CN107723214A - Plain type raceway pond and its application method for microdisk electrode - Google Patents
Plain type raceway pond and its application method for microdisk electrode Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention belongs to photobioreactor field, and in particular to plain type raceway pond and its application method for microdisk electrode.Including raceway pond liner and raceway pond framework, wherein raceway pond framework includes straight channel wall, curved flow path wall, flow blocking wall, agitating paddle, variable speed electric motors, particularly, strut and liquid impellers；The straight channel wall and curved flow path wall and the circulating water pool framework of flow blocking wall composition raceway pond；The raceway pond framework of the present invention, structure is simple, solid, and convenient dismounting, easily amplification, can determine corresponding size according to actual place；The raceway pond liner of the present invention is a complete tube-in-tube structure, will not produce fold during use, it is not required that bonding, leakage can be effectively avoided, consisting of food grade materials, heavy metal pollution risk when can avoid using cement or geomembrane；The liquid impellers of the present invention can coordinate agitating paddle to use, and have the characteristics of significantly reducing liquid mixing energy consumption, while avoid frustule from precipitating, so as to be advantageous to improve area yield.
Description
Technical field
The invention belongs to photobioreactor field, and in particular to a kind of plain type raceway pond for microdisk electrode and its
Application method.
Background technology
Microalgae is a kind of phototroph, can pass through fixed CO2Produce multi-chemical.Some can be used for producing
Aliphatic hydrocarbon, as Wild Vitis species produce hydrocarbon amount up to the 15%~75% of dry cell weight；Some can be used for accumulating glycogen, and some can be used for accumulating
Tired glycerine, the fat content of many microalgaes are the important source materials for producing biodiesel up to more than the 30% of dry weight.Yin Qisheng again
Long speed is fast, the advantage of cultivating etc. can be carried out using waste water and gas, worldwide of increased attention.
At present, the culture of microalgae is mainly carried out in bioreactor, and the photo-biological for being currently used for microdisk electrode is anti-
Device is answered mainly there are open and closed two types.It is open to refer to using open pond culture apparatus, such as raceway pond, circle
Pond, this kind of reactor have the features such as simple to operate, low investment, in the industrialized production of spirulina, chlorella and salt algae
Be applied widely (Liu Tianzhong, Zhang Wei, Wang Junfeng micro-algae large-scale culture technique progress life sciences, 2014,26
(005):509-522；Ben-Amotz A,Avron M.The biotechnology of mass culturing
Dunaliella for products of commercial interest.Algal and Cyanobacterial
Biotechnology,1989,91-114；Pulz O.Photobioreactor:production systems for
phototrophic microorganisms.Applied Microbiology and Biotechnology,2001,57
(3):287-293).The closed bioreactor for referring to the closed using different structure, such as gas-lifting type, stirring-type, pipe
Formula etc., seeding tank (the Tredici M available for production high value products (such as medicine or health products) or as open pond culture
R.Massproduction of microalgae:photobioreactors.Handbook of microalgal
culture.Biotechnology and Applied Phycology,2004,(1)178-214；Lehr F,Posten
C.Closed photobioreactors as tools for biofuel production.Current opinion in
Biotechnology,2009,20(3):280-285.)。
In traditional Large-scale microalgae incubation, raceway pond is Typical Representative, and is most widely used in the world
Microalgae culture system.Raceway pond is usually to be constructed by sand or cement, needs plastic film mulch to prevent leakage in incubation, also may be used
To add impervious barrier at cement pit bottom.In China Inner Mongol area, the opening pond used in both culturing microalgae is usually the race surrounded by sand
Road formula water channel, laying plastic, generally polyethylene or nylon plastic(s) film are needed in production process.Due to this type film very
Fragility, easily scratched by rubble, sharp weapon etc., the phenomenon of leakage happens occasionally during microdisk electrode, and frequently changes mulch film and expend
Manpower and materials and cause white pollution.The ground or half ground raceway pond constructed by cement are more conventional in southern region of China,
And geomembrane can be laid after the completion of bottom of pond add impervious barrier, or cement pit construction typically in civil engineering, material is generally height
Density Polyethylene Resin.But because nutrient solution directly contacts cement or geomembrane, content of beary metal in microalgae cell is caused to surpass
Target increased risk.With further attention of the people to product quality, microdisk electrode should enter in an environmental protection, safe system
OK.
In addition, the flowing of nutrient solution typically drives by agitator (impeller) in raceway pond, agitator is generally arranged at runway
In the straight channel in pond, make microalgae shuttling movement, uniformly see light.Raceway pond typically has dead band at curved runner, at dead band
Microalgae is easy to mix uneven, causes cell precipitation, influences the growth of microalgae.The mean depth of nutrient solution is generally 20~
30cm, under this condition, because the penetration depth of light is limited, it is difficult to reach High Density Cultivation, in general culture density is no more than
1g/L, it is most of in 0.5g/L or so.Increase mixing speed can cause current to accelerate, and reduce dead at the curved runner of raceway pond
Area, at the same be sufficiently mixed nutrient solution, frustule receives illumination in turn, be advantageous to increase the total yield.But drive nutrient solution
The consumed energy consumption of flowing, mixing will greatly increase.
For the construction material and method of raceway pond, correlative study is not a lot.Han Feifei etc. uses polypropylene plastics
It machined 1.35m2Plastic track pond (Han Feifei, thesis for the doctorate, the chlorella light autotrophy culture using high lipid-producing as target
Process optimization and preliminary amplification, East China University of Science, 2013, p84-85.), the side that Sun Zhongliang etc. is welded using stainless steel iron plate
Method machined 2m2Irony raceway pond (Sun Zhongliang, thesis for the doctorate, the absorption enhancement and cigarette of low concentration of carbon dioxide culture microalgae
Road gas component modulation, university of the Chinese Academy of Sciences, 2015, p55.).But there is cost height in above-mentioned material, it is impossible to which amplification lacks
Point.The problems such as mixing for nutrient solution in raceway pond and stirring energy consumption, national inventing patent ZL20109100.4 discloses one
Kind opens pond and its application method and purposes for the low head of large-scale culturing micro-algae, described according to the opening pond of this invention
Open pond includes agitator, wherein, rotating mixer, in the height highest of the bottom of pond in the algae solution exit of agitator, with algae solution
The bottom of pond difference in height of porch is less than 5cm, and remaining runner is all lower slope section, and this invention is advantageously implemented thin layer cultivation, improves
Algae cell density, reduce the energy consumption of water body driving；China national practical new-type patent ZL201420768663.3 disclose deflector and
Using its raceway pond, according to this utility model, deflector is set in raceway pond curved runner section, is fixedly connected on the deflector
Have for supporting the deflector and forming it into the support frame of circular shape, the both ends of the main backstop are separately fixed at institute
On the top edge for stating deflector both ends, this utility model advantageously reduces the mixing dead band at curved runner.But above-mentioned improvement increases
The complexity of runway pool structure is added, the quantities of outdoor construction is bigger than normal, is unfavorable for the facility of control and the operation of cost.
The content of the invention
It is an object of the invention to overcome leakage existing for traditional raceway pond construction method and heavy metal pollution risk big
Phenomenon, the and the problem of material such as plastics, iron plate is not easy to amplify, there is provided it is a kind of can amplify and cultivate environmental protection, safety is used for
The low cost simple formula raceway pond of microdisk electrode.
Another object of the present invention is to provide the method using above-mentioned raceway pond culture microalgae.
Idea of the invention is that weld to form raceway pond framework using stainless steel tube and stainless steel mesh sheet, instead of traditional digging
The raceway pond method of construction of canal and joints cement；Bonded using the PVC grid cloth of food-grade and to form raceway pond liner and replace poly- second
Alkene film and geomembrane；Inner lining film and framework form closed circulation pond, are combined using liquid impellers are installed at curved runner
The mode of agitating paddle, it is common to promote liquid flowing.
The technical solution adopted by the present invention is：A kind of plain type raceway pond for microdisk electrode, including raceway pond liner
With raceway pond framework (Fig. 1), wherein raceway pond framework includes straight channel wall 1, curved flow path wall 2, flow blocking wall 3, agitating paddle 4, speed change
Motor 5, strut 6 and liquid impellers 7 (Fig. 2)；The straight channel wall 1 forms following for raceway pond with curved flow path wall 2 and flow blocking wall 3
Ring pond framework；The strut 6 is used to connect flow blocking wall 3 and straight channel wall 1 and curved flow path wall 2, positioned at the top of raceway pond and
Bottom of pond, length form curved equal to the width or curved flow path wall 2 of the straight channel that straight channel wall 1 forms with flow blocking wall 3 with flow blocking wall 3
The radius of runner；The agitating paddle 4 is located in straight channel, and its one end is fixed on flow blocking wall 3, and the other end connects with variable speed electric motors, particularly 5
Connect, rotated under the drive of variable speed electric motors, particularly 5 so as to promote liquid to flow；The liquid impellers 7 be separately mounted to curved runner with
The joining place of straight channel is at angle theta with liquid flow direction nearby or in curved runner, wherein 0<θ<90。；The race
Road pond liner is different from the plastic sheeting for sand water channel, and also different from the geomembrane for cement pit, but one complete
Tube-in-tube structure, fold will not be produced during use, it is not required that bonding, opening can be formed under the support of peripheral frame
Pond is closed, material is food-grade PVC grid Tarpaulings, and its size is consistent with circulating water pool framework；The plain type runway
The erection sequence in pond is：(1) bottom margin of straight channel wall 1, curved flow path wall 2 and flow blocking wall 3 is fixed using strut 6；(2) will run
Road pond liner is fixed on circulating water pool framework；(3) the upper of straight channel wall 1, curved flow path wall 2 and flow blocking wall 3 is fixed using strut 6
Portion edge；(4) in straight channel on agitating paddle 4 be installed and be connected to variable speed electric motors, particularly 5；(5) in curved runner and curved runner
Liquid impellers 7 are installed respectively with straight channel joining place.
According to the plain type raceway pond of the present invention, wherein, the racetrack liner is by drawing point and self-sealed article or pipe collar
Mode fixed with circulating water pool framework.
According to the plain type raceway pond of the present invention, wherein, the PVC grid Tarpauling thickness is 0.5mm~0.9mm,
It is usually used in the project construction that outdoor swimming pool and amusement on water etc. directly contact with application on human skin, there is solid, low temperature resistant and high temperature, nothing
The characteristics of poison is harmless.
According to the plain type raceway pond of the present invention, wherein, the straight channel wall 1, curved flow path wall 2 are by stainless steel tube and not
Rust steel mesh piece is welded, highly 1.2~2 times for medium depth；The straight channel wall 1 and curved flow path wall 2 it is upper following
Edge is stainless steel tube, and centre is stainless steel mesh sheet；The stainless steel tube wall thickness is 1mm~2mm, a diameter of 10mm~50mm；Institute
It is 1mm~2mm to state stainless steel mesh sheet string diameter, and mesh specification is 2mm*2mm~10mm*10mm.
According to the present invention plain type raceway pond, wherein, the straight channel wall 1, curved flow path wall 2 by equal length component
Splicing is welded, and the single component length of straight channel wall 1 is 1m~5m；The curved single component of flow path wall 2 is the 1/6~1/2 of semicircle.
According to the plain type raceway pond of the present invention, wherein, the length and height and the length of straight channel wall 1 of the flow blocking wall 3
Spend and highly consistent, thickness is 10cm~50cm；The flow blocking wall 3 is equally welded by stainless steel tube and stainless steel mesh sheet,
Then it is spliced by the component of equal length, single component length is consistent with the single component length of straight channel wall 1；
According to the plain type raceway pond of the present invention, wherein, the flow blocking wall 3 is run with straight channel wall 1, the composition of curved flow path wall 2
Road formula framework, frame size are the arbitrary dimension of racetrack shape, and specification is consistent with raceway pond commonly used in the art.
According to the plain type raceway pond of the present invention, wherein, the strut 6 is that specification is wall thickness 1mm~2mm, a diameter of
10mm~50mm, length are equal to the stainless steel tube of the width either radius of curved runner of straight channel.
According to the present invention plain type raceway pond, wherein, the strut 6 have it is multigroup, its bearing be straight channel wall 1,
Curved flow path wall 2, the single component joining place of flow blocking wall 3,3 liang of the joining place and flow blocking wall of straight channel wall 1 and curved flow path wall 2
End；Described fixed form is fixed for welding or by screw-nut.
According to the plain type raceway pond of the present invention, wherein, the liquid impellers 7 are that the Shui nationality make unrestrained pump, work(with magnetic-type
Rate is 5W~60W, has the characteristics of Jing Yin, low energy consumption, low-shearing force；The liquid impellers 7 be arranged on culture medium liquid level with
Under, depth is 0.5~1.0 times of liquid depth.
According to the plain type raceway pond of the present invention, wherein, the liquid impellers 7 may be mounted at curved runner and straight channel
Optional position in junction or curved runner, also can be according to need when straight channel length is long or cultivated algae kind is easier to precipitation
To be arranged on the optional position of straight channel.
The present invention also provides a kind of method for being used to cultivate microalgae based on above-mentioned plain type raceway pond, it is characterised in that will
Racetrack framework combines to form recirculated water with raceway pond liner made of PVC grid cloth made of stainless steel and stainless steel mesh sheet
Pond, agitating paddle is arranged in straight channel and is connected with variable speed electric motors, particularly, liquid impellers are separately mounted in curved runner or
Curved runner and straight channel joining place, by liquid impellers and the collective effect of agitating paddle, micro-algae culture medium is promoted to press a constant speed
Rate v flows, and avoids the frond at flow blind angle from precipitating, and improves nutrient solution mixability.
According to the method for the microdisk electrode of the present invention, the flow rate v of the micro-algae culture medium is 10cm/s~100cm/
s。
According to the method for the microdisk electrode of the present invention, the collective effect of described liquid impellers and agitating paddle includes stirring
Oar is opened and liquid impellers part is opened or is all turned on.
Culture medium for cultivating microalgae can be the culture medium of any suitable micro algae growth well known in the art, such as
Zarrouk culture mediums, SM culture mediums, ASP2Culture medium, BG-11 culture mediums etc. or for certain algae special requirement
Culture medium.
The plain type raceway pond of the present invention and its method for cultivating microalgae, can be used in open, semi open model or room
The interior various microalgaes of pilot scale culture, including the various microalgaes such as spirulina, grid algae, haematococcus pluvialis, salt algae, chlorella, chlamydomonas.
The invention has the advantages that：(1) raceway pond framework of the invention, structure is simple, solid, convenient dismounting,
Easily amplification, can determine corresponding size according to actual place；(2) raceway pond liner of the invention is a complete sleeve knot
Structure, fold will not be produced during use, it is not required that bonding, leakage can be effectively avoided, consisting of food grade materials, can keep away
Exempt to use heavy metal pollution risk when cement or geomembrane；(3) liquid impellers of the invention can coordinate agitating paddle to use,
The characteristics of with liquid mixing energy consumption is significantly reduced, while avoid frustule from precipitating, so as to be advantageous to improve area yield.
Brief description of the drawings
Fig. 1 is the plain type raceway pond top view of the present invention；
Fig. 2 is the top view of the plain type raceway pond raceway pond framework of the present invention；
Fig. 3 is the schematic perspective view of the plain type raceway pond raceway pond framework of the present invention；
Fig. 4 is the plain type raceway pond longitudinal direction side view of the present invention；
Fig. 5 is the structure chart of composition raceway pond framework single component.
Reference：
1 straight channel wall；2 curved flow path walls；3 flow blocking walls；4 agitating paddles；5 variable speed electric motors, particularlies；6 struts；7 liquid impellers.
Embodiment
Embodiment 1
Area is used as 200m2Plain type raceway pond carry out spirulina culture.Specifically building mode is：Using diameter
3cm, wall thickness 2mm stainless steel tube and string diameter 2mm, mesh 6.4cm*6.4cm stainless steel mesh sheet are welded into straight channel wall, curved stream
The single component of road wall and flow blocking wall, strut specification are diameter 3cm, wall thickness 2mm；The wherein long 35m of straight channel, straight channel width
2.3m, curved runner radius 2.5m, flow blocking wall width 0.4m, raceway pond framework and liner are highly 0.6m, straight channel wall and flow blocking
The single component length of wall is 5m, the length of curved flow path wall single component is the 1/2 of semicircle；Using self-sealed article secure liner and frame
Frame, strut and straight channel wall, curved flow path wall and flow blocking wall are fixed in the form of screw-nut；Using 10W liquid impellers
The three curved runners and straight channel joining place of agitating paddle are mounted remotely from, setting angle θ is 45 °, and fitting depth is liquid depth
1/2.
Algae kind is blunt top spirulina (Spirulina Platensis), from aquatile institute of Chinese Academy of Sciences algae
Kind of storehouse, numbering is 439, and culture medium is Zarrouk culture mediums, and the mean depth of culture medium is 20cm in raceway pond, and frustule connects
Kind density 0.3g (dry weight)/L.Regulation variable speed electric motors, particularly make it that the flow velocity of nutrient solution in raceway pond is 15cm/s or so, opens simultaneously
Three liquid impellers.
The concentration of the nutritive salt of timing detection daily is simultaneously supplemented in time, and supplements a small amount of water to make up the evaporating loss of water.Hold
Continuous culture 7 days, algae cell density reaches 0.7g (dry weight)/L, and the yield of unit area frustule reaches 13.3g (dry weight)/m2·
d.Obtain the composition and content and document report basic one of the conventional ingredient of spirulina, amino acid, aliphatic acid and carotenoid
Cause, do not detect the toxic heavy-metal elements such as mercury, chromium.Stirring power consumption and liquid impellers power consumption are total up to 1.10W/m2。
And same raceway pond is used, regulation variable speed electric motors, particularly causes the flow velocity of nutrient solution in raceway pond to be 25cm/s or so,
Do not open three liquid impellers, under similarity condition, the yield of the unit area frustule of culture 7 days for 13.1g (dry weight)/
m2D, and it is 1.53W/m to stir power consumption2。
Embodiment 2
Other are the same as embodiment 1.Algae kind is dimorphism grid algae (Scenedesmus dimorphus), from Chinese Academy of Sciences's water
Raw biological institute's algae kind storehouse, numbering is 496.With BG11 medium culture dimorphism grid algaes, the depth of nutrient solution is 20cm, and algae is thin
Born of the same parents' inoculum density 0.3g (dry weight)/L.Regulation variable speed electric motors, particularly make it that the flow velocity of nutrient solution in raceway pond is 20cm/s or so, simultaneously
Open three liquid impellers.
The concentration of the nutritive salt of timing detection daily is simultaneously supplemented in time, is regularly passed through CO2, and a small amount of water is supplemented to make up water
Evaporating loss.Persistently cultivate 7 days, algae cell density reaches 0.65g (dry weight)/L, and the yield of unit area frustule reaches
10.0g (dry weight)/m2·d.Obtain the composition of the conventional ingredient of dimorphism grid algae, amino acid, aliphatic acid and carotenoid and contain
Amount is basically identical with document report, does not detect the toxic heavy-metal elements such as mercury, chromium.Stir power consumption and liquid impellers power consumption altogether
For 1.36W/m2。
And same raceway pond is used, regulation variable speed electric motors, particularly causes the flow velocity of nutrient solution in raceway pond to be 30cm/s or so,
Do not open three liquid impellers, under similarity condition, the yield of the unit area frustule of culture 7 days for 10.1g (dry weight)/
m2D, and it is 1.77W/m to stir power consumption2。
Embodiment 3
Area is used as 600m2Plain type raceway pond carry out spirulina culture.Specifically building mode is：Using diameter
3cm, wall thickness 2mm stainless steel tube and string diameter 2mm, mesh 6.4cm*6.4cm stainless steel mesh sheet are welded into straight channel wall, curved stream
The single component of road wall and flow blocking wall, strut specification are diameter 3cm, wall thickness 2mm；The wherein long 115m of straight channel, straight channel width
2.3m, curved runner radius 2.5m, flow blocking wall width 0.4m, raceway pond framework and liner are highly 0.6m, straight channel wall and flow blocking
The single component length of wall is 5m, the length of curved runner single component is the 1/2 of semicircle；Using self-sealed article secure liner and frame
Frame, strut and straight channel wall, curved flow path wall and flow blocking wall are fixed in the form of screw-nut；Using 30W liquid impellers
The three curved runners and straight channel joining place of agitating paddle are mounted remotely from, setting angle θ is 60 °, and fitting depth is liquid depth
1/2.
Algae kind is blunt top spirulina (Spirulina Platensis), from aquatile institute of Chinese Academy of Sciences algae
Kind of storehouse, numbering is 439, and culture medium is Zarrouk culture mediums, and the mean depth of culture medium is 20cm in raceway pond, and frustule connects
Kind density 0.3g (dry weight)/L.Regulation variable speed electric motors, particularly make it that the flow velocity of nutrient solution in raceway pond is 15cm/s or so, opens simultaneously
Three liquid impellers.
The concentration of the nutritive salt of timing detection daily is simultaneously supplemented in time, and supplements a small amount of water to make up the evaporating loss of water.Hold
Continuous culture 12 days, algae cell density reach 0.9g (dry weight)/L, the yield of unit area frustule reach 10.2g (dry weight)/
m2·d.Obtain the composition and content and document report base of the conventional ingredient of spirulina, amino acid, aliphatic acid and carotenoid
This is consistent, does not detect the toxic heavy-metal elements such as mercury, chromium.Stirring power consumption and liquid impellers power consumption are total up to 1.70W/m2。
And same raceway pond is used, regulation variable speed electric motors, particularly causes the flow velocity of nutrient solution in raceway pond to be 25cm/s or so,
Do not open three liquid impellers, under similarity condition, the yield of the unit area frustule of culture 9 days for 9.7g (dry weight)/
m2D, and it is 1.83W/m to stir power consumption2。
Embodiment 4
Other are the same as embodiment 3.Algae kind is dimorphism grid algae (Scenedesmus dimorphus), from Chinese Academy of Sciences's water
Raw biological institute's algae kind storehouse, numbering is 496.With BG11 medium culture dimorphism grid algaes, the depth of nutrient solution is 20cm, and algae is thin
Born of the same parents' inoculum density 0.3g (dry weight)/L.Regulation variable speed electric motors, particularly make it that the flow velocity of nutrient solution in raceway pond is 20cm/s or so, simultaneously
Open three liquid impellers.
The concentration of the nutritive salt of timing detection daily is simultaneously supplemented in time, is regularly passed through CO2, and a small amount of water is supplemented to make up water
Evaporating loss.Persistently cultivate 9 days, algae cell density reaches 0.88g (dry weight)/L, and the yield of unit area frustule reaches
12.8g (dry weight)/m2·d.Obtain the composition of the conventional ingredient of dimorphism grid algae, amino acid, aliphatic acid and carotenoid and contain
Amount is basically identical with document report, does not detect the toxic heavy-metal elements such as mercury, chromium.Stir power consumption and liquid impellers power consumption altogether
For 1.76W/m2。
And same raceway pond is used, regulation variable speed electric motors, particularly causes the flow velocity of nutrient solution in raceway pond to be 30cm/s or so,
Do not open three liquid impellers, under similarity condition, the yield of the unit area frustule of culture 7 days for 10.1g (dry weight)/
m2D, and it is 2.05W/m to stir power consumption2。
Claims (10)
- A kind of 1. plain type raceway pond for microdisk electrode, it is characterised in that：The raceway pond includes raceway pond liner and race Road pond framework, wherein raceway pond framework include straight channel wall (1), curved flow path wall (2), flow blocking wall (3), agitating paddle (4), speed change electricity Machine (5), strut (6) and liquid impellers (7)；The straight channel wall (1) and curved flow path wall (2) and flow blocking wall (3) composition runway The circulating water pool framework in pond；The strut (6) is used to connect flow blocking wall (3) and straight channel wall (1) and curved flow path wall (2), is located at The top of raceway pond and bottom of pond, length are equal to the width of straight channel wall (1) and the straight channel of flow blocking wall (3) composition or curved flow path wall (2) radius of the curved runner formed with flow blocking wall (3)；The agitating paddle (4) is located in straight channel, and its one end is fixed on flow blocking wall (3) on, the other end is connected with variable speed electric motors, particularly (5), is rotated under the drive of variable speed electric motors, particularly (5) so as to promote liquid to flow；It is described Liquid impellers (7) are separately mounted near curved runner and the joining place of straight channel or in curved runner, with liquid flow Dynamic direction is at angle theta, wherein 0<θ<90。；The raceway pond liner is a complete tube-in-tube structure, will not produce pleat during use Wrinkle, it is not required that bonding, the closing pond of opening under the support of peripheral frame, can be formed, material is food-grade PVC grid Tarpauling, its size are consistent with circulating water pool framework.
- 2. it is used for the plain type raceway pond of microdisk electrode according to claim 1, it is characterised in that：The racetrack liner leads to Cross drawing point and the mode of self-sealed article or pipe collar is fixed with circulating water pool framework；The PVC grid Tarpauling thickness is 0.5mm ~0.9mm.
- 3. it is used for the plain type raceway pond of microdisk electrode according to claim 1, it is characterised in that：The straight channel wall (1), Curved flow path wall (2) is welded by stainless steel tube and stainless steel mesh sheet, highly 1.2~2 times for medium depth；It is described straight The lower edges of flow path wall (1) and curved flow path wall (2) are stainless steel tubes, and centre is stainless steel mesh sheet；The stainless steel tube wall thickness For 1mm~2mm, a diameter of 10mm~50mm；The stainless steel mesh sheet string diameter is 1mm~2mm, mesh specification be 2mm*2mm~ 10mm*10mm；The straight channel wall (1), curved flow path wall (2) are welded by the component splicing of equal length, straight channel wall (1) Single component length is 1m~5m；Curved flow path wall (2) single component is the 1/6~1/2 of semicircle.
- 4. it is used for the plain type raceway pond of microdisk electrode according to claim 1, it is characterised in that：The flow blocking wall (3) Length and height and the length of straight channel wall (1) and highly consistent, thickness is 10cm~50cm；The flow blocking wall (3) is by stainless Steel pipe and stainless steel mesh sheet are welded, and are then spliced by the component of equal length, single component length and straight channel wall (1) single component length is consistent.
- 5. it is used for the plain type raceway pond of microdisk electrode according to claim 1, it is characterised in that：The flow blocking wall (3) with Straight channel wall (1), curved flow path wall (2) composition racetrack framework, frame size are the arbitrary dimension of racetrack shape, specification and sheet The conventional raceway pond in field is consistent.
- 6. it is used for the plain type raceway pond of microdisk electrode according to claim 1, it is characterised in that：The strut (6) is rule Lattice are wall thickness 1mm~2mm, a diameter of 10mm~50mm, and length is equal to the radius of the width either curved runner of straight channel not Become rusty steel pipe；The strut (6) have it is multigroup, its bearing be straight channel wall (1), curved flow path wall (2), flow blocking wall (3) it is single The joining place and flow blocking wall (3) both ends of component joining place, straight channel wall (1) with curved flow path wall (2)；Described fixed form is Welding is fixed by screw-nut.
- 7. it is used for the plain type raceway pond of microdisk electrode according to claim 1, it is characterised in that：The liquid impellers (7) unrestrained pump is made with magnetic-type for the Shui nationality, power is 5W~60W, has the characteristics of Jing Yin, low energy consumption, low-shearing force；The liquid Propeller (7) is arranged on below culture medium liquid level, and depth is 0.5~1.0 times of liquid depth, may be mounted at curved runner with it is straight Optional position in runner junction or curved runner, also can root when straight channel length is long or cultivated algae kind is easier to precipitation According to the optional position needed installed in straight channel.
- A kind of 8. method of the plain type raceway pond culture microalgae described in claim 1, it is characterised in that：By stainless steel and stainless Racetrack framework made of steel mesh piece is combined to form circulating water pool with raceway pond liner made of PVC grid cloth, and agitating paddle is pacified It is connected in straight channel and with variable speed electric motors, particularly, liquid impellers is separately mounted in curved runner or curved runner and straight channel Joining place, by liquid impellers and the collective effect of agitating paddle, promote micro-algae culture medium to be flowed by given pace v, avoid flowing Frond precipitation at dynamic dead angle, improves nutrient solution mixability.
- A kind of 9. method based on plain type raceway pond culture microalgae described in claim 8, it is characterised in that：The microdisk electrode The flow rate v of base is 10cm/s~100cm/s.
- A kind of 10. method based on plain type raceway pond culture microalgae described in claim 8, it is characterised in that：Described liquid The collective effect of propeller and agitating paddle includes agitating paddle unlatching and liquid impellers part is opened or is all turned on.
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| CN201711112380.8A CN107723214A (en) | 2017-11-13 | 2017-11-13 | Plain type raceway pond and its application method for microdisk electrode |
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| CN201711112380.8A CN107723214A (en) | 2017-11-13 | 2017-11-13 | Plain type raceway pond and its application method for microdisk electrode |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113575494A (en) * | 2021-08-20 | 2021-11-02 | 徐州工程学院 | Fish and little algae cultivation circulation runway pond |
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| CN202465679U (en) * | 2012-01-19 | 2012-10-03 | 丽江程海保尔生物开发有限公司 | Ventilating and stirring device for spirulina breeding greenhouse |
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