HRP20171697T1 - Varijanta hiv gp-120 - Google Patents

Varijanta hiv gp-120 Download PDF

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Publication number
HRP20171697T1
HRP20171697T1 HRP20171697TT HRP20171697T HRP20171697T1 HR P20171697 T1 HRP20171697 T1 HR P20171697T1 HR P20171697T T HRP20171697T T HR P20171697TT HR P20171697 T HRP20171697 T HR P20171697T HR P20171697 T1 HRP20171697 T1 HR P20171697T1
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Croatia
Prior art keywords
polypeptide
fact
polynucleotide
sample
neutralizing antibodies
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HRP20171697TT
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English (en)
Inventor
María Eloísa Yuste Herranz
Víctor SÁNCHEZ MERINO
Carolina Ferreira
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Laboratorios Del Dr. Esteve, S.A.
Fundació Privada Institut De Recerca De La Sida-Caixa
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Publication of HRP20171697T1 publication Critical patent/HRP20171697T1/hr

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08RNA viruses
    • C07K14/15Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus human T-cell leukaemia-lymphoma virus
    • C07K14/155Lentiviridae, e.g. human immunodeficiency virus [HIV], visna-maedi virus or equine infectious anaemia virus
    • C07K14/16HIV-1 ; HIV-2
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/12Viral antigens
    • A61K39/21Retroviridae, e.g. equine infectious anemia virus
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • A61P31/14Antivirals for RNA viruses
    • A61P31/18Antivirals for RNA viruses for HIV
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1036Retroviridae, e.g. leukemia viruses
    • C07K16/1045Lentiviridae, e.g. HIV, FIV, SIV
    • C07K16/1063Lentiviridae, e.g. HIV, FIV, SIV env, e.g. gp41, gp110/120, gp160, V3, PND, CD4 binding site
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1037Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983Viruses
    • G01N33/56988HIV or HTLV
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854Immunoglobulins
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/70Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76Antagonist effect on antigen, e.g. neutralization or inhibition of binding
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/16011Human Immunodeficiency Virus, HIV
    • C12N2740/16041Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/16011Human Immunodeficiency Virus, HIV
    • C12N2740/16111Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • CCHEMISTRY; METALLURGY
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    • C12N2799/00Uses of viruses
    • C12N2799/02Uses of viruses as vector
    • C12N2799/021Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08RNA viruses
    • G01N2333/15Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16HIV-1, HIV-2
    • G01N2333/162HIV-1, HIV-2 env, e.g. gp160, gp110/120, gp41, V3, peptid T, DC4-Binding site
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00Immunoassays for the detection of microorganisms
    • G01N2469/20Detection of antibodies in sample from host which are directed against antigens from microorganisms

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
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  • Biochemistry (AREA)
  • Hematology (AREA)
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  • Medicinal Chemistry (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
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  • AIDS & HIV (AREA)
  • Gastroenterology & Hepatology (AREA)
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  • Tropical Medicine & Parasitology (AREA)
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Claims (13)

1. Polipeptid koji može izazvati stvaranje neutralizirajućih protutijela protiv virusa koji sadrži varijantu HIV-1 gp120 ili njegov imunogeni fragment, naznačen time što polipeptid ili njegov imunogeni fragment sadrži slijed sa SEQ ID NO:4.
2. Protutijelo, naznačeno time što se specifično veže na polipeptid u skladu s patentnim zahtjevom 1.
3. Polinukleotid, naznačen time što kodira polipeptid u skladu s patentnim zahtjevom 1.
4. Polinukleotid u skladu s patentnim zahtjevom 3, naznačen time što ima slijed sa SEQ ID NO:3.
5. Ekspresijski vektor, naznačen time što sadrži polinukleotid u skladu s patentnim zahtjevom 4.
6. Stanica domaćin, naznačena time što sadrži polipeptid u skladu s patentnim zahtjevom 1, polinukleotid u skladu s patentnim zahtjevom 3 ili ekspresijski vektor u skladu s patentnim zahtjevom 5.
7. Imunogeni pripravak ili cjepivo, naznačeni time što sadrže polipeptid u skladu s patentnim zahtjevom 1, polinukleotid u skladu s patentnim zahtjevom 3 ili ekspresijski vektor u skladu s patentnim zahtjevom 5.
8. Polipeptid u skladu s patentnim zahtjevom 1, polinukleotid u skladu s patentnim zahtjevom 3, ekspresijski vektor u skladu s patentnim zahtjevom 5 ili imunogeni pripravak ili cjepivo u skladu s patentnim zahtjevom 7, naznačeni time što su namijenjeni upotrebi u medicini.
9. Polipeptid u skladu s patentnim zahtjevom 1, polinukleotid u skladu s patentnim zahtjevom 3, ekspresijski vektor u skladu s patentnim zahtjevom 5 ili imunogeni pripravak ili cjepivo u skladu s patentnim zahtjevom 7, naznačeni time što su namijenjeni upotrebi u sprječavanju bolesti uzrokovane infekcijom HIV-om.
10. Postupak detektiranja u uzorku neutralizirajućih protutijela specifičnih za dani patogeni, naznačen time što obuhvaća: (i) stavljanje u kontakt navedenog uzorka s polipeptidom u skladu s patentnim zahtjevom 1 i (ii) detektiranje nastajanja imunosnog kompleksa između navedenog polipeptida i navedenih neutralizirajućih protutijela.
11. Postupak detektiranja in vitro neutralizirajućeg protutijela kao odgovora na virusnu infekciju kod subjekta, naznačen time što obuhvaća detektiranje u uzorku iz navedenog subjekta prisutnosti neutralizirajućih protutijela postupkom u skladu s patentnim zahtjevom 10, gdje prisutnost neutralizirajućih protutijela u navedenom uzorku za razliku od kontrolnog subjekta ukazuju na neutralizirajuće protutijelo kao odgovor na virusnu infekciju kod navedenog subjekta.
12. Postupak u skladu s patentnim zahtjevima 10 ili 11, naznačen time što je virus HIV.
13. Postupak u skladu s patentnim zahtjevom 12, naznačen time što je uzorak dobiven iz pacijenta zaraženog virusom HIV-1 ili, što je uzorak dobiven iz primatelja cjepiva protiv AIDS-a.
HRP20171697TT 2011-02-25 2017-11-06 Varijanta hiv gp-120 HRP20171697T1 (hr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161446595P 2011-02-25 2011-02-25
EP11382051A EP2492279A1 (en) 2011-02-25 2011-02-25 Rapid immunogen selection method using lentiviral display
PCT/EP2012/053185 WO2012113921A1 (en) 2011-02-25 2012-02-24 Rapid selection method for hiv gp-120 variants
EP12705689.3A EP2678351B1 (en) 2011-02-25 2012-02-24 Hiv gp-120 variant

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HRP20171697T1 true HRP20171697T1 (hr) 2018-01-26

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HRP20171697TT HRP20171697T1 (hr) 2011-02-25 2017-11-06 Varijanta hiv gp-120

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US (1) US9605030B2 (hr)
EP (2) EP2492279A1 (hr)
JP (1) JP6140614B2 (hr)
KR (1) KR20140025356A (hr)
CN (1) CN103459410B (hr)
AU (1) AU2012219418C1 (hr)
CA (1) CA2827967A1 (hr)
CY (1) CY1119638T1 (hr)
DK (1) DK2678351T3 (hr)
ES (1) ES2651124T3 (hr)
HR (1) HRP20171697T1 (hr)
HU (1) HUE035432T2 (hr)
IL (1) IL228094A0 (hr)
LT (1) LT2678351T (hr)
ME (1) ME03045B (hr)
MX (1) MX348459B (hr)
PL (1) PL2678351T3 (hr)
PT (1) PT2678351T (hr)
RS (1) RS56707B1 (hr)
RU (1) RU2603732C2 (hr)
SI (1) SI2678351T1 (hr)
WO (1) WO2012113921A1 (hr)

Families Citing this family (5)

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Publication number Priority date Publication date Assignee Title
EP2492279A1 (en) 2011-02-25 2012-08-29 Laboratorios Del. Dr. Esteve, S.A. Rapid immunogen selection method using lentiviral display
EP2698377A1 (en) * 2012-08-17 2014-02-19 Laboratorios Del. Dr. Esteve, S.A. Enhanced rapid immunogen selection method for HIV gp120 variants
CN104697830B (zh) * 2015-02-10 2018-06-15 深圳市新产业生物医学工程股份有限公司 用于hiv检测的酸性处理剂、样本预处理方法、试剂盒及检测方法
CN107988428B (zh) * 2017-11-10 2021-04-06 杭州众测生物科技有限公司 虾虹彩病毒(siv)的raa恒温荧光检测方法及试剂
JP2019153618A (ja) * 2018-02-28 2019-09-12 株式会社ジャパンディスプレイ 光センサ装置

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MX2013009757A (es) 2013-12-02
KR20140025356A (ko) 2014-03-04
RU2013143295A (ru) 2015-03-27
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US20140037667A1 (en) 2014-02-06
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