CS207473B2 - Method of decreasing the contents of lipids and nucleus acids in the microbial cell substance - Google Patents
Method of decreasing the contents of lipids and nucleus acids in the microbial cell substance Download PDFInfo
- Publication number
- CS207473B2 CS207473B2 CS764982A CS498277A CS207473B2 CS 207473 B2 CS207473 B2 CS 207473B2 CS 764982 A CS764982 A CS 764982A CS 498277 A CS498277 A CS 498277A CS 207473 B2 CS207473 B2 CS 207473B2
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- cell mass
- weight
- solvent
- ammonia
- methanol
- Prior art date
Links
- 150000002632 lipids Chemical class 0.000 title claims abstract description 22
- 230000000813 microbial effect Effects 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 title claims description 32
- 230000003247 decreasing effect Effects 0.000 title abstract 2
- 239000002253 acid Substances 0.000 title description 2
- 239000000126 substance Substances 0.000 title description 2
- 150000007513 acids Chemical class 0.000 title 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims abstract description 62
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000000203 mixture Substances 0.000 claims abstract description 27
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 25
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 25
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 25
- 229910021529 ammonia Inorganic materials 0.000 claims abstract description 22
- 238000000605 extraction Methods 0.000 claims abstract description 13
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims abstract description 11
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000003960 organic solvent Substances 0.000 claims abstract description 10
- 239000008346 aqueous phase Substances 0.000 claims abstract description 5
- 239000000908 ammonium hydroxide Substances 0.000 claims abstract description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical group OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 78
- 239000002904 solvent Substances 0.000 claims description 31
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 25
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 4
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 4
- XNWFRZJHXBZDAG-UHFFFAOYSA-N 2-METHOXYETHANOL Chemical compound COCCO XNWFRZJHXBZDAG-UHFFFAOYSA-N 0.000 claims description 3
- 239000001257 hydrogen Substances 0.000 claims description 3
- 229910052739 hydrogen Inorganic materials 0.000 claims description 3
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 claims description 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 2
- 230000029087 digestion Effects 0.000 claims 2
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims 1
- 150000002431 hydrogen Chemical class 0.000 claims 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 40
- 239000000047 product Substances 0.000 description 12
- 239000000463 material Substances 0.000 description 11
- 239000000243 solution Substances 0.000 description 10
- 241000589344 Methylomonas Species 0.000 description 9
- 239000007858 starting material Substances 0.000 description 9
- 235000011114 ammonium hydroxide Nutrition 0.000 description 8
- 239000000725 suspension Substances 0.000 description 8
- 239000007787 solid Substances 0.000 description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 6
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonium chloride Substances [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 5
- 239000003925 fat Substances 0.000 description 5
- 238000001914 filtration Methods 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 239000013049 sediment Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 241000235015 Yarrowia lipolytica Species 0.000 description 4
- 235000013861 fat-free Nutrition 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 210000005253 yeast cell Anatomy 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- CBHOOMGKXCMKIR-UHFFFAOYSA-N azane;methanol Chemical compound N.OC CBHOOMGKXCMKIR-UHFFFAOYSA-N 0.000 description 3
- HXCHRJMJMALFHP-UHFFFAOYSA-N azanium;ethanol;hydroxide Chemical compound N.O.CCO HXCHRJMJMALFHP-UHFFFAOYSA-N 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 229930195733 hydrocarbon Natural products 0.000 description 3
- 150000002430 hydrocarbons Chemical class 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 230000009965 odorless effect Effects 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000012065 filter cake Substances 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000021588 free fatty acids Nutrition 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 229930000044 secondary metabolite Natural products 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 238000005292 vacuum distillation Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 241000235646 Cyberlindnera jadinii Species 0.000 description 1
- 108010058643 Fungal Proteins Proteins 0.000 description 1
- 210000000712 G cell Anatomy 0.000 description 1
- 201000005569 Gout Diseases 0.000 description 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241000589341 Methylomonas clara Species 0.000 description 1
- 241000228150 Penicillium chrysogenum Species 0.000 description 1
- 208000009911 Urinary Calculi Diseases 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000003916 acid precipitation Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 150000008280 chlorinated hydrocarbons Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000000779 depleting effect Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- -1 ethanol or methanol Chemical compound 0.000 description 1
- 150000002170 ethers Chemical class 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 125000005456 glyceride group Chemical group 0.000 description 1
- 150000002334 glycols Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000011110 re-filtration Methods 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 229930195734 saturated hydrocarbon Natural products 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 208000008281 urolithiasis Diseases 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/08—Reducing the nucleic acid content
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Extraction Or Liquid Replacement (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2633666A DE2633666C3 (de) | 1976-07-27 | 1976-07-27 | Verminderung des Lipid- und Nucleinsäure-Gehaltes in mikrobiellen Zellmassen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CS207473B2 true CS207473B2 (en) | 1981-07-31 |
Family
ID=5984032
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS764982A CS207473B2 (en) | 1976-07-27 | 1977-07-27 | Method of decreasing the contents of lipids and nucleus acids in the microbial cell substance |
Country Status (28)
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2748885A1 (de) * | 1977-11-02 | 1979-05-03 | Hoechst Ag | Verfahren zur verbesserung der eigenschaften von schroten oder mehlen aus oelsaaten |
| DE2907065A1 (de) | 1979-02-23 | 1980-09-04 | Hoechst Ag | Verfahren zum fetten von leder und pelzfellen |
| CA1120314A (en) * | 1979-08-22 | 1982-03-23 | John A. Ridgway | Treatment of proteinaceous materials with anhydrous ammonia gas |
| DE3143947A1 (de) * | 1981-11-05 | 1983-05-11 | Hoechst Ag, 6230 Frankfurt | "funktionelle eiweisshydrolysate, verfahren zu ihrer herstellung und diese eiweisshydrolysate enthaltende nahrungsmittel" |
| DE3228500A1 (de) * | 1982-07-30 | 1984-02-02 | Basf Ag, 6700 Ludwigshafen | Verfahren zur reinigung von aldehyde, acetale und/oder ungesaettigte verbindungen enthaltenden carbonsaeureestern |
| WO1996038535A1 (fr) * | 1995-05-29 | 1996-12-05 | Nauchno-Issledovatelsky Institut Vychislitelnykh Komplexov | Procede d'obtention d'une biomasse de micro-organismes ayant un faible contenu en acides nucleiques |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3615654A (en) * | 1968-05-17 | 1971-10-26 | Cpc International Inc | Method of treating microbial cells |
| IT954172B (it) * | 1970-07-24 | 1973-08-30 | Standard Oil Co | Procedimento per estrarre materiale proteico da organismi microbici unicellulari e prodotto ottenuto |
| SU419551A1 (ru) * | 1972-01-18 | 1974-03-15 | В. Ф. Фоменков Институт биологической физики СССР | Тепловой расходомер |
| GB1400691A (en) * | 1973-02-08 | 1975-07-23 | British Petroleum Co | Process for the production of proteinaceous material |
| GB1408845A (en) * | 1973-02-13 | 1975-10-08 | Ranks Hovis Mcdougall Ltd | Production of edible protein containing substances |
| JPS5411379B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1973-02-21 | 1979-05-15 |
-
1976
- 1976-07-27 DE DE2633666A patent/DE2633666C3/de not_active Expired
-
1977
- 1977-07-20 ES ES460877A patent/ES460877A1/es not_active Expired
- 1977-07-22 CH CH913977A patent/CH635615A5/de not_active IP Right Cessation
- 1977-07-22 NL NL7708171A patent/NL7708171A/xx not_active Application Discontinuation
- 1977-07-22 BG BG036986A patent/BG37997A3/xx unknown
- 1977-07-25 SE SE7708539A patent/SE432609B/xx not_active IP Right Cessation
- 1977-07-25 IL IL52594A patent/IL52594A/xx unknown
- 1977-07-25 EG EG439/77A patent/EG12626A/xx active
- 1977-07-25 GR GR54033A patent/GR64056B/el unknown
- 1977-07-25 RO RO7791163A patent/RO71982A/ro unknown
- 1977-07-25 DD DD7700200262A patent/DD131072A5/xx unknown
- 1977-07-25 FI FI772272A patent/FI57443C/fi not_active IP Right Cessation
- 1977-07-25 IT IT26084/77A patent/IT1082226B/it active
- 1977-07-26 AT AT544077A patent/AT357502B/de not_active IP Right Cessation
- 1977-07-26 ZA ZA00774517A patent/ZA774517B/xx unknown
- 1977-07-26 NO NO772658A patent/NO147034C/no unknown
- 1977-07-26 AU AU27319/77A patent/AU512670B2/en not_active Expired
- 1977-07-26 HU HU77HO2006A patent/HU176802B/hu unknown
- 1977-07-26 CA CA283,487A patent/CA1101724A/en not_active Expired
- 1977-07-26 SU SU772504953A patent/SU791257A3/ru active
- 1977-07-26 DK DK337677A patent/DK146511C/da not_active IP Right Cessation
- 1977-07-26 PT PT66849A patent/PT66849B/pt unknown
- 1977-07-26 PL PL1977199855A patent/PL107949B1/pl unknown
- 1977-07-27 CS CS764982A patent/CS207473B2/cs unknown
- 1977-07-27 FR FR7723082A patent/FR2359896A1/fr active Granted
- 1977-07-27 BE BE179698A patent/BE857229A/xx not_active IP Right Cessation
- 1977-07-27 JP JP8934977A patent/JPS5315489A/ja active Granted
- 1977-07-27 GB GB31537/77A patent/GB1584194A/en not_active Expired
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