WO2014176852A1

WO2014176852A1 - Microorganism culture device

Info

Publication number: WO2014176852A1
Application number: PCT/CN2013/083232
Authority: WO
Inventors: 钟琦
Original assignee: Zhong Qi
Priority date: 2013-04-28
Filing date: 2013-09-10
Publication date: 2014-11-06
Also published as: CN103224875A

Abstract

Provided is a microorganism culture device, comprising a culture box and a bubble generator; the culture box is provided with a bubble guiding structure therein for extending the bubble path of movement, and bubbles with nutrient content are guided by the bubble guiding structure to move upwards, thus increasing the contact area between the bubbles and a microorganism culture medium and extending the contact time, so as to allow the gaseous nutrients to be fully transported to the microorganism culture medium; and further driving movement of the microorganism culture medium, and discharging the metabolite of microorganism growth, so as to reduce energy consumption during microorganism culturing, improve culture efficiency, and reduce culture cost.

Description

t biological culture device

Technical field

The invention relates to the technical field of microbial culture, and in particular to a microbial culture device. Background technique

In existing microbial cultures, gaseous nutrients are typically delivered to the microbial culture fluid from the atmosphere or in an artificial gas storage container by means of bubbles. Increasing the supply of gaseous nutrients in the microbial culture fluid is beneficial to the growth of microorganisms. At present, there are three ways to increase the supply of gaseous nutrients: The first is to increase the gas transmission rate, and put more gas into the microbial incubator for a certain period of time; the second is to reduce the diameter of the bubble, so that the bubble can be dissolved In the culture solution; the third is to increase the height of the microbial culture solution experienced by the bubbles in the incubator. The disadvantages of these three methods are: 1 will increase the energy consumption of the microbial culture process, thereby increasing the production cost; 2 can not enlarge the mode of the microbial incubator to the scale of industrial production; 3 can not use the microbial culture with only a small amount of microbial culture solution The culture is limited, and the application of the microbial incubator is limited. 4 For microalgae cultivation, the prior art can only provide carbon dioxide gas and light energy to a small part of microalgae cells for photosynthesis, thereby reducing the culture efficiency.

Summary of the invention

The object of the present invention is to provide a microorganism culture apparatus which is safe, reliable, low in energy consumption, high in efficiency, and wide in application range in view of the above problems and deficiencies.

The microorganism culture apparatus according to the present invention comprises an culture chamber for holding a microbial culture liquid at the top, a lid for sealing the lid on the top opening of the culture tank, and being placed in the culture tank through the intake pipe and A bubble generator connected to a gas pump outside the incubator for supplying a microbial culture solution with a bubble containing a nutrient gas required for growth of the microorganism culture, and a bubble generator disposed on the lid and communicating with the inner chamber of the culture chamber The air bubble collecting the microbial metabolites is discharged from the air outlet pipe of the culture box, and is characterized in that the incubator body is provided with a bubble guiding structure for extending the bubble traveling path, and the bubble generator is located at the bottom of the bubble guiding structure.

In order to ensure sufficient and balanced nutrient supply in the whole microbial culture solution, the above-mentioned bubble guiding structure comprises at least one microbial culture liquid for bubbles and bubbles to be driven from bottom to top and longer than the bubble directly rising from the bottom of the microbial culture solution. a passage to the path of the surface of the microbial culture solution, and between the bubble guiding structure and the inner wall of the culture tank, a microbial culture solution for pushing the bubble to the top is returned from the top to the bottom of the incubator gap. The channel may be a channel for the microbial culture liquid driven by the bubble and the bubble to travel from the bottom to the zigzag, or may be a channel of other shapes. In order to enable the present invention to achieve the purpose of extending the bubble travel path, the bubble guiding structure is composed of at least one structure for changing the direction of bubble movement and impeding the bubble, the structure being made of plastic or metal or Made of glass or other material that is not water-soluble.

When the bubble guiding structure is composed of a plurality of structures, in order to make the structure and the mounting method of the present invention various, the structures may be respectively mounted on a support frame, or the structures may be Installed on the intake ducts respectively, one of the upper and lower adjacent two structures of the structures may be mounted on one support frame, the other one is installed on the intake duct, or may be the respective structures. The objects are respectively mounted on the inner wall of the culture box, and the structures may be connected to each other and then fixed to a support frame. Moreover, each of the structures is disposed horizontally or obliquely. Moreover, each of the structures is a planar baffle or a conical baffle or other geometric baffle.

In order to make the present invention effectively extend the bubble traveling path, the microorganism has an efficient photosynthesis and a rapid growth rate, and an artificial light source is mounted on the bubble guiding structure or the incubator.

The present invention adopts a bubble guiding structure in which an air bubble walking path can be extended in the incubator body, and when the microorganism culture is performed, by using the bubble guiding structure, bubbles rich in carbon dioxide or oxygen can be guided by the bubble at the bottom of the bubble guiding structure. The path set by the structure moves from bottom to top, which slows the vertical rising speed of the bubble and increases the horizontal movement, thereby effectively increasing the contact area of the bubble with the microorganism culture solution and prolonging the contact time, so that the microorganism grows. The required gaseous nutrients can be fully transferred from the gas phase to the microbial culture solution for microbial growth, which greatly reduces the energy consumption during the microbial culture process, improves the culture efficiency and reduces the culture cost, and simultaneously promotes the movement of the bubbles. The movement of the microbial culture solution, which transfers the nutrients required for the growth of the microorganisms to the entire microbial culture solution and excretes the metabolites of the microbial growth through the bubbles, and the highly efficient absorption of the gaseous nutrients is beneficial to Cultivation of various microorganisms . At the same time, an artificial light source can be installed on the bubble guiding structure. When cultivating microorganisms capable of photosynthesis, the artificial light source can project light energy around the bubble so that the microbial cells can simultaneously obtain the carbon dioxide and light energy necessary for photosynthesis. Thereby the microorganism can effectively carry out photosynthesis and rapid growth. In addition, a gap is left between the bubble guiding structure and the inner wall of the incubator, and the microbial culture liquid which is raised by the bubble to the top is returned from the top to the bottom of the incubator, and the microbial culture liquid is in the incubator. The body forms an up and down circular motion, thereby effectively ensuring the nutritional balance in the entire microbial culture solution. Moreover, due to the sealed connection between the incubator and the lid, the sealed microbial culture device can avoid the pollution of the microorganisms in the cultured air, and the microbial culture. The effect is better.

DRAWINGS

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a schematic cross-sectional view showing a first embodiment of the present invention.

Fig. 2 is a schematic exploded view showing the first embodiment of the present invention.

Figure 3 is a schematic cross-sectional view showing a second embodiment of the present invention.

Figure 4 is a schematic exploded view of Embodiment 2 of the present invention.

Figure 5 is a schematic cross-sectional view showing a third embodiment of the present invention.

Figure 6 is a schematic exploded view of Embodiment 3 of the present invention.

Figure 7 is a schematic cross-sectional view showing the fourth embodiment of the present invention.

detailed description

The invention will now be further described with reference to the drawings and specific embodiments.

As shown in FIG. 1 to FIG. 7 , the microorganism culture apparatus according to the present invention comprises an culture chamber 1 for holding a microbial culture solution 12 at the top, and a lid 2 for sealing the lid on the top opening of the culture cabinet 1. The bubble generation in the culture tank 1 and communicating with the air pump 4 located outside the culture tank 1 through the intake duct 3 for supplying the microbial culture solution 12 with the air bubbles 11 containing the nutrient gas required for the growth of the microorganism culture And an air outlet duct 6 disposed on the tank cover 2 and communicating with the inner cavity of the culture tank 1 for discharging the microbial metabolites, wherein the air inlet duct 3 is used to connect the air bubbles One end of the generator 5 may extend into the incubator 1 from the top opening of the incubator 1 or may protrude from the other part of the incubator 1 into the incubator 1 in order to effectively increase the bubbles. 11 contact area with the microbial culture solution 12 and prolonged contact time, the gaseous nutrients required for microbial growth can be effectively transferred from the gas phase to the microbial culture solution 12 for microbial growth, thereby reducing the microbial culture process. The energy consumption in the medium is increased, and the culture efficiency is lowered, and the culture cost is lowered. The bubble guiding structure 7 for extending the traveling path of the bubble 11 is provided in the culture tank 1, and the bubble generator 5 is located at the bottom of the bubble guiding structure 7. In order to ensure sufficient and balanced nutrient supply in the whole microbial culture solution, the bubble guiding structure 7 contains at least one microbial culture solution for the bubble 11 and the bubble 11 to move from bottom to top and longer than the bubble 11 directly from the microbial culture solution. The channel 9 which rises vertically to the path of the surface of the microbial culture solution, and the channel 9 can be a channel for the microbial culture liquid pushed by the bubble 11 and the bubble 11 to run from the bottom to the zigzag, or other shapes. The passage, and the bubble guiding structure 7 and the inner wall of the incubator 1 are left with a gap in which the microbial culture liquid which can be pushed up to the top by the bubble 11 is returned from the top to the bottom of the incubator 1. In order to enable the present invention to achieve the purpose of extending the traveling path of the bubble 11 and effectively improving the utilization rate of the microbial culture solution, the bubble guiding structure 7 is composed of at least one bubble for changing the direction of movement of the bubble 11. 11 is constructed of an inaccessible structure 71, and the structure 71 is made of plastic or metal or glass or other material that is not water-tolerant. When the bubble guiding structure 7 is composed of only one structure 71, the structure 71 may be a spiral structure made of a single plate, the bottom surface of which has an arcuate groove, or the structure 71 It is a folded bag-like structure. However, in practical applications, as shown in FIGS. 1 to 7, the bubble guiding structure 7 is generally composed of a plurality of structures 71, and in order to make the structure and the mounting method of the present invention various, it is possible to be a structure. 71 are respectively mounted on a support frame 8, or each structure 71 may be respectively mounted on the intake duct 3, and at this time, the intake duct 3 is perpendicular to the bottom surface of the incubator 1, and may also be the upper and lower sides of each structure 71. One of the adjacent two structures 71 is mounted on a support frame 8 and the other is mounted on the intake duct 3. Alternatively, each of the structures 71 may be mounted on the inner wall of the culture cabinet 1, or may be each The structures 71 are connected to each other and then fixed to a support frame 8. Moreover, each of the structures 71 is disposed horizontally or obliquely. Also, each of the structures 71 is a planar baffle or a conical baffle or a stepped baffle or other geometrical baffle. In general, when each of the structures 71 is a flat baffle, each of the structures 71 is disposed obliquely; and when each of the structures 71 is a conical baffle, each of the structures 71 is horizontally disposed. In addition, the culture box 1 and the cover 2 are respectively made of plastic or metal or glass or other materials, and the shape of the culture box 1 may be rectangular or circular or other geometric shape, the shape of the cover 2 and the culture box 1 The shape of the bubble generator 5 can be any shape. In order to make the present invention effectively extend the walking path of the bubble 11, the microorganism has high photosynthesis and rapid growth rate, and the artificial light source 10 is mounted on the bubble guiding structure 7 or the incubator 1 It can emit light sources with wavelengths from 400 to 700 nm. As shown, at least one artificial light source 10 is disposed on the bottom surface of each of the structures 71 constituting the bubble guiding structure 7.

As shown in FIG. 1 and FIG. 2, in this embodiment, the structure 71 is a planar baffle, and the planar baffle is provided with a plurality of blocks, and the planar baffles are arranged up and down and obliquely connected to each other to form a zigzag structure. The zigzag structure is fixed on a support frame 8 and suspended into the incubator 1 through the support frame 8, and the planar baffles of the two adjacent planar baffles are located at the junction of the two planar baffles An exhaust hole 711 is provided on the inner side of the bottom surface of the planar baffle that allows the bubble 11 to rise from the bottom surface of the lower planar baffle. As shown in the figure, a socket 713 is defined at a position between the end of each of the planar baffles adjacent to the exhaust hole 711 and the exhaust hole 711, and a pin 714 is disposed at an end away from the exhaust hole 711, and the support frame is provided. The support portion of the 8 is arranged to be inclined up and down. When the planar baffles are integrally connected to each other, the planar baffle located at the bottommost surface is first placed on the support portion of the support frame 8, and then the remaining planar baffles are The bottom and the top are plugged together one by one, and a channel 9 through which the air bubbles 11 pass is formed between the adjacent two planar baffles. Moreover, in each block of the baffle Grooves for engaging the artificial light source 10 are respectively disposed on the bottom surface, or the artificial light source 10 is connected and fixed on the bottom surface of the planar baffle by a connecting member such as a screw. At the same time, one end of the air inlet duct 3 to which the bubble generator 5 is connected is inserted from the top opening of the incubator 1 to place the bubble generator 5 to the inner bottom of the incubator 1.

As shown in FIG. 3 and FIG. 4, in this embodiment, the structure 71 is a planar baffle, and the planar baffle is provided with a plurality of blocks, and the planar baffles are respectively vertically and obliquely and obliquely mounted on a support frame 8 and The support frame 8 is fixed into the culture box body 1, and two adjacent flat baffles are formed between the upper end of the lower planar baffle and the lower end of the planar baffle located above, and the air bubbles 11 are formed by the lower support frame. 8 is composed of a bottom plate and two vertical columns arranged on the bottom plate, and each of the planar baffles is respectively provided with an inclined groove for inserting the flat baffles, or the flat baffles are connected by screws and the like. Tiltly attached to the two columns. When the planar baffles are placed in the incubator 1 along with the support frame 8, a gap may be left between the two columns and the inner wall of the incubator 1, and the lower end edge of each planar baffle and the culture box 1 There may also be a gap or an inner side of the lower end between the inner walls to form an exhaust space 712 between the bottom surfaces of the planar baffles for raising the air bubbles 11 from the bottom surface of the lower planar baffle. A passage 9 through which the air bubbles 11 pass is formed between the adjacent two planar baffles. Further, a groove for engaging the artificial light source 10 is formed on the bottom surface of each of the planar baffles, or the artificial light source 10 is fixed to the bottom surface of the planar baffle by a connecting member such as a screw. At the same time, one end of the air inlet pipe 3 to which the bubble generator 5 is connected is inserted from the top opening of the incubator 1 to place the bubble generator 5 to the inner bottom of the incubator 1.

As shown in FIG. 5 and FIG. 6, in this embodiment, the structure 71 is a conical baffle, and the conical baffle is provided with a plurality of blocks, and the conical baffles are respectively mounted coaxially and vertically spaced apart. It is fixed to the incubator 1 on the intake duct 3 and a support frame 8 and through the intake duct 3 and the support frame 8, and one of the adjacent two conical baffles is mounted on the support frame 8. The other block is mounted on the intake duct 3, and the center of the conical baffle mounted on the support frame 8 is provided with a vent hole 711 through which the air bubble 11 can pass from bottom to top. Wherein, the support frame 8 is composed of a bottom plate and two vertical columns disposed on the bottom plate, and the conical baffle mounted on the support frame 8 is mounted on the two vertical columns and located between the two vertical columns, and is conical The baffle is mounted on the two columns, either horizontally for the two conical baffles to be inserted into the two conical baffles, or by connecting the conical baffles with screws or the like. The pieces are horizontally connected and fixed to the two columns. The center of the conical baffle mounted on the intake duct 3 is provided with a connection matching the outer diameter of the intake duct 3. The hole is fixedly connected to the intake duct 3 through the connecting hole and is connected to the intake duct 3, and the outer end of the conical baffle installed in the intake duct 3 is located on the circle mounted on the support frame 8. The inner side of the outer end of the conical baffle is such that the outer end of the conical baffle mounted on the intake duct 3 has an exhaust space 712 through which the air bubbles 11 pass downwardly, and adjacent two circles A passage 9 through which the air bubbles 11 pass is formed between the tapered baffles, and a gap is left between the conical baffles and the inner wall of the incubator 1. Moreover, a groove for engaging the artificial light source 10 is opened on the bottom surface of each of the conical baffles, or the artificial light source 10 is connected and fixed to the bottom surface of the conical baffle by a connecting member such as a screw.

As shown in FIG. 7 , in this embodiment, the structure 71 is a planar baffle, and the planar baffle is provided with a plurality of blocks, and the planar baffles are respectively vertically and obliquely and obliquely mounted on the inner wall of the incubator 1 , and Two upper planar baffles are formed between the upper end of the lower planar baffle and the lower end of the upper planar baffle to form a bottom surface of the planar baffle for raising the air bubble 11 from the bottom surface of the lower planar baffle An exhaust space 712 is formed, and a passage 9 through which the air bubbles 11 pass is formed between adjacent two planar baffles. At the same time, the intake duct 3 is penetrated from the through hole opened in the side wall of the incubator 1 to communicate with the bubble generator 5 placed in the incubator 1, and is provided on the bottom surface of each plane baffle for The recess of the artificial light source 10 is embedded, or the artificial light source 10 is connected and fixed to the bottom surface of the planar baffle by a connecting member such as a screw. The connection is integrated and then placed into the incubator 1. For example, the structure 71 is a planar baffle, and the planar baffle is provided with a plurality of blocks, and the planar baffles are respectively vertically and obliquely and obliquely mounted on the intake duct 3 And is fixed in the incubator 1 through the intake duct 3, and two adjacent flat baffles are formed between the upper end of the lower planar baffle and the lower end of the planar baffle located above, and the air bubble 11 is formed by the object 71 The conical baffle is provided with a plurality of blocks, and the conical baffles are respectively coaxially and vertically spaced on the intake duct 3 and fixed to the incubator 1 through the intake duct 3 Inside, and one of the adjacent two conical baffles has a vent hole 711 through which the bubble 11 passes from the bottom to the center, and the outer end of the other block allows the bubble 11 to pass from the bottom to the top. Exhaust space 712 through. Further, each of the structures 71 and the incubator 1 may be directly connected together by other connection means.

When the present invention is used, the air pump 4 feeds a nutrient gas containing microorganisms, for example, a gas containing carbon dioxide or oxygen, from the intake duct 3 into the bubble generator 5, so that the gas is in the form of bubbles 11 from the bubble generator 5. Entering the microbial culture solution starts to move from bottom to top in the channel 9 formed by the structure 71, and when the structure 71 is hit, the bubble 11 can only change the direction of movement. The horizontal and upward movements are simultaneously performed along the guiding of the structure 71, and can be raised from the upper end edge or the outer end edge of one piece of the structure 71 to the bottom surface of the next block structure 71. In this exercise, when carbon dioxide is carried in the bubble 11, the carbon dioxide is absorbed by the microorganism culture solution, and the biological cells in the vicinity of the structure 71 use the artificial light source 10 and the carbon dioxide absorbed by the biological culture solution to perform photosynthesis to release oxygen. At the same time, the bubble 11 also collects the oxygen generated by the photosynthesis of the microbial cells, and the movement of the bubble 11 drives the bottom-up movement of the microbial culture solution in the channel 9, while being carried by the bubble 11 to the top. The microbial culture solution is returned from the top to the bottom of the incubator 1 from the gap between the bubble guiding structure 7 and the incubator 1 to form a circulating motion of the microbial culture solution, thereby ensuring the entire microbial culture solution. Nutritional balance. Finally, after the bubble 11 emerges from the water surface, the culture tank 1 is released from the gas outlet pipe 6; when the air bubble 11 carries oxygen, the oxygen is absorbed by the microorganism culture liquid, and the microbial cells in the vicinity of the structure utilize the microorganism culture liquid. The absorbed oxygen undergoes metabolic activity. At the same time, the bubble 11 also collects the carbon dioxide released by the metabolism of the microbial cells, and the movement of the bubble 11 in the channel 9 drives the bottom-up movement of the microbial culture solution, and the microbial culture carried by the bubble 11 to the top. The liquid will again flow from the top to the bottom of the incubator 1 from the gap between the bubble guiding structure 7 and the incubator 1 to form a circulating motion of the microbial culture solution, thereby ensuring the nutritional balance in the entire microbial culture solution. . Finally, after the bubble 11 emerges from the water surface, the culture chamber 1 is released from the air outlet duct 6.

The present invention is described by way of example, but not by way of limitation of the invention, the description of the invention, and other variations of the disclosed embodiments, as will be readily apparent to those skilled in the art, It is within the scope of the claims of the present invention.

Claims

Claim

A microorganism culture apparatus comprising an culture tank for containing a microorganism culture solution (12)

(1) A bubble generator (5) for supplying a bubble (11) containing a nutrient gas required for growth of microorganisms to a microorganism culture solution (12), characterized in that: the culture chamber

(1) A bubble guiding structure (7) for extending the traveling path of the bubble (11) is provided, and the bubble generator (5) is located at the bottom of the bubble guiding structure (7).

2. The microorganism culture apparatus according to claim 1, wherein: the bubble guiding structure (7) comprises at least one microbial culture liquid capable of pushing the air bubbles (11) and the air bubbles (11) from bottom to top. The passage (9) that travels longer than the bubble (11) directly from the bottom of the microbial culture to the path traveled by the surface of the microbial culture.

3. The microorganism culture apparatus according to claim 2, wherein: the bubble guiding structure (7) and the inner wall of the culture tank (1) are left with microorganisms that can be pushed to the top by the bubble (11) The culture solution is refluxed from top to bottom to the gap at the bottom of the culture chamber (1).

The microbial culture device according to claim 2, wherein: the bubble guiding structure (7) comprises a planar baffle, the planar baffle is provided with a plurality of blocks, and the planar baffles are arranged up and down and Tiltly connected to each other in a zig-zag structure, the zig-zag structure is fixed on a support frame (8) and suspended in the incubator (1) through the support frame (8), and two adjacent planar baffles The lower planar baffle is provided at the inner side of the joint of the two planar baffles with a venting opening (711) for allowing the bubble (11) to rise from the bottom surface of the lower planar baffle to the bottom surface of the planar baffle.

The microbial culture device according to claim 2, wherein: the bubble guiding structure (7) comprises a planar baffle, the planar baffle is provided with a plurality of blocks, and the planar baffles are vertically spaced apart and Mounted obliquely on a support frame (8) and passed through the support frame

(8) is fixed in the incubator (1), and an air bubble (11) is formed between the upper end of the lower planar baffle and the lower end of the planar baffle located above

(712).

6. The microorganism culture apparatus according to claim 2, wherein: the bubble guiding structure (7) comprises a planar baffle, and the planar baffle is provided with a plurality of blocks, the planes The baffles are respectively vertically and obliquely and obliquely mounted on the inner wall of the incubator body (1), and two adjacent planar baffles are formed between the upper end of the lower planar baffle and the lower end of the planar baffle located above The supply air bubble (11) is raised from the bottom surface of the lower planar baffle to the exhaust space (712) of the bottom surface of the upper planar baffle.

The microbial culture device according to claim 2, wherein: the bubble guiding structure (7) comprises a planar baffle, the planar baffle is provided with a plurality of blocks, and the planar baffles are vertically spaced apart and Tiltly mounted on the intake duct (3) and through the intake duct

(3) is fixed in the incubator (1), and an air bubble is formed between the upper end of the lower planar baffle and the lower end of the planar baffle located above (11)

(712).

8. The microorganism culture apparatus according to claim 2, wherein: the bubble guiding structure (7) comprises a conical baffle, and the conical baffle is provided with a plurality of pieces, each of the conical shapes The baffles are respectively mounted coaxially and vertically on the intake duct (3) and a support frame

(8) is fixed in the incubator (1) through the intake pipe (3) and the support frame (8), and one of the adjacent two conical baffles is mounted on the support frame (8) The upper part and the other part are mounted on the air inlet duct (3), and the center of the conical baffle mounted on the support frame (8) is provided with a vent hole through which the air bubble (11) passes from bottom to top. (711), the outer end of the conical baffle mounted on the intake duct (3) leaves an exhaust space (712) through which the bubble (11) passes from bottom to top.

9. The microorganism culture apparatus according to claim 2, wherein: the bubble guiding structure (7) comprises a conical baffle, and the conical baffle is provided with a plurality of pieces, each of the conical shapes The baffles are respectively mounted coaxially and vertically spaced on the intake duct (3) and fixed in the incubator (1) through the intake duct (3), and one of the adjacent two conical baffles The center is provided with a vent hole (711) through which the bubble (11) passes from bottom to top, and the outer end of the other block has an venting space (712) through which the bubble (11) passes from bottom to top.

The microorganism culture apparatus according to any one of claims 1 to 9, characterized in that the artificial light source (10) is installed in the culture tank (1).