CN1903280A - Yukangsan herb medicine powder for treating fish diseases - Google Patents
Yukangsan herb medicine powder for treating fish diseases Download PDFInfo
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- CN1903280A CN1903280A CN 200610048483 CN200610048483A CN1903280A CN 1903280 A CN1903280 A CN 1903280A CN 200610048483 CN200610048483 CN 200610048483 CN 200610048483 A CN200610048483 A CN 200610048483A CN 1903280 A CN1903280 A CN 1903280A
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Abstract
A Chinese medicine 'Yukangsan' for preventing and treating the diseases of fish is prepared from 8 Chinese-medicinal materials including rhubarb, scutellaria root, haw, liquorice root, etc.
Description
Technical field: the present invention relates to a kind of aquaculture technology, relating in particular to a kind of is the Yukangsan of formulated, the anti-system fish disease of composition with Chinese medicine.
Background technology: along with developing rapidly of culture fishery and improving constantly of per unit area yield, the improvement of pond environment condition is but strong inadequately, aqiuculrue trouble bacterial disease and viral disease situation are more and more serious, have now become a major issue of continuous puzzlement culture fishery.Chemical drugs commonly used is is in the past prevented and treated fish diseases, some broad ectrum antibiotic of life-time service and chemical synthetic drug such as quinolones and sulfonamides etc., the result not only makes fish generation drug resistance make therapeutic effect undesirable, and medicine is residual at fish body, water body, works the mischief for easily environment and human body.
Summary of the invention: in order to solve the more and more serious problem of situation that aqiuculrue suffers from bacterial disease and viral disease, the purpose of this invention is to provide a kind of cheapness, medicine source wide, have no side effect, Yukangsan of effectively anti-system fish disease and preparation method thereof.
Technical scheme of the present invention realizes in the following manner:
A kind of Yukangsan that is used for the treatment of the bacillary and viral disease of aqiuculrue: it is prepared by the crude drug with following portions by weight:
Radix Et Rhizoma Rhei 80-100 Radix Scutellariae 40-50 Cortex Phellodendri 50-80 Herba Agrimoniae 70-100 Radix Isatidis 40-75 Radix Angelicae Dahuricae 40-50 Fructus Crataegi 8-15 Radix Glycyrrhizae 20-30
The above-mentioned raw materials medicine is selected from the crude drug of right drying or 40～50 ℃ of oven dry.
The above-mentioned raw materials medicine needs to pulverize and cross 60 mesh sieves through undue sample.
The above-mentioned raw materials medicine need be put into mixer, mixes 5 minutes.
Good effect of the present invention is:
1, reportedly unite theory of Chinese medical science and modern pharmacology studied these a few flavor Chinese herbal medicine and not only common fish evil myxobacter, fluorescent utmost point hair bacillus, point-like aerogenesis monospore bacillus, water mo(u)ld etc. had remarkable killing action, and arc reovirus virus etc. is also had good inhibitory effect.
2, this product can not only be prevented and treated bacterial diseasees such as the common erythroderma of various fishes, gill rot, enteritis disease, scale protrusion disease, stigmatosis well, can also effectively prevent and treat viral diseases such as hemorrhagic disease of grass carp and fulminating epidemic diseases etc., and improve the immunity of organisms of fish in addition, effects such as appetite strengthening, growth promotion.
3, verify through secular laboratory test with in the fish pond, prove that this prescription has comparatively ideal prevention and therapeutic effect to common fish diseases, and its antibiotic enhancing immunity aspect is better than present common chemical medicine within the specific limits, and Chinese herbal medicine avirulence, noresidue, the medicine source is wide, cost is low.
4, use meeting of the present invention is cultured to Aquatic product and is brought bigger social benefit, ecological benefits and economic benefit.
The invention will be further described below in conjunction with embodiment:
Embodiment one:
The Radix Et Rhizoma Rhei 100 gram Radix Scutellariaes 50 gram Cortex Phellodendris 70 gram Herba Agrimoniaes 70 gram Radix Isatidiss 75 gram Radixs Angelicae Dahuricae 40 Keshan short, bristly hair or beards 15 gram Radix Glycyrrhizaes 30 grams
1, selects materials: select the quality raw materials medical herbs according to prescription, make natural drying or 40～50 ℃ of oven drying at low temperatures.
2, pulverize: each the dry back of flavor crude drug divides sample to pulverize in will writing out a prescription, and crosses 60 mesh sieves, divides sample pack label standby then.It is clear to make sure to keep in mind the certain labelling of various kinds nomenclature of drug, in order to avoid mix.
3, mix: put into mixer after the medicine of respectively distinguishing the flavor of that will crush takes by weighing by recipe quantity is proportional, mixed 5 minutes.
4, check: extract the sample that mixes and send the laboratory items such as its character, composition, degree of grinding to be carried out comprehensive test by prescription.
5, packing: semi finished package is after the assay was approved put in storage.
Embodiment two:
The Radix Et Rhizoma Rhei 80 gram Radix Scutellariaes 40 gram Cortex Phellodendris 80 gram Herba Agrimoniaes 100 gram Radix Isatidiss 40 gram Radixs Angelicae Dahuricae 50 Keshan short, bristly hair or beards 8 gram Radix Glycyrrhizaes 20 grams
Preparation technology is with embodiment one
Embodiment three:
The Radix Et Rhizoma Rhei 90 gram Radix Scutellariaes 45 gram Cortex Phellodendris 60 gram Herba Agrimoniaes 80 gram Radix Isatidiss 55 gram Radixs Angelicae Dahuricae 45 Keshan short, bristly hair or beards 10 gram Radix Glycyrrhizaes 25 grams
Preparation technology is with embodiment one
Intermediate experiment of fish body and production checking
(1), fish body intermediate experiment
1, this product is prevented and treated test to Ctenopharyngodon idellus " three diseases ":
(1) test material: with 4 glass aquariums of 100,000 milliliters of being filled with water, specification be 50 * 50 * 50 (centimetre), load onto the tap water behind 24 hours aerations.Remove contamination with siphonage every day in the test, changes water twice, and each quantity of exchanged water is 1/5 of a Total Water, and water temperature 23-28 ℃, PH7-7.5, the Ctenopharyngodon idellus fingerling of raising susceptible.
(2) test method:, be respectively charged into number consecutively and be in 1,2,3, No. 4 the aquarium with each 30 tail of the Ctenopharyngodon idellus of natural occurrence.Phenomenons such as extract the Ctenopharyngodon idellus with batch natural occurrence, blackout appears in visual inspection fish body, and head is pitch-black, and mouth hyperemia, the gill are pale, and scale comes off, and tail fin is disconnected.Cut open inspection back and find that the gill filament rots, or have cartilage exposed, there is yellow mucus in the abdominal cavity, and intestinal is empty and rot to be aubergine.Diagnosing the disease fish through Agricultural University Of He'nan's Animal Lab. is by fluorescent utmost point hair bacillus, point-like aerogenesis monospore bacillus, red skin, enteritis and gill rot " three diseases " intercurrent disease that the mixed infection of fish evil myxobacter causes.
(3) amount to calculating according to " Chinese materia medica for animals " medium domestic animal amount, and use the powder feeding volume with reference to fish inside and outside the province, preceding 6 days is 0.5%, 1%, 3% pharmaceutical chemistry with throwing something and feeding to containing this powder in 1,2, No. 3 aquarium respectively, throws something and feeds the contrast bait of throwing something and feeding after 6 days every day 2 times.And the 30 tail fishes contrast bait of always throwing something and feeding in No. 4 casees.
(4) above each group test is all observed and was raised 15 days, and record fish body is ingested and dead, survival condition.
(5) result of the test: result of the test sees Table 1
Table 1 dosage screening test result
| Dosage (case number (CN)) | Death toll | The survival number | Sum | Mortality rate |
| 0.5% pharmaceutical chemistry (No. 1) | 20 | 10 | 30 | 66.7％ |
| 1% pharmaceutical chemistry (No. 2) | 12 | 18 | 30 | 40.00％ |
| 3% pharmaceutical chemistry (No. 3) | 10 | 20 | 30 | 33.33％ |
| Blank (No. 4) | 28 | 2 | 30 | 93.33％ |
With 0.5% pharmaceutical chemistry and 1% bait feeding effect extremely significant difference P＜0.01 is arranged as can be seen by the check of pharmacognosy statistical independence.We can draw with 1% pharmaceutical chemistry and 3% bait feeding effect no significant difference P＞0.05,1% bait feeding and the pharmaceutical chemistry group of not throwing something and feeding with quadrat method extremely significant difference P＜0.01, so we determine that 1% pharmaceutical chemistry oral dosage is preferable medicine recommended amounts.
By above experiment we as can be seen this medicine significant curative effect is being arranged in control aspect the three major diseases of grass carp.With we have also done same experiment to the Cyprinus carpio of natural occurrence, silver carp, Aristichthys nobilis etc. with quadrat method, find that this product can not only treat Ctenopharyngodon idellus " three diseases ", also can prevent and treat the fulminant epidemic diseases, enteritis disease, gill rot, stigmatosis, white skin disease, scale protrusion disease of Fish etc.
2, medicine contrast experiment:
(1) trial drug: " Yukangsan " and " furazolidone "
(2) experimental technique:
A, each 30 tail of the Ctenopharyngodon idellus of natural occurrence are placed on respectively in 1,2, No. 3 aquarium.
The content of dispersion of throwing something and feeding is the pharmaceutical chemistry of per 50 kilogram of 1 gram furazolidone preceding 6 day every day in b, No. 1 aquarium, changes later on and feeds contrast bait; In No. 3 aquariums is the blank case, the contrast bait of only throwing something and feeding and not containing medicine.Each group was all observed 15 days.
(3) experimental result sees Table 2
Table 2 medicine contrast and experiment
| Group | Death toll | The survival number | Sum | Mortality rate |
| 1% medium-height grass pharmaceutical chemistry (1) | 10 | 20 | 30 | 33.33％ |
| Furazolidone (2) | 12 | 18 | 30 | 40％ |
| Contrast (3) | 26 | 4 | 30 | 83.33％ |
The oral medicine commonly used " furazolidone " of " Yukangsan " and fish is compared identical therapeutical effect is arranged as can be seen from Table 2, even curative effect is also better than furazolidone.
(2), produce checking (production experiment)
1, test period: 1994-1996 Fish onset peak period.
2, test site and pond area:
Select to suffer from 1 on the Ctenopharyngodon idellus kind pool of Ctenopharyngodon idellus " four diseases " (hemorrhagic disease, erythroderma, enteritis disease, gill rot) in August, (1) 1994 in Jinshui District Yao Qiao township, Zhengzhou City, 4 mu of pond areas are No. 1 test pool.
(2) nineteen ninety-five JIUYUE to hold a memorial ceremony at Zhengzhou City's Jinshui District that town and country select with silver carp, flathead be that the natural occurrence (stigmatosis, gill rot) of main body fish is raised together with the fish pond, 8 mu of pond areas are No. 2 test pools.
Selecting with the Cyprinus carpio in Jinshui District Yao Qiao township, Zhengzhou City (3) 1996 year 6-9 month is 10 on the production pool of main body fish, and each fish pond area is 10 mu, and 100 mu of the gross areas are No. 3 test pools.
3, test method:
(1) adopting 1% pharmaceutical chemistry for oral administration and external concentration on No. 1 test pool is 1 * 10
-6The method that this medicine full pool spilling head of (not allowed with this unit of PPM now) combines, once a day, continuous 4 days.
(2) only adopting concentration on No. 2 pools is 1 * 10
-6The method of this product medicine full pool spilling head, every day 1 time, logotype 4 days.
(3) done the chemoprophylaxis test on No. 3 test pools.Adopting 1% pharmaceutical chemistry for oral administration and full pool spilling head concentration is 0.5 * 10
-6Medicine was used alternatingly 1 time method in per 20 days.
4, result of the test:
(1) No. 1 pool therapeutic outcome of Ctenopharyngodon idellus " four diseases " natural occurrence sees Table 3
Sick fish death record table before and after the medication of No. 1 pool of table 3
| The date moon/day | Preceding 3 days of medication | During the medication (4 days) | After the medication (4 days) |
| 8/11 8/12 8/13 | 8/14 8/15 8/16 8/17 | 8/18 8/19 8/20 8/21 | |
| Dead mantissa | 11 15 21 | 20 11 3 1 | 0 0 0 0 |
Result of the test showed medication after 3 days, and sick fish death toll begins to descend, and sb.'s illness took a favorable turn, treated a complete disease controlling course of treatment (after 3 days).
(2) No. 2 test pool therapeutic outcome sees Table 4
Sick fish death record table before and after the medication of No. 2 pools of table 4
| The date moon/day | Preceding 3 days of medication | During the medication (4 days) | After the medication (4 days) |
| 9/3 9/4 9/5 | 9/6 9/7 9/8 9/9 | 9/10 9/11 9/12 9/13 | |
| Dead mantissa | 3 5 4 | 4 2 1 1 | 0 0 0 0 |
Result of the test shows only treats with the full pool spilling head method the lighter bacterial diseases of fishes of the state of an illness, and its curative effect is also very remarkable.
(3) respond well to the prophylactic tria on No. 3 test pools, not only serious fish diseases does not take place in fish diseases onset peak period (the 6-9 month), and annual other serious fish diseasess that all do not take place.
Claims (4)
1, a kind of Yukangsan that is used for the treatment of fish disease, it is prepared by the crude drug with following portions by weight:
Radix Et Rhizoma Rhei 80-100
Radix Scutellariae 40-50
Cortex Phellodendri 50-80
Herba Agrimoniae 70-100
Radix Isatidis 40-75
Radix Angelicae Dahuricae 40-50
Fructus Crataegi 8-15
Radix Glycyrrhizae 20-30.
2, the Yukangsan that is used for the treatment of fish disease according to claim 1, it is characterized in that: crude drug is selected from the crude drug of right drying or 40～50 ℃ of oven drying at low temperatures.
3, the Yukangsan that is used for the treatment of fish disease according to claim 2 is characterized in that: crude drug needs to pulverize and cross 60 mesh sieves through undue sample.
4, the Yukangsan that is used for the treatment of fish disease according to claim 1, it is characterized in that: crude drug need be put into mixer, mixes 5 minutes.
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| CNB2006100484838A CN100462089C (en) | 2006-07-28 | 2006-07-28 | Yukangsan herb medicine powder for treating fish diseases |
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| CNB2006100484838A CN100462089C (en) | 2006-07-28 | 2006-07-28 | Yukangsan herb medicine powder for treating fish diseases |
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| CN100462089C CN100462089C (en) | 2009-02-18 |
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