CN102260629A - Plate-type photobioreactor - Google Patents
Plate-type photobioreactor Download PDFInfo
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- CN102260629A CN102260629A CN2010101938374A CN201010193837A CN102260629A CN 102260629 A CN102260629 A CN 102260629A CN 2010101938374 A CN2010101938374 A CN 2010101938374A CN 201010193837 A CN201010193837 A CN 201010193837A CN 102260629 A CN102260629 A CN 102260629A
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- bioreactor
- board
- baffle plate
- runner
- lower baffle
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
Abstract
The invention relates to a plate-type photobioreactor, which comprises at least one flow channel, wherein each flow channel comprises at least 2 upper baffle plates, which are arranged on the inner wall face on a light receiving side, and at least 2 lower baffle plates, which are arranged on the inner wall face on a backlight side; and the included angles of the length directions of the upper and lower baffle plates and the flowing direction of culture solution are 20 to 70 DEG and are opposite. The plate-type photobioreactor has a specific internal structure, allow microalgae cells to move back and froth between the bright and dark areas in the photobioreactor, and give play to the 'flash effect' of the microalgae cells, thereby improving the light energy utilization and microalgae cells yield of the microalgae large-scale culture. The plate-type photobioreactor disclosed by the invention is suitable for large-scale culture of various microalgae cells.
Description
Technical field
The present invention relates to a kind of board-like bioreactor, specifically relate to a kind of board-like bioreactor with special internal structure.
Background technology
Microalgae cell is rich in the biological substance of multiple high added values such as amino acid, protein, VITAMIN, unsaturated fatty acids, has become the important materials source of human food prods, medicine, dyestuff, field of fine chemical.At present, exhaustion day by day along with fossil energies such as oil, coals, caused people's great attention based on the biorefinery of biomass, little algae can provide a large amount of biomass (grease, starch, Mierocrystalline cellulose) as important renewable energy, in the biorefinery field, have broad application prospects.
Micro-algae large-scale is cultivated and mainly is divided into open cultivation and closed cultivation dual mode.Open cultivation, be to develop the earliest and use the most general a kind of mode, countries in the world, particularly China are still with its main mode (Borowitzka L.T., Bioresource Technology that cultivates as little algae industrialization at present, 1991,38:251～252).In the open cultivation, representational reactor comprises racetrack cultivation pool, circular cultivation pool.The major advantage of this training method is that structure is simple, with low cost and easy and simple to handle.Shortcoming is that the efficiency of light energy utilization is low, is subjected to the influence of external environment factor big, and easily contaminated, moisture evaporation is big.
The closed photo bioreactor of various ways (as pillar, tubular type and board-like) is adopted in closed cultivation usually.Than open cultivation, closed cultivation is not easy contaminated, the conserve water resource, and the culture density height, the results cost is low, and shortcoming is cost of investment height (Pulz O., Applied Microbiology andBiotechnology, 2001,57:287～293; Lee Y-K., Journal of Applied Phycology, 2001,13:307～315; Tsygankov A.A., Applied Biochemistry and Microbiology, 2001,37 (4): 333～341).In order to overcome closed cultivation defect of high cost, need on the one hand to reduce to cultivate required material, energy consumption cost, need to increase substantially productive rate on the other hand.Though the productive rate of closed cultivation will be higher than open cultivation, the efficiency of light energy utilization of little algae is also very low.Managing to improve frustule is the important channel of improving productive rate, reducing cost to the utilization ratio of luminous energy.
Because the photosynthesis process of little algae can be divided into two stages, is called photoresponse and dark reaction.At photoreaction stage, frustule is accepted photon and is converted to chemical energy; In cloudy, turbid phase, frustule utilizes chemical energy synthetic cell component, and in cloudy, turbid phase, frustule does not need illumination even illumination harmful on the contrary.Therefore, for single algae cell, the illumination that continues means the waste of photon.
Scientists has been understood, in the bioreactor that scale is cultivated and under the common cell density, and decay rapidly when light is propagated in nutrient solution, the penetration range of light is several millimeters, has only about 1mm under high-cell density.In fact can be divided in the bioreactor near the light district of plane of illumination inner-wall surface with outside dark space two portions.If frustule with CF (usually above the frequency of 1Hz) when the light district of bioreactor and the dark space frequent replacement, can produce " sparkle effect ", the utilization ratio of luminous energy (the Janssen M that can be greatly improved, Slenders P, Tramper J, et al., Enzyme MicrobialTechnology, 2001,29:298～305; Matthijs H.C.P, Balke H, Mur L.R, et al., Biotechnology and Bioengineering, 1996,50:98～107).Promptly, when frustule shuttles between the light district of bioreactor and dark space, just can make the frustule of accepting illumination in time enter the dark space and carry out dark reaction, make the frustule of having finished dark reaction get back to the light district simultaneously and accept illumination once more, so just make the photon that enters bioreactor be fully utilized.Therefore by the mixing of hot housing liquid, frustule high frequency between light district and dark space is shuttled, can improve the overall efficiency of light energy utilization and the output of frustule.
Yet in traditional board-like bioreactor, the mobile mixing of nutrient solution is still undesirable.The thickness of slab of board-like bioreactor (comprising horizontal positioned, inclination placement, vertical placement etc.) does not wait between 1～10cm, and the inner-wall surface of these bioreactors is slick often.Be subjected to the restriction of reactor wall, in close inner-wall surface place nutrient solution undercompounding, frustule is very little perpendicular to the speed of flow direction (to call the optical path direction in the following text), be difficult to be created in shuttling back and forth between light district and the dark space, frustule can produce light inhibition or even light injury (being that illumination is excessive) near being positioned at the plane of illumination inner-wall surface for a long time; And be in light restriction state (being the illumination deficiency) for a long time apart from plane of illumination inner-wall surface frustule far away, make the efficiency of light energy utilization not high.
Design by certain internal structure can hot housing liquid mixing.As the disclosed box bioreactor of the plate of putting hollow weir that adds in Chinese patent CN200610026539.X, by this specific interior arrangement can hot housing liquid mobile mixing, simultaneously, inner light source or as distribution device in gas-fluid can be installed in described hollow weir.Though such device that installs hollow weir additional can play the mixing effect of hot housing liquid, complex structure, the manufacturing cost height, and also the mixing of frustule is unordered mixing, is difficult to guarantee that frustule is subjected to the illumination of specific period.
Summary of the invention
The objective of the invention is on the basis of existing board-like bioreactor, provide a kind of simple in structure, be convenient to the board-like bioreactor made in batches, it has specific internal, can realize frustule shuttling between the light district of bioreactor and dark space, can bring into play " sparkle effect " of frustule, thereby improve the microalgae mass efficiency of light energy utilization of cultivating and the output that improves microalgae cell.Board-like bioreactor of the present invention is suitable for mass-producing and cultivates various little algaes.
Board-like bioreactor provided by the invention comprises at least one runner, and wherein, each runner comprises:
-at least 2 overhead gages are arranged on the inner-wall surface of plane of illumination;
-at least 2 lower baffle plates are arranged on the inner-wall surface of matte surface;
Wherein, the corner dimension of the length direction of overhead gage and lower baffle plate and nutrient solution flow direction is 20～70 degree, and the angle direction is opposite.
In embodiments of the present invention, described board-like bioreactor is hardware bioreactor or software bioreactor.Described hardware bioreactor comprises the plane of illumination that made by hard plate material and matte surface and separate along the bearing rib of nutrient solution flow direction setting that thereby to form the cross section be the orthogonal runner.Described software bioreactor is directly plane of illumination and matte surface to be bonded together in the position that soft material (as coiled material or film material) need be provided with gusset with methods such as hot pressing or bondings, strutted and form the cross section and be circular or oval-shaped runner thereby charge into behind the nutrient solution runner, the effect of gusset is still played at bonded position.
In embodiments of the present invention, on the longshore current road length direction, the spacing of described overhead gage or the spacing of lower baffle plate are 10mm～150mm.
In embodiments of the present invention, the projecting length of described upper and lower baffle plate longshore current road width is 30～100% of this board-like bioreactor width of flow path.
In embodiments of the present invention, the height of described upper and lower baffle plate (height in the cross section of baffle plate own) is 1mm～10mm.
In embodiments of the present invention, the cross section of described upper and lower baffle plate can be rectangle, trapezoidal, trilateral, semicircle or other shape; Wherein, the thickness of the baffle plate of rectangular shape is 1mm～5mm; The thickness of half eminence of trapezoidal, trilateral or semicircular baffle plate is 1mm～5mm.
In embodiments of the present invention, the material of described baffle plate can be light transmissive material or select the light-proof material of processing installation easily.Described light transmissive material can be a kind of in glass, synthetic glass, the plastics (polyethylene, polyvinyl chloride, polypropylene, polyester etc.) etc.; Described light-proof material can be a kind of in plastics, rubber, the resin etc.Preferably, the material of described baffle plate is identical with the material of plane of illumination.
Described plane of illumination is to be made by various light transmissive materials, as glass, synthetic glass, plastics (polyethylene, polyvinyl chloride, polypropylene, polyester etc.).
In embodiments of the present invention, the length of described runner can be cultivated needs according to reality and be done corresponding the adjustment, and a plurality of runners parallel connections or series connection can be used, and forms the board-like bioreactor of different profiles.This moment, gusset played the effect of traverse baffle simultaneously.
During scale operation, can or connect supporting again other device with a plurality of board-like bioreactors parallel connections of the present invention according to the requirement of output.
The board-like bioreactor of the efficiency of light energy utilization that raising microalgae mass of the present invention is cultivated, can be built in the outdoor open-air or booth, make full use of natural light, can cultivate, comprise spirulina, chlorella, salt algae, diatom, chrysophyceae etc. various little algaes.
Compared with prior art, the advantage of the board-like bioreactor of the efficiency of light energy utilization that raising microalgae mass of the present invention is cultivated is: " sparkle effect " that can give full play to frustule, improve the efficiency of light energy utilization that microalgae mass is cultivated, improve the output of unit irradiation area.In addition, the board-like bioreactor of the efficiency of light energy utilization that raising microalgae mass of the present invention is cultivated is simple in structure, is convenient to make in batches, and the number that can increase reactor simply expands the scale of production.
Description of drawings
The skeleton view of one runner of Fig. 1, the board-like bioreactor of hardware of the present invention;
The right view of one runner of Fig. 2, the board-like bioreactor of hardware of the present invention;
The vertical view of the board-like bioreactor of Fig. 3, a plurality of runner parallel connections of the present invention;
The vertical view of Fig. 4, the placed in-line board-like bioreactor of a plurality of runners of the present invention;
The front view section of the baffle plate of Fig. 5, several different cross section shapes in the present invention's one runner;
The change in location of frustule on the optical path direction in Fig. 6, the board-like bioreactor of the present invention;
One cross section of Fig. 7, the board-like bioreactor of software of the present invention is the right view of the runner of circle;
One cross section of Fig. 8, the board-like bioreactor of software of the present invention is the right view of oval-shaped runner;
Fig. 9, microalgae culture system synoptic diagram;
Wherein:
1. the runner nutrient solution 2. runner nutrient solutions that enter the mouth export 3. plane of illuminations
4-1. overhead gage 4-2. lower baffle plate 5. matte surfaces
6. 7. rectangle baffle plate 8. trapezoidal baffle plates of gusset (traverse baffle)
9. trilateral baffle plate 10. fluid delivery systems 11. bioreactors
12. heat exchanger 13. gas-liquid switches 14 end plates
The 16. reactor nutrient solutions outlet 15. the reactor nutrient solution enters the mouth
α. the angle of nutrient solution flow direction in the length direction of overhead gage and the runner
β. the angle of nutrient solution flow direction in the length direction of lower baffle plate and the runner.
Embodiment
Board-like bioreactor provided by the invention comprises at least one runner, and wherein, each runner comprises :-at least 2 overhead gage 4-1 are arranged on the inner-wall surface of plane of illumination 3;-at least 2 lower baffle plate 4-2 are arranged on the inner-wall surface of matte surface 5; Wherein, the corner dimension of the length direction of overhead gage and lower baffle plate and nutrient solution flow direction is 20～70 degree, and the angle direction is opposite.
One embodiment of the present invention, described board-like bioreactor is the hardware bioreactor, as depicted in figs. 1 and 2, it comprise plane of illumination (top panel) 3 that make by hard plate material and matte surface (lower panel) 5 and along nutrient solution flow direction (promptly by the direction of runner nutrient solution inlet 1) to runner nutrient solution outlet 2 bearing rib that is provided with 6 separates that thus to form the cross section be the orthogonal runner.Wherein, each runner comprises: at least 2 overhead gage 4-1 are arranged on the inner-wall surface of plane of illumination 3; At least 2 lower baffle plate 4-2 are arranged on the inner-wall surface of matte surface 5; The size of the angle β of the size of the angle α of the length direction of overhead gage and nutrient solution flow direction and the length direction of lower baffle plate and nutrient solution flow direction is 20～70 degree, and the direction of angle α and angle β opposite (as shown in Figure 3, Figure 4).
Another embodiment of the present invention, described board-like bioreactor is the software bioreactor, as shown in Figure 7 and Figure 8, it directly bonds together plane of illumination 3 and matte surface 5 in the position that soft material (as coiled material or film material) need be provided with gusset with methods such as hot pressing or bondings, strutted and form the cross section and be circular or oval-shaped runner thereby charge into behind the nutrient solution runner, the effect of gusset (traverse baffle) is still played at bonded position.Wherein, each runner comprises: at least 2 overhead gage 4-1 are arranged on the inner-wall surface of plane of illumination 3; At least 2 lower baffle plate 4-2 are arranged on the inner-wall surface of matte surface 5; The size of the angle β of the size of the angle α of the length direction of overhead gage and nutrient solution flow direction and the length direction of lower baffle plate and nutrient solution flow direction is 20～70 degree, and the direction of angle α and angle β opposite (as shown in Figure 3, Figure 4).
In embodiments of the present invention, on the longshore current road length direction, the spacing of described overhead gage or the spacing of lower baffle plate are 10mm～150mm.
In embodiments of the present invention, shown in Fig. 2,3,4,7,8, the projecting length of described upper and lower baffle plate longshore current road width is 30～100% of this board-like bioreactor width of flow path.
In embodiments of the present invention, the height of described upper and lower baffle plate (height in the cross section of baffle plate own) is 1mm～10mm.
In embodiments of the present invention, as shown in Figure 5, the cross section of described upper and lower baffle plate can be rectangle, trapezoidal, trilateral, semicircle or other shape; Wherein, the thickness of the baffle plate of rectangular shape is 1mm～5mm; The thickness of half eminence of trapezoidal, trilateral or semicircular baffle plate is 1mm～5mm.
In embodiments of the present invention, the material of described baffle plate can be light transmissive material or select the light-proof material of processing installation easily.Described light transmissive material can be a kind of in glass, synthetic glass, the plastics (polyethylene, polyvinyl chloride, polypropylene, polyester etc.) etc.; Described light-proof material can be a kind of in plastics, rubber, the resin etc.Preferably, the material of described baffle plate is identical with the material of plane of illumination.
Described plane of illumination is to be made by various light transmissive materials, as glass, synthetic glass, plastics (polyethylene, polyvinyl chloride, polypropylene, polyester etc.).
In embodiments of the present invention, the plane of illumination of each runner in the described hardware bioreactor and the spacing between matte surface are 10mm～100mm, and the width of runner is 10mm～300mm.
In embodiments of the present invention, the plane of illumination and the matte surface both sides of each runner in the described software bioreactor are bonding, and distance is 10mm～300mm between two bonding parts.
In one embodiment of the present invention, the length of described runner can be cultivated needs according to reality and be done corresponding the adjustment, can make a plurality of runner parallel connections with the double traverse baffle of doing of gusset, as shown in Figure 3; Or make a plurality of runner series connection, as shown in Figure 4.
Can select the manufacturing and the mounting means of baffle plate according to Drawing abillity.For example, can with make on the inner-wall surface that good baffle plate be bonded in board-like bioreactor separately, baffle plate with mould that the plane of illumination 3 of board-like bioreactor is one-body molded with baffle plate with matte surface 5 or that will make in advance is connected postposition with support and goes in the board-like bioreactor to make baffle plate be close to the plane of illumination 3 of board-like bioreactor and the inner-wall surface of matte surface 5.
During scale operation, can or connect supporting again other device with a plurality of board-like bioreactors parallel connections of the present invention according to the requirement of output.For example, as shown in Figure 9, cultivate in the process of frustule in actual production, the board-like bioreactor 11 of the efficiency of light energy utilization of cultivating except above-mentioned raising microalgae mass of the present invention in the whole culture systems, also being furnished with gas-liquid switch 13 is used for air feed and carries out oxygen parsing, fluid delivery system 10, heat exchanger 12, coupling device, material distribution device, temperature monitoring control device, and other auxiliary equipment.
The principle of board-like bioreactor of the present invention is: when nutrient solution is crossed board-like bioreactor of the present invention with certain velocity flow, can in runner, form spiral motion, and can between baffle plate, produce whirlpool, promote frustule and on the optical path direction, form certain to-and-fro movement, thereby realize that frustule is in the light district of board-like bioreactor and shuttling of dark space.By the flow velocity of adjusting nutrient solution, the size of baffle plate, the distance of baffle plate, can obtain the dark loop cycle of different light, and the dark round-robin frequency of light can be higher than 1Hz.What Fig. 6 represented is in the board-like photoreactor of a hardware, the spacing that the length direction of the plane of illumination 3 of runner and matte surface 5 internal surface spacing 16mm, overhead gage and lower baffle plate becomes miter angle with the nutrient solution flow direction and is full of width of flow path, baffle plate be 80mm, height of baffle plate be 4mm, when baffle plate thickness is 2mm, when representing the particle of frustule to move in reactor, its position on the optical path direction over time.Can be clear that particle is fluctuation regularly on the optical path direction, the dark loop cycle of complete light is about 160ms.When the nutrient solution flow velocity in the board-like bioreactor is 10cm/s～100cm/s, in this scope, can form the dark loop cycle of light of 500ms～20ms, the corresponding dark cycle frequency of light is 2Hz～50Hz.
The board-like bioreactor of the efficiency of light energy utilization that raising microalgae mass of the present invention is cultivated, can be built in the outdoor open-air or booth, make full use of natural light, can cultivate, comprise spirulina, chlorella, salt algae, diatom, chrysophyceae etc. various little algaes.
Under shining, outdoor natural light carries out the cultivation of spirulina.The board-like bioreactor horizontal positioned of the culture systems that makes up, the manufacturing material is a poly (methyl methacrylate) plate, and the thickness of upper and lower panel is 2mm, and the thickness of end plates 14 and gusset 6 is 2mm, and the length of gusset 6 is 80cm.Board-like bioreactor is of a size of: long 1m, wide 1.022m, upper and lower panel internal surface spacing 16mm, dull and stereotyped inside is divided into 10 by gusset and respectively is the wide runner of 10cm, each runner parallel connection (as Fig. 3).Be close to the inboard square-section baffle plate of pasting by the preparation of poly (methyl methacrylate) plate material 3 of top panel and lower panel, the corner dimension of the length direction of overhead gage and lower baffle plate and nutrient solution flow direction is 45 degree, and the angle direction is opposite, baffle length 14.1cm (baffle plate along the projecting length of nutrient solution width of flow path direction be width of flow path 100%), the spacing of longshore current road length direction overhead gage is 80mm, height of baffle plate is 4mm, and baffle plate thickness is 2mm.Install in each runner 10 overhead gages and 10 lower baffle plates (zone between the baffle plate cover bioreactor effective plane of illumination about 82%).
Culture systems (is divided into two groups by 10 placed in-line above-mentioned board-like bioreactors 11,5 of every group of series connection), fluid delivery system 10 (commercially available surge pump), heat exchanger 12, gas-liquid switch 13 constitutes, mode of connection as shown in Figure 9, wherein board-like bioreactor 11 horizontal positioned.The flow velocity of nutrient solution in reactor is 20cm/s.
The algae kind is from Chinese Academy Of Sciences Process Engineering Research Institute, and kind is a spirulina plalensis, and substratum is the Zarrouk substratum, and the starting point concentration of sodium bicarbonate is 0.1mol/L in the nutrient solution.
Use the water behind 0.2 μ micro-filtrate membrane filtration to clean above-mentioned bioreactor before the cultivation.According to Zarrouk culture medium prescription preparation 160L substratum, substratum is with 0.2 μ micro-filtrate membrane filtration.Liquid, inoculum density 0.1g/L are planted in preparation according to a conventional method.Medium pH value is set in 9.0, and 25～35 ℃ of envrionment temperatures adopt outdoor natural lighting, and the control culture temperature is 32 ± 1 ℃.The period sampling measuring algae cell density.Begin semicontinuous gathering when algae cell density reaches 3g (dry weight)/L, collecting method is that (approximately per 3 days) draw 20% of nutrient solution in the culture systems, and the filtered liquid after filtering is returned in the former culture systems.Frustule results after scouring, drying that filtration obtains.
Regularly detect the concentration of other nutritive salt and in time additional between incubation period, and additional less water is to remedy the evaporation of water loss.Between incubation period, the mixed gas of bubbling air and carbonic acid gas in each the gas-liquid switch in culture systems, the flow of mixed gas is 0.6L/L.min, the molar fraction of carbonic acid gas is 3% in the mixed gas.Culturing process continues 30 days, and the area productive rate of spirulina is about 36g/m
2.d.
Comparative example 1
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, and different is not have baffle plate in the board-like bioreactor.
Culturing process continues 30 days, and the area productive rate of spirulina is 25g/m
2.d.
Obviously, under the same culture condition, area productive rate in the board-like bioreactor of the baffle plate of the embodiment of the invention 1 is higher than the area productive rate in the board-like optical-biological reaction of no baffle plate of comparative example 1, and the board-like bioreactor of baffle plate promptly of the present invention makes the efficiency of light energy utilization of frustule improve obviously.
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, the height of different the is baffle plate of putting into is 2mm, thickness is 1mm, the spacing of longshore current road length direction overhead gage is 30mm, the corner dimension of the length direction of baffle plate and nutrient solution flow direction is 70 degree, baffle plate is 50% of a width of flow path along the projecting length of nutrient solution width of flow path direction, and 25 overhead gages and 25 lower baffle plates are installed in each runner, the zone between the baffle plate cover bioreactor effective plane of illumination about 74%).
Culturing process continues 30 days, and the area productive rate of spirulina is about 34g/m
2.d.Be higher than comparative example 1 area productive rate (25g/mw under the same culture condition in the board-like bioreactor of no baffle plate
2.q), the efficiency of light energy utilization that shows frustule improves obviously.
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, and the height of different the is baffle plate of putting into is 6mm, and thickness is 3mm, and the spacing of longshore current road length direction overhead gage is 100mm, and the length direction of baffle plate becomes 20 degree angles with the nutrient solution flow direction.6 overhead gages and 6 lower baffle plates are installed in each runner, the zone between the baffle plate cover bioreactor effective plane of illumination about 78%).
Culturing process continues 30 days, and the area productive rate of spirulina is about 33g/m
2.d.Be higher than comparative example 1 area productive rate (25g/m under the same culture condition in the board-like optical-biological reaction of no baffle plate
2.d), the efficiency of light energy utilization that shows frustule improves obviously.
Embodiment 4
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, the spacing of different is longshore current road length direction overhead gage is 15mm, baffle plate is 30% of a width of flow path along the projecting length of nutrient solution width of flow path direction, 50 overhead gages and 50 lower baffle plates are installed in each runner, the zone between the baffle plate cover bioreactor effective plane of illumination about 77%)
Culturing process continues 30 days, and the area productive rate of spirulina is about 34g/m
2.d.Be higher than comparative example 1 area productive rate (25g/m under the same culture condition in the board-like optical-biological reaction of no baffle plate
2.d), the efficiency of light energy utilization that shows frustule improves obviously.
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, and different is that nutrient solution inlet flow velocity is 50cm/s.
Culturing process continues 30 days, and the area productive rate of spirulina is about 39g/m
2.d.Be higher than comparative example 1 area productive rate (25g/m under the same culture condition in the board-like optical-biological reaction of no baffle plate
2.d), the efficiency of light energy utilization that shows frustule improves obviously.
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, and different is that nutrient solution inlet flow velocity is 10cm/s.
Culturing process continues 30 days, and the area productive rate of spirulina is about 32g/m
2.d.Be higher than comparative example 1 area productive rate (25g/m under the same culture condition in the board-like optical-biological reaction of no baffle plate
2.d), the efficiency of light energy utilization that shows frustule improves obviously.
Embodiment 7
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, different is, the upper and lower panel internal surface spacing 30mm of board-like bioreactor, the height of the baffle plate of putting into is 10mm, thickness is 5mm, and the spacing of longshore current road length direction overhead gage is 120mm, and the length direction of overhead gage becomes 60 degree angles with the nutrient solution flow direction, the length direction of lower baffle plate becomes 30 degree angles with the nutrient solution flow direction, overhead gage is opposite with the vergence direction that the length direction of lower baffle plate is compared with the flow channel length direction.7 overhead gages and 7 lower baffle plates are installed in each runner, the zone between the baffle plate cover bioreactor effective plane of illumination about 78%).
Culturing process continues 30 days, and the area productive rate of spirulina is about 33g/m
2.d.Be higher than comparative example 2 area productive rate (23g/m under the same culture condition in the board-like optical-biological reaction of no baffle plate
2.d), the efficiency of light energy utilization that shows frustule improves obviously.
Comparative example 2
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 7, and different is not have baffle plate in the board-like bioreactor.
Culturing process continues 30 days, and the area productive rate of spirulina is 23g/m
2.d.
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 1, and different is, the upper and lower panel internal surface spacing 30mm of board-like bioreactor, and the cross-sectional shape of the baffle plate of putting into is a trilateral, and the height of baffle plate is 8mm, and the thickness of half eminence is 3mm.
Culturing process continues 30 days, and the area productive rate of spirulina is about 32g/m
2.d.Be higher than comparative example 2 area productive rate (23g/m under the same culture condition in the board-like optical-biological reaction of no baffle plate
2.d), the efficiency of light energy utilization that shows frustule improves obviously.
Embodiment 9
Under shining, outdoor natural light carries out the cultivation of spirulina.Other condition is with embodiment 1, and different is that board-like bioreactor (as Fig. 3) is the board-like bioreactor of being made by polyethylene film of software, and polyethylene film thickness is 0.2mm.Plane of illumination and matte surface are bonded together in the position that gusset is set as shown in Figure 3 with hot pressed method, the length of plate-type reactor is 1m, and width is 1.022m, by bonding board-like bioreactor is divided into 10 runners that 10cm is wide.By plane of illumination and matte surface inboard the form inwardly protruding (baffle plate 4) of built-in externally hot pressed mode of mould while at each runner, the cross-sectional shape of projection is trilateral (as 9 among Fig. 5), the height 4mm of projection, halfwidth degree 2mm, the length direction of projection (baffle plate) and the corner dimension of nutrient solution flow direction are 45 degree, and the length direction of the projection of plane of illumination and matte surface is opposite with the angle direction of nutrient solution flow direction, projection is 10cm along the projecting length of nutrient solution width of flow path direction, and the spacing of projection (baffle plate) is 80mm on the longshore current road length direction.Plane of illumination and matte surface inboard respectively are provided with 10 projectioies (baffle plate) in each runner, about 82% of effective plane of illumination of the zone covering bioreactor between the projection.
During actual the cultivation, when nutrient solution was pumped into board-like bioreactor, each runner is strutted to long diameter (wide) to be about 8cm, to lack the elliptical shape (as Fig. 8) that diameter (height) is about 3.6cm.
The culture volume of preparation is about 170L, and control condition and collecting method are with embodiment 1, and the density when gathering is about 5g/L.
Regularly detect the concentration of other nutritive salt and in time additional between incubation period, and additional less water is to remedy the evaporation of water loss.Between incubation period, the mixed gas of bubbling air and carbonic acid gas in each the gas-liquid switch in culture systems, the flow of mixed gas is 1.0L/L.min, the molar fraction of carbonic acid gas is 3% in the mixed gas.Culturing process continues 30 days, and the area productive rate of spirulina is about 38g/m
2.d.
Comparative example 3
Under natural lighting, carry out the cultivation of spirulina.Other condition is with embodiment 9, and different is not have projection (baffle plate) in the board-like bioreactor.
Culturing process continues 30 days, and the area productive rate of spirulina is about 26g/m
2.d.
Obviously, under the same culture condition, area productive rate in the board-like bioreactor of the baffle plate of the embodiment of the invention 9 is higher than the area productive rate in the board-like optical-biological reaction of no baffle plate of comparative example 3, and the board-like bioreactor of baffle plate promptly of the present invention makes the efficiency of light energy utilization of frustule improve obviously.
Claims (10)
1. a board-like bioreactor comprises at least one runner, it is characterized in that, each runner comprises:
-at least 2 overhead gages are arranged on the inner-wall surface of plane of illumination;
-at least 2 lower baffle plates are arranged on the inner-wall surface of matte surface;
Wherein, the corner dimension of the length direction of overhead gage and lower baffle plate and nutrient solution flow direction is 20～70 degree, and the angle direction is opposite.
2. board-like bioreactor according to claim 1, wherein, described board-like bioreactor is hardware bioreactor or software bioreactor.
3. board-like bioreactor according to claim 2, wherein, thus described hardware bioreactor comprises the plane of illumination that made by hard plate material and matte surface and separate along the bearing rib of nutrient solution flow direction setting that to form the cross section be the orthogonal runner.
4. board-like bioreactor according to claim 2, wherein, described software bioreactor is directly in the position that soft material need be provided with gusset plane of illumination and matte surface to be bonded together, and is strutted and forms the cross section and be circular or oval-shaped runner thereby charge into behind the nutrient solution runner.
5. board-like bioreactor according to claim 1, wherein, on the longshore current road length direction, the spacing of described overhead gage or the spacing of lower baffle plate are 10mm～150mm.
6. board-like according to claim 1 or 5 bioreactor, wherein, the projecting length of described upper and lower baffle plate longshore current road width is 30～100% of this board-like bioreactor width of flow path.
7. board-like according to claim 1 or 5 bioreactor, wherein, the height of described upper and lower baffle plate is 1mm～10mm.
8. board-like according to claim 1 or 5 bioreactor, wherein, the cross section of described upper and lower baffle plate is rectangle, trapezoidal, trilateral, semicircle; The thickness of the baffle plate of rectangular shape is 1mm～5mm; The thickness of half eminence of trapezoidal, trilateral or semicircular baffle plate is 1mm～5mm.
9. board-like according to claim 1 or 5 bioreactor, wherein, the material of described baffle plate is transparent glass, synthetic glass, plastics, or lighttight plastics, rubber, resin.
10. board-like according to claim 1 or 5 bioreactor, wherein, the in parallel or series connection use of described runner.
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| WO2013149442A1 (en) * | 2012-04-05 | 2013-10-10 | 华东理工大学 | Novel internal component and photobioreactor based on enhanced mixing along light direction |
| CN103421677A (en) * | 2013-04-26 | 2013-12-04 | 中国科学院过程工程研究所 | Baffle plate public pool used for large-scale cultivation of microalgae |
| CN103911274A (en) * | 2012-11-30 | 2014-07-09 | 新奥科技发展有限公司 | Device and method for large-scale culture of microalgae |
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| CN104293673A (en) * | 2013-07-19 | 2015-01-21 | 中国石油化工股份有限公司 | Method for large-scale cultivation of microalgae |
| CN104293674A (en) * | 2013-07-19 | 2015-01-21 | 中国石油化工股份有限公司 | Method for large-scale cultivation of microalgae |
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| CN104789442A (en) * | 2014-01-17 | 2015-07-22 | 中国石油化工股份有限公司 | Method for culturing photosynthetic organisms |
| CN105002086A (en) * | 2015-07-16 | 2015-10-28 | 中国海洋大学 | Runway pool microalgae culture system using micro-bubble continuous gas flotation to harvest algal cells |
| CN105018331A (en) * | 2015-07-16 | 2015-11-04 | 中国海洋大学 | Multidirectional-turbulence and efficient-mixing novel runway pond microalgae cultivation system |
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| WO2013149442A1 (en) * | 2012-04-05 | 2013-10-10 | 华东理工大学 | Novel internal component and photobioreactor based on enhanced mixing along light direction |
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