CN1880442A - Multi-section flat type photo-bioreactor - Google Patents
Multi-section flat type photo-bioreactor Download PDFInfo
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- CN1880442A CN1880442A CNA200610026539XA CN200610026539A CN1880442A CN 1880442 A CN1880442 A CN 1880442A CN A200610026539X A CNA200610026539X A CN A200610026539XA CN 200610026539 A CN200610026539 A CN 200610026539A CN 1880442 A CN1880442 A CN 1880442A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
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Abstract
The invention discloses a flat optical biological reactor, which consists of transparent flat box and gas-supplying device, wherein multiple hollow diversion baffle cavities are set in the box, which contain one or more artificial light source; the cavity is set on the vertical plane in the reactor with gap among each diversion baffle cavity.
Description
Technical field
The present invention relates to bioengineering field, be specifically related to little algae high-density culture field, more particularly relate to a kind of flat bioreactor microalgae culture system of novelty.
Background technology
It is the focus of competitively studying both at home and abroad in recent years that bioreactor is applied to little algae high-efficient culture.At present, the algae large scale culturing is that open type is cultivated substantially, has shortcomings such as easily pollution, difficulty are controlled, efficient is low.In order to overcome above-mentioned shortcoming, people begin one's study and use closed photo bioreactor and carry out the cultivation of little algae, have developed various closed photo bioreactors at present.The base form of bioreactor mainly comprises tubular reactor, column reactor and flat-plate reactor.The basic ideas that adopt during these reactor design are two: 1) reduce light path to increase the luminous energy that each cell can receive; 2) mix fully to guarantee that the suitableeest light intensity can effectively offer cell and strengthen gaseous interchange.
Closed smooth autotrophy culture systems has obtained using widely on little algae is cultivated.Most widely used large scale system (can arrive 1000L) is (the Patrick Lavens of Pig system that Baynes etc. proposed in 1979, PatrickSorgeloos.Mannual on the production and use of live food for aquaculture[M] .FAOFisheries Technical Paper 361,1996), this mainly is because Pig system building cost is low, and easy and simple to handle, the open system culture efficiency is greatly improved relatively.At present domestic main use Pig system carries out secondary seed cultivates, but still there are some problems in the Pig system: after amplifying at container volume, because container diameter is relatively large, make the light application ratio surface-area less relatively, cause the restrictive cultivation of light; Blow and vent system is not smooth, causes algae liquid undercompounding in the container, and oxygen is resolved and can not effectively be carried out etc., and the culture efficiency of frond is had considerable influence.
2000, built up the tight vertical arrangement of a cover at the Wolfsburg of Germany, the tubular type photo-bioreactor system of horizontal flow (O.Pulz.Photobioreactors:production systems for phototrophicmicroorganisms[J] .Appl.Microbiol.Biotechnol., 2001,57:287-293), this reactor is that the Glass tubing of 4cm is made by diameter, length overall 500000m, cumulative volume 700m3, take up an area of 10000m2, produce the chlorella cells dry weight per year and reach the 130-150 ton.AGM (Aquasearch Growth Module) culture systems (the Miguel Olaizola.Commercial production of astaxanthin from Haematococcus pluvialis using 25 that also has the 25000L of Miguel Olaizola report to form in addition by horizontal pipe, 000-liter outdoor photobioreactors[J] .Journal of Applied Phycology, 2000,12:499-506) etc.
At home, units such as Inst. of Hydrobiology, Chinese Academy of Sciences, Jiangxi Province's scientific information research institute and University Of Yantai have all carried out preliminary research work aspect closed photo bioreactor, but the essentially no Controlling System of the bioreactor of being developed.In addition, the glass pipe formula bioreactor of 1000L is applied to the large scale culturing research of chlorella out of doors.In recent years, units such as ocean biochemical engineering institute of East China University of Science, Chinese Academy Of Sciences Process Engineering Research Institute cultivate the light autotrophy of closed photo bioreactor and little algae and have carried out some researchs, successfully develop into the 10-150L series product, realize the steam sterilizing on the throne of bioreactor, had pH, dissolved oxygen, intensity of illumination, the isoparametric online detection of temperature and second computer controlled function.In addition Dalian aquatic product Academy developed the 600L airlift reactor that has inside and outside light source concurrently (Wu Yin, Sun Jianming, Yang Zhiping. airlift photobioreactor is cultivated the pilot scale research [J] of marine microalgae. Transactions of the Chinese Society of Agricultural Engineering, 2004,20 (5): 237-240) etc.
The geometrical shape of bioreactor is one of key factor that influences optical energy utilization efficiency.Duct type bioreactor specific surface area is big, but oxygen is resolved difficulty, and the while is easy cleaning not, is not suitable for the particularly large scale culturing of bait micro-algae of little algae; Though the cylinder shape bioreactor does not exist oxygen to resolve the problem of difficulty, mix also very evenly, amplify difficulty, vertically still radially amplify all very big problems of existence; Flat bioreactor is for the two kinds of reactors in front, have simultaneously that the light application ratio surface-area is big, illumination utilization ratio height, mix, oxygen resolve fast, amplify advantages such as relatively easy, therefore has certain advantage with flat bioreactor as foundation structure, than the plant-scale application of more promising, the easier realization of other type of closed formula bioreactor, therefore numerous investigators studies it, and representative has:
1. the box bioreactor of the plate of source of artificial light (Samson R, Leduy A.Multistagecontinuous cultivation of blue-green alga Spirulina maxima in the flat tankphotobioreactors with recycle[J] .The Canadian Journal of Chemical Engineering, 1985,63:105-112): Canadian Samson and Leduy have reported the box bioreactor of a kind of plate of source of artificial light, plate case length 122cm, the about 50cm of dress liquid height of algae liquid in the plate case thickness 10cm, plate case.With 16 luminescent lamp irradiations, 8 of the every sides in plate case both sides, surperficial average intensity 30klux.The general assembly liquid of reactor amasss 64L.The about 20m-1 of surface-area and volume ratio, the bubbling of taking to ventilate mixes.
2. the board-like bioreactor of horizontal positioned (Ramos de Ortega, Roux J C.Production ofchlorella biomass in different types of flat bioreactors in temperate zones[J] .Biomass, 1986,10:141-156): the outdoor culture studies that the Ramos de Ortega of France and Roux have reported the board-like bioreactor of a kind of hard polyethylene plastics.This reactor is combined by the cell board with three layers of wall, the long 6m of every module unit plate, wide 25cm, thick 4cm.The internal structure of cell board is similar to the internal structure of hollow tile, from the cross-sectional view of cell board, can see the latticed square through hole pipeline of two rows.One row's square pipe is used for leading to water coolant, and another row's square pipe flows as algae liquid.Each square siphunculus length and plate appearance etc. are 6m, and bore is 3.85cm * 1.85cm.
3. vertical caulking groove plate-type reactor (Tredici M R, Carlozzi P, Chini Zittelli G et al.Avertical alveolar panel (VAP) for outdoor mass cultivation of microalgae andcyanobacteria[J] .Bioresource Technology, 1991,38:153-160): gondola Tredici etc. have developed a kind of vertical caulking groove template formula reactor, and reactor is to make with the commodity cover plate materials repacking of building the greenhouse.The thickness of slab 1.6cm of reactor meets the long-pending 2.2m2 of being of optical surface, and the liquid amount of reactor is between 25-27L, and specific surface area is 80m-1.Tredici vertically places this cover plate materials, and adopts the gas lift circulation to mix, and algae liquid flows along vertical direction, thus be called vertical caulking groove plate-type reactor (Vertical AlveolarPanel, VAP).
4. multilayer board-like bioreactor (the Pulz O that is arranged in parallel, Gerbsch N, Bachholz R.Lightenergy supply in plate-type and light diffusing optical fiber bioreactors[J] .Journal ofApplied Phycology, 1995,7:145-149): the Pulz etc. of Germany has reported the multilayer board-like bioreactor research that is arranged in parallel.The specific surface area of this reactor is 60m-1.The most for a long time, reactor is made up of 21 parallel plate-type reactor unit in the test, and the thickness of veneer is 2.5cm, and cumulative volume reaches 6000L, wherein dark volume 680L.The light transmitting surface of this plate-type reactor is long-pending to be 436m2, the about 100m2 of floor space.Some direct projection of sunlight is to the reactor sheet face, and major part enters reactor by reflection or scattering.
5. the board-like bioreactor of inclination bubbling (Hu Qiang, Richmond A.Productivity andphotosynthetic efficiency of Spirulina platensis as affected by light intensity, algaldensity and rate of mixing in a flat plate photobioreactor[J] .Journal of AppliedPhycology, 1996,8:139-145): Hu etc. have reported the research of this board-like bioreactor.The plate case of this reactor is made of glass.The reactor box house does not have vertical or horizontal dividing plate, forms the complete and continuous internal space, mixes in the bubbling mode.The high 70cm of this board-like bioreactor, wide 90cm.
6.Graziella Deng having designed a kind of reactor of forming by six flat units (Modular Flat PanelPhotobioreactor, MFPP) (Graziella Chini Zittelli et al.A modular flat panelphotobioreactor (MFPP) for indoor mass cultivation of Nannochloropsis sp.Underartificial illumination[J] .Journal of Applied Phycology, 2000,12:521-526), and studied the influence of intensity of illumination to becoming to be grouped in the algae.Each flat unit dress liquid of this reactor is long-pending to be 20.5L, and light-receiving area is 3.4m2, and fluorescent tube is placed between each flat unit, forms a sealed whole body, and whole reactor is placed in the cell of a temperature controllable.
7.J a kind of novel flat bioreactor (J rg Degen of designs such as rg Degen with baffle plate, Andrea Uebele et al.A novel airlift photobioreactor with baffles for improved lightutilization through the flashing light effect[J] .Joumal of Biotechnol., 2001,92:89-94).This reactor is made by resin glass, and illuminating area is 0.084m2, takes two kinds of light paths (15mm and 30mm), and volume is respectively 1.5L and 3.0L, wherein baffle plate 7% the volume that accounts for.The passage that flows downward in the circulation of algae liquid is positioned at a side of reactor width, (this space is less), rising passway is divided into 6 parts that communicate by 5 horizontal baffles, and these 5 baffle plate alternative are fixed on the front and back panel of reactor, to increase turbulence, promote algae liquid to mix.
The external disclosed patent of invention about board-like bioreactor mainly contains:
1.Jacques the bioreactor of Delente etc. (United States Patent (USP) .US005104803A, 1992-04-14).This reactor is box reactor, can insert many rows and the isolated fluorescent tube of liquid in the reactor as inner light source usefulness, at reactor bottom, a gas distribution gas air feed is arranged between per two bank light pipes, and liquid is circulated around fluorescent tube.
2.Scot the box bioreactor (United States Patent (USP) of the inner light source of D.Hoeksema etc.US?005162051A，1992-11-10)。Four row's inner light sources are arranged in this reactor cavity, this four row's inner light source and nutrient solution are isolated, and form independently cavity, simultaneously also as the baffle plate of separating between algae liquid rising passway and decline passway, in the appropriate position ventilation of reactor bottom, nutrient solution flows around baffle plate and forms a plurality of circulations.So both improved the utilization ratio of light, overcome frustule again, improved the culture efficiency of little algae easily attached to the problem on the inner light source.
3.Tredici Deng flat bioreactor (italian patent .IT 1241751,1994-02-01).A plurality of baffle plates are arranged in this reactor,, nutrient solution is flowed around baffle plate form a plurality of circulations in the appropriate position ventilation of reactor bottom.
4.James the box bioreactor of inner light source of S Craigie etc. (United States Patent (USP) .US 2003/0059932A1,2003-03-27).This reactor has adopted the form of inner light source, and the cavity that inner light source independently is installed is arranged, and has overcome frustule easily attached to the problem on the inner light source.
5.Boussiba the flat bioreactor of Sammy etc. (world patent .WO 2005/006838A2,2005-01-27).This reactor is made up of rectangular metal network, plastics bag, gas circuit and temperature-controlled tube etc.During use, plastics bag is installed in the network, again nutrient solution and little algae is made an addition in the plastics bag, by bottom inflow nutrient solution is formed and circulate, carry out temperature control by serpentine tube.This structure of reactor is simple, and is with low cost, is applicable to scale operation.
In above-mentioned patent, the box bioreactor of invention such as Jacques Delente is though used internal light source, improved the utilization ratio of light, but it can not accept the exterior light photograph, has increased operating cost, and also there is the problem of heat radiation difficulty in Embedded inner light source; The box bioreactor of inner light source of inventions such as Scot D.Hoeksema is based on the invention of Jacques Delente, though improved the installation method of inner light source, but the same problem that has the heat radiation difficulty, and as box reactor, utilization to external light source is also very limited, though its cumulative volume reaches 0.3m in addition
3About, but actual liquid amount only is 0.11-0.13m
3, this has increased manufacturing cost greatly; The flat bioreactor of inventions such as Tredici etc. and Boussiba Sammy adopted the exterior light photograph fully, thereby the utilization ratio of light is low; The box bioreactor of inner light source of invention such as James S Craigie can not utilize external light source, and have heat dissipation problem equally.
In all little algae culturing process, illumination is the most critical factor that little algae high-density culture of influence and restriction bioreactor amplify.The main design thought of bioreactor is to make microalgae cell can obtain sufficient illumination.Though the flat photo-bioreactor system of above-mentioned short light path has improved illumination condition, too short light path has increased the difficulty of amplifying, and the amplification of reactor assembly can only realize that this has just increased the input of cost by increasing reactor unit.And for the bigger reactor of light path, though after using inner light source, improved the utilization ratio of light, but the arrangement mode of its inner light source can not effectively solve the problem that little algae is attached to inner light source lamp cover, and Fu Za inner light source arrangement mode also can increase resistance in addition, influences the circulation of algae liquid; And except the novel flat bioreactor of designs such as J rg Degen, all do not have to consider how effectively to strengthen the mixing of algae liquid at direction of illumination, limited the utilization ratio of light, increased the difficulty of amplifying.The reactor of designs such as J rg Degen has increased liquid flowing resistance owing to added the polylith plate washer in inside, and energy consumption is increased, and also is unfavorable for cleaning and amplifies owing to the internal structure complexity simultaneously.All realize that by increasing reactor unit maximum reactor unit volume is less than 300L for fairly large culture systems, this has increased manufacturing cost greatly.
Therefore, amplification for flat photo-bioreactor system, should be when increasing light path raising reactor unit volume, specific surface area by the augmenting response device, increase source of artificial light and promote algae liquid to wait the utilization ratio that improves luminous energy, thereby satisfy the needs of little algae high-density culture in the mixing of direction of illumination.
At present, rarely has report to the research of flat bioreactor is domestic, there is not the invention disclosed patent yet, disclosed other form bioreactor patent of invention mainly contains: Guo offers sacriffices to the gods or the spirits of the dead far, Li Zhiyong, the pipeline gas lift-type magnetic treatment bioreactor both culturing microalgae device (Chinese patent .CN1187535,1998-07-15 authorizes) of Li Lin etc.; The spirulina light illuminating tower-tray optic bio-reactor culturing system of Xu Mingfang and control method thereof (Chinese patent .CN1201826,1998-12-16); Liu Xin, Yuan Jianping, the bioreactor of Wang Jianghai etc. promote Haematocoocus Pluvialls propagation to obtain the method (Chinese patent .CN1392243,2003-01-22 authorizes) of pure strain; The automatic continuous production of track optical biological reactor of Lin Zhenneng (Chinese patent .CN1394951,2003-02-05 authorizes); Cong Wei, Kang Ruijuan, airlift photobioreactor of the integral type of Cai Zhaoling and uses thereof (Chinese patent .CN1472305,2004-02-04); Luo Qijun, Pei Luqing, Yan Xiaojun etc. a kind of cultivate the method for little algae and the photo-bioreactor system of use (Chinese patent .CN1563346,2005-01-12); Feng Bin, Bi Yonghong, the airlift photobioreactor of a kind of Haematocoocus Pluvialls high-density culture of Jiang Juan etc. (Chinese patent .CN1680539,2005-10-12); Liu Jianguo, Liu Wei, the optical-biological reaction apparatus that little algae of Wang Zengfu etc. is cultivated (Chinese patent .CN1718720,2006-01-11); Liu Jianguo, Wang Zengfu, the pipeline bioreactor that the microalgae mass of Liu Wei etc. is cultivated (Chinese patent .CN1721523,2006-01-18).
Above patent all is air lift type tubulose or column bioreactor, though tubular reactor is easy to amplify, it is poor that oxygen is resolved, and cleans difficulty, and power consumption is big; The long light path of cylindrical reactor has then increased the difficulty of amplifying.In order to increase mixing, the bioreactor of Liu Xin etc. has adopted speed governing paddle wheel agitator and volume water wheels device, the pipeline bioreactor of Liu Jianguo etc. has adopted mechanism to promote flowing of water body, these devices have not only increased energy consumption, and the shearing force of generation also can damage frustule.In order to improve total light intensity of reactor, remedy the deficiency of natural lighting, source of artificial light has been widely used in the cultivation of little algae in the photoreactor.External and the built-in dual mode of the normal employing of source of artificial light at present, in the illumination system of above-mentioned patent, the track optical biological reactor of Lin Zhenneng places illumination system in " well " font solid space that intersects stack communicating pipe and form, the airlift photobioreactor of Feng Bin etc. has adopted inner light source and outer light source simultaneously, and other reactor assembly has all adopted the form of outer light source.External light source is easy to operate, but optical energy utilization efficiency is low; Built-in light source has improved the utilization ratio of light significantly, but because the attached wall of the heat of its generation and cell has limited the application of built-in light source.
Therefore, this area still needs the dull and stereotyped bioreactor that is applicable to large-scale culturing micro-algae that provides new.
Summary of the invention
The present invention is guidance with advanced person's bioreactor design concept, the angle of optical energy utilization efficiency from improve reactor has emphatically studied and has designed that a kind of specific surface area is big, the efficiency of light energy utilization is high, algae liquid mixes and the good novel little algae production system of circulatory condition.
Particularly, the invention provides a kind of dull and stereotyped bioreactor, described dull and stereotyped bioreactor comprises:
Transparent flat casing;
At described box house a plurality of hollow flow apron cavitys are arranged, described flow apron inside cavity is equipped with one or more source of artificial light, and described flow apron cavity is installed on the interior vertical plane of described reactor, has between each flow apron cavity at interval; With
The device of supply gas.
In a preferable embodiment, be spaced apart 10-100mm between described each flow apron cavity.
In another preferable embodiment, the cumulative volume of described dull and stereotyped bioreactor is 1000L or bigger.
In another preferable embodiment, the device of described supply gas is the gas distributor at described reactor bottom.
In another preferable embodiment, described flow apron cavity is installed on the interior same vertical plane of described reactor, this vertical plane is divided into fluid rising passway and fluid decline passway with the inside reactor zone, and the device of described supply gas is in described fluid rising passway.Preferable, the ratio of the sectional area of described fluid rising passway and fluid decline passway is 0.5: 1 to 3: 1.
In another preferable embodiment, described flow apron cavity is installed on the interior a plurality of vertical planes of described reactor, this vertical plane is divided into a plurality of fluid rising passwaies and fluid decline passway with the inside reactor zone, and the device of described supply gas is in described fluid rising passway one or more.
In another preferable embodiment, described dull and stereotyped bioreactor also comprises the device of controlled temperature, the device that detects pH, the device that detects dissolved oxygen, device and the charging and the discharge device of detected temperatures.
In another preferable embodiment, the source of artificial light in the described flow apron cavity links to each other with the light path control system.
The present invention also relates to the application of above-mentioned dull and stereotyped bioreactor in cultivating little algae on the other hand.
The present invention has effectively overcome the problem that traditional bioreactor microalgae culture system occurs in amplification process, its advantage and novelty mainly show following 5 aspects:
1) employing of inside reactor more piece flow apron hollow cavity has not only improved the overall mixing of reactor, and to have strengthened fluid radially be the mixing of direction of illumination, more effectively promotes liquid circulation, reduces power consumption, has improved utilization of incident light.
2) be provided with inside reactor more piece baffle plate hollow cavity innovatively, place source of artificial light therein, avoided the shortcoming of external and simple built-in light source, the optical energy utilization efficiency height.
3) the light application ratio surface-area is big, and surfaces externally and internally can be subjected to light simultaneously, has increased the specific surface area of illumination, has reduced light path simultaneously.
4) reactor volume big (1000L), and the geometrical shape of its design is easy to further amplification.
5) be easy to processing and fabricating, easy to operate, processing and use cost are low.
The objective of the invention is to improve the illumination utilising efficiency of bioreactor from above several respects, simultaneously the reactor culture systems is optimized, to obtain to be fit to the novel microalgae culture system of little algae large-scale low-cost high-efficient culture, accelerate the practical application of bioreactor in little algae batch production is produced, promote the development of little algae culture technique industrialization.
Description of drawings
Fig. 1 has shown when inner light source is divided into three joints, the front view of the more piece-air lift type-inner light source-dull and stereotyped bioreactor of side ventilation, and wherein each symbol implication is as follows: 1 dull and stereotyped reactor casing; 2 inlet pipe; 3 flow apron hollow cavities; 4 internal light sources (energy-saving daylight lamp); 5 gas distributors; 6 temperature control serpentine tubes; 7 light path control systems; 8 hand holes (charge cavity, inoculation hole); 9 venting holes; 10 top pressure closures; 11pH, dissolved oxygen, temperature sensor probe; 12 discharge gates; 13 fluid rising passwaies; 14 fluid decline passwaies; Gap between 15 flow apron cavitys.
Fig. 2 has shown when inner light source shown in Figure 1 is divided into three joints, the side-view of the more piece-air lift type-inner light source-dull and stereotyped bioreactor of side ventilation.
Fig. 3 has shown when inner light source is divided into three joints, the side-view of the more piece-air lift type-inner light source-dull and stereotyped bioreactor of middle ventilation, wherein each symbol implication such as Fig. 1.
Fig. 4 has shown Isochrysis galbana 3011 culture effect comparison diagrams in 1000L more piece-air lift type-inner light source under the natural lighting-dull and stereotyped bioreactor and the dull and stereotyped bioreactor of single-unit.
Fig. 5 has shown different culture systems intermediate cam brown algae culture effect comparison diagrams.
Fig. 6 has shown under the natural lighting Isochrysis galbana 3011 culture effect comparison diagrams in the different culture systems.
Fig. 7 has shown that natural lighting adds under the artificial light condition Isochrysis galbana 3011 culture effect comparison diagrams in the different culture systems.
Embodiment
As previously mentioned, the geometrical shape of bioreactor is one of key factor that influences optical energy utilization efficiency.Flat bioreactor is for the reactor of other form, have simultaneously that the light application ratio surface-area is big, illumination utilization ratio height, mix, oxygen resolve fast, amplify advantages such as relatively easy, therefore have certain advantage as foundation structure with flat-plate reactor.But most of flat bioreactor light paths are too short at present, and scale is less, adopts external light source, and the efficiency of light energy utilization is low, and the mixing that mixes situation, particularly direction of illumination simultaneously also remains to be improved.The present invention promptly is to be foundation structure with flat bioreactor, and it is carried out improving and optimizating of novelty, makes it be adapted to the extensive high-efficient culture of little algae.
The mixing effect of fluid of inside reactor is to influence key factor of illumination utilising efficiency in the reactor.The fluid of realization response device inside mixes and can realize by mechanical stirring and ventilation.Because the attached wall of little algae and the susceptibility of convection cell shearing force, there is churned mechanically reactor to be unfavorable for cleaning and is unfavorable for cultivating algae, and cost and maintenance cost height the shearing force sensitivity, be not suitable for the economic production of little algae; Driving the circulation of algae liquid by ventilation is the most frequently used method during present little algae is cultivated, ventilation simultaneously can provide micro algae growth required carbon source again, but the algae liquid that ventilating mode commonly used drives circulation is slower, is difficult to reach sufficient mixing, and especially the mixing on direction of illumination is very poor.
The present invention is promptly by taking particular structure at inside reactor, promoted on the one hand algae liquid the mixing of direction of illumination with totally mix the speed of circulation of raising algae liquid, thereby the utilization ratio of raising light.On the other hand, the present invention organically combines the advantage of inside and outside light source, has avoided shortcoming separately, when improving the utilization ratio of little algae to light, has solved frustule easily attached to this problem on the inner light source, and has improved the heat dissipation problem of inner light source.
Specifically, the invention provides a kind of dull and stereotyped bioreactor, described dull and stereotyped bioreactor comprises: transparent flat casing; At described box house a plurality of hollow flow apron cavitys are arranged, described flow apron inside cavity is equipped with one or more source of artificial light, and described flow apron cavity is installed on the interior vertical plane of described reactor, has between each flow apron cavity at interval; Device with supply gas.More piece-air lift type of the present invention-inner light source-dull and stereotyped bioreactor is applicable to cultivates various little algaes.The present invention there is no too much restriction for concrete cultured microalgae, and algae usually can be by commercially available approach acquisition slightly for this.
To specify implementation method of the present invention from aspects such as the light application ratio surface-area of increase reactor, the illumination system that adopts uniqueness, reinforcement algae liquid internal mix and circulation, increase photoreactor volume, detection control and fitting operations below.
1. increase the light application ratio surface-area of reactor
The present invention adopts transparent rectangular flat plate formula casing, in order to utilize natural lighting fully, cuts down the consumption of energy, and has increased external surface area in design as much as possible, is example with the dull and stereotyped bioreactor of 1000L, and the area that can accept natural lighting is 6.5m
2Simultaneously transparent flow apron cavity wall is as inside reactor illumination surface-area, and the illuminating area that can receive source of artificial light and part natural light is 4.2m
2This outside and the inner design that can receive illumination have simultaneously increased the illuminating area of reactor greatly, and its light application ratio surface-area reaches 11: 1, considerably beyond the light application ratio surface-area of other flat board or cylinder shape bioreactor.
2. adopt unique source of artificial light modes of emplacement
Reactor has knotty flow apron hollow cavity, and two rows totally six small racks are installed on the cavity inner wall, is used to place energy-saving fluorescent lamp tube, and its light intensity can be regulated by the light path control system.Therefore, the flow apron cavity is except the guide functions with general airlift reactor, also provide independently space for built-in source of artificial light, avoided the frond to contact, thereby solved the problem that built-in light source adheres to surface of the light tube, shuts out the light owing to the phototaxis of little algae with the direct of light source.Simultaneously source of artificial light is positioned over internal cavities and can avoids the low problem of external source of artificial light illumination utilising efficiency again, such modes of emplacement had both improved the illumination utilising efficiency, convenient and safe again operation.In addition, in order to improve the utilising efficiency of source of artificial light, that has taked that artificial lighting and natural lighting organically combine gives light technology, promptly natural light intensity be lower than certain value (as at dusk and overcast and rainy) time open source of artificial light again.
3. the integral body of algae liquid circulation in the reinforcement reactor, mix and the mixing of direction of illumination up and down
Inside reactor has vertical flow apron hollow cavity, reactor is divided into two different fluid channels of cross-sectional area from thickness direction, wider passages is placed gas distributor near the place, bottom, this passage is as the rising passway of air lift type structure, and a narrower side is as the decline passway of air lift type structure, gas distributor and bottom and liquid level and water conservancy diversion plate washer cavity all keep suitable distance, improve reactor mixing from top to bottom so greatly, promoted the integral body circulation of algae liquid.
The flow apron hollow cavity is cut apart in vertical direction by a certain percentage simultaneously, form the flow apron cavity configuration of multi-section type, and keep certain clearance between more piece flow apron cavity, allow reactor rising passway and decline passway, part algae liquid is refluxed at intermediate connection.In the common airlift reactor, the liquid stream exchange that the circulation of liquid stream only just has direction of illumination in the top or the bottom of reactor, and because the decay of illumination in algae liquid causes light intensity skewness on the direction of illumination, this obtains illumination to the inside reactor frustule is disadvantageous.Multi-section type flow apron cavity configuration can strengthen the mixing of direction of illumination, shortens the mean circulation time (MCT) of frustule between rising passway and decline passway, and this is considerable to production-scale large volume bioreactor.
In traditional bioreactor, promote reactor direction of illumination blended method for to add various baffle plates at inside reactor, though this has promoted the mixing of direction of illumination to a certain extent, the inside reactor complex structure, processing is all very difficult with cleaning; And add baffle plate and increased loss of pressure head, be unfavorable for the integral body circulation of algae liquid and mix up and down that power consumption is increased, and production cost improves, and is unfavorable for the amplification of reactor.
The present invention is by adopting the form of special more piece-air lift type, avoided the shortcoming of traditional reactor, strengthen the integral body circulation of algae liquid in the reactor simultaneously, mix and the mixing of direction of illumination up and down, strengthened the overall mixed effect of reactor greatly, thereby improved the illumination utilising efficiency.The fluid-flow rate of inside reactor can be controlled by gas flow and gas distributor simultaneously.
4. the volume of reactor
Realize the low-coat scale cultivation of little algae in the bioreactor, one of crucial is exactly that the volume of reactor wants enough big, and this is the bottleneck that limits bioreactor practical application in little algae batch production production at present.The present invention is on the basis of for many years structure of reactor being optimized, the flat bioreactor of the 1000L that develops, bioreactor greater than most of domestic other forms, and research and development the time fully take into account the feasibility that reactor further amplifies, and what (as: the sectional area maintenance ratio of fluid rising passway, fluid decline passway and baffle plate cavity is 2: 1: 1) carried out helping amplifying on the ratio of structure of reactor and each parts is reasonably combined.
5. detect control and fitting operation
Reactor of the present invention is furnished with the on-line detecting system of pH, dissolved oxygen and temperature, and can realize the secondary control of computer.Carry out the reasonable adjusting of culture process according to the real-time detected result of these parameters, be embodied in: carry out the adjusting of air flow according to the height of pH, when the pH of algae liquid surpasses growth scope, can increase air flow to keep pH in suitable scope; Height according to dissolved oxygen is judged the photosynthetic power of frustule, and carries out rational illumination in view of the above and regulate; Be useful on temperature controlled serpentine tube in the reactor, the temperature that enters the temperature control water of serpentine tube by adjusting is controlled culture temperature.
Whole reactor is vertically mounted on the support of triangular form, play the effect of stablizing and adding gas-solids reactors, there is a horizontal support at the middle part on two sides before and after reactor simultaneously, can prevent along with reactor volume increase and hydraulic pressure increase caused reactor wall bulging deformation problem.
Reactor is easy to operate, and its inlet channel directly is connected with reactor, and the valve of opening conduits can add in the trend reactor cultivates water or water for cleaning; Drain hole can be connected with other system by pipeline simultaneously, as being connected with nursery pond in sea farming, realizes throwing something and feeding automatically of bait.
Further specify the structure of more piece-air lift type of the present invention-inner light source-dull and stereotyped bioreactor below in conjunction with accompanying drawing.
Fig. 1 has shown the front view of more piece-air lift type of the present invention-inner light source-dull and stereotyped bioreactor, and Fig. 2 is the side-view of this flat board bioreactor.Among the figure, the arrow in the reactor is represented the direction that circulates of algae liquid.For guaranteeing the illumination utilization ratio, application such as each parts in the dull and stereotyped bioreactor of the present invention such as casing, hollow flow apron cavity are made the light material transparent.Simultaneously, in order to satisfy the requirement of inside reactor blended, described material also needs to have certain intensity.Described material can be selected from synthetic glass or polymer materials, and preferable material is a synthetic glass.
As depicted in figs. 1 and 2, the shell of more piece-air lift type of the present invention-inner light source-dull and stereotyped bioreactor is flat casing.Term " flat board " has known to those skilled in the art and the implication of approval, and the width (or thickness) of its expression casing is far smaller than the height and the length of casing.Therefore, when the width of casing between 300-800mm, length and the height reactor of (its concrete size can according to real needs and structure design and different) between 1000-2000mm also belongs to flat-plate reactor.For example, under situation of placing the flow apron hollow cavity in two vertical planes shown in Figure 3, the width of casing can be big slightly.
In the transparent casing 1 of reactor, inlet pipe 2 is connected by the BULKHEAD UNION of top by top pressure closure 10 and enters reactor, and the lower end links to each other with the device 5 of supply gas.The device 5 of described supply gas can adopt those devices well known to those skilled in the art, as gas distributor.In order to ensure mixing preferably and the air feed effect, gas distributor 5 should be in the bottom of reactor.Institute's gas supplied can be cultivated needs according to frond and be decided.For example, gases used can be air, also can be to contain finite concentration CO
2Mixed gas.The gas that enters reactor is not only for the frond growth provides necessary carbon source, and based on the air lift type principle, the integral body that can drive algae liquid circulates.
Transparent casing internal fixing a plurality of flow apron hollow cavities 3, rectangular casing is divided into fluid rising passway 13, fluid decline passway 14 and flow apron hollow cavity three parts.In order to ensure with promote that the mixing in the reactor is abundant, flow apron hollow cavity 3 can be gone up the different shape that lower rim can be designed to be able to reduce fluid resistance.Have one or more little supports in the described flow apron hollow cavity 3, thereby can place one or more source of artificial light 4 as required.Source of artificial light 4 can be taked light source commonly used in the photo bio cultivation field, and wherein energy-saving daylight lamp is preferable.But those skilled in the art also can adopt the mode of placing a plurality of pointolites on the length direction of transparent casing 1 fully.The light intensity of source of artificial light 4 can be regulated and control by the light path control system 7 of outside.Described flow apron hollow cavity can be on the sidewall of the one or both ends width that is fixed on the transparent plate casing, so that source of artificial light 4 is installed, and be connected with the light path control system 7 of outside.
In a preferable embodiment, as depicted in figs. 1 and 2, described a plurality of flow apron hollow cavity 3 is distributed in same or a plurality of vertical planes of width, and a plurality of source of artificial light 4 in each flow apron hollow cavity 3 also are distributed in same or a plurality of vertical planes of width.Yet, it will be appreciated by those skilled in the art that this has only represented a preferable embodiment of the present invention, and scope of the present invention and should not be limited to described concrete arrangement mode shown in the drawings.Even believe in the not strict same vertical plane that is distributed in width of each flow apron hollow cavity 3 or each source of artificial light 4 and also should be able to realize goal of the invention of the present invention.
Dull and stereotyped bioreactor of the present invention is knotty, that is, each flow apron hollow cavity 3 separates, have certain clearance 15 therebetween, this gap 15 is usually between 10-100mm, and is preferable between 20-80mm, better between 30-70mm, also want good being about about 50mm.Shown in the horizontal arrow among Fig. 2, described gap 15 makes fluid rising passway 13 and fluid decline passway 14 at the intermediate connection of reactor, thereby makes part algae liquid reflux, and has promoted mixing.For example, two, three, four, five or more a plurality of flow apron hollow cavity 3 that separates can be arranged in vertical plane, thereby form two joints, three joints, four joints, five joint or more knotty dull and stereotyped bioreactors.The flow apron hollow cavity 3 of top and bottommost should maintain a certain distance (about usually 100-200mm) with the top and the bottom of casing, so that the circulation of algae liquid mixes.
As mentioned above, flow apron hollow cavity 3 is divided into fluid rising passway 13, fluid decline passway 14 and flow apron hollow cavity three parts with rectangular casing.Gas distributor 5 is distributed in gas equably and cultivates in the algae liquid, driving algae liquid then circulates to fluid decline passway 14 from fluid rising passway 13, simultaneously, part algae liquid by the gap 15 of 3 of flow apron cavitys from decline passway to the rising passway transverse flow, thereby the fluid of strengthening direction of illumination mixes, and utilizes luminous energy better.In a preferable embodiment, the sectional area of described fluid rising passway 13 (on width) should be greater than the sectional area of fluid decline passway 14, for example, for the 1.5-3 of the sectional area of fluid decline passway 14 doubly, preferable be 2-2.5 times.5 of gas distributors are positioned at fluid rising passway 13.
As shown in Figure 1, the temperature regulating device (as the temperature control serpentine tube) 6 of control culture temperature is installed also in the reactor, the temperature that enters the temperature control water of serpentine tube by adjusting is controlled culture temperature.Light path control system 7 is used to regulate the time and the light intensity magnitude of artificial lighting.In addition, reactor also has and is used for hand hole 8 (culturing process is added a cover and sealed), venting hole 9, reactor top pressure closure 10 reinforced and inoculation; And the transmitter 11 that is used to measure algae liquid temp, pH and dissolved oxygen in the reactor, so that the process data of obtaining is delivered to the control analysis terminal, be used for the optimal control and the result treatment of culture condition.
Accompanying drawing 3 has shown another embodiment of more piece-air lift type of the present invention-inner light source-dull and stereotyped bioreactor.In this embodiment, a plurality of flow apron hollow cavities 3 that distributed in two vertical planes on the width in transparent casing 1, thus be that fluid rising passway 13, both sides are the algae liquid circulation pattern of fluid decline passway 14 in the middle of having formed.At this moment, should make gas distributor 5 be positioned at fluid rising passway 13.Certainly, those skilled in the art also can expect the arrangement mode that adopts after having read above-mentioned two specific embodiments of the present invention, as distribution flow apron hollow cavity 3 in the three or more vertical planes on the width in transparent casing 1, and arrange one or more gas distributors 5 as required.
In specific embodiments of the invention, used more piece-air lift type-inner light source-dull and stereotyped bioreactor cumulative volume is 1000L, its basic physical dimension is 1800 * 1450 * 400 (height * length * wide mm of unit), the rising passway width is 200mm, and decline passway thickness and flow deflector hollow cavity width are all 100mm.Gap between each flow apron cavity is 50mm, and first flow apron cavity upper edge is 200mm to the distance of reactor head, and the 3rd flow apron cavity lower edge is 120mm to the distance of reactor bottom.Yet, it will be appreciated by those skilled in the art that more piece-air lift type of the present invention-inner light source-dull and stereotyped bioreactor is not limited only to this concrete mode.
In order to describe using method of the present invention and advantage place thereof in detail, be specifically described with three embodiments below:
Embodiment 1: adopt the comparison of the dull and stereotyped bioreactor culture efficiency of the present invention and single-unit
Under natural lighting, compared the culture efficiency of the dull and stereotyped bioreactor of 1000L more piece-air lift type of the present invention-inner light source-dull and stereotyped bioreactor and 1000L single-unit air lift type.Used substratum is: NaNO
3: 90mg/L, KH
2PO
4: 5mg/L, FeC
6H
5O
75H
2O:0.5mg/L; Culture temperature is 25～30 ℃; 1000L more piece flat-plate reactor and the actual dress of 1000L single-unit flat-plate reactor liquid are long-pending for about 800L, and vapour-liquid ratio is 0.1vvm; Cultivating used algae kind is Isochrysis galbana 3011 (can commercially availablely buy).
After test is passed through preliminary treatments such as precipitation, the husky filter of pressurization with seawater, adopt the oxidation style sterilization again, every cubic metre of seawater adds the commercially available sodium hypochlorite solution of 1L (available chlorine content about 10%) approximately before using, the ventilation mixing, the amount of handling after 8～10 hours by every L sodium hypochlorite solution 100 grams adds the Sulfothiorine neutralization, and with the potassium iodide and starch reagent test whether exist (be that Sulfothiorine is excessive slightly this moment usually) of chlorine residue is arranged, as no chlorine residue, then continue ventilation 1～2 hour, the Sulfothiorine that oxidation is excessive slightly.
Under the natural lighting condition, Isochrysis galbana 3011 cultivation results of dull and stereotyped reactor of 1000L more piece and the dull and stereotyped reactor of 1000L single-unit as shown in Figure 4.Cultivate early stage (before the 46h), frustule growth difference is little in two reactors; After cultivating 46h, algae cell density is higher than the single-unit flat-plate reactor gradually in the more piece flat-plate reactor; Be cultured to 118h, reaching high-cell density in the more piece flat-plate reactor is 755 * 10
4Individual/ml, and the single-unit flat-plate reactor is 662 * 10
4Individual/ml, the former improves 0.14 times than the latter.The special more piece diaphragm structure of this explanation more piece-air lift type-inner light source-dull and stereotyped bioreactor has been strengthened the mixing of algae liquid, the particularly mixing of direction of illumination, promoted the overall circulation of algae liquid, make the frustule in the reactor can both obtain rational illumination, improve the utilising efficiency of luminous energy, more helped the growth of frustule.
Embodiment 2: the Phaeodactylum tricornutum that adopts high-density culture of the present invention widespread use in seawater seed rearing
Used substratum is: NaNO
3: 90mg/L, KH
2PO
4: 5mg/L, FeC
6H
5O
75H
2O:0.5mg/L; Culture temperature is 20～25 ℃; The actual dress of 1000L flat-plate reactor liquid is long-pending for about 800L.Test with method of seawater treatment with embodiment 1.
Respectively under the situation of natural lighting and increase source of artificial light, the culture efficiency that has compared 1000L flat-plate reactor of the present invention and 300L cylindrical reactor, the 300L cylindrical reactor is a kind of upright bag of peripheral belt supporting frame, is a kind of advanced culture systems that present domestic batch production bait micro-algae is cultivated usefulness.In the culturing process, the natural light intensity average out to of various reactor surface: the about 8000lx of sunny side, the about 3500lx of the back; Fluorescent tube-the liquid volume ratio is 6 fluorescent tube/300L to increase the source of artificial light group, and (18:00-7:00) carries out irradiation at night, and wherein 1000L flat-plate reactor source of artificial light form is an inner light source, and the 300L cylindrical reactor is outer light source.Vapour-liquid ratio in each reactor is 0.1vvm, and experimental result as shown in Figure 5.
Under identical vapour-liquid ratio, night, the dull and stereotyped reactor growth situation of 1000L was significantly better than the 300L cylindrical reactor when not increasing source of artificial light.At the preceding 96h that cultivates, when cell concn is low, two kinds of reactor inner cell growth velocity basically identicals; But along with the increase of cell density, the growth of cell is subjected to the light restriction in the 300L cylindrical reactor, and it is slack-off to grow, and cell count begins to occur descending during to 146h; And because the light application ratio surface-area of flat-plate reactor is big, illumination condition is good, so along with the increase of cell density, cell still keeps quick growth.The highest algae cell density arrives 888 * 10 in the dull and stereotyped reactor of final 1000L
4Cell/ml, and the highest algae cell density is 633 * 10 in the 300L cylindrical reactor
4Cell/ml; Cell density is enhanced than cylindrical reactor in the dull and stereotyped reactor, and density has improved 0.4 times when cultivating end.The structure that more piece-air lift type-inner light source-dull and stereotyped bioreactor is described thus is greatly improved the utilising efficiency of light.
Under identical vapour-liquid ratio, night, the cell growth was obviously accelerated in two types the reactor when increasing source of artificial light, illustrated when not increasing source of artificial light in two kinds of reactors that illumination remains the bigger limiting factor of cell growth.When two kinds of reactors increase the source of artificial light of identical fluorescent tube-liquid volume ratio simultaneously, when early stage, algae cell density was low, both growth velocitys are suitable substantially, but the later stage is along with the increase of cell density, cylindrical reactor is because the situation of mixing is poor, the illumination utilising efficiency is low, and the light restriction causes cell to stop growing substantially.The highest algae cell density arrives 1472 * 10 in the dull and stereotyped reactor of final 1000L
4Cell/ml, and the highest algae cell density is 997 * 10 in the 300L cylindrical reactor
4Cell/ml; Cell density is enhanced than cylindrical reactor in the dull and stereotyped reactor, and density has improved nearly 0.5 times when cultivating end.This structure that further specifies more piece-air lift type-inner light source-dull and stereotyped bioreactor is greatly improved the utilising efficiency of light.
By above two groups of experimental results as can be seen, because more piece-air lift type-inner light source-dull and stereotyped bioreactor light path is short, and adopted special more piece-air lift type form, promoted of the mixing of algae liquid greatly at direction of illumination, make each frustule can both obtain illumination fully, thereby improved the utilization ratio of illumination, promoted the growth of frustule.Simultaneously, can find that when not increasing source of artificial light, the whole density of cell has improved 0.4 times than cylindrical reactor in the dull and stereotyped reactor by comparing two groups of experiments; When increasing source of artificial light, the whole density of the former cell has improved nearly 0.5 times than the latter, and this source of artificial light modes of emplacement (internal light source) that dull and stereotyped reactor is described has obviously improved the illumination utilising efficiency.
The volume of more piece-air lift type-inner light source-dull and stereotyped bioreactor is three times of cylinder shape, so both realized the successful amplification of reactor, improved culture efficiency again, more piece-air lift type form and internal light source modes of emplacement special in this explanation more piece-air lift type-inner light source-dull and stereotyped bioreactor have promoted that direction of illumination mixes in the reactor, improve the efficiency of light energy utilization, more helped the growth of frustule.
Embodiment 3: the Isochrysis galbana 3011 that adopts high-density culture of the present invention widespread use in seawater seed rearing
Used substratum is: NaNO
3: 90mg/L, KH
2PO
4: 5mg/L, FeC
6H
5O
75H
2O:0.5mg/L; Culture temperature is 24～32 ℃; The actual dress of 1000L flat-plate reactor liquid is long-pending for about 800L.Test with method of seawater treatment with embodiment 1.
In natural lighting with add under the situation of source of artificial light, compared the culture efficiency of 1000L more piece-air lift type of the present invention-inner light source-dull and stereotyped bioreactor and 300L cylindrical reactor respectively.Increase in the source of artificial light group, add source of artificial light during (8:00-18:00) daylight light intensity＜8000lx when daytime, fluorescent tube-liquid volume ratio is 6 fluorescent tube/300L, wherein 1000L flat-plate reactor source of artificial light form is an inner light source, the 300L cylindrical reactor is outer light source, and the vapour-liquid ratio in each reactor is 0.1vvm.
Under the natural lighting condition, Isochrysis galbana 3011 cultivation results of dull and stereotyped reactor of 1000L and 300L cylindrical reactor as shown in Figure 6.Cell growth curve in the dull and stereotyped reactor is from 100 * 10
4Beginning is higher than cylindrical reactor the same terms under gradually about individual/ml, and cultivation 120h reaches high-cell density, and dull and stereotyped reactor is 453 * 10
4Individual/ml, cylindrical reactor is 377 * 10
4Individual/ml, the former improves 0.2 times than the latter.
Add on natural lighting and daytime under the artificial lighting condition, Isochrysis galbana 3011 cultivation results as shown in Figure 7 in dull and stereotyped reactor of 1000L and the 300L cylindrical reactor.Dull and stereotyped reactor inner cell growth curve is from 70 * 10
4Be higher than the cylindrical reactor under the similarity condition behind the cells/ml always, cultivate and to reach high-cell density 362 * 10 in the dull and stereotyped reactor of 94h
4Cells/ml, cylindrical reactor are 269 * 10
4Cells/ml, the former improves than the latter and 0.35 times.
More than two groups of experimental results illustrate once more because more piece-air lift type-inner light source-dull and stereotyped bioreactor light path is short, and adopted special more piece-air lift type structure, promoted of the mixing of algae liquid greatly at direction of illumination, help the growth of little algae.Owing to add in the experiment of artificial light group, cause high-cell density to be lower than the natural lighting group on the contrary a little less than the natural lighting relatively, but it is bigger than the multiple that cylindrical reactor improves to add the high-cell density of artificial light group experiment middle plateform reactor, and this illustrates that dull and stereotyped reactor source of artificial light modes of emplacement (internal light source) has improved the illumination utilising efficiency of reactor significantly.So more piece-air lift type-inner light source-special internal structure of dull and stereotyped bioreactor has promoted the mixing of algae liquid at direction of illumination, has improved the efficiency of light energy utilization, more helps the growth of frustule.
In sum, 1000L more piece-air lift type-inner light source-dull and stereotyped bioreactor is guidance with advanced person's bioreactor design concept, concentrated the advantage of flat bioreactor and column type bioreactor, and novelty be provided with in the reactor independently hollow cavity, place internal light source, avoided the shortcoming of simple built-in and external light source, hollow cavity is simultaneously as the dividing plate that improves the inside reactor mixed effect, both promoted of the mixing of algae liquid greatly at direction of illumination, strengthened the utilising efficiency of illumination, avoid the deficiency of traditional inner light source bioreactor again, improved the culture efficiency of little algae greatly.
Although the invention describes concrete example, having a bit is significantly to those skilled in the art, promptly can do various variations and change to the present invention under the premise without departing from the spirit and scope of the present invention.Therefore, claims have covered all these changes within the scope of the present invention.It is for referencial use that this paper is all included in all publications, patent and the patent application that this paper quotes in.
Claims (10)
1. a dull and stereotyped bioreactor is characterized in that, described dull and stereotyped bioreactor comprises:
Transparent flat casing;
At described box house a plurality of hollow flow apron cavitys are arranged, described flow apron inside cavity is equipped with one or more source of artificial light, and described flow apron cavity is installed on the interior vertical plane of described reactor, has between each flow apron cavity at interval; With
The device of supply gas.
2. dull and stereotyped bioreactor as claimed in claim 1 is characterized in that, is spaced apart 10-100mm between described each flow apron cavity.
3. dull and stereotyped bioreactor as claimed in claim 1 is characterized in that, the cumulative volume of described dull and stereotyped bioreactor is 1000L or bigger.
4. dull and stereotyped bioreactor as claimed in claim 1 is characterized in that, the device of described supply gas is the gas distributor at described reactor bottom.
5. dull and stereotyped bioreactor as claimed in claim 1, it is characterized in that, described flow apron cavity is installed on the interior same vertical plane of described reactor, this vertical plane is divided into fluid rising passway and fluid decline passway with the inside reactor zone, and the device of described supply gas is in described fluid rising passway.
6. dull and stereotyped bioreactor as claimed in claim 5 is characterized in that, the ratio of the sectional area of described fluid rising passway and described fluid decline passway is 0.5: 1 to 3: 1.
7. dull and stereotyped bioreactor as claimed in claim 1, it is characterized in that, described flow apron cavity is installed on the interior a plurality of vertical planes of described reactor, this vertical plane is divided into a plurality of fluid rising passwaies and fluid decline passway with the inside reactor zone, and the device of described supply gas is in described fluid rising passway one or more.
8. dull and stereotyped bioreactor as claimed in claim 1 is characterized in that, described dull and stereotyped bioreactor also comprises the device of controlled temperature, the device that detects pH, the device that detects dissolved oxygen, device and the charging and the discharge device of detected temperatures.
9. dull and stereotyped bioreactor as claimed in claim 1 is characterized in that, the source of artificial light in the described flow apron cavity links to each other with the light path control system.
10. the application of each described dull and stereotyped bioreactor of claim 1-9 in cultivating little algae.
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