DE69939056D1 - Vektoren für genmutagenese und genentdeckung - Google Patents

Vektoren für genmutagenese und genentdeckung

Info

Publication number
DE69939056D1
DE69939056D1 DE69939056T DE69939056T DE69939056D1 DE 69939056 D1 DE69939056 D1 DE 69939056D1 DE 69939056 T DE69939056 T DE 69939056T DE 69939056 T DE69939056 T DE 69939056T DE 69939056 D1 DE69939056 D1 DE 69939056D1
Authority
DE
Germany
Prior art keywords
vectors
gene
gene mutagenesis
novel
genetic coverage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69939056T
Other languages
English (en)
Inventor
Brian Zambrowicz
Glenn A Friedrich
Arthur T Sands
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lexicon Pharmaceuticals Inc
Original Assignee
Lexicon Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/057,328 external-priority patent/US6080576A/en
Application filed by Lexicon Pharmaceuticals Inc filed Critical Lexicon Pharmaceuticals Inc
Application granted granted Critical
Publication of DE69939056D1 publication Critical patent/DE69939056D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/60—Vectors containing traps for, e.g. exons, promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69939056T 1998-03-27 1999-03-26 Vektoren für genmutagenese und genentdeckung Expired - Lifetime DE69939056D1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US7972998P 1998-03-27 1998-03-27
US09/057,328 US6080576A (en) 1998-03-27 1998-04-08 Vectors for gene trapping and gene activation
US8172798P 1998-04-14 1998-04-14
PCT/US1999/006474 WO1999050426A1 (en) 1998-03-27 1999-03-26 Vectors for gene mutagenesis and gene discovery

Publications (1)

Publication Number Publication Date
DE69939056D1 true DE69939056D1 (de) 2008-08-21

Family

ID=27369230

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69939056T Expired - Lifetime DE69939056D1 (de) 1998-03-27 1999-03-26 Vektoren für genmutagenese und genentdeckung

Country Status (8)

Country Link
EP (1) EP1066392B1 (de)
JP (1) JP3725782B2 (de)
AT (1) ATE400660T1 (de)
AU (1) AU751520B2 (de)
CA (1) CA2323834C (de)
DE (1) DE69939056D1 (de)
DK (1) DK1066392T3 (de)
WO (1) WO1999050426A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7332338B2 (en) 1996-10-04 2008-02-19 Lexicon Pharmaceuticals, Inc. Vectors for making genomic modifications
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US7316923B1 (en) 1997-09-26 2008-01-08 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US6436707B1 (en) * 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
AU2004203361B2 (en) * 1998-03-27 2007-05-10 Lexicon Pharmaceuticals, Inc. Vectors for gene mutagenesis and gene discovery by gene trapping
AU772677B2 (en) * 1998-03-27 2004-05-06 Lexicon Pharmaceuticals, Inc. Vectors for gene mutagenesis and gene discovery
US6808921B1 (en) 1998-03-27 2004-10-26 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
CA2339041A1 (en) 1998-08-10 2000-02-24 Lexicon Genetics Incorporated Construction of normalized cdna libraries from eucaryotic cells
EP1584689A1 (de) * 1998-11-20 2005-10-12 Lexicon Genetics Incorporated Vektoren für die Gen-Mutagenese und die Entdeckung von Genen
WO2000049162A2 (en) * 1999-02-19 2000-08-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
KR20020057943A (ko) 1999-07-14 2002-07-12 가부시끼가이샤 트랜스제닉 트랩 벡터 및 이를 이용한 유전자 트랩법
EP1092768A1 (de) * 1999-10-16 2001-04-18 ARTEMIS Pharmaceuticals GmbH Konstrukt zum bedingten einfangen und Disruption von Genen
US6897020B2 (en) 2000-03-20 2005-05-24 Newlink Genetics Inc. Methods and compositions for elucidating relative protein expression levels in cells
CA2403567A1 (en) * 2000-03-20 2001-09-27 Newlink Genetics Methods and compositions for elucidating protein expression profiles in cells
GB0018876D0 (en) 2000-08-01 2000-09-20 Applied Research Systems Method of producing polypeptides
AU2002219841A1 (en) 2000-11-16 2002-05-27 Cornell Research Foundation Inc. Vectors for conditional gene inactivation
WO2002053732A2 (en) * 2000-12-28 2002-07-11 Pangenex Methods for making polynucleotide libraries, polynucleotide arrays, and cell librraries for high-throughput genomics analysis
ES2312607T3 (es) * 2001-03-28 2009-03-01 Dana Farber Cancer Inst Inc Identificacion y caracterizacion de genes.
EP1444326A4 (de) * 2001-08-24 2006-06-28 Advanced Cell Tech Inc Screening-assays zur identifizierung differenzierung induzierender stoffe und herstellung differenzierter zellen zur zelltherapie
US20040091936A1 (en) 2002-05-24 2004-05-13 Michael West Bank of stem cells for producing cells for transplantation having HLA antigens matching those of transplant recipients, and methods for making and using such a stem cell bank
US7205148B2 (en) 2003-06-11 2007-04-17 Regeneron Pharmaceuticals, Inc. Genome mutation by intron insertion into an embryonic stem cell genome
KR100747381B1 (ko) * 2006-01-20 2007-08-07 미쓰비시덴키 가부시키가이샤 엘리베이터의 그룹 관리 제어 장치
JP4867012B2 (ja) * 2007-03-22 2012-02-01 国立大学法人群馬大学 レトロウイルス産生用ベクター
EP2318527A4 (de) * 2008-07-30 2012-07-04 Michael Strathmann Gen-targeting-verfahren
CN112824529B (zh) * 2019-11-21 2022-09-09 北京大学 一种条件性基因敲除或者拯救方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4085297A (en) * 1996-08-23 1998-03-06 Chiron Corporation Human polyhomeotic 1(hphl) acts as a tumor suppressor
US6207371B1 (en) * 1996-10-04 2001-03-27 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same

Also Published As

Publication number Publication date
CA2323834A1 (en) 1999-10-07
CA2323834C (en) 2009-01-27
EP1066392B1 (de) 2008-07-09
AU751520B2 (en) 2002-08-15
DK1066392T3 (da) 2008-08-25
WO1999050426A1 (en) 1999-10-07
EP1066392A1 (de) 2001-01-10
ATE400660T1 (de) 2008-07-15
JP3725782B2 (ja) 2005-12-14
AU3203699A (en) 1999-10-18
JP2002509727A (ja) 2002-04-02

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