CN104293673B - Method for large-scale cultivation of microalgae - Google Patents
Method for large-scale cultivation of microalgae Download PDFInfo
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- CN104293673B CN104293673B CN201310305853.1A CN201310305853A CN104293673B CN 104293673 B CN104293673 B CN 104293673B CN 201310305853 A CN201310305853 A CN 201310305853A CN 104293673 B CN104293673 B CN 104293673B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a method for large-scale cultivation of microalgae. The method includes cultivating microalgae in a photobioreactor under microalgae cultivation conditions. The reactor comprises a runner (1) and spoilers (2). The spoilers (2) are formed by extension of turbulence sections (23) along a first direction (Y). Each turbulence section (23) comprises a first side (231), a second side (232) and a third side (233) that are connected in order into a closed shape, and the three sides extend along the first direction (Y) respectively to form a first turbulence surface (21), a second turbulence surface (22) and a base surface. The first side (231) and the second side (232) are straight sides, the base surface is attached to the inner surface of the runner (1), the first turbulence surface (21) and the second turbulence surface (22) are arranged obliquely relative to the base surface, and the first direction (Y) crosses with the extension direction (X) of the runner (1). The method provided by the invention can improve the yield of microalgae.
Description
Technical field
The present invention relates to a kind of method of pilot scale culture microalgae.
Background technology
In recent years, microalgae is considered as one of important source material of bioenergy, is the long-term plan for solving future source of energy crisis
Slightly, as the focus of research.At present, the pilot scale culture of microalgae is the bottleneck for restricting the development of microalgae Biological Energy Industry chain, such as
What realizes micro-algae large-scale, the culture of high yield is one of problem demanding prompt solution.
Pass the principal element for just influenceing micro-algae large-scale culture, directly affect the photosynthetic efficiency of microalgae, growth conditions and
The efficiency of light energy utilization.In the R＆D process of microdisk electrode, it is possible to use improve photosynthetic efficiency the characteristics of microalgae photosynthesis, e.g.,
When displacement of the frustule between light area and dark space reaches certain frequency（Usually above 1Hz）, will occur " flash effect "
（Janssen M,Slenders P,Tramper J,Mur L R,Wijffels R.Enzyme Microbial
Technology,2001,29:298-305）, the photosynthetic efficiency of microalgae can be improved.
Microdisk electrode is typically carried out in bioreactor, and bioreactor can be divided into open optical-biological reaction
Device and closed photo bioreactor.
Race-way photobioreactor, with raceway pond as representative, advantage is for construction cost is low, energy consumption is low, easy care；Shortcoming
Predominantly microalgae concentration ratio it is relatively low, by outside environmental elements influenceed greatly, floor space is big, water evaporation quantity is big.Raceway pond photoproduction
Thing reactor, in actual applications algae solution thickness be generally 15-30cm, often promote algae solution to be followed in pond in the way of wheel rotation
Ring, mixing, prevent frustule precipitation adsorption and increase the contact probability of microalgae and light.But in actual applications, raceway pond
Deficiency is gradually displayed, and in primary stage of inoculation, microalgae concentration ratio is relatively low, and light can reach deeper position, and microalgae can be filled
The illumination of foot carries out photosynthesis, and with the growth of microalgae, concentration is increasing, and optical attenuation is serious, although can be with by stirring
The frustule of lower floor is moved to upper strata, but far from microalgae is met to light the need for, hardly resulted in unit volume higher
Biomass.
Closed photo bioreactor, such as tubular type, board-like, pillar etc., advantage are can to improve concentration, the letter of microdisk electrode
Changed the various growth factors of post-production, microalgae and can control, not vulnerable to pollution, good product quality；Deficiency be incubator in itself
High energy consumption in high cost, incubation.Part researcher thinks that closed photo bioreactor is the hair of micro-algae large-scale culture
Exhibition direction, and carried out substantial amounts of research, various closed photo bioreactors are have developed, promote microalgae bioenergy
Development.Tubular type, pillar and board-like bioreactor are typical closed photo bioreactors, be have also developed on this basis
Many novel photo-biological reactors, although overcoming the deficiency of Race-way photobioreactor to a certain degree, but microalgae light
Close efficiency and the efficiency of light energy utilization is still relatively low.
Therefore, no matter for Race-way photobioreactor or closed photo bioreactor, still need at present with it is low into
This mode further improves the yield of microalgae in micro-algae large-scale incubation.
The content of the invention
Product the invention aims to improve open and enclosed bioreactor pilot scale culture microalgae
A kind of amount, there is provided method of pilot scale culture microalgae.
To achieve these goals, the invention provides a kind of method of pilot scale culture microalgae, methods described is included in
Under conditions of culture microalgae, the culture of microalgae is carried out in bioreactor, wherein, the reactor includes allowing containing micro-
The runner and spoiler of the liquid flowing of algae, the spoiler is extended in a first direction and formed by flow-disturbing section, described to disturb
Flow section include be sequentially connected with into close-shaped first while, second while and the 3rd side, described first while, described second while and institute
State first direction extension described in the 3rd edge and form the first flow-disturbing face, the second flow-disturbing face and basal plane, first side and institute respectively
The second side is stated for straight flange, the basal plane fits in the inner surface setting of the runner, and the first flow-disturbing face and described second disturb
Stream interface is set relative to the basal planes tilted, and the first direction intersects with the bearing of trend of the runner.
Preferably, the first flow-disturbing face and second flow-disturbing are facing to the basal planes tilted.
Preferably, the first flow-disturbing face than the second flow-disturbing face closer to the runner entrance, first side
The angle of the straight flange formed with the two-end-point on the 3rd side is first angle, the described second two ends while with the described 3rd
The angle of the formed straight flange of point is second angle, and the first angle is less than or equal to the second angle.
Preferably, the first angle is 30-60 degree, and the second angle is 45-80 degree；It is highly preferred that described contain
The flow velocity of the liquid flowing of microalgae is 0.5-2.5m/s.
The method of pilot scale culture microalgae of the invention, by increasing capacitance it is possible to increase microalgae motion vertically, makes microalgae in light
Shuttled between area and dark space, so as to improve the efficiency of light energy utilization and photosynthetic efficiency of microalgae, improve the yield of microalgae.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Brief description of the drawings
Fig. 1 is the schematic elevation view of the bioreactor according to one embodiment of the present invention.
Fig. 2 is the schematic top plan view of bioreactor as shown in Figure 1.
Fig. 3 is five kinds of schematic side views of implementation method of bioreactor of the invention.
Fig. 4 is the schematic diagram in the flow-disturbing section of the spoiler of bioreactor of the invention.
Description of reference numerals
1 runner；2 spoilers；21 first flow-disturbing faces；22 second flow-disturbing faces；23 flow-disturbing sections；231 first sides；232 second
Side；233 the 3rd sides；The height of H1 spoilers；The liquid that H2 contains the liquid of microalgae is thick；The two neighboring edge streams streamed between part of L1
The spacing of the length direction in road；The length of L2 runners；The bearing of trend of X runners；Y first directions.
Specific embodiment
Specific embodiment of the invention is described in detail below.It should be appreciated that described herein specific
Implementation method is merely to illustrate and explain the present invention, and is not intended to limit the invention.
The invention provides a kind of method of pilot scale culture microalgae, the method is included under conditions of culture microalgae,
The culture of microalgae is carried out in bioreactor, as depicted in figs. 1 and 2, the reactor includes allowing the liquid flow containing microalgae
Dynamic runner 1 and spoiler 2, spoiler 2 by flow-disturbing section 23 in the first direction Y extend and formed, flow-disturbing section 23 includes
Close-shaped first is sequentially connected with 231, second 232 and the 3rd side 233, first at 231, second 232 and the 3rd
Y extensions form the first flow-disturbing face 21, the second flow-disturbing face 22 and basal plane respectively in the first direction on side 233, and first at 231 and second
232 is straight flange, and basal plane fits in the inner surface setting of runner 1, and the first flow-disturbing face 21 and the second flow-disturbing face 22 are inclined relative to basal plane
Tiltedly set, first direction Y intersects with the bearing of trend X of runner 1.
In the present invention, spoiler 2 by flow-disturbing section 23 in the first direction Y extend and formed, first direction Y can be straight
One or more in line, curve and broken line, but as it is known by the man skilled in the art, spoiler 2 bearing of trend any point
On bearing of trend of the section all with the point it is vertical.
The inner surface setting of runner 1 is fitted in due to the basal plane of spoiler 2, therefore, first direction Y can be according to runner 1
The shape in section and the development length of spoiler 2 determine that the section of runner 1 is the extension for referring to runner 1 perpendicular to runner 1
The section of direction X, when the rectangular cross-section, if spoiler 2 only extends in the bottom surface of runner 1, first direction Y is straight
In line, such as Fig. 3（a）Shown, if spoiler 2 both extends in the bottom surface of runner 1, and side wall in runner 1 extends, then first party
It is broken line to Y, in such as Fig. 3（b）It is shown；When the section is the shape that camber line and straight line are constituted, in such as Fig. 3（c）It is shown, then
One direction Y is curve, is camber line specifically；When the section of the inner surface of runner 1 is the rectangle that base is camber line, such as Fig. 3
In（d）With（e）Shown, if spoiler 2 only extends in the bottom surface of runner 1, first direction is curve, is arc specifically
In line, such as Fig. 3（d）Shown, if spoiler 2 both extends in the bottom surface of runner 1, and side wall in runner 1 extends, then first party
To Y bottom surface be camber line, in side wall be straight line, in such as Fig. 3（e）It is shown.Here for first direction Y is explicitly described, only arrange
Several situations, but not limit are lifted, those skilled in the art should be able to be expressly understood first party according to foregoing description
To Y.
In the present invention, for runner 1 section shape without particular/special requirement, can for those skilled in the art institute it is contemplated that
It is variously-shaped, for example, can be that rectangle, semicircle, circle, base are rectangle etc. of camber line.
In the present invention, the first flow-disturbing face 21 and the second flow-disturbing face 22 are set relative to basal planes tilted, can incline towards basal plane
Tiltedly, it is also possible to dorsad basal planes tilted.Towards basal planes tilted, that is, refer to first at 231 and second 232 respectively with the 3rd side 233
Two-end-point formed straight flange angle be less than 90 degree.Dorsad basal planes tilted, that is, refer to first 232 to distinguish at 231 or second
The angle of the straight flange formed with the two-end-point on the 3rd side 233 is more than 90 degree.In the present invention, the first flow-disturbing face 21 and the second flow-disturbing face
22 are preferably directed towards basal planes tilted.
According to the present invention, although the first flow-disturbing face 21 and the second flow-disturbing face 22 are set relative to basal planes tilted, you can realize
The purpose of the present invention, that is, improve the yield of microalgae, and the first flow-disturbing face 21 and the second flow-disturbing face 22 are preferably directed towards basal planes tilted, can be more
Plus improve the yield of microalgae.But in the case of more preferably, when the flow-disturbing face 22 of the first flow-disturbing face 21 to the second closer to the entrance of runner 1
When, first at 231 and the 3rd angle of the straight flange that 233 two-end-point is formed be first angle, second at 232 and the 3rd
The angle of the straight flange that 233 two-end-point is formed is second angle, and first angle is less than or equal to second angle, can further improve
The yield of microalgae；First angle is 30-60 degree, and second angle is 45-80 degree, can further improve the yield of microalgae；More enter
Under one step preferable case, the flow velocity of the liquid flowing containing microalgae is 0.5-2.5m/s, can more give full play to the " flash of light of microalgae
Effect ", further improves the yield of microalgae.
In the present invention, for first direction Y, if intersect with the bearing of trend X of runner 1, therefore, first direction Y
Except can not it is parallel with the bearing of trend X of runner 1 in addition to, can be with the bearing of trend X of runner 1 into arbitrarily angled, in order that flow-disturbing
Face plays bigger flow-disturbing effect, and under preferable case, first direction Y is vertical with the bearing of trend X of runner 1.
In the present invention, Y extends the inner surface of spoiler 2 length and runner 1 that Y extends in the first direction in the first direction
The ratio of length is preferably 1：1-10, more preferably 1：1-5.Under the preferable case, spoiler 2 can be played more fully
Flow-disturbing effect, can further improve the efficiency of light energy utilization and photosynthetic efficiency of microalgae, further improve the yield of microalgae.
Can be various situations, solution according to first direction Y points for the inner surface length that Y extends in the first direction of runner 1
Release as follows：When in such as Fig. 3（a）When shown, i.e. when first direction Y is straight line, Y extends in the first direction for the inner surface of runner 1
Length be refer to runner 1 bottom surface in the first direction Y extend length, when first direction Y is vertical with the bearing of trend X of runner 1
When, the inner surface length that Y extends in the first direction of runner 1 is the width of the bottom surface for referring to runner 1；When in such as Fig. 3（b）It is shown
When, i.e. when first direction Y is broken line, the inner surface of runner 1 length that Y extends in the first direction be refer to runner 1 bottom surface edge
The length that first direction Y extends adds the side wall length that Y extends in the first direction of runner 1, when first direction Y and runner 1
When bearing of trend X is vertical, the inner surface of runner 1 length that Y extends in the first direction is that the width of the bottom surface for referring to runner 1 is added
Two height of side wall of runner 1；When in such as Fig. 3（c）With（d）When shown, i.e. when first direction Y is curve, the interior table of runner 1
Face in the first direction Y extend length be refer to runner 1 bottom surface in the first direction Y extend length, when first direction Y with stream
When the bearing of trend X in road 1 is vertical, the inner surface length that Y extends in the first direction of runner 1 is the arc of the bottom surface for referring to runner 1
It is long；When in such as Fig. 3（e）When shown, i.e. first direction Y is camber line in bottom surface, when in side, wall is straight line, the inner surface edge of runner 1
The length that first direction Y extends be refer to runner 1 the bottom surface length that Y extends in the first direction plus runner 1 side wall along the
The length that one direction Y extends, when first direction Y is vertical with the bearing of trend X of runner 1, the inner surface of runner 1 is in the first direction
The length that Y extends is refer to runner 1 perpendicular to the base in the section of the bearing of trend X of runner 1 arc length plus two of runner 1
The height of side wall.Here for the inner surface length that Y extends in the first direction for clearly demonstrating runner 1, only list several
Situation, but not limit, those skilled in the art should be able to be expressly understood the inner surface edge of runner 1 according to foregoing description
The length that first direction Y extends.
In Fig. 3（b）-（d）Show the length that Y extends in the first direction of spoiler 2 with the inner surface of runner 1 along first party
The ratio of the length extended to Y is 1：1 situation；In Fig. 3（a）With（e）Show the length that Y extends in the first direction of spoiler 2
The situation of the ratio less than 1 of degree and the inner surface of runner 1 length that Y extends in the first direction, i.e. spoiler 2 Y in the first direction
The length of extension be only runner 1 inner surface in the first direction Y extend length a part.
In the present invention, for spoiler 2 number without particular/special requirement, in order to play more preferable flow-disturbing effect, spoiler 2 is excellent
Elect multiple as, i.e., it is more than one.The shape of multiple spoilers 2 can be the same or different.When spoiler 2 is for multiple, phase
The ratio of the spacing L1 and the length L2 of runner 1 of the length direction along runner 1 between adjacent two spoilers 2 is preferably≤0.5.Phase
The spacing L1 of the length direction along runner 1 between adjacent two spoilers 2 is as shown in Figure 1.Under the preferable case, can be further
The efficiency of light energy utilization and photosynthetic efficiency of microalgae are improved, the yield of microalgae is further improved.
In the present invention, the ratio of the height H1 of the spoiler 2 and liquid thickness H2 of the liquid containing microalgae is preferably 1：2-20, enters
One step is preferably 1：5-15.Under the preferable case, spoiler 2 can play more fully flow-disturbing effect.As it was previously stated, flow-disturbing
It refers to the peak of spoiler 2 to the vertical range of the basal plane of spoiler 2 that the height H1 of part 2 is.The height H1 of spoiler 2 with contain
There is the liquid thickness H2 of the liquid of microalgae as shown in Figure 1.
In the present invention, the basal plane of spoiler 2 preferably fits in the not smooth surface setting of runner 1.Under the preferable case, can
The efficiency of light energy utilization and photosynthetic efficiency of microalgae are further improved, the yield of microalgae is further improved.This is because smooth surface is not attached
Near microalgae can't accept illumination, and the basal plane of spoiler 2 fits in the not smooth surface setting of runner 1, can make to can't accept illumination
Not smooth surface near microalgae spoiler 2 flow-disturbing effect under move at illumination, so as to improve microalgae in runner 1
The efficiency of light energy utilization and photosynthetic efficiency, improve the yield of microalgae.
In the present invention, the liquid thickness H2 of the liquid containing microalgae, can be thick using the conventional liquid in this area without particular/special requirement, example
Such as, the liquid thickness H2 of the liquid containing microalgae can be 1-30cm.
It will be understood by those skilled in the art that microalgae is flowed in runner in the presence of pump, therefore, in order to
The energy consumption of pump is reduced, the inclination angle of runner 1 is preferably 0-3 degree.The inclination angle of runner 1 refers to runner 1 with respect to the horizontal plane
Angle of inclination.
It can also be many that the runner 1 of the bioreactor of the pilot scale culture microalgae that the present invention is provided can be one
It is individual, can be according to the amount setting of actual culture microalgae.
In the present invention, for runner 1 wall thickness without particular/special requirement, can be using the conventional setting in this area.For runner 1
Can be one or more in transparent glass, plastics and resin with the material of spoiler 2 also without particular/special requirement, it is also possible to
It it is one or more in opaque stainless steel, aluminium alloy and cement, the material of spoiler 2 and the material of runner 1 can phases
With can also be different, in order to easily manufactured, preferably the material of spoiler 2 be identical with the material of runner 1.
In the present invention, spoiler 2 can also be able to be hollow structure for solid construction.
In the present invention, for spoiler 2 the inner surface of runner 1 set-up mode also without particular/special requirement, can using this
The thinkable various modes of art personnel institute, for example, spoiler 2 and runner 1 can be formed integrally as, it is also possible to
Spoiler 2 is adhered to or is assembled on runner 1.
The bioreactor of pilot scale culture microalgae of the invention can be Race-way photobioreactor, in such as Fig. 3
（c）It is shown；Can also be closed photo bioreactor, in such as Fig. 3（a）、（b）、（d）With（e）It is shown.
The inventive method is, by setting spoiler in bioreactor, to be carried by the cooperation of spoiler and flow velocity
The yield of microalgae high, therefore, for cultivating the condition of microalgae without particular/special requirement, condition commonly used in the art can be used, for example,
The condition for cultivating microalgae can include：Temperature is 15-40 DEG C, preferably 25-35 DEG C；Light intensity is 2000-30000 luxs, excellent
Elect 5000-20000 luxs as；It is passed through the mixed gas of air and carbon dioxide；Control the pH value of the nutrient solution containing microalgae
It is 6-9.
In the present invention, the species for microalgae has no particular limits, preferably oil-producing microalgae, more preferably with larger
Industrial utilization oil-producing engineering microalgae, for example, microalgae can be selected from chlorella, grid algae, spirulina, chrysophyceae and phaeodactylum tricornutum
Refer at least one in algae.
Embodiment
The present invention is further illustrated for following embodiment, but and is not so limited the present invention.
In the following Examples and Comparative Examples：
Chlorella is purchased from Inst. of Hydrobiology, Chinese Academy of Sciences, and nutrient solution is BG11.
Microalgae is cultivated in bioreactor, using following condition：It is first anti-to photo-biological with sodium hypochlorite before inoculation
Device is answered to carry out disinfection, then with sterile water wash, interval is inoculated with after 12 hours.Nutrient solution is BG11, in terms of the dry weight of chlorella,
Concentration when chlorella is inoculated with is 0.13mg/l, controls algae solution temperature for 25 DEG C, is passed through the mixed gas of air and carbon dioxide,
The pH value of the nutrient solution containing microalgae is controlled for 6-9, light intensity is 12000 luxs.Periodically supplemented in nutrient solution during culture
Nutritive salt, and adding a small amount of water makes culture algae solution volume keep constant.Each embodiment and the condition of comparative example culture chlorella
It is identical.
Embodiment 1
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
Chlorella is cultivated under natural lighting.Bioreactor is by upper and lower two runners 1, liquid reserve tank and circulation pump group
Into runner 1 is effective plane of illumination region, each runner 1 150cm long, 10cm wide, liquid thickness 5cm, without inclination angle, the bottom surface of runner 1
It is not smooth surface, is provided with 10 spoilers 2 on the not smooth surface of each runner 1, spoiler 2 is by flow-disturbing section 23 along the
One direction Y extends and is formed, and flow-disturbing section 23 is triangle, including first at 231, second 232 and the 3rd side 233, and first
At 231, second 232 and the 3rd side 233 in the first direction Y extend form the first flow-disturbing face 21, the and of the second flow-disturbing face 22 respectively
Basal plane, basal plane fits in the inner surface setting of runner 1, the flow-disturbing face 22 of the first flow-disturbing face 21 to the second closer to runner 1 entrance,
First at 231 and the 3rd 233 angle be 30 degree, second at 232 and the 3rd 233 angle be 45 degree, first direction Y with
The bearing of trend X of runner 1 is vertical, and Y prolongs the inner surface of the length that Y extends in the first direction of spoiler 2 and runner 1 in the first direction
The ratio of the length stretched is 1：1.The spacing L1 of the length direction along runner 1 between two neighboring spoiler 2 and the length of runner 1
The ratio for spending L2 is 1：10.The ratio of the height H1 of the spoiler 2 and liquid thickness H2 of the liquid containing chlorella is 1：5.Liquid reserve tank
During the interior liquid containing chlorella flows into upper runner in the presence of circulating pump, flowed into the presence of initial velocity and flowed down
Road, flows back to liquid reserve tank, and Cyclic culture controls flow velocity of the liquid containing chlorella in runner for 0.5m/s by circulating pump.
In terms of the dry weight of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Embodiment 2
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
Chlorella is cultivated under natural lighting.Bioreactor is by upper and lower two runners 1, liquid reserve tank and circulation pump group
Into, runner 1 is effective plane of illumination region, each runner 1 150cm long, 10cm wide, liquid thickness 15cm, and runner inclination angle is 1 degree,
The bottom surface of runner 1 is not smooth surface, and 10 spoilers 2 are provided with the not smooth surface of each runner 1, and spoiler 2 passes through flow-disturbing
Y extends and is formed in the first direction in section 23, and flow-disturbing section 23 is triangle, including first at 231, second 232 and the 3rd
Side 233, first at 231, second 232 and the 3rd side 233 in the first direction Y extend form the first flow-disturbing face 21, second respectively
Flow-disturbing face 22 and basal plane, basal plane fit in the inner surface setting of runner 1, and the flow-disturbing face 22 of the first flow-disturbing face 21 to the second is closer to stream
The entrance in road 1, first at 231 and the 3rd 233 angle be 45 degree, second at 232 and the 3rd 233 angle be 75 degree,
First direction Y is vertical with the bearing of trend X of runner 1, the inner surface edge of the length that Y extends in the first direction of spoiler 2 and runner 1
The ratio of the length that first direction Y extends is 1：3.The spacing L1 of the length direction along runner 1 between two neighboring spoiler 2
It is 1 with the ratio of the length L2 of runner 1：15.The height H1 of spoiler 2 is with the ratio of the liquid thickness H2 of the liquid containing chlorella
1：10.During the liquid containing chlorella in liquid reserve tank flows into upper runner in the presence of circulating pump, in the effect of initial velocity
Lower inflow lower flow channel, flows back to liquid reserve tank, and Cyclic culture controls flow velocity of the liquid containing chlorella in runner by circulating pump
It is 1.5m/s.In terms of the dry weight of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Embodiment 3
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
Chlorella is cultivated under natural lighting.Bioreactor is by upper and lower two runners 1, liquid reserve tank and circulation pump group
Into, runner 1 is effective plane of illumination region, each runner 1 150cm long, 10cm wide, liquid thickness 30cm, and runner inclination angle is 3 degree,
The bottom surface of runner 1 is not smooth surface, and 10 spoilers 2 are provided with the not smooth surface of each runner 1, and spoiler 2 passes through flow-disturbing
Y extends and is formed in the first direction in section 23, and flow-disturbing section 23 is triangle, including first at 231, second 232 and the 3rd
Side 233, first at 231, second 232 and the 3rd side 233 in the first direction Y extend form the first flow-disturbing face 21, second respectively
Flow-disturbing face 22 and basal plane, basal plane fit in the inner surface setting of runner 1, and the flow-disturbing face 22 of the first flow-disturbing face 21 to the second is closer to stream
The entrance in road 1, first at 231 and the 3rd 233 angle be 60 degree, second at 232 and the 3rd 233 angle be 80 degree,
First direction Y is vertical with the bearing of trend X of runner 1, the inner surface edge of the length that Y extends in the first direction of spoiler 2 and runner 1
The ratio of the length that first direction Y extends is 1：5.The spacing L1 of the length direction along runner 1 between two neighboring spoiler 2
It is 1 with the ratio of the length L2 of runner 1：20.The height H1 of spoiler 2 is with the ratio of the liquid thickness H2 of the liquid containing chlorella
1：15.During the liquid containing chlorella in liquid reserve tank flows into upper runner in the presence of circulating pump, in the effect of initial velocity
Lower inflow lower flow channel, flows back to liquid reserve tank, and Cyclic culture controls flow velocity of the liquid containing chlorella in runner by circulating pump
It is 2.5m/s.In terms of the dry weight of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Embodiment 4
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
According to the method culture chlorella of embodiment 1, unlike, first at 231 and the 3rd 233 angle be 20
Degree, second at 232 and the 3rd 233 angle be 85 degree.In terms of the dry weight of chlorella, the concentration of different incubation time chlorellas
As shown in table 1.
Embodiment 5
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
According to the method culture chlorella of embodiment 1, unlike, first at 231 and the 3rd 233 angle be 80
Degree, second at 232 and the 3rd 233 angle be 30 degree.In terms of the dry weight of chlorella, the concentration of different incubation time chlorellas
As shown in table 1.
Embodiment 6
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
According to the method culture chlorella of embodiment 1, unlike, control the liquid containing chlorella to exist by circulating pump
Flow velocity in runner is 3m/s.In terms of the dry weight of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Embodiment 7
The present embodiment is used for the method for illustrating pilot scale culture microalgae of the invention.
According to the method culture chlorella of embodiment 1, unlike, control the liquid containing chlorella to exist by circulating pump
Flow velocity in runner is 0.2m/s.In terms of the dry weight of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Comparative example 1
According to the method culture chlorella of embodiment 1, unlike, flow-disturbing section 23 is rectangle, is affixed on not smooth surface
While in embodiment 1 the 3rd while it is 233 isometric, rectangular another length of side is equal with the H1 high of the intermediate cam shape of embodiment 1.With
The dry weight meter of chlorella, the concentration of different incubation time chlorellas is as shown in table 1.
Comparative example 2
According to the method culture chlorella of embodiment 1, unlike, spoiler is not added with bioreactor.With small
The dry weight meter of ball algae, the concentration of different incubation time chlorellas is as shown in table 1.
Table 1
Embodiment 1-7 is compared as can be seen that pilot scale culture of the invention with comparative example 1 and comparative example 2 respectively
The method of microalgae can improve the yield of microalgae.
Embodiment 1 is compared with embodiment 4 and embodiment 5 respectively as can be seen that first angle is less than or equal to second
Angle, is more beneficial for the raising of the yield of microalgae；First angle is 30-60 degree, and second angle is 45-80 degree, is further had
Beneficial to the raising of the yield of microalgae.Embodiment 1 is compared with embodiment 6 and embodiment 7 as can be seen that containing microalgae respectively
Liquid flowing flow velocity be 0.5-2.5m/s, be more beneficial for the raising of the yield of microalgae.
The method of pilot scale culture microalgae of the invention, by increasing capacitance it is possible to increase microalgae motion vertically, makes microalgae in light
Shuttled between area and dark space, so as to improve the efficiency of light energy utilization and photosynthetic efficiency of microalgae, improve the yield of microalgae.
The preferred embodiment of the present invention is described in detail above in association with accompanying drawing, but, the present invention is not limited to above-mentioned reality
The detail in mode is applied, in range of the technology design of the invention, various letters can be carried out to technical scheme
Monotropic type, these simple variants belong to protection scope of the present invention.
It is further to note that each particular technique feature described in above-mentioned specific embodiment, in not lance
In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the present invention to it is various can
The combination of energy is no longer separately illustrated.
Additionally, can also be combined between a variety of implementation methods of the invention, as long as it is without prejudice to originally
The thought of invention, it should equally be considered as content disclosed in this invention.
Claims (10)
1. a kind of method of pilot scale culture microalgae, methods described is included under conditions of culture microalgae, in bioreactor
In carry out the culture of microalgae, it is characterised in that the reactor includes the runner (1) for allowing the liquid containing microalgae to flow and disturbs
Stream part (2), the spoiler (2) by flow-disturbing section (23) in the first direction (Y) extend and formed, the flow-disturbing section (23)
Including being sequentially connected with into close-shaped first (232) and the 3rd side (233) in (231), second, first side (231),
Described second at (232) and the described 3rd (233) along the first direction (Y) extend formed respectively the first flow-disturbing face (21),
Second flow-disturbing face (22) and basal plane, described first at (231) and described second (232) be straight flange, the basal plane fits in institute
The inner surface setting of runner (1) is stated, the first flow-disturbing face (21) and the second flow-disturbing face (22) are inclined relative to the basal plane
Tiltedly set, the first direction (Y) is vertical with the bearing of trend (X) of the runner (1)；
Wherein, the first flow-disturbing face (21) and the second flow-disturbing face (22) are towards the basal planes tilted；
Wherein, the first flow-disturbing face (21) than the second flow-disturbing face (22) closer to the runner (1) entrance, described
The angle of the straight flange that (231) are formed with the two-end-point of the 3rd side (233) is first angle on one side, second side
(232) angle of the straight flange formed with the two-end-point of the 3rd side (233) is second angle, and the first angle is less than etc.
In the second angle.
2. method according to claim 1, wherein, the first angle is 30-60 degree, and the second angle is 45-80
Degree.
3. method according to claim 2, wherein, the flow velocity of the liquid flowing containing microalgae is 0.5-2.5m/s.
4. the method according to any one in claim 1-3, wherein, the spoiler (2) is along the first direction (Y)
The ratio of the length that the length of extension extends with the inner surface of the runner (1) along the first direction (Y) is 1：1-10.
5. the method according to any one in claim 1-3, wherein, the spoiler (2) is multiple, two neighboring institute
State the spacing (L1) of the length direction along the runner (1) between spoiler (2) and the ratio of the length (L2) of the runner (1)
Value≤0.5.
6. the method according to any one in claim 1-3, wherein, the height (H1) of the spoiler (2) with it is described
The ratio of liquid thickness (H2) of the liquid containing microalgae is 1：2-20.
7. the method according to any one in claim 1-3, wherein, the basal plane fits in the runner (1) no
Smooth surface is set.
8. the method according to any one in claim 1-3, wherein, liquid thickness (H2) of the liquid containing microalgae is
1-30cm。
9. the method according to any one in claim 1-3, wherein, the inclination angle of the runner (1) is 0-3 degree.
10. the method according to any one in claim 1-3, wherein, the microalgae is selected from chlorella, grid algae, spiral
At least one in algae, chrysophyceae and Phaeodactylum tricornutum.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101899385A (en) * | 2009-05-27 | 2010-12-01 | 中国科学院过程工程研究所 | Closed photo bioreactor for enhancing light energy utilization rate of microalgae mass culture |
| CN102260629A (en) * | 2010-05-28 | 2011-11-30 | 中国科学院过程工程研究所 | Plate-type photobioreactor |
| CN102787066A (en) * | 2011-05-20 | 2012-11-21 | 曲作鹏 | Enclosed perfusion-type photobioreactor |
-
2013
- 2013-07-19 CN CN201310305853.1A patent/CN104293673B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101899385A (en) * | 2009-05-27 | 2010-12-01 | 中国科学院过程工程研究所 | Closed photo bioreactor for enhancing light energy utilization rate of microalgae mass culture |
| CN102260629A (en) * | 2010-05-28 | 2011-11-30 | 中国科学院过程工程研究所 | Plate-type photobioreactor |
| CN102787066A (en) * | 2011-05-20 | 2012-11-21 | 曲作鹏 | Enclosed perfusion-type photobioreactor |
Non-Patent Citations (1)
| Title |
|---|
| 平板式光生物反应器的Parietochloris incisa超高密度培养;王长海等;《烟台大学学报(自然科学与工程版)》;20000715(第03期);230-234 * |
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