CN101735941A - Continuous-flow and ultrasonic-wave light biological hydrogen producing reactor - Google Patents

Continuous-flow and ultrasonic-wave light biological hydrogen producing reactor Download PDF

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Publication number
CN101735941A
CN101735941A CN 200910250851 CN200910250851A CN101735941A CN 101735941 A CN101735941 A CN 101735941A CN 200910250851 CN200910250851 CN 200910250851 CN 200910250851 A CN200910250851 A CN 200910250851A CN 101735941 A CN101735941 A CN 101735941A
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ultrasonic
continuous
hydrogen producing
flow
wave light
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CN101735941B (en
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朱恂
谢学旺
王永忠
廖强
王宏
丁玉栋
李俊
叶丁丁
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Chongqing University
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Chongqing University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/04—Bioreactors or fermenters specially adapted for specific uses for producing gas, e.g. biogas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli

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  • Life Sciences & Earth Sciences (AREA)
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Abstract

The invention discloses a continuous-flow and ultrasonic-wave light biological hydrogen producing reactor comprising a light source, a transparent tank irradiated by the light source and an ultrasonic cell crusher, wherein one side of the transparent tank is provided with a liquid inlet, the other side is provided with a liquid outlet, and the top of the transparent tank is provided with an air inlet and an air outlet; an amplitude transformer of the ultrasonic cell crusher vertically and downwards extends into a bacterium suspension filled in the transparent tank, and air sealing is arranged between the amplitude transformer and an upper cover of the transparent tank. The continuous-flow and ultrasonic-wave light biological hydrogen producing reactor combines a flat plate type reactor and the ultrasonic cell crusher so as to enhance organic matter as a carbon source in a degraded solution and raise the hydrogen producing speed of photosynthetic bacteria; when low-strength ultrasonic waves propagate in a medium, the continuous-flow and ultrasonic-wave light biological hydrogen producing reactor can change the permeability of a cell membrane, reinforce matter delivery, increase the metabolic activities of bacteria and promote the generation of useful matter; the continuous-flow and ultrasonic-wave light biological hydrogen producing reactor enhances the speed of a reactant entering the active position of enzyme or cells, promotes the mass transfer diffusion process that a product departs from the active center of the enzyme and enters a liquid medium, raises the enzyme activity, promotes the catalytic reaction of the enzyme and raises the speed of biological reaction.

Description

Continuous-flow and ultrasonic-wave light biological hydrogen producing reactor
Technical field
The present invention relates to a kind of light biological hydrogen producing reactor, relate in particular to a kind of continuous-flow and ultrasonic-wave light biological hydrogen producing reactor.
Background technology
Utilize photosynthetic bacteria hydrogen production to be considered to biological hydrogen production one of the most promising mode (DebabrataDas, et al.Hydrogen production by biological processes:a survey ofliterature.International Journal of Hydrogen Energy 26 (2001) 13-28.).This mainly be because: hydrogen production with photosynthetic bacteria has higher photochemical transformation rate in theory; It can avoid the problem of oxygen quenching; This method has the ability of utilization than wide spectral range; This method has the ability of the organic pollutant that removal produced in wastewater treatment process.
In the prior art, light biological hydrogen producing reactor have tubular type, flat, stir three types of pot types.Flat bioreactor dimming capability is very strong, can guarantee that effective liquid layer fully is subjected to light, strengthens luminous energy and absorbs conversion.But above-mentioned three types all exist bacteria suspension inner cell film thicker, and the product hydrogen in the cytolemma is difficult for passing in cytolemma, and the substrate in the bacteria suspension is difficult for entering in the cytolemma, therefore, have the not high problem of hydrogen rate of producing always.
Summary of the invention
At the prior art above shortcomings, it is strong to the invention provides a kind of dimming capability, effectively liquid layer is subjected to light abundant, and the metabolism of photosynthetic bacterium, growth and breeding ability are strong, can handle organic waste water continuously and produce the high continuous-flow and ultrasonic-wave light biological hydrogen producing reactor of hydrogen rate.
The continuous-flow and ultrasonic-wave light biological hydrogen producing reactor that the present invention improves comprises the transparent casing and the ultrasonic cell disruptor of light source, light source irradiation; One side of described transparent casing is provided with fluid inlet, and opposite side is provided with liquid outlet, and the top is provided with inlet mouth and air outlet; The horn of described ultrasonic cell disruptor puts in the bacteria suspension that transparent casing packs into straight down, hermetic seal between the loam cake of described horn and transparent casing.
Further, described horn is inserted the interior 2cm～3cm of bacteria suspension that transparent casing is packed into.
Beneficial effect of the present invention: continuous-flow and ultrasonic-wave light biological hydrogen producing reactor compared with prior art has following advantage:
1, the present invention combines flat-plate reactor and ultrasonic cell disruptor, be intended to improve the organism in the degraded solutions and improve the hydrogen-producing speed of photosynthetic bacterium, when low-intensity ultrasonic is propagated in medium, can change the permeability of cytolemma, strengthen mass transport, thereby increase the generation of the metabolic activity and the promotion benefit materials of cell.Simultaneously, ultrasonic wave is in communication process, can attenuate mass transfer frictional belt and add the motion of fast solute particle, improve reactant and enter enzyme or cytoactive position, promote that resultant leaves the mass transfer diffusion process that enzyme active center enters liquid medium, thereby the raising enzymic activity, the speed of promotion enzymic catalytic reaction, raising biological respinse.In addition, suitable low-intensity ultrasonic has improved the permeability of cytolemma and cell walls when acting on bacterium, thereby improves the metabolic function of bacterium, has promoted the growth of bacterium.
2, the present invention adopts the low intensity ultrasound wave technology to improve the metabolic function of bacterium, promoted the growth of bacterium, increased the ability of thalli growth breeding, reduced the losing issue of bacterial classification under the bacterial suspension training method, strengthen photosynthetic bacterium and handled the ability of organic waste water continuously, improved the product hydrogen performance of photosynthetic bacterium.
3, to have a cost low for continuous-flow and ultrasonic-wave light biological hydrogen producing reactor of the present invention, easy to operate, be convenient to clean, the reactor floor space is little, unit volume is produced hydrogen rate advantages of higher, therefore continuous-flow and ultrasonic-wave light biological hydrogen producing reactor of the present invention can be widely used in industries such as environmental protection, the energy, food, feed, has a good application prospect.
Description of drawings
Accompanying drawing is a structural representation of the present invention.
The structure of the Reference numeral representative in the accompanying drawing is as follows:
The transparent casing 3-of 1-light source 2-fluid inlet 4-goes out mouth 5-inlet mouth 6-air outlet 7-ultrasonic cell disruptor 8-horn 9-loam cake 10-main control system 11-transverter at night
Embodiment
Below in conjunction with the drawings and specific embodiments the present invention is done explanation in further detail.
Accompanying drawing is a structural representation of the present invention, and as shown in the figure: continuous-flow and ultrasonic-wave light biological hydrogen producing reactor comprises the transparent casing 2 and the ultrasonic cell disruptor 7 of light source 1, light source 1 irradiation.The bottom of transparent casing 2 is provided with fluid inlet 3, and the opposite side relative with fluid inlet 3 is provided with liquid outlet 4, and the height of liquid outlet 4 is higher than fluid inlet 3, and the loam cake 9 at the top of transparent casing 2 is provided with inlet mouth 5 and air outlet 6.Ultrasonic cell disruptor 7 comprises main control system 10, transverter 11 and horn 8, because of the structure of ultrasonic cell disruptor 7 is a prior art, so this tired not stating.The bottom of horn 8 puts in the transparent casing 2 straight down and inserts the interior 2cm～3cm of bacteria suspension that transparent casing 2 is packed into.Seal by sealing-ring and seal gum between the open holes on horn 8 and the loam cake 9.
When using this continuous-flow and ultrasonic-wave light biological hydrogen producing reactor to carry out hydrogen manufacturing, at first bacteria suspension is added in the transparent casing 2 by fluid inlet 3, can add nutritive substances such as organic carbon source, nitrogen, phosphorus, potassium in bacteria suspension, liquid outlet 4 is connected with Hydrogen Separation equipment; Add argon gas or other rare gas elementes from inlet mouth 5,, mainly make bacteria suspension be in anaerobic state the 6 displacements discharge of the air in the transparent casing 2 from the air outlet; Open light source 1, light source is radiated on the bacteria suspension by transparent casing 2; Restart main control system 10, regulate the output rating of main control system 10, spreading out of low-intensity ultrasonic in the horn 8 makes bacteria suspension produce vibration, low-intensity ultrasonic ultrasonic radiation thalline, metabolism and the thalli growth of photosynthetic bacterium in the reactor have been improved, strengthened substrate and be delivered to the speed of microorganism, and bacteria suspension and organic nutrient solution for cultivating are flowed out along the fixed runner, prolonged the treatment time of ultrasonic wave reaction solution.Isolate hydrogen and raffinate by the external hydrogen separating device, the raffinate reusable edible adds in the reactor by fluid inlet once more.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement technical scheme of the present invention, and not breaking away from the aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.

Claims (2)

1. continuous-flow and ultrasonic-wave light biological hydrogen producing reactor, the transparent casing (2) that comprises the irradiation of light source (1) and light source (1), one side of described transparent casing (2) is provided with fluid inlet (3), and opposite side is provided with liquid outlet (4), and the top is provided with inlet mouth (5) and air outlet (6); It is characterized in that: also comprise ultrasonic cell disruptor (7), the horn (8) of described ultrasonic cell disruptor (7) puts in the bacteria suspension that transparent casing (2) packs into straight down, hermetic seal between the loam cake (9) of described horn (8) and transparent casing (2).
2. continuous-flow and ultrasonic-wave light biological hydrogen producing reactor according to claim 1 is characterized in that: described horn (8) is inserted the interior 2cm～3cm of bacteria suspension that transparent casing (2) is packed into.
CN 200910250851 2009-12-30 2009-12-30 Continuous-flow and ultrasonic-wave light biological hydrogen producing reactor Expired - Fee Related CN101735941B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102304468A (en) * 2011-08-12 2012-01-04 河南农业大学 Device for enhancing hydrogen production activity of photosynthetic bacteria
CN102358617A (en) * 2011-06-29 2012-02-22 西安交通大学 Methanol hydrogen production apparatus, and method for preparing hydrogen by using the same
CN105861566A (en) * 2016-05-18 2016-08-17 东北农业大学 Method and device for producing hydrogen through pulsed power ultrasonic enhanced anaerobic biological fermentation
CN104944598B (en) * 2015-06-20 2018-04-06 北京泰昌科睿环保科技有限公司 A kind of photosynthetic bacteria sewage water treatment method

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5104803A (en) * 1988-03-03 1992-04-14 Martek Corporation Photobioreactor
CN100587054C (en) * 2006-05-15 2010-02-03 华东理工大学 Novel multi-section flat type photo-bioreactor
CN101020889B (en) * 2007-03-12 2010-12-01 重庆大学 Micro slot transmitting plate type photobilogical hydrogen producing reactor

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102358617A (en) * 2011-06-29 2012-02-22 西安交通大学 Methanol hydrogen production apparatus, and method for preparing hydrogen by using the same
CN102358617B (en) * 2011-06-29 2013-07-10 西安交通大学 Methanol hydrogen production apparatus, and method for preparing hydrogen by using the same
CN102304468A (en) * 2011-08-12 2012-01-04 河南农业大学 Device for enhancing hydrogen production activity of photosynthetic bacteria
CN104944598B (en) * 2015-06-20 2018-04-06 北京泰昌科睿环保科技有限公司 A kind of photosynthetic bacteria sewage water treatment method
CN105861566A (en) * 2016-05-18 2016-08-17 东北农业大学 Method and device for producing hydrogen through pulsed power ultrasonic enhanced anaerobic biological fermentation

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