CN102787066A - Enclosed perfusion-type photobioreactor - Google Patents
Enclosed perfusion-type photobioreactor Download PDFInfo
- Publication number
- CN102787066A CN102787066A CN2011101307333A CN201110130733A CN102787066A CN 102787066 A CN102787066 A CN 102787066A CN 2011101307333 A CN2011101307333 A CN 2011101307333A CN 201110130733 A CN201110130733 A CN 201110130733A CN 102787066 A CN102787066 A CN 102787066A
- Authority
- CN
- China
- Prior art keywords
- perfusion
- enclosed
- perfusion type
- miniature
- type bioreactor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides an enclosed perfusion-type photobioreactor. The enclosed perfusion-type photobioreactor comprises a bioreactor body. A static mixer is arranged in the bioreactor body. The static mixer is composed of multiple miniature step-type grooves. The enclosed perfusion-type photobioreactor is an all-glass perfusion-type photobioreactor and can be used for suspension culture of algae and other photosynthetic organism cells. The enclosed perfusion-type photobioreactor utilizes the static mixer which is a microstep-type mixer to realize mixing, mass transfer and heat transfer of all organisms in the enclosed perfusion-type photobioreactor, has a simple structure, is convenient for operation, realizes a very high concentration of cultured microalgae, and reduces an alga culture cost.
Description
Technical field
The present invention relates to biochemical technology, specifically, relate to the bioreactor technology field, particularly a kind of closed perfusion type bioreactor.
Background technology
The research of bioreactor starts from the forties in 20th century, and major objective at that time is to carry out a large amount of cultivations of little algae, and main purpose is to inquire into the feasibility of little algae as following food protein of the mankind and fuel source.Bioreactor (photo bioreactor) can be used for photosynthetic microorganism and have the tissue of photosynthetic capacity or one type of device of cell; Have similar structure with general bio-reactor, have the adjusting and the system of culture condition such as light, temperature, dissolved oxygen, carbonic acid gas and nutritive substance.Because bioreactor has higher optical energy utilization efficiency; Under optimum regime, can reach 18%, and plant and forest can only utilize 0.2% of incident optical energy, and can cultured continuously; Therefore, can realize the high-density culture of photosynthetic organism and obtain higher unit surface or volumetric production.
Bioreactor is divided into two kinds of closed and open types, and closed photo bioreactor mainly includes vertical pillar; The level tubular type, vertical or structure level is board-like and four kinds of conventional stirred-tank formula reactor drums etc. of circling round.Because closed have pollution-free, culture condition and be easy to multiple advantages such as control, so use range is wider.But because the structure more complicated of sealing pot type, volume is bigger, and input cost is higher.
Summary of the invention
The defective that technical problem to be solved by this invention is to overcome closed photo bioreactor complex structure in the prior art, is difficult for processing and manufacturing, use in inconvenient laboratory; A kind of higher photosynthetic efficiency, the perfusion type bioreactor that small test chamber simple in structure, easy and simple to handle and stable performance is used are provided.
Another technical problem to be solved of the present invention is to provide the application of a kind of described perfusion type photoreactor in algae and other photosynthetic organism suspension culture of cells.
In order to solve above-mentioned technical problem, technical scheme of the present invention is: a kind of perfusion type bioreactor, comprise a tank body, and be provided with static mixing device in the described tank body.
Further, described static mixing device is made up of a plurality of miniature staged grooves.
The nutrient solution that contains mikrobe is carried from the tank body top through recycle pump, slowly flows through static mixing device, and in process of flowing, nutrient solution receives abundant illumination, carries out photosynthetic response.
Further, described miniature staged groove, the angle of each groove are 60 degree-120 degree.
Further, the angle of described groove is 90 degree, like this can be so that nutrient solution can mix with algae more effectively, and have the time of prolongation fully to carry out photosynthetic response.
Further, the angle of described each miniature staged groove is 90 degree, and regular unified structure helps the manufacturing of industry like this.
Further; The both sides equal in length of described each miniature staged groove, regular unified structure helps the manufacturing of industry like this; Certainly in other embodiments of the invention, can also adopt the static mixing device of unequal-sided miniature staged groove structure.
Further, the length of side length of described each miniature staged groove is 1～2cm.
Further, pump into the nutrient solution flow velocity 5cm-10cm/min of said tank body.In order to guarantee the nutrient solution uniform mixing, little algae can receive full and uniform illumination, guarantees that simultaneously little algae has enough incubation times, and the nutrient solution flow velocity is made as 5cm-10cm/min.
After adopting above design, micro algae growth speed is only reached 14 restrain every day, photosynthetic efficiency reaches more than 8.5%.
Further, described tank body is that full glass is made, so that can make photosynthetic cells carry out photosynthetic response.
A kind of perfusion type bioreactor is used for algae and photosynthetic organism suspension culture of cells.
The outstanding advantage of this reactor drum is; Owing to utilize little staged mixing device, solved the mixing of all mikrobes in the reactor drum, mass transfer and heat transfer problem, and simple in structure; Easy and simple to handle; And can make cultured microalgae class biology reach very high concentration through experiment showed,, reduce the cost of algae culture.
Description of drawings
Fig. 1 is the reactor monolith setting drawing of one embodiment of the invention;
Fig. 2 is the static mixing device structure iron of a preferred embodiment of the present invention.
Wherein, 100 perfusion type bioreactors, 200 recycle pumps
120 static mixing devices, 300 nutrient solutions
130 miniature staged groove 131 staged micro-recesses one sidewalls
132 another sidewalls of staged micro-recesses
Embodiment
In order to make technique means of the present invention, creation characteristic, to reach purpose and effect and be easy to understand and understand, the present invention is done further elaboration below in conjunction with embodiment.
Be depicted as perfusion type bioreactor embodiment of the present invention like Fig. 1,2.The embodiment of Fig. 1; Be provided with static mixing device 120 in the tank body of perfusion type bioreactor 100, contain the nutrient solution 300 of mikrobe, carry from the tank body top through recycle pump 200; Slowly flow through static mixing device 120; In process of flowing, nutrient solution receives abundant illumination, carries out photosynthetic response.
Can see that from the static mixing device synoptic diagram of Fig. 2 static mixing device 120 is made up of a plurality of miniature staged grooves 130.In this preferred embodiment, the angle of groove is 90 degree, like this can be so that nutrient solution can mix with algae more effectively, and have the time of prolongation fully to carry out photosynthetic response.Certainly, among other embodiment of the present invention, the angle of each groove can be between 60 degree-120 degree.
And both sides 131,132 equal in length of each groove; The length of side length of each groove is 1～2cm, and tactical rule is unified, helps the manufacturing of industry; Certainly in other embodiments of the invention, can also adopt the static mixing device of unequal-sided groove structure.
In order to guarantee the nutrient solution uniform mixing, little algae can receive full and uniform illumination, guarantees that simultaneously little algae has enough incubation times, and the nutrient solution flow velocity is made as 5cm-10cm/min.
After adopting above design, micro algae growth speed is only reached 14 restrain every day, photosynthetic efficiency reaches more than 8.5%.
More than show and described ultimate principle of the present invention, principal character and advantage of the present invention.The technician of the industry should understand; The present invention is not restricted to the described embodiments; That describes in the foregoing description and the specification sheets just explains principle of the present invention; The present invention also has various changes and modifications under the prerequisite that does not break away from spirit and scope of the invention, and these variations and improvement all fall in the scope of the invention that requires protection.
Claims (9)
1. a perfusion type bioreactor comprises a tank body, it is characterized in that, is provided with static mixing device in the described tank body.
2. perfusion type bioreactor according to claim 1 is characterized in that, described static mixing device is made up of a plurality of miniature staged grooves.
3. perfusion type bioreactor according to claim 2 is characterized in that, the angle of described miniature staged groove is 60 degree-120 degree.
4. perfusion type bioreactor according to claim 2 is characterized in that, the angle of described miniature staged groove is 90 degree.
5. perfusion type bioreactor according to claim 2 is characterized in that, the angle of described each miniature staged groove is 90 degree.
6. perfusion type bioreactor according to claim 2 is characterized in that, the both sides equal in length of described each miniature staged groove.
7. perfusion type bioreactor according to claim 2 is characterized in that, the length of side length of described each miniature staged groove is 1～2cm.
8. perfusion type bioreactor according to claim 1 is characterized in that, pumping into said jar of intravital nutrient solution flow velocity is 5cm-10cm/min.
9. a perfusion type bioreactor is used for algae and photosynthetic organism suspension culture of cells.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101307333A CN102787066A (en) | 2011-05-20 | 2011-05-20 | Enclosed perfusion-type photobioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101307333A CN102787066A (en) | 2011-05-20 | 2011-05-20 | Enclosed perfusion-type photobioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102787066A true CN102787066A (en) | 2012-11-21 |
Family
ID=47152658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101307333A Pending CN102787066A (en) | 2011-05-20 | 2011-05-20 | Enclosed perfusion-type photobioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102787066A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293673A (en) * | 2013-07-19 | 2015-01-21 | 中国石油化工股份有限公司 | Method for large-scale cultivation of microalgae |
| CN104293674A (en) * | 2013-07-19 | 2015-01-21 | 中国石油化工股份有限公司 | Method for large-scale cultivation of microalgae |
| CN107365689A (en) * | 2017-07-13 | 2017-11-21 | 河海大学 | A kind of staged pesticide degradation bacteria and net dirty carrier loop flowing load device |
| FR3088650A1 (en) * | 2018-11-20 | 2020-05-22 | Constructions Mécaniques Consultants | System and method for absorbing carbon dioxide |
| WO2022169957A1 (en) * | 2021-02-03 | 2022-08-11 | Symbiox, Inc. | Photosynthetic compositions and methods of preparing and using photosynthetic compositions |
-
2011
- 2011-05-20 CN CN2011101307333A patent/CN102787066A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293673A (en) * | 2013-07-19 | 2015-01-21 | 中国石油化工股份有限公司 | Method for large-scale cultivation of microalgae |
| CN104293674A (en) * | 2013-07-19 | 2015-01-21 | 中国石油化工股份有限公司 | Method for large-scale cultivation of microalgae |
| CN104293674B (en) * | 2013-07-19 | 2017-05-24 | 中国石油化工股份有限公司 | Method for large-scale cultivation of microalgae |
| CN104293673B (en) * | 2013-07-19 | 2017-05-24 | 中国石油化工股份有限公司 | Method for large-scale cultivation of microalgae |
| CN107365689A (en) * | 2017-07-13 | 2017-11-21 | 河海大学 | A kind of staged pesticide degradation bacteria and net dirty carrier loop flowing load device |
| FR3088650A1 (en) * | 2018-11-20 | 2020-05-22 | Constructions Mécaniques Consultants | System and method for absorbing carbon dioxide |
| WO2022169957A1 (en) * | 2021-02-03 | 2022-08-11 | Symbiox, Inc. | Photosynthetic compositions and methods of preparing and using photosynthetic compositions |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Sirohi et al. | Design and applications of photobioreactors-a review | |
| Xu et al. | Microalgal bioreactors: challenges and opportunities | |
| Wang et al. | Closed photobioreactors for production of microalgal biomasses | |
| Posten | Design principles of photo‐bioreactors for cultivation of microalgae | |
| AU2007217821B2 (en) | Photobioreactor and uses therefor | |
| KR101148194B1 (en) | Photobioreactor with Transparent Film | |
| Chanquia et al. | Photobioreactors for cultivation and synthesis: Specifications, challenges, and perspectives | |
| CN102827775B (en) | Method for supplementing fermentation raw material by microbial fermentation tail gas CO2 immobilized by microalgae culture | |
| CN101838606A (en) | Airlift loop bioreactor through microalgae photoautotrophic-photoheterotrophic coupling for carbon emission reduction in sewage treatment | |
| CN102382754B (en) | Optical fiber photobioreactor for making full use of 'flash effect' of microalgae | |
| Yan et al. | A novel low-cost thin-film flat plate photobioreactor for microalgae cultivation | |
| Dębowski et al. | Microalgae–cultivation methods | |
| Sero et al. | Biophotonics for improving algal photobioreactor performance: A review | |
| CN102787066A (en) | Enclosed perfusion-type photobioreactor | |
| CN102864067A (en) | Algae culture system | |
| KR20160000206A (en) | Photo-Bioreactor for Cultivation of Photosynthesis Autotrophic Organisms | |
| Ahn et al. | Design of photobioreactor for mass production of microalgae | |
| CN103602579B (en) | Pipeline-type photobioreactor for scale culture of microalgae | |
| CN103525686A (en) | Composite reinforced microalgae photobioreactor based on hollow light pipes | |
| Sun et al. | Recent progress on converting CO2 into microalgal biomass using suspended photobioreactors | |
| CN111763604A (en) | Pig farm fermentation tail liquid continuous treatment system for efficiently culturing coupled microalgae and operation process thereof | |
| Sergejevová et al. | Photobioreactors with internal illumination | |
| CN104450492A (en) | Gradient illumination photobioreactor and application thereof | |
| KR20150128551A (en) | Photo-Bioreactor for Photosynthesis Autotrophic Organisms | |
| KR20160015137A (en) | Light Tube for Photo-Bioreactor for Cultivation of Photosynthesis Autotrophic Organisms |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| DD01 | Delivery of document by public notice |
Addressee: Qu Zuopeng Document name: Notification that Application Deemed to be Withdrawn |
|
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20121121 |