CN203613188U - Bubble guide assembly and high-density microbial culture device comprising same - Google Patents
Bubble guide assembly and high-density microbial culture device comprising same Download PDFInfo
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- CN203613188U CN203613188U CN201320614391.7U CN201320614391U CN203613188U CN 203613188 U CN203613188 U CN 203613188U CN 201320614391 U CN201320614391 U CN 201320614391U CN 203613188 U CN203613188 U CN 203613188U
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Abstract
The utility model discloses a bubble guide assembly. The bubble guide assembly comprises at least two zigzag guide components arranged horizontally, wherein each zigzag guide component comprises at least two guide plates vertically and sequentially connected in an up-and-down manner, the guide plates are arranged obliquely relative to the horizontal plane, in addition, oblique directions of any adjacent guide plates in the same zigzag guide component relative to the horizontal plane are opposite, a channel ascending in a zigzag manner is formed between any adjacent zigzag guide components, and projections of any guide plates of the zigzag guide components and the closest guide plates of the adjacent zigzag guide components on the horizontal plane are overlapped partially. According to the bubble guide assembly, the bubble guide area per unit volume is increased greatly, the modular assembly is realized, and the adaptability is high. The utility model further discloses a high-density microbial culture device. The mass production of microbes can be realized by using the device.
Description
Technical field
The utility model relates to microorganism culturing technical field, especially relates to a kind of bubbles assembly and high-density microbial cultivation device thereof.
Background technology
Modern air-polluting major cause is the excessive emissions of burning the industrial gaseous waste of industrial operation generation and the tail gas that the transportation means of multiple oil firing is emitted of gas by burning coal oil firing, its main component comprises harmful carbonic acid gas, nitrogen oxide, sulfur oxide and carbon monoxide and harmless nitrogen and water vapour.In atmospheric layer, having excessive carbonic acid gas can cause Greenhouse effect, and can make seawater souring, is the major cause that causes world environments to worsen.Though nitrogen oxide and sulfur oxide can not cause Greenhouse effect, these gases can become acid after meeting water, and acid rain is exactly to become acid formation after these gases are met water.Acid rain drops to earth's surface, can cause soil with organic matter to run off, impoverishing the soil, and can cause that earth surface water source acidifying makes hydrobiont reduce even disappearance, destroys ecotope.Acid rain is suspended in a kind of reason that still forms haze in the air, can cause serious atmospheric pollution.At present also do not have ripe exhaust gas treatment technology can control the discharge of carbonic acid gas.Developing direction for its treatment technology also the understanding that neither one is unified.It is exactly two kinds of representational study hotspots that seabed is buried with the solid carbon of algae.It is high that technical requirements is buried in seabed, and amount of investment is large.By contrast, the solid carbon of algae has basis widely, and many countries are all carrying out the algae trial of carbon admittedly, method difference, and result is different, but does not also break through so far.
Microorganism ring insurance system is with the reaction of microbial growth substituted chemistry, and allows microorganism that provided artificial environment is be provided to grow.Sulfur oxide, nitrogen oxide and carbonic acid gas in waste gas can naturally water-solublely be all that microorganism growth is used, and microorganism ring insurance system is not only for the absorption of gas but also carry out microorganism culturing.The center of microorganism ring insurance system is biological solid carbon, and the microorganism majority of selecting is various micro-algaes, and foster method for planting can be divided into open and closed.Openly support that to plant be mainly with racing track and pond, its advantage is to build and cost of use is low, be convenient to industrialization, easy cleaning; Its shortcoming is that production efficiency is low, easily contaminated, takes up an area greatly, and water consumption is many.Closed system is mainly various photoreactors, its advantage be production efficiency high, be difficult for contaminated, take up an area little, water consumption is few; Its shortcoming is to build and cost of use is high, the application of easy cleaning, heavy industrialization is comparatively not difficult.As one of emphasis of researching and developing, industry member is looking for one can be used for suitability for industrialized production always, has again high efficiency culture apparatus, but never new breakthrough for many years.The culture systems of existing micro-algae all can not meet the needs of solid carbon, does not also reach the object of micro-algae suitability for industrialized production.
In microbial cultivation process, conventionally Gaseous nutrition thing need to be delivered in the mode of bubble in the nutrient solution of microorganism, especially photosynthetic microorganism is as algae, need to be in the case of existing solar radiation and such as carbonic acid gas etc. to be rich in the gas of carbon, to grow and to carry out photosynthesis in proper nutrition developing medium, thereby increase Gaseous nutrition thing being supplied with in microbial culture medium and be beneficial to microbial growth.At present, the method that increases the supply of Gaseous nutrition thing has three kinds: the firstth, and the transmission rate of increase gas, is pressed into more gas in microorganism culture environment within a certain period of time; The secondth, reduce the diameter of bubble, bubble can be dissolved in nutrient solution; The 3rd is the height that increases the microbial culture medium of bubble experience in incubator.The shortcoming of these three kinds of methods is: 1. all can increase the energy expenditure of microbial cultivation process, thereby increase production cost; 2. the pattern of microbial culture garden can not be amplified to the scale of suitability for industrialized production; 3. can not use the microbial culture garden that only fills a small amount of microbial culture medium to cultivate, limit the application of microbial culture garden; 4. cultivate for micro-algae, prior art can only offer sub-fraction microalgae cell carbon dioxide and luminous energy simultaneously and carry out photosynthesis, has reduced culture efficiency.
Utility model content
The purpose of this utility model is the problem and shortage existing for prior art, provides that a kind of energy consumption is low, efficiency is high, bubbles assembly and the micro-raw culture apparatus of high-density thereof that can modularization assembling.
The technical solution of the utility model is achieved in that a kind of bubbles assembly, comprises the tortuous ways that at least two along continuous straight runs are arranged; Described tortuous ways comprises that at least two at vertical direction successively connected guide plate up and down, described guide plate relative level face tilt arranges, and the vergence direction of the guide plate relative level face of arbitrary neighborhood is contrary in same tortuous ways, between the tortuous ways of arbitrary neighborhood, form the tortuous passage rising; Arbitrary guide plate of described tortuous ways and the hithermost guide plate of adjacent tortuous ways are overlapping in the projection section of horizontal plane.
Described bubbles assembly also comprises the mounting block for fixing described tortuous ways.
Bubbles assembly of the present utility model has greatly increased the area of bubbles in unit volume, and has realized modularization, can assemble easily as required, and the mass-producing that can be applied to microorganism is cultivated.
As further improvement, described guide plate is smooth plate or arc or waviness plate.When described guide plate is smooth plate, the angle of itself and horizontal plane is for being preferably 10～30 °.
As further improvement, adjacent described tortuous ways be spaced apart 15-50mm.
The utility model also provides a kind of high-density microbial cultivation device, described high-density microbial cultivation device comprises container, bubble generator, at least one bubbles assembly as described in claim 1～5 any one, described bubble generator and bubble guidance set are arranged in described container, and described bubble generator is arranged on the bottom of bubbles assembly.
Further, between described bubbles assembly and the inwall of described container, leave for the mobile gap of nutrient solution.Light source, for example LED lamp also can be set on described tortuous ways or on the inwall of container.
The beneficial effects of the utility model are:
The bubbles assembly of the special construction that the utility model provides, greatly improves the area of bubbles, has realized modularization assembling.High-density microbial cultivation device of the present utility model, guide plate in bubbles assembly provides tortuous travel path upwards for the required bubble that carries nutrition gas of microbial cultivation process, make bubble along in the up process of guide plate lower surface, vertical velocity slows down and has increased the motion of horizontal direction, increase exponentially the contact area of the interior bubble of unit volume and microbial culture medium and extended gas-liquid contact time, the required nutritional gas body of microorganism growth can be delivered to microbial culture medium for microorganism growth from gas phase fully, greatly improve material and the energy utilization efficiency in microbial cultivation process, thereby improve culture efficiency and reduced cultivation cost, the complications process of moving upward of bubble has increased gas-liquid agitation frequency simultaneously, drive the motion of microbial culture medium, nutrition required microorganism growth is sent in whole microbial culture medium equably, be beneficial to the culture efficiency that improves microorganism, and design and the assembling mode of bubbles assembly are flexible, can realize modularization, strong adaptability.This high-density microbial cultivation device is applied to off gas treatment and the cultivation of micro-algae, and throughput can reach the level of suitability for industrialized production.
Accompanying drawing explanation
Fig. 1 is the structural representation of the guide plate of tortuous ways bubbles assembly while being multiple;
Fig. 2 is the decomposing schematic representation of guide plate assembly parts of bubbles assembly while being square plate;
Fig. 3 is the structural representation of guide plate assembly parts of bubbles assembly while being square plate;
Fig. 4 is that the guide plate of tortuous ways is the decomposing schematic representation of two assemblies of bubbles when dull and stereotyped;
Fig. 5 is that the guide plate of tortuous ways is two bubbles unit construction schematic diagram when dull and stereotyped;
Fig. 6 is the structural representation of guide plate assembly parts of bubbles assembly while being arc;
Fig. 7 is the bubbles unit construction schematic diagram of the guide plate of tortuous ways while being two arcs;
Fig. 8 is the bubbles unit construction schematic diagram that adopts another kind of assembling mode;
Fig. 9 is the partial enlarged drawing A of Fig. 8;
Figure 10 is the structural representation of tortuous ways in the bubbles assembly of Fig. 8;
Figure 11 is the high-density microbial cultivation device cross-sectional view of the embodiment of the present invention;
Figure 12 is the high-density microbial cultivation device STRUCTURE DECOMPOSITION schematic diagram of the embodiment of the present invention;
Figure 13 is the stereographic map of the high-density microbial cultivation device of the embodiment of the present invention.
Embodiment
Below in conjunction with the drawings and the specific embodiments, the present invention is further described.
Fig. 1～Fig. 5 provides a kind of preferred implementation that facilitates the batch making of guide plate and the assembling of bubbles assembly.As shown in Figure 1, for the structural representation of bubbles assembly, bubbles assembly 1 comprises the tortuous ways 10 that multiple along continuous straight runs are equidistantly arranged, tortuous ways 10 comprises that polylith contacts connected guide plate 101 successively up and down at vertical direction, guide plate 101 relative level face tilts arrange, in same tortuous ways 10, the vergence direction of the guide plate 101 relative level faces of arbitrary neighborhood is contrary, between the tortuous ways 10 of arbitrary neighborhood, form the tortuous passage rising, and arbitrary guide plate 101 of tortuous ways 10 is overlapping in the projection section of horizontal plane with the hithermost guide plate 101 of adjacent tortuous ways 10, bubble climbs from the bottom of bubbles assembly admission passage and along the lower surface of guide plate 101.
In Fig. 1, the assembly method of bubbles assembly 1 is to obtain assembly parts after fixing the guide plate of parallel spaced set in multiple horizontal directions 101, then obtain bubbles assembly 1 in vertical direction stack, specifically, by 180 ° of assembly parts upsets, obtain another assembly parts, by the assembly parts stack before and after upset, guide plate 101 is contacted and relative level face tilt opposite direction, obtain bubbles assembly 1, after stack, can further fix, prevent that guide plate from moving.
As shown in Figure 2, while being square plate for guide plate, the decomposing schematic representation of the assembly parts of bubbles assembly.Assembly parts comprise guide plate 101 and mounting block, mounting block comprises the horizontal stripe 111 and the wedge shape cushion cap 112 that have from the horizontal by the oblique slot 1110 of acute angle, can further comprise fastening piece 113, on wedge shape cushion cap 112, there is the groove 1120 corresponding with horizontal stripe 111 sizes, the thickness of wedge shape cushion cap 112 is vertically highly identical with guide plate 101, and its face close with guide plate 101 is parallel with guide plate 101.
Fig. 3 is that guide plate is while being square plate, the structural representation of the assembly parts of bubbles assembly, horizontal stripe 111 fixed guide plates 101 are used respectively at front and rear at the guide plate 101 of multiple horizontal direction equal intervals setting, in the consecutive position of the guide plate 101 at most end two ends, wedge shape cushion cap 112 is set, make formation passage between the inclined-plane of wedge shape cushion cap 112 and the guide plate of end 101, horizontal stripe 111 two ends are inserted respectively in 1120 on wedge shape cushion cap 112, fix as screw through fastening piece 113, obtain assembly parts.
Fig. 4 is that the guide plate of tortuous ways is two when dull and stereotyped, the decomposing schematic representation of bubbles assembly, and Fig. 5 is the structural representation of corresponding bubbles assembly.This bubbles assembly is obtained by the stack of two assembly parts, and the decomposing schematic representation of assembly parts and structural representation be referring to Fig. 2, Fig. 3, and 180 ° of assembly parts upsets are superimposed upon and on another assembly parts, obtain the bubbles assembly 1 in Fig. 5.Bubbles assembly in Fig. 1 can be obtained by the bubbles assembly stack assembling in multiple Fig. 5.
In bubbles assembly 1 as shown in Figure 5, tortuous ways 10 is made up of two guide plates 101, in adjacent tortuous ways 10, between the tortuous ways 10 of arbitrary neighborhood, form the tortuous passage 2 rising, arbitrary guide plate 101a of tortuous ways 10 and hithermost guide plate 101b and the guide plate 101c of adjacent tortuous ways 10 all partly overlap in the projection of horizontal plane.The tortuous guide plate 101d of ways 10 and hithermost guide plate 101e and the guide plate 101f of adjacent tortuous ways 10 also partly overlap in the projection of horizontal plane.Bubble 3 is in passage 2, rise to after end vertically up along guide plate 101d lower surface, arrive position guide plate 101b lower surface slightly up in adjacent tortuous ways 10, and continue upwards to climb along this lower surface, thereby alternately upwards climb along the guide plate 101 lower surface complications of adjacent two tortuous ways 10.
Fig. 6 is that guide plate is while being arc, the structural representation of the assembly parts of bubbles assembly, in assembly parts, comprise guide plate 101 and mounting block, the assembling mode of the structure of mounting block and assembly parts is with reference to Fig. 2 and Fig. 3, and guide plate 101 adopts arc can further extend the up path length of bubble.
Fig. 7 is that guide plate is while being arc, the structural representation of bubbles assembly, by after 180 ° of assembly parts upsets in Fig. 6 with unturned assembly parts stack, guide plate 101 is contacted, obtain bubbles assembly 1, comprise tortuous ways 10 and the tortuous passage 2 rising, bubble alternately upwards climbs along the guide plate 101 lower surface complications of adjacent two tortuous ways 10.
In the bubbles assembly 1 of Fig. 1, Fig. 5 and Fig. 7, guide plate 101 on tortuous ways 10 adopts the mode of connection of contact, be that guide plate 101 is independent detachable, thereby can adopt the assembling mode of guide plate 101 along continuous straight runs spread configurations fixing rear stack, realize modularization assembling, assembling mode flexibly, conveniently.Simultaneously, guide plate 101 in the horizontal direction equal intervals is arranged, be parallel to each other, in improving the dense degree and gas-to-liquid contact area of guide plate 101 in unit volume, guide plate spacing close on adjacent tortuous ways is less, projection lap area on horizontal plane is larger, and the up path of bubble on guide plate lower surface is longer, and gas-to-liquid contact area is also larger.
Fig. 8 is the bubbles unit construction schematic diagram that adopts another kind of assembling mode, and Fig. 9 is the partial enlarged drawing A of Fig. 8.In this bubbles assembly 1, guide plate 101 relative level face tilts arrange, multiple guide plate 101 in the vertical directions adopt the mode being connected as one successively to form tortuous ways 10, in same tortuous ways 10, the vergence direction of the guide plate 101 relative level faces of arbitrary neighborhood is contrary, the multiple tortuous ways 10 of spaced set in the horizontal direction, fixing through mounting block.
Mounting block comprises and is arranged on the card article 114 at tortuous ways 10 two ends and has and the horizontal stripe 115 of the friction tight draw-in groove 1150 of card article 114, and multiple tortuous ways 10 are fixed by horizontal stripe 115, obtain bubbles assembly 1.
Figure 10 is the structural representation of tortuous ways 10 in the bubbles assembly 1 of Fig. 8, there is shown the position relationship of adjacent tortuous ways 10, be between the tortuous ways 10 of arbitrary neighborhood, to form the tortuous passage rising, arbitrary guide plate 101 of tortuous ways 10 is overlapping in the projection section of horizontal plane with the hithermost guide plate 101 of adjacent tortuous ways 10, bubble is alternately upwards climbed along the guide plate 101 lower surface complications of adjacent two tortuous ways 10, bubbles member 10 upper and lower ends are respectively arranged with card article 114 simultaneously, convenient fixing.
In the bubbles assembly of Fig. 5 and Fig. 8, by mounting block, guide plate and tortuous ways are fixed, mounting block and fixed form are varied, guide plate and tortuous ways are fixed, do not affected flowing of the tortuous up travel path of bubble and nutrient solution.
Guide plate in bubbles assembly is the impenetrable thin plate of bubble, and material is the material that plastics, metal or glass or other are insoluble to nutrient solution, in the time needing illumination in microbial cultivation process, is preferably light-passing board.Meanwhile, it is very fast that bubble is subject to power or bubble gait of march straight up in nutrient solution, when momentum is larger, all can promotes bubble and move along guide plate.Thereby the angle of inclination of the concrete shape of guide plate and relative level face is not all subject to strict restriction, bubble can be up and not because interrupted causing stops completely, specifically if the mixing of waviness, arc or plane etc. and several shapes is all feasible, adopting surface is that the guide plate of curved surface is as arc or waviness plate, can further increase bubble travel path length, when guide plate is smooth plate, the angle of itself and horizontal plane is preferably 10～30 °, more preferably 20 °.
It may be noted that, when bubble upwards climbs, guide plate relative level face tilt degree is little, and the movement rate of bubble is slow, gas-liquid stirring degree is low, but gas-liquid contact time is long, path length or the gas-to-liquid contact area of bubble operation are larger, and inclined degree is when larger, the movement rate of bubble is very fast, gas-liquid stirring degree is large, and gas-liquid contact time is short, and total path or the gas-to-liquid contact area of bubble operation are less.
The concrete operations of microorganism culturing also can comprise substratum preparation and the pre-treatment such as conventional sterilizing that nutrient solution is carried out, and the operation such as separating-purifying after having cultivated.
The specific embodiment of a microorganism culturing is provided below, and this microbial culture method comprises the following steps:
Treat that culturing micro-organisms is micro-algae Synechococcussp.MA19, the compound method of substratum BG11 is: by SODIUMNITRATE 1.5g, and dipotassium hydrogen phosphate 0.04g, magnesium sulfate (MgSO
47H
2o) 0.075g, calcium chloride (CaCl
22H
2o) 0.036g, citric acid 0.006g, ferric ammonium citrate 0.006g, EDTA0.001g, sodium carbonate 0.02g, trace metal solution 1.0ml, mixed dissolution is in 1L distilled water, and regulating pH is 7.1, puts into refrigerator stand-by after sterilizing.
Micro-algae is introduced to the process of the nutrient solution of incubator and first carried out preculture, preculture comprises the following steps:
S01, in Erlenmeyer flask, micro-10ml algae liquid being mixed with 30ml substratum BG11, at 25 ℃, LED light-illuminating and pass into continuously under the condition of gas and cultivate 24h, obtain micro algae culturing liquid 1, is slightly muddy green liquid;
S02, after 3 days, 35ml micro algae culturing liquid 1 is mixed with 165ml substratum BG11, proceed to cultivate, the same S01 of culture condition, obtains micro algae culturing liquid 2;
S03, after 3 days, the 200ml micro algae culturing liquid 3 that S02 is obtained is divided into three equal parts and is divided in Erlenmeyer flask, adds respectively substratum BG11 to 200ml, continue cultivate, condition is identical with S01, to micro algae culturing liquid become muddiness, obtain micro algae culturing liquid 3;
S04, after 1 day, the micro algae culturing liquid 3 in three Erlenmeyer flasks is merged in an Erlenmeyer flask, add substratum BG11 to 2L, continue to cultivate, the same S01 of culture condition, obtains green muddy micro-algae preculture liquid.
Micro-algae preculture liquid that preculture is obtained adds in the container of high-density microbial cultivation device, then adds 18L substratum BG11, opens the LED lamp in high-density microbial cultivation device, passes into continuously gas simultaneously, cultivates.
Gas in preculture and culturing process is for containing carbonic acid gas 15%(volume fraction), 100ppm nitrogen peroxide, the air of 250ppm sulfurous gas, in culturing process, air input is 1 cubic metre of per minute.
Figure 11 is the cross-sectional view of high-density microbial cultivation device, Figure 12 is the STRUCTURE DECOMPOSITION schematic diagram of high-density microbial cultivation device, Figure 13 is the stereographic map of high-density microbial cultivation device, high-density microbial cultivation device comprise as with the bubbles assembly 1, the bubble generator 6 that are arranged in container, also comprise gas leading-in device.
Container is specially incubator, comprises casing 41, case lid 42 and production well 43, and casing 41 bottom surfaces are about 1 × 1m
2, high about 1m, the nutrient solution liquid level in incubator is higher than bubbles assembly 1, and casing 41 and case lid 42 are made up of plastics or metal or glass or other material respectively, without particular restriction.
Gas leading-in device comprises gas distribution device 51 and intake ducting 52, after the air chamber that gas imports container bottom through intake ducting 52, in nutrient solution, produce through bubble generator 6 bubble that diameter is less, bottom by bubbles assembly 1 enters, with overflow nutrient solution containing the bubble 3 after the nutrient solution generation exchange of substance of micro-algae after discharged by production well 43.Bubble generator 6 is microwell plate, and the packing ring 7 of sealing use is set between air chamber and bubble generator.It can be also the device that microporous pipe or micro-hole aerator or other can form gas micro-bubble in nutrient solution that bubble is sent out generating unit.
The structure of bubbles assembly 1 and assembling mode are referring to Fig. 1～Fig. 5, comprise tortuous ways and mounting block, by the equidistant spread configuration of guide plate along continuous straight runs of same size fixing, obtain assembly parts, assembly parts stack by the assembly parts that overturn after 180 ° with rotation, guide plate contact on tortuous ways is connected, obtains the bubbles assembly of multiple tortuous ways compositions.Concrete, guide plate is long 1 meter, wide 10.6cm, the square transparent plate of thick 1mm, with the angle of horizontal plane be 20 degree, 28 (in figure, only showing a part) vertical direction contact up and down successively connect guide plate form tortuous ways, equal intervals (20mm) is arranged 45 (only showing a part in figure) tortuous ways in the horizontal direction, after fixing, obtain bubbles assembly, bubble alternately upwards climbs along the guide plate lower surface complications of adjacent two tortuous ways, until overflow containing the nutrient solution of micro-algae.
After bubbles assembly 1 is arranged in casing 41, between bubbles assembly 1 and the inwall of casing 41, leave space 8, flow for the nutrient solution containing micro-algae that is subject to bubble agitation, promote the material balance in culture system to distribute, improve culture efficiency, specifically in culturing process, in bubble is advanced along tortuous path upwards in the passage of bubbles assembly 1, also move upward containing the nutrient solution of micro-algae thereupon, then with bubble capable go out the rear space 8 between casing 41 and bubbles assembly 1 of bubble guidance set 1 be back to casing 41 bottoms.
In this incubator, mounted LED lamp on the guide plate of bubbles assembly 1, projects light onto around bubble, micro-algae can be obtained simultaneously and carry out the essential carbonic acid gas of photosynthesis and luminous energy, makes micro-algae can effectively carry out photosynthesis and growth rapidly.
In the bubbles assembly 1 of the present embodiment, bubble is being 2.99m containing the travel path length in the nutrient solution of micro-algae, and relatively vertically travel distance (1m) improves closely 200%, and bubble 3 is 134m with the contact area of the nutrient solution containing micro-algae
2, than its direct area 1m in the time that vertical direction is advanced
2increase by 134 times, significantly improved the gas-to-liquid contact area in unit volume, promote the culturing process of gas-liquid exchange of substance and microorganism.Meanwhile, in the process that bubble is upwards advanced in complications, drive containing the nutrient solution of micro-algae and flow, improve gas-liquid agitation frequency, promote material balance, improve microorganism culturing efficiency.Along with the continuous merisis of micro-algae, the light transmission of nutrient solution sharply reduces, and microorganism is main only effectively to grow in the region that can receive light, and the superiority of this device just fully demonstrates out.
In micro-algae culturing process, by observing the green growing state that changes and detect nutrient solution optical density(OD) and change to weigh micro-algae, test-results shows, adopt this device to carry out micro-algae cultivation, per minute can be processed 297 grams of carbonic acid gas, 0.26 gram of nitrogen peroxide, 0.66 gram of sulfurous gas, and produce 162 grams of micro-algal biomass, culture efficiency significantly improves.
When this high-density microbial cultivation device is applied to off gas treatment, need in nutrient solution, introduce corresponding microorganism, as process carbonic acid gas introduce microorganism using carbonic acid gas as carbon source, process sulfur oxide introduce can metabolism sulfur oxide microorganism, process nitrogen oxide introduce can the microorganism of metabolism nitrogen oxide in, the bubble generator that when processing, waste gas is imported to culture apparatus forms bubble in nutrient solution, for microorganism provides gas nutrition.
Above content is in conjunction with concrete preferred implementation further detailed description of the utility model, can not assert that embodiment of the present utility model is confined to these explanations.For the utility model person of an ordinary skill in the technical field, without departing from the concept of the premise utility, can also make some simple deduction or replace, all should be considered as belonging to protection domain of the present utility model.
Claims (8)
1. a bubbles assembly, is characterized in that: it comprises the tortuous ways that at least two along continuous straight runs are arranged; Described tortuous ways comprises that at least two at vertical direction successively connected guide plate up and down, described guide plate relative level face tilt arranges, and the vergence direction of the guide plate relative level face of arbitrary neighborhood is contrary in same tortuous ways, between the tortuous ways of arbitrary neighborhood, form the tortuous passage rising; Arbitrary guide plate of described tortuous ways and the hithermost guide plate of adjacent tortuous ways are overlapping in the projection section of horizontal plane.
2. bubbles assembly according to claim 1, is characterized in that: it also comprises the mounting block for fixing described tortuous ways.
3. bubbles assembly according to claim 2, is characterized in that: described guide plate is smooth plate or arc or waviness plate.
4. bubbles assembly according to claim 3, is characterized in that: described guide plate is smooth plate, and the angle of itself and horizontal plane is 10～30 °.
5. according to claim 1～4 any one institute bubbles assembly, it is characterized in that: adjacent described tortuous ways be spaced apart 15-50mm.
6. a high-density microbial cultivation device, it is characterized in that: described high-density microbial cultivation device comprises container, bubble generator, at least one bubbles assembly as described in claim 1～5 any one, described bubble generator and bubble guidance set are arranged in described container, and described bubble generator is arranged on the bottom of bubbles assembly.
7. high-density microbial cultivation device according to claim 6, is characterized in that: between described bubbles assembly and the inwall of described container, leave for the mobile gap of nutrient solution.
8. according to the high-density microbial cultivation device described in claim 6 or 7, it is characterized in that: on described tortuous ways, be provided with light source.
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Cited By (1)
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| WO2015042950A1 (en) * | 2013-09-30 | 2015-04-02 | 钟琦 | Bubble-guiding assembly, device for culturing high-density microorganisms and use thereof |
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