CN104293674B - Method for large-scale cultivation of microalgae - Google Patents
Method for large-scale cultivation of microalgae Download PDFInfo
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- CN104293674B CN104293674B CN201310306168.0A CN201310306168A CN104293674B CN 104293674 B CN104293674 B CN 104293674B CN 201310306168 A CN201310306168 A CN 201310306168A CN 104293674 B CN104293674 B CN 104293674B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
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Abstract
The invention discloses a method for large-scale cultivation of microalgae. The method includes cultivating microalgae in a photobioreactor under microalgae cultivation conditions. The reactor comprises a runner (1) and spoilers (2). The spoilers (2) are formed by extension of turbulence sections (23) along a first direction (Y). Each turbulence section (23) comprises a first side (231), a second side (232) and a third side (233) that are connected in order into a closed shape, and the three sides extend along the first direction (Y) respectively to form a first turbulence surface (21), a second turbulence surface (22) and a base surface. The first side (231) and the second side (232) are concave curved sides, the base surface is attached to the inner surface of the runner (1), the first turbulence surface (21) and the second turbulence surface (22) are arranged obliquely relative to the base surface, and the first direction (Y) crosses with the extension direction (X) of the runner (1). The method provided by the invention can improve the yield of microalgae.
Description
Technical field
The present invention relates to a kind of method of pilot scale culture microalgae.
Background technology
In recent years, microalgae is considered as one of important source material of bioenergy, is the long-term plan for solving future source of energy crisis
Slightly, as the focus of research.At present, the pilot scale culture of microalgae is the bottleneck for restricting the development of microalgae Biological Energy Industry chain, such as
What realizes micro-algae large-scale, the culture of high yield is one of problem demanding prompt solution.
Pass the principal element for just influenceing micro-algae large-scale culture, directly affect the photosynthetic efficiency of microalgae, growth conditions and
The efficiency of light energy utilization.In the R＆D process of microdisk electrode, it is possible to use improve photosynthetic efficiency the characteristics of microalgae photosynthesis, e.g.,
When displacement of the frustule between light area and dark space reaches certain frequency（Usually above 1Hz）, will occur " flash effect "
（Janssen M,Slenders P,Tramper J,Mur L R,Wijffels R.Enzyme Microbial
Technology,2001,29:298-305）, the photosynthetic efficiency of microalgae can be improved.
Microdisk electrode is typically carried out in bioreactor, and bioreactor can be divided into open optical-biological reaction
Device and closed photo bioreactor.
Race-way photobioreactor, with raceway pond as representative, advantage is for construction cost is low, energy consumption is low, easy care；Shortcoming
Predominantly microalgae concentration ratio it is relatively low, by outside environmental elements influenceed greatly, floor space is big, water evaporation quantity is big.Raceway pond photoproduction
Thing reactor, in actual applications algae solution thickness be generally 15-30cm, often promote algae solution to be followed in pond in the way of wheel rotation
Ring, mixing, prevent frustule precipitation adsorption and increase the contact probability of microalgae and light.But in actual applications, raceway pond
Deficiency is gradually displayed, and in primary stage of inoculation, microalgae concentration ratio is relatively low, and light can reach deeper position, and microalgae can be filled
The illumination of foot carries out photosynthesis, and with the growth of microalgae, concentration is increasing, and optical attenuation is serious, although can be with by stirring
The frustule of lower floor is moved to upper strata, but far from microalgae is met to light the need for, hardly resulted in unit volume higher
Biomass.
Closed photo bioreactor, such as tubular type, board-like, pillar etc., advantage are can to improve concentration, the letter of microdisk electrode
Changed the various growth factors of post-production, microalgae and can control, not vulnerable to pollution, good product quality；Deficiency be incubator in itself
High energy consumption in high cost, incubation.Part researcher thinks that closed photo bioreactor is the hair of micro-algae large-scale culture
Exhibition direction, and carried out substantial amounts of research, various closed photo bioreactors are have developed, promote microalgae bioenergy
Development.Tubular type, pillar and board-like bioreactor are typical closed photo bioreactors, be have also developed on this basis
Many novel photo-biological reactors, although overcoming the deficiency of Race-way photobioreactor to a certain degree, but microalgae light
Close efficiency and the efficiency of light energy utilization is still relatively low.
Therefore, no matter for Race-way photobioreactor or closed photo bioreactor, still need at present with it is low into
This mode further improves the yield of microalgae in micro-algae large-scale incubation.
The content of the invention
Product the invention aims to improve open and enclosed bioreactor pilot scale culture microalgae
A kind of amount, there is provided method of pilot scale culture microalgae.
To achieve these goals, the invention provides a kind of method of pilot scale culture microalgae, methods described is included in
Under conditions of culture microalgae, the culture of microalgae is carried out in bioreactor, wherein, the reactor includes allowing containing micro-
The runner and spoiler of the liquid flowing of algae, the spoiler is extended in a first direction and formed by flow-disturbing section, described to disturb
Flow section include be sequentially connected with into close-shaped first while, second while and the 3rd side, described first while, described second while and institute
State first direction extension described in the 3rd edge and form the first flow-disturbing face, the second flow-disturbing face and basal plane, first side and institute respectively
Second is stated while for concave curved, the basal plane fits in the inner surface setting of the runner, the first flow-disturbing face and described second
Flow-disturbing face is set relative to the basal planes tilted, and the first direction intersects with the bearing of trend of the runner.
Preferably, the first flow-disturbing face and second flow-disturbing are facing to the basal planes tilted.
Preferably, the first flow-disturbing face than the second flow-disturbing face closer to the runner entrance, first side
The angle of straight flange that is formed with the two-end-point on the 3rd side of the straight flange that is formed of two-end-point be first angle, described second
While the straight flange that is formed of two-end-point with the described 3rd while the angle of straight flange that is formed of two-end-point be second angle, described
One angle is less than or equal to the second angle.
Preferably, the first angle is 30-60 degree, and the second angle is 45-80 degree.
Preferably, the flow velocity of the liquid flowing containing microalgae is 0.3-2m/s.
Preferably, first side has first curvature radius, and second side has second curvature radius, described first
Radius of curvature is more than or equal to the second curvature radius；It is highly preferred that 2- of the first curvature radius for the height of spoiler
10 times, the second curvature radius is 1-5 times of the height of spoiler.
The method of pilot scale culture microalgae of the invention, by increasing capacitance it is possible to increase microalgae motion vertically, makes microalgae in light
Shuttled between area and dark space, so as to improve the efficiency of light energy utilization and photosynthetic efficiency of microalgae, improve the yield of microalgae.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Brief description of the drawings
Fig. 1 is the schematic elevation view of the bioreactor according to one embodiment of the present invention.
Fig. 2 is the schematic top plan view of bioreactor as shown in Figure 1.
Fig. 3 is five kinds of schematic side views of implementation method of bioreactor of the invention.
Fig. 4 is the schematic diagram in the flow-disturbing section of the spoiler of bioreactor of the invention.
Description of reference numerals
1 runner；2 spoilers；21 first flow-disturbing faces；22 second flow-disturbing faces；23 flow-disturbing sections；231 first sides；232 second
Side；233 the 3rd sides；The height of H1 spoilers；The liquid that H2 contains the liquid of microalgae is thick；The two neighboring edge streams streamed between part of L1
The spacing of the length direction in road；The length of L2 runners；The bearing of trend of X runners；Y first directions.
Specific embodiment
Specific embodiment of the invention is described in detail below.It should be appreciated that described herein specific
Implementation method is merely to illustrate and explain the present invention, and is not intended to limit the invention.
The invention provides a kind of method of pilot scale culture microalgae, the method is included under conditions of culture microalgae,
The culture of microalgae is carried out in bioreactor, as depicted in figs. 1 and 2, the reactor includes allowing the liquid flow containing microalgae
Dynamic runner 1 and spoiler 2, spoiler 2 by flow-disturbing section 23 in the first direction Y extend and formed, flow-disturbing section 23 includes
Close-shaped first is sequentially connected with 231, second 232 and the 3rd side 233, first at 231, second 232 and the 3rd
Y extensions form the first flow-disturbing face 21, the second flow-disturbing face 22 and basal plane respectively in the first direction on side 233, and first at 231 and second
232 is concave curved side, and basal plane fits in the inner surface setting of runner 1, and the first flow-disturbing face 21 and the second flow-disturbing face 22 are relative to basal plane
It is obliquely installed, first direction Y intersects with the bearing of trend X of runner 1.
In the present invention, spoiler 2 by flow-disturbing section 23 in the first direction Y extend and formed, first direction Y can be straight
One or more in line, curve and broken line, but as it is known by the man skilled in the art, spoiler 2 bearing of trend any point
On bearing of trend of the section all with the point it is vertical.
The inner surface setting of runner 1 is fitted in due to the basal plane of spoiler 2, therefore, first direction Y can be according to runner 1
The shape in section and the development length of spoiler 2 determine that the section of runner 1 is the extension for referring to runner 1 perpendicular to runner 1
The section of direction X, when the rectangular cross-section, if spoiler 2 only extends in the bottom surface of runner 1, first direction Y is straight
In line, such as Fig. 3（a）Shown, if spoiler 2 both extends in the bottom surface of runner 1, and side wall in runner 1 extends, then first party
It is broken line to Y, in such as Fig. 3（b）It is shown；When the section is the shape that camber line and straight line are constituted, in such as Fig. 3（c）It is shown, then
One direction Y is curve, is camber line specifically；When the section of the inner surface of runner 1 is the rectangle that base is camber line, such as Fig. 3
In（d）With（e）Shown, if spoiler 2 only extends in the bottom surface of runner 1, first direction is curve, is arc specifically
In line, such as Fig. 3（d）Shown, if spoiler 2 both extends in the bottom surface of runner 1, and side wall in runner 1 extends, then first party
To Y bottom surface be camber line, in side wall be straight line, in such as Fig. 3（e）It is shown.Here for first direction Y is explicitly described, only arrange
Several situations, but not limit are lifted, those skilled in the art should be able to be expressly understood first party according to foregoing description
To Y.
In the present invention, for runner 1 section shape without particular/special requirement, can for those skilled in the art institute it is contemplated that
It is variously-shaped, for example, can be that rectangle, semicircle, circle, base are rectangle etc. of camber line.
In the present invention, the first flow-disturbing face 21 and the second flow-disturbing face 22 are set relative to basal planes tilted, can incline towards basal plane
Tiltedly, it is also possible to dorsad basal planes tilted.Towards basal planes tilted, that is, refer to first in 231 two-end-point and second 232 two-end-point
The angle of the straight flange that the straight flange of each self-forming is formed with the two-end-point on the 3rd side 233 respectively is less than 90 degree.Dorsad basal planes tilted, i.e.,
Refer to first in 231 two-end-point or second straight flange of 232 each self-forming of two-end-point formed with the two-end-point on the 3rd side 233
Straight flange angle be more than 90 degree.In the present invention, the first flow-disturbing face 21 and the second flow-disturbing face 22 are preferably directed towards basal planes tilted.
According to the present invention, although the first flow-disturbing face 21 and the second flow-disturbing face 22 are set relative to basal planes tilted, you can realize
The purpose of the present invention, that is, improve the yield of microalgae, and the first flow-disturbing face 21 and the second flow-disturbing face 22 are preferably directed towards basal planes tilted, can be more
Plus improve the yield of microalgae.But in the case of more preferably, when the flow-disturbing face 22 of the first flow-disturbing face 21 to the second closer to the entrance of runner 1
When, first in 231 straight flange that is formed of two-end-point and the 3rd angle of the straight flange that 233 two-end-point is formed be first jiao
Degree, second in 232 straight flange that is formed of two-end-point and the 3rd angle of the straight flange that 233 two-end-point is formed be second jiao
Degree, first angle is less than or equal to second angle, can further improve the yield of microalgae；First angle is 30-60 degree, second angle
It is 45-80 degree, can further improves the yield of microalgae.
In order to give full play to " flash effect " of microalgae, the yield of microalgae is further improved, under preferable case, contain microalgae
Liquid flowing flow velocity be 0.3-2m/s.Preferably, the first side 231 has first curvature radius, and the second side 232 has second
Radius of curvature, first curvature radius is more than or equal to second curvature radius；It is highly preferred that height of the first curvature radius for spoiler 2
2-10 times of degree H1, second curvature radius is 1-5 times of the height H1 of spoiler 2.Under above-mentioned preferable case, can be further
Improve the yield of microalgae.
It will be understood by those skilled in the art that concave curved side refers to that for flow-disturbing section 23 curl concaves towards flow-disturbing
The inside in section 23, as shown in Figure 4.
In the present invention, for first direction Y, if intersect with the bearing of trend X of runner 1, therefore, first direction Y
Except can not it is parallel with the bearing of trend X of runner 1 in addition to, can be with the bearing of trend X of runner 1 into arbitrarily angled, in order that flow-disturbing
Face plays bigger flow-disturbing effect, and under preferable case, first direction Y is vertical with the bearing of trend X of runner 1.
In the present invention, Y extends the inner surface of spoiler 2 length and runner 1 that Y extends in the first direction in the first direction
The ratio of length is preferably 1：1-10, more preferably 1：1-5.Under the preferable case, spoiler 2 can be played more fully
Flow-disturbing effect, can further improve the efficiency of light energy utilization and photosynthetic efficiency of microalgae, further improve the yield of microalgae.
Can be various situations, solution according to first direction Y points for the inner surface length that Y extends in the first direction of runner 1
Release as follows：When in such as Fig. 3（a）When shown, i.e. when first direction Y is straight line, Y extends in the first direction for the inner surface of runner 1
Length be refer to runner 1 bottom surface in the first direction Y extend length, when first direction Y is vertical with the bearing of trend X of runner 1
When, the inner surface length that Y extends in the first direction of runner 1 is the width of the bottom surface for referring to runner 1；When in such as Fig. 3（b）It is shown
When, i.e. when first direction Y is broken line, the inner surface of runner 1 length that Y extends in the first direction be refer to runner 1 bottom surface edge
The length that first direction Y extends adds the side wall length that Y extends in the first direction of runner 1, when first direction Y and runner 1
When bearing of trend X is vertical, the inner surface of runner 1 length that Y extends in the first direction is that the width of the bottom surface for referring to runner 1 is added
Two height of side wall of runner 1；When in such as Fig. 3（c）With（d）When shown, i.e. when first direction Y is curve, the interior table of runner 1
Face in the first direction Y extend length be refer to runner 1 bottom surface in the first direction Y extend length, when first direction Y with stream
When the bearing of trend X in road 1 is vertical, the inner surface length that Y extends in the first direction of runner 1 is the arc of the bottom surface for referring to runner 1
It is long；When in such as Fig. 3（e）When shown, i.e. first direction Y is camber line in bottom surface, when in side, wall is straight line, the inner surface edge of runner 1
The length that first direction Y extends be refer to runner 1 the bottom surface length that Y extends in the first direction plus runner 1 side wall along the
The length that one direction Y extends, when first direction Y is vertical with the bearing of trend X of runner 1, the inner surface of runner 1 is in the first direction
The length that Y extends is refer to runner 1 perpendicular to the base in the section of the bearing of trend X of runner 1 arc length plus two of runner 1
The height of side wall.Here for the inner surface length that Y extends in the first direction for clearly demonstrating runner 1, only list several
Situation, but not limit, those skilled in the art should be able to be expressly understood the inner surface edge of runner 1 according to foregoing description
The length that first direction Y extends.
In Fig. 3（b）-（d）Show the length that Y extends in the first direction of spoiler 2 with the inner surface of runner 1 along first party
The ratio of the length extended to Y is 1：1 situation；In Fig. 3（a）With（e）Show the length that Y extends in the first direction of spoiler 2
The situation of the ratio less than 1 of degree and the inner surface of runner 1 length that Y extends in the first direction, i.e. spoiler 2 Y in the first direction
The length of extension be only runner 1 inner surface in the first direction Y extend length a part.
In the present invention, for spoiler 2 number without particular/special requirement, in order to play more preferable flow-disturbing effect, spoiler 2 is excellent
Elect multiple as, i.e., it is more than one.The shape of multiple spoilers 2 can be the same or different.When spoiler 2 is for multiple, phase
The ratio of the spacing L1 and the length L2 of runner 1 of the length direction along runner 1 between adjacent two spoilers 2 is preferably≤0.5.Phase
The spacing L1 of the length direction along runner 1 between adjacent two spoilers 2 is as shown in Figure 1.Under the preferable case, can be further
The efficiency of light energy utilization and photosynthetic efficiency of microalgae are improved, the yield of microalgae is further improved.
In the present invention, the ratio of the height H1 of the spoiler 2 and liquid thickness H2 of the liquid containing microalgae is preferably 1：2-20, enters
One step is preferably 1：5-15.Under the preferable case, spoiler 2 can play more fully flow-disturbing effect.As it was previously stated, flow-disturbing
It refers to the peak of spoiler 2 to the vertical range of the basal plane of spoiler 2 that the height H1 of part 2 is.The height H1 of spoiler 2 with contain
There is the liquid thickness H2 of the liquid of microalgae as shown in Figure 1.
In the present invention, the basal plane of spoiler 2 preferably fits in the not smooth surface setting of runner 1.Under the preferable case, can
The efficiency of light energy utilization and photosynthetic efficiency of microalgae are further improved, the yield of microalgae is further improved.This is because smooth surface is not attached
Near microalgae can't accept illumination, and the basal plane of spoiler 2 fits in the not smooth surface setting of runner 1, can make to can't accept illumination
Not smooth surface near microalgae spoiler 2 flow-disturbing effect under move at illumination, so as to improve microalgae in runner 1
The efficiency of light energy utilization and photosynthetic efficiency, improve the yield of microalgae.
In the present invention, the liquid thickness H2 of the liquid containing microalgae, can be thick using the conventional liquid in this area without particular/special requirement, example
Such as, the liquid thickness H2 of the liquid containing microalgae can be 1-30cm.
It will be understood by those skilled in the art that microalgae is flowed in runner in the presence of pump, therefore, in order to
The energy consumption of pump is reduced, the inclination angle of runner 1 is preferably 0-3 degree.The inclination angle of runner 1 refers to runner 1 with respect to the horizontal plane
Angle of inclination.
It can also be many that the runner 1 of the bioreactor of the pilot scale culture microalgae that the present invention is provided can be one
It is individual, can be according to the amount setting of actual culture microalgae.
In the present invention, for runner 1 wall thickness without particular/special requirement, can be using the conventional setting in this area.For runner 1
Can be one or more in transparent glass, plastics and resin with the material of spoiler 2 also without particular/special requirement, it is also possible to
It it is one or more in opaque stainless steel, aluminium alloy and cement, the material of spoiler 2 and the material of runner 1 can phases
With can also be different, in order to easily manufactured, preferably the material of spoiler 2 be identical with the material of runner 1.
In the present invention, spoiler 2 can also be able to be hollow structure for solid construction.
In the present invention, for spoiler 2 the inner surface of runner 1 set-up mode also without particular/special requirement, can using this
The thinkable various modes of art personnel institute, for example, spoiler 2 and runner 1 can be formed integrally as, it is also possible to
Spoiler 2 is adhered to or is assembled on runner 1.
The bioreactor of pilot scale culture microalgae of the invention can be Race-way photobioreactor, in such as Fig. 3
（c）It is shown；Can also be closed photo bioreactor, in such as Fig. 3（a）、（b）、（d）With（e）It is shown.
The inventive method is, by setting spoiler in bioreactor, to be carried by the cooperation of spoiler and flow velocity
The yield of microalgae high, therefore, for cultivating the condition of microalgae without particular/special requirement, condition commonly used in the art can be used, for example,
The condition for cultivating microalgae can include：Temperature is 15-40 DEG C, preferably 25-35 DEG C；Light intensity is 2000-30000 luxs, excellent
Elect 5000-20000 luxs as；It is passed through the mixed gas of air and carbon dioxide；Control the pH value of the nutrient solution containing microalgae
It is 6-9.
In the present invention, the species for microalgae has no particular limits, preferably oil-producing microalgae, more preferably with larger
Industrial utilization oil-producing engineering microalgae, for example, microalgae can be selected from chlorella, grid algae, spirulina, chrysophyceae and phaeodactylum tricornutum
Refer at least one in algae.
Embodiment
The present invention is further illustrated for following embodiment, but and is not so limited the present invention.
In the following Examples and Comparative Examples：
Chlorella is purchased from Inst. of Hydrobiology, Chinese Academy of Sciences, and nutrient solution is BG11.
Microalgae is cultivated in bioreactor, using following condition：It is first anti-to photo-biological with sodium hypochlorite before inoculation
Device is answered to carry out disinfection, then with sterile water wash, interval is inoculated with after 12 hours.Nutrient solution is BG11, in terms of the dry weight of chlorella,
Concentration when chlorella is inoculated with is 0.13mg/l, controls algae solution temperature for 25 DEG C, is passed through the mixed gas of air and carbon dioxide,
The pH value of the nutrient solution containing microalgae is controlled for 6-9, light intensity is 12000 luxs.Periodically supplemented in nutrient solution during culture
Nutritive salt, and adding a small amount of water makes culture algae solution volume keep constant.Each embodiment and the condition of comparative example culture chlorella
It is identical.
Embodiment 1
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
Chlorella is cultivated under natural lighting.Bioreactor is by upper and lower two runners 1, liquid reserve tank and circulation pump group
Into runner 1 is effective plane of illumination region, each runner 1 150cm long, 10cm wide, liquid thickness 5cm, without inclination angle, the bottom surface of runner 1
It is not smooth surface, is provided with 10 spoilers 2 on the not smooth surface of each runner 1, spoiler 2 is by flow-disturbing section 23 along the
One direction Y extends and is formed, and flow-disturbing section 23 is constituted by being sequentially connected with into three close-shaped sides, respectively the first side 231,
Second at 232 and the 3rd 233, and first at 231 and second 232 is concave curved side, and the 3rd side 233 is straight flange, the first side 231,
Second at 232 and the 3rd 233 in the first direction Y extend form the first flow-disturbing face 21, the second flow-disturbing face 22 and basal plane, base respectively
Face fits in the inner surface setting of runner 1, the flow-disturbing face 22 of the first flow-disturbing face 21 to the second closer to runner 1 entrance, the first side
The straight flange and the angle on the 3rd side 233 that 231 two-end-point is formed are first angle, what the two-end-point on the second side 232 was formed
The angle on straight flange and the 3rd side 233 is second angle, and first angle is 30 degree, and second angle is 45 degree, and the first side 231 is with the
One radius of curvature, the second side 232 has second curvature radius, and first curvature radius is 6cm, and second curvature radius is 3cm.First
Direction Y is vertical with the bearing of trend X of runner 1, and the inner surface of the length that Y extends in the first direction of spoiler 2 and runner 1 is along first
The ratio of the length that direction Y extends is 1：1.The spacing L1 of the length direction along runner 1 between two neighboring spoiler 2 and stream
The ratio of the length L2 in road 1 is 1：10.The ratio of the height H1 of the spoiler 2 and liquid thickness H2 of the liquid containing chlorella is 1：5.
During the liquid containing chlorella in liquid reserve tank flows into upper runner in the presence of circulating pump, flowed into the presence of initial velocity
Lower flow channel, flows back to liquid reserve tank, Cyclic culture, controls flow velocity of the liquid containing chlorella in runner to be by circulating pump
0.3m/s.In terms of the dry weight of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Embodiment 2
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
Chlorella is cultivated under natural lighting.Bioreactor is by upper and lower two runners 1, liquid reserve tank and circulation pump group
Into, runner 1 is effective plane of illumination region, each runner 1 150cm long, 10cm wide, liquid thickness 15cm, and runner inclination angle is 1 degree,
The bottom surface of runner 1 is not smooth surface, and 10 spoilers 2 are provided with the not smooth surface of each runner 1, and spoiler 2 passes through flow-disturbing
Y extends and is formed in the first direction in section 23, and flow-disturbing section 23 is constituted by being sequentially connected with into three close-shaped sides, respectively
First at 231, second 232 and the 3rd side 233, and first at 231 and second 232 is concave curved side, and the 3rd side 233 is straight flange,
First at 231, second 232 and the 3rd side 233 in the first direction Y extend form the first flow-disturbing face 21, the second flow-disturbing face respectively
22 and basal plane, basal plane fits in the inner surface setting of runner 1, and the flow-disturbing face 22 of the first flow-disturbing face 21 to the second is closer to runner 1
Entrance, first in 231 straight flange that is formed of two-end-point and the 3rd 233 angle be first angle, the two ends on the second side 232
The angle on the formed straight flange of point and the 3rd side 233 is second angle, and first angle is 45 degree, and second angle is 75 degree, first
Side 231 has first curvature radius, and the second side 232 has second curvature radius, and first curvature radius is 15cm, torsion half
Footpath is 7.5cm.First direction Y is vertical with the bearing of trend X of runner 1, spoiler 2 Y extends in the first direction length and runner 1
Inner surface in the first direction Y extend length ratio be 1：3.The length side along runner 1 between two neighboring spoiler 2
To the ratio of length L2 of spacing L1 and runner 1 be 1：15.The height H1 of spoiler 2 is thick with the liquid of the liquid containing chlorella
The ratio of H2 is 1：10.During the liquid containing chlorella in liquid reserve tank flows into upper runner in the presence of circulating pump, initial
Lower flow channel is flowed into the presence of speed, liquid reserve tank is flowed back to, Cyclic culture controls the liquid containing chlorella in stream by circulating pump
Flow velocity in road is 1.2m/s.In terms of the dry weight of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Embodiment 3
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
Chlorella is cultivated under natural lighting.Bioreactor is by upper and lower two runners 1, liquid reserve tank and circulation pump group
Into, runner 1 is effective plane of illumination region, each runner 1 150cm long, 10cm wide, liquid thickness 30cm, and runner inclination angle is 3 degree,
The bottom surface of runner 1 is not smooth surface, and 10 spoilers 2 are provided with the not smooth surface of each runner 1, and spoiler 2 passes through flow-disturbing
Y extends and is formed in the first direction in section 23, and flow-disturbing section 23 is constituted by being sequentially connected with into three close-shaped sides, respectively
First at 231, second 232 and the 3rd side 233, and first at 231 and second 232 is concave curved side, and the 3rd side 233 is straight flange,
First at 231, second 232 and the 3rd side 233 in the first direction Y extend form the first flow-disturbing face 21, the second flow-disturbing face respectively
22 and basal plane, basal plane fits in the inner surface setting of runner 1, and the flow-disturbing face 22 of the first flow-disturbing face 21 to the second is closer to runner 1
Entrance, first in 231 straight flange that is formed of two-end-point and the 3rd 233 angle be first angle, the two ends on the second side 232
The angle on the formed straight flange of point and the 3rd side 233 is second angle, and first angle is 60 degree, and second angle is 80 degree, first
Side 231 has first curvature radius, and the second side 232 has second curvature radius, and first curvature radius is 4cm, torsion half
Footpath is 2cm.First direction Y is vertical with the bearing of trend X of runner 1, spoiler 2 Y extends in the first direction length and runner 1
Inner surface in the first direction Y extend length ratio be 1：5.The length direction along runner 1 between two neighboring spoiler 2
The ratio of length L2 of spacing L1 and runner 1 be 1：20.The height H1 of the spoiler 2 and liquid thickness H2 of the liquid containing chlorella
Ratio be 1：15.During the liquid containing chlorella in liquid reserve tank flows into upper runner in the presence of circulating pump, in initial speed
Lower flow channel is flowed into the presence of degree, liquid reserve tank is flowed back to, Cyclic culture controls the liquid containing chlorella in runner by circulating pump
In flow velocity be 2m/s.In terms of the dry weight of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Embodiment 4
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
According to the method culture chlorella of embodiment 1, unlike, first curvature radius is 12cm, second curvature radius
It is 6cm.In terms of the dry weight of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Embodiment 5
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
According to the method culture chlorella of embodiment 1, unlike, first curvature radius is 1cm, and second curvature radius is
6cm.In terms of the dry weight of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Embodiment 6
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
According to the method culture chlorella of embodiment 1, unlike, control the liquid containing chlorella to exist by circulating pump
Flow velocity in runner is 2.2m/s.In terms of the dry weight of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Embodiment 7
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
According to the method culture chlorella of embodiment 1, unlike, control the liquid containing chlorella to exist by circulating pump
Flow velocity in runner is 0.2m/s.In terms of the dry weight of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Embodiment 8
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
According to the method culture chlorella of embodiment 1, unlike, first angle is 20 degree, and second angle is 85 degree.With
The dry weight meter of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Embodiment 9
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
According to the method culture chlorella of embodiment 1, unlike, first angle is 80 degree, and second angle is 30 degree.With
The dry weight meter of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Comparative example 1
According to the method culture chlorella of embodiment 1, unlike, flow-disturbing section 23 is rectangle, is affixed on not smooth surface
While in embodiment 1 the 3rd while it is 233 isometric, rectangular another length of side is equal with the H1 high of the intermediate cam shape of embodiment 1.With
The dry weight meter of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Comparative example 2
According to the method culture chlorella of embodiment 1, unlike, spoiler is not added with bioreactor.With small
The dry weight meter of ball algae, the concentration of different incubation time chlorellas is as shown in table 1.
Table 1
Embodiment 1-9 is compared as can be seen that pilot scale culture of the invention with comparative example 1 and comparative example 2 respectively
The method of microalgae can improve the yield of microalgae.
Embodiment 1 is compared with embodiment 4 and embodiment 5 respectively as can be seen that first angle is less than or equal to second
Angle, is more beneficial for the raising of the yield of microalgae；First angle is 30-60 degree, and second angle is 45-80 degree, is further had
Beneficial to the raising of the yield of microalgae.Embodiment 1 is compared as can be seen that first curvature with embodiment 6 and embodiment 7 respectively
Radius is more than or equal to second curvature radius, is more beneficial for the raising of the yield of microalgae；First curvature radius is the height of spoiler 2
2-10 times of H1, second curvature radius is 1-5 times of height H1 of spoiler 2, is further conducive to the yield of microalgae to carry
It is high；Embodiment 1 is compared with embodiment 8 and embodiment 9 respectively as can be seen that the flow velocity of the liquid flowing containing microalgae is
0.3-2m/s, is more beneficial for the raising of the yield of microalgae.
The method of pilot scale culture microalgae of the invention, by increasing capacitance it is possible to increase microalgae motion vertically, makes microalgae in light
Shuttled between area and dark space, so as to improve the efficiency of light energy utilization and photosynthetic efficiency of microalgae, improve the yield of microalgae.
The preferred embodiment of the present invention is described in detail above in association with accompanying drawing, but, the present invention is not limited to above-mentioned reality
The detail in mode is applied, in range of the technology design of the invention, various letters can be carried out to technical scheme
Monotropic type, these simple variants belong to protection scope of the present invention.
It is further to note that each particular technique feature described in above-mentioned specific embodiment, in not lance
In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the present invention to it is various can
The combination of energy is no longer separately illustrated.
Additionally, can also be combined between a variety of implementation methods of the invention, as long as it is without prejudice to originally
The thought of invention, it should equally be considered as content disclosed in this invention.
Claims (12)
1. a kind of method of pilot scale culture microalgae, methods described is included under conditions of culture microalgae, in bioreactor
In carry out the culture of microalgae, it is characterised in that the reactor includes the runner (1) for allowing the liquid containing microalgae to flow and disturbs
Stream part (2), the spoiler (2) by flow-disturbing section (23) in the first direction (Y) extend and formed, the flow-disturbing section (23)
Including being sequentially connected with into close-shaped first (232) and the 3rd side (233) in (231), second, first side (231),
Described second at (232) and the described 3rd (233) along the first direction (Y) extend formed respectively the first flow-disturbing face (21),
Second flow-disturbing face (22) and basal plane, described first at (231) and described second (232) be concave curved side, the basal plane is fitted in
The inner surface setting of the runner (1), the first flow-disturbing face (21) and the second flow-disturbing face (22) are relative to the basal plane
It is obliquely installed, the first direction (Y) is vertical with the bearing of trend (X) of the runner (1)；
Wherein, the first flow-disturbing face (21) and the second flow-disturbing face (22) are towards the basal planes tilted；
Wherein, the first flow-disturbing face (21) than the second flow-disturbing face (22) closer to the runner (1) entrance, described
The angle of the straight flange that the straight flange that the two-end-point of (231) is formed on one side is formed with the two-end-point of the 3rd side (233) is
One angle, described second when the straight flange that the two-end-point of (232) is formed is with the described 3rd the two-end-point of (233) formed it is straight
The angle on side is second angle, and the first angle is less than or equal to the second angle.
2. method according to claim 1, wherein, the first angle is 30-60 degree, and the second angle is 45-80
Degree.
3. method according to claim 2, wherein, the flow velocity of the liquid flowing containing microalgae is 0.3-2m/s.
4. method according to claim 3, wherein, first side (231) with first curvature radius, second side
(232) with second curvature radius, the first curvature radius is more than or equal to the second curvature radius.
5. method according to claim 4, wherein, the first curvature radius is the 2- of the height (H1) of spoiler (2)
10 times, the second curvature radius is 1-5 times of the height (H1) of spoiler (2).
6. the method according to any one in claim 1-5, wherein, the spoiler (2) is along the first direction (Y)
The ratio of the length that the length of extension extends with the inner surface of the runner (1) along the first direction (Y) is 1：1-10.
7. the method according to any one in claim 1-5, wherein, the spoiler (2) is multiple, two neighboring institute
State the spacing (L1) of the length direction along the runner (1) between spoiler (2) and the ratio of the length (L2) of the runner (1)
Value≤0.5.
8. the method according to any one in claim 1-5, wherein, the height (H1) of the spoiler (2) with it is described
The ratio of liquid thickness (H2) of the liquid containing microalgae is 1：2-20.
9. the method according to any one in claim 1-5, wherein, the basal plane fits in the runner (1) no
Smooth surface is set.
10. the method according to any one in claim 1-5, wherein, liquid thickness (H2) of the liquid containing microalgae
It is 1-30cm.
11. method according to any one in claim 1-5, wherein, the inclination angle of the runner (1) is 0-3 degree.
12. method according to any one in claim 1-5, wherein, the microalgae is selected from chlorella, grid algae, spiral
At least one in algae, chrysophyceae and Phaeodactylum tricornutum.
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| CN101899385A (en) * | 2009-05-27 | 2010-12-01 | 中国科学院过程工程研究所 | Closed photo bioreactor for enhancing light energy utilization rate of microalgae mass culture |
| CN102260629A (en) * | 2010-05-28 | 2011-11-30 | 中国科学院过程工程研究所 | Plate-type photobioreactor |
| CN102787066A (en) * | 2011-05-20 | 2012-11-21 | 曲作鹏 | Enclosed perfusion-type photobioreactor |
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| CN101899385A (en) * | 2009-05-27 | 2010-12-01 | 中国科学院过程工程研究所 | Closed photo bioreactor for enhancing light energy utilization rate of microalgae mass culture |
| CN102260629A (en) * | 2010-05-28 | 2011-11-30 | 中国科学院过程工程研究所 | Plate-type photobioreactor |
| CN102787066A (en) * | 2011-05-20 | 2012-11-21 | 曲作鹏 | Enclosed perfusion-type photobioreactor |
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