CN1967252B - 直接胆红素测定试剂盒 - Google Patents
直接胆红素测定试剂盒 Download PDFInfo
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- CN1967252B CN1967252B CN2006101544817A CN200610154481A CN1967252B CN 1967252 B CN1967252 B CN 1967252B CN 2006101544817 A CN2006101544817 A CN 2006101544817A CN 200610154481 A CN200610154481 A CN 200610154481A CN 1967252 B CN1967252 B CN 1967252B
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- reagent
- test kit
- kit
- bilirubin direct
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- 238000008789 Direct Bilirubin Methods 0.000 title abstract 2
- 238000012360 testing method Methods 0.000 claims abstract description 70
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 54
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 claims description 100
- 239000007788 liquid Substances 0.000 claims 1
- 239000002904 solvent Substances 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 abstract description 22
- 235000010288 sodium nitrite Nutrition 0.000 abstract description 11
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 abstract description 6
- 230000035945 sensitivity Effects 0.000 abstract description 5
- 239000007853 buffer solution Substances 0.000 abstract description 3
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 abstract description 2
- BDOYKFSQFYNPKF-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;sodium Chemical compound [Na].[Na].OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O BDOYKFSQFYNPKF-UHFFFAOYSA-N 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 2
- PPKXEPBICJTCRU-XMZRARIVSA-N (R,R)-tramadol hydrochloride Chemical compound Cl.COC1=CC=CC([C@]2(O)[C@H](CCCC2)CN(C)C)=C1 PPKXEPBICJTCRU-XMZRARIVSA-N 0.000 abstract 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Natural products NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 abstract 1
- 229910000397 disodium phosphate Inorganic materials 0.000 abstract 1
- 235000019800 disodium phosphate Nutrition 0.000 abstract 1
- RBLWMQWAHONKNC-UHFFFAOYSA-N hydroxyazanium Chemical compound O[NH3+] RBLWMQWAHONKNC-UHFFFAOYSA-N 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 229960004906 thiomersal Drugs 0.000 abstract 1
- 229960003107 tramadol hydrochloride Drugs 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 18
- 238000006243 chemical reaction Methods 0.000 description 15
- 238000005259 measurement Methods 0.000 description 12
- 238000000034 method Methods 0.000 description 12
- 239000000047 product Substances 0.000 description 10
- 238000003908 quality control method Methods 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- BPYKTIZUTYGOLE-UHFFFAOYSA-N billirubin-IXalpha Natural products N1C(=O)C(C)=C(C=C)C1=CC1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(C=C3C(=C(C=C)C(=O)N3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-UHFFFAOYSA-N 0.000 description 4
- 230000000536 complexating effect Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 3
- 125000000664 diazo group Chemical group [N-]=[N+]=[*] 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- RCNSAJSGRJSBKK-NSQVQWHSSA-N Biliverdin IX Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(\C=C/2C(=C(C)C(=C/C=3C(=C(C=C)C(=O)N=3)C)/N\2)CCC(O)=O)N1 RCNSAJSGRJSBKK-NSQVQWHSSA-N 0.000 description 2
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 description 2
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 2
- WTDHULULXKLSOZ-UHFFFAOYSA-N Hydroxylamine hydrochloride Chemical compound Cl.ON WTDHULULXKLSOZ-UHFFFAOYSA-N 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 238000006701 autoxidation reaction Methods 0.000 description 2
- 239000012482 calibration solution Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 description 2
- RBQDIMNMXLVMBS-UHFFFAOYSA-L disodium;2,3-dihydroxybutanedioate;hydrate Chemical compound O.[Na+].[Na+].[O-]C(=O)C(O)C(O)C([O-])=O RBQDIMNMXLVMBS-UHFFFAOYSA-L 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 238000007086 side reaction Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 2
- WQEVDHBJGNOKKO-UHFFFAOYSA-K vanadic acid Chemical compound O[V](O)(O)=O WQEVDHBJGNOKKO-UHFFFAOYSA-K 0.000 description 2
- 206010003645 Atopy Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000007824 enzymatic assay Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- HVHKMUMXERBUAN-IFADSCNNSA-N mesobilirubin IXalpha Chemical compound N1C(=O)C(CC)=C(C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C)C(=O)N\3)CC)N2)CCC(O)=O)N1 HVHKMUMXERBUAN-IFADSCNNSA-N 0.000 description 1
- ALTWGIIQPLQAAM-UHFFFAOYSA-N metavanadate Chemical compound [O-][V](=O)=O ALTWGIIQPLQAAM-UHFFFAOYSA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 238000010850 salt effect Methods 0.000 description 1
- 231100000004 severe toxicity Toxicity 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- LSGOVYNHVSXFFJ-UHFFFAOYSA-N vanadate(3-) Chemical compound [O-][V]([O-])([O-])=O LSGOVYNHVSXFFJ-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
实测均值(n=20) | 本发明试剂盒 | 对比例试剂盒 |
罗氏高值(38.3μmol/L) | 38.5μmol/L | 39.2μmol/L |
CV% | 1.3% | 1.6% |
罗氏低值(13.7μmol/L) | 13.9μmol/L | 14.6μmol/L |
CV% | 2.1% | 3.8% |
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2006101544817A CN1967252B (zh) | 2006-10-30 | 2006-10-30 | 直接胆红素测定试剂盒 |
Applications Claiming Priority (1)
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CN2006101544817A CN1967252B (zh) | 2006-10-30 | 2006-10-30 | 直接胆红素测定试剂盒 |
Publications (2)
Publication Number | Publication Date |
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CN1967252A CN1967252A (zh) | 2007-05-23 |
CN1967252B true CN1967252B (zh) | 2011-11-02 |
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CN2006101544817A Active CN1967252B (zh) | 2006-10-30 | 2006-10-30 | 直接胆红素测定试剂盒 |
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Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101963611B (zh) * | 2009-07-23 | 2014-08-13 | 深圳迈瑞生物医疗电子股份有限公司 | 一种临床检验试剂、试剂盒及方法 |
CN102226768B (zh) * | 2011-03-17 | 2012-10-03 | 郑州兰森生物技术有限公司 | 亚硝酸钠氧化法测定总胆红素的试剂 |
CN102226769A (zh) * | 2011-03-17 | 2011-10-26 | 郑州兰森生物技术有限公司 | 亚硝酸钠氧化法测定直接胆红素的试剂 |
CN103048282B (zh) * | 2012-10-26 | 2015-04-15 | 潍坊鑫泽生物科技有限公司 | 一种胆红素的检测方法及检测试剂盒 |
CN104048928A (zh) * | 2013-03-11 | 2014-09-17 | 南京澳林生物科技有限公司 | 直接胆红素检测试剂盒 |
CN104406971B (zh) * | 2014-11-28 | 2017-03-08 | 山东博科生物产业有限公司 | 一种直接胆红素检测试剂 |
CN105092573A (zh) * | 2015-09-02 | 2015-11-25 | 郁东 | 一种新型的直接胆红素检测试剂盒 |
CN108426761B (zh) * | 2017-02-15 | 2021-11-05 | 上海瀚联医疗技术股份有限公司 | 一种磷酸盐缓冲液及其制备方法 |
CN108613976B (zh) * | 2018-06-06 | 2021-04-09 | 临安卡尔生物技术有限公司 | 直接胆红素检测试剂盒 |
CN109813918B (zh) * | 2019-01-11 | 2021-04-09 | 河北省药品医疗器械检验研究院 | 一种总胆红素测定试剂盒 |
CN112986584A (zh) * | 2021-02-23 | 2021-06-18 | 潍坊泽成生物技术有限公司 | 总胆红素测定试剂盒的制作方法 |
CN113238060B (zh) * | 2021-05-08 | 2022-10-11 | 迈克生物股份有限公司 | 用于预测或诊断心肌炎的试剂盒 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4078892A (en) * | 1975-06-30 | 1978-03-14 | Becton, Dickinson And Company | Novel means and method for diagnostic quantitation of serum or plasma bilirubin |
DK214484A (da) * | 1982-08-27 | 1984-04-27 | Beckman Instruments Inc | Fremgangsmaade til maaling af ''direkte bilirubin'' |
CN1034999A (zh) * | 1988-01-28 | 1989-08-23 | 电核物理有限公司 | 溶液中胆红素的测定方法 |
CN1155583A (zh) * | 1995-10-27 | 1997-07-30 | 协和梅迪克斯株式会社 | 胆红素的定量方法 |
EP1046914A1 (en) * | 1999-04-21 | 2000-10-25 | Mohammad Zouheir Dr. Habbal | Method and reagents for the determination of bilirubins |
-
2006
- 2006-10-30 CN CN2006101544817A patent/CN1967252B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4078892A (en) * | 1975-06-30 | 1978-03-14 | Becton, Dickinson And Company | Novel means and method for diagnostic quantitation of serum or plasma bilirubin |
DK214484A (da) * | 1982-08-27 | 1984-04-27 | Beckman Instruments Inc | Fremgangsmaade til maaling af ''direkte bilirubin'' |
CN1034999A (zh) * | 1988-01-28 | 1989-08-23 | 电核物理有限公司 | 溶液中胆红素的测定方法 |
CN1155583A (zh) * | 1995-10-27 | 1997-07-30 | 协和梅迪克斯株式会社 | 胆红素的定量方法 |
EP1046914A1 (en) * | 1999-04-21 | 2000-10-25 | Mohammad Zouheir Dr. Habbal | Method and reagents for the determination of bilirubins |
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Publication number | Publication date |
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CN1967252A (zh) | 2007-05-23 |
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Owner name: NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO., LTD. Free format text: FORMER NAME: MEIKANG BIOTECH CO., LTD., NINGBO |
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Address after: 315104 Zhejiang city of Ningbo province Yinzhou District Qiming Road No. 299 Patentee after: NINGBO MEDICAL SYSTEM BIOTECHNOLOGY Co.,Ltd. Address before: 315104 Zhejiang city of Ningbo province Yinzhou District Qiming Road No. 299 Patentee before: Ningbo Medical System Biotechnology Co.,Ltd. |
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Address after: 315104 Zhejiang city of Ningbo province Yinzhou District Qiming Road No. 299 Patentee after: MEDICAL SYSTEM BIOTECHNOLOGY Co.,Ltd. Address before: 315104 Zhejiang city of Ningbo province Yinzhou District Qiming Road No. 299 Patentee before: NINGBO MEDICAL SYSTEM BIOTECHNOLOGY Co.,Ltd. |
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Application publication date: 20070523 Assignee: Ningbo Shengyuan Biotechnology Co., Ltd. Assignor: MEDICAL SYSTEM BIOTECHNOLOGY Co.,Ltd. Contract record no.: X2022330000361 Denomination of invention: Direct Bilirubin Assay Kit Granted publication date: 20111102 License type: Common License Record date: 20220809 |
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