CN203976812U - The controlled locked cell culture chamber of the applied stream in space - Google Patents
The controlled locked cell culture chamber of the applied stream in space Download PDFInfo
- Publication number
- CN203976812U CN203976812U CN201420444381.8U CN201420444381U CN203976812U CN 203976812 U CN203976812 U CN 203976812U CN 201420444381 U CN201420444381 U CN 201420444381U CN 203976812 U CN203976812 U CN 203976812U
- Authority
- CN
- China
- Prior art keywords
- locking piece
- cell culture
- culture chamber
- opening
- push rod
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 54
- 239000007788 liquid Substances 0.000 claims abstract description 4
- 230000008676 import Effects 0.000 claims abstract description 3
- 238000009434 installation Methods 0.000 claims abstract description 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 22
- 238000007789 sealing Methods 0.000 abstract description 6
- 230000005486 microgravity Effects 0.000 abstract description 4
- 235000015097 nutrients Nutrition 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 238000001746 injection moulding Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 238000003490 calendering Methods 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005429 filling process Methods 0.000 description 1
- 238000003958 fumigation Methods 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 238000004023 plastic welding Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Inside is with an airtight cell culture chamber for liquid flow stream pipelines control structure and opening lock structure, and the stream controllability that flows while both having possessed filling reagent, drains air automatically also can annotate reagent after emptying time again; Meanwhile, the lock structure of opening can, after completing reagent filling or extracting, be split interruption-forming sealing, stops reagent seepage in cell culture chamber, uses thereby meet under space microgravity envrionment conditions He in automatic cell culture apparatus.It is characterized in that comprising cell cultures film, cell culture chamber main body, opening locking piece, locking piece push rod and elastic plug five parts, cell cultures film is installed on cell culture chamber main body both sides, imports and exports locking piece, locking piece push rod and elastic plug and is assembled in cell culture chamber frame installation site.Cell culture chamber main body comprises frame, stream stops dam, locking piece push rod open holes, opening locking piece chute and opening.
Description
Technical field the utility model relates to a kind of cell culture chamber that is applied to cytobiology field and life science, stream control when particularly one possesses nutrient solution and flows, opening is with safety locking structure, can be applicable to the automatic culture apparatus of cell, can adapt to the hermetic type cell culture chamber of space microgravity envrionment conditions.
Background technology is in RESEARCH ON CELL-BIOLOGY field and life science field, and cell cultures is the most basic link.At present, the conventional Tissue Culture Flask of cell cultures or Tissue Culture Plate in laboratory, airtight cell culture chamber is only for objects such as cell sample transports.Taking OptiCell1100 type cell culture chamber conventional on market as example, this airtight cell culture chamber has a larger cell cultures region without internal structure, and opening is only used plug filling.The cell culture chamber of this structure is not enough as follows:
1, the empty maxicell of this structure without internal structure culturing room is at filling cell culture fluid or in fact when reagent, and flow path of the liquid is uncontrolled, affect reagent replacing efficiency; After particularly emptying, in the time that horizontality is annotated reagent again, often air can not be drained, in the indoor formation larger bubble of cell cultures, affect cell normal growth.Therefore, this cell culture chamber is not suitable for the automatic culture apparatus of cell, does not meet yet and has the Space Experiments of the reagent requirement of annotating again after emptying to use.
2, the opening of this cell culture chamber is owing to there is no lock structure, and fill-type plug declines in sealing property after repeatedly syringe needle penetrates the operation of filling reagent, causes nutrient solution or reagent seepage in cell culture chamber.This is also that Space Experiments is unallowed.
Utility model content the purpose of this utility model is to set up the airtight cell culture chamber of a kind of inside with liquid flow stream pipelines control structure and opening lock structure, both while having possessed filling reagent, mobile stream controllability, drained air automatically also can annotate reagent after emptying time again; Meanwhile, the lock structure of opening can, after completing reagent filling or extracting, be split interruption-forming sealing, stops reagent seepage in cell culture chamber, uses thereby meet under space microgravity envrionment conditions He in automatic cell culture apparatus.
The utility model comprises cell cultures film, cell culture chamber main body, opening locking piece, locking piece push rod and elastic plug five parts, cell cultures film is installed on cell culture chamber main body both sides, imports and exports locking piece, locking piece push rod and elastic plug and is assembled in cell culture chamber frame installation site.
Described cell culture chamber main body comprises frame, stream stops dam, locking piece push rod open holes, opening locking piece chute and opening.2 openings lay respectively under frame along two ends, and each open bottom all has opening locking piece chute; Opening locking piece chute bottom is porose to be connected with scuncheon; Frame two side lower parts all have 1 locking piece push rod open holes; Stream stops that dam is distributed in the middle of frame.
2 respectively cell cultures film be bonded in cell culture chamber main body both sides, 2 opening locking pieces are installed on respectively opening locking piece chute outer end, opening locking piece centre hole and opening locking piece chute base apertures are connected, 2 elastic plugs are compressed state and are installed on respectively in opening, and 2 locking piece push rods are assembled in frame by locking piece push rod open holes respectively.Like this after assembling at cell culture chamber main body intermediate formation a sealing and stop that with stream dam can control the mobile region of reagent, in this region, can carry out cell cultures.
The utility model adopts after technique scheme, and its distinguishing feature is:
1, the cell cultures district of sealing stops dam with the stream of the mobile stream control function of reagent, can in reagent filling process, make reagent by set flow path, effectively improve reagent and change efficiency, also the bubble that can effectively get rid of cell cultures region, meets under microgravity environment He in automatic cell culture apparatus the utility model and uses simultaneously.
2, in opening except having filled after elastic plug, also there is the opening locking piece of sealing function, can be more locking opening reliably, stop reagent seepage, meeting spatial requirement of experiment.
Brief description of the drawings Fig. 1 the utility model structural representation
Fig. 2 cell culture chamber agent structure of the present utility model schematic diagram
Embodiment cell culture chamber main body 2 adopts medical transparent polycarbonate material injection moulding, the long 90mm of physical dimension, and wide 55mm, thickness is 7mm; Frame is recessed into thickness 2mm in 21 centres, in the middle of frame 21,3 streams is set and stops dam 22, and thickness is 2mm also, and wide is 1mm; Cell cultures film 1 adopts medical transparent polycarbonate material calendering formation, and thickness is 0.075mm, is welded on frame 21 both sides, middle part of cell culture chamber main body 2 by high frequency Plastic Welding technique.
Opening locking piece 4 utilizes polyfluortetraethylene plate machining moulding, long 7mm, wide 3mm, thick 2.5mm, centre reagent flow orifice diameter 1mm.Locking piece push rod 3 adopts Nylon-1010 injection moulding, and squared ends is of a size of 5mm × 7mm, locking piece rod diameter 1.8mm.On frame 21, locking piece push rod open holes 23 external parties hole dimensions are 5.2mm × 7.2mm, outside push rod slip bore dia 2mm.Locking piece push rod 3 by locking piece push rod open holes 23 promote opening locking piece 4 in opening locking piece chute 24 from outside to inner slide.
Elastic plug 5 adopts silicon rubber mould molded, and main part physical dimension is 19.5 × 6 × 6 (units: mm), the high 5.5mm of internal surface step, material hardness HA45.Elastic plug 5 physical dimension are slightly larger than main body 2 upper shed 25 sizes, and elastic plug 5 is filled in opening 25 Central Mountain, and energy tensioner, in opening 25, can not come off.
After the utility model has been assembled, can adopt Co60 radiation or fumigation stand-by.When use, utilize syringe needle to penetrate elastic plug 5, cell in syringe and nutrient solution are pushed to cell culture chamber main body 2 intermediate cells cultivation regions, on another elastic plug 5, insert a syringe needle exhaust simultaneously.After cell culture chamber main body 2 intermediate cells cultivation regions fill, pull out syringe and exhaust syringe needle, just the utility model can be put into cell culture incubator culturing cell.In experimentation, can extract or change the inner nutrient solution of the utility model by syringe equally.After having tested, manually promote locking piece push rod 3, make opening locking piece 4 in opening locking piece chute 24 from outside to inner slide, make the centre hole of opening locking piece 4 and the 24 base apertures dislocation of opening locking piece chute and seal, stop nutrient solution or other reagent seepage.
Claims (1)
1. inside, with an airtight cell culture chamber for liquid flow stream pipelines control structure and opening lock structure, comprises cell cultures film, cell culture chamber main body, opening locking piece, locking piece push rod and elastic plug five parts; Described cell cultures film is installed on cell culture chamber main body both sides, imports and exports locking piece, locking piece push rod and elastic plug and is assembled in cell culture chamber main body installation site; Described cell culture chamber main body comprises frame, stream stops dam, locking piece push rod open holes, opening locking piece chute and opening, 2 openings lay respectively under frame along two ends, each open bottom all has opening locking piece chute, opening locking piece chute bottom is porose to be connected with scuncheon, and frame two side lower parts all have 1 locking piece push rod open holes; Stream stops that dam is distributed in the middle of frame.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420444381.8U CN203976812U (en) | 2014-08-07 | 2014-08-07 | The controlled locked cell culture chamber of the applied stream in space |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420444381.8U CN203976812U (en) | 2014-08-07 | 2014-08-07 | The controlled locked cell culture chamber of the applied stream in space |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203976812U true CN203976812U (en) | 2014-12-03 |
Family
ID=51974274
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420444381.8U Expired - Lifetime CN203976812U (en) | 2014-08-07 | 2014-08-07 | The controlled locked cell culture chamber of the applied stream in space |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203976812U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293664A (en) * | 2014-08-07 | 2015-01-21 | 中国航天员科研训练中心 | Space application type flow-path-controllable locked cell culture chamber |
| CN105754856A (en) * | 2016-04-12 | 2016-07-13 | 中国航天员科研训练中心 | Drawer type three-dimensional quasi-vascularization tissue culture organ chip and establishment method thereof |
| CN105754855A (en) * | 2016-04-12 | 2016-07-13 | 中国航天员科研训练中心 | Fed-batch type two-layer cell co-culture chip |
-
2014
- 2014-08-07 CN CN201420444381.8U patent/CN203976812U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293664A (en) * | 2014-08-07 | 2015-01-21 | 中国航天员科研训练中心 | Space application type flow-path-controllable locked cell culture chamber |
| CN105754856A (en) * | 2016-04-12 | 2016-07-13 | 中国航天员科研训练中心 | Drawer type three-dimensional quasi-vascularization tissue culture organ chip and establishment method thereof |
| CN105754855A (en) * | 2016-04-12 | 2016-07-13 | 中国航天员科研训练中心 | Fed-batch type two-layer cell co-culture chip |
| CN105754856B (en) * | 2016-04-12 | 2018-06-22 | 中国航天员科研训练中心 | A kind of drawer type three-dimensional class blood vessel tissue culture organ chip and its construction method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20141203 |