DE69836430D1 - Mikrobielle gene die für ausgeschiedene beta-glucuronidasen kodieren, ihre genprodukte und deren verwendungen - Google Patents

Mikrobielle gene die für ausgeschiedene beta-glucuronidasen kodieren, ihre genprodukte und deren verwendungen

Info

Publication number
DE69836430D1
DE69836430D1 DE69836430T DE69836430T DE69836430D1 DE 69836430 D1 DE69836430 D1 DE 69836430D1 DE 69836430 T DE69836430 T DE 69836430T DE 69836430 T DE69836430 T DE 69836430T DE 69836430 D1 DE69836430 D1 DE 69836430D1
Authority
DE
Germany
Prior art keywords
beta
glucuronidases
decayed
gen
code
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69836430T
Other languages
English (en)
Inventor
A Jefferson
Andrzej Kilian
Konrad Keese
Joanna Wilson
Louise Harcourt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Center for Application of Molecular Biology to International Agriculture
Original Assignee
Center for Application of Molecular Biology to International Agriculture
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Center for Application of Molecular Biology to International Agriculture filed Critical Center for Application of Molecular Biology to International Agriculture
Application granted granted Critical
Publication of DE69836430D1 publication Critical patent/DE69836430D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01031Beta-glucuronidase (3.2.1.31)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
DE69836430T 1997-09-09 1998-09-09 Mikrobielle gene die für ausgeschiedene beta-glucuronidasen kodieren, ihre genprodukte und deren verwendungen Expired - Lifetime DE69836430D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5826397P 1997-09-09 1997-09-09
PCT/US1998/019217 WO1999013085A2 (en) 1997-09-09 1998-09-09 MICROBIAL GENES FOR SECRETED β-GLUCURONIDASES, GENE PRODUCTS AND USES THEREOF

Publications (1)

Publication Number Publication Date
DE69836430D1 true DE69836430D1 (de) 2006-12-28

Family

ID=22015715

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69836430T Expired - Lifetime DE69836430D1 (de) 1997-09-09 1998-09-09 Mikrobielle gene die für ausgeschiedene beta-glucuronidasen kodieren, ihre genprodukte und deren verwendungen

Country Status (11)

Country Link
US (2) US6391547B1 (de)
EP (1) EP1012294B1 (de)
JP (1) JP2001515724A (de)
AT (1) ATE345388T1 (de)
AU (1) AU760275B2 (de)
BR (1) BR9811748A (de)
CA (1) CA2303423A1 (de)
DE (1) DE69836430D1 (de)
IL (2) IL134700A0 (de)
NZ (1) NZ503020A (de)
WO (1) WO1999013085A2 (de)

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US6641996B1 (en) * 1997-09-09 2003-11-04 Cambia Microbial β-glucuronidase genes, gene products and uses thereof
US6391547B1 (en) 1997-09-09 2002-05-21 Center For The Application Of Molecular Biology To International Agriculture Microbial β-glucuronidase genes, gene products and uses thereof
WO2003003816A2 (en) * 2001-07-06 2003-01-16 Monsanto Technology Llc Methods for enhancing segregation of transgenes in plants and compositions thereof
FR2877014B1 (fr) * 2004-10-21 2009-07-10 Inst Rech Pour Le Dev I R D Et Systemes d'expression de proteines recombinantes et leurs applications
NZ564025A (en) 2005-05-11 2012-03-30 Vector Tobacco Inc Reduced risk tobacco products and methods of making same
US9024111B1 (en) * 2011-03-31 2015-05-05 Sandia Corporation Methods and materials for deconstruction of biomass for biofuels production
US9719075B2 (en) 2014-09-29 2017-08-01 Integrated Micro-Chromatography Systems Mutant Staphylococcus beta-glucuronidase enzymes with enhanced enzymatic activity
US9920306B2 (en) 2014-09-29 2018-03-20 Integrated Micro-Chromatography Systems, Llc Mutant β-glucuronidase enzymes with enhanced enzymatic activity
US9909111B2 (en) 2016-03-21 2018-03-06 Integrated Micro-Chromatography Systems Mutant lactobacillus beta-glucuronidase enzymes with enhanced enzymatic activity
US20200024586A1 (en) 2017-01-20 2020-01-23 Integrated Micro-Chromatography Systems, Inc. Thermostabile beta-glucuronidase formulations
US11268079B2 (en) 2018-08-01 2022-03-08 Integrated Micro-Chromatography Systems, Inc. Compositions of beta-glucuronidase enzyme blends with enhanced enzymatic activity and methods of preparation thereof
US11421210B2 (en) 2018-10-08 2022-08-23 Integrated Micro-Chromatography Systems, Inc. Chimeric and other variant beta-glucuronidase enzymes with enhanced properties

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Also Published As

Publication number Publication date
JP2001515724A (ja) 2001-09-25
EP1012294B1 (de) 2006-11-15
ATE345388T1 (de) 2006-12-15
US7141719B2 (en) 2006-11-28
AU760275B2 (en) 2003-05-08
BR9811748A (pt) 2001-11-27
IL134700A0 (en) 2001-04-30
IL134700A (en) 2007-05-15
US20030157684A1 (en) 2003-08-21
NZ503020A (en) 2003-03-28
WO1999013085A2 (en) 1999-03-18
EP1012294A2 (de) 2000-06-28
CA2303423A1 (en) 1999-03-18
WO1999013085A3 (en) 1999-08-05
AU9485798A (en) 1999-03-29
US6391547B1 (en) 2002-05-21

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