CN103800634A - Chinese herbal medicine feed additive for controlling main bacterial infectious diseases of freshwater fish - Google Patents
Chinese herbal medicine feed additive for controlling main bacterial infectious diseases of freshwater fish Download PDFInfo
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- CN103800634A CN103800634A CN201410040027.3A CN201410040027A CN103800634A CN 103800634 A CN103800634 A CN 103800634A CN 201410040027 A CN201410040027 A CN 201410040027A CN 103800634 A CN103800634 A CN 103800634A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention relates to a Chinese herbal medicine feed additive for controlling main bacterial infectious diseases of freshwater fish. The Chinese herbal medicine feed additive is prepared from the following raw materials in parts by weight: 3-5 parts of purslane, 3-4 parts of dahurian patrinia herb, 2-3 parts of erodium stephanianum willd, 1-2 parts of Chinese gall, 1-2 parts of rosa laevigata michx, 1-2 parts of garlic and 1-2 parts of liquorice. The Chinese herbal medicine feed additive for controlling the main bacterial infectious diseases of the freshwater fish is used for controlling the main bacterial infectious diseases of the freshwater fish, and has the characteristics of being high in cure rate, good in effect, safe, free from toxic and side effects and convenient to take.
Description
Technical field
The present invention relates to a kind of Chinese herbal feed additive of preventing and treating fresh-water fishes main bacteria sexually transmitted disease.
Background technology
Fresh-water fishes mainly contain Ctenopharyngodon idellus, tilapia, Hypophthalmichthys molitrix, Mylopharyngodon piceus, Carassius auratus, Megalobrama amblycephala, tilapia etc. more than 40 and plant, and main bacteria sexually transmitted disease has bacterial septicemia, enteritis disease, white head and mouth fish disease, gill rot, erythroderma etc.Its pathogen mainly contains Aeromonas, myxobacter, has a liking for the glutinous antibacterial of fiber, pseudomonas fluorescens etc.At present, preventing and treating such bacterial infectious disease is mainly to use antibiotic and chemicals, but life-time service antibiotic and chemicals be polluted-water environment not only, and reduction fish body natural immunity, fish body is caused and injured, form drug residue, make disease more be difficult to control, aquatic product quality declines; That Chinese herbal medicine has is natural, have no side effect,, the feature such as be difficult for develop immunity to drugs residual without chemicals, for the diseases preventions of fresh-water fishes, cure the disease, is with a wide range of applications.Although prior art discloses the Chinese herbal and crude drugs preparations for preventing and treating freshwater fish bacterial infectious disease, exist cost high, the problem such as therapeutic effect is poor.
Summary of the invention
The object of the invention is to for the problems referred to above, the Chinese herbal feed additive of low, the effective control fresh-water fishes main bacteria sexually transmitted disease of a kind of use cost is provided.
Technical scheme of the present invention is: a kind of Chinese herbal feed additive of preventing and treating fresh-water fishes main bacteria sexually transmitted disease, and it is to be made up of the raw material of following weight parts: Herba Portulacae 3-5 part, Herba Patriniae 3-4 part, geranium 2-3 part, Galla Chinensis 1-2 part, Fructus Rosae Laevigatae 1-2 part, Bulbus Allii 1-2 part, Radix Glycyrrhizae 1-2 part;
Above-mentioned raw materials is ground into respectively to coarse powder, mixes, pack, obtain the Chinese herbal feed additive of this control fresh-water fishes main bacteria sexually transmitted disease.
The preferred feedstock weight portion of making the Chinese herbal feed additive of this control fresh-water fishes main bacteria sexually transmitted disease is: 4 parts of Herba Portulacaes, 4 parts of Herba Patriniae, 2 parts of geranium, 2 parts of Galla Chinensiss, 1.5 parts of Fructus Rosae Laevigatae, 1.5 parts, Bulbus Allii, 1 part, Radix Glycyrrhizae.
The Chinese herbal feed additive of this control fresh-water fishes main bacteria sexually transmitted disease adds in bait and uses.
In the Chinese herbal feed additive prescription of this control fresh-water fishes main bacteria sexually transmitted disease, each component pharmacological effect is:
Herba Portulacae effect: Herba Portulacae another name long life dish, five elements' oxalic acid taste dish, longevity greens/mustard green etc.Cold in nature, sour in the mouth, return large intestine, liver, spleen channel.Contain noradrenalin and volume potassium salt potassium nitrate, potassium chloride, potassium sulfate etc.Still containing dihydroxy benzenes ethamine, dihydroxyphenylalanine, malic acid, citric acid, glutamic acid etc.There is effect of heat-clearing and toxic substances removing, for bacillary dysentery etc.
Herba Patriniae effect: Herba Patriniae another name gutweed, Pan Ercao, bitter ball dish, native Herba Taraxaci, Radix hemerocalis plicatae, Herba Lactucae formosanae dish etc.Bitter in the mouth, cold in nature, returns stomach, large intestine, Liver Channel.Containing oleanolic acid, helexin, Hemerocallis citrina Baroni dragon bud glycosides, daucosterol and multiple saponin; Still containing volatile oil, wherein the highest with patrinene and isopatrinene content; Also containing alkaloid, tannin etc.Herba Patriniae has inhibitory action to staphylococcus aureus, dysentery bacterium, Bacillus typhi, bacillus pyocyaneus, escherichia coli.Effect of tool heat-clearing and toxic substances removing, reducing swelling and alleviating pain.For jaundice, dysentery, stranguria etc.
Geranium effect: geranium is called old stork mouth, crow mouth, passes through muscle, always passed through muscle, old tendon Bovis seu Bubali etc.Property hardship, acrid in the mouth, return liver,kidney,spleen warp.Choose glycoside containing tannin, spun gold.Staphylococcus aureus, shigella flexneri, group B streptococcus etc. are had to obvious inhibitory action.Effect of tool expelling wind and activating blood circulation, heat-clearing and toxic substances removing.For rheumatalgia, contracture numbness, enteritis and dysentery etc.
Galla Chinensis effect: Galla Chinensis another name Fructus rhois chinensis, mountain Firmiana platanifolia (Linn. f.) Marsili, five times of bavins, non-irrigated gall nut, black Folium Persicae etc.
Sour-puckery flavor, cold in nature, returns lung, large intestine, kidney channel.Containing tannin, tannic acid has precipitation to protein, and typhoid fever, paratyphoid fever, dysentery etc. are all had to significantly antibacterial or bactericidal action.Astringe the lung effect of pathogenic fire reducing, relieving diarrhea with astringents, astringing to arrest bleeding, removing dampness sore of tool.For diseases such as chronic diarrhea chronic dysentery, the hemorrhoidal bleeding of having blood in stool, eczema toxic swellings.
Fructus Rosae Laevigatae effect: Fructus Rosae Laevigatae another name Cortex Hemipteleae Davidii, thorn pear, golden small-mouthed jar, Catunaregam spinosa (Thunb.) Tirveng., Gallus jabouillei chieftain etc., sour in the mouth, sweet, property is puckery, flat.Return kidney, bladder, large intestine channel.The compositions such as sugary, citric acid, malic acid, tannin, vitamin, more than 20 seed amino acids, 18 kinds of mineral elements and resin, Saponin.Effect of tool astringing intestine to stop diarrhea controlling nocturnal emission with astringent drugs, stopping leak dysentery.For diseases such as spermatorrhea seminal emission, frequent micturition, insufficiency of the spleen chronic diarrhea.
Bulbus Allii effect: Bulbus Allii another name Bulbus Allii, Bulbus Allii, DUSUAN, giant garlic etc.Sweet in the mouth, warm in nature, pungent, enter spleen, stomach, lung meridian.Containing protein, fat, carbohydrate, calcium, phosphorus, ferrum, vitamin C.Also contain the trace element such as thiamine, riboflavin, nicotinic acid, allicin, citral and selenium and germanium.Containing volatile oil, in oil, main component is allicin, has bactericidal action.The stomach invigorating of tool warming middle-JIAO, help digestion regulate the flow of vital energy, effect of parasite killing removing toxic substances, antidiarrheal dysentery relieving.For flu, bacillary dysentery, amebic dysentery, enteritis, eating accumulation, carbuncle skin infection.
Radix Glycyrrhizae effect: Radix Glycyrrhizae another name Glycyrrhizae, close grass, state are old, Herba Hedyotis cantonensis, sweet root etc.Sweet in the mouth, property are put down, and return spleen, stomach, lung meridian.Containing compositions such as glycyrrhizic acids, glycyrrhizin, licoflavone, glycyrrhizin, isoliquiritigenin, Angelica Polysaccharide, there is antiulcer, regulate the effect such as phagocytic function of macrophage.Glycyrrhizic acid is natural antidote, can be used for enhancing immunity, maintains water-electrolyte metabolism balance etc.There is effect of invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.For weakness of the spleen and stomach, fatigue and weakness, carbuncle sore tumefacting virus, cushion toxicity, strong.
Prescription foundation of the present invention: in side, Herba Portulacae, Herba Patriniae, geranium have heat-clearing and toxic substances removing, antibacterial and anti-inflammation functions, and antimicrobial spectrum is wider is effective to multiple gram positive bacteria, gram-negative bacteria; Galla Chinensis, Fructus Rosae Laevigatae are stopped chronic dysentery, thick the intestines and stomach, spleen invigorating in tune; Herba Patriniae, Bulbus Allii contain volatile oil, pungent loose logical temperature, and fragrant odour, can regulate the flow of vital energy into spleen lung, promoting the circulation of QI to alleviate the stagnation in middle-JIAO.Bulbus Allii also all has certain inhibitory action to dysentery bacterium, Bacillus typhi, vibrio cholera, escherichia coli, Bacillus proteus, bacillus pyocyaneus and staphylococcus, streptococcus etc.Bulbus Allii, Radix Glycyrrhizae have food calling, promoting digestion and removing stagnation effect, and Radix Glycyrrhizae regulates body's immunity, sweet temperature QI invigorating, current meridians, coordinating the actions of various ingredients in a prescription.In side all medicine phases and, play altogether anti-inflammation, promoting digestion and removing stagnation, antidiarrheal dysentery relieving, improve effect of immunity of organisms and resistance against diseases.Be applicable to prevent and treat fresh-water fishes main bacteria sexually transmitted disease.
The Chinese herbal feed additive of this control fresh-water fishes main bacteria sexually transmitted disease, is having carried out on the basis of a large amount of practical applications, according to traditional medicine ancient prescription, forms in conjunction with physiology of fishes, infectious disease nosetiology and immunologic theoretical screening.The Chinese herbal feed additive of this control fresh-water fishes main bacteria sexually transmitted disease can be given full play to effect of each raw material in component, and the used time admixes in feedstuff, granulation bait feeding fresh-water fishes.The present invention is used for the treatment of fresh-water fishes main bacteria sexually transmitted disease, short treating period, treatment cost is low, cure rate is high, safely, have no side effect, easy to use.
The specific embodiment
Below in conjunction with embodiment, the invention will be further described.
1, take respectively raw material (herbal decoction piece): Herba Portulacae 250 g, Herba Patriniae 250 g, geranium 125 g, Galla Chinensis 125 g, Fructus Rosae Laevigatae 93.75 g, Bulbus Allii 93.75 g, Radix Glycyrrhizae 62.5 g;
2, above-mentioned raw materials is ground into respectively to coarse powder, mixes, stir evenly, subpackage, makes the Chinese herbal feed additive of this control fresh-water fishes main bacteria sexually transmitted disease.When use, the Chinese herbal feed additive of this control fresh-water fishes main bacteria sexually transmitted disease mixes rear granulation shape bait feeding with mash feed.
Some test situation of utilizing the Chinese herbal feed additive of this control fresh-water fishes main bacteria sexually transmitted disease to carry out are as follows: tilapia belongs to fresh-water fishes, the normal bacterial septicemia of sending out in breeding process, its cause of disease mostly is Aeromonas sobria, and Chinese herbal feed additive of the present invention is tested as an example of the bacterial septicemia of control tilapia Aeromonas sobria example.
(1) Chinese herbal feed additive of the present invention is measured Aeromonas sobria bacteriostatic diameter, minimum inhibitory concentration, minimum bactericidal concentration: adopt agar diffusion method of the paper to measure susceptibility diameter, every single is 20mg for the drug sensitive test paper content of dispersion of examination Chinese herbal medicine and Chinese herbal feed additive of the present invention, takes out the pastille scraps of paper that prepare be attached to and be coated with Aeromonas sobria (bacterial concentration 7.5 X 10 with sterilizing tweezers with sterile working
5) plating medium surface, the test of every kind of tablet repeats 3 times.In 28 ℃ of constant incubators, cultivate after 20-24 h observed result.
Measure the minimum inhibitory concentration of Chinese herbal feed additive of the present invention to Aeromonas sobria, the highly diluted Guan Zhongwu bacterial growth person of every medicine, the concentration of this pipe is the minimum inhibitory concentration of this kind of medicine to this bacterial strain; Measure the minimum bactericidal concentration of Chinese herbal feed additive of the present invention to Aeromonas sobria, on Minimal inhibitory concentration basis, from drawing materials and coat respectively Agar Plating without each pipe of bacterial growth, in 28 ℃ of constant incubators, cultivate after 24-48h observed result.On agar plate, contain 1 minimum pipe of medicinal liquid without bacterial growth, be the minimum bactericidal concentration of this kind of medicine to this bacterial strain.Chinese herbal feed additive of the present invention to Aeromonas sobria bacteriostatic diameter, minimum inhibitory concentration, minimum bactericidal concentration in table 1.
Table 1 Chinese herbal medicine is to Aeromonas sobria bacteriostatic diameter, minimum inhibitory concentration, minimum bactericidal concentration unit: mm mg/mL
| Chinese herbal medicine | 0.5% group antibacterial | 1.0% group antibacterial | 1.5% group antibacterial | Minimum antibacterial | Minimum bactericidal |
| ? | Diameter | Diameter | Diameter | Concentration | Concentration |
| Herba Portulacae | 20.5 | 21.0 | 22.5 | 1.80 | 1.95 |
| Herba Patriniae | 19.5 | 20.0 | 20.5 | 1.95 | 2.15 |
| Geranium | 21.0 | 21.5 | 22.5 | 2.10 | 2.36 |
| Galla Chinensis | 19.0 | 20.5 | 21.5 | 2.26 | 2.45 |
| Fructus Rosae Laevigatae | 18.0 | 19.0 | 19.5 | 2.78 | 2.89 |
| Bulbus Allii | 21.0 | 21.5 | 22.0 | 1.95 | 2.40 |
| Radix Glycyrrhizae | 15.0 | 15.5 | 15.5 | 2.21 | 2.50 |
| Chinese herbal feed additive of the present invention | 23.5 | 25.0 | 26.5 | 1.65 | 1.86 |
This result of study shows: each single Chinese herbal medicine all has certain bacteriostasis, and bacteriostatic diameter raises with the rising of drug level, and wherein Herba Portulacae, Herba Patriniae and geranium, Bulbus Allii fungistatic effect are better, and Galla Chinensis, Fructus Rosae Laevigatae also have certain fungistatic effect.Relatively bacteriostatic diameter is the highest for Chinese herbal feed additive of the present invention and each single Chinese herbal medicine, more than reaching 23mm.0.5%, 1.0%, 1.5% group of Chinese herbal feed additive of the present invention is respectively 23.5mm, 25.0mm, 26.5mm to the bacteriostatic diameter of Aeromonas sobria.Chinese herbal feed additive of the present invention not only can suppress the Growth and Reproduction of pathogen Aeromonas sobria, but also can directly kill pathogen Aeromonas sobria.Chinese herbal feed additive minimum inhibitory concentration 1.65mg/mL of the present invention, minimum bactericidal concentration 1.86mg/mL, with each single Chinese herbal medicine comparison minimum inhibitory concentration and minimum bactericidal concentration numerical value be minimum, effect is best, illustrates that in Chinese herbal feed additive prescription of the present invention, seven taste Chinese herbal medicine " Herba Portulacae, Herba Patriniae, geranium, Galla Chinensis, Fructus Rosae Laevigatae, Bulbus Allii, Radix Glycyrrhizae " compatibility has synergism.
(2) test of pesticide effectiveness of Chinese herbal feed additive of the present invention to tilapia artificial challenge Aeromonas sobria disease: tilapia is supported after several days, in every tail fish pectoral fin lumbar injection 0.2mL of place Aeromonas sobria suspension (bacterial concentration 7.5 X 10 temporarily
5).The pharmaceutical chemistry of the various dose of not adding and adding 0.5%, 1.0%, 1.5% group of Chinese herbal feed additive of the present invention of throwing something and feeding respectively, feeding rate is 1.8% of tested fish body weight.Throw something and feed continuously after 7d pharmaceutical chemistry, observe tilapia Cure.The result of the test that Chinese herbal feed additive of the present invention infects Aeromonas sobria drug effect to tilapia is in table 2.
Table 2 Chinese herbal feed additive of the present invention infects Aeromonas sobria test of pesticide effectiveness result unit to tilapia: tail %
This result of study shows: the mortality rate that does not add Chinese herbal feed additive group tilapia of the present invention is the highest, be 80%, in test, dead fish shows typical bacterial septicemia symptom mostly, that fish body shows as is hemorrhage, anus outstanding, rotten tail, and the mortality rate that adds Chinese herbal feed additive group tilapia of the present invention is lower, have respectively compared with high curative rate.Chinese herbal feed additive addition of the present invention is that 0.5% group of cure rate reaches 84%, 1.0% group of cure rate and reaches 90%, 1.5% group of cure rate and reach 82%.Pharmaceutical chemistry in this test adopts full medicine addition manner preparation, 0.5% group is not remarkable with the pharmaceutical chemistry prevention effect difference of 1.0% group of Chinese herbal feed additive group of the present invention, but dosage is that 1.5% group of Chinese herbal feed additive pharmaceutical chemistry flavour of a drug of the present invention is too heavy, olfactory sensation and the mouthfeel of tilapia are affected, cause feeding rate decreased, reduce prevention effect, cure rate only 82%, and increased drug cost.Therefore, suggestion dosage with 1.0% group of Chinese herbal feed additive of the present invention on producing is prepared pharmaceutical chemistry.
Claims (2)
1. prevent and treat a Chinese herbal feed additive for fresh-water fishes main bacteria sexually transmitted disease, it is characterized in that it is to be made up of the raw material of following weight parts: Herba Portulacae 3-5 part, Herba Patriniae 3-4 part, geranium 2-3 part, Galla Chinensis 1-2 part, Fructus Rosae Laevigatae 1-2 part, Bulbus Allii 1-2 part, Radix Glycyrrhizae 1-2 part;
Above-mentioned raw materials is ground into respectively to coarse powder, mixes, pack, obtain the Chinese herbal feed additive of this control fresh-water fishes main bacteria sexually transmitted disease.
2. the Chinese herbal feed additive of control fresh-water fishes main bacteria sexually transmitted disease according to claim 1, is characterized in that it is to be made up of the raw material of following weight parts: 4 parts of Herba Portulacaes, 4 parts of Herba Patriniae, 2 parts of geranium, 2 parts of Galla Chinensiss, 1.5 parts of Fructus Rosae Laevigatae, 1.5 parts, Bulbus Allii, 1 part, Radix Glycyrrhizae.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104782980A (en) * | 2015-04-21 | 2015-07-22 | 安徽天邦饲料科技有限公司 | Intestine-protecting non-tolerant efficient puffing granular compound herring feed and preparation method thereof |
| CN107549514A (en) * | 2017-10-23 | 2018-01-09 | 无为县雨露生态农业有限公司 | Fresh-water fishes ecologic breeding feed and preparation method thereof |
| CN107568506A (en) * | 2017-10-23 | 2018-01-12 | 无为县雨露生态农业有限公司 | Fresh water fish feed and preparation method thereof |
| CN107581408A (en) * | 2017-10-23 | 2018-01-16 | 无为县雨露生态农业有限公司 | Mixed composite microbial feed for freshwater fish and preparation method thereof |
| CN109350686A (en) * | 2018-11-30 | 2019-02-19 | 吉林农业大学 | A kind of Chinese medicine additive for preventing and treating snakehead nocardiasis |
| CN112656854A (en) * | 2021-02-08 | 2021-04-16 | 集美大学 | Traditional Chinese medicine composition for preventing and treating fish visceral ichthyophthiriasis and preparation method and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104782980A (en) * | 2015-04-21 | 2015-07-22 | 安徽天邦饲料科技有限公司 | Intestine-protecting non-tolerant efficient puffing granular compound herring feed and preparation method thereof |
| CN107549514A (en) * | 2017-10-23 | 2018-01-09 | 无为县雨露生态农业有限公司 | Fresh-water fishes ecologic breeding feed and preparation method thereof |
| CN107568506A (en) * | 2017-10-23 | 2018-01-12 | 无为县雨露生态农业有限公司 | Fresh water fish feed and preparation method thereof |
| CN107581408A (en) * | 2017-10-23 | 2018-01-16 | 无为县雨露生态农业有限公司 | Mixed composite microbial feed for freshwater fish and preparation method thereof |
| CN109350686A (en) * | 2018-11-30 | 2019-02-19 | 吉林农业大学 | A kind of Chinese medicine additive for preventing and treating snakehead nocardiasis |
| CN112656854A (en) * | 2021-02-08 | 2021-04-16 | 集美大学 | Traditional Chinese medicine composition for preventing and treating fish visceral ichthyophthiriasis and preparation method and application thereof |
| CN112656854B (en) * | 2021-02-08 | 2022-08-05 | 集美大学 | Traditional Chinese medicine composition for preventing and treating fish visceral ichthyophthiriasis and preparation method and application thereof |
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| CN103800634B (en) | 2017-05-10 |
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