CO7461131A2 - Antígenos recombinantes del porcine circovirus 2 (pcv-2) para formulaciones de vacuna, kit de diagnóstico y uso - Google Patents

Antígenos recombinantes del porcine circovirus 2 (pcv-2) para formulaciones de vacuna, kit de diagnóstico y uso

Info

Publication number
CO7461131A2
CO7461131A2 CO15164030A CO15164030A CO7461131A2 CO 7461131 A2 CO7461131 A2 CO 7461131A2 CO 15164030 A CO15164030 A CO 15164030A CO 15164030 A CO15164030 A CO 15164030A CO 7461131 A2 CO7461131 A2 CO 7461131A2
Authority
CO
Colombia
Prior art keywords
pcv
vaccine formulations
diagnostic kit
porcine circovirus
recombinant antigens
Prior art date
Application number
CO15164030A
Other languages
English (en)
Inventor
De Souza Luiz Fernando Lino
Silva Leite Roberta Amaziles
Morais Monteiro Antonio De
Cruz Souza Amanda Martins Da
Gonzaga Natalia Fialho
Teixeira Jackson De Andrade
Zilch Tiago Jaquel
De Almeida Marcia Rogeria
Junio Abelardo Silva
Rangel Fietto Juliana Lopes
Bressan Gustavo Costa
Salgado Rafael Locatelli
Onofre Thiago Souza
Fausto Mariana Costa
Vidigal Pedro Marcus Pereira
Kalks Sthefany Patareli
Crispim Josicelli Souza
Original Assignee
Fundacao De Amparo A Pesquisa Do Estado De Minas Gerais Fapeming
Univ Fed Vicosa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fundacao De Amparo A Pesquisa Do Estado De Minas Gerais Fapeming, Univ Fed Vicosa filed Critical Fundacao De Amparo A Pesquisa Do Estado De Minas Gerais Fapeming
Publication of CO7461131A2 publication Critical patent/CO7461131A2/es

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5258—Virus-like particles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55505—Inorganic adjuvants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/10011—Circoviridae
    • C12N2750/10023—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/10011—Circoviridae
    • C12N2750/10034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/015—Parvoviridae, e.g. feline panleukopenia virus, human Parvovirus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00—Immunoassays for the detection of microorganisms
    • G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)

Abstract

La presente invención se refiere a la obtención de antígeno recombinante de la cápside viral de Porcine circovirus2 (PCV-2) y sus modificaciones, por expresión en sistema procarionte, purificación en forma monomérica, la recuperación de partícula similar a virus (VLP) y su uso en formulaciones de vacuna, kits de diagnóstico y un sistema de cuantificación de lotes de vacuna del antígeno del PVC-2 por un ELISA de captura. Antígenos y formulaciones de vacuna pueden ser usados en la inmunización de animales en programas de control de las enfermedades asociadas con PCV-2 en los sistemas de producción porcinos convencionales y representan alternativas a las vacunas disponibles en el mercado. El kit ELISA se puede utilizar para pruebas de calidad en las vacunas comerciales y/o experimentales contra PCV-2.
CO15164030A 2013-01-25 2015-07-15 Antígenos recombinantes del porcine circovirus 2 (pcv-2) para formulaciones de vacuna, kit de diagnóstico y uso CO7461131A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BR102013001893-7A BR102013001893B1 (pt) 2013-01-25 2013-01-25 Antígenos recombinantes do porcine circovirus 2 (pcv-2) para formulações vacinais, kit de diagnóstico e uso

Publications (1)

Publication Number Publication Date
CO7461131A2 true CO7461131A2 (es) 2015-11-30

Family

ID=51226766

Family Applications (1)

Application Number Title Priority Date Filing Date
CO15164030A CO7461131A2 (es) 2013-01-25 2015-07-15 Antígenos recombinantes del porcine circovirus 2 (pcv-2) para formulaciones de vacuna, kit de diagnóstico y uso

Country Status (11)

Country Link
US (2) US9717785B2 (es)
EP (1) EP2949341B1 (es)
CN (1) CN105263515A (es)
AR (1) AR094578A1 (es)
BR (1) BR102013001893B1 (es)
CO (1) CO7461131A2 (es)
ES (1) ES2792510T3 (es)
MX (1) MX362820B (es)
RU (1) RU2687001C2 (es)
UY (1) UY35272A (es)
WO (1) WO2014113855A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106478783B (zh) * 2015-08-24 2021-11-02 兆丰华生物科技(南京)有限公司北京生物医药科技中心 一种猪圆环病毒ii型基因工程亚单位疫苗及其应用
CN105541976B (zh) * 2015-12-25 2019-07-09 华南农业大学 猪圆环病毒2型Cap基因修饰改造重组抗原及其应用
CN109425738B (zh) * 2017-09-01 2022-01-28 洛阳普泰生物技术有限公司 猪圆环病毒3型抗体检测试剂盒及其应用
CN109187774B (zh) * 2018-07-03 2021-09-28 复旦大学 一种猪圆环病毒2型病毒样颗粒的定量检测方法
CN110981945B (zh) * 2019-12-08 2021-10-19 中牧实业股份有限公司 一种猪圆环病毒2型重组Cap蛋白的表达制备及应用
CN111233983A (zh) * 2020-02-26 2020-06-05 郭广君 猪圆环病毒cap蛋白一步法大规模纯化及内毒素去除工艺
CN114354526A (zh) * 2021-12-28 2022-04-15 国药集团动物保健股份有限公司 一种猪圆环病毒3型纯品蛋白的定量检测方法
CN114805501A (zh) * 2022-03-24 2022-07-29 中牧实业股份有限公司 一种猪圆环病毒2型抗原组合物及其在抗原定量方法中的应用

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FR2769322B1 (fr) * 1997-10-03 2002-03-08 Merial Sas Nouveaux circovirus porcins, vaccins et reactifs de diagnostic
AU770002B2 (en) 1998-03-13 2004-02-12 University Of Georgia Research Foundation, Inc., The Vaccines against circovirus infections
UA84024C2 (ru) 2003-07-24 2008-09-10 Мериал Лимитед Новые вакцинные композиции, которые содержат эмульсию типа "масло в воде"
US7700285B1 (en) 2005-12-29 2010-04-20 Boehringer Ingelheim Vetmedica, Inc. PCV2 immunogenic compositions and methods of producing such compositions
UA95458C2 (ru) 2005-09-09 2011-08-10 Интервет Интернешонал Б.В. Вакцина против pcv-2 у mda-положительных поросят
EP1968630B1 (en) 2005-12-29 2018-01-24 Boehringer Ingelheim Vetmedica, Inc. Multivalent pcv2 immunogenic compositions
NZ586238A (en) * 2007-12-21 2012-10-26 Wyeth Llc Methods and compositions for immunizing pigs against porcine circovirus
WO2010061000A1 (en) * 2008-11-28 2010-06-03 Ceva Sante Animale Novel porcine circovirus type 2b isolate and uses thereof
CN102711816A (zh) * 2009-10-22 2012-10-03 莱比锡大学 患有牛新生儿全血细胞减少症的小牛中的圆环病毒的检测
CN102174086B (zh) 2011-03-07 2014-04-02 南京农业大学 一种猪圆环病毒2型重组Cap蛋白和亚单位疫苗
CN102911947A (zh) * 2011-08-01 2013-02-06 普莱柯生物工程股份有限公司 猪圆环病毒2型Cap蛋白及其制备方法
CN102839195A (zh) * 2012-07-18 2012-12-26 华南农业大学 利用pFast Bac Dual杆状病毒表达PCV 2 Cap蛋白的方法

Also Published As

Publication number Publication date
US20160000897A1 (en) 2016-01-07
UY35272A (es) 2014-08-29
MX2015009507A (es) 2016-04-07
MX362820B (es) 2019-02-18
EP2949341B1 (en) 2020-04-01
EP2949341A1 (en) 2015-12-02
RU2015135619A (ru) 2017-03-10
BR102013001893A8 (pt) 2017-12-05
RU2687001C2 (ru) 2019-05-06
WO2014113855A1 (pt) 2014-07-31
BR102013001893A2 (pt) 2014-09-09
EP2949341A4 (en) 2016-07-27
US9717785B2 (en) 2017-08-01
CN105263515A (zh) 2016-01-20
US20160015799A1 (en) 2016-01-21
AR094578A1 (es) 2015-08-12
BR102013001893B1 (pt) 2022-01-25
ES2792510T3 (es) 2020-11-11

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