CN101020889A - Micro slot transmitting plate type photobilogical hydrogen producing reactor - Google Patents
Micro slot transmitting plate type photobilogical hydrogen producing reactor Download PDFInfo
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- CN101020889A CN101020889A CNA2007100782742A CN200710078274A CN101020889A CN 101020889 A CN101020889 A CN 101020889A CN A2007100782742 A CNA2007100782742 A CN A2007100782742A CN 200710078274 A CN200710078274 A CN 200710078274A CN 101020889 A CN101020889 A CN 101020889A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/04—Bioreactors or fermenters specially adapted for specific uses for producing gas, e.g. biogas
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Abstract
The micro slot transmitting plate type photobiological hydrogen producing reactor consists of one reactor box with at least one liquid inlet for adding bacterial suspension, organic nutritious liquid or organic waste water, and at least one liquid outlet connected to outer pipeline for exhausting hydrogen and residual liquid; and one transmitting plate covering the upper end of the reactor box. It features that the transmitting plate with homogeneously distributed slots in the inner surface introduces light into the reactor and doubles as the microbe attaching solid for microbe to growth to form biological membrane. The reactor box and the transmitting plate are made of organic glass or transparent resin. The present invention has the features of low cost, convenient operation, high light regulating capacity and homogeneous light distribution, and may be used in environment protection, energy source and other industry.
Description
Technical field
The present invention relates to biological hydrogen production reactor, be specifically related to utilize photosynthetic bacterium to realize the micro slot transmitting plate type photobilogical hydrogen producing reactor of biological hydrogen production.
Background technology
Utilize photosynthetic bacteria hydrogen production to be considered to biological hydrogen production one of the most promising mode (Debabrata Das, et al.Hydrogen production by biologicalprocesses:a survey of literature. Internat ional Journal ofHydrogen Energy 26 (2001) 13-28.).Photosynthetic bacterium can utilize small organic molecule to produce hydrogen, can handle organic waste comparatively completely, only needs the operating environment of normal temperature and pressure again, possess the prospect of accomplishing scale production, so environmental benefit is more remarkable, in the unique advantage in biological hydrogen production field.Traditional view thinks that the intravital system for producing hydrogen of microorganism (mainly being enzyme) just can keep stable behind the realization cell fixation, thereby is beneficial to the product hydrogen of realizing the sustained continuous stream mode.So reactor design often need be considered the cell fixation scheme.Up to now, adopt the immobilization technology of pure strain in the biological hydrogen production research mostly.Biological membrane type reactor is simple to operate, the fixing condition gentleness, and the cytoactive loss is little, and carrier can use repeatedly.It is not firm that shortcoming is that microorganism combines with carrier, easily comes off.Carrier immobilized technology pair cell itself does not have special requirement, and the subject matter that needs to consider is to select aspects such as suitable fixation support material and immobilization means.The essential condition of photosynthetic hydrogen production only, illumination has improved nitrogenase activity and ATP energy level, and the hydrogen production with photosynthetic bacteria ability is improved.Guaranteeing enough intensities of illumination in the reactor, improve the photochemical transformation rate of hydrogen production with photosynthetic bacteria process, is the another technical essential of reactor design.
At present, existing light biological hydrogen producing reactor has tubular type, board-like, stir three types of pot types, wherein, the system sealing of tubular type bioreactor is good, be connected with miscellaneous equipment easily, but for the light-receiving area that improves the reactor unit volume and the intensity of illumination in the reactor, the caliber of reactor is (Murtuza A.Syed too greatly, Paul F.Henshaw.Effect of tube sizeon performance of a fixed-film tubular bioreactor for conversionof hydrogen sulfide to elemental sulfur.Water Research 37 (2003) 1932-1938.), yet adopt the size and the manufacturing process difficulty of little caliber meeting augmenting response device, increase tooling cost, simultaneously cause reactor plugs easily, cause reactor efficiency to reduce, even lost efficacy; Flat bioreactor dimming capability is very strong, can guarantee that effective liquid layer fully is subjected to light, strengthen luminous energy and absorb conversion, but Continuous Flow or stream that existing flat bioreactor is generally microbial suspension add the type reactor, the cell volume of holding in the reactor is less, in order to guarantee transparence enough in the suspension, channel size can not be excessive, so the product hydrogen rate of unit reactor floor space is not high.Stirring the pot type photoreactor is on the basis of traditional microorganism fermentation tank, increasing illumination is used for the photosynthetic bacterium cultivation and produces hydrogen, its advantage is that higher volume of culture is arranged in limited floor space, shortcoming is that light will could arrive microorganism by liquid, light intensity attenuation is big, and the photochemistry efficiency of conversion is low, stirs the shearing force meeting cell growth inhibiting and the damaging cells that produce, the mode of cell suspension culture also can't realize the product hydrogen technology of Continuous Flow in addition, also is mixed with the losing issue of bacterial classification simultaneously.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of extinction ability strong, and photochemical transformation efficient height is easy to microorganism in the wall biofilm, utilizes photosynthetic bacterium to realize the micro slot transmitting plate type photobilogical hydrogen producing reactor of biological hydrogen production.
In order to solve the problems of the technologies described above, a technical scheme of the present invention is, micro slot transmitting plate type photobilogical hydrogen producing reactor is by light-passing board, the reactor casing, at least one liquid inlet, at least one liquid outlet constitutes, described liquid inlet and liquid outlet are opened respectively on the reactor casing, liquid inlet is used to add bacteria suspension or organic nutrient solution for cultivating or organic waste water, liquid outlet is used for being connected with exterior line, get rid of hydrogen and raffinate, light-passing board covers the top at the reactor casing, wherein: the internal surface of light-passing board is provided with equally distributed conduit, light-passing board imports light in the reactor, simultaneously again as the solid attaching substratum of microorganism, microorganism growth and breeding in conduit forms microbial film, and incident light directly acts on the organic produce hydrogen of microbiological deterioration in the microbial film by light-passing board; The degree of depth of described conduit is not more than 2mm, and the width of conduit is not more than 2mm, by driving microflute on the reactor light-passing board, has increased the solid substrate surface-area of supplying with photosynthetic bacterium absorption on the one hand, helps it and adsorbs on light-passing board and breed; On the other hand, the structure of micro-channel is beneficial to established microbial film from the free photosynthetic bacterium of liquid phase main body absorption, quickens the speed of cell fixation; Microbial film can also be linked to be tridimensional network along the conduit that rises and falls simultaneously, effectively resists the hydraulic shear effect under the Continuous Flow operating mode; And the channel structure that rises and falls has increased the disturbance of convection cell, adds the expansion of biomembrane area, is beneficial to the transmission in reactor of substrate and product; Described light-passing board is made with synthetic glass or transparent resin, is convenient to light biomembranous transmission in reactor, increases the intensity of illumination that arrives microbial film place light in the reactor.
In the preferred version of the present invention, in described reactor casing and near the position of liquid inlet, be horizontally disposed with flute type pipe, described flute type pipe has inner chamber, and it is sealed at both ends, the upper surface and the base plate of described flute type pipe and reactor casing all leave the gap, middle part at described flute type pipe has inlet hole, inlet hole is relative with liquid inlet, described inlet hole is communicated with liquid inlet by pipe connecting, both side surface at flute type pipe inlet hole is evenly distributed with at least two outlet openings, outlet opening is relative with the wall that liquid inlet is set, and by flute type pipe is set, realizes the purpose of liquid uniformly distributing.
In the preferred version of the present invention, base plate near liquid inlet in the described reactor casing is provided with and the vertical current-sharing grid of base plate, the current-sharing grid is separated into two Room with the reactor casing, on the current-sharing grid, be evenly distributed with through hole, by the current-sharing grid is set, realize the purpose of liquid uniformly distributing.
In the preferred version of the present invention, conduit is arranged in parallel, and the spacing between conduit and the conduit is between 1mm ∽ 5mm.
In the preferred version of the present invention, the netted arrangement of conduit, the spacing between the conduit that is arranged in parallel is between 1mm ∽ 5mm.
The beneficial effect of micro slot transmitting plate type photobilogical hydrogen producing reactor of the present invention is, by drive microflute on light-passing board, increased the surface of supplying with photosynthetic bacterium absorption on the one hand, is beneficial to it and adsorbs on light-passing board and breed; On the other hand, the structure of micro-channel is beneficial to established microbial film from the free photosynthetic bacterium of liquid phase main body absorption, quickens the speed of cell fixation; Microbial film can also be linked to be tridimensional network along the conduit that rises and falls simultaneously, effectively resists the hydraulic shear effect under the Continuous Flow operating mode; And the channel structure that rises and falls has increased the disturbance of convection cell, adds the expansion of biomembrane area, is beneficial to the transmission in reactor of substrate and product; This invention simultaneously has that cost is low, easy to operate, dimming capability is strong, and the characteristics that intensity of illumination is evenly distributed can be used for biological membrane type photosynthetic organism hydrogen generation reactor.Therefore light biological hydrogen producing reactor of the present invention can be applicable to industries such as environmental protection, the energy, has a good application prospect.
Description of drawings
The present invention is further detailed explanation below in conjunction with the drawings and specific embodiments:
Fig. 1 is the structural representation that inside of the present invention is provided with the micro slot transmitting plate type photobilogical hydrogen producing reactor of flute type pipe.
Fig. 2 is the structural representation that inside of the present invention is provided with the micro slot transmitting plate type photobilogical hydrogen producing reactor of current-sharing grid.
Fig. 3 is that conduit reticulates structural representation on the light-passing board.
Embodiment
Embodiment one, referring to Fig. 1, be provided with by inside shown in Figure 1 flute type pipe micro slot transmitting plate type photobilogical hydrogen producing reactor structural representation as can be known, a kind of micro slot transmitting plate type photobilogical hydrogen producing reactor of the present invention, constitute the bottom sealing of described reactor casing 3, open topped by light-passing board 2, reactor casing 3, liquid inlet 4, liquid outlet 5; One of them sidewall of reactor casing 3 is provided with a liquid inlet 4, sidewall on liquid inlet 4 opposites is provided with 3 liquid outlets 5, liquid inlet 4 is used to add bacteria suspension or organic nutrient solution for cultivating or organic waste water, liquid outlet 5 is used for being connected with exterior line, get rid of hydrogen and raffinate, the quantity of liquid inlet 4 and liquid outlet 5 also can be 2,4,5,6 even more a plurality of, and effect is to realize the liquid uniformly distributing; Select for use thick synthetic glass of 5～10mm or transparent resin to make light-passing board 2, wherein transparent resin can be selected materials such as transparent polyethylene, polypropylene, nylon, ABS for use, the size of light-passing board 2 should be slightly larger than the overall dimensions of reactor casing 3, one side at light-passing board 2 is provided with equally distributed conduit 1, the degree of depth of conduit 1 is not more than 2mm, the width of conduit 1 is not more than 2mm, described conduit 1 is to be arranged in parallel, spacing between conduit and the conduit is between 1mm ∽ 5mm, and the quantity of described conduit 1 is determined according to the inner cavity size of reactor casing 3; Light-passing board 2 is covered top at reactor casing 3, the inside of the one side of conduit towards reactor casing 3 is wherein arranged on the light-passing board 2, connect light-passing board 2 and reactor casing 3 by fastening piece, fastening piece can be flange or bolt, in order to increase the stopping property between light-passing board 2 and the reactor casing 3, can increase by a circle projection at the internal surface of light-passing board 2, and concave groove is set on projection, the size of groove and shape are corresponding with reactor casing 3, the matrix sealing-ring is embedded in the concave groove of light-passing board 2, again reactor casing 3 is embedded on the matrix sealing-ring, then with flange or bolting.
Embodiment two, and referring to Fig. 4, the structural relation of described micro slot transmitting plate type photobilogical hydrogen producing reactor is with embodiment one, and difference is: the conduit 1 that is provided with on light-passing board 2 adopts netted arrangement, and the spacing between the conduit that is arranged in parallel is between 1mm ∽ 5mm.
Embodiment three, referring to Fig. 1, in order to realize the even mobile purpose of liquid, on the basis of embodiment one or embodiment two, increase flute type pipe 6, described flute type pipe 6 is horizontally set in the reactor casing 3 and the position of close liquid inlet 4, described flute type pipe 6 has inner chamber, and it is sealed at both ends, described flute type pipe 6 is gapped with the upper surface and the base plate current-sharing of reactor casing 3, flute type pipe 6 is separated into two Room with reactor casing 3, middle part at described flute type pipe 6 has inlet hole, inlet hole is relative with liquid inlet 4, and described inlet hole is communicated with liquid inlet 4 by pipe connecting, and described bacteria suspension or organic nutrient solution for cultivating or organic waste water enter the inner chamber of flute type pipe 6 by liquid inlet 4, both side surface in flute type pipe 6 centre inlet holes is evenly distributed with 6 outlet openings, and outlet opening is relative with the wall that liquid inlet 4 is set.The quantity of outlet opening also can be other natural numbers, bacteria suspension or organic nutrient solution for cultivating or organic waste water are by flowing out by the outlet opening that distributes on the flute type pipe 6, return after clashing into the wall of close reactor casing 3, and the gap of the gap by flute type pipe 6 and the top of reactor casing 3 and flute type pipe 6 and base plate flows to another chamber.When being provided with flute type pipe 6, the liquid inlet 4 of reactor casing 3 is one.
Embodiment four, referring to Fig. 2, in order to realize the even mobile purpose of liquid, be with embodiment three differences: on the basis of embodiment one or embodiment two, increase current-sharing grid 7, described current-sharing grid 7 is arranged in the reactor casing 3 near on the base plates of liquid inlet 4 and vertical with base plate, can be by on two corresponding sidewalls of reactor casing 3, draw-in groove being set, the current-sharing grid inserts in the draw-in groove, the current-sharing grid is a kind of porous media board, current-sharing grid 7 is separated into reactor casing 3 two Room that differ in size, on current-sharing grid 7, be evenly distributed with through hole, described bacteria suspension or organic nutrient solution for cultivating or organic waste water enter the cell of reactor casing 3 by liquid inlet 4, flow out to another chamber by the through hole that distributes on the current-sharing grid 7 again.
Yet embodiments of the invention are not limited only to above-mentioned embodiment.
Bacteria suspension or organic nutrient substance are added in the reactor by liquid inlet 4, can in bacteria suspension or organic nutrient substance, add nitrogen, phosphorus, nutritive substances such as potassium, conduit 1 on the light-passing board provides microorganism cells to adhere to solid substrate, microorganism adheres on conduit 1, growth and breeding, form microbial film, generally need 30 days the biofilm phase of 20 ∽, during biofilm, because light-passing board 2 has a lot of bar conduits 1, make microorganism cells have enough attaching surfaces long-pending, thereby the biomembranous surface-area of photosynthetic bacterium in the unit volume reactor and the volume of holding of photosynthetic bacterium have been increased, light passes light-passing board 2 and is passed to the microbial film that is attached in the reactor in the light-passing board conduit 1, improved the degree of uniformity of light distribution in the reactor, strengthened the intensity of illumination that is delivered to biomembranous light, after the film forming, add organic waste water by liquid inlet 4 again, microorganism metabolism organism under the effect of light in the microbial film on the light-passing board 2, a large amount of hydrogen that produce, hydrogen and raffinate be 5 outflows along the fixed runner from liquid outlet, and isolate hydrogen and raffinate by the external hydrogen separating device.This reactor also can adopt batch charging to produce the hydrogen mode, after treating that promptly biofilm is finished, inject organic solution and nutritive medium by liquid inlet, close liquid inlet, collect hydrogen at liquid outlet, treat in the solution organism and nutrition be depleted to a certain amount of after, again from the adding organism and the nutritive substance of liquid inlet batch property.
Claims (5)
1, a kind of micro slot transmitting plate type photobilogical hydrogen producing reactor, constitute by light-passing board (2), reactor casing (3), at least one liquid inlet (4), at least one liquid outlet (5), described liquid inlet (4) and liquid outlet (5) are opened respectively on reactor casing (3), liquid inlet (4) is used to add bacteria suspension or organic nutrient solution for cultivating or organic waste water, liquid outlet (5) is used for being connected with exterior line, get rid of hydrogen and raffinate, light-passing board (2) covers in the upper surface of reactor casing (3), it is characterized in that:
The internal surface of light-passing board (2) is provided with equally distributed conduit (1), and light-passing board (2) imports light in the reactor, and again as the solid attaching substratum of microorganism, microorganism forms microbial film in conduit (1) surface growth breeding simultaneously; The degree of depth of described conduit (1) is not more than 2mm, and the width of conduit (1) is not more than 2mm, and described light-passing board (2) and reactor casing (3) are made with synthetic glass or transparent resin.
2, micro slot transmitting plate type photobilogical hydrogen producing reactor according to claim 1, it is characterized in that: in reactor casing (3) and near the position of liquid inlet (4), be horizontally disposed with flute type pipe (6), described flute type pipe (6) has inner chamber, and it is sealed at both ends, described flute type pipe (6) all leaves the gap with the upper surface and the base plate of reactor casing (3), middle part at described flute type pipe (6) has inlet hole, inlet hole is relative with liquid inlet (4), described inlet hole is communicated with liquid inlet (4) by pipe connecting, both side surface at flute type pipe (6) inlet hole is evenly distributed with at least two outlet openings, and outlet opening is relative with the wall that liquid inlet (4) is set.
3, micro slot transmitting plate type photobilogical hydrogen producing reactor according to claim 1, it is characterized in that: the base plate near liquid inlet (4) in the reactor casing (3) is provided with and the vertical current-sharing grid of base plate (7), current-sharing grid (7) is separated into two Room with reactor casing (3), is evenly distributed with through hole on current-sharing barrier (7).
4, according to claim 1 or 2 or 3 described micro slot transmitting plate type photobilogical hydrogen producing reactors, it is characterized in that: conduit (1) is arranged in parallel, and the spacing between conduit and the conduit is between 1mm ∽ 5mm.
5, according to claim 1 or 2 or 3 described micro slot transmitting plate type photobilogical hydrogen producing reactors, it is characterized in that: the netted arrangement of conduit (1), the spacing between the conduit that is arranged in parallel is between 1mm ∽ 5mm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2007100782742A CN101020889B (en) | 2007-03-12 | 2007-03-12 | Micro slot transmitting plate type photobilogical hydrogen producing reactor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2007100782742A CN101020889B (en) | 2007-03-12 | 2007-03-12 | Micro slot transmitting plate type photobilogical hydrogen producing reactor |
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| CN101020889A true CN101020889A (en) | 2007-08-22 |
| CN101020889B CN101020889B (en) | 2010-12-01 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294132B (en) * | 2008-06-19 | 2011-04-13 | 蔡志武 | Gas push type photobioreactor |
| CN101735941B (en) * | 2009-12-30 | 2012-08-29 | 重庆大学 | Continuous-flow and ultrasonic-wave light biological hydrogen producing reactor |
| CN103114037A (en) * | 2013-01-25 | 2013-05-22 | 河南农业大学 | Biologic membrane photobiologic hydrogen production drip reactor based on light guide carrier |
| CN105255711A (en) * | 2015-11-30 | 2016-01-20 | 华北水利水电大学 | Self-circulation light biological hydrogen production device |
| CN105733924A (en) * | 2014-12-12 | 2016-07-06 | 国家开发投资公司 | Thin-layer horizontal-type photo-biological reactor and photo-biological culture system |
| CN108315253A (en) * | 2018-04-10 | 2018-07-24 | 江西东旺动物微生物有限公司 | A kind of mixed feed Additive Production fermentation system with heat insulation function |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001000168A (en) * | 1999-06-17 | 2001-01-09 | Nippon Telegr & Teleph Corp <Ntt> | Photosynthesis reactor |
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2007
- 2007-03-12 CN CN2007100782742A patent/CN101020889B/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294132B (en) * | 2008-06-19 | 2011-04-13 | 蔡志武 | Gas push type photobioreactor |
| CN101735941B (en) * | 2009-12-30 | 2012-08-29 | 重庆大学 | Continuous-flow and ultrasonic-wave light biological hydrogen producing reactor |
| CN103114037A (en) * | 2013-01-25 | 2013-05-22 | 河南农业大学 | Biologic membrane photobiologic hydrogen production drip reactor based on light guide carrier |
| CN103114037B (en) * | 2013-01-25 | 2015-04-22 | 河南农业大学 | Biologic membrane photobiologic hydrogen production drip reactor based on light guide carrier |
| CN105733924A (en) * | 2014-12-12 | 2016-07-06 | 国家开发投资公司 | Thin-layer horizontal-type photo-biological reactor and photo-biological culture system |
| CN105255711A (en) * | 2015-11-30 | 2016-01-20 | 华北水利水电大学 | Self-circulation light biological hydrogen production device |
| CN108315253A (en) * | 2018-04-10 | 2018-07-24 | 江西东旺动物微生物有限公司 | A kind of mixed feed Additive Production fermentation system with heat insulation function |
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| CN101020889B (en) | 2010-12-01 |
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