CN106906123A - The culture apparatus of filamentous algae - Google Patents

The culture apparatus of filamentous algae Download PDF

Info

Publication number
CN106906123A
CN106906123A CN201710232865.4A CN201710232865A CN106906123A CN 106906123 A CN106906123 A CN 106906123A CN 201710232865 A CN201710232865 A CN 201710232865A CN 106906123 A CN106906123 A CN 106906123A
Authority
CN
China
Prior art keywords
nutrient solution
filamentous algae
lamina affixad
culture
pond
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710232865.4A
Other languages
Chinese (zh)
Inventor
胡强
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sdic Biotechnology Investment Co ltd
Original Assignee
STATE DEVELOPMENT & INVESTMENT Corp (SDIC)
Chinese Electronics Engineering Design Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by STATE DEVELOPMENT & INVESTMENT Corp (SDIC), Chinese Electronics Engineering Design Institute filed Critical STATE DEVELOPMENT & INVESTMENT Corp (SDIC)
Priority to CN201710232865.4A priority Critical patent/CN106906123A/en
Publication of CN106906123A publication Critical patent/CN106906123A/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06Plates; Walls; Drawers; Multilayer plates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Cultivation Of Seaweed (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention discloses a kind of culture apparatus of filamentous algae, the culture apparatus includes a filamentous algae culture pond, at least one lamina affixad is submerged in the culture pond, at least one water stream channel is formed in the culture pond, nutrient solution in the water stream channel flows towards a predetermined direction, the lamina affixad is located in the water stream channel of the nutrient solution, and the filamentous algae in the nutrient solution is constantly enriched on the lamina affixad with the flowing of nutrient solution.So that the filamentous algae in algae solution is unlikely to excessive concentration and deposits to culture pond bottom, illuminating area is improved, it is to avoid excessive shearing force is destroyed to the structure of filamentous algae, moreover it is possible to save energy consumption, the conversion of the culture environment being also convenient in filamentous algae incubation.

Description

The culture apparatus of filamentous algae
Technical field
This invention relates generally to a kind of culture apparatus of microalgae, in particular to a kind of culture dress of filamentous algae Put.
Background technology
Microalgae is a class widely distributed in land, ocean, the nutritious, autophyte that photosynthetic availability is high, cell generation Polysaccharide, protein, pigment of generation etc. are thanked, it is had in fields such as food, medicine, genetic engineering, liquid fuels good DEVELOPMENT PROSPECT.
Filamentous algae belongs to one kind of microalgae, mainly there is the thread Trentepohlia in Chlorophyta, Cyanophyta, Bacillariophyta, Xanthophyta, its Most of algal filament by hundreds of to thousands of cells formed it is single-row or tool branch form, its group size can reach millimeter or li Meter level.The characteristics of filamentous algae has volume larger compared to unicellular algas such as grid algae, chlorellas.Just because of filamentous algae volume It is larger, therefore it is especially susceptible to results, can reduce results cost;Further, since the size of filamentous algae is much larger than wheel animalcule, its is unfavorable In protozoa animal such as wheel animalcule or ciliophoran phagocytosis, there is very big advantage in terms of to disease and insect resistance.These features are all Not available for the small size algae such as chlorella, grid algae.Therefore in recent years, research of the expert in some microalgae fields to filamentous algae As focus.For example in Liu a Chinese invention patent CN103960117B in day et al. just once mentioned what is mentioned from Huang Sizao Bio oil, it contains eicosapentaenoic acid EPA, Cytochrome P450 Arachidonic Acid Epoxygenase and other long-chain polyunsaturated fatty acids PUEA.These Compound has an application in food and medicine field, EPA have regulation blood fat, anticoagulation and antithrombus formation, expansion of blood vessels and The effect for reducing blood pressure;There is AA the physiology such as the function of reducing blood viscosity, enhancing blood vessel elasticity and esterification body's cholesterol to live Property, there is certain effect to cardiovascular and diabetes and tumour.
But the culture of filamentous algae can also run into some problems.During culture, if in the case that mixing intensity is relatively low, algae solution In algae can deposit quickly, causing the algae for being deposited on the bottom to receive illumination cannot grow and occur rotten or rotten It is rotten, and culture pond bottom limited area, cause overall light utilization ratio relatively low;And if mixing intensity is excessive, filamentous algae is again Easily influenceed by shearing force and produced fracture of wire phenomenon, be destroyed into cell wall structure, when fracture of wire reaches to a certain degree, algae is thin Born of the same parents' vigor is badly damaged, and is equally unfavorable for the raising of yield.In addition, the culture of Huang Sizao generally comprises two stages, i.e., it is biological Matter rapid increase stage and oil and fat accumulation stage, the nutrient media components in the two stages often have a larger difference, therefore The increase of yellow silk algal biomass to a certain extent when, it is necessary to be moved into nutrition limitation media environment in, coerced or lured Lead oil and fat accumulation., it is necessary to filamentous algae is collected in new media environment is moved to, move integrally to a new training In foster base.
Half dry solid-state adherent method culture filamentous algae can be used although referring in the prior art, but actual conditions are, due to Filamentous algae is that hundreds of to thousands of cells form single-row, and demand during growth to water and culture medium is larger, is only dripped by top The culture medium for leaking down cannot well meet growth needs, and because environment evaporation capacity is very big, be cultivated on a small scale through laboratory It was found that, half dry solid-state adhere-wall culture effect is poor, and biomass gather way and are difficult to improve again.If increasing the instillation of nutrient solution Speed, the algae adhered on solid state rheology plate can be washed away, it is impossible to be attached to culture plate surface again, this shortcoming is always again The defect of half dry solid-state adhere-wall culture.
In summary, existing filamentous algae cultural method and culture apparatus Shortcomings, it is impossible to adapt to for filamentous algae Culture.
Above- mentioned information is only used for strengthening the understanding to background of the invention, therefore it disclosed in the background section Can include not constituting the information to prior art known to persons of ordinary skill in the art.
The content of the invention
A primary object of the present invention is at least one defect for overcoming above-mentioned prior art, there is provided one kind is suitable to training The culture apparatus of filamentous algae is supported, culture area can be increased in the case where mixing intensity is reduced, improved light utilization ratio and subtract Few energy consumption, the fracture of wire phenomenon that the mechanical force that reduction homogenizes is caused to filamentous algae, while being also convenient for changing the culture medium of filamentous algae Environment.
For achieving the above object, the present invention is adopted the following technical scheme that:
According to an aspect of the invention, there is provided a kind of culture apparatus of filamentous algae, the culture apparatus is including one Filamentous algae culture pond, at least one current of formation at least one lamina affixad, the culture pond is submerged in the culture pond and is led to Road, the nutrient solution in the water stream channel flows towards a predetermined direction, and the lamina affixad is located at the water stream channel of the nutrient solution Interior, filamentous algae in the nutrient solution is constantly enriched on the lamina affixad with the flowing of nutrient solution.
Wherein, the flowing velocity of nutrient solution is 0.1m/s~0.5m/s, the thickness of the nutrient solution in culture pond for 10cm~ 50cm, relative to general raceway pond reactor, nutrient solution is in relatively low flowing velocity.
In a feasible embodiment of the invention, wherein the culture pond is raceway pond, one is provided with the middle of the runway Individual demarcation strip, the water stream channel is formed at the dividing plate surrounding;Or the culture pond is circular pond, is provided with circular pond One demarcation strip, the water stream channel is formed at the dividing plate surrounding.
In a feasible embodiment of the invention, wherein the nutrient solution in the culture pond drives a stirring through motor Oar drives or is driven through gas explosion, the nutrient solution in culture pond is flowed towards a predetermined direction.
In a feasible embodiment of the invention, wherein the lamina affixad soaks perpendicular to the direction of the nutrient solution thickness Not in nutrient solution, the length direction of the lamina affixad is parallel with the flow direction of the nutrient solution, and water stream channel is partitioned into Multiple weep passages.
In a feasible embodiment of the invention, wherein the lamina affixad soaks perpendicular to the direction of the nutrient solution thickness Not in nutrient solution, the length direction of the lamina affixad is at an angle with the flow direction of the nutrient solution, and the angle is more than 0 ° is less than or equal to 90 °.
In a feasible embodiment of the invention, the lamina affixad is latticed plate, thereon with several mesh, The mesh can flow through for nutrient solution.
Wherein, the specification of the mesh is 1mm2~25mm2
In a feasible embodiment of the invention, the surface that the lamina affixad is contacted with nutrient solution has some protruding from The burr or crotch on the surface, are similar to the hook side of VELCRO.
Wherein, the length that the burr or crotch protrude from the surface is 0.5mm~5mm.
In a feasible embodiment of the invention, the top view of the lamina affixad is in turning type, wave linear shape, jaggies Shape is concavo-convex linear.
In a feasible embodiment of the invention, the top view of the lamina affixad is in " rich " font or fishbone.
In a feasible embodiment of the invention, the lamina affixad is to fix one by the surface of layer of metal waffle slab The hook side of VELCRO and constitute.
As shown from the above technical solution, the advantage and good effect of the culture apparatus of filamentous algae of the invention are:
The culture apparatus of filamentous algae of the present invention, it is thread in flow process because nutrient solution flows towards a certain predetermined direction Algae can be attached to the surface of lamina affixad, so that the filamentous algae in algae solution is unlikely to excessive concentration and deposits to culture pond bottom (while nutrient solution can still allow light to enter into below liquid level), and the filamentous algae on lamina affixad still can be contacted constantly Nutrition composition in culture medium, and the continued growth under illumination condition;Therefore the culture area and light of former culture pond are improve Show up product, particularly when lamina affixad is extended along current flow direction, water stream channel is partitioned into some weep passages In the case of, culture/illuminating area will be in a times increase.It is thread due to what is dissociated in nutrient solution in culture apparatus of the invention Concentration of algae is relatively low, therefore water velocity can slow down with respect to raceway pond etc., it is also possible to avoid the machinery of excessive mixing intensity Shearing force is destructive to the structure of filamentous algae, moreover it is possible to save energy consumption.
As incubation time increases, substantial amounts of filamentous algae can be saved bit by bit on lamina affixad, can be taken out from nutrient solution easily These lamina affixads, are then transferred into carrying out inducing/coercing cultivation in new media environment, are transferred to the culture pond of new culture medium When middle, only need to gently swing or changes and water (flow) direction angle the filamentous algae on lamina affixad can be just discharged into it is new In nutrient solution, and the filamentous algae being free in algae solution in former culture pond is alternatively arranged as algae kind continued growth.Additionally, this method is also Can be used for feeding fish, the lamina affixad that will be covered with filamentous algae is placed into fish nursing area, the algae for allowing fish to peck on lamina affixad Body.
Brief description of the drawings
By being considered in conjunction with the accompanying following the following detailed description of the embodiment of the present invention, various targets of the invention, Feature and advantage will become apparent.Accompanying drawing is only exemplary diagram of the invention, is not necessarily drawn to scale. In the accompanying drawings, same reference represents same or similar part all the time.Wherein:
Fig. 1 is the overlooking the structure diagram of the culture apparatus of the filamentous algae of the embodiment of the present invention one.
Fig. 2 is the overlooking the structure diagram of the culture apparatus of the filamentous algae of the embodiment of the present invention two.
Fig. 3 is the overlooking the structure diagram of the culture apparatus of the filamentous algae of the embodiment of the present invention three.
Fig. 4 is the overlooking the structure diagram of the culture apparatus of the filamentous algae of the embodiment of the present invention four.
Fig. 5 A-5C are the structural representation of the lamina affixad of the embodiment of the present invention.
Fig. 6 A, Fig. 6 B~Fig. 6 G are the top view schematic diagram of the lamina affixad of the embodiment of the present invention.
Fig. 7 is the lamina affixad made using the hook side of VELCRO, its experiment effect photo.
Specific embodiment
Example embodiment is described more fully with referring now to accompanying drawing.However, example embodiment can be with various shapes Formula is implemented, and is not understood as limited to implementation method set forth herein;Conversely, thesing embodiments are provided so that the present invention will Fully and completely, and by the design of example embodiment those skilled in the art is comprehensively conveyed to.Identical accompanying drawing in figure Mark represents same or similar structure, thus will omit their detailed description.
Embodiment one
It is the culture apparatus schematic diagram of the filamentous algae of embodiment one referring to Fig. 1, culture apparatus mainly includes raceway pond 1, stirring Impeller 3 and lamina affixad 4.In raceway pond 1 be equipped with micro algae culturing liquid 2, medium depth can example be 10cm~50cm, wherein connecing Planting has filamentous algae.Raceway pond 1 includes pool wall 11 and the demarcation strip 12 in the middle of raceway pond 1, and demarcation strip 12 makes shape in raceway pond 1 Into one or more water stream channels P.In water stream channel P, the rotation of the agitator arm 3 can drive water to one agitator arm 3 Nutrient solution 2 in circulation road P flows (as shown by arrows in FIG.) towards a predetermined direction, and flowing velocity is 0.1m/s~0.5m/s. Wherein, may be selected to be provided with several lamina affixads 4 in water stream channel P, these lamina affixads 4 are perpendicular to the height of the nutrient solution 2 Direction is immersed in nutrient solution 2, and the length direction of lamina affixad 4 is parallel with the flow direction of the nutrient solution, by water stream channel P It is partitioned into some weep passages 41.Nutrient solution 2 flows through these weep passages 41, because filamentous algae length can reach milli Rice or Centimeter Level, and be readily adhered in the both side surface of lamina affixad 4.The mixing speed of control agitator arm 3 may be selected, makes it Shearing force is unlikely to structure that is excessive and destroying filamentous algae, causes the cavitation of the frustule of filamentous algae, while will will not also be enriched with The nutrient solution 2 all flowed in the filamentous algae of the both side surface of lamina affixad 4 is taken away.
Wherein, it is the ability for adhering to filamentous algae of increase lamina affixad 4, the implementation method of lamina affixad 4 can be exemplified by Fig. 5 A Form, i.e. lamina affixad 4 has some burrs for protruding from the surface or crotch 44 with the both side surface that nutrient solution 2 is contacted, this The length that a little burrs or crotch 44 protrude from surface can be 0.5mm~5mm, so that more effectively by the filamentous algae in nutrient solution 2 Hooking is enriched in surface.These burrs and crotch 44 may be selected to be nylon, plastics or wire and be made.
As cultivated days increase, increasing filamentous algae can be accumulated in the both side surface of lamina affixad 4, and nutrient solution 2 In concentration can't increase too much, therefore in this period, natural light can be still irradiated to below liquid level, made in nutrient solution 2 Algae receive and use sunlight, and the algae accumulated on the two sides of lamina affixad 4 can also receive sunlight, while using nutrient solution Nutritional ingredient continued growth in 2.After the filamentous algae on lamina affixad 4 builds up to certain thickness, but through monitoring, algae is simultaneously not up to Need to switch over the growth phase of culture medium, now may be selected to increase the mixing speed of agitator arm 3 suddenly, improve current and follow Ring speed, the filamentous algae adhered on the side of lamina affixad 4 is washed away and is immersed into nutrient solution 2, then carry out the culture of a period of time;Or Person swings back and forth lamina affixad 4 in algae solution, the algae of its surface attachment is partly into nutrient solution 2.
When filamentous algae culture to logarithmic phase, (general culture 6-9 days) by filamentous algae, it is necessary to be transferred to new culture basic ring (such as low nitrogen or few nitrogen environment) carries out induction oil-producing in border, now, it is only necessary to be submerged in lamina affixad 4 in raceway pond 1 distinguish ( In some embodiments, the module of polylith lamina affixad 4 can be melted into an entirety) gently taken out from original fluid 2, it is placed into new training In nutrient solution.Into in new nutrient solution, it is only necessary to swing or shake lamina affixad 4, it is possible to be discharged into the filamentous algae on lamina affixad 4 Fiber differentiation is carried out in new nutrient solution environment.Such as similar method, it is also possible to the filamentous algae on lamina affixad 4 is placed into fish and is raised Area is supported, is pecked at for fish.And a small amount of filamentous algae remaining in original fluid 2, can be supplemented with after a certain amount of nutrient solution as next The algae kind criticized continues to cultivate.
Wherein, because filamentous algae is constantly bred, but part filamentous algae can be enriched to the surface of lamina affixad 4, therefore nutrient solution 2 In concentration of algae will not be too dense, be still available for light to enter below liquid level, agitator arm 3 can reduce stirring without too fast The mechanical shear stress of impeller 3 brings infringement to algae, while filamentous algae can't be made largely to deposit to the bottom of raceway pond 1.Additionally, Light irradiation area is greatly improved, optical energy utilization efficiency is improved.
For convenience of description, if one length of raceway pond of specific example is 1m, width is 0.5m, and medium depth is 0.2m, then when algae solution concentration is higher, the area that sunshine can irradiate is 1m*0.5m=0.5 ㎡.Carried according to the embodiment of the present invention The culture apparatus of confession, may be selected to place 2 pieces of lamina affixads 4 in the raceway pond, and the thickness of lamina affixad 4 is negligible, by original 0.25m Water stream channel P wide separates 3 weep passages 41, then the both side surface product ≈ 0.2m*1m*2*4=1.6 of each lamina affixad 4 ㎡, the illumination gross area=0.5 ㎡+1.6 ㎡=2.1 ㎡, close to more than 4 times of original light-receiving area.It can be seen that, according to training of the invention Device is supported, light-receiving area can be increased really, particularly in the cultivating system of natural light irradiation.
Wherein, in the present embodiment agitator arm 3 more alternatively into one group of aeration tube.By to being passed through CO in nutrient solution 22Gas Body, CO2It is the photosynthetic necessary nutrition of microalgae, CO is being passed through to nutrient solution 22While, also provide whereby and promote nutrient solution 2 Towards the driving force of predetermined direction flowing.
Embodiment two
It is the structural representation of embodiments of the invention two referring to Fig. 2.The present embodiment mainly exists with the difference of embodiment one The driving force of form, the nutrient solution flowing in culture pond.In the present embodiment, culture pond can be a circular pond 10, circular pond 10 Demarcation strip 120 including pool wall 110 and in the middle of pond, demarcation strip 120 makes to form a water stream channel P in pond.Drive culture Power resources of the liquid 2 towards predetermined direction flowing in water stream channel P are one group of CO2Gas explosion mouth 6.One aeration tube 5 connects CO2Gas Source, aeration tube 5 includes two manifolds 51,52 positioned at (i.e. relative to bottom of pond different height) at liquid level different depth.In manifold 51st, a row of openings towards unidirectional gas explosion mouth 6 is respectively equipped with 52.One group of lamina affixad 4 is provided with water stream channel P, this It with the center in circular pond 10 is the different circular arc of center of circle radius size that the top view of a little lamina affixads 4 is in, each two lamina affixad 4 it Between form a spacing so that water stream channel p-shaped is into multiple narrow sub- water stream channels 41.The top of multiple lamina affixads 4 is with more Individual connecting rod 42 links together, and multiple lamina affixads 4 is formed a module for entirety, is easy to be carried out by way of lifting Install and transfer culture pond.
Embodiment three
It is the structural representation of embodiments of the invention three referring to Fig. 3.The present embodiment mainly exists with the difference of embodiment one In, wherein the length direction of lamina affixad 4 is no longer parallel with the water (flow) direction in water stream channel P, but perpendicular relation.Wherein, The preferably selection of lamina affixad 4 is grid shaped board chip architecture, and the structure of example, is provided with several mesh on lamina affixad 4 as shown in Figure 5 B 43, the aperture of these mesh 43 may be selected to be 1mm2~25mm2, such as lamina affixad 4 is made up of plastics or metal, can be integrated into Type or knitting forming.Likewise, in the present embodiment, multiple lamina affixads 4 are assembled into a module with certain spacing, preferably respectively Lamina affixad 4 has the mesh 43 of different pore size size respectively, makes the aperture of mesh 43 being located on water stream channel P upstreams lamina affixad 4 Maximum, the aperture of the mesh 43 of the lamina affixad 4 of most downstream is minimum.Found by experiment, if mesh 43 on upstream lamina affixad 4 Aperture is too small, and current flow resistance will be caused too big, and is susceptible to block, therefore, perpendicular to water (flow) direction, several are set During lamina affixad 4, the mesh aperture for being located at most upstream lamina affixad 4 should select larger aperture.
Example IV
It is the structural representation of embodiments of the invention four referring to Fig. 4.The present embodiment mainly exists with the difference of embodiment three In, wherein the length direction of lamina affixad 4 no longer with water stream channel P in water (flow) direction vertical relation, but incline be in an angle θ, wherein 0 90 ° of < θ <.It is in the present embodiment 20 °.
Wherein, lamina affixad 4 is preferably grid shaped board chip architecture, and further, structure as shown in Figure 5 C, in attachment Plate 4 is provided with some mesh 43 ', and the aperture of these mesh 43 can be 1mm2~25mm2, additionally, also being set on the surface of lamina affixad 4 Many individual burrs or crotch 44 ' for protruding from the surface are put, the length that these burrs or crotch 44 ' protrude from surface is 0.5mm ~5mm.Lamina affixad 4 is made up of plastics or metal, can be formed in one or knitting forming, and growth of these materials to algae is harmless, And it is cheap and easy to get, compared with half dry type solid-state adhere-wall culture, without taking into account water retention property, mechanical strength and antibiotic property.By These mesh 43 ' and burr or crotch 44 ' further make the substantial amounts of filamentous algae of surface enrichment of lamina affixad 4.
Embodiment five
Referring to shown in Fig. 6 A, from unlike all of above embodiment, in the present embodiment, the top view of lamina affixad 4 is not In straight line type, but in wave linear shape.Found in experimentation, filamentous algae is in nutrient solution 2 with the stream of nutrient solution 2 It is dynamic, it is easy to which that enrichment on some surfaces is got up, particularly in bending or corner, the speed and thickness of enrichment all quickly, and And when needing to be discharged in new culture medium, it is only necessary to the angle with nutrient solution flow direction is gently rocked or changed in nutrient solution Degree, it is possible to which the filamentous algae that will be enriched with thereon quickly is discharged into nutrient solution, or can just be taken off by shaking full wafer algae mud Fall and be available for Collection utilization.Therefore, in the present embodiment, the top view presentation waveform line style of lamina affixad 4, and lamina affixad 4 Can be shaped after bending back and forth by latticed plate and obtained, it is either long or short to be similarly formed several on the surface of latticed plate Burr or crotch 44, or thrust etc., can play a part of to catch and be enriched with filamentous algae.Lamina affixad in the present embodiment Lamina affixad in any embodiment of 4 one~embodiment of alternate embodiments five.
Additionally, lamina affixad 4 can also be embodied as its top view in the zigzag of Fig. 6 B, Fig. 6 C it is concavo-convex linear, Fig. 6 D Turning type;Or a more anxious step is set to " rich " font such as Fig. 6 E, Fig. 6 F, Fig. 6 G fishbones, and for the purposes of improving attachment filament The ability of shape algae, each lamina affixad 4 is to be formed using plastic cord, nylon yarn, metal wire knitted, and surface also has hair different in size Thorn etc..In actual experimental study, a kind of lamina affixad 4 can be used, be that a predetermined shape is bent to by layer of metal waffle slab Afterwards, a hook side for VELCRO being fixed again on its surface and being constituted, experiment effect is more satisfactory (referring to Fig. 7).
The above, representational specific embodiment only of the invention, but protection scope of the present invention is not limited to This, any one skilled in the art the change that can readily occur in or replaces in the technical scope of present disclosure Change, should all be included within the scope of the present invention.

Claims (11)

1. a kind of culture apparatus of filamentous algae, it is characterized in that, the culture apparatus includes a filamentous algae culture pond, in the training Submerged in foster pond and at least one water stream channel, the training in the water stream channel are formed at least one lamina affixad, the culture pond Nutrient solution is flowed towards a predetermined direction, and the lamina affixad is located in the water stream channel of the nutrient solution, thread in the nutrient solution Algae is constantly enriched on the lamina affixad with the flowing of nutrient solution.
2. the culture apparatus of a kind of filamentous algae according to claim 1, it is characterized in that, the culture pond is raceway pond, institute State and be provided with the middle of runway a demarcation strip, the water stream channel is formed at the dividing plate surrounding;Or the culture pond is circle Pond, is provided with a demarcation strip in circular pond, and the water stream channel is formed at the dividing plate surrounding.
3. the culture apparatus of a kind of filamentous algae according to claim 1, it is characterized in that, the nutrient solution warp in the culture pond Motor drives an agitating paddle to drive or driven through gas explosion, the nutrient solution in culture pond is flowed towards a predetermined direction.
4. the culture apparatus of a kind of filamentous algae according to claim any one of 1-3, it is characterized in that, the lamina affixad is vertical In the direction of the nutrient solution thickness is immersed in nutrient solution, the flowing side of the length direction of the lamina affixad and the nutrient solution To parallel, water stream channel is partitioned into multiple weep passages.
5. the culture apparatus of a kind of filamentous algae according to claim any one of 1-3, it is characterized in that, the lamina affixad is vertical In the direction of the nutrient solution thickness is immersed in nutrient solution, the flowing side of the length direction of the lamina affixad and the nutrient solution To at an angle, the angle is less than or equal to 90 ° more than 0 °.
6. the culture apparatus of a kind of filamentous algae according to claim 4 or 5, it is characterized in that, the lamina affixad is latticed Plate, thereon with several mesh, the mesh can flow through for nutrient solution.
7. the culture apparatus of a kind of filamentous algae according to claim 6, it is characterized in that, the lamina affixad is contacted with nutrient solution Surface there are some burrs or crotch for protruding from the surface.
8. the culture apparatus of a kind of filamentous algae according to claim 7, it is characterized in that, the burr or crotch protrude from this The length on surface is 0.5mm~5mm.
9. the culture apparatus of a kind of filamentous algae according to claim 6, it is characterized in that, the top view of the lamina affixad is in turning Angle-style, wave linear shape, sawtooth are linear or concavo-convex linear.
10. the culture apparatus of a kind of filamentous algae according to claim 6, it is characterized in that, the top view of the lamina affixad is in " rich " font or " fish-bone " shape.
A kind of 11. cultural methods of filamentous algae, it is characterized in that, in a culture pond for being inoculated with filamentous algae, assign at least one The individual lamina affixad that can be enriched with filamentous algae.
CN201710232865.4A 2017-04-11 2017-04-11 The culture apparatus of filamentous algae Pending CN106906123A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710232865.4A CN106906123A (en) 2017-04-11 2017-04-11 The culture apparatus of filamentous algae

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710232865.4A CN106906123A (en) 2017-04-11 2017-04-11 The culture apparatus of filamentous algae

Publications (1)

Publication Number Publication Date
CN106906123A true CN106906123A (en) 2017-06-30

Family

ID=59195010

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710232865.4A Pending CN106906123A (en) 2017-04-11 2017-04-11 The culture apparatus of filamentous algae

Country Status (1)

Country Link
CN (1) CN106906123A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107723214A (en) * 2017-11-13 2018-02-23 烟台大学 Plain type raceway pond and its application method for microdisk electrode
CN107746801A (en) * 2017-09-25 2018-03-02 丽江程海保尔生物开发有限公司 The defoaming device and application method of a kind of cultivating spirulina
CN109355170A (en) * 2018-11-27 2019-02-19 重庆滂渤农业科技有限公司 Yellow silk algae culturing device and cultural method
WO2021068604A1 (en) * 2019-10-11 2021-04-15 孙旭阳 Method and device for adherent culture of microalgae in thin liquid layer by movement and mechanical stirring

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006230211A (en) * 2005-02-22 2006-09-07 Nikken Sohonsha Corp Culturing apparatus and culturing method for filamentous microalga
CN102206570A (en) * 2010-03-31 2011-10-05 中国科学院青岛生物能源与过程研究所 Apparatus and cultivating method for scaled cultivation of microalgae
CN102382755A (en) * 2011-07-28 2012-03-21 中国科学院青岛生物能源与过程研究所 Microalgae culturing device and microalgae culturing method
CN103184139A (en) * 2011-12-27 2013-07-03 新奥科技发展有限公司 Light reaction apparatus
US20130244309A1 (en) * 2012-03-16 2013-09-19 University Of Georgia Research Foundation, Inc. Algal Floway (AGF) System for Economical and Efficient Harvesting of Algal Biomass and Method of Use
CN103421677A (en) * 2013-04-26 2013-12-04 中国科学院过程工程研究所 Baffle plate public pool used for large-scale cultivation of microalgae
WO2013186626A1 (en) * 2012-06-12 2013-12-19 Arvind Mallinath Lali Raceway pond system for increased biomass productivity
CN103960117A (en) * 2013-01-29 2014-08-06 中国科学院青岛生物能源与过程研究所 Method for preparing tribonema biological oil and tribonema biological oil prepared by method
US20150125946A1 (en) * 2012-05-07 2015-05-07 Institute of Process Engineering ,Chinese Academy of Sciences Horizontal Immersion Cover Type Carbon Supplement Device and Carbon Supplement Method Used for Microalgae Culture in Open Pond
CN204824851U (en) * 2015-07-16 2015-12-02 中国海洋大学 Multidirectional torrent, little algae system of cultivateing in high -efficient novel runway pond of mixing
CN105733930A (en) * 2016-05-05 2016-07-06 中国科学院广州能源研究所 Rotating disc type photobioreactor for microalgae large-scale culture

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006230211A (en) * 2005-02-22 2006-09-07 Nikken Sohonsha Corp Culturing apparatus and culturing method for filamentous microalga
CN102206570A (en) * 2010-03-31 2011-10-05 中国科学院青岛生物能源与过程研究所 Apparatus and cultivating method for scaled cultivation of microalgae
CN102382755A (en) * 2011-07-28 2012-03-21 中国科学院青岛生物能源与过程研究所 Microalgae culturing device and microalgae culturing method
CN103184139A (en) * 2011-12-27 2013-07-03 新奥科技发展有限公司 Light reaction apparatus
US20130244309A1 (en) * 2012-03-16 2013-09-19 University Of Georgia Research Foundation, Inc. Algal Floway (AGF) System for Economical and Efficient Harvesting of Algal Biomass and Method of Use
US20150125946A1 (en) * 2012-05-07 2015-05-07 Institute of Process Engineering ,Chinese Academy of Sciences Horizontal Immersion Cover Type Carbon Supplement Device and Carbon Supplement Method Used for Microalgae Culture in Open Pond
WO2013186626A1 (en) * 2012-06-12 2013-12-19 Arvind Mallinath Lali Raceway pond system for increased biomass productivity
CN103960117A (en) * 2013-01-29 2014-08-06 中国科学院青岛生物能源与过程研究所 Method for preparing tribonema biological oil and tribonema biological oil prepared by method
CN103421677A (en) * 2013-04-26 2013-12-04 中国科学院过程工程研究所 Baffle plate public pool used for large-scale cultivation of microalgae
CN204824851U (en) * 2015-07-16 2015-12-02 中国海洋大学 Multidirectional torrent, little algae system of cultivateing in high -efficient novel runway pond of mixing
CN105733930A (en) * 2016-05-05 2016-07-06 中国科学院广州能源研究所 Rotating disc type photobioreactor for microalgae large-scale culture

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TIANZHONG LIU等: "Attached cultivation technology of microalgae for efficient biomass feedstock production", 《BIORESOURCE TECHNOLOGY》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107746801A (en) * 2017-09-25 2018-03-02 丽江程海保尔生物开发有限公司 The defoaming device and application method of a kind of cultivating spirulina
CN107723214A (en) * 2017-11-13 2018-02-23 烟台大学 Plain type raceway pond and its application method for microdisk electrode
CN109355170A (en) * 2018-11-27 2019-02-19 重庆滂渤农业科技有限公司 Yellow silk algae culturing device and cultural method
WO2021068604A1 (en) * 2019-10-11 2021-04-15 孙旭阳 Method and device for adherent culture of microalgae in thin liquid layer by movement and mechanical stirring

Similar Documents

Publication Publication Date Title
Xu et al. Microalgal bioreactors: challenges and opportunities
CN102206570B (en) Apparatus and cultivating method for scaled cultivation of microalgae
Chaumont Biotechnology of algal biomass production: a review of systems for outdoor mass culture
Grivalský et al. Development of thin-layer cascades for microalgae cultivation: milestones
Zittelli et al. Photobioreactors for mass production of microalgae
WO2017190504A1 (en) Rotating disc type photobioreactor for microalgae large-scale cultivation
CN106906123A (en) The culture apparatus of filamentous algae
US8809041B2 (en) Photo bioreactor with light distributor and method for the production of a photosynthetic culture
Koller Design of closed photobioreactors for algal cultivation
US20100167381A1 (en) Photo Bioreactor with Light Distributor and Method for the Production of a Photosynthetic Culture
Egbo et al. Photobioreactors for microalgae cultivation–an overview
Liu et al. Biomass productivity of Scenedesmus dimorphus (Chlorophyceae) was improved by using an open pond–photobioreactor hybrid system
WO2013186626A1 (en) Raceway pond system for increased biomass productivity
KR20160000206A (en) Photo-Bioreactor for Cultivation of Photosynthesis Autotrophic Organisms
CA3141655C (en) Methods and systems for efficient bioreactor mixing and light utilization embodying low process energy and scalability
CN104521726B (en) Grading breeding device for breeding through biogas slurry and breeding method of grading breeding device
CN107828636A (en) A kind of microalgae culturing device adopting
İhsan Types of microalgae cultivation photobioreactors and production process of microalgal biodiesel as alternative fuel
CN104726321A (en) Runway type bioreactor applicable to sunshine factorization
KR101663108B1 (en) Light Tube for Photo-Bioreactor for Cultivation of Photosynthesis Autotrophic Organisms
Nigam et al. Cultivation and production techniques of marine algae
Carlozzi Closed photobioreactor assessments to grow, intensively, light dependent microorganisms: a twenty-year Italian outdoor investigation
CN201485462U (en) Accelerator for culturing microalgae
Griffiths 5 Microalgal Cultivation
El-Baz et al. Pilot scale of microalgal production using photobioreactor

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
CB02 Change of applicant information

Address after: 100034 -6 international investment building, 6 Fuchengmen North Street, Xicheng District, Beijing.

Applicant after: National Development Investment Group Co.,Ltd.

Applicant after: CHINA ELECTRONICS ENGINEERING DESIGN INSTITUE

Address before: 100034 block A, -6 international investment building, 6 Fuchengmen North Street, Xicheng District, Beijing.

Applicant before: STATE DEVELOPMENT AND INVESTMENT Corp.

Applicant before: CHINA ELECTRONICS ENGINEERING DESIGN INSTITUE

CB02 Change of applicant information
TA01 Transfer of patent application right

Effective date of registration: 20181106

Address after: 100034 147 Xizhimen South Street, Xicheng District, Beijing

Applicant after: SDIC BIOTECHNOLOGY INVESTMENT Co.,Ltd.

Address before: 100034 -6 international investment building, 6 Fuchengmen North Street, Xicheng District, Beijing.

Applicant before: National Development Investment Group Co.,Ltd.

Applicant before: CHINA ELECTRONICS ENGINEERING DESIGN INSTITUE

TA01 Transfer of patent application right
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20170630

RJ01 Rejection of invention patent application after publication