication number
AR031717A1
AR031717A1 ARP010103647A ARP010103647A AR031717A1 AR 031717 A1 AR031717 A1 AR 031717A1 AR P010103647 A ARP010103647 A AR P010103647A AR P010103647 A ARP010103647 A AR P010103647A AR 031717 A1 AR031717 A1 AR 031717A1
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AR
Argentina
Prior art keywords
protein
gen
domain
producing
eucariot
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ARP010103647A
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English (en)
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Applied Research Systems
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Publication date
Application filed by Applied Research Systems filed Critical Applied Research Systems
Publication of AR031717A1 publication Critical patent/AR031717A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se describe y reivindica un método para producir dominios proteicos funcionales que son fragmentos de productos primarios de la traduccion, que comprenden al menos un dominio proteico codificado por subgrupos de secuencias exonicas específicas. El método está basado en la integracion recombinante homologa de una unidad reguladora en el gen eucariotico que codifica el producto de la traduccion primaria; a nivel de la region genomica intronica, inmediatamente adyacente a las secuencias exonicas que codifican dichos dominios proteicos funcionales. Específicamente se trata de obtener una proteína que es el dominio de una proteína funcional, indistintamente C-terminal o N-terminal, proveniente de la traduccion primaria de un gen. Se describen y reivindican además los objetos restantes mencionados en el título.
ARP010103647A 2000-08-01 2001-07-31 Metodo para producir una proteina que es dominio de una proteina que es una proteina funcional; proceso para expresar selectivamente exones que codifican dicha proteina; gen recombinante integrado en el gen de celulas eucariotas, que comprende dicho exon; constructos de dna para producir dichas prot AR031717A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0018876.3A GB0018876D0 (en) 2000-08-01 2000-08-01 Method of producing polypeptides

Publications (1)

Publication Number Publication Date
AR031717A1 true AR031717A1 (es) 2003-10-01

Family

ID=9896767

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP010103647A AR031717A1 (es) 2000-08-01 2001-07-31 Metodo para producir una proteina que es dominio de una proteina que es una proteina funcional; proceso para expresar selectivamente exones que codifican dicha proteina; gen recombinante integrado en el gen de celulas eucariotas, que comprende dicho exon; constructos de dna para producir dichas prot

Country Status (21)

Country Link
US (1) US8048645B2 (es)
EP (1) EP1305411B1 (es)
JP (1) JP5068916B2 (es)
CN (1) CN1468304B (es)
AR (1) AR031717A1 (es)
AT (1) ATE398174T1 (es)
AU (1) AU7573901A (es)
BR (1) BR0112979A (es)
CA (1) CA2415768C (es)
CY (1) CY1110431T1 (es)
DE (1) DE60134410D1 (es)
DK (1) DK1305411T3 (es)
ES (1) ES2305091T3 (es)
GB (1) GB0018876D0 (es)
HK (1) HK1059281A1 (es)
IL (1) IL154212A0 (es)
NO (1) NO330897B1 (es)
PT (1) PT1305411E (es)
SI (1) SI1305411T1 (es)
WO (1) WO2002010372A1 (es)
ZA (1) ZA200300827B (es)

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Also Published As

Publication number Publication date
EP1305411B1 (en) 2008-06-11
DK1305411T3 (da) 2008-09-01
EP1305411A1 (en) 2003-05-02
WO2002010372A8 (en) 2002-02-28
GB0018876D0 (en) 2000-09-20
CY1110431T1 (el) 2015-04-29
CA2415768C (en) 2011-05-17
BR0112979A (pt) 2003-07-08
ATE398174T1 (de) 2008-07-15
NO330897B1 (no) 2011-08-08
ZA200300827B (en) 2004-02-25
JP2004504840A (ja) 2004-02-19
PT1305411E (pt) 2008-07-03
US20040053362A1 (en) 2004-03-18
DE60134410D1 (de) 2008-07-24
WO2002010372A1 (en) 2002-02-07
ES2305091T3 (es) 2008-11-01
HK1059281A1 (en) 2004-06-25
CN1468304A (zh) 2004-01-14
US8048645B2 (en) 2011-11-01
AU7573901A (en) 2002-02-13
NO20030443L (no) 2003-03-25
NO20030443D0 (no) 2003-01-28
CN1468304B (zh) 2011-01-19
CA2415768A1 (en) 2002-02-07
SI1305411T1 (sl) 2008-10-31
JP5068916B2 (ja) 2012-11-07
IL154212A0 (en) 2003-07-31

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