JP2012023979A - Raceway-pond type culture tank - Google Patents

Raceway-pond type culture tank Download PDF

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JP2012023979A
JP2012023979A JP2010163387A JP2010163387A JP2012023979A JP 2012023979 A JP2012023979 A JP 2012023979A JP 2010163387 A JP2010163387 A JP 2010163387A JP 2010163387 A JP2010163387 A JP 2010163387A JP 2012023979 A JP2012023979 A JP 2012023979A
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stirring means
carbon dioxide
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Akira Yoneda
晃 米田
Hiroaki Kato
宏明 加藤
Takashi Yamashita
孝 山下
Yukio Fukushima
幸生 福島
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Hitachi Plant Technologies Ltd
Euglena Co Ltd
Eneos Corp
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Euglena Co Ltd
JX Nippon Oil and Energy Corp
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    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
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    • C12M27/08Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis

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Abstract

PROBLEM TO BE SOLVED: To provide a raceway-pond type culture tank capable of controlling the culture cost and permitting dissolution of a sufficient amount of carbon dioxide in a culture solution even in a shallow depth of water.SOLUTION: The raceway-pond type culture tank includes: a stirring means in a culture channel 8 through which the culture solution M flows, with the stirring means stirring the culture solution M; and a cover 1 for covering the stirring means and the liquid surface of the culture solution M is disposed above the stirring means. Carbon dioxide is fed to the space formed by the cover 1, and the carbon dioxide is dissolved in the culture solution M by the stirring means. By using the raceway-pond type culture tank, the culture cost can be controlled and a sufficient amount of carbon dioxide can be dissolved in the culture solution even in the shallow depth of water.

Description

本発明はレースウェイポンド型培養槽に関する。   The present invention relates to a raceway pond culture tank.

近年、二酸化炭素等の温室効果ガスの排出量を抑制する観点から、植物が吸収する二酸化炭素量と当該植物を焼却した際に生じる二酸化炭素の発生量とが等量であるという考え方、即ち「カーボンニュートラル」との考え方が浸透しつつある。このため、バイオマス資源由来の原料から生産される、所謂バイオマス燃料(例えばトリグリセリド等の脂質、エタノール等のアルコール等)の利用が以前にも増して注目されている。   In recent years, from the viewpoint of suppressing the emission of greenhouse gases such as carbon dioxide, the idea that the amount of carbon dioxide absorbed by the plant and the amount of carbon dioxide generated when the plant is incinerated is equivalent, that is, “ The concept of “carbon neutral” is spreading. For this reason, the use of so-called biomass fuels (for example, lipids such as triglycerides, alcohols such as ethanol) produced from raw materials derived from biomass resources has attracted more attention than before.

バイオマス燃料の原料となるバイオマス資源としては、例えば大豆、トウモロコシ、パーム等が挙げられるが、これらの可食性植物を上記のバイオマス資源として利用する場合、食糧不足への懸念や耕作地を確保するための森林破壊等という課題が生じることがある。そこで、上記のバイオマス資源に代わるものとして、池、湖沼等に広く生息する、光合成を行う原生生物等の光合成微生物は、植物と同様の光合成能を有し、水と二酸化炭素とから脂質、炭水化物等を生合成し、細胞内に数十質量%蓄積することが知られている。そして、この生産量は植物に比べて高く、具体的には、脂質、炭水化物等の生産量が高いといわれるパームの単位面積当たりで10倍以上であるため、上記の課題を解決するために非常に有効な手段である。   Examples of biomass resources that can be used as raw materials for biomass fuel include soybeans, corn, and palms. When these edible plants are used as the above biomass resources, there are concerns about food shortages and cultivated land. The problem of deforestation may occur. Therefore, as an alternative to the above biomass resources, photosynthetic microorganisms such as protozoa that live widely in ponds, lakes, etc. have the same photosynthetic ability as plants, from water and carbon dioxide to lipids, carbohydrates It is known that tens of mass% is accumulated in the cell. This production amount is higher than that of plants, specifically, more than 10 times per unit area of palm, which is said to be high in the production amount of lipids, carbohydrates, etc. It is an effective means.

そこで、光合成微生物を人工的に培養するための培養方法として、例えば特許文献1には、レースウェイポンドを用いる方法が記載されている。   Thus, as a culture method for artificially culturing photosynthetic microorganisms, for example, Patent Document 1 describes a method using a raceway pond.

特許第3524835号公報Japanese Patent No. 3524835

通常、レースウェイポンド型培養槽においては、レースウェイポンド型培養槽を構成する培養路を流れる培養液の攪拌手段として、パドル等が用いられる。しかしながら、光合成微生物を培養するレースウェイポンド型培養槽の場合、その水深は約10cm〜30cm程度と、通常の場合よりも浅いものとなる。その理由としては、培養路の底部に存在する光合成微生物に対しても太陽光を満遍なく照射させるためである。そして、このように十分な太陽光を全ての光合成微生物に照射することにより、十分な光合成を行わせることが可能となる。   Usually, in a race way pond type culture tank, a paddle or the like is used as a stirring means for a culture solution flowing through a culture path constituting the race way pond type culture tank. However, in the case of a raceway pond culture tank for culturing photosynthetic microorganisms, the water depth is about 10 cm to 30 cm, which is shallower than usual. The reason for this is to uniformly irradiate sunlight to the photosynthetic microorganisms present at the bottom of the culture path. And it becomes possible to perform sufficient photosynthesis by irradiating all the photosynthesis microorganisms with sufficient sunlight in this way.

しかしながら、このような浅い水深を有する培養液においては、二酸化炭素の曝気水深を確保することができない。従って、このような浅い水深である培養液に対して十分な二酸化炭素を溶解させるために過剰量の二酸化炭素を培養液に接触させなければならず、培養コストが増加するという課題がある   However, in a culture solution having such a shallow water depth, it is not possible to ensure the aeration depth of carbon dioxide. Therefore, in order to dissolve sufficient carbon dioxide in the culture solution having such a shallow water depth, an excessive amount of carbon dioxide must be brought into contact with the culture solution, resulting in an increase in culture cost.

本発明は上記の課題を解決すべくなされたものであり、その目的は、培養コストが抑制でき、浅い水深においても十分量の二酸化炭素を培養液に溶解可能なレースウェイポンド型培養槽を提供することである。   The present invention has been made to solve the above-mentioned problems, and an object of the present invention is to provide a raceway pond type culture tank capable of suppressing culture costs and dissolving a sufficient amount of carbon dioxide in a culture solution even at a shallow water depth. It is to be.

本発明者らは上記課題を解決すべく鋭意検討した結果、レースウェイポンド型培養槽において、攪拌手段上部にカバーを備え、当該カバー内部に二酸化炭素を供給することにより、培養コストが抑制でき、浅い水深においても十分量の二酸化炭素を培養液に溶解可能なレースウェイポンド型培養槽を提供することができることを見出し、本発明を完成させた。   As a result of intensive studies to solve the above problems, the present inventors have a cover on the stirring means in the raceway pond culture tank, and by supplying carbon dioxide inside the cover, the culture cost can be suppressed, The present inventors have found that a raceway pond type culture tank capable of dissolving a sufficient amount of carbon dioxide in a culture solution even at a shallow water depth can be provided.

即ち、本発明の要旨は、培養液が流れる培養路において攪拌手段が設けられ、当該攪拌手段により培養液の攪拌を行うレースウェイポンド型培養槽であって、当該攪拌手段の上方に、かつ、当該攪拌手段及び当該培養液の液面を覆うカバーが設けられ、当該カバーにより形成された空間に二酸化炭素が供給され、当該二酸化炭素が当該攪拌手段により当該培養液に溶解されることを特徴とする、レースウェイポンド型培養槽に関する。   That is, the gist of the present invention is a raceway pond type culture tank in which a stirring means is provided in a culture path through which a culture solution flows, and the culture solution is stirred by the stirring means, above the stirring means, and A cover that covers the liquid surface of the stirring means and the culture solution is provided, carbon dioxide is supplied to a space formed by the cover, and the carbon dioxide is dissolved in the culture solution by the stirring means. The present invention relates to a raceway pond type culture tank.

本発明によれば、培養コストが抑制でき、浅い水深においても十分量の二酸化炭素を培養液に溶解可能なレースウェイポンド型培養槽を提供することができる。   ADVANTAGE OF THE INVENTION According to this invention, the culture cost can be suppressed and the raceway pond type culture tank which can melt | dissolve a sufficient amount of carbon dioxide in a culture solution can be provided even in shallow water depth.

第一実施形態に係るレースウェイポンド型培養槽を模式的に表す斜視図である。It is a perspective view showing typically the race way pond type culture tank concerning a first embodiment. 第一実施形態に係るレースウェイポンド型培養槽に適用可能な攪拌手段の一具体例を模式的に表す図である。It is a figure which represents typically a specific example of the stirring means applicable to the raceway pond type culture tank which concerns on 1st embodiment. 第一実施形態に係るレースウェイポンド型培養槽において、図2に示す攪拌手段を用い、カバーを取り外した様子を模式的に表す図である。In the raceway pond type culture tank concerning a first embodiment, it is a figure showing typically signs that a cover is removed using the stirring means shown in FIG. 図2に示す攪拌手段を用いたレースウェイポンド型培養槽において、攪拌手段近傍を模式的に表す横断面図である。FIG. 3 is a cross-sectional view schematically showing the vicinity of the stirring means in the raceway pond culture tank using the stirring means shown in FIG. 2. 図2に示す攪拌手段を用いたレースウェイポンド型培養槽において、攪拌手段近傍を模式的に表す斜視図である。FIG. 3 is a perspective view schematically showing the vicinity of the stirring means in the raceway pond culture tank using the stirring means shown in FIG. 2.

以下、本発明を実施するための形態を詳細に説明するが、本実施形態は以下の内容に限定されるものではなく、その要旨を逸脱しない範囲内で任意に変更して実施することができる。   DESCRIPTION OF EMBODIMENTS Hereinafter, embodiments for carrying out the present invention will be described in detail. However, the present embodiment is not limited to the following contents, and can be arbitrarily changed and implemented without departing from the gist thereof. .

<第一実施形態>
図1は、第一実施形態に係るレースウェイポンド型培養槽を模式的に表す斜視図である。図1に示すように、第一実施形態に係るレースウェイポンド型培養槽Rは、培養液Mが流れる培養路8において攪拌手段P(図1においては図示していない。具体的な構成は後述する。)が設けられ、攪拌手段Pにより培養液Mの攪拌を行うレースウェイポンド型培養槽であって、攪拌手段Pの上方に、かつ、攪拌手段P及び培養液Mの液面を覆うカバー1が設けられ、カバー1により形成された空間(即ち、カバー1内部)に二酸化炭素が供給され、二酸化炭素が攪拌手段Pにより培養液Mに溶解されるようになっている。なお、図1及び図3において、培養液M中に示している矢印は培養液Mの流れの向きを表し、支持材2の軸まわりに示している矢印は支持材2の回転方向を表している。
<First embodiment>
FIG. 1 is a perspective view schematically showing a raceway pond culture tank according to the first embodiment. As shown in FIG. 1, the raceway pond type culture tank R according to the first embodiment includes a stirring means P (not shown in FIG. 1) in the culture path 8 through which the culture solution M flows. A raceway pond type culture tank in which the culture medium M is agitated by the agitation means P, and a cover that covers the liquid level of the agitation means P and the culture medium M above the agitation means P. 1 is provided, carbon dioxide is supplied to a space formed by the cover 1 (that is, inside the cover 1), and the carbon dioxide is dissolved in the culture medium M by the stirring means P. 1 and 3, the arrow shown in the culture medium M represents the direction of the flow of the culture medium M, and the arrow shown around the axis of the support material 2 represents the rotation direction of the support material 2. Yes.

二酸化炭素の具体的な供給方法として、図1においては、カバー1の上面に、パイプ、管等の二酸化炭素供給路4が接続されている。二酸化炭素供給路4はガス供給量調整バルブ5を介して二酸化炭素供給源3と接続され、第一実施形態に係るレースウェイポンド型培養槽Rはこのような構成を有することにより、カバー1の内部に二酸化炭素を供給することができるようになっている。   As a specific method for supplying carbon dioxide, in FIG. 1, a carbon dioxide supply path 4 such as a pipe or a pipe is connected to the upper surface of the cover 1. The carbon dioxide supply path 4 is connected to the carbon dioxide supply source 3 via the gas supply amount adjustment valve 5, and the raceway pond culture tank R according to the first embodiment has such a configuration, so that the cover 1 Carbon dioxide can be supplied inside.

なお、第一実施形態に係るレースウェイポンド型培養槽Rにおいて培養可能なものは、本発明の効果を著しく損なわない限り特に制限されない。ただし、培養液Mに効率良く二酸化炭素が溶解されるという観点から、好ましくは二酸化炭素を利用して培養される生物、より好ましくは光合成生物、さらに好ましくは光合成微生物、よりさらに好ましくは藻類、特に好ましくはユーグレナである。   In addition, what can be cultured in the raceway pond type culture tank R according to the first embodiment is not particularly limited as long as the effects of the present invention are not significantly impaired. However, from the viewpoint that carbon dioxide is efficiently dissolved in the culture medium M, preferably an organism cultured using carbon dioxide, more preferably a photosynthetic organism, more preferably a photosynthetic microorganism, even more preferably an algae, especially Euglena is preferable.

ユーグレナは鞭毛虫の一群で、運動性のある藻類として有名なミドリムシを含む。大部分のユーグレナは葉緑体を有しており、光合成を行って独立栄養生活を行うが、捕食性のものや吸収栄養性のものもある。ユーグレナは動物学と植物学との双方に分類される属である。   Euglena is a group of flagellates, including Euglena, which is famous as a motile algae. Most Euglena has a chloroplast and photosynthesis and autotrophic life, but there are also predatory and absorption nutrients. Euglena is a genus classified into both zoology and botany.

培養液Mの組成は、培養される生物の種類に応じて決定すればよい。例えば、第一実施形態に係るレースウェイポンド型培養槽Rを用いてユーグレナを培養する場合、培養液Mとしては、例えば、窒素源、リン源、ミネラルなどの栄養塩類を添加した培養液、例えば、改変Cramer−Myers培地((NHHPO 1.0g/L、KHPO 1.0g/L、MgSO・7HO 0.2g/L、CaCl・2HO 0.02g/L、Fe(SO・7HO 3mg/L、MnCl・4HO 1.8mg/L、CoSO・7HO 1.5mg/L、ZnSO・7HO 0.4mg/L、NaMoO・2HO 0.2mg/L、CuSO・5HO 0.02g/L、チアミン塩酸塩(ビタミンB) 0.1mg/L、シアノコバラミン(ビタミンB12)、(pH3.5))を用いることができる。なお、(NHHPOは、(NHSOやNHaqに変換することも可能である。 The composition of the culture solution M may be determined according to the type of organism to be cultured. For example, when euglena is cultured using the raceway pond type culture tank R according to the first embodiment, the culture solution M includes, for example, a culture solution to which nutrient salts such as a nitrogen source, a phosphorus source, and minerals are added, for example, Modified Cramer-Myers medium ((NH 4 ) 2 HPO 4 1.0 g / L, KH 2 PO 4 1.0 g / L, MgSO 4 .7H 2 O 0.2 g / L, CaCl 2 · 2H 2 O 0. 02 g / L, Fe 2 (SO 2 ) 3 · 7H 2 O 3 mg / L, MnCl 2 · 4H 2 O 1.8 mg / L, CoSO 4 · 7H 2 O 1.5 mg / L, ZnSO 4 · 7H 2 O 0 .4mg / L, Na 2 MoO 4 · 2H 2 O 0.2mg / L, CuSO 4 · 5H 2 O 0.02g / L, thiamine hydrochloride (vitamin B 1) 0.1mg / L, cyanocobalamin (bi Min B 12), can be used (pH 3.5)). Note that (NH 4 ) 2 HPO 4 can also be converted to (NH 4 ) 2 SO 4 or NH 3 aq.

また、培養液MのpHは好ましくは2以上、また、その上限は、好ましくは6以下、より好ましくは4.5以下である。pHを酸性側にすることにより、光合成微生物は他の微生物よりも優勢に生育することができるため、コンタミネーションを抑制することができる。   Further, the pH of the culture medium M is preferably 2 or more, and the upper limit thereof is preferably 6 or less, more preferably 4.5 or less. By setting the pH to the acidic side, the photosynthetic microorganisms can grow more dominantly than other microorganisms, so that contamination can be suppressed.

カバー1は、攪拌手段P(具体的な構成、機能等については後述する。)の上方に、かつ、攪拌手段P及び培養液Mの液面を覆うように設けられているものである。カバー1の大きさは特に制限されないが、攪拌手段Pを完全に覆うことができるものとする。カバー1の下部が培養液Mの液面に接触した場合、カバー1が培養液Mの流れを受けることになる、換言するとカバー1が培養液Mの流れを阻害することになるが、本発明の効果を著しく損なわない程度の阻害であれば、このような接触がなされていてもよい。また、カバー1が攪拌手段Pと比べて大きすぎる場合(即ち、カバー1を設けることにより形成される空間が大きすぎる場合)、培養液Mに溶解されない二酸化炭素の量が過剰なものとなる可能性がある。   The cover 1 is provided above the stirring means P (the specific configuration, functions, etc. will be described later) and so as to cover the liquid surface of the stirring means P and the culture medium M. The size of the cover 1 is not particularly limited, but it is assumed that the stirring means P can be completely covered. When the lower part of the cover 1 comes into contact with the liquid level of the culture solution M, the cover 1 receives the flow of the culture solution M, in other words, the cover 1 inhibits the flow of the culture solution M. Such contact may be made as long as the effect is not significantly impaired. Further, when the cover 1 is too large compared to the stirring means P (that is, when the space formed by providing the cover 1 is too large), the amount of carbon dioxide that is not dissolved in the culture medium M may be excessive. There is sex.

カバー1の形状も特に制限されない。図1に示す第一実施形態においては四角形状(即ち、半正八角形状)としているが、半円柱形状等であってもよい。   The shape of the cover 1 is not particularly limited. In the first embodiment shown in FIG. 1, a quadrangular shape (that is, a half regular octagon shape) is used, but a semi-cylindrical shape or the like may be used.

また、カバー1を構成する材料も特に制限されない。カバー1を構成する材料としては、例えば樹脂、金属等が挙げられ、これらは1種を単独で用いてもよく、2種以上を任意に組み合わせて用いてもよい。ただし、カバー1内部において培養液Mが攪拌され、培養液Mのミスト(飛沫)が生じる可能性が極めて高いことから、カバー1を構成する材料としては、培養液Mによって腐食されないものを用いることが特に好ましい。   Further, the material constituting the cover 1 is not particularly limited. Examples of the material constituting the cover 1 include resins and metals, and these may be used alone or in any combination of two or more. However, since the culture medium M is stirred inside the cover 1 and mist (spray) of the culture medium M is very likely to be generated, a material that does not corrode by the culture medium M should be used as the material constituting the cover 1. Is particularly preferred.

支持材2は後述する攪拌手段Pにおける平行方向攪拌手段6及び垂直方向攪拌手段7とが固定されるものである。支持材2を構成する材料等は特に制限されないが、カバー1と同様に培養液Mによって腐食されず、かつ、平行方向攪拌手段6及び垂直方向攪拌手段7を確実に固定できるものを用いることが好ましい。   The support member 2 is fixed with a parallel stirring means 6 and a vertical stirring means 7 in the stirring means P described later. The material constituting the support material 2 is not particularly limited. However, as in the case of the cover 1, a material that is not corroded by the culture medium M and that can securely fix the parallel stirring means 6 and the vertical stirring means 7 should be used. preferable.

また、支持材2の長さも特に制限されない。ただし、支持材2は、通常はギア、ベルト等を介して駆動手段(図示しない。)と接続される構成となっており、当該駆動手段によって支持材2を回転(即ち、攪拌手段Pを駆動)させることができるようになっている。そのため、ギアやベルトと接触させることができる程度に、支持材2が培養路8の外部に突き出す長さを有していることが好ましい。また、支持材2の太さも特に制限されず、平行方向攪拌手段6及び垂直方向攪拌手段7を確実に固定できる程度の強度を有する太さにすればよい。   Further, the length of the support member 2 is not particularly limited. However, the support member 2 is usually configured to be connected to drive means (not shown) via a gear, a belt, etc., and the support member 2 is rotated by the drive means (that is, the stirring means P is driven). ). Therefore, it is preferable that the support material 2 has a length that protrudes outside the culture path 8 to such an extent that it can be brought into contact with a gear or a belt. Further, the thickness of the support member 2 is not particularly limited, and may be a thickness having such a strength that the parallel stirring unit 6 and the vertical stirring unit 7 can be reliably fixed.

二酸化炭素供給源3は、カバー1内部に二酸化炭素を供給するものである。図1に示す第一実施形態においては、二酸化炭素が充填されたガスボンベを用いている。ただし、二酸化炭素供給源3はガスボンベに何ら限定されるものではなく、例えば二酸化炭素を含む工場等からの排気ガスを用いてもよい。ただし、工場等からの排気ガスを二酸化炭素供給源3として用いる場合には、予め培養する例えば光合成微生物の成長及び培養を阻害する成分を除去したガスを用いることが好ましい。このような成分を除去する装置(例えば硫黄酸化物(SO)や窒素酸化物(NO)の除去装置等)は公知の任意のものを用いることができ、その設置場所も任意である。例えば、予め当該装置により除去したガスを二酸化炭素供給路4(後述する。)に流通させてもよいし、当該装置をカバー1の上部に設け、除去前の排気ガスを二酸化炭素供給路4に流通させて当該装置で当該成分を除去後、二酸化炭素をカバー1内部に供給する構成としてもよい。なお、培養する光合成微生物が例えば硫黄酸化物や窒素酸化物等に対して耐性があったり、浄化作用があったりするものであれば、硫黄酸化物や窒素酸化物等の除去は行わなくてもよい。 The carbon dioxide supply source 3 is for supplying carbon dioxide into the cover 1. In the first embodiment shown in FIG. 1, a gas cylinder filled with carbon dioxide is used. However, the carbon dioxide supply source 3 is not limited to a gas cylinder, and for example, exhaust gas from a factory containing carbon dioxide may be used. However, when exhaust gas from a factory or the like is used as the carbon dioxide supply source 3, it is preferable to use a gas that has been previously cultured, for example, from which a component that inhibits the growth and cultivation of photosynthetic microorganisms has been removed. As a device for removing such components (for example, a sulfur oxide (SO x ) or nitrogen oxide (NO x ) removal device), any known device can be used, and the installation location thereof is also arbitrary. For example, the gas previously removed by the apparatus may be circulated through the carbon dioxide supply path 4 (described later), or the apparatus is provided in the upper part of the cover 1, and the exhaust gas before removal is supplied to the carbon dioxide supply path 4. It is good also as a structure which supplies carbon dioxide inside the cover 1 after distribute | circulating and removing the said component with the said apparatus. If the photosynthetic microorganisms to be cultured are resistant to, for example, sulfur oxides or nitrogen oxides or have a purifying action, sulfur oxides or nitrogen oxides need not be removed. Good.

二酸化炭素供給路4は、カバー1内部と二酸化炭素供給源3とを接続するものである。二酸化炭素供給路4の長さや構成材料は特に制限されないが、供給される二酸化炭素の損失をできるだけ抑制する観点から、可能な限り短いことが好ましい。また、構成材料は二酸化炭素によって劣化しないものが好ましく、特に、工場等からの排気ガスを二酸化炭素供給源3として用い、例えばSOやNOの除去装置をカバー1の上部に設ける場合、当該排気ガスに含まれる二酸化炭素以外の成分によっても劣化しない材料を用いることが好ましい。 The carbon dioxide supply path 4 connects the inside of the cover 1 and the carbon dioxide supply source 3. The length and constituent materials of the carbon dioxide supply path 4 are not particularly limited, but are preferably as short as possible from the viewpoint of suppressing loss of supplied carbon dioxide as much as possible. Further, it is preferable that the constituent material is not deteriorated by carbon dioxide. In particular, when exhaust gas from a factory or the like is used as the carbon dioxide supply source 3, for example, when an SO x or NO x removal device is provided on the top of the cover 1, It is preferable to use a material that does not deteriorate due to components other than carbon dioxide contained in the exhaust gas.

二酸化炭素供給路4の途中には、二酸化炭素供給路4を流れるガスの流量を調整するガス供給量調整バルブ5が設けられ、これにより、カバー1内部に供給される二酸化炭素の量を調整することができる。ガス供給量調整バルブ5は手動で操作されてもよく、自動で操作されてもよい。   A gas supply amount adjustment valve 5 that adjusts the flow rate of the gas flowing through the carbon dioxide supply passage 4 is provided in the middle of the carbon dioxide supply passage 4, thereby adjusting the amount of carbon dioxide supplied into the cover 1. be able to. The gas supply amount adjusting valve 5 may be manually operated or automatically operated.

なお、供給する二酸化炭素の量は必ずしも一定である必要は無く、例えば時間や天候等に応じてその供給量を変動させるようにすることができる。具体的には、培養する光合成微生物の種類(活動状況)に応じて供給量を変動させてもよい。例えば、午前に光合成が活発であるならば午前に二酸化炭素の量を多く、午後に光合成が活発であるならば午後に二酸化炭素の量を多く、夜間に光合成が行われないならば夜間は二酸化炭素の供給を停止(培養槽全体の運転を停止)する等して、光合成微生物の活動状況に応じて二酸化炭素を供給する。このようにすることで、二酸化炭素の無駄な消費を抑制すること、換言すると、光合成微生物に取り込まれなかった二酸化炭素が大気中に無駄に放散されることを防止できる。ちなみに、第一実施形態に係るレースウェイポンド型培養槽Rは、このような光合成微生物の活動状況に応じて二酸化炭素を供給する機能を備えているものとする。
このような供給量の変動は手動でガス供給量調整バルブ5を操作して行ってもよいし、例えば上記の変動を行わせることができるプログラムが記録された装置(例えば時間に応じて供給量を変動させる場合にはタイマー等)とガス供給量調整バルブ5とを連動させて、上記の操作を自動で行わせてもよい。
Note that the amount of carbon dioxide to be supplied is not necessarily constant. For example, the supply amount can be varied according to time, weather, or the like. Specifically, the supply amount may be varied according to the type (activity status) of photosynthetic microorganisms to be cultured. For example, if photosynthesis is active in the morning, the amount of carbon dioxide is increased in the morning, if photosynthesis is active in the afternoon, the amount of carbon dioxide is increased in the afternoon, and if photosynthesis is not performed at night, the amount of carbon dioxide is increased at night. Carbon dioxide is supplied according to the activity status of photosynthetic microorganisms, for example, by stopping the supply of carbon (stopping the operation of the entire culture tank). By doing so, it is possible to suppress wasteful consumption of carbon dioxide, in other words, it is possible to prevent wasteful release of carbon dioxide that has not been taken into the photosynthetic microorganism. Incidentally, the raceway pond culture tank R according to the first embodiment is assumed to have a function of supplying carbon dioxide according to the activity state of such photosynthetic microorganisms.
Such a change in the supply amount may be performed manually by operating the gas supply amount adjustment valve 5, or for example, a device in which a program capable of performing the above-described change is recorded (for example, a supply amount according to time). When changing the above, the above operation may be automatically performed by interlocking the gas supply amount adjusting valve 5 with a timer or the like.

さらに、培養する光合成微生物の種類(活動パターン)に応じて供給量を変動させてもよい。例えば、培養する光合成微生物の活動が午後に特に活発になる場合、通常は光合成も午後に活発に行われるため、午後において二酸化炭素の供給量を増加させる等してもよい。   Further, the supply amount may be varied according to the type (activity pattern) of photosynthetic microorganisms to be cultured. For example, when the activity of the photosynthetic microorganisms to be cultured becomes particularly active in the afternoon, normally, photosynthesis is also actively performed in the afternoon. Therefore, the supply amount of carbon dioxide may be increased in the afternoon.

カバー1内部に供給される二酸化炭素の量は特に制限されない。ただし、培養コストを抑制しつつ、かつ、効率良く培養液Mに二酸化炭素を溶解させる観点から、培養液Mの二酸化炭素の溶解量が培養液Mの飽和二酸化炭素濃度となるように、カバー1内部に二酸化炭素を供給することが好ましい。   The amount of carbon dioxide supplied into the cover 1 is not particularly limited. However, from the viewpoint of efficiently dissolving carbon dioxide in the culture medium M while suppressing the culture cost, the cover 1 is set so that the amount of carbon dioxide dissolved in the culture medium M becomes the saturated carbon dioxide concentration of the culture medium M. It is preferable to supply carbon dioxide inside.

このような適切な量の二酸化炭素をカバー1内部に供給するために、例えばカバー1内部に二酸化炭素濃度測定器、培養液Mの溶存二酸化炭素濃度測定器等を設け、カバー1内部及び培養液M中の二酸化炭素濃度を測定することが好ましい。そして、測定値に基づいて二酸化炭素の供給量を変動させることにより、より適切な量の二酸化炭素をカバー1内部に供給することができる。   In order to supply such an appropriate amount of carbon dioxide to the inside of the cover 1, for example, a carbon dioxide concentration measuring device, a dissolved carbon dioxide concentration measuring device for the culture solution M, etc. are provided inside the cover 1, and the inside of the cover 1 and the culture solution are provided. It is preferable to measure the carbon dioxide concentration in M. A more appropriate amount of carbon dioxide can be supplied to the inside of the cover 1 by changing the supply amount of carbon dioxide based on the measured value.

また、培養液Mの流れを阻害しない程度にカバー1を培養液Mに接触させ、カバー1内部の圧力及び二酸化炭素濃度を測定しながら培養を行ってもよい。このようにすることで、例えば二酸化炭素が不足してカバー1内の圧力が下がったら二酸化炭素を供給し、カバー1内部の圧力が所定のものになったら、供給を止める制御を行うことができ、過剰な二酸化炭素量(即ち、外部に放散される二酸化炭素量)をより確実に減少させることができる。   In addition, the cover 1 may be brought into contact with the culture medium M so as not to inhibit the flow of the culture medium M, and the culture may be performed while measuring the pressure and carbon dioxide concentration inside the cover 1. In this way, for example, when carbon dioxide is insufficient and the pressure in the cover 1 drops, carbon dioxide is supplied, and when the pressure inside the cover 1 reaches a predetermined value, the supply can be stopped. In addition, the amount of excess carbon dioxide (that is, the amount of carbon dioxide released to the outside) can be reduced more reliably.

さらには、上記のように、本発明の効果を著しく損なわない程度にカバー1を培養液Mに接触させ、カバー1内部に二酸化炭素を供給して培養液Mに二酸化炭素を溶解させる際、培養液Mから放散される二酸化炭素量も考慮して、カバー1内部に供給する二酸化炭素量を制御してもよい。   Furthermore, as described above, when the cover 1 is brought into contact with the culture medium M to such an extent that the effects of the present invention are not significantly impaired, carbon dioxide is supplied into the cover 1 to dissolve the carbon dioxide in the culture medium M. In consideration of the amount of carbon dioxide released from the liquid M, the amount of carbon dioxide supplied into the cover 1 may be controlled.

次に、第一実施形態に係るレースウェイポンド型培養槽Rに適用可能な攪拌手段Pの一具体例を、図2を参照しながら説明する。   Next, a specific example of the stirring means P applicable to the raceway pond culture tank R according to the first embodiment will be described with reference to FIG.

図2は、第一実施形態に係るレースウェイ培養装置Rに適用可能な攪拌手段Pの一具体例(以下、適宜、「第一実施形態に係る攪拌手段」と言う。)を模式的に表す図である。図2に示す第一実施形態に係る攪拌手段Pは、培養液Mの流れ方向に対して平行(即ち、同方向)若しくは略平行な方向の攪拌を行う平行方向攪拌手段6と、流れ方向に対して垂直若しくは略垂直な方向の攪拌を行う垂直方向攪拌手段7と、を備えている。そして、平行方向攪拌手段6及び垂直方向攪拌手段7は、いずれも等間隔となるように支持材2に固定されている。また、第一実施形態に係る攪拌手段Pは、培養液Mを攪拌する機能に加えて、培養液Mの流れを作り出す機能(即ち、培養液Mの循環手段としての機能)も有している。   FIG. 2 schematically shows a specific example of the stirring means P applicable to the raceway culture apparatus R according to the first embodiment (hereinafter referred to as “stirring means according to the first embodiment” as appropriate). FIG. The stirring means P according to the first embodiment shown in FIG. 2 includes a parallel direction stirring means 6 that performs stirring in a direction parallel (that is, the same direction) or substantially parallel to the flow direction of the culture solution M, and a flow direction. And a vertical stirring means 7 for stirring in a vertical or substantially vertical direction. And the parallel direction stirring means 6 and the vertical direction stirring means 7 are being fixed to the support material 2 so that all may become equal intervals. In addition to the function of stirring the culture medium M, the stirring unit P according to the first embodiment also has a function of creating a flow of the culture medium M (that is, a function as a circulation unit of the culture medium M). .

図2に示すように、第一実施形態に係る攪拌手段Pにおいては、平行方向攪拌手段6として板状羽根を、垂直方向攪拌手段7として櫛状羽根を用いている。また、支持材2に固定される、平行方向攪拌手段6及び垂直方向攪拌手段7の数は図2においてはいずれも4つずつとしているが、固定される各手段の数は4つに限定されるものではない。また、平行方向攪拌手段6及び垂直方向攪拌手段7の数を異なるものにしてもよい。さらに、図2においては、平行方向攪拌手段6及び垂直方向攪拌手段7が交互に支持材2に設けられているが、必ずしも交互に設けられる必要は無い。また、垂直方向攪拌手段7を構成する櫛部7a(後述する。)の数も特に制限されない。   As shown in FIG. 2, in the stirring means P according to the first embodiment, plate-shaped blades are used as the parallel direction stirring means 6, and comb-shaped blades are used as the vertical direction stirring means 7. Further, the number of the parallel direction stirring means 6 and the vertical direction stirring means 7 fixed to the support member 2 is four in FIG. 2, but the number of each fixed means is limited to four. It is not something. Further, the numbers of the parallel stirring means 6 and the vertical stirring means 7 may be different. Further, in FIG. 2, the parallel direction stirring means 6 and the vertical direction stirring means 7 are alternately provided on the support member 2, but are not necessarily provided alternately. Further, the number of comb portions 7a (described later) constituting the vertical direction stirring means 7 is not particularly limited.

また、図2に示す平行方向攪拌手段6は、その端部(支持材2に固定されていない側)が折り曲げられて形成されている。図2に示すように、平行方向攪拌手段6の端部が折り曲げられた形状を有する板状羽根とし、さらに折り曲げ角を鈍角とすることにより、カバー1内部の二酸化炭素を培養液M中により効率良く溶解させることができる。   Moreover, the parallel direction stirring means 6 shown in FIG. 2 is formed by bending its end (the side not fixed to the support member 2). As shown in FIG. 2, the plate-like blade having a shape in which the end of the parallel stirring means 6 is bent, and the bending angle is an obtuse angle, so that carbon dioxide inside the cover 1 is more efficiently contained in the culture medium M. Can be dissolved well.

平行方向攪拌手段6の大きさ及び厚さ、並びに垂直方向攪拌手段7の長さ及び太さ等は特に制限されず、平行方向攪拌手段6及び垂直方向攪拌手段7が、培養路8の底部に接触しない程度の大きさ及び長さにし、各手段の強度が保てる程度の厚さ及び太さにすればよい。   The size and thickness of the parallel stirring means 6 and the length and thickness of the vertical stirring means 7 are not particularly limited, and the parallel stirring means 6 and the vertical stirring means 7 are located at the bottom of the culture path 8. The size and length should not be in contact with each other, and the thickness and thickness should be sufficient to maintain the strength of each means.

図3は、第一実施形態に係るレースウェイポンド型培養槽Rにおいて攪拌手段Pとして図2に示す攪拌手段を用い、カバー1を取り外した様子を模式的に表す図である。図3に示すように、攪拌手段Pが駆動することにより、培養路8内で浅い水深の培養液Mに対して、培養コストを抑制しつつも十分量の二酸化炭素を溶解させることができる。また、培養液Mの流れを作り出すこともできる。   FIG. 3 is a diagram schematically showing a state in which the cover 1 is removed using the stirring means shown in FIG. 2 as the stirring means P in the raceway pond culture tank R according to the first embodiment. As shown in FIG. 3, by driving the stirring means P, a sufficient amount of carbon dioxide can be dissolved in the culture medium M having a shallow water depth in the culture channel 8 while suppressing the culture cost. Moreover, the flow of the culture solution M can also be created.

ここで、図2に示す攪拌手段Pを構成する、平行方向攪拌手段6及び垂直方向攪拌手段7について、図4及び図5を参照しながら説明する。図4は、図2に示す攪拌手段Pを用いたレースウェイポンド型培養槽Rにおいて、攪拌手段P近傍を模式的に表す横断面図、図5は、図2に示す攪拌手段Pを用いたレースウェイポンド型培養槽Rにおいて、攪拌手段P近傍を模式的に表す斜視図である。なお、図5においては、説明の簡略化のために、垂直方向攪拌手段7を構成する一部の櫛部7aのみを示している。   Here, the parallel direction stirring means 6 and the vertical direction stirring means 7 constituting the stirring means P shown in FIG. 2 will be described with reference to FIGS. 4 is a cross-sectional view schematically showing the vicinity of the stirring means P in the raceway pond culture tank R using the stirring means P shown in FIG. 2, and FIG. 5 uses the stirring means P shown in FIG. FIG. 3 is a perspective view schematically showing the vicinity of a stirring means P in a raceway pond culture tank R. In FIG. 5, only a part of the comb portions 7 a constituting the vertical stirring means 7 is shown for the sake of simplicity of explanation.

なお、図4において、白抜きの矢印がカバー1内部における二酸化炭素の流れる向きを表し、培養液M中に示されている円形状のものが、培養液M中に溶解している二酸化炭素を表している。また、黒い実線矢印は各手段(及び支持材2)が回転する方向を表している。   In FIG. 4, the white arrow indicates the direction in which carbon dioxide flows in the cover 1, and the circular shape shown in the culture solution M indicates the carbon dioxide dissolved in the culture solution M. Represents. Moreover, the black solid line arrow represents the direction in which each means (and support material 2) rotates.

図4に示すように、カバー1内部に供給された二酸化炭素はカバー1内部に一様に拡散するが、平行方向攪拌手段6の回転により二酸化炭素の流れる向きがほぼ一方向となり(図4において、支持材2を中心として反時計回り方向。)、拡散した二酸化炭素の大部分が、平行方向攪拌手段6とともに培養液Mに強制的に接触させられる(即ち、二酸化炭素が培養液Mに押し込められる)ことになる。そして、平行方向攪拌手段6により培養液Mに強制的に接触させられた二酸化炭素は培養液Mに溶解し、溶解し切れなかった二酸化炭素は培養液Mとカバー1との間に存在する隙間を通って外部に放出される。
なお、図4に示す実施形態においては、カバー1の端部と培養液Mとは接触していないが、上記のようにカバー1の端部と培養液Mとを接触させて、二酸化炭素を外部に放出させない構成としてもよい。
As shown in FIG. 4, the carbon dioxide supplied into the cover 1 is uniformly diffused into the cover 1, but the direction of carbon dioxide flow is almost unidirectional by the rotation of the parallel stirring means 6 (in FIG. 4). The counter-clockwise direction with respect to the support material 2), most of the diffused carbon dioxide is forcibly brought into contact with the culture medium M together with the parallel stirring means 6 (that is, carbon dioxide is pushed into the culture medium M). Will be). The carbon dioxide forced to come into contact with the culture medium M by the parallel stirring means 6 is dissolved in the culture medium M, and the carbon dioxide that has not been completely dissolved exists between the culture medium M and the cover 1. It is discharged to the outside through.
In the embodiment shown in FIG. 4, the end of the cover 1 and the culture medium M are not in contact with each other. However, as described above, the end of the cover 1 and the culture medium M are brought into contact with each other, thereby carbon dioxide. It is good also as a structure which does not discharge | release outside.

また、平行方向攪拌手段6及び垂直方向攪拌手段7によって培養液Mの攪拌が繰り返され、カバー1内部には培養液Mのミスト(しぶき)が飛び散っている状態であり、二酸化炭素で充満した空間に当該飛沫が曝露されていることになる。従って、当該飛沫は、周辺の雰囲気である二酸化炭素を溶解しやすく、このようにして二酸化炭素を溶解した飛沫は、再び培養液Mとなって培養路8を流れることになる。   Further, the stirring of the culture medium M is repeated by the parallel stirring means 6 and the vertical stirring means 7, and the mist (splash) of the culture liquid M is scattered inside the cover 1 and is a space filled with carbon dioxide. The droplets are exposed to. Therefore, the droplets easily dissolve carbon dioxide, which is the surrounding atmosphere, and the droplets in which carbon dioxide has been dissolved in this way again become the culture solution M and flow through the culture path 8.

以上のように、攪拌手段Pの上方に、かつ、攪拌手段P及び培養液Mの液面を覆うカバー1が設けられ、カバー1内部に二酸化炭素が供給されることにより、効率よく培養液Mに二酸化炭素を溶解させることができる。なお、攪拌手段Pが平行方向攪拌手段6又は垂直方向攪拌手段7を有さず、攪拌手段Pが例えばスクリュー等であっても、例えば水流によって生じる気流の乱れや発生する飛沫等によって、上記の効果と同様の効果を奏する。   As described above, the cover 1 is provided above the stirring means P and covers the liquid surfaces of the stirring means P and the culture medium M, and carbon dioxide is supplied into the cover 1, so that the culture medium M can be efficiently used. It is possible to dissolve carbon dioxide. Even if the stirring means P does not have the parallel direction stirring means 6 or the vertical direction stirring means 7 and the stirring means P is, for example, a screw or the like, for example, due to the turbulence of the air flow caused by the water flow or the generated droplets, the above-mentioned Has the same effect as the effect.

また、第一実施形態に係るレースウェイポンド型培養槽Rにおいては、攪拌手段として図2に示す攪拌手段Pを用いているため、以下のような利点も得られる。   Moreover, in the raceway pond type culture tank R according to the first embodiment, since the stirring means P shown in FIG. 2 is used as the stirring means, the following advantages are also obtained.

レースウェイポンド型培養槽を用いて光合成微生物を培養する場合、通常は培養液の水深が浅いため培養液の移動が二次元方向(即ち、水深方向に垂直な方向)のみになる傾向があり、水深方向の培養液の移動が起き難いという課題がある。従って、培養路の底部に存在する光合成微生物まで太陽光が到達しうる程度の浅さに培養液の水深を調整しても依然としてその培養条件が不十分である可能性が高い。そのため、十分に太陽光が照射される培養液液面付近に存在する光合成微生物層と、太陽光が照射されにくい培養路底部に存在する光合成微生物層と、の二層に分かれやすくなり、上層(液面に近い層)と下層(培養路の底部に近い層)との混合が行われにくく、一様な培養が行われにくいという課題がある。   When cultivating photosynthetic microorganisms using a raceway pond culture tank, the culture medium tends to move only in a two-dimensional direction (ie, a direction perpendicular to the water depth direction) because the culture medium is usually shallow. There is a problem that it is difficult for the culture medium to move in the depth direction. Therefore, even if the depth of the culture solution is adjusted to such a shallow depth that sunlight can reach the photosynthetic microorganisms present at the bottom of the culture path, the culture conditions are still likely to be insufficient. Therefore, it becomes easy to separate into two layers, a photosynthetic microorganism layer that exists near the liquid surface of the culture solution that is sufficiently irradiated with sunlight, and a photosynthetic microorganism layer that exists at the bottom of the culture path where sunlight is difficult to be irradiated. There is a problem that mixing of the layer close to the liquid surface) and the lower layer (layer close to the bottom of the culture path) is difficult to perform, and uniform culture is difficult to be performed.

しかしながら、第一実施形態に係る攪拌手段Pを用いることにより、平行方向攪拌手段6は、上記の二酸化炭素溶解の作用に加えて、培養液Mの流れを形成する作用も有する。さらに、培養路8の底部から培養液Mを掻き揚げる(即ち、底部から培養液Mを掬い上げる)ように平行方向攪拌手段6が駆動するため、培養路8における培養液Mの上下方向(図4における紙面上下方向)の攪拌を行うこともできる。   However, by using the stirring means P according to the first embodiment, the parallel direction stirring means 6 has an action of forming the flow of the culture medium M in addition to the action of dissolving carbon dioxide. Furthermore, since the parallel stirring means 6 is driven so as to scoop up the culture medium M from the bottom of the culture path 8 (that is, scoop up the culture medium M from the bottom), the vertical direction of the culture medium M in the culture path 8 (FIG. 4 in the vertical direction of the paper).

さらに、図5に示すように、垂直方向攪拌手段7が駆動することにより、垂直方向攪拌手段7を構成する櫛部7aの移動後には、図5において黒い細実線矢印で示すように横方向(即ち、培養液Mの流れ方向に対して垂直若しくは略垂直。厳密には垂直ではないが、説明を簡略化するために「垂直」と呼称している。)の培養液Mの流れが生じる。従って、第一実施形態に係る攪拌手段Pを用いることにより、平行方向攪拌手段6による培養液Mの上下方向の攪拌のみならず、培養液Mの流れ方向に対して垂直な方向の攪拌を行うこともできる。   Further, as shown in FIG. 5, when the vertical stirring means 7 is driven, after the comb portion 7 a constituting the vertical stirring means 7 is moved, as shown by the black thin solid arrows in FIG. , Perpendicular to or substantially perpendicular to the flow direction of the culture medium M. Although not strictly perpendicular, it is referred to as “vertical” in order to simplify the description. Therefore, by using the stirring means P according to the first embodiment, not only the vertical stirring of the culture medium M by the parallel stirring means 6 but also the stirring in the direction perpendicular to the flow direction of the culture liquid M is performed. You can also

従って、第一実施形態に係るレースウェイポンド型培養槽Rにおいて攪拌手段として図2に示す攪拌手段Pを用いることにより、培養液Mの流れ方向に対して平行(即ち、流れ方向と同方向)及び垂直、さらには培養液Mの上下方向の攪拌を、一体的に形成された一つの攪拌手段によって行うことができ、上記の培養液Mに対する二酸化炭素溶解の促進に加え、均一な培養液Mの攪拌をも行うことが可能となる。   Therefore, by using the agitation means P shown in FIG. 2 as the agitation means in the raceway pond culture tank R according to the first embodiment, it is parallel to the flow direction of the culture medium M (that is, the same direction as the flow direction). In addition, the stirring of the culture medium M in the vertical direction and the vertical direction of the culture medium M can be performed by one integrally formed stirring means, and in addition to the promotion of carbon dioxide dissolution in the culture medium M, the uniform culture medium M It is also possible to perform stirring.

<まとめ>
以上、本実施形態に係るレースウェイポンド型培養槽Rを、攪拌手段として図2に示す攪拌手段Pを用いて具体的に説明したが、本実施形態に係るレースウェイポンド型培養槽Rに適用可能な攪拌手段は、図2に示す攪拌手段Pに限定されるものではない。即ち、本実施形態に係るレースウェイポンド型培養槽Rに設けられる攪拌手段としては、公知の任意の攪拌手段を用いることができる。また、レースウェイポンド型培養槽Rに設けられる攪拌手段の数も任意であり、設ける攪拌手段の数に応じてカバー1をそれぞれ設ければよい。
<Summary>
The raceway pond culture tank R according to the present embodiment has been specifically described using the stirring means P shown in FIG. 2 as the stirring means, but is applied to the raceway pond culture tank R according to the present embodiment. The possible stirring means is not limited to the stirring means P shown in FIG. That is, as the stirring means provided in the raceway pond culture tank R according to the present embodiment, any known stirring means can be used. The number of stirring means provided in the raceway pond culture tank R is also arbitrary, and the cover 1 may be provided according to the number of stirring means provided.

さらに、上記のように、第一実施形態に係る攪拌手段Pは培養液Mの流れを作り出す機能(即ち、培養液Mの循環手段としての機能)も有しているが、攪拌手段は循環手段としての機能を有する必要はなく、循環手段としての機能を有さない攪拌手段と、循環手段とを組み合わせて本実施形態に係るレースウェイポンド型培養槽Rとしてもよい。   Furthermore, as described above, the stirring means P according to the first embodiment also has a function of creating a flow of the culture solution M (that is, a function as a circulation means of the culture solution M). The raceway pond culture tank R according to the present embodiment may be combined with a stirring means that does not have a function as a circulation means and a circulation means.

また、図1に示す第一実施形態に係るレースウェイポンド型培養槽Rにおいては、二酸化炭素供給源3からカバー1内部に直接二酸化炭素が供給される構成としているが、例えば、カバー1外部において、別の溶解手段(図示しない。)によって二酸化炭素を培養液Mに溶解させ、当該二酸化炭素が溶解した培養液Mがカバー1の下部を通過する際にカバー1内部に二酸化炭素が放散されることにより、二酸化炭素がカバー1内部に供給される構成としてもよい。このような構成とすることにより、カバー1内部に放散した二酸化炭素を再び培養液Mに溶解させることができ、外部へ放散する二酸化炭素量を減少させることができ、ひいては培養コストを削減できる。   In the raceway pond culture tank R according to the first embodiment shown in FIG. 1, carbon dioxide is directly supplied from the carbon dioxide supply source 3 to the inside of the cover 1. Carbon dioxide is dissolved in the culture solution M by another dissolving means (not shown), and the carbon dioxide is diffused into the cover 1 when the culture solution M in which the carbon dioxide is dissolved passes through the lower part of the cover 1. Thus, the carbon dioxide may be supplied into the cover 1. By setting it as such a structure, the carbon dioxide diffused inside the cover 1 can be dissolved again in the culture solution M, the amount of carbon dioxide diffused outside can be reduced, and culture | cultivation cost can be reduced by extension.

その他にも、本発明の要旨を損なわない限り、上記の内容を適宜変更して実施可能であることは当業者にとって明らかである。   In addition, it will be apparent to those skilled in the art that the above-described content can be modified as appropriate without departing from the spirit of the present invention.

1 カバー
2 支持材
3 二酸化炭素供給源
4 二酸化炭素供給路
5 ガス供給量調整バルブ
6 平行方向攪拌手段
7 垂直方向攪拌手段
7a 櫛部
8 培養路
M 培養液
P 攪拌手段
R レースウェイポンド型培養槽
DESCRIPTION OF SYMBOLS 1 Cover 2 Support material 3 Carbon dioxide supply source 4 Carbon dioxide supply path 5 Gas supply amount adjustment valve 6 Parallel direction stirring means 7 Vertical direction stirring means 7a Comb portion 8 Culture path M Culture solution P Stirring means R Raceway pond type culture tank

Claims (3)

培養液が流れる培養路において攪拌手段が設けられ、該攪拌手段により培養液の攪拌を行うレースウェイポンド型培養槽であって、
該攪拌手段の上方に、かつ、該攪拌手段及び該培養液の液面を覆うカバーが設けられ、
該カバーにより形成された空間に二酸化炭素が供給され、該二酸化炭素が該攪拌手段により該培養液に溶解されるように構成されている
ことを特徴とする、レースウェイポンド型培養槽。
A raceway pond type culture tank provided with stirring means in the culture path through which the culture solution flows, and stirring the culture solution with the stirring means,
A cover is provided above the stirring means and covers the liquid level of the stirring means and the culture solution,
A raceway pond culture tank, wherein carbon dioxide is supplied to a space formed by the cover, and the carbon dioxide is dissolved in the culture solution by the stirring means.
該攪拌手段が、
該培養液の流れ方向に対して平行若しくは略平行な方向の攪拌を行う平行方向攪拌手段と、
該流れ方向に対して垂直若しくは略垂直な方向の攪拌を行う垂直方向攪拌手段と、
該平行方向攪拌手段及び該垂直方向攪拌手段を固定する支持材と、
を少なくとも備えている
ことを特徴とする、請求項1に記載のレースウェイポンド型培養槽。
The stirring means is
Parallel direction stirring means for stirring in a direction parallel or substantially parallel to the flow direction of the culture solution;
Vertical stirring means for stirring in a direction perpendicular or substantially perpendicular to the flow direction;
A support for fixing the parallel stirring means and the vertical stirring means;
The raceway pond culture tank according to claim 1, comprising at least
該平行方向攪拌手段が板状羽根であり、
該垂直方向攪拌手段が櫛状羽根である
ことを特徴とする、請求項1又は2に記載のレースウェイポンド型培養槽。
The parallel stirring means is a plate-shaped blade;
The raceway pond culture tank according to claim 1 or 2, wherein the vertical stirring means is a comb blade.
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CN107155333B (en) * 2014-07-08 2021-02-12 生物系统公司 Bioreactor for producing and harvesting microalgae
KR101936268B1 (en) * 2017-02-15 2019-01-08 재단법인 탄소순환형 차세대 바이오매스 생산전환 기술연구단 Mixing Apparatus in Microalgae Cultivating Reactor and Method for Cultivation of Microalgae using the same
CN107723214A (en) * 2017-11-13 2018-02-23 烟台大学 Plain type raceway pond and its application method for microdisk electrode
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KR102026721B1 (en) * 2017-12-27 2019-10-04 주식회사 에이이 Apparatus Capable of Controlling Light Soure and temperature for Outdoor Mass Culturing of Microalgae
CN110422930A (en) * 2019-07-31 2019-11-08 宁波倍加福生物技术有限公司 Racetrack biodegrade pond and its working method
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