CN107365689A - A kind of staged pesticide degradation bacteria and net dirty carrier loop flowing load device - Google Patents
A kind of staged pesticide degradation bacteria and net dirty carrier loop flowing load device Download PDFInfo
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- CN107365689A CN107365689A CN201710571499.5A CN201710571499A CN107365689A CN 107365689 A CN107365689 A CN 107365689A CN 201710571499 A CN201710571499 A CN 201710571499A CN 107365689 A CN107365689 A CN 107365689A
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- 241000894006 Bacteria Species 0.000 title claims abstract description 127
- 230000015556 catabolic process Effects 0.000 title claims abstract description 80
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 80
- 239000000575 pesticide Substances 0.000 title claims abstract description 49
- 238000001179 sorption measurement Methods 0.000 claims abstract description 80
- 239000007788 liquid Substances 0.000 claims abstract description 32
- 239000002699 waste material Substances 0.000 claims abstract description 5
- 230000002745 absorbent Effects 0.000 claims description 20
- 239000002250 absorbent Substances 0.000 claims description 20
- 238000003756 stirring Methods 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 6
- 238000004064 recycling Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 description 14
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 11
- 230000000593 degrading effect Effects 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 239000003905 agrochemical Substances 0.000 description 8
- 239000011449 brick Substances 0.000 description 8
- 239000003987 organophosphate pesticide Substances 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 7
- 239000005944 Chlorpyrifos Substances 0.000 description 4
- SBPBAQFWLVIOKP-UHFFFAOYSA-N chlorpyrifos Chemical compound CCOP(=S)(OCC)OC1=NC(Cl)=C(Cl)C=C1Cl SBPBAQFWLVIOKP-UHFFFAOYSA-N 0.000 description 4
- 230000015271 coagulation Effects 0.000 description 4
- 238000005345 coagulation Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000005484 gravity Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 241000228245 Aspergillus niger Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 229920005479 Lucite® Polymers 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000003575 carbonaceous material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000025218 regulation of catabolic process Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
Abstract
Proposed by the present invention is a kind of staged pesticide degradation bacteria and net dirty carrier loop flowing load device, and its structure includes adsorption box, discharge opeing case, catch box, flow speed control valve, staged fixed mount；Wherein, staged fixed mount has some steps, the staged fixed mount bottom has catch box, staged fixed mount top has discharge opeing case, there is adsorption box on each step among staged fixed mount, the bottom of discharge opeing case is connected with the side of adjacent adsorption box by connecting tube, and the bottom of each adsorption box is connected with the side of the adsorption box on next layer of step by connecting tube, and the bottom bottom of an adsorption box is connected with the side of catch box by connecting tube.Advantage：1）Degradation of pesticide bacterium solution can fully be adsorbed by net dirty carrier；2）Degradation bacteria liquid recycle stream moves speed can flexible modulation；3）Effectively bacterium solution is avoided to waste；4）Suitable for various shapes and the net dirty carrier of type.
Description
Technical field
The present invention is to be related to a kind of pesticide degradation bacteria and carrier loaded device, concretely relates to a kind of staged agricultural chemicals
Degradation bacteria and net dirty carrier loop flowing load device, belong to agricultural non-point source pollution control technology field.
Background technology
Agricultural chemicals plays an important roll as a kind of important capital goods in the agricultural sector in crop yield and the prevention and control of plant diseases, pest control.
However, because Pesticide use lacks the control measures of systematization, standardization, China's agricultural chemicals is in the stage of being excessively used, actual
Agricultural chemicals utilization rate only has 30% or so, and a large amount of agricultural chemicals loss of loss turn into the important source of agricultural non-point source pollution in the environment,
Threaten the health of aquatic ecosystem；In numerous technologies of pollution by pesticides reparation, degradation bacteria reparation be considered as it is green,
Cost effective, most potential organic agricultural chemicals removal pathway；At present by taming, screening, being enriched with and separating from sludge, dirt
A variety of organic agricultural chemicals degradation bacterias have been obtained in the environmental samples such as water, soil, deposit；Such as CN104250626A discloses one plant
Chlorpyrifos degrading bacteriaErwinia xishuaiensis SCU-B244T, it can effective degrading organic phosphor pesticides chlopyrifos.
The acclimation and screening technology of pesticide degradation bacteria relative maturity, but how will screen the pesticide degradation bacteria of acquisition in reality
Application during border is repaired but needs to be broken through；Degradation bacteria couples attachment techniques with net dirty carrier and can load the free degradation bacteria of script
Or limit to the net dirty specific space surface scope of carrier, and then applied in pollution amelioration in the original location.Net dirty carrier is most
Crop material, porous carbon material, porous brick etc. are selected, is mostly at present simple quiet by the method that degradation bacteria is loaded on net dirty carrier
Infusion method is put, dirty carrier will be immersed in only in the bacterium solution rich in pesticide degradation bacteria, using the absorption property of net dirty carrier by agriculture
Medicine degradation bacteria is adhered to and net dirty carrier surface and internal voids surface；Such as CN103102015A discloses one kind and utilizes immobilization
The method that degradation bacteria administers organophosphorus pesticide wastewater, it is characterized in that the organophosphorus pesticide degradation bacterium fungus Aspergillus niger obtained will be screened
Loaded to using simply method is standing and soak on activated carbon, the activated carbon containing saturation aspergillus niger then will be made and put into organophosphor agriculture
Pollution amelioration is carried out in chemicals contamination water body；But this method has the following disadvantages：（1）During being standing and soak for, degradation bacteria can be by
Occur to settle in self gravitation factor and be gathered in container bottom, cause carrier adsorption uneven, often bottom degradation bacteria it is more and
Top degradation bacteria is few, influences the degradation bacteria-net repairing effect of dirty carrier complex in actual applications；（2）The drop largely deposited
Bacterium is solved not in suspended state, thus can not effectively be adsorbed by net dirty carrier, reduces load effect of the net dirty carrier to degradation bacteria
Rate and load biomass；（3）Because infuser device is typically not easy to recovery renewal, great Liang Wei with the single container such as tank, bacterium solution
Adsorbed degradation bacteria loss and waste；（4）It is difficult to be applied to various shapes and the net dirty carrier of type, if pelleted substrate is negative
Not easily collecting after load, and exist efficiency it is low, can not same time, high-volume carry out the technological deficiencies such as degradation bacteria load.
The content of the invention
Proposed by the present invention is that a kind of staged pesticide degradation bacteria flows load device, its purpose purport with net dirty carrier loop
Solve existing pesticide degradation bacteria with it is net it is dirty it is carrier loaded present in problem, improve net dirty carrier loaded efficiency and applicability.
The technical solution of the present invention：Staged pesticide degradation bacteria and net dirty carrier loop flowing load device, it is tied
Structure includes adsorption box 1, discharge opeing case 6, catch box 13, flow speed control valve 5, staged fixed mount 16；Wherein, if staged fixed mount 16 has
Dry step, the bottom of staged fixed mount 16 have catch box 13, and the top of staged fixed mount 16 has discharge opeing case 6, and staged is solid
Determine have adsorption box 1 on each step among frame 16, the bottom of discharge opeing case 6 and the side of adjacent adsorption box 1 pass through connecting tube 4
It is connected, the bottom of each adsorption box 1 is connected with the side of the adsorption box 1 on next layer of step by connecting tube 4, bottom one
The bottom of adsorption box 1 is connected with the side of catch box 13 by connecting tube 4.
Advantages of the present invention：
1）Multiple adsorption boxes can be classified by the staged fixed mount designed by the present invention to be arranged, and this structure can make bacterium solution in adsorption box
Flowed by Action of Gravity Field in adsorption boxes at different levels, effectively avoid the pesticide degradation bacteria caused by static bacterium solution infusion method from settling,
The generation of carrier adsorption non-uniform phenomenon；
2）Stirring fan is provided with discharge opeing case, stirring fan is opened in adsorption process, bacterium solution disturbance can make discharge opeing case Pesticides degrade
Bacterium suspends, and promotes pesticide degradation bacteria to be uniformly distributed in bacterium solution, so as to ensure to flow out to the bacterium solution Pesticides degradation bacteria of adsorption box
It is uniformly distributed, the appearance of bad adsorption effect caused by further avoiding pesticide degradation bacteria sedimentation；
3）Afterbody catch box is connected by the present invention with first order discharge opeing case by connecting tube, and centre is connected with drawing liquid pump, can
Bacterium solution is passed through into drawing liquid pumped back top discharge opeing case from bottom catch box.This structure can pass through pesticide degradation bacteria not to be adsorbed
The pump housing comes back to device initiating terminal with bacterium solution, so as to reenter adsorption system, is further adsorbed by carrier, forms circulation
Load model is flowed, effectively prevent the waste of pesticide degradation bacteria；
4）Be equipped with flow speed control valve between discharge opeing case of the present invention, catch box and every grade of adsorption box, can according to different strain, different net dirty carry
Body type adjustment bacterium solution circulates speed, net load of the dirty carrier to pesticide degradation bacteria is reached optimal adsorption effect；
5）The present invention is not limited to the adsorption process of single net dirty bearer type, and can be according to different carrier forms, from not
Same carrying method, more specific aim and eurytopicity；For example, when net dirty carrier is graininess, carrier can be loaded the present invention
Absorbent packet in, by absorbent packet be fixed on carrier rack absorbent packet hook on, absorbent packet be provided with small hole bacterium solution can be made to circulate；
If carrier is the large volume porous blocks such as porous brick dirty carrier only, can be directly placed into adsorption box, it is simple to operate, can
Same time, high efficiency, the loading process that pesticide degradation bacteria is in high volume completed on multi-stage stairs.
Brief description of the drawings
Accompanying drawing 1 is the overall structure diagram of the present invention.
Accompanying drawing 2 is the partial schematic diagram of staged adsorptive racks of the present invention.
Accompanying drawing 3 is the dimensional structure diagram of adsorption box of the present invention.
Accompanying drawing 4 is the structural representation of carrier rack of the present invention.
1 is adsorption box in accompanying drawing, and 2 be absorption case lid, and 3 be connector, and 4 be connecting tube, and 5 be flow speed control valve, and 6 be discharge opeing case, 7
It is discharge opeing case lid, 8 be stirring fan, and 9 be carrier rack, and 10 be absorbent packet hook, and 11 be absorbent packet, and 12 be carrier rack slot, and 13 be collection
Liquid case, 14 be liquid collecting case lid, and 15 be drawing liquid pump, and 16 be staged fixed mount.
Embodiment
Accompanying drawing 1 is compareed, a kind of staged pesticide degradation bacteria includes inhaling with net dirty carrier loop flowing load device, its structure
Attached case 1, discharge opeing case 6, catch box 13, drawing liquid pump 15, flow speed control valve 5, staged fixed mount 16；Wherein, staged fixed mount 16 has
Some steps, the bottom of staged fixed mount 16 have catch box 13, and the top of staged fixed mount 16 has discharge opeing case 6, staged
There is adsorption box 1 on each step among fixed mount 16, the bottom of discharge opeing case 6 passes through with the side of adjacent adsorption box 1 to be connected
Pipe 4 is connected, and the bottom of each adsorption box 1 is connected with the side of the adsorption box 1 on next layer of step by connecting tube 4, bottom
The bottom of one adsorption box 1 is connected with the side of catch box 13 by connecting tube 4.
Bottom, the bottom of each adsorption box 1, the side of each adsorption box 1, the side of catch box 13 of the discharge opeing case 6
Respectively there is a connector；Between the connector of the side of the connector of the bottom of discharge opeing case 6 and adjacent adsorption box 1, each absorption
On the connector of the bottom of case 1 and next layer of step between the connector of the side of adsorption box 1, the connection of the bottom of bottom adsorption box 1
Mouthful be connected between the connector of the side of catch box 13 by identical connecting tube 4, have flow speed control valve 5 in every connecting tube 4,
Speed is circulated by bacterium solution in the control device of flow speed control valve 5, the net dirty carrier and the regulation of degradation bacteria species in device are each
The control of flow speed control valve 5 between level circulates speed, so that net load of the dirty carrier to pesticide degradation bacteria reaches optimum efficiency；Institute
The effect for stating discharge opeing case 6 is to provide the bacterium solution rich in effective degradation bacteria for lower section adsorption boxes 1 at different levels, while is collected in catch box 13
Remaining bacterium solution；When the stirring fan 8 is opened, ensure bacterium solution homogeneity, prevent pesticide degradation bacteria from settling, make adsorption boxes 1 at different levels
Pesticides degradation bacteria is uniformly distributed.
The top of discharge opeing case 6 is provided with the discharge opeing case lid 7 to match with the size of discharge opeing case 6.
Stirring fan 8 is provided with the discharge opeing case 6, stirring fan 8 is opened during carrier adsorption, ensures degradation of pesticide bacterium solution
Homogeneity, avoid settling.
The inner bottom part of adsorption box 1 is provided with carrier rack slot 12, and carrier rack 9 is provided with carrier rack slot 1；The carrier rack 9
It is provided with absorbent packet hook 10 to be used to hang fixed absorbent packet 11, when net dirty carrier is graininess, net dirty carrier is put into suction
Reach the purpose of fixed net dirt carrier in pocket, this structure is easy to the recycling of net dirty carrier, prevents unnecessary graininess
Net dirty carrier is wasted, and facilitating graininess, taking for dirty carrier is deposited and collected only.
The absorbent packet 11 is provided with small hole, degradation bacteria can free flow it is abundant through absorbent packet 11 and dirty carrier only
Contact, the aperture of small hole are less than the diameter of net dirty carrier.
The top of catch box 13 is provided with liquid collecting case lid 14 corresponding with the size of catch box 13；
The bottom of the catch box 13, the side of discharge opeing case 6 are provided with and the bottom connector identical connector of discharge opeing case 6, liquid collecting
The connector 4 of the bottom of case 13 is connected with one end of drawing liquid pump 15 by connecting tube 4, the other end and the discharge opeing case 6 of drawing liquid pump 15
Side connector is connected by same connector 4, is reached degradation bacteria liquid recycle stream and is moved loop.
The catch box 13 lays the bottommost of staged fixed mount 16；The catch box side is provided with connector 3, can lead to
Connecting tube 4 is crossed with higher level's adsorption box 1 to be connected；The bottom of catch box 13 is provided with connector 3, passes through connecting tube 4 and drawing liquid pump 15
It is connected；The opposite side of drawing liquid pump 15 is connected with the side connector 3 of discharge opeing case 6；After plant running, received when in catch box 13
When collection to casing accumulates 1/2 bacterium solution, drawing liquid pump 15 is opened, the bacterium solution in catch box 13 is withdrawn into discharge opeing case 6, forms bacterium solution
Circulation loop, promotes system Pesticides degradation bacteria fully to be adsorbed by carrier, effectively avoids the waste of degradation bacteria.
The staged fixed mount 16 is used to fix discharge opeing case 6, adsorption box 1, catch box 13；In use, will arrange
Liquid case 6 is positioned over the top of staged fixed mount 16, provides bacterium solution to be adsorbed for lower section adsorption boxes 1 at different levels, by adsorption box 1
It is placed according to demand selection suitable quantity classification on the step of the different height of staged fixed mount 16, catch box 13 is placed in ladder
The lowermost end of formula fixed mount 16, when device operates, bacterium solution can flow through every one-level adsorption box 1 by Action of Gravity Field, finally flow into
Catch box 13 flows back to discharge opeing case 6 by drawing liquid pump 15 again, completes bacterium solution circulation.
Accompanying drawing 1,3 is compareed, the adsorption box 1 is located at the lower section of top discharge opeing case 6, and multistage is placed in staged fixed mount 16
On；The top of adsorption box 1 is provided with dismountable absorption case lid 2；The side of adsorption box 1 is provided with connector 3, can pass through connection
Pipe 4 is connected with discharge opeing case 6 or higher level's adsorption box 1；The bottom of adsorption box 1 is provided with connector 3, can pass through connecting tube 4 and subordinate
Adsorption box 1 or catch box 13 are connected；This structure can promote bacterium solution in a device by Action of Gravity Field circulation staged fixed mount 16
On every one-level adsorption box 1, circulated because bacterium solution is between adsorption boxes 1 at different levels, degradation of pesticide bacterium solution is in whole system
In in homogenizing, ensure pesticide degradation bacteria uniform load on net dirty carrier.
All connecting tubes 4 are identical connecting tube in the present invention.
The present invention can make up the blank of existing net dirty material load device, can effectively solve agricultural chemicals solution bacterium in carrier adsorption mistake
In journey the problems such as skewness, free settling.
The present invention is described in further detail with reference to embodiment：
Embodiment 1
The method that activated carbon dirty material only is obtained using staged pesticide degradation bacteria and net dirty carrier loop flowing load device, bag
Include following steps：
1）The net dirty carrier of choosing is granular active carbon, and granular active carbon is fitted into absorbent packet 11, absorbent packet 11 is hung solid
Due on the absorbent packet hook 10 of carrier rack 9, carrier rack 9 and the interior carrier rack slot 12 of adsorption box 1 are connect relatively, carried so as to fixed
Body frame 9；
2）Pesticide degradation bacteria selects organophosphorus pesticide chlorpyrifos degrading bacteriaSphingomonas sp. Dsp-2, by organophosphorus pesticide poison
Dead tick degradation bacteriaSphingomonas sp.Dsp-2 is inoculated in 10 L 1/5LB culture mediums（Include in 1/5 LB culture mediums
The g/L of yeast extract 1, the g/L of tryptone 2, sodium chloride 2 g/L, pH 7.0）Obtain bacterium solution of degrading；
3）The degraded bacterium solution that will be enriched in pesticide degradation bacteria is poured into discharge opeing case 6, opens stirring fan 8, while by the speed governing of at different levels
Valve 5 is adjusted to maximum gear, bacterium solution is flowed through adsorption boxes at different levels, when bacterium solution liquid level of being degraded in every one-level adsorption box flooded carrier
During frame 9, stop toppling over bacterium solution into discharge opeing case 6, now rotate flow speed control valve 5, it is 0.5 L/ to make every grade of degradation bacteria liquid flowing velocity
min；When collection has the i.e. 4 L bacterium solution of 1/2 volume in catch box 13, drawing liquid pump 15 is opened, sends degraded bacterium solution back to discharge opeing case 6
In, go into the dynamic purpose of degradation bacteria liquid recycle stream；
5）After bacterium solution of degrading circulates 2~3 times, degradation bacteria absorption is completed, and now carrier rack 9 can be taken out from adsorption box 1, taken
Lower absorbent packet 11, granulated activated high-area carbon is taken out from absorbent packet 11, so as to obtain loading organophosphorus pesticide chlopyrifos drop
Solve bacteriumSphingomonas sp. Dsp-2 activated carbon dirty material only.
Adsorption box 1 described in the present embodiment, discharge opeing case 6, the material of catch box 13 are lucite, and the size of adsorption box 1 is
15 × 15 × 20 cm, the discharge opeing case 6 and cm of 13 size of catch box 20 × 20 × 20；The model FKC-G4-02B of flow speed control valve 5 or
FNC-G02-4 flow control valve；The staged fixed mount 16 selects stainless steel, if six grades of ladders, first order frame is high
For 1.2 m, thereafter per one-level frame height 20 cm lower than upper level；The material of carrier rack 9 is rigid plastics, the frame of carrier rack 9 a height of 15
cm。
Embodiment 2
It is a kind of to carry out the net dirty load of porous coagulation fragment of brick using staged pesticide degradation bacteria and dirty carrier loop flowing load device only
The specific method of body absorption organophosphorus pesticide degradation bacterium is as follows：
1）It is organophosphorus pesticide chlorpyrifos degrading bacteria to select pesticide degradation bacteriaSphingomonas sp. Dsp-2, prepare degradation bacteria
Liquid, the compound method for bacterium solution of degrading are same as Example 1；
2）Porous coagulation fragment of brick is chosen as net dirty carrier, porous coagulation fragment of brick cube that dirty carrier is 10 × 10 × 10 cm only
Body, this net dirty carrier is directly placed into the bottom of adsorption box 1, the degraded bacterium solution that will be enriched in pesticide degradation bacteria is poured into discharge opeing case 6, is opened
Open stirring fan 8, while the flow speed control valve 5 of at different levels be adjusted to maximum gear, when degraded bacterium solution flood it is more in every one-level adsorption box
Only dirty carrier is that degraded bacterium solution is toppled in stopping to hole coagulation fragment of brick, and flow speed control valve 5 now is adjusted into 0.3 L/min gears, makes degradation bacteria
Liquid flows in a device, when collection has the i.e. 4 L bacterium solution of 1/2 volume in catch box 13, drawing liquid pump 15 is opened, by bacterium solution of degrading
Send back in discharge opeing case 6, the purpose that row moves into degradation bacteria liquid recycle stream；
3）After bacterium solution circulates 3~4 times, absorption is completed, and block porous brick dirty carrier only can be now taken out from adsorption box 1, so as to
Obtain being loaded with the block porous brick of organophosphorus pesticide chlorpyrifos degrading bacteria dirty material only.
Adsorption box 1 described in the present embodiment, discharge opeing case 6, casing material, the size of catch box 13, the material of staged fixed mount 16
Matter, size, the type of flow speed control valve 5 are in the same manner as in Example 1.
Claims (9)
1. staged pesticide degradation bacteria and net dirty carrier loop flowing load device, it is characterized in that including adsorption box, discharge opeing case, collection
Liquid case, flow speed control valve, staged fixed mount；Wherein, staged fixed mount has some steps, and the staged fixed mount bottom has liquid collecting
Case, staged fixed mount top have a discharge opeing case, have adsorption box, the bottom of discharge opeing case on each step among staged fixed mount
Portion is connected with the side of adjacent adsorption box by connecting tube, the bottom of each adsorption box and the adsorption box on next layer of step
Side is connected by connecting tube, and the bottom bottom of an adsorption box is connected with the side of catch box by connecting tube.
2. staged pesticide degradation bacteria according to claim 1 and net dirty carrier loop flowing load device, it is characterized in that
Respectively there is a connection bottom, the bottom of each adsorption box, the side of each adsorption box, the side of catch box of the discharge opeing case
Mouthful；Between the connector in discharge opeing bottom portion and the connector of the side of adjacent adsorption box, the connector of each adsorption box bottom
Between the connector of adsorption box side on next layer of step, the company of the connector of bottom adsorption box bottom and catch box side
It is connected between interface by identical connecting tube, has flow speed control valve in every connecting tube.
3. staged pesticide degradation bacteria according to claim 2 and net dirty carrier loop flowing load device, it is characterized in that
Flow speed control valve control degradation bacteria liquid recycle stream moves speed, according to net dirty carrier and degradation bacteria species adjust it is at different levels between flow speed control valve
Control circulates speed, so that net load of the dirty carrier to pesticide degradation bacteria reaches optimum efficiency.
4. staged pesticide degradation bacteria according to claim 1 and net dirty carrier loop flowing load device, it is characterized in that
The effect of the discharge opeing case is to provide the bacterium solution rich in effective degradation bacteria for lower section adsorption boxes at different levels, while collects in catch box and remain
Remaining degraded bacterium solution.
5. staged pesticide degradation bacteria according to claim 1 and net dirty carrier loop flowing load device, it is characterized in that
Stirring fan is provided with the discharge opeing case, stirring fan is opened during carrier adsorption, ensures degradation of pesticide bacterium solution homogeneity, avoids
Settle.
6. staged pesticide degradation bacteria according to claim 1 and net dirty carrier loop flowing load device, it is characterized in that
The adsorption box inner bottom part is provided with carrier rack slot, and carrier rack is provided with carrier rack slot；The carrier rack is hung provided with absorbent packet
Hook is used to hang fixed absorbent packet, when net dirty carrier is graininess, net dirty carrier is put into absorbent packet and reaches fixed net dirt
The purpose of carrier, it is easy to the recycling of net dirty carrier, prevents that dirty carrier wastes unnecessary graininess only, facilitates graininess net
Taking for dirty carrier is deposited and collected.
7. staged pesticide degradation bacteria according to claim 1 and net dirty carrier loop flowing load device, it is characterized in that
The bottom of the catch box, the side of discharge opeing case be provided with discharge opeing bottom portion connector identical connector, catch box bottom
Connector is connected with one end of drawing liquid pump by connecting tube, and the other end of drawing liquid pump passes through same with the side connector of discharge opeing case
Connector be connected, reach degradation bacteria liquid recycle stream move loop.
8. staged pesticide degradation bacteria according to claim 6 and net dirty carrier loop flowing load device, it is characterized in that
The absorbent packet is provided with small hole, degradation bacteria can free flow fully contacted with dirty carrier only through absorbent packet, micro hole
The aperture in hole is less than the diameter of net dirty carrier.
9. staged pesticide degradation bacteria according to claim 7 and net dirty carrier loop flowing load device, it is characterized in that
When having been collected in the catch box to 1/2 degraded bacterium solution of casing product, drawing liquid pump is opened, the bacterium solution in catch box is withdrawn into discharge opeing
In case, bacterium solution is formed circulation loop, promote system Pesticides degradation bacteria fully to be adsorbed by carrier, effectively avoid the wave of degradation bacteria
Take.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710571499.5A CN107365689A (en) | 2017-07-13 | 2017-07-13 | A kind of staged pesticide degradation bacteria and net dirty carrier loop flowing load device |
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| CN201710571499.5A CN107365689A (en) | 2017-07-13 | 2017-07-13 | A kind of staged pesticide degradation bacteria and net dirty carrier loop flowing load device |
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| CN101550393A (en) * | 2009-05-06 | 2009-10-07 | 兆凯生物工程研发中心（深圳）有限公司 | Reactor for microalgae outdoor breeding |
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| CN1033644A (en) * | 1987-12-22 | 1989-07-05 | 中国科学院成都生物研究所 | A kind of preparation of immobilization caproic acid bacteria and be applied to the novel method of fragrant type brewing brew |
| CN101550393A (en) * | 2009-05-06 | 2009-10-07 | 兆凯生物工程研发中心（深圳）有限公司 | Reactor for microalgae outdoor breeding |
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