CN104293664A - Space application type flow-path-controllable locked cell culture chamber - Google Patents
Space application type flow-path-controllable locked cell culture chamber Download PDFInfo
- Publication number
- CN104293664A CN104293664A CN201410389390.6A CN201410389390A CN104293664A CN 104293664 A CN104293664 A CN 104293664A CN 201410389390 A CN201410389390 A CN 201410389390A CN 104293664 A CN104293664 A CN 104293664A
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- CN
- China
- Prior art keywords
- cell culture
- culture chamber
- opening
- locking block
- push rod
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
Abstract
The invention discloses an enclosed cell culture chamber inside provided with a liquid flowing flow path control structure and an opening locking structure. The cell culture chamber possesses the control capability on a flowing flow path when a reagent is injected, and also is capable of automatically exhausting air for again injecting a reagent after evacuation. Also, the opening locking structure is capable of closing an opening after a reagent is injected or extracted and putting an end to reagent leakage in the cell culture chamber, so that the cell culture chamber is usable under the conditions of a space microgravity environment in an automatic cell culture apparatus. The cell culture chamber is characterized in that the chamber comprises cell culture membranes, a cell culture chamber main body, an opening locking block, a locking-block push rod and an elastic rubber plug; the cell culture membranes are installed at two sides of the cell culture chamber main body, and the opening locking block, the locking-block push rod and the elastic rubber plug are assembled at installation positions at the frame of the cell culture chamber; and the cell culture chamber main body comprises the frame, a flow-path blocking dam, a locking-block push rod installation hole, an opening locking block chute and an opening.
Description
Technical field the present invention relates to a kind of cell culture chamber being applied to cell biology and life science, particularly one possesses stream control when nutrient solution flows, opening is with safety locking structure, can be applicable to the automatic culture apparatus of cell, the hermetic type cell culture chamber of space microgravity envrionment conditions can be adapted to.
Background technology is in RESEARCH ON CELL-BIOLOGY field and life science field, and cell cultures is the most basic link.At present, in laboratory, cell cultures commonly uses Tissue Culture Flask or Tissue Culture Plate, and airtight cell culture chamber is only for objects such as cell sample transports.For OptiCell1100 type cell culture chamber conventional on market, this airtight cell culture chamber has a larger cell culture area without internal structure, and opening only uses plug filling.The cell culture chamber of this structure is not enough as follows:
1, the empty maxicell culturing room of this structure without internal structure annotate cell culture fluid or in fact reagent time, flow path of the liquid is uncontrolled, affect reagent replacing efficiency; Often air can not be drained when horizontality annotates reagent again after particularly emptying, in the indoor formation larger bubble of cell cultures, affect cell normal growth.Therefore, this cell culture chamber is not suitable for the automatic culture apparatus of cell, also meet have emptying after annotate again reagent requirement Space Experiments use.
2, the opening of this cell culture chamber is not owing to having lock structure, and fill-type plug declines in sealing property after repeatedly syringe needle penetrates filling reagent operation, causes nutrient solution or reagent seepage in cell culture chamber.This is also that Space Experiments is unallowed.
Summary of the invention the object of the invention is to set up the airtight cell culture chamber of a kind of inside with liquid flow stream pipelines control structure and opening lock structure, flowing stream controllability when both having possessed filling reagent, also can annotate again after emptying reagent time automatically drain air; Meanwhile, the lock structure of opening can complete reagent filling or after extracting, split interruption-forming and close, stop reagent seepage in cell culture chamber, thus meets and use under space microgravity envrionment conditions He in automatic cell culture apparatus.
The present invention includes cell cultures film, cell culture chamber main body, opening locking block, locking block push rod and elastic plug five part, cell cultures film is installed on cell culture chamber main body both sides, imports and exports locking block, locking block push rod and elastic plug and is assembled in cell culture chamber frame installation site.
Described cell culture chamber main body comprises frame, stream stops dam, locking block push rod open holes, opening locking block chute and opening.2 openings lay respectively at along two ends under frame, and each open bottom all has opening locking block chute; Porose through with scuncheon bottom opening locking block chute; Frame two side lower part all has 1 locking block push rod open holes; Stream stops that dam is distributed in the middle of frame.
2 respectively cell cultures film be bonded in cell culture chamber main body both sides, 2 opening locking blocks are installed on opening locking block chute outer end respectively, make opening locking block centre hole and opening locking block chute base apertures through, 2 elastic plugs are that compressed state is installed in opening respectively, and 2 locking block push rods are assembled in frame respectively by locking block push rod open holes.After such assembling at cell culture chamber main body intermediate formation one close and stop that dam can control the region of reagent flows with stream, can cell cultures be carried out in this region.
After the present invention adopts technique scheme, its distinguishing feature is:
1, the cell cultures zone closed has the stream of reagent flows stream controlling functions to stop dam, can in reagent filling process, make reagent by set flow path, efficiency changed by effective raising reagent, also effectively can get rid of the bubble of cell culture area simultaneously, the present invention be met and uses under microgravity environment He in automatic cell culture apparatus.
2, in opening except after being filled with elastic plug, also there is the opening locking block of sealing function, can locking opening more reliably, stop reagent seepage, meeting spatial requirement of experiment.
Description of drawings 1 structural representation of the present invention
Fig. 2 cell culture chamber agent structure of the present invention schematic diagram
Embodiment cell culture chamber main body 2 adopts medical transparent polycarbonate material injection moulding, the long 90mm of physical dimension, wide 55mm, and thickness is 7mm; Frame 21 is recessed into thickness 2mm in centre, and arrange 3 streams in the middle of frame 21 and stop dam 22, thickness is 2mm also, and wide is 1mm; Cell cultures film 1 adopts medical transparent polycarbonate material calendering formation, and thickness is 0.075mm, is welded on the both sides, frame 21 middle part of cell culture chamber main body 2 by HF plastic weldprocedure.
Opening locking block 4 utilizes polyfluortetraethylene plate machining, long 7mm, wide 3mm, thick 2.5mm, centre reagent flows bore dia 1mm.Locking block push rod 3 adopts Nylon-1010 injection moulding, and squared ends is of a size of 5mm × 7mm, locking block rod diameter 1.8mm.On frame 21, locking block push rod open holes 23 external parties hole dimension is 5.2mm × 7.2mm, outside push rod slip bore dia 2mm.Locking block push rod 3 promotes opening locking block 4 in opening locking block chute 24 from outside to inner slide by locking block push rod open holes 23.
Elastic plug 5 adopts silicon rubber mould molded, and main part physical dimension is 19.5 × 6 × 6 (units: mm), internal surface step height 5.5mm, material hardness HA45.Elastic plug 5 physical dimension is slightly larger than main body 2 upper shed 25 size, and elastic plug 5 is filled in opening 25 Central Mountain, and energy tensioner in the opening 25, can not come off.
After the present invention has assembled, can adopt Co60 radiation or fumigation stand-by.During use, utilize syringe needle to penetrate elastic plug 5, cell in syringe and nutrient solution are pushed cell culture chamber main body 2 intermediate cell and cultivate region, on another elastic plug 5, insert a syringe needle exhaust simultaneously.After cell culture chamber main body 2 intermediate cell cultivation region fills, pull out syringe and exhaust syringe needle, just the present invention can be put into cell culture incubator culturing cell.In experimentation, extract by syringe equally or change internal media of the present invention.After having tested, manual promotion locking block push rod 3, make opening locking block 4 in opening locking block chute 24 from outside to inner slide, make the centre hole of opening locking block 4 and opening locking block chute 24 base apertures misplace and close, stop nutrient solution or other reagent seepage.
Claims (1)
1. inside is with an airtight cell culture chamber for liquid flow stream pipelines control structure and opening lock structure, comprises cell cultures film, cell culture chamber main body, opening locking block, locking block push rod and elastic plug five part; Described cell cultures film is installed on cell culture chamber main body both sides, imports and exports locking block, locking block push rod and elastic plug and is assembled in cell culture chamber main body installation site; Described cell culture chamber main body comprises frame, stream stops dam, locking block push rod open holes, opening locking block chute and opening, 2 openings lay respectively under frame along two ends, each open bottom all has opening locking block chute, porose through with scuncheon bottom opening locking block chute, frame two side lower part all has 1 locking block push rod open holes; Stream stops that dam is distributed in the middle of frame.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201410389390.6A CN104293664B (en) | 2014-08-07 | 2014-08-07 | The applied stream in space controlled locked cell culture chamber |
Applications Claiming Priority (1)
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CN201410389390.6A CN104293664B (en) | 2014-08-07 | 2014-08-07 | The applied stream in space controlled locked cell culture chamber |
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CN104293664A true CN104293664A (en) | 2015-01-21 |
CN104293664B CN104293664B (en) | 2016-12-07 |
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CN201410389390.6A Active CN104293664B (en) | 2014-08-07 | 2014-08-07 | The applied stream in space controlled locked cell culture chamber |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104845888A (en) * | 2015-02-13 | 2015-08-19 | 中国航天员科研训练中心 | Totally-enclosed cell culture plate |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN2063190U (en) * | 1989-12-15 | 1990-10-03 | 胡福良 | Push-rod type reversal valve |
CN101541428A (en) * | 2006-09-07 | 2009-09-23 | 纳格-南科国际有限公司 | Culture dish with lid |
CN102260629A (en) * | 2010-05-28 | 2011-11-30 | 中国科学院过程工程研究所 | Plate-type photobioreactor |
CN203976812U (en) * | 2014-08-07 | 2014-12-03 | 中国航天员科研训练中心 | The controlled locked cell culture chamber of the applied stream in space |
-
2014
- 2014-08-07 CN CN201410389390.6A patent/CN104293664B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN2063190U (en) * | 1989-12-15 | 1990-10-03 | 胡福良 | Push-rod type reversal valve |
CN101541428A (en) * | 2006-09-07 | 2009-09-23 | 纳格-南科国际有限公司 | Culture dish with lid |
CN102260629A (en) * | 2010-05-28 | 2011-11-30 | 中国科学院过程工程研究所 | Plate-type photobioreactor |
CN203976812U (en) * | 2014-08-07 | 2014-12-03 | 中国航天员科研训练中心 | The controlled locked cell culture chamber of the applied stream in space |
Non-Patent Citations (1)
Title |
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王春艳等: "多坝式空间细胞培养室设计参数的微重力实验验证", 《航天医学与医学工程》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104845888A (en) * | 2015-02-13 | 2015-08-19 | 中国航天员科研训练中心 | Totally-enclosed cell culture plate |
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CN104293664B (en) | 2016-12-07 |
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