CN103224875A - Microorganism culture apparatus - Google Patents
Microorganism culture apparatus Download PDFInfo
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- CN103224875A CN103224875A CN201310154091XA CN201310154091A CN103224875A CN 103224875 A CN103224875 A CN 103224875A CN 201310154091X A CN201310154091X A CN 201310154091XA CN 201310154091 A CN201310154091 A CN 201310154091A CN 103224875 A CN103224875 A CN 103224875A
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- baffle plate
- bubble
- plane baffle
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Abstract
The invention relates to a microorganism culture apparatus, which comprises a culture box, a box cover, a gas pump, a gas inlet pipeline, a bubble generator and a gas outlet pipeline, wherein a bubble guiding structure for extending a bubble walking path is arranged inside the culture box. With the present invention, nutritional component-containing bubbles can move from the bottom of the bubble guiding structure according to a path set by the bubble guiding structure in a bottom-to-top movement manner so as to increase a contact area of the bubbles and a microorganism culture liquid, and prolong a contact time, such that gaseous nutrients required by microorganism growth can be completely delivered to the microorganism culture liquid from the gas phase to be used by microorganism growth; and the bubble movement drives the microorganism culture liquid movement, such that nutrients required by microorganism growth are uniformly delivered to the whole microorganism culture liquid and metabolites of microorganism growth are excreted through the bubbles with the movement so as to substantially reduce energy consumption during a microorganism culture process, improve culture efficiency and reduce culture cost.
Description
Technical field
The present invention relates to the microorganism culturing technical field, particularly relate to a kind of microbial cultivation device.
Background technology
In existing microorganism culturing device, the gasiform nutrition normally is delivered in the microbial culture medium from atmosphere or in the artificial gas storage vessel with the mode of bubble.Increase gaseous state nutrition being supplied with in microbial culture medium and be beneficial to microbial growth.At present, the method that increases the gaseous state nutrition supplement has three kinds: the firstth, and the transmission rate of increase gas is pressed into more gas in the microorganism culturing device within a certain period of time; The secondth, reduce the diameter of bubble, make bubble can be dissolved in the nutrient solution; The 3rd is the height that increases the microbial culture medium of bubble experience in the incubator.The shortcoming of these three kinds of methods is: 1. all can increase the energy expenditure of microbial cultivation process, thereby increase production cost; 2. the pattern of microorganism culturing device can not be amplified to the scale of suitability for industrialized production; 3. can not use the microorganism culturing device of only adorning a small amount of microbial culture medium to cultivate, limit the application of microorganism culturing device; 4. cultivate for little algae, prior art can only offer the sub-fraction microalgae cell to carbon dioxide and luminous energy simultaneously and carry out photosynthesis, has reduced culture efficiency.
Summary of the invention
The objective of the invention is at above-mentioned existing problems and deficiency, provide a kind of safe and reliable, energy consumption is low, efficient is high, the microbial cultivation device of applied range.
Technical scheme of the present invention is achieved in that
Microbial cultivation device of the present invention, comprise the open-topped cultivation casing that is used to hold microbial culture medium, sealing cover is put at the case lid of cultivating in the casing open top, be placed on cultivate in the casing and by the intake ducting air pump external being used for of being connected with being positioned at incubator to microbial culture medium the bubble generator of the bubble that contains the required nutrition gas of microorganism culturing growth is provided and be arranged on the case lid and with the outlet pipe that is used for a bubble of collecting microbial metabolites is discharged the cultivation casing of the intracavity inter-connection of cultivating casing, be characterized in being provided with the bubbles structure that is used to prolong the bubble walking path in the described cultivation casing, described bubble generator is positioned at the bottom of bubbles structure.
In order to ensure nutrition supply abundance and the balance in the whole microbial culture medium, include that but microbial culture medium that at least one air feed bubble and bubble promote is walked from lower to upper in the above-mentioned bubbles structure and be longer than bubble directly from the microbial culture medium bottom vertical rise to the microbial culture medium surface the passage of distance of process, and leave the gap that can be back to the cultivation bottom half from top to bottom between described bubbles structure and the inwall of cultivating casing for the microbial culture medium that is pushed into the top by bubble.And described passage both can be the microbial culture medium that air feed bubble and bubble promote be the passage of walking in a zigzag from lower to upper, also can be the passage of other shape.
For can simply being reached, the present invention reaches the purpose that prolongs the bubble walking path effectively, above-mentioned bubbles structure is used to change the bubble motion direction and the impenetrable structure of bubble is formed by at least one, and described structure is made by plastics or metal or glass or other material that is not soluted in water.
When above-mentioned bubbles structure is made up of some structures, in order to make structure of the present invention and mounting means varied, both can be that described each structure is installed in respectively on the bracing frame, also can be that described each structure is installed on the intake ducting respectively, can also be described each structure neighbouring two block structure things wherein one be installed on the bracing frame, another piece is installed on the intake ducting, can also be that described each structure is installed in respectively on the inwall of cultivating casing, can also be to be fixed on the bracing frame after described each structure interconnects again.And described each structure flatly is provided with respectively or is provided with obliquely.And described each structure is the baffle plate of plane baffle plate or coniform baffle plate or other geometrical shape.
In order to make the present invention when effectively having prolonged the bubble walking path, make microorganism have photosynthesis and growth rate efficiently fast, on above-mentioned bubbles structure or the cultivation casing source of artificial light is installed.
The present invention is because employing is placed with the bubbles structure that can prolong the bubble walking path in cultivating casing, when carrying out microorganism culturing, move from lower to upper by the path that the bubble that uses this bubbles structure to make to be rich in carbonic acid gas or oxygen is set according to the bubbles structure by the bottom of bubbles structure, the vertical uplift speed that makes bubble slows down and has increased the motion of horizontal direction, thereby increased the contact area of bubble and microbial culture medium effectively and prolonged duration of contact, the required gaseous state nutrition of microorganism growth can be delivered to the microbial culture medium from gas phase fully to be used for microorganism growth, so just greatly reduced the energy expenditure in the microbial cultivation process, improve culture efficiency and reduced the cultivation cost, motion of air bubbles has driven the motion of microbial culture medium simultaneously, the required nutrition of this moving handle microorganism growth is sent in the whole microbial culture medium equably and the meta-bolites of microorganism growth is drained by bubble, and the gaseous state nutritive substance of high-level efficiency absorption helps the cultivation of multiple microorganism.Simultaneously, can on the bubbles structure, source of artificial light be installed, when cultivation can be carried out photosynthetic microorganism, this source of artificial light makes microorganism cells can obtain simultaneously to carry out essential carbonic acid gas of photosynthesis and luminous energy around can projecting bubble to luminous energy, thereby makes microorganism can carry out photosynthesis and growth apace effectively.In addition, leave the gap between the inwall of bubbles structure and cultivation casing, the microbial culture medium that can make bubbles entrain rise to the top by these gaps is back to the bottom of cultivating casing from top to bottom, make microbial culture medium in cultivating casing, form cyclic motion up and down, thereby guarantee the nutritive equilibrium in the whole microbial culture medium effectively.Again owing to cultivate between casing and the case lid to being tightly connected, this closed type microbial cultivation device can avoid the air that pollutes in the culturing process that the cultivation of microorganism is caused interference, makes the culture effect of microorganism better.
The present invention is further illustrated below in conjunction with accompanying drawing.
Description of drawings
Fig. 1 is the cross-sectional view of embodiment of the present invention 1.
Fig. 2 is the decomposition texture synoptic diagram of embodiment of the present invention 1.
Fig. 3 is the cross-sectional view of embodiment of the present invention 2.
Fig. 4 is the decomposition texture synoptic diagram of embodiment of the present invention 2.
Fig. 5 is the cross-sectional view of embodiment of the present invention 3.
Fig. 6 is the decomposition texture synoptic diagram of embodiment of the present invention 3.
Fig. 7 is the cross-sectional view of embodiment of the present invention 4.
Embodiment
As Fig. 1-shown in Figure 7, microbial cultivation device of the present invention, comprise the open-topped cultivation casing 1 that is used for splendid attire microbial culture medium 12, sealing cover is put at the case lid of cultivating in casing 1 open top 2, be placed on cultivate in the casing 1 and by intake ducting 3 be positioned at being used for of cultivating that the outer air pump 4 of casing 1 is connected to microbial culture medium 12 bubble generator 5 of the bubble 11 that contains the required nutrition gas of microorganism culturing growth is provided and be arranged on the case lid 2 and with the outlet pipe 6 that is used for a bubble of collecting microbial metabolites is discharged cultivation casing 1 of the intracavity inter-connection of cultivating casing 1, wherein intake ducting 3 end that is used to connect bubble generator 5 both can extend into from the top open part of cultivating casing 1 and cultivate in the casing 1, also can be to extend into from other position of cultivating casing 1 to cultivate in the casing 1, for the contact area that increases bubble 11 and microbial culture medium 12 effectively and extend contact time, the required gaseous state nutrition of microorganism growth can be delivered to the microbial culture medium 12 from gas phase effectively to be used for microorganism growth, thereby the energy expenditure in the reduction microbial cultivation process, improve culture efficiency and reduce the cultivation cost, in cultivating casing 1, be provided with the bubbles structure 7 that is used to prolong bubble 11 walking paths, and bubble generator 5 is positioned at the bottom of bubbles structure 7.In order to ensure nutrition supply abundance and the balance in the whole microbial culture medium, include that but microbial culture medium that at least one air feed bubble 11 and bubble 11 promote is walked from lower to upper in the bubbles structure 7 and be longer than bubble 11 directly from the microbial culture medium bottom vertical rise to the microbial culture medium surface the passage 9 of distance of process, this passage 9 both can be that the microbial culture medium that air feed bubble 11 and bubble 11 promote is the passage of walking in a zigzag from lower to upper, also can be the passage of other shape, and leave between the inwall of bubbles structure 7 and cultivation casing 1 and can be back to the gap of cultivating casing 1 bottom from top to bottom for the microbial culture medium that is pushed into the top by bubble 11.For can simply being reached, the present invention reaches the purpose that prolongs bubble 11 walking paths effectively, and the utilization ratio that can improve microbial culture medium effectively, bubbles structure 7 is used to change bubble 11 direction of motion and bubble 11 impenetrable structures 71 are formed by at least one, and structure 71 is to be made by plastics or metal or glass or other material that is not soluted in water.When bubbles structure 7 just was made up of a structure 71, this structure 71 can be the helicoidal structure body of being made by the monolithic plate, and the bottom surface of this helicoidal structure body has arc groove, perhaps this structure 71 bag shaped structure that is foldings.But in the application of reality, extremely shown in Figure 7 as Fig. 1, bubbles structure 7 normally is made up of some structures 71, and in order to make structure of the present invention and mounting means varied, both can be that each structure 71 is installed in respectively on the bracing frame 8, also can be that each structure 71 is installed in respectively on the intake ducting 3, this moment, intake ducting 3 was vertical with the bottom surface of cultivating casing 1, can also be each structure 71 neighbouring two block structure things 71 wherein one be installed on the bracing frame 8, another piece is installed on the intake ducting 3, can also be that each structure 71 is installed in respectively on the inwall of cultivating casing 1, can also be to be fixed on the bracing frame 8 after each structure 71 interconnects again.And each structure 71 flatly is provided with respectively or is provided with obliquely.And each structure 71 is the baffle plate of plane baffle plate or conical baffle or stepped baffle plate or other geometrical shape.In general, when each structure 71 was the plane baffle plate, each structure 71 was provided with respectively obliquely; When each structure 71 was conical baffle, each structure 71 flatly was provided with respectively.In addition, cultivating casing 1 and case lid 2 is made by plastics or metal or glass or other material respectively, the shape of cultivating casing 1 can be rectangle or circular or other geometrical shape, and the shape of case lid 2 is corresponding with the shape of cultivating casing 1, and 5 of bubble generators can be Any shape.In order to make the present invention when effectively having prolonged bubble 11 walking paths, make microorganism have photosynthesis and growth rate efficiently fast, on bubbles structure 7 or cultivation casing 1, source of artificial light 10 is installed, but this source of artificial light 10 is the light source of emission wavelength in 400～700 nanometers.As shown in the figure, on the bottom surface of each structure 71 of forming bubbles structure 7, be provided with at least one source of artificial light 10.
As shown in Figures 1 and 2, in this embodiment, structure 71 is the plane baffle plate, this plane baffle plate is provided with some, each plane baffle plate is arranged up and down and is connected into a zigzag structure mutually obliquely, this zigzag structure is fixed on the bracing frame 8 and is hanging to by this bracing frame 8 and cultivates in the casing 1, but and adjacent two plane baffle plates plane baffle plate of being positioned at the below the inboard, junction of described two plane baffle plates be provided with air feed bubble 11 by below the venting hole 711 of bottom surface of the bottom surface of the plane baffle plate plane baffle plate above rising to.As shown in the figure, each plane baffle plate offers a jack 713 respectively near an end and the position between the venting hole 711 of venting hole 711, simultaneously be provided with a pin 714 at a end away from venting hole 711, and the structure that the support portion of bracing frame 8 is arranged to have a down dip, each plane baffle plate interconnects when being integral, at first be that the plane baffle plate that will be positioned at bottom surface is placed on the support portion of bracing frame 8, and then remaining plane baffle plate is plugged on together from lower to upper block by block, but be formed with air feed bubble 11 passages of walking 9 between the adjacent two plane baffle plates.And, on the bottom surface of each piece plane baffle plate, offer the groove that is used to rabbet source of artificial light 10 respectively, perhaps source of artificial light 10 is to be fastened on the bottom surface of plane baffle plate by web members such as screws.Simultaneously, to be connected with an end of bubble generator 5 be to stretch into and bubble generator 5 is placed into the inner bottom part of cultivating casing 1 from the top open part of cultivating casing 1 to intake ducting 3.
As shown in Figures 3 and 4, in this embodiment, structure 71 is the plane baffle plate, this plane baffle plate is provided with some, each plane baffle plate respectively between the upper and lower every and be mounted obliquely within on the bracing frame 8 and be fixed to and cultivate in the casing 1 by this bracing frame 8, but and adjacent two plane baffle plates below the plane baffle plate the upper end with above the lower end of plane baffle plate between be formed with air feed steep 11 by below the exhaust space 712 of bottom surface of the bottom surface of the plane baffle plate plane baffle plate above rising to.As shown in the figure, bracing frame 8 is made up of base plate and two root posts that are arranged on the base plate, each plane baffle plate respectively between the upper and lower every and be mounted obliquely within on two root posts and between two root posts, what can offer inclination this moment on two root posts is used to peg graft the groove of each plane baffle plate, or each plane baffle plate is fastened on two root posts obliquely by web members such as screws.When each plane baffle plate is put into cultivation casing 1 with bracing frame 8 after, can leave the gap between the inwall of two root posts and cultivation casing 1, and the lower edge of each plane baffle plate and cultivate and also can leave the gap between the inwall of casing 1 or both are in contact with one another, and but to be positioned at the lower end of plane baffle plate of top inboard and make and form air feed bubble 11 is risen to the plane baffle plate of top by the bottom surface of the plane baffle plate of below the exhaust space 712 of bottom surface between the two in the adjacent two plane baffle plates upper end of plane baffle plate that is in the below, but and be formed with air feed bubble 11 passages of walking 9 between the adjacent two plane baffle plates.And, on the bottom surface of each plane baffle plate, offer the groove that is used to rabbet source of artificial light 10, perhaps source of artificial light 10 is to be fastened on the bottom surface of plane baffle plate by web members such as screws.Simultaneously, to be connected with an end of bubble generator 5 be to stretch into and bubble generator 5 is placed into the inner bottom part of cultivating casing 1 from the top open part of cultivating casing 1 to intake ducting 3.
As Fig. 5 and shown in Figure 6, in this embodiment, structure 71 is a conical baffle, this conical baffle is provided with some, each conical baffle is coaxial respectively and be installed at interval in the upper and lower on an intake ducting 3 and the bracing frame 8 and be fixed to jointly by intake ducting 3 and bracing frame 8 and cultivate in the casing 1, and adjacent two conical baffles wherein one be mounted on the bracing frame 8, another piece is mounted on the intake ducting 3, but and the center that is installed in the conical baffle on the bracing frame 8 offer air feed bubble 11 venting holes that pass from lower to upper 711.Wherein, bracing frame 8 is made up of base plate and two root posts that are arranged on the base plate, being installed in conical baffle on the bracing frame 8 is mounted on two root posts and between two root posts, and conical baffle is installed on two root posts, both can be the level that on two root posts, offers be used to peg graft the groove of each conical baffle, also can be that each piece conical baffle flatly is fastened on two root posts by web members such as screws.And the center that is installed in the conical baffle on the intake ducting 3 offers connecting hole that the outside diameter with intake ducting 3 is complementary and receive on the intake ducting 3 by the connecting hole cover and be fixed together with intake ducting 3, and the outer end that is installed in the conical baffle of intake ducting 3 is positioned at the inboard of the outer end that is installed in the conical baffle on the bracing frame 8, but thereby make the outer end that is installed in the conical baffle on the intake ducting 3 leave air feed bubble 11 exhaust spaces that pass from lower to upper 712, but steep 11 passages of walking 9 and be formed with air feed between adjacent two conical baffles, leave the gap between the inwall of each conical baffle and cultivation casing 1 simultaneously.And, on the bottom surface of each conical baffle, offer the groove that is used to rabbet source of artificial light 10, perhaps source of artificial light 10 is to be fastened on the bottom surface of conical baffle by web members such as screws.
As shown in Figure 7, in this embodiment, structure 71 is the plane baffle plate, this plane baffle plate is provided with some, each plane baffle plate respectively between the upper and lower every and be mounted obliquely within on the inwall of cultivating casing 1, but and adjacent two plane baffle plates below the plane baffle plate the upper end and above the lower end of plane baffle plate between be formed with air feed bubble 11 by below the exhaust space 712 of bottom surface of the bottom surface of the plane baffle plate plane baffle plate above rising to, but and be formed with air feed bubble 11 passages of walking 9 between the adjacent two plane baffle plates.Simultaneously, intake ducting 3 be from cultivating that through hole that casing 1 sidewall offers penetrates and be placed on the bubble generator of cultivating in the casing 15 and be connected, and on the bottom surface of each plane baffle plate, offer the groove that is used to rabbet source of artificial light 10, perhaps source of artificial light 10 is to be fastened on the bottom surface of plane baffle plate by web members such as screws.
In addition, each structure 71 can also be to put into after the object of other shape also can connect as one by other mode of connection to cultivate in the casing 1 again, for example: structure 71 is the plane baffle plate, this plane baffle plate is provided with some, each plane baffle plate respectively between the upper and lower every and be mounted obliquely within on the intake ducting 3 and be fixed to and cultivate in the casing 1 by intake ducting 3, but and adjacent two plane baffle plates below the plane baffle plate the upper end with above the lower end of plane baffle plate between be formed with air feed steep 11 by below the exhaust space 712 of bottom surface of the bottom surface of the plane baffle plate plane baffle plate above rising to; Perhaps structure 71 is a conical baffle, this conical baffle is provided with some, each conical baffle is coaxial respectively and be installed at interval in the upper and lower on the intake ducting 3 and be fixed to by intake ducting 3 and cultivate in the casing 1, but but and the outer end that offers air feed bubble 11 venting holes that pass from lower to upper 711, another piece, wherein one the center of adjacent two conical baffles leave air feed bubble 11 exhaust spaces that pass from lower to upper 712.In addition, also can directly link together between each structure 71 and the cultivation casing 1 by other mode of connection.
When the present invention uses, air pump 4 is containing the required nutrition gas of microorganism growth, the gas that for example contains carbonic acid gas or oxygen, send into the bubble generator 5 from intake ducting 3, make gas enter beginning motion from lower to upper in the passage 9 that structure 71 forms the microbial culture medium from bubble generator 5 with the form of bubble 11, after running into structure 71, bubble 11 can only change direction of motion, do level simultaneously and move up along the guiding of structure 71, and can rise on the bottom surface of next block structure thing 71 from the upper end-face edge or the outer end edges of a block structure thing 71.In this motion, when carry in the bubble 11 be carbonic acid gas the time, carbonic acid gas can be absorbed by microbial culture medium, and near the microorganism cells the structure 71 utilizes source of artificial light 10 and carried out photosynthesis by the carbonic acid gas that microbial culture medium absorbs and emit oxygen simultaneously.At the same time, bubble 11 has also been collected the oxygen that photosynthesis produced of microorganism cells, and the motion of bubble 11 has driven from bottom to top the motion of microbial culture medium in passage 9, while can be back to the bottom of cultivating casing 1 from bubbles structure 7 and the gap location of cultivating between the casing 1 again from top to bottom by the microbial culture medium that bubble 11 brings to the top, thereby formed the cyclic motion of microbial culture medium, guaranteed the nutritive equilibrium in the whole microbial culture medium.At last, bubble 11 is emerged behind the water surface, is released out cultivation casing 1 from outlet pipe 6; When carry in the bubble 11 be oxygen the time, oxygen can be absorbed by microbial culture medium, near the microorganism cells utilization the structure is carried out Metabolic activity by the oxygen that microbial culture medium absorbs.At the same time, bubble 11 has also been collected the carbonic acid gas that the microorganism cells metabolism is discharged, and the motion of bubble 11 in passage 9 driven the motion from bottom to top of microbial culture medium, while can be back to the bottom of cultivating casing 1 from bubbles structure 7 and the gap location of cultivating between the casing 1 again from top to bottom by the microbial culture medium that bubble 11 brings to the top, thereby formed the cyclic motion of microbial culture medium, guaranteed the nutritive equilibrium in the whole microbial culture medium.At last, bubble 11 is emerged behind the water surface, is released out cultivation casing 1 from outlet pipe 6.
The present invention describes by embodiment, but the present invention is not construed as limiting, with reference to description of the invention, other variations of the disclosed embodiments, expect easily that as professional person such variation should belong within the claim restricted portion of the present invention for this area.
Claims (10)
1. microbial cultivation device, comprise the open-topped cultivation casing (1) that is used to hold microbial culture medium (12), sealing cover is put at the case lid of cultivating in casing (1) open top (2), be placed on cultivate in the casing (1) and by intake ducting (3) be positioned at being used for of cultivating that the outer air pump (4) of casing (1) is connected to microbial culture medium (12) bubble generator (5) of the bubble (11) that contains the required nutrition gas of microorganism culturing growth is provided and be arranged on that case lid (2) is gone up and with the outlet pipe (6) of a bubble of collecting microbial metabolites discharge cultivation casing (1) that is used for of the intracavity inter-connection of cultivating casing (1), it is characterized in that being provided with the bubbles structure (7) that is used to prolong bubble (11) walking path in the described cultivation casing (1), described bubble generator (5) is positioned at the bottom of bubbles structure (7).
2. according to the described microbial cultivation device of claim 1, it is characterized in that above-mentioned bubbles structure (7) but in include that microbial culture medium that at least one air feed bubble (11) and bubble (11) promote is walked from lower to upper and be longer than bubble (11) directly from the microbial culture medium bottom vertical rise to the microbial culture medium surface the passage (9) of distance of process, and leave between described bubbles structure (7) and the inwall of cultivating casing (1) and can be back to the gap of cultivating bottom the casing (1) from top to bottom for the microbial culture medium that is pushed into the top by bubble (11).
3. according to the described microbial cultivation device of claim 1, it is characterized in that above-mentioned bubbles structure (7) is used to change bubble (11) direction of motion and the impenetrable structure of bubble (11) (71) is formed by at least one, described structure (71) is made by plastics or metal or glass or other material that is not soluted in water.
4. according to the described microbial cultivation device of claim 3, it is characterized in that above-mentioned structure (71) is the plane baffle plate, described plane baffle plate is provided with some, described each plane baffle plate is arranged up and down and is connected into a zigzag structure mutually obliquely, this zigzag structure is fixed on that a bracing frame (8) is gone up and is hanging to by this bracing frame (8) and cultivates in the casing (1), but and adjacent two plane baffle plates plane baffle plate of being positioned at the below be provided with the venting hole (711) of the bottom surface of the plane baffle plate of air feed bubble (11) above rising to by the bottom surface of the plane baffle plate of below in the inboard, junction of described two plane baffle plates.
5. according to the described microbial cultivation device of claim 3, it is characterized in that above-mentioned structure (71) is the plane baffle plate, described plane baffle plate is provided with some, described each plane baffle plate respectively between the upper and lower every and be mounted obliquely within that a bracing frame (8) is gone up and be fixed to and cultivate in the casing (1) by this bracing frame (8), but and adjacent two plane baffle plates below the plane baffle plate the upper end with above the lower end of plane baffle plate between be formed with the exhaust space (712) that air feed steeps the bottom surface of (11) plane baffle plate above being risen to by the bottom surface of the plane baffle plate of below.
6. according to the described microbial cultivation device of claim 3, it is characterized in that above-mentioned structure (71) is the plane baffle plate, described plane baffle plate is provided with some, described each plane baffle plate respectively between the upper and lower every and be mounted obliquely within on the inwall of cultivating casing (1), but and adjacent two plane baffle plates below the plane baffle plate the upper end with above the lower end of plane baffle plate between be formed with the exhaust space (712) of the bottom surface of the plane baffle plate of air feed bubble (11) above rising to by the bottom surface of the plane baffle plate of below.
7. according to the described microbial cultivation device of claim 3, it is characterized in that above-mentioned structure (71) is the plane baffle plate, described plane baffle plate is provided with some, described each plane baffle plate respectively between the upper and lower every and be mounted obliquely within that intake ducting (3) is gone up and be fixed to and cultivate in the casing (1) by intake ducting (3), but and adjacent two plane baffle plates below the plane baffle plate the upper end with above the lower end of plane baffle plate between be formed with the exhaust space (712) that air feed steeps the bottom surface of (11) plane baffle plate above being risen to by the bottom surface of the plane baffle plate of below.
8. according to the described microbial cultivation device of claim 3, it is characterized in that above-mentioned structure (71) is a conical baffle, described conical baffle is provided with some, described each conical baffle is coaxial respectively and be installed at interval in the upper and lower that intake ducting (3) and a bracing frame (8) are gone up and be fixed to jointly in the cultivation casing (1) by intake ducting (3) and bracing frame (8), and adjacent two conical baffles wherein one be mounted on the bracing frame (8), another piece is mounted on the intake ducting (3), and be installed in bracing frame (8) but on the center of conical baffle offer the venting hole (711) that air feed bubble (11) passes from lower to upper, be installed in intake ducting (3) but on the outer end of conical baffle leave the exhaust space (712) that air feed bubble (11) passes from lower to upper.
9. according to the described microbial cultivation device of claim 3, it is characterized in that above-mentioned structure (71) is a conical baffle, described conical baffle is provided with some, described each conical baffle is coaxial respectively and be installed at interval in the upper and lower that intake ducting (3) is gone up and be fixed to by intake ducting (3) and cultivate in the casing (1), but and wherein one the center of adjacent two conical baffles offer venting hole (711) that air feed bubble (11) passes from lower to upper but, the outer end of another piece leaves air feed and steeps the exhaust space (712) that (11) are passed from lower to upper.
10. according to the described microbial cultivation device of claim 1, it is characterized in that above-mentioned bubbles structure (7) or cultivate on the casing (1) source of artificial light (10) is installed.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310154091XA CN103224875A (en) | 2013-04-28 | 2013-04-28 | Microorganism culture apparatus |
| PCT/CN2013/083232 WO2014176852A1 (en) | 2013-04-28 | 2013-09-10 | Microorganism culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310154091XA CN103224875A (en) | 2013-04-28 | 2013-04-28 | Microorganism culture apparatus |
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| CN103224875A true CN103224875A (en) | 2013-07-31 |
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| CN201310154091XA Pending CN103224875A (en) | 2013-04-28 | 2013-04-28 | Microorganism culture apparatus |
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| CN (1) | CN103224875A (en) |
| WO (1) | WO2014176852A1 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103497894A (en) * | 2013-09-30 | 2014-01-08 | 钟琦 | Method for cultivating microorganisms |
| CN103525687A (en) * | 2013-09-30 | 2014-01-22 | 钟琦 | High-efficiency gas-liquid contact air bubble guiding assembly and microbial cultivation device |
| CN103525688A (en) * | 2013-09-30 | 2014-01-22 | 钟琦 | Bubble guide component, high-density microorganism culture device and applications |
| WO2014176852A1 (en) * | 2013-04-28 | 2014-11-06 | Zhong Qi | Microorganism culture device |
| WO2015042950A1 (en) * | 2013-09-30 | 2015-04-02 | 钟琦 | Bubble-guiding assembly, device for culturing high-density microorganisms and use thereof |
| CN112226347A (en) * | 2020-10-20 | 2021-01-15 | 中南大学 | Microorganism continuous culture device |
| CN113337386A (en) * | 2021-06-02 | 2021-09-03 | 枣庄科技职业学院 | Microbial fermentation equipment capable of simulating different illumination environments |
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| FI128860B (en) | 2019-07-22 | 2021-01-29 | Solar Foods Oy | Bioreactors for growing micro-organisms |
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| CN103525688A (en) * | 2013-09-30 | 2014-01-22 | 钟琦 | Bubble guide component, high-density microorganism culture device and applications |
| WO2015042950A1 (en) * | 2013-09-30 | 2015-04-02 | 钟琦 | Bubble-guiding assembly, device for culturing high-density microorganisms and use thereof |
| CN112226347A (en) * | 2020-10-20 | 2021-01-15 | 中南大学 | Microorganism continuous culture device |
| CN113337386A (en) * | 2021-06-02 | 2021-09-03 | 枣庄科技职业学院 | Microbial fermentation equipment capable of simulating different illumination environments |
| CN113337386B (en) * | 2021-06-02 | 2022-04-29 | 枣庄科技职业学院 | Microbial fermentation equipment capable of simulating different illumination environments |
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|---|---|
| WO2014176852A1 (en) | 2014-11-06 |
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