CN104293674A - Method for large-scale cultivation of microalgae - Google Patents
Method for large-scale cultivation of microalgae Download PDFInfo
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- CN104293674A CN104293674A CN201310306168.0A CN201310306168A CN104293674A CN 104293674 A CN104293674 A CN 104293674A CN 201310306168 A CN201310306168 A CN 201310306168A CN 104293674 A CN104293674 A CN 104293674A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
Abstract
The invention discloses a method for large-scale cultivation of microalgae. The method includes cultivating microalgae in a photobioreactor under microalgae cultivation conditions. The reactor comprises a runner (1) and spoilers (2). The spoilers (2) are formed by extension of turbulence sections (23) along a first direction (Y). Each turbulence section (23) comprises a first side (231), a second side (232) and a third side (233) that are connected in order into a closed shape, and the three sides extend along the first direction (Y) respectively to form a first turbulence surface (21), a second turbulence surface (22) and a base surface. The first side (231) and the second side (232) are concave curved sides, the base surface is attached to the inner surface of the runner (1), the first turbulence surface (21) and the second turbulence surface (22) are arranged obliquely relative to the base surface, and the first direction (Y) crosses with the extension direction (X) of the runner (1). The method provided by the invention can improve the yield of microalgae.
Description
Technical field
The present invention relates to the method for the micro-algae of a kind of pilot scale culture.
Background technology
In recent years, one of micro-algae important source material being considered to bioenergy, is the long term policy solving future source of energy crisis, becomes the focus of research.At present, the pilot scale culture of micro-algae is the bottleneck of the micro-algae Biological Energy Industry chain development of restriction, how to realize micro-algae large-scale, the cultivation of high yield is one of problem demanding prompt solution.
Pass the principal element only affecting micro-algae large-scale and cultivate, directly affect the photosynthetic efficiency of micro-algae, growth conditions and the efficiency of light energy utilization.In the R&D process of microdisk electrode, the photosynthetic feature of micro-algae can be utilized to improve photosynthetic efficiency, e.g., when the displacement of frustule between light district and dark space reaches certain frequency (usually above 1Hz), " sparkle effect " (Janssen M will be there is, Slenders P, Tramper J, Mur L R, Wijffels R.Enzyme Microbial Technology, 2001,29:298-305), the photosynthetic efficiency of micro-algae can be improved.
Microdisk electrode generally carries out in bioreactor, and bioreactor can be divided into Race-way photobioreactor and closed photo bioreactor.
Race-way photobioreactor take raceway pond as representative, and advantage is that construction cost is low, energy consumption is low, easy care; Shortcoming be mainly micro-concentration of algae lower, affect greatly by outside environmental elements, floor space is large, water evaporation quantity is large.Raceway pond bioreactor, algae liquid thickness is generally 15-30cm in actual applications, often promotes algae liquid in pond internal recycle, mixing in the mode of wheel rotation, prevents frustule precipitation adsorption and increases the contact probability of micro-algae and light.But in actual applications, the deficiency of raceway pond displays gradually, and in primary stage of inoculation, micro-concentration of algae is lower, light can arrive darker position, micro-algae can obtain sufficient illumination and carry out photosynthesis, and along with the growth of micro-algae, concentration is increasing, optical attenuation is serious, although the frustule of lower floor can be made to move to upper strata by stirring, far can not meet the needs of micro-algae to light, being difficult in unit volume obtain higher biomass.
Closed photo bioreactor, such as tubular type, board-like, pillar etc., advantage be can improve the concentration of microdisk electrode, simplify post-production, the various somatomedin of micro-algae can control, not vulnerable to pollution, good product quality; Deficiency is that the cost of incubator own is high, energy consumption is high in culturing process.Part investigator thinks that closed photo bioreactor is the developing direction that micro-algae large-scale is cultivated, and has carried out large quantifier elimination, have developed multiple closed photo bioreactor, has promoted the development of micro-algae bioenergy.Tubular type, pillar and board-like bioreactor are typical closed photo bioreactors, have also developed a lot of Novel photo-biological reactor on this basis, although to a certain degree overcoming the deficiency of Race-way photobioreactor, the photosynthetic efficiency of micro-algae and the efficiency of light energy utilization are still lower.
Therefore, no matter for Race-way photobioreactor or closed photo bioreactor, the output of micro-algae in micro-algae large-scale culturing process still need be improved at present further in the mode of low cost.
Summary of the invention
The object of the invention is the output in order to improve the micro-algae of open and enclosed bioreactor pilot scale culture, providing the method for the micro-algae of a kind of pilot scale culture.
To achieve these goals, the invention provides the method for the micro-algae of a kind of pilot scale culture, under described method is included in the condition of cultivating micro-algae, the cultivation of micro-algae is carried out in bioreactor, wherein, described reactor comprises runner and the flow-disturbing part of the liquid-flow allowed containing micro-algae, described flow-disturbing part is extended along first direction by flow-disturbing cross section and is formed, described flow-disturbing cross section comprises and connects into the first close-shaped limit in turn, Second Edge and the 3rd limit, described first limit, described in described Second Edge and described 3rd edge, first direction extends and forms the first flow-disturbing face respectively, second flow-disturbing face and basal plane, described first limit and described Second Edge are recessed bent limit, the internal surface that described basal plane fits in described runner is arranged, described first flow-disturbing face and described second flow-disturbing face are arranged relative to described basal planes tilted, described first direction intersects with the bearing of trend of described runner.
Preferably, described first flow-disturbing face and described second flow-disturbing are facing to described basal planes tilted.
Preferably, described first flow-disturbing face is than the entrance of described second flow-disturbing face closer to described runner, the angle of the straight flange that the two-end-point on the straight flange that the two-end-point on described first limit is formed and described 3rd limit is formed is the first angle, the angle of the straight flange that the two-end-point on the straight flange that the two-end-point of described Second Edge is formed and described 3rd limit is formed is the second angle, and described first angle is less than or equal to described second angle.
Preferably, described first angle is 30-60 degree, and described second angle is 45-80 degree.
Preferably, the flow velocity of the described liquid-flow containing micro-algae is 0.3-2m/s.
Preferably, described first limit has first curvature radius, and described Second Edge has second curvature radius, and described first curvature radius is more than or equal to described second curvature radius; More preferably, described first curvature radius is 2-10 times of the height of flow-disturbing part, and described second curvature radius is 1-5 times of the height of flow-disturbing part.
The method of the micro-algae of pilot scale culture of the present invention, can increase the motion vertically of micro-algae, makes to shuttle back and forth between Wei Zaoguang district and dark space, thus improves the efficiency of light energy utilization and the photosynthetic efficiency of micro-algae, improve the output of micro-algae.
Other features and advantages of the present invention are described in detail in embodiment part subsequently.
Accompanying drawing explanation
Fig. 1 is the front elevational schematic of the bioreactor according to one embodiment of the present invention.
Fig. 2 is the schematic top plan view of bioreactor as shown in Figure 1.
Fig. 3 is the schematic side view of five kinds of embodiments of bioreactor of the present invention.
Fig. 4 is the schematic diagram in the flow-disturbing cross section of the flow-disturbing part of bioreactor of the present invention.
Description of reference numerals
1 runner; 2 flow-disturbing parts; 21 first flow-disturbing faces; 22 second flow-disturbing faces; 23 flow-disturbing cross sections; 231 first limits; 232 Second Edges; 233 the 3rd limits; The height of H1 flow-disturbing part; The liquid that H2 contains the liquid of micro-algae is thick; Adjacent two spacing of streaming the length direction along runner between part of L1; The length of L2 runner; The bearing of trend of X runner; Y first direction.
Embodiment
Below the specific embodiment of the present invention is described in detail.Should be understood that, embodiment described herein, only for instruction and explanation of the present invention, is not limited to the present invention.
The invention provides the method for the micro-algae of a kind of pilot scale culture, under the method is included in the condition of cultivating micro-algae, the cultivation of micro-algae is carried out in bioreactor, as depicted in figs. 1 and 2, this reactor comprises runner 1 and the flow-disturbing part 2 of the liquid-flow allowed containing micro-algae, flow-disturbing part 2 is extended along first direction Y by flow-disturbing cross section 23 and is formed, flow-disturbing cross section 23 comprises and connects into the first close-shaped limit 231 in turn, Second Edge 232 and the 3rd limit 233, first limit 231, Second Edge 232 and the 3rd limit 233 extend along first direction Y and form the first flow-disturbing face 21 respectively, second flow-disturbing face 22 and basal plane, first limit 231 and Second Edge 232 are recessed bent limit, the internal surface that basal plane fits in runner 1 is arranged, first flow-disturbing face 21 and the second flow-disturbing face 22 are arranged relative to basal planes tilted, first direction Y intersects with the bearing of trend X of runner 1.
In the present invention, flow-disturbing part 2 is extended along first direction Y by flow-disturbing cross section 23 and is formed, first direction Y can be one or more in straight line, curve and broken line, but as is known to the person skilled in the art, the cross section of flow-disturbing part 2 on any point of bearing of trend is all vertical with the bearing of trend of this point.
The internal surface that basal plane due to flow-disturbing part 2 fits in runner 1 is arranged, therefore, first direction Y can determine according to the development length of the shape in the cross section of runner 1 and flow-disturbing part 2, namely the cross section of runner 1 refers to the cross section of runner 1 perpendicular to the bearing of trend X of runner 1, when this cross section is rectangle, if flow-disturbing part 2 only extends in the bottom surface of runner 1, then first direction Y is straight line, as shown in (a) in Fig. 3, if flow-disturbing part 2 both extended in the bottom surface of runner 1, extend at the sidewall of runner 1 again, then first direction Y is broken line, as shown in (b) in Fig. 3; When this cross section is the shape of camber line and straight line formation, as shown in (c) in Fig. 3, then first direction Y is curve, specifically, is camber line; When the cross section of the internal surface of runner 1 be base is the rectangle of camber line, as shown in (d) He (e) in Fig. 3, if flow-disturbing part 2 only extends in the bottom surface of runner 1, then first direction is curve, specifically, for camber line, as shown in (d) in Fig. 3, if flow-disturbing part 2 both extended in the bottom surface of runner 1, extend at the sidewall of runner 1 again, then first direction Y is camber line in bottom surface, is straight line, at sidewall as shown in (e) in Fig. 3.Herein in order to clearly describe first direction Y, only list several situation, but not limit, those skilled in the art should be able to be expressly understood first direction Y according to foregoing description.
In the present invention, for the shape in the cross section of runner 1 without particular requirement, can be the thinkable different shape of those skilled in the art, such as, can be the rectangle etc. of camber line for rectangle, semicircle, circle, base.
In the present invention, the first flow-disturbing face 21 and the second flow-disturbing face 22 are arranged relative to basal planes tilted, can towards basal planes tilted, also can basal planes tilted dorsad.Towards basal planes tilted, namely refer to that the angle of the straight flange that the straight flange of the two-end-point on the first limit 231 and each self-forming of two-end-point of Second Edge 232 is formed with the two-end-point on the 3rd limit 233 is respectively less than 90 degree.Basal planes tilted dorsad, namely refers to that the angle of the straight flange that the straight flange of the two-end-point on the first limit 231 or each self-forming of two-end-point of Second Edge 232 and the two-end-point on the 3rd limit 233 are formed is greater than 90 degree.In the present invention, the first flow-disturbing face 21 and the second flow-disturbing face 22 are preferably towards basal planes tilted.
According to the present invention, although the first flow-disturbing face 21 and the second flow-disturbing face 22 are arranged relative to basal planes tilted, object of the present invention can be realized, namely improve the output of micro-algae, the output of micro-algae, preferably towards basal planes tilted, can be improved more in first flow-disturbing face 21 and the second flow-disturbing face 22.But more preferably in situation, when the entrance of the first flow-disturbing face, flow-disturbing face 21 to the second 22 closer to runner 1, the angle of the straight flange that the two-end-point on the straight flange that the two-end-point on the first limit 231 is formed and the 3rd limit 233 is formed is the first angle, the angle of the straight flange that the two-end-point on the straight flange that the two-end-point of Second Edge 232 is formed and the 3rd limit 233 is formed is the second angle, first angle is less than or equal to the second angle, can further improve the output of micro-algae; First angle is 30-60 degree, and the second angle is 45-80 degree, further can improve the output of micro-algae.
In order to give full play to " sparkle effect " of micro-algae, improve the output of micro-algae further, under preferable case, the flow velocity of the liquid-flow containing micro-algae is 0.3-2m/s.Preferably, the first limit 231 has first curvature radius, and Second Edge 232 has second curvature radius, and first curvature radius is more than or equal to second curvature radius; More preferably, first curvature radius is 2-10 times of the height H 1 of flow-disturbing part 2, and second curvature radius is 1-5 times of the height H 1 of flow-disturbing part 2.Under above-mentioned preferable case, further can improve the output of micro-algae.
It will be understood by those skilled in the art that recessed bent limit refers to relative to flow-disturbing cross section 23, the recessed inside to flow-disturbing cross section 23, bent limit, as shown in Figure 4.
In the present invention, for first direction Y, as long as intersect with the bearing of trend X of runner 1, therefore, first direction Y except can not parallel with the bearing of trend X of runner 1 except, can becoming arbitrarily angled with the bearing of trend X of runner 1, playing larger flow-disturbing effect to make flow-disturbing face, under preferable case, first direction Y is vertical with the bearing of trend X of runner 1.
In the present invention, the ratio of the length that the internal surface of the length that flow-disturbing part 2 extends along first direction Y and runner 1 extends along first direction Y is preferably 1:1-10, is more preferably 1:1-5.Under this preferable case, flow-disturbing part 2 can play flow-disturbing effect more fully, can further improve the efficiency of light energy utilization and the photosynthetic efficiency of micro-algae, improves the output of micro-algae further.
For the length that the internal surface of runner 1 extends along first direction Y, multiple situation can be divided into according to first direction Y, be explained as follows: when in such as Fig. 3 shown in (a) time, namely when first direction Y is straight line, namely the internal surface of runner 1 refers to along the length that first direction Y extends the length that the bottom surface of runner 1 extends along first direction Y, when first direction Y is vertical with the bearing of trend X of runner 1, namely the internal surface of runner 1 refers to the width of the bottom surface of runner 1 along the length that first direction Y extends; When in such as Fig. 3 shown in (b) time, namely when first direction Y is broken line, namely the internal surface of runner 1 refers to along the length that first direction Y extends the length that the sidewall that the bottom surface of runner 1 adds upper runner 1 along the length that first direction Y extends extends along first direction Y, when first direction Y is vertical with the bearing of trend X of runner 1, along the length that first direction Y extends, namely the internal surface of runner 1 refers to that the width of the bottom surface of runner 1 adds the height of two sidewalls of upper runner 1; When in such as Fig. 3 shown in (c) and (d) time, namely when first direction Y is curve, namely the internal surface of runner 1 refers to along the length that first direction Y extends the length that the bottom surface of runner 1 extends along first direction Y, when first direction Y is vertical with the bearing of trend X of runner 1, namely the internal surface of runner 1 refers to the arc length of the bottom surface of runner 1 along the length that first direction Y extends; When in such as Fig. 3 shown in (e) time, namely first direction Y is camber line in bottom surface, when sidewall is straight line, namely the internal surface of runner 1 refers to along the length that first direction Y extends the length that the sidewall that the bottom surface of runner 1 adds upper runner 1 along the length that first direction Y extends extends along first direction Y, when first direction Y is vertical with the bearing of trend X of runner 1, along the length that first direction Y extends, namely the internal surface of runner 1 refers to that runner 1 adds the height of two sidewalls of upper runner 1 perpendicular to the arc length on the base in the cross section of the bearing of trend X of runner 1.Herein in order to the length that the internal surface of runner 1 extends along first direction Y is clearly described, only list several situation, but not limit, those skilled in the art should be able to be expressly understood according to foregoing description the length that the internal surface of runner 1 extends along first direction Y.
The ratio of the length that the internal surface that in Fig. 3, (b)-(d) shows length that flow-disturbing part 2 extends along first direction Y and runner 1 extends along first direction Y is the situation of 1:1; The situation that the ratio of the length that the internal surface that in Fig. 3, (a) and (e) show length that flow-disturbing part 2 extends along first direction Y and runner 1 extends along first direction Y is less than 1, a part for the length that the internal surface that namely flow-disturbing part 2 is only runner 1 along the length that first direction Y extends extends along first direction Y.
In the present invention, for the number of flow-disturbing part 2 without particular requirement, in order to play better flow-disturbing effect, flow-disturbing part 2 is preferably multiple, namely more than one.The shape of multiple flow-disturbing part 2 can be the same or different.When flow-disturbing part 2 is multiple, the spacing L1 of the length direction along runner 1 between adjacent two flow-disturbing parts 2 and the ratio of the length L2 of runner 1 are preferably≤0.5.The spacing L1 of the length direction along runner 1 between adjacent two flow-disturbing parts 2 as shown in Figure 1.Under this preferable case, can further improve the efficiency of light energy utilization and the photosynthetic efficiency of micro-algae, improve the output of micro-algae further.
In the present invention, the height H 1 of flow-disturbing part 2 is preferably 1:2-20, more preferably 1:5-15 with the ratio of the thick H2 of liquid of the liquid containing micro-algae.Under this preferable case, flow-disturbing part 2 can play flow-disturbing effect more fully.As previously mentioned, namely the height H 1 of flow-disturbing part 2 refers to the vertical range of the vertex of flow-disturbing part 2 to the basal plane of flow-disturbing part 2.The height H 1 of flow-disturbing part 2 and the thick H2 of liquid of the liquid containing micro-algae are as shown in Figure 1.
In the present invention, the basal plane of flow-disturbing part 2 preferably fits in the not sensitive surface setting of runner 1.Under this preferable case, can further improve the efficiency of light energy utilization and the photosynthetic efficiency of micro-algae, improve the output of micro-algae further.This is because the micro-algae near not sensitive surface can't accept illumination, the basal plane of flow-disturbing part 2 fits in the not sensitive surface setting of runner 1, can make to can't accept the flow-disturbing move under influence of the micro-algae near the not sensitive surface of illumination at flow-disturbing part 2 to illumination place, thus improve the efficiency of light energy utilization and the photosynthetic efficiency of micro-algae in runner 1, improve the output of micro-algae.
In the present invention, the thick H2 of liquid of the liquid containing micro-algae, without particular requirement, can adopt the liquid of this area routine thick, and such as, the thick H2 of liquid of the liquid containing micro-algae can be 1-30cm.
It will be understood by those skilled in the art that micro-algae is flowed in runner under the effect of pump, therefore, in order to reduce the energy consumption of pump, the pitch angle of runner 1 is preferably 0-3 degree.The pitch angle of runner 1 refers to runner 1 angle of inclination with respect to the horizontal plane.
The runner 1 of the bioreactor of the micro-algae of pilot scale culture provided by the invention can be one also can be multiple, can cultivate the amount setting of micro-algae according to reality.
In the present invention, for the wall thickness of runner 1 without particular requirement, the setting of this area routine can be adopted.For the material of runner 1 and flow-disturbing part 2 also without particular requirement, can be one or more in transparent glass, plastics and resin, also can be one or more in opaque stainless steel, aluminium alloy and cement, the material of flow-disturbing part 2 and the material of runner 1 can be the same or different, in order to easily manufactured, the material of preferred flow-disturbing part 2 is identical with the material of runner 1.
In the present invention, flow-disturbing part 2 can be solid construction also can be hollow structure.
In the present invention, for flow-disturbing part 2 at the set-up mode of the internal surface of runner 1 also without particular requirement, the thinkable various mode of those skilled in the art can be adopted, such as, flow-disturbing part 2 and runner 1 can be manufactured an entirety, also flow-disturbing part 2 can be adhered to or is assembled on runner 1.
The bioreactor of the micro-algae of pilot scale culture of the present invention can be Race-way photobioreactor, as shown in (c) in Fig. 3; Also can be closed photo bioreactor, as shown in (a) and (b), (d) He (e) in Fig. 3.
The inventive method is by arranging flow-disturbing part in bioreactor, the output of micro-algae is improved by the cooperation of flow-disturbing part and flow velocity, therefore, for cultivating the condition of micro-algae without particular requirement, the condition that this area is conventional can be adopted, such as, the condition of cultivating micro-algae can comprise: temperature is 15-40 DEG C, is preferably 25-35 DEG C; Light intensity is 2000-30000 lux, is preferably 5000-20000 lux; Pass into the mixed gas of air and carbonic acid gas; The pH value controlling the nutrient solution containing micro-algae is 6-9.
In the present invention, kind for micro-algae has no particular limits, and is preferably oil-producing microalgae, is more preferably the produce oil engineering microalgae with larger industrial utilization, such as, micro-algae can be selected from least one in chlorella, grid algae, spirulina, chrysophyceae and Phaeodactylum tricornutum.
Embodiment
The present invention is further illustrated for following embodiment, but therefore do not limit the present invention.
In the following Examples and Comparative Examples:
Chlorella is purchased from Inst. of Hydrobiology, Chinese Academy of Sciences, and nutrient solution is BG11.
In bioreactor, cultivate micro-algae, all adopt following condition: before inoculation, first carry out disinfection to bioreactor with clorox, then use sterile water wash, interval was inoculated after 12 hours.Nutrient solution is BG11, and with the dry weight basis of chlorella, concentration during chlorella inoculation is 0.13mg/l, and controlling algae liquid temp is 25 DEG C, passes into the mixed gas of air and carbonic acid gas, and the pH value controlling the nutrient solution containing micro-algae is 6-9, and light intensity is 12000 luxs.Regularly supplement the nutrients between incubation period salt in nutrient solution, and add a small amount of water and make that cultivation algae liquid is long-pending keeps constant.Each embodiment is identical with the condition that comparative example cultivates chlorella.
Embodiment 1
The present embodiment is for illustration of the method for the micro-algae of pilot scale culture of the present invention.
Chlorella is cultivated under natural lighting.Bioreactor is by upper and lower two runners 1, liquid-storing box and recycle pump composition, runner 1 is effective plane of illumination region, the long 150cm of each runner 1, wide 10cm, the thick 5cm of liquid, without pitch angle, runner 1 bottom surface is not sensitive surface, the not sensitive surface of each runner 1 is provided with 10 flow-disturbing parts 2, flow-disturbing part 2 is extended along first direction Y by flow-disturbing cross section 23 and is formed, flow-disturbing cross section 23 is formed by connecting into three close-shaped limits in turn, be respectively the first limit 231, Second Edge 232 and the 3rd limit 233, first limit 231 and Second Edge 232 are recessed bent limit, 3rd limit 233 is straight flange, first limit 231, Second Edge 232 and the 3rd limit 233 extend along first direction Y and form the first flow-disturbing face 21 respectively, second flow-disturbing face 22 and basal plane, the internal surface that basal plane fits in runner 1 is arranged, first flow-disturbing face, flow-disturbing face 21 to the second 22 is closer to the entrance of runner 1, the angle on the straight flange that the two-end-point on the first limit 231 is formed and the 3rd limit 233 is the first angle, the angle on the straight flange that the two-end-point of Second Edge 232 is formed and the 3rd limit 233 is the second angle, first angle is 30 degree, second angle is 45 degree, first limit 231 has first curvature radius, Second Edge 232 has second curvature radius, first curvature radius is 6cm, second curvature radius is 3cm.First direction Y is vertical with the bearing of trend X of runner 1, and the ratio of the length that the internal surface of the length that flow-disturbing part 2 extends along first direction Y and runner 1 extends along first direction Y is 1:1.The spacing L1 of the length direction along runner 1 between adjacent two flow-disturbing parts 2 and the ratio of the length L2 of runner 1 are 1:10.The height H 1 of flow-disturbing part 2 is 1:5 with the ratio of the thick H2 of liquid of the liquid containing chlorella.The liquid containing chlorella in liquid-storing box enters in upper runner at the flows by action of recycle pump, enters lower flow channel, flows back to liquid-storing box, Cyclic culture at the flows by action of original speed, and the flow velocity of liquid in runner controlled containing chlorella by recycle pump is 0.3m/s.With the dry weight basis of chlorella, the concentration of different incubation time chlorella is as shown in table 1.
Embodiment 2
The present embodiment is for illustration of the method for the micro-algae of pilot scale culture of the present invention.
Chlorella is cultivated under natural lighting.Bioreactor is by upper and lower two runners 1, liquid-storing box and recycle pump composition, runner 1 is effective plane of illumination region, the long 150cm of each runner 1, wide 10cm, the thick 15cm of liquid, runner pitch angle is 1 degree, runner 1 bottom surface is not sensitive surface, the not sensitive surface of each runner 1 is provided with 10 flow-disturbing parts 2, flow-disturbing part 2 is extended along first direction Y by flow-disturbing cross section 23 and is formed, flow-disturbing cross section 23 is formed by connecting into three close-shaped limits in turn, be respectively the first limit 231, Second Edge 232 and the 3rd limit 233, first limit 231 and Second Edge 232 are recessed bent limit, 3rd limit 233 is straight flange, first limit 231, Second Edge 232 and the 3rd limit 233 extend along first direction Y and form the first flow-disturbing face 21 respectively, second flow-disturbing face 22 and basal plane, the internal surface that basal plane fits in runner 1 is arranged, first flow-disturbing face, flow-disturbing face 21 to the second 22 is closer to the entrance of runner 1, the angle on the straight flange that the two-end-point on the first limit 231 is formed and the 3rd limit 233 is the first angle, the angle on the straight flange that the two-end-point of Second Edge 232 is formed and the 3rd limit 233 is the second angle, first angle is 45 degree, second angle is 75 degree, first limit 231 has first curvature radius, Second Edge 232 has second curvature radius, first curvature radius is 15cm, second curvature radius is 7.5cm.First direction Y is vertical with the bearing of trend X of runner 1, and the ratio of the length that the internal surface of the length that flow-disturbing part 2 extends along first direction Y and runner 1 extends along first direction Y is 1:3.The spacing L1 of the length direction along runner 1 between adjacent two flow-disturbing parts 2 and the ratio of the length L2 of runner 1 are 1:15.The height H 1 of flow-disturbing part 2 is 1:10 with the ratio of the thick H2 of liquid of the liquid containing chlorella.The liquid containing chlorella in liquid-storing box enters in upper runner at the flows by action of recycle pump, enters lower flow channel, flows back to liquid-storing box, Cyclic culture at the flows by action of original speed, and the flow velocity of liquid in runner controlled containing chlorella by recycle pump is 1.2m/s.With the dry weight basis of chlorella, the concentration of different incubation time chlorella is as shown in table 1.
Embodiment 3
The present embodiment is for illustration of the method for the micro-algae of pilot scale culture of the present invention.
Chlorella is cultivated under natural lighting.Bioreactor is by upper and lower two runners 1, liquid-storing box and recycle pump composition, runner 1 is effective plane of illumination region, the long 150cm of each runner 1, wide 10cm, the thick 30cm of liquid, runner pitch angle is 3 degree, runner 1 bottom surface is not sensitive surface, the not sensitive surface of each runner 1 is provided with 10 flow-disturbing parts 2, flow-disturbing part 2 is extended along first direction Y by flow-disturbing cross section 23 and is formed, flow-disturbing cross section 23 is formed by connecting into three close-shaped limits in turn, be respectively the first limit 231, Second Edge 232 and the 3rd limit 233, first limit 231 and Second Edge 232 are recessed bent limit, 3rd limit 233 is straight flange, first limit 231, Second Edge 232 and the 3rd limit 233 extend along first direction Y and form the first flow-disturbing face 21 respectively, second flow-disturbing face 22 and basal plane, the internal surface that basal plane fits in runner 1 is arranged, first flow-disturbing face, flow-disturbing face 21 to the second 22 is closer to the entrance of runner 1, the angle on the straight flange that the two-end-point on the first limit 231 is formed and the 3rd limit 233 is the first angle, the angle on the straight flange that the two-end-point of Second Edge 232 is formed and the 3rd limit 233 is the second angle, first angle is 60 degree, second angle is 80 degree, first limit 231 has first curvature radius, Second Edge 232 has second curvature radius, first curvature radius is 4cm, second curvature radius is 2cm.First direction Y is vertical with the bearing of trend X of runner 1, and the ratio of the length that the internal surface of the length that flow-disturbing part 2 extends along first direction Y and runner 1 extends along first direction Y is 1:5.The spacing L1 of the length direction along runner 1 between adjacent two flow-disturbing parts 2 and the ratio of the length L2 of runner 1 are 1:20.The height H 1 of flow-disturbing part 2 is 1:15 with the ratio of the thick H2 of liquid of the liquid containing chlorella.The liquid containing chlorella in liquid-storing box enters in upper runner at the flows by action of recycle pump, enters lower flow channel, flows back to liquid-storing box, Cyclic culture at the flows by action of original speed, and the flow velocity of liquid in runner controlled containing chlorella by recycle pump is 2m/s.With the dry weight basis of chlorella, the concentration of different incubation time chlorella is as shown in table 1.
Embodiment 4
The present embodiment is for illustration of the method for the micro-algae of pilot scale culture of the present invention.
Chlorella is cultivated according to the method for embodiment 1, unlike, first curvature radius is 12cm, and second curvature radius is 6cm.With the dry weight basis of chlorella, the concentration of different incubation time chlorella is as shown in table 1.
Embodiment 5
The present embodiment is for illustration of the method for the micro-algae of pilot scale culture of the present invention.
Chlorella is cultivated according to the method for embodiment 1, unlike, first curvature radius is 1cm, and second curvature radius is 6cm.With the dry weight basis of chlorella, the concentration of different incubation time chlorella is as shown in table 1.
Embodiment 6
The present embodiment is for illustration of the method for the micro-algae of pilot scale culture of the present invention.
Chlorella is cultivated according to the method for embodiment 1, unlike, the flow velocity of liquid in runner controlled containing chlorella by recycle pump is 2.2m/s.With the dry weight basis of chlorella, the concentration of different incubation time chlorella is as shown in table 1.
Embodiment 7
The present embodiment is for illustration of the method for the micro-algae of pilot scale culture of the present invention.
Chlorella is cultivated according to the method for embodiment 1, unlike, the flow velocity of liquid in runner controlled containing chlorella by recycle pump is 0.2m/s.With the dry weight basis of chlorella, the concentration of different incubation time chlorella is as shown in table 1.
Embodiment 8
The present embodiment is for illustration of the method for the micro-algae of pilot scale culture of the present invention.
Chlorella is cultivated according to the method for embodiment 1, unlike, the first angle is 20 degree, and the second angle is 85 degree.With the dry weight basis of chlorella, the concentration of different incubation time chlorella is as shown in table 1.
Embodiment 9
The present embodiment is for illustration of the method for the micro-algae of pilot scale culture of the present invention.
Chlorella is cultivated according to the method for embodiment 1, unlike, the first angle is 80 degree, and the second angle is 30 degree.With the dry weight basis of chlorella, the concentration of different incubation time chlorella is as shown in table 1.
Comparative example 1
Chlorella is cultivated according to the method for embodiment 1, unlike, flow-disturbing cross section 23 is rectangle, and the 3rd limit 233 be affixed in the limit of not sensitive surface and embodiment 1 is isometric, and another length of side rectangular is equal with the high H1 of embodiment 1 intermediate cam shape.With the dry weight basis of chlorella, the concentration of different incubation time chlorella is as shown in table 1.
Comparative example 2
Chlorella is cultivated according to the method for embodiment 1, unlike, do not add flow-disturbing part in bioreactor.With the dry weight basis of chlorella, the concentration of different incubation time chlorella is as shown in table 1.
Table 1
Embodiment 1-9 is compared can be found out with comparative example 1 and comparative example 2 respectively, and the method for the micro-algae of pilot scale culture of the present invention can improve the output of micro-algae.
Embodiment 1 is compared can be found out with embodiment 4 and embodiment 5 respectively, and the first angle is less than or equal to the second angle, is more conducive to the raising of the output of micro-algae; First angle is 30-60 degree, and the second angle is 45-80 degree, is further conducive to the raising of the output of micro-algae.Embodiment 1 is compared can be found out with embodiment 6 and embodiment 7 respectively, and first curvature radius is more than or equal to second curvature radius, is more conducive to the raising of the output of micro-algae; First curvature radius is 2-10 times of the height H 1 of flow-disturbing part 2, and second curvature radius is 1-5 times of the height H 1 of flow-disturbing part 2, is further conducive to the raising of the output of micro-algae; Embodiment 1 is compared can be found out with embodiment 8 and embodiment 9 respectively, and the flow velocity of the liquid-flow containing micro-algae is 0.3-2m/s, is more conducive to the raising of the output of micro-algae.
The method of the micro-algae of pilot scale culture of the present invention, can increase the motion vertically of micro-algae, makes to shuttle back and forth between Wei Zaoguang district and dark space, thus improves the efficiency of light energy utilization and the photosynthetic efficiency of micro-algae, improve the output of micro-algae.
Below the preferred embodiment of the present invention is described in detail by reference to the accompanying drawings; but; the present invention is not limited to the detail in above-mentioned embodiment; within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
It should be noted that in addition, each concrete technical characteristic described in above-mentioned embodiment, in reconcilable situation, can be combined by any suitable mode, in order to avoid unnecessary repetition, the present invention illustrates no longer separately to various possible array mode.
In addition, also can carry out arbitrary combination between various different embodiment of the present invention, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (15)
1. the method for the micro-algae of pilot scale culture, under described method is included in the condition of cultivating micro-algae, the cultivation of micro-algae is carried out in bioreactor, it is characterized in that, described reactor comprises runner (1) and the flow-disturbing part (2) of the liquid-flow allowed containing micro-algae, described flow-disturbing part (2) is extended along first direction (Y) by flow-disturbing cross section (23) and is formed, described flow-disturbing cross section (23) comprises and connects into close-shaped the first limit (231) in turn, Second Edge (232) and the 3rd limit (233), described first limit (231), described Second Edge (232) and described 3rd limit (233) extend along described first direction (Y) and form the first flow-disturbing face (21) respectively, second flow-disturbing face (22) and basal plane, described first limit (231) and described Second Edge (232) are recessed bent limit, the internal surface that described basal plane fits in described runner (1) is arranged, described first flow-disturbing face (21) and described second flow-disturbing face (22) are arranged relative to described basal planes tilted, described first direction (Y) intersects with the bearing of trend (X) of described runner (1).
2. method according to claim 1, wherein, described first flow-disturbing face (21) and described second flow-disturbing face (22) are towards described basal planes tilted.
3. method according to claim 2, wherein, described first flow-disturbing face (21) is than the entrance of described second flow-disturbing face (22) closer to described runner (1), the angle of the straight flange that the two-end-point on the straight flange that the two-end-point of described first limit (231) is formed and described 3rd limit (233) is formed is the first angle, the angle of the straight flange that the two-end-point on the straight flange that the two-end-point of described Second Edge (232) is formed and described 3rd limit (233) is formed is the second angle, and described first angle is less than or equal to described second angle.
4. method according to claim 3, wherein, described first angle is 30-60 degree, and described second angle is 45-80 degree.
5. method according to claim 4, wherein, the flow velocity of the described liquid-flow containing micro-algae is 0.3-2m/s.
6. method according to claim 5, wherein, described first limit (231) has first curvature radius, and described Second Edge (232) has second curvature radius, and described first curvature radius is more than or equal to described second curvature radius.
7. method according to claim 6, wherein, described first curvature radius is 2-10 times of the height (H1) of flow-disturbing part (2), and described second curvature radius is 1-5 times of the height (H1) of flow-disturbing part (2).
8. according to the method in claim 1-7 described in any one, wherein, described first direction (Y) is vertical with the bearing of trend (X) of described runner (1).
9. the ratio of length that according to the method in claim 1-7 described in any one, wherein, the internal surface of the length that extends along described first direction (Y) of described flow-disturbing part (2) and described runner (1) extends along described first direction (Y) is 1:1-10.
10. according to the method in claim 1-7 described in any one, wherein, described flow-disturbing part (2) for multiple, ratio≤0.5 of the spacing (L1) of the length direction along described runner (1) between adjacent two described flow-disturbing parts (2) and the length (L2) of described runner (1).
11. according to the method in claim 1-7 described in any one, and wherein, the height (H1) of described flow-disturbing part (2) is 1:2-20 with the ratio of the liquid thick (H2) of the described liquid containing micro-algae.
12. according to the method in claim 1-7 described in any one, and wherein, the not sensitive surface that described basal plane fits in described runner (1) is arranged.
13. according to the method in claim 1-7 described in any one, and wherein, the liquid thick (H2) of the described liquid containing micro-algae is 1-30cm.
14. according to the method in claim 1-7 described in any one, and wherein, the pitch angle of described runner (1) is 0-3 degree.
15. according to the method in claim 1-7 described in any one, and wherein, described micro-algae is selected from least one in chlorella, grid algae, spirulina, chrysophyceae and Phaeodactylum tricornutum.
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