HRP20171697T1 - Varijanta hiv gp-120 - Google Patents

Varijanta hiv gp-120 Download PDF

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Publication number
HRP20171697T1
HRP20171697T1 HRP20171697TT HRP20171697T HRP20171697T1 HR P20171697 T1 HRP20171697 T1 HR P20171697T1 HR P20171697T T HRP20171697T T HR P20171697TT HR P20171697 T HRP20171697 T HR P20171697T HR P20171697 T1 HRP20171697 T1 HR P20171697T1
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HR
Croatia
Prior art keywords
polypeptide
fact
polynucleotide
sample
neutralizing antibodies
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HRP20171697TT
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English (en)
Inventor
María Eloísa Yuste Herranz
Víctor SÁNCHEZ MERINO
Carolina Ferreira
Original Assignee
Laboratorios Del Dr. Esteve, S.A.
Fundació Privada Institut De Recerca De La Sida-Caixa
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Publication of HRP20171697T1 publication Critical patent/HRP20171697T1/hr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus human T-cell leukaemia-lymphoma virus
    • C07K14/155—Lentiviridae, e.g. human immunodeficiency virus [HIV], visna-maedi virus or equine infectious anaemia virus
    • C07K14/16—HIV-1 ; HIV-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/21—Retroviridae, e.g. equine infectious anemia virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1036—Retroviridae, e.g. leukemia viruses
    • C07K16/1045—Lentiviridae, e.g. HIV, FIV, SIV
    • C07K16/1063—Lentiviridae, e.g. HIV, FIV, SIV env, e.g. gp41, gp110/120, gp160, V3, PND, CD4 binding site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2
    • G01N2333/162—HIV-1, HIV-2 env, e.g. gp160, gp110/120, gp41, V3, peptid T, DC4-Binding site
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00—Immunoassays for the detection of microorganisms
    • G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • AIDS & HIV (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)

Claims (13)

1. Polipeptid koji može izazvati stvaranje neutralizirajućih protutijela protiv virusa koji sadrži varijantu HIV-1 gp120 ili njegov imunogeni fragment, naznačen time što polipeptid ili njegov imunogeni fragment sadrži slijed sa SEQ ID NO:4.
2. Protutijelo, naznačeno time što se specifično veže na polipeptid u skladu s patentnim zahtjevom 1.
3. Polinukleotid, naznačen time što kodira polipeptid u skladu s patentnim zahtjevom 1.
4. Polinukleotid u skladu s patentnim zahtjevom 3, naznačen time što ima slijed sa SEQ ID NO:3.
5. Ekspresijski vektor, naznačen time što sadrži polinukleotid u skladu s patentnim zahtjevom 4.
6. Stanica domaćin, naznačena time što sadrži polipeptid u skladu s patentnim zahtjevom 1, polinukleotid u skladu s patentnim zahtjevom 3 ili ekspresijski vektor u skladu s patentnim zahtjevom 5.
7. Imunogeni pripravak ili cjepivo, naznačeni time što sadrže polipeptid u skladu s patentnim zahtjevom 1, polinukleotid u skladu s patentnim zahtjevom 3 ili ekspresijski vektor u skladu s patentnim zahtjevom 5.
8. Polipeptid u skladu s patentnim zahtjevom 1, polinukleotid u skladu s patentnim zahtjevom 3, ekspresijski vektor u skladu s patentnim zahtjevom 5 ili imunogeni pripravak ili cjepivo u skladu s patentnim zahtjevom 7, naznačeni time što su namijenjeni upotrebi u medicini.
9. Polipeptid u skladu s patentnim zahtjevom 1, polinukleotid u skladu s patentnim zahtjevom 3, ekspresijski vektor u skladu s patentnim zahtjevom 5 ili imunogeni pripravak ili cjepivo u skladu s patentnim zahtjevom 7, naznačeni time što su namijenjeni upotrebi u sprječavanju bolesti uzrokovane infekcijom HIV-om.
10. Postupak detektiranja u uzorku neutralizirajućih protutijela specifičnih za dani patogeni, naznačen time što obuhvaća: (i) stavljanje u kontakt navedenog uzorka s polipeptidom u skladu s patentnim zahtjevom 1 i (ii) detektiranje nastajanja imunosnog kompleksa između navedenog polipeptida i navedenih neutralizirajućih protutijela.
11. Postupak detektiranja in vitro neutralizirajućeg protutijela kao odgovora na virusnu infekciju kod subjekta, naznačen time što obuhvaća detektiranje u uzorku iz navedenog subjekta prisutnosti neutralizirajućih protutijela postupkom u skladu s patentnim zahtjevom 10, gdje prisutnost neutralizirajućih protutijela u navedenom uzorku za razliku od kontrolnog subjekta ukazuju na neutralizirajuće protutijelo kao odgovor na virusnu infekciju kod navedenog subjekta.
12. Postupak u skladu s patentnim zahtjevima 10 ili 11, naznačen time što je virus HIV.
13. Postupak u skladu s patentnim zahtjevom 12, naznačen time što je uzorak dobiven iz pacijenta zaraženog virusom HIV-1 ili, što je uzorak dobiven iz primatelja cjepiva protiv AIDS-a.
HRP20171697TT 2011-02-25 2017-11-06 Varijanta hiv gp-120 HRP20171697T1 (hr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161446595P 2011-02-25 2011-02-25
EP11382051A EP2492279A1 (en) 2011-02-25 2011-02-25 Rapid immunogen selection method using lentiviral display
EP12705689.3A EP2678351B1 (en) 2011-02-25 2012-02-24 Hiv gp-120 variant
PCT/EP2012/053185 WO2012113921A1 (en) 2011-02-25 2012-02-24 Rapid selection method for hiv gp-120 variants

Publications (1)

Publication Number Publication Date
HRP20171697T1 true HRP20171697T1 (hr) 2018-01-26

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HRP20171697TT HRP20171697T1 (hr) 2011-02-25 2017-11-06 Varijanta hiv gp-120

Country Status (22)

Country Link
US (1) US9605030B2 (hr)
EP (2) EP2492279A1 (hr)
JP (1) JP6140614B2 (hr)
KR (1) KR20140025356A (hr)
CN (1) CN103459410B (hr)
AU (1) AU2012219418C1 (hr)
CA (1) CA2827967A1 (hr)
CY (1) CY1119638T1 (hr)
DK (1) DK2678351T3 (hr)
ES (1) ES2651124T3 (hr)
HR (1) HRP20171697T1 (hr)
HU (1) HUE035432T2 (hr)
IL (1) IL228094A0 (hr)
LT (1) LT2678351T (hr)
ME (1) ME03045B (hr)
MX (1) MX348459B (hr)
PL (1) PL2678351T3 (hr)
PT (1) PT2678351T (hr)
RS (1) RS56707B1 (hr)
RU (1) RU2603732C2 (hr)
SI (1) SI2678351T1 (hr)
WO (1) WO2012113921A1 (hr)

Families Citing this family (5)

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Publication number Priority date Publication date Assignee Title
EP2492279A1 (en) 2011-02-25 2012-08-29 Laboratorios Del. Dr. Esteve, S.A. Rapid immunogen selection method using lentiviral display
EP2698377A1 (en) * 2012-08-17 2014-02-19 Laboratorios Del. Dr. Esteve, S.A. Enhanced rapid immunogen selection method for HIV gp120 variants
CN104697830B (zh) * 2015-02-10 2018-06-15 深圳市新产业生物医学工程股份有限公司 用于hiv检测的酸性处理剂、样本预处理方法、试剂盒及检测方法
CN107988428B (zh) * 2017-11-10 2021-04-06 杭州众测生物科技有限公司 虾虹彩病毒(siv)的raa恒温荧光检测方法及试剂
JP2019153618A (ja) * 2018-02-28 2019-09-12 株式会社ジャパンディスプレイ 光センサ装置

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US20140037667A1 (en) 2014-02-06
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