CN103525688A - Bubble guide component, high-density microorganism culture device and applications - Google Patents
Bubble guide component, high-density microorganism culture device and applications Download PDFInfo
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Abstract
The invention discloses a bubble guide component. The bubble guide component comprises at least two zigzag guide members arranged along guide plate. Each zigzag guide member comprises at least two guide plates which are up-down connected in the vertical direction. The guide plats are obliquely arranged relative to the horizontal plane. The oblique directions of any adjacent guide plates in the same zigzag guide member relative to the horizontal plane are opposite. A zigzag and rising channel is formed among any adjacent zigzag guide members. The projections of any guide plate of the zigzag guide member and the closest guide plate of the adjacent zigzag guide member in the horizontal plane are overlapped partially. The bubble guide component increases the area of bubble guide per unit volume greatly, realizes modular assembly, and has strong adaptability. The invention also discloses a high-density microorganism culture device which can realize large-scale production and applications in waste gas treatment and microalgae culture.
Description
Technical field
The present invention relates to microorganism culturing technical field, especially relate to a kind of bubbles assembly, high-density microbial cultivation device and the application of this device in microorganism culturing and off gas treatment.
Background technology
Modern air-polluting major cause is the excessive emissions of burning the industrial gaseous waste of industrial operation generation and the tail gas that the transportation means of multiple oil firing is emitted of gas by burning coal oil firing, its main component comprises harmful carbonic acid gas, nitrogen oxide, sulfur oxide and carbon monoxide and harmless nitrogen and water vapour.In atmospheric layer, having excessive carbonic acid gas can cause Greenhouse effect, and can make seawater souring, is the major cause that causes world environments to worsen.Though nitrogen oxide and sulfur oxide can not cause Greenhouse effect, these gases can become acid after meeting water, and acid rain is exactly to become acid formation after these gases are met water.Acid rain drops to earth's surface, can cause soil with organic matter to run off, impoverishing the soil, and can cause that earth surface water source acidifying makes hydrobiont reduce even disappearance, destroys ecotope.Acid rain is suspended in a kind of reason that still forms haze in the air, can cause serious atmospheric pollution.At present also do not have ripe exhaust gas treatment technology can control the discharge of carbonic acid gas.Developing direction for its treatment technology also the understanding that neither one is unified.It is exactly two kinds of representational study hotspots that seabed is buried with the solid carbon of algae.It is high that technical requirements is buried in seabed, and amount of investment is large.By contrast, the solid carbon of algae has basis widely, and many countries are all in the trial of carrying out the solid carbon of algae, and method is different, and result is different, but does not also break through so far.
Microorganism ring insurance system is with the reaction of microbial growth substituted chemistry, and allows microorganism that provided artificial environment is be provided to grow.Sulfur oxide in waste gas, nitrogen oxide and carbonic acid gas can naturally water-solublely be all that microorganism growth is used, and microorganism ring insurance system is not only for the absorption of gas but also carry out microorganism culturing.The center of microorganism ring insurance system is biological solid carbon, and the microorganism majority of selecting is various micro-algaes, and foster method for planting can be divided into open and closed.Openly support that to plant be mainly with racing track and pond, its advantage is to build and cost of use is low, be convenient to industrialization, easy cleaning; Its shortcoming is that production efficiency is low, easily contaminated, takes up an area greatly, and water consumption is many.Closed system is mainly various photoreactors, its advantage be production efficiency high, be difficult for contaminated, take up an area little, water consumption is few; Its shortcoming is to build and cost of use is high, easy cleaning, heavy industrialization application are comparatively difficult.As one of emphasis of research and development, industry member is being looked for always a kind ofly can have again high efficiency culture apparatus for suitability for industrialized production, but never new breakthrough for many years.The culture systems of existing micro-algae all can not meet the needs of solid carbon, does not also reach the object of micro-algae suitability for industrialized production.
In microbial cultivation process, conventionally Gaseous nutrition thing need to be delivered in the nutrient solution of microorganism the mode with bubble, especially photosynthetic microorganism is as algae, need to be in the situation that there is solar radiation and be rich in the gas of carbon, grow and carry out photosynthesis such as carbonic acid gas etc. in proper nutrition developing medium, thereby increase Gaseous nutrition thing being supplied with in microbial culture medium and be beneficial to microbial growth.At present, the method that increases the supply of Gaseous nutrition thing has three kinds: the firstth, and the transmission rate of increase gas, is pressed into more gas in microorganism culture environment within a certain period of time; The secondth, reduce the diameter of bubble, make bubble can be dissolved in nutrient solution; The 3rd is the height that increases the microbial culture medium of bubble experience in incubator.The shortcoming of these three kinds of methods is: 1. all can increase the energy expenditure of microbial cultivation process, thereby increase production cost; 2. the pattern of microbial culture garden can not be amplified to the scale of suitability for industrialized production; 3. can not use the microbial culture garden that only fills a small amount of microbial culture medium to cultivate, limit the application of microbial culture garden; 4. for micro-algae, cultivate, prior art can only offer sub-fraction microalgae cell carbon dioxide and luminous energy simultaneously and carry out photosynthesis, has reduced culture efficiency.
Summary of the invention
The object of the invention is to the problem and shortage existing for prior art, provide that a kind of energy consumption is low, efficiency is high, bubbles assembly and the micro-raw culture apparatus of high-density and application that can modularization assembling.
Technical scheme of the present invention is achieved in that a kind of bubbles assembly, comprises the tortuous ways that at least two along continuous straight runs are arranged; Described tortuous ways comprises that at least two at vertical direction successively connected guide plate up and down, described guide plate relative level face tilt arranges, and the vergence direction of the guide plate relative level face of arbitrary neighborhood is contrary in same tortuous ways, between the tortuous ways of arbitrary neighborhood, form the tortuous passage rising; Arbitrary guide plate of described tortuous ways and the hithermost guide plate of adjacent tortuous ways are overlapping in the projection section of horizontal plane.
Described bubbles assembly also comprises the mounting block for fixing described tortuous ways.
Bubbles assembly of the present invention has greatly increased the area of bubbles in unit volume, and has realized modularization, can assemble easily as required, and the mass-producing that can be applied to microorganism is cultivated.
As further improvement, described guide plate is smooth plate or arc or waviness plate.When described guide plate is smooth plate, the angle of itself and horizontal plane is for being preferably 10～30 °.
As further improvement, adjacent described tortuous ways be spaced apart 15-50mm.
The present invention also provides a kind of high-density microbial cultivation device, described high-density microbial cultivation device comprises container, bubble generator, the bubbles assembly described at least one, described bubble generator and bubble guidance set are arranged in described container, and described bubble generator is arranged on the bottom of bubbles assembly.
Further, between described bubbles assembly and the inwall of described container, leave for the mobile gap of nutrient solution.On described tortuous ways or on the inwall of container, also light source can be set, for example LED lamp.
The present invention also provides the application of described high-density microbial cultivation device in off gas treatment, the method of described off gas treatment comprises: in the container of high-density microbial cultivation device, add nutrient solution and introduce to comprise and using the microorganism of carbonic acid gas as carbon source, microorganism that can metabolism sulfur oxide, a kind of microorganism that causes less in can the microorganism of metabolism nitrogen oxide is cultivated, and described waste gas is imported to described bubble generator in described nutrient solution, form bubble, bubble enters described passage and climbs along the lower surface of guide plate from the bottom of described bubbles assembly, for microorganism provides gas nutrition.
The present invention also provides the application of described high-density microbial cultivation device in micro-algae is cultivated, be included in the container of high-density microbial cultivation device and add nutrient solution and introduce micro-algae and cultivate, and carbonated gas is imported to described bubble generator in described nutrient solution, form bubble, bubble enters described passage and climbs along the lower surface of guide plate from the bottom of described bubbles assembly, for microorganism provides gas nutrition, the light source arranging in culturing process provides luminous energy for micro-algae.
The invention has the beneficial effects as follows:
The bubbles assembly of special construction provided by the invention, greatly improves the area of bubbles, has realized modularization assembling.High-density microbial cultivation device of the present invention, the travel path that guide plate in bubbles assembly provides complications to make progress for the required bubble that carries nutrition gas of microbial cultivation process, make bubble along in the up process of guide plate lower surface, vertical velocity slows down and has increased the motion of horizontal direction, increased exponentially the contact area of the interior bubble of unit volume and microbial culture medium and extended gas-liquid contact time, the required nutritional gas body of microorganism growth can be delivered to microbial culture medium for microorganism growth from gas phase fully, greatly improve material and the energy utilization efficiency in microbial cultivation process, thereby improved culture efficiency and reduced cultivation cost, the complications process of moving upward of bubble has increased gas-liquid agitation frequency simultaneously, driven the motion of microbial culture medium, the required nutrition of microorganism growth is sent in whole microbial culture medium equably, be beneficial to the culture efficiency that improves microorganism, and design and the assembling mode of bubbles assembly are flexible, can realize modularization, strong adaptability.This high-density microbial cultivation device is applied to off gas treatment and the cultivation of micro-algae, and throughput can reach the level of suitability for industrialized production.
Accompanying drawing explanation
Fig. 1 is the structural representation of the guide plate of tortuous ways bubbles assembly while being a plurality of;
Fig. 2 is the decomposing schematic representation of guide plate assembly parts of bubbles assembly while being square plate;
Fig. 3 is the structural representation of guide plate assembly parts of bubbles assembly while being square plate;
Fig. 4 is that the guide plate of tortuous ways is the decomposing schematic representation of two assemblies of bubbles when dull and stereotyped;
Fig. 5 is that the guide plate of tortuous ways is two bubbles unit construction schematic diagram when dull and stereotyped;
Fig. 6 is the structural representation of guide plate assembly parts of bubbles assembly while being arc;
Fig. 7 is the bubbles unit construction schematic diagram of the guide plate of tortuous ways while being two arcs;
Fig. 8 is the bubbles unit construction schematic diagram that adopts another kind of assembling mode;
Fig. 9 is the partial enlarged drawing A of Fig. 8;
Figure 10 is the structural representation of tortuous ways in the bubbles assembly of Fig. 8;
Figure 11 is the high-density microbial cultivation device cross-sectional view of the embodiment of the present invention;
Figure 12 is the high-density microbial cultivation device STRUCTURE DECOMPOSITION schematic diagram of the embodiment of the present invention;
Figure 13 is the stereographic map of the high-density microbial cultivation device of the embodiment of the present invention.
Embodiment
Below in conjunction with the drawings and the specific embodiments, the present invention is further described.
Fig. 1～Fig. 5 provides a kind of preferred implementation that facilitates the batch making of guide plate and the assembling of bubbles assembly.As shown in Figure 1, structural representation for bubbles assembly, bubbles assembly 1 comprises the tortuous ways 10 that a plurality of along continuous straight runs are equidistantly arranged, tortuous ways 10 comprises that polylith contacts connected guide plate 101 successively up and down at vertical direction, guide plate 101 relative level face tilts arrange, in same tortuous ways 10, the vergence direction of the guide plate 101 relative level faces of arbitrary neighborhood is contrary, between the tortuous ways 10 of arbitrary neighborhood, form the tortuous passage rising, and arbitrary guide plate 101 of tortuous ways 10 is overlapping in the projection section of horizontal plane with the hithermost guide plate 101 of adjacent tortuous ways 10, bubble climbs from the bottom of bubbles assembly admission passage and along the lower surface of guide plate 101.
In Fig. 1, the assembly method of bubbles assembly 1 is to obtain assembly parts after the guide plate of parallel spaced set in a plurality of horizontal directions 101 is fixed, then in vertical direction stack, obtain bubbles assembly 1, specifically, by 180 ° of assembly parts upsets, obtain another assembly parts, by the assembly parts stack before and after upset, guide plate 101 is contacted and relative level face tilt opposite direction, obtain bubbles assembly 1, after stack, can further fix, prevent that guide plate from moving.
As shown in Figure 2, while being square plate for guide plate, the decomposing schematic representation of the assembly parts of bubbles assembly.Assembly parts comprise guide plate 101 and mounting block, mounting block comprises horizontal stripe 111 and the wedge shape cushion cap 112 having from the horizontal by the oblique slot 1110 of acute angle, can further comprise fastening piece 113, on wedge shape cushion cap 112, there is the groove 1120 corresponding with horizontal stripe 111 sizes, the thickness of wedge shape cushion cap 112 is vertically highly identical with guide plate 101, and its face close with guide plate 101 is parallel with guide plate 101.
Fig. 3 is that guide plate is while being square plate, the structural representation of the assembly parts of bubbles assembly, horizontal stripe 111 fixed guide plates 101 are used respectively at the place ahead and rear at the guide plate 101 of a plurality of horizontal direction equal intervals setting, consecutive position at the guide plate 101 at most end two ends arranges wedge shape cushion cap 112, make 101 of the inclined-plane of wedge shape cushion cap 112 and the guide plates of end form passage, horizontal stripe 111 two ends are inserted respectively in 1120 on wedge shape cushion cap 112, through fastening piece 113, as screw, fix, obtain assembly parts.
Fig. 4 is that the guide plate of tortuous ways is two when dull and stereotyped, the decomposing schematic representation of bubbles assembly, and Fig. 5 is the structural representation of corresponding bubbles assembly.This bubbles assembly is obtained by the stack of two assembly parts, and the decomposing schematic representation of assembly parts and structural representation be referring to Fig. 2, Fig. 3, and 180 ° of assembly parts upsets are superimposed upon and on another assembly parts, obtain the bubbles assembly 1 in Fig. 5.Bubbles assembly in Fig. 1 can obtain in the bubbles assembly stack assembling in a plurality of Fig. 5.
In bubbles assembly 1 as shown in Figure 5, tortuous ways 10 is comprised of two guide plates 101, in adjacent tortuous ways 10, between the tortuous ways 10 of arbitrary neighborhood, form the tortuous passage 2 rising, arbitrary guide plate 101a of tortuous ways 10 and the hithermost guide plate 101b of adjacent tortuous ways 10 and guide plate 101c all partly overlap in the projection of horizontal plane.The tortuous guide plate 101d of ways 10 and the hithermost guide plate 101e of adjacent tortuous ways 10 and guide plate 101f also partly overlap in the projection of horizontal plane.Bubble 3 is in passage 2, along guide plate 101d lower surface, rise to after end vertically up, arrive position guide plate 101b lower surface slightly up in adjacent tortuous ways 10, and continue upwards to climb along this lower surface, thereby alternately along the guide plate 101 lower surface complications of adjacent two tortuous ways 10, upwards climb.
Fig. 6 is that guide plate is while being arc, the structural representation of the assembly parts of bubbles assembly, in assembly parts, comprise guide plate 101 and mounting block, the assembling mode of the structure of mounting block and assembly parts is with reference to Fig. 2 and Fig. 3, the up path length that guide plate 101 adopts arcs can further extend bubble.
Fig. 7 is that guide plate is while being arc, the structural representation of bubbles assembly, by after 180 ° of assembly parts upsets in Fig. 6 with unturned assembly parts stack, guide plate 101 is contacted, obtain bubbles assembly 1, comprise tortuous ways 10 and the tortuous passage 2 rising, bubble alternately upwards climbs along the guide plate 101 lower surface complications of adjacent two tortuous ways 10.
In the bubbles assembly 1 of Fig. 1, Fig. 5 and Fig. 7, guide plate 101 on tortuous ways 10 adopts the mode of connection of contact, be that guide plate 101 is independent detachable, thereby can adopt the assembling mode of guide plate 101 along continuous straight runs spread configurations fixing rear stack, realize modularization assembling, assembling mode flexibly, conveniently.Simultaneously, guide plate 101 in the horizontal direction equal intervals is arranged, be parallel to each other, in improving unit volume in the dense degree and gas-to-liquid contact area of guide plate 101, guide plate spacing close on adjacent tortuous ways is less, projection lap area on horizontal plane is larger, and the up path of bubble on guide plate lower surface is longer, and gas-to-liquid contact area is also larger.
Fig. 8 is the bubbles unit construction schematic diagram that adopts another kind of assembling mode, and Fig. 9 is the partial enlarged drawing A of Fig. 8.In this bubbles assembly 1, guide plate 101 relative level face tilts arrange, a plurality of guide plate 101 in the vertical directions adopt the mode being connected as one successively to form tortuous ways 10, in same tortuous ways 10, the vergence direction of the guide plate 101 relative level faces of arbitrary neighborhood is contrary, a plurality of tortuous ways 10 of spaced set, fixing through mounting block in the horizontal direction.
Mounting block comprises and is arranged on the card article 114 at tortuous ways 10 two ends and has the horizontal stripe 115 with the friction tight draw-in groove 1150 of card article 114, and a plurality of tortuous ways 10 are fixed by horizontal stripe 115, obtain bubbles assembly 1.
Figure 10 is the structural representation of tortuous ways 10 in the bubbles assembly 1 of Fig. 8, there is shown the position relationship of adjacent tortuous ways 10, be between the tortuous ways 10 of arbitrary neighborhood, to form the tortuous passage rising, arbitrary guide plate 101 of tortuous ways 10 is overlapping in the projection section of horizontal plane with the hithermost guide plate 101 of adjacent tortuous ways 10, bubble is alternately upwards climbed along the guide plate 101 lower surface complications of adjacent two tortuous ways 10, bubbles member 10 upper and lower ends are respectively arranged with card article 114 simultaneously, convenient fixing.
Bubbles assembly in Fig. 81 adopts first guide plate 101 to be fixedly connected sequentially and is integrated or is that guide plate is from aggregates by the processing mode of a plurality of guide plate 101 integral one-step moldings, obtain after tortuous ways 10, again by the fixing assembling mode of a plurality of tortuous ways 10 combination, facilitate the making of tortuous ways 10, and assembling mode is flexible.Simultaneously, guide plate 101 in the horizontal direction spacing equates, be parallel to each other, can improve dense degree and the gas-to-liquid contact area of guide plate 101 in unit volume, and adjacent tortuous ways spacing is less, the projection lap area of guide plate on horizontal plane is larger, and the up path of bubble on guide plate lower surface is longer, and gas-to-liquid contact area is also larger.
In the bubbles assembly of Fig. 5 and Fig. 8, by mounting block, guide plate and tortuous ways are fixed, mounting block and fixed form are varied, guide plate and tortuous ways are fixed, do not affected flowing of the tortuous up travel path of bubble and nutrient solution.
Guide plate in bubbles assembly is the impenetrable thin plate of bubble, and material is that plastics, metal or glass or other are insoluble to the material of nutrient solution, while needing illumination in microbial cultivation process, is preferably light-passing board.Meanwhile, power or bubble gait of march that bubble is subject to straight up in nutrient solution are very fast, when momentum is larger, all can promotes bubble and move along guide plate.Thereby the angle of inclination of the concrete shape of guide plate and relative level face is not all subject to strict restriction, bubble can be up and not because interrupted causing stops completely, specifically if the mixing of waviness, arc or plane etc. and several shapes is all feasible, adopting surface is that the guide plate of curved surface is as arc or waviness plate, can further increase bubble travel path length, when guide plate is smooth plate, the angle of itself and horizontal plane is preferably 10～30 °, more preferably 20 °.
It may be noted that, when bubble upwards climbs, guide plate relative level face tilt degree is little, and the movement rate of bubble is slow, gas-liquid stirring degree is low, but gas-liquid contact time is long, path length or the gas-to-liquid contact area of bubble operation are larger, and inclined degree is when larger, the movement rate of bubble is very fast, gas-liquid stirring degree is large, and gas-liquid contact time is short, and total path or the gas-to-liquid contact area of bubble operation are less.
The concrete operations of microorganism culturing also can comprise substratum preparation and the pre-treatment such as conventional sterilizing that nutrient solution is carried out, and the operations such as separating-purifying after having cultivated.
The specific embodiment of a microorganism culturing is provided below, and this microbial culture method comprises the following steps:
Treat that culturing micro-organisms is micro-algae Synechococcussp.MA19, the compound method of substratum BG11 is: by SODIUMNITRATE 1.5g, and dipotassium hydrogen phosphate 0.04g, sal epsom (MgSO
47H
2o) 0.075g, calcium chloride (CaCl
22H
2o) 0.036g, citric acid 0.006g, ferric ammonium citrate 0.006g, EDTA0.001g, sodium carbonate 0.02g, trace metal solution 1.0ml, mixed dissolution is in 1L distilled water, and regulating pH is 7.1, puts into refrigerator stand-by after sterilizing.Wherein the collocation method of trace metal solution is: by boric acid 2.86g, and Manganous chloride tetrahydrate (MnCl
24H
2o) 1.81g, zinc sulfate (ZnSO
47H
2o) 0.222g, Sodium orthomolybdate (NaMoO
42H
2o) 0.39g, copper sulfate (CuSO
45H
2o) 0.079g, Jing Ti/Bao Pian COBALT NITRATE CRYSTALS/FLAKES (Co (NO
3)
26H
2o) 49.4mg, adds and in 1.0L distilled water, dissolves and get final product.
Micro-algae is introduced to the process of the nutrient solution of incubator and first carried out preculture, preculture comprises the following steps:
S01, in Erlenmeyer flask, the micro-algae liquid of 10ml being mixed with 30ml substratum BG11, at 25 ℃, LED light-illuminating and pass into continuously under the condition of gas and cultivate 24h, obtain micro algae culturing liquid 1, is slightly muddy green liquid;
S02, after 3 days, 35ml micro algae culturing liquid 1 is mixed with 165ml substratum BG11, proceed to cultivate, the same S01 of culture condition, obtains micro algae culturing liquid 2;
S03, after 3 days, the 200ml micro algae culturing liquid 3 that S02 is obtained is divided into three equal parts and is divided in Erlenmeyer flask, adds respectively substratum BG11 to 200ml, continue to cultivate, condition is identical with S01, becomes muddiness to micro algae culturing liquid, obtains micro algae culturing liquid 3;
S04, after 1 day, the micro algae culturing liquid 3 in three Erlenmeyer flasks is merged in an Erlenmeyer flask, add substratum BG11 to 2L, continue to cultivate, the same S01 of culture condition, obtains green muddy micro-algae preculture liquid.
Micro-algae preculture liquid that preculture is obtained adds in the container of high-density microbial cultivation device, then adds 18L substratum BG11, opens the LED lamp in high-density microbial cultivation device, passes into continuously gas simultaneously, cultivates.
The preculture of micro-algae and the gas in culturing process are for containing carbonic acid gas 15%(volume fraction), 100ppm nitrogen peroxide, the air of 250ppm sulfurous gas, in culturing process, air input is 1 cubic metre of per minute.
Microbial culture method of the present invention also can be used for culturing bacterium, when culturing bacterium is E.coli, the compound method of substratum LB is: by Tryptones (tryptone) 10g, yeast extract (yeastextract) 5g, sodium-chlor (NaCl) 10g, agar powder 15-20g, adds in 1000mL distilled water and dissolves, with 5mol/LNaOH solution, about 0.2ml adjusts pH to 7.2 and get final product, stand-by after 121 ℃ of sterilizing 30min.Will treat culturing bacterium E.coli introduce in the substratum LB in culture vessel, cultivate after passing into gas.
Figure 11 is the cross-sectional view of high-density microbial cultivation device, Figure 12 is the STRUCTURE DECOMPOSITION schematic diagram of high-density microbial cultivation device, Figure 13 is the stereographic map of high-density microbial cultivation device, high-density microbial cultivation device comprises container and is arranged on bubbles assembly 1, the bubble generator 6 in container, also comprises gas leading-in device.
Container is specially incubator, comprises casing 41, case lid 42 and production well 43, and casing 41 bottom surfaces are about 1 * 1m
2, high about 1m, the nutrient solution liquid level in incubator is higher than bubbles assembly 1, and casing 41 and case lid 42 are made by plastics or metal or glass or other material respectively, without particular restriction.
Gas leading-in device comprises gas distribution device 51 and intake ducting 52, gas imports after the air chamber of container bottom through intake ducting 52, through bubble generator 6, in nutrient solution, produce the bubble that diameter is less, bottom by bubbles assembly 1 enters, with containing bubble 3 after the nutrient solution generation exchange of substance of culturing micro-organisms, overflow nutrient solution after by production well 43, discharged.Bubble generator 6 is microwell plate, and the packing ring 7 of sealing use is set between air chamber and bubble generator.It can be also that microporous pipe or micro-hole aerator or other can form gas the device of micro-bubble in nutrient solution that bubble is sent out generating unit.
The structure of bubbles assembly 1 and assembling mode are referring to Fig. 1～Fig. 5, comprise tortuous ways and mounting block, by the equidistant spread configuration of guide plate along continuous straight runs of same size fixing, obtain assembly parts, assembly parts stack by the assembly parts that overturn after 180 ° with not rotation, guide plate contact on tortuous ways is connected, obtains the bubbles assembly that a plurality of tortuous ways form.Concrete, guide plate is long 1 meter, wide 10.6cm, the square transparent plate of thick 1mm, with the angle of horizontal plane be 20 degree, 28 (in figure, only showing a part) forms tortuous ways at the vertical direction guide plate connecting that contacts up and down successively, equal intervals (20mm) is arranged 45 (only showing a part in figure) tortuous ways in the horizontal direction, after fixing, obtain bubbles assembly, bubble alternately upwards climbs along the guide plate lower surface complications of adjacent two tortuous ways, until overflow containing the nutrient solution for the treatment of culturing micro-organisms.
After bubbles assembly 1 is arranged in casing 41, between bubbles assembly 1 and the inwall of casing 41, leave space 8, for the nutrient solution containing treating culturing micro-organisms that is subject to bubble agitation, flow, promote the material balance in culture system to distribute, improve culture efficiency, specifically in culturing process, when bubble is advanced along the tortuous path making progress in the passage of bubbles assembly 1, containing treat that the nutrient solution of culturing micro-organisms also moves upward thereupon, then capable with bubble, go out the rear space 8 between casing 41 and bubbles assembly 1 of bubble guidance set 1 and be back to casing 41 bottoms.
In this incubator, mounted LED lamp on the guide plate of bubbles assembly 1, project light onto around bubble, make to treat that culturing micro-organisms can obtain carries out the essential carbonic acid gas of photosynthesis and luminous energy simultaneously, make to treat that culturing micro-organisms can carry out photosynthesis and growth rapidly effectively.
In the bubbles assembly 1 of the present embodiment, bubble is containing treating that the travel path length in the nutrient solution of culturing micro-organisms is 2.99m, and relatively vertically travel distance (1m) improves closely 200%, and bubble 3 is 134m with the contact area of nutrient solution containing micro-algae
2, than its direct area 1m when vertical direction is advanced
2increase by 134 times, significantly improved the gas-to-liquid contact area in unit volume, promote the culturing process of gas-liquid exchange of substance and microorganism.Meanwhile, in the process that bubble is upwards advanced in complications, drive the nutrient solution containing micro-algae to flow, improve gas-liquid agitation frequency, promote material balance, improve microorganism culturing efficiency.Along with treating the continuous merisis of culturing micro-organisms, the light transmission of nutrient solution sharply reduces, and microorganism is main only effectively to grow in the region that can receive light, and the superiority of this device just fully demonstrates out.
In micro-algae culturing process, by observing, green change and detect the growing state that nutrient solution optical density(OD) changes to weigh micro-algae, test-results shows, adopt this device to carry out micro-algae cultivation, per minute can be processed 297 grams of carbonic acid gas, 0.26 gram of nitrogen peroxide, 0.66 gram of sulfurous gas, and produce 162 grams of micro-algal biomass, culture efficiency significantly improves.
When this high-density microbial cultivation device is applied to off gas treatment, need in nutrient solution, introduce corresponding microorganism, as process carbonic acid gas introduce using carbonic acid gas as the microorganism of carbon source, process sulfur oxide introduce can metabolism sulfur oxide microorganism, process nitrogen oxide introduce can the microorganism of metabolism nitrogen oxide in, the bubble generator that during processing, waste gas is imported to culture apparatus forms bubble in nutrient solution, for microorganism provides gas nutrition.
Above content is in conjunction with concrete preferred implementation further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, can also make some simple deduction or replace, all should be considered as belonging to protection scope of the present invention.
Claims (10)
1. a bubbles assembly, is characterized in that: it comprises the tortuous ways that at least two along continuous straight runs are arranged; Described tortuous ways comprises that at least two at vertical direction successively connected guide plate up and down, described guide plate relative level face tilt arranges, and the vergence direction of the guide plate relative level face of arbitrary neighborhood is contrary in same tortuous ways, between the tortuous ways of arbitrary neighborhood, form the tortuous passage rising; Arbitrary guide plate of described tortuous ways and the hithermost guide plate of adjacent tortuous ways are overlapping in the projection section of horizontal plane.
2. bubbles assembly according to claim 1, is characterized in that: it also comprises the mounting block for fixing described tortuous ways.
3. bubbles assembly according to claim 2, is characterized in that: described guide plate is smooth plate or arc or waviness plate.
4. bubbles assembly according to claim 3, is characterized in that: described guide plate is smooth plate, and the angle of itself and horizontal plane is 10～30 °.
5. according to claim 1～4 any one institute bubbles assembly, it is characterized in that: adjacent described tortuous ways be spaced apart 15-50mm.
6. a high-density microbial cultivation device, it is characterized in that: described high-density microbial cultivation device comprises container, bubble generator, at least one bubbles assembly as described in claim 1～5 any one, described bubble generator and bubble guidance set are arranged in described container, and described bubble generator is arranged on the bottom of bubbles assembly.
7. according to claim 6 high-density microbial cultivation device, it is characterized in that: between described bubbles assembly and the inwall of described container, leave for the mobile gap of nutrient solution.
8. according to the high-density microbial cultivation device described in claim 6～7, it is characterized in that: on described tortuous ways, be provided with light source.
9. the application of the high-density microbial cultivation device described in claim 6～8 any one in off gas treatment, the method of described off gas treatment comprises: in the container of high-density microbial cultivation device, add nutrient solution and introduce to comprise and using the microorganism of carbonic acid gas as carbon source, microorganism that can metabolism sulfur oxide, at least one microorganism in can the microorganism of metabolism nitrogen oxide is cultivated, and described waste gas is imported to described bubble generator in described nutrient solution, form bubble, bubble enters described passage and climbs along the lower surface of guide plate from the bottom of described bubbles assembly, for microorganism provides gas nutrition.
10. the application of high-density microbial cultivation device claimed in claim 8 in micro-algae is cultivated, be included in the container of high-density microbial cultivation device and add nutrient solution and introduce micro-algae and cultivate, and carbonated gas is imported to described bubble generator in described nutrient solution, form bubble, bubble enters described passage and climbs along the lower surface of guide plate from the bottom of described bubbles assembly, for microorganism provides gas nutrition, light source described in culturing process provides luminous energy for micro-algae.
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| WO2015042950A1 (en) * | 2013-09-30 | 2015-04-02 | 钟琦 | Bubble-guiding assembly, device for culturing high-density microorganisms and use thereof |
| CN105985910A (en) * | 2015-03-05 | 2016-10-05 | 华东理工大学 | Novel method and process for carbon supplement during microalgae culture |
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Application publication date: 20140122 |