CU22683A1 - Epítopes de la proteína pre-m/m del virus del dengue, péptidos sintéticos, proteínas quiméricas y sus usos - Google Patents
Epítopes de la proteína pre-m/m del virus del dengue, péptidos sintéticos, proteínas quiméricas y sus usosInfo
- Publication number
- CU22683A1 CU22683A1 CU1997013A CU1997013A CU22683A1 CU 22683 A1 CU22683 A1 CU 22683A1 CU 1997013 A CU1997013 A CU 1997013A CU 1997013 A CU1997013 A CU 1997013A CU 22683 A1 CU22683 A1 CU 22683A1
- Authority
- CU
- Cuba
- Prior art keywords
- protein
- peptides
- dengue
- virus
- synthetic peptides
- Prior art date
Links
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 6
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 6
- 241000725619 Dengue virus Species 0.000 title abstract 3
- 102000004169 proteins and genes Human genes 0.000 title 2
- 108090000623 proteins and genes Proteins 0.000 title 2
- 241000710815 Dengue virus 2 Species 0.000 abstract 2
- 101710085938 Matrix protein Proteins 0.000 abstract 2
- 101710127721 Membrane protein Proteins 0.000 abstract 2
- 230000003053 immunization Effects 0.000 abstract 2
- 238000002649 immunization Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 102000014914 Carrier Proteins Human genes 0.000 abstract 1
- 108010078791 Carrier Proteins Proteins 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 241000710831 Flavivirus Species 0.000 abstract 1
- 241000699670 Mus sp. Species 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 238000003745 diagnosis Methods 0.000 abstract 1
- 230000004927 fusion Effects 0.000 abstract 1
- 102000037865 fusion proteins Human genes 0.000 abstract 1
- 108020001507 fusion proteins Proteins 0.000 abstract 1
- 230000028993 immune response Effects 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 238000006386 neutralization reaction Methods 0.000 abstract 1
- 230000003472 neutralizing effect Effects 0.000 abstract 1
- 230000000405 serological effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
- C12N2770/24122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/18—Togaviridae; Flaviviridae
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
La presente invención está relacionada con cinco péptidos sintéticos pertenecientes a la proteína pre-M del virus Dengue-2 (3-31, 45-67, 57-92, 69-93, 103-124), los cuales fueron denominados B 19-6, B 20-2, B 19-5, B 20-1 y B 20-3 respectivamente que cubren el 58% de su secuencia aminocídica (97/166 AA), fueron sintetizados químicamente. Dos de los péptidos (19-6 y 20-3) fueron conjugados a una proteína carrier. Los anticuerpos obtenidos tras la inmunización de ratones Balb/c con dichos conjugados fueron evaluados mediante la técnica de neutralización in vitro por reducción del número de placas. También se construyeron proteínas de fusión en las cuales se insertaron independientemente dos de las cuatro regiones cubiertas por los péptidos (1-42 y 92-133), y se expresaron en la bacteria Escherichia coli. La inmunización con cada una de estas fusiones permitirá complementar los resultados obtenidos con los péptidos sintéticos. Dos péptidos (3-31 y 103-124) resultaron capaces de inducir la producción de anticuerpos neutralizantes contra los cuatro serotipos del virus Dengue, siendo reconocidos a su vez por múltiples sueros de pacientes con diagnóstico clínico y serológico de haber sufrido una infección por el virus Dengue, mediante la técnica de Elisa indirecto. De esta manera, la presencia de epítopes secuenciales en la proteína pre-M del virus Dengue-2 queda demostrada, así como la posible relevancia de los mismos en la respuesta inmune contra dicho flavivirus.
Priority Applications (15)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CU1997013A CU22683A1 (es) | 1997-01-15 | 1997-01-15 | Epítopes de la proteína pre-m/m del virus del dengue, péptidos sintéticos, proteínas quiméricas y sus usos |
BR9807486A BR9807486A (pt) | 1997-01-15 | 1998-01-13 | Epìtopos pre-m/m do vìrus da dengue, peptìdios, sintéticos, proteìnas quiméricas e uso das mesmas |
AU56499/98A AU743414B2 (en) | 1997-01-15 | 1998-01-13 | Epitopes of the protein pre-M/M of the dengue virus, synthetic peptides |
EP98900843A EP0966535B1 (en) | 1997-01-15 | 1998-01-13 | Epitopes of the protein pre-m/m of the dengue virus, synthetic peptides |
AT98900843T ATE284444T1 (de) | 1997-01-15 | 1998-01-13 | Epitope vom pre-m/m protein aus dem dengue-virus, synthetische peptide |
JP53348698A JP3647048B2 (ja) | 1997-01-15 | 1998-01-13 | デング熱ウイルスのプレm/mエピトープ、合成ペプチド、キメラタンパク質およびその用途 |
CA002277816A CA2277816A1 (en) | 1997-01-15 | 1998-01-13 | Epitopes of the protein pre-m/m of the dengue virus, synthetic peptides |
DE69828032T DE69828032T2 (de) | 1997-01-15 | 1998-01-13 | Epitope vom pre-m/m protein aus dem dengue-virus, synthetische peptide |
PCT/CU1998/000001 WO1998031814A1 (es) | 1997-01-15 | 1998-01-13 | Epitopes de la proteina pre-m/m del virus del dengue, peptidos sinteticos |
US09/341,833 US6383488B1 (en) | 1997-01-15 | 1998-01-13 | Pre-M/M epitopes of dengue virus, synthetic peptides, chimeric proteins and their use |
CNB988032031A CN1198933C (zh) | 1997-01-15 | 1998-01-13 | 登革病毒的前m/m蛋白的表位、合成肽 |
ES98900843T ES2235310T3 (es) | 1997-01-15 | 1998-01-13 | Epitopos de la proteina pre-m/m del virus del dengue, peptidos sinteticos. |
MYPI98000163A MY120971A (en) | 1997-01-15 | 1998-01-15 | Pre-m/m epitopes of dengue virus, synthetic peptides, chimeric proteins and its use |
NO993468A NO993468L (no) | 1997-01-15 | 1999-07-14 | Syntetiske peptid-epitoper fra proteinet fra Pre-M/M fra dengue-virus |
HK00105273A HK1025999A1 (en) | 1997-01-15 | 2000-08-22 | Epitopes of the protein pre-m/m of the dengue virus, synthetic peptides. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CU1997013A CU22683A1 (es) | 1997-01-15 | 1997-01-15 | Epítopes de la proteína pre-m/m del virus del dengue, péptidos sintéticos, proteínas quiméricas y sus usos |
Publications (1)
Publication Number | Publication Date |
---|---|
CU22683A1 true CU22683A1 (es) | 2001-07-20 |
Family
ID=5459193
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CU1997013A CU22683A1 (es) | 1997-01-15 | 1997-01-15 | Epítopes de la proteína pre-m/m del virus del dengue, péptidos sintéticos, proteínas quiméricas y sus usos |
Country Status (15)
Country | Link |
---|---|
US (1) | US6383488B1 (es) |
EP (1) | EP0966535B1 (es) |
JP (1) | JP3647048B2 (es) |
CN (1) | CN1198933C (es) |
AT (1) | ATE284444T1 (es) |
AU (1) | AU743414B2 (es) |
BR (1) | BR9807486A (es) |
CA (1) | CA2277816A1 (es) |
CU (1) | CU22683A1 (es) |
DE (1) | DE69828032T2 (es) |
ES (1) | ES2235310T3 (es) |
HK (1) | HK1025999A1 (es) |
MY (1) | MY120971A (es) |
NO (1) | NO993468L (es) |
WO (1) | WO1998031814A1 (es) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CU23245A1 (es) | 2001-07-16 | 2007-10-17 | Inst De Medicina Tropical Pedr | CADENAS QUIMéRICAS CODIFICANTES PARA PROTEINAS INDUCTORAS DE EFECTOS CONTRA VIRUS. PREPARADOS UTILIZANDO PROTEINAS QUIMéRICAS |
CA2466413C (en) * | 2001-12-04 | 2014-11-04 | Bavarian Nordic A/S | Flavivirus ns1 subunit vaccine |
EP1454988A1 (en) * | 2003-03-03 | 2004-09-08 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Infectious flavivirus pseudo-particles containing functional prM-E envelope proteins |
US7281274B2 (en) * | 2003-10-16 | 2007-10-09 | Lmp Media Llc | Electronic media distribution system |
CA2576798C (en) * | 2004-07-27 | 2014-09-09 | Gwong-Jen J. Chang | Localization and characterization of flavivirus envelope glycoprotein cross-reactive epitopes and methods for their use |
CU23578A1 (es) * | 2005-09-16 | 2010-09-30 | Ct Ingenieria Genetica Biotech | Proteína de la cápsida del virus dengue inductora de respuesta protectora y composición vacunal |
CU23630A1 (es) | 2006-10-30 | 2011-02-24 | Ct Ingenieria Genetica Biotech | Moléculas peptídicas quiméricas con propiedades antivirales contra los virus de la familia flaviviridae |
WO2009024534A2 (en) * | 2007-08-17 | 2009-02-26 | Intercell Ag | Japanese encephalitis virus (jev) and tick-borne encephalitis virus (tbev) peptides stimulating human t cell responses |
CU20080028A6 (es) * | 2008-02-29 | 2011-02-24 | Ct Ingenieria Genetica Biotech | Compuestos químicos obtenidos in silico para la preparación de composiciones farmacéuticas para atenuar o inhibir la infección por virus dengue y otros flavivirus |
CN108610416B (zh) * | 2008-10-13 | 2022-01-14 | 生物医学研究所 | 登革热病毒中和抗体及其用途 |
CN101921310B (zh) * | 2009-06-15 | 2014-03-12 | 温州医学院 | 登革病毒特异性hla-a2限制性表位肽及应用 |
WO2011133112A1 (en) * | 2010-04-20 | 2011-10-27 | National University Of Singapore | Cell penetrating peptide derived from the premembrane protein of flavivirus |
WO2012159187A2 (en) * | 2011-05-26 | 2012-11-29 | Universidade Federal Do Rio De Janeiro - Ufrj | Denv-derived peptides and methods for the inhibition of the flavivirus replication |
CU24188B1 (es) * | 2012-12-27 | 2016-07-29 | Ct De Ingeniería Genética Y Biotecnología | Composición vacunal contra el virus dengue |
SG11201510617WA (en) * | 2013-06-26 | 2016-01-28 | Univ North Carolina | Methods and compositions for dengue virus vaccines |
TWI769185B (zh) * | 2016-10-27 | 2022-07-01 | 海樂源有限公司 | 登革熱類病毒顆粒、抗登革熱病毒抗體、及含其之組合物 |
CN116082499B (zh) * | 2021-11-08 | 2023-10-27 | 东莞市朋志生物科技有限公司 | 一种抗登革ns1蛋白的抗体及其应用 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1996040933A1 (en) * | 1995-06-07 | 1996-12-19 | The Government Of The United States Of America, Represented By The Secretary Department Of Health And Human Services | Infectious dengue 2 virus pdk-53 as quadravalent vaccine |
-
1997
- 1997-01-15 CU CU1997013A patent/CU22683A1/es unknown
-
1998
- 1998-01-13 JP JP53348698A patent/JP3647048B2/ja not_active Expired - Fee Related
- 1998-01-13 BR BR9807486A patent/BR9807486A/pt not_active Application Discontinuation
- 1998-01-13 EP EP98900843A patent/EP0966535B1/en not_active Expired - Lifetime
- 1998-01-13 ES ES98900843T patent/ES2235310T3/es not_active Expired - Lifetime
- 1998-01-13 US US09/341,833 patent/US6383488B1/en not_active Expired - Fee Related
- 1998-01-13 CN CNB988032031A patent/CN1198933C/zh not_active Expired - Fee Related
- 1998-01-13 WO PCT/CU1998/000001 patent/WO1998031814A1/es active IP Right Grant
- 1998-01-13 DE DE69828032T patent/DE69828032T2/de not_active Expired - Fee Related
- 1998-01-13 AT AT98900843T patent/ATE284444T1/de not_active IP Right Cessation
- 1998-01-13 AU AU56499/98A patent/AU743414B2/en not_active Ceased
- 1998-01-13 CA CA002277816A patent/CA2277816A1/en not_active Abandoned
- 1998-01-15 MY MYPI98000163A patent/MY120971A/en unknown
-
1999
- 1999-07-14 NO NO993468A patent/NO993468L/no not_active Application Discontinuation
-
2000
- 2000-08-22 HK HK00105273A patent/HK1025999A1/xx not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
BR9807486A (pt) | 2000-03-21 |
ES2235310T3 (es) | 2005-07-01 |
NO993468D0 (no) | 1999-07-14 |
NO993468L (no) | 1999-09-14 |
WO1998031814A1 (es) | 1998-07-23 |
AU5649998A (en) | 1998-08-07 |
US6383488B1 (en) | 2002-05-07 |
JP3647048B2 (ja) | 2005-05-11 |
CN1198933C (zh) | 2005-04-27 |
CA2277816A1 (en) | 1998-07-23 |
JP2001508457A (ja) | 2001-06-26 |
CN1249781A (zh) | 2000-04-05 |
EP0966535A1 (en) | 1999-12-29 |
AU743414B2 (en) | 2002-01-24 |
HK1025999A1 (en) | 2000-12-01 |
EP0966535B1 (en) | 2004-12-08 |
DE69828032D1 (de) | 2005-01-13 |
DE69828032T2 (de) | 2005-12-01 |
ATE284444T1 (de) | 2004-12-15 |
MY120971A (en) | 2005-12-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CU22683A1 (es) | Epítopes de la proteína pre-m/m del virus del dengue, péptidos sintéticos, proteínas quiméricas y sus usos | |
ES2428869T3 (es) | Compuestos de unión, compuestos inmunógenos y peptidomiméticos | |
AU776844B2 (en) | Early detection of flaviviruses using the NS1 glycoprotein | |
BR0016510A (pt) | Aperfeçoamentos em ou relacionados as respostas imunes ao hiv | |
ES2336393T3 (es) | Complejo antigenico que comprende un inmoestimulador, cd4, y un dominio receptor de quimioquina para tratamiento de vih y desordenes inmunes. | |
AU570929B2 (en) | Broad spectrum influenza antisera | |
ES2281900T3 (es) | Peptidos sinteticos y vacunas que los contienen. | |
NO831098L (no) | Peptider med spesifiteten til munn- og klovsyke-virus-antigener | |
US11419923B2 (en) | Self-assembling synthetic proteins | |
ES2061495T3 (es) | Peptidos relacionados con el virus htlv-iii, anticuerpos para los peptidos, vacunas, inmunizacion activa y pasiva contra el virus del sida y prueba diagnostica para la deteccion serologica del virus del sida. | |
Volpina et al. | A synthetic peptide based on the NS1 non-structural protein of tick-borne encephalitis virus induces a protective immune response against fatal encephalitis in an experimental animal model | |
LOLEIT et al. | Conjugates of synthetic lymphocyte-activating lipopeptides with segments from HIV proteins induce protein-specific antibody formation | |
PT1417222E (pt) | Polipéptidos isolados baseados no epítopo neutralizante da proteína p17 de vih, úteis como vacinas, e anticorpos antip17 neutralizantes que reconhecem especificamente o referido polipéptido neutralizante | |
JPS59501866A (ja) | 腸内ウイルスに対するワクチン接種に有用なポリペプチド | |
Boucher et al. | Immune response and epitope mapping of a candidate HIV‐1 p17 vaccine HGP30 | |
WO2007010291A1 (en) | Vaccine | |
Hewer et al. | Peptide immunogens based on the envelope region of HIV-1 are recognized by HIV/AIDS patient polyclonal antibodies and induce strong humoral immune responses in mice and rabbits | |
ES2057579T3 (es) | Peptidos e1 y c de rubeola. | |
ES2535777T3 (es) | Epítopos antigénicos de la proteína reguladora del factor de virulencia en Staphylococcus aureus y sus mimótopos y uso | |
RU2723705C1 (ru) | Рекомбинантный белок для иммунизации против холеры | |
CN104151402B (zh) | 病毒性心肌炎环肽疫苗及其制备方法 | |
BR102017020980A2 (pt) | Sequência peptídica mimética de aflatoxina aplicada como imunógeno | |
JP4568842B2 (ja) | 熱帯熱マラリア原虫のエノラーゼ蛋白質の部分ペプチドの製造方法 | |
Garcia et al. | Analysis of the immune response induced by a scorpion venom sub-fraction, a pure peptide and a recombinant peptide, against toxin Cn2 of Centruroides noxius Hoffmann | |
JPS60501360A (ja) | 大腸菌由来の熱安定性エンテロトキシンポリペプチドの合成品及びその多量体 |