CN115011474A

CN115011474A - Nutrient solution supply device for cell culture

Info

Publication number: CN115011474A
Application number: CN202210825184.XA
Authority: CN
Inventors: 柳安燕
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-07-14
Filing date: 2022-07-14
Publication date: 2022-09-06

Abstract

The invention discloses a nutrient solution supply device for cell culture, which comprises a supply box and a plurality of culture bottles, wherein the culture bottles are combined to form a cell culture box, a panel and a pressing plate are arranged in the supply box, the lower end of the cell culture box is inserted into the supply box, liquid injection pipes are vertically arranged at the centers of the inner bottom surfaces of the culture bottles, sealing rods are arranged in the liquid injection pipes, a plurality of liquid outlet holes are formed in the outer side surfaces of the liquid injection pipes, through holes are formed in the panel right opposite to the liquid injection holes, liquid supply pipes are arranged in the pressing plate right opposite to the liquid injection holes, a plurality of liquid supply ports are formed in the outer wall surface of each liquid supply pipe, and a sealing cavity is formed between the pressing plate and the supply box. The culture bottle feeding device is simple in structure, can supply nutrition to a plurality of culture bottles at one time, ensures that the nutrition liquid injected into each culture bottle is matched, greatly increases the efficiency, and ensures the safety of the culture bottles by avoiding the entry of external dust because the culture bottles are always in a sealed state in the feeding process.

Description

Nutrient solution supply device for cell culture

Technical Field

The invention relates to the technical field of cell culture, in particular to a nutrient solution supply device for cell culture.

Background

Cell culture refers to all in vitro cultures, and means that a tissue is taken out from a living body of an animal, the tissue is separated into single cells by a mechanical method or an enzymatic digestion method, and the single cells are incubated and cultured under a specific in vivo condition such as a simulated in vivo physiological environment to survive and grow. For the growth of cells, nutrient solution is required to be continuously supplied for cell culture.

The existing method for supplying nutrient solution for cell culture generally injects nutrient solution into culture bottles one by a liquid-transferring gun, and in the operation process, because the nutrient solution needs to be injected one by one, the operation process is long, so that a plurality of culture bottles are always in an open state, dirt such as dust is easy to enter, and the culture quality of cells is influenced.

Disclosure of Invention

The invention aims to solve the technical problem of providing a nutrient solution supply device for cell culture, which can complete liquid injection of a plurality of culture bottles at one time without opening the culture bottles in a liquid supply state and greatly improve the efficiency.

The invention is realized by the following technical scheme: a nutrient solution supply device for cell culture comprises a supply box and a plurality of culture bottles, wherein the culture bottles form a cell culture box in a combined manner, a panel and a pressing plate are arranged in the supply box, the panel is arranged above the pressing plate, the lower end of the cell culture box is inserted into the supply box and is in contact with the panel, the width and the length of the cell culture box are matched with the length and the width of the interior of the supply box, liquid injection pipes are vertically arranged at the centers of the bottom surfaces of the interior of the culture bottles, the upper ends of the liquid injection pipes are hermetically arranged, the interior of each liquid injection pipe is provided with a sealing rod, a plurality of liquid outlet holes are arranged on the outer side surfaces of the liquid injection pipes and are hermetically arranged by the sealing rods, liquid injection holes communicated with the liquid injection pipes are arranged on the bottom surfaces of the culture bottles, through holes are arranged on the panel right opposite to the liquid injection holes, liquid supply pipes are arranged on the pressing plate right opposite to the liquid injection holes, the upper ends of the liquid supply pipes are hermetically arranged, a plurality of liquid supply ports are arranged on the outer wall surfaces of the liquid supply pipes, liquid inlet holes are formed in the bottom surface of the pressing plate and opposite to the liquid supply pipe, and sealed cavities are formed between the pressing plate and the supply box.

As preferred technical scheme, all install the rubber layer on the outer wall of sealing rod, the rubber layer all contacts with the internal wall face of notes liquid pipe, and first spring is all installed to the sealing rod upper end, and the first spring other end all is installed on the inside top surface of notes liquid pipe.

As preferred technical scheme, all install the rubber frame on the side of clamp plate, the lateral surface of rubber frame touches with the internal face of feed box, the polylith slider is all installed to the both sides of clamp plate and panel, slider on the clamp plate all passes the rubber frame setting, the internal face of feed box all is equipped with the spout just to slider department, the equal two weak points in length of spout set up, the equal slidable mounting of slider on the panel is in longer spout, the equal slidable mounting of slider on the clamp plate is in shorter spout, all install the second spring on the bottom surface of slider, the other end of second spring is all installed on the bottom surface of spout, the cross-section of slider and spout all is the trapezium structure setting.

As the preferred technical scheme, a liquid feeding pipe communicated with the sealing cavity is installed on the outer side surface of the supply box, and the other end of the liquid feeding pipe is in threaded connection with a sealing cover.

As the preferred technical scheme, the upper ends of the culture bottles are all provided with a ventilating cover.

As a preferred technical scheme, scales are arranged on the culture bottle on one side.

Preferably, the supply box is made of a transparent medical plastic material.

The invention has the beneficial effects that: the culture box is simple in structure, the culture box can be inserted into the matched supply box, the liquid supply pipe can be inserted into the liquid injection pipe after the culture box is pressed down, the sealing rod is pushed away from the inner side of the liquid outlet hole, the liquid supply port is opposite to the liquid outlet hole, and after the culture box is pressed down continuously, nutrient solution in the sealing cavity can be discharged along the liquid supply pipe, the liquid injection pipe, the liquid supply port and the liquid outlet hole, so that the nutrient supply can be performed on a plurality of culture bottles at one time, the matching of the nutrient solution injected into each culture bottle is ensured, the efficiency is greatly improved, the culture bottles are always in a sealing state in the above supply process, the entering of external dust is avoided, and the safety of the culture bottles is ensured.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is an enlarged view of a portion of FIG. 1 at A;

fig. 3 is a vertical sectional view of the supply tank of the present invention.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.

All of the features disclosed in this specification, or all of the steps in any method or process so disclosed, may be combined in any combination, except combinations of features and/or steps that are mutually exclusive.

Any feature disclosed in this specification (including any accompanying claims, abstract and drawings), may be replaced by alternative features serving equivalent or similar purposes, unless expressly stated otherwise. That is, unless expressly stated otherwise, each feature is only an example of a generic series of equivalent or similar features.

As shown in FIG. 1, FIG. 2 and FIG. 3, the nutrient solution supply device for cell culture of the present invention comprises a supply box 2 and a plurality of culture bottles 1, wherein the culture bottles 1 are combined to form a cell culture box, a panel 3 and a pressing plate 4 are arranged in the supply box 2, the panel 3 is arranged above the pressing plate 4, the lower end of the cell culture box is inserted into the supply box 2 and contacts with the panel 3, the width and length of the cell culture box are matched with the length and width of the supply box 2, a liquid injection pipe 6 is vertically arranged at the center of the bottom surface in the culture bottle 1, the upper end of the liquid injection pipe 6 is hermetically arranged, a sealing rod 7 is arranged in the liquid injection pipe 6, a plurality of liquid outlet holes 18 are arranged on the outer side surface of the liquid injection pipe 6, the liquid outlet holes 18 are hermetically arranged by the sealing rod 7, liquid injection holes communicated with the liquid injection pipe are arranged on the bottom surface of the culture bottle 1, through holes 11 are arranged on the panel 3 opposite to the liquid injection holes, the pressure plate 4 is just all installed liquid supply pipe 12 to liquid injection hole department, and liquid supply pipe 12 upper end all seals up the setting, all is equipped with a plurality of liquid feed ports 13 on the outer wall of liquid supply pipe 12, just all is equipped with the liquid inlet hole to liquid supply pipe department on the bottom surface of pressure plate 4, all forms sealed chamber 5 between pressure plate 4 and the feed box 2.

In this embodiment, the outer wall surfaces of the sealing rods 7 are all provided with rubber layers 19, the rubber layers 19 are all in contact with the inner wall surface of the liquid injection pipe 6, the upper ends of the sealing rods 7 are all provided with first springs 8, and the other ends of the first springs 8 are all arranged on the top surface inside the liquid injection pipe 6.

In this embodiment, all install the rubber frame on the side of clamp plate 4, the lateral surface of rubber frame touches with the internal face of feed box 2, polylith slider 14 is all installed to clamp plate 4 and panel 3's both sides, the slider on the clamp plate 4 all passes the rubber frame setting, the internal face of feed box 2 is just all being equipped with spout 21 to slider department, spout 21 is two weak or two weak settings of equal length, the equal slidable mounting of slider on the panel 3 in longer spout, the equal slidable mounting of slider on the clamp plate 4 in shorter spout, all install second spring 15 on the bottom surface of slider 14, the other end of second spring 15 is all installed on the bottom surface of spout 21, the cross-section of slider 14 and spout 21 all is the trapezium structure setting.

In this embodiment, install the liquid feeding pipe 16 with sealed chamber intercommunication on the lateral surface of supply box 2, the other end threaded connection of liquid feeding pipe 16 has sealed lid 17, and with the supply box upset back, the liquid feeding pipe can set up for the nutrient solution can fill sealed chamber along the liquid feeding pipe, otherwise, after the liquid feeding pipe down, inside nutrient solution can pour out.

In the embodiment, the upper end of the culture bottle 1 is provided with the ventilating cover 9, and when liquid is injected, air in the culture bottle can be discharged along the ventilating cover, so that the influence on liquid injection is avoided.

In this embodiment, the culture bottle 1 on one side is provided with scales through which the amount of the nutrient solution to be injected can be controlled.

In this embodiment, the supply tank 2 is made of a transparent medical plastic material so that the volume of the internal nutrient solution can be seen through the supply tank.

The incubator can be inserted into the matched supply box to combine the incubator with the supply box, when nutrient solution needs to be added, the incubator can be directly pressed downwards to be adhered to the inner wall of the supply box and vertically downwards, after being pressed down, the liquid supply pipe can be inserted into the liquid injection pipe and push the sealing rod away from the inner side of the liquid outlet hole, so that the liquid supply port is opposite to the liquid outlet hole, after being pressed down continuously, the panel can be contacted with the pressing plate, so that the pressing plate can be driven to move downwards synchronously, the nutrient solution in the sealed cavity can be extruded out along the liquid supply pipe, the liquid injection pipe, the liquid supply port and the liquid outlet hole through the pressure plate, the nutrient supply can be carried out on a plurality of culture bottles at one time, the matching of the nutrient solution injected by each culture bottle is ensured, the efficiency is greatly increased, in addition, the culture bottles are always in a sealed state in the supply process, so that external dust is prevented from entering, and the safety of the culture bottles is ensured;

above-mentioned back of accomplishing, can release the incubator, at this moment, resilience ability panel and the clamp plate through the second spring are lifted, also make the incubator get back to the normal position again, and the feed pipe that inserts the incubator before can extract from cultivateing joining in marriage, can promote sealing rod downwards through the bounce-back of first spring, make the inside of moving to going out the liquid hole of sealing rod again to going out liquid hole is sealed again.

The above description is only an embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that are not thought of through the inventive work should be included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope defined by the claims.

Claims

1. A nutrient solution supply device for cell culture, characterized in that: comprises a supply box (2) and a plurality of culture bottles (1), the culture bottles (1) are combined to form a cell culture box, a panel (3) and a pressing plate (4) are arranged inside the supply box (2), the panel (3) is arranged above the pressing plate (4), the lower end of the cell culture box is inserted into the supply box (2) and is contacted with the panel (3), the width and the length of the cell culture box are matched with the length and the width inside the supply box (2), a liquid injection pipe (6) is vertically arranged at the center of the bottom surface inside the culture bottle (1), the upper end of the liquid injection pipe (6) is hermetically arranged, a sealing rod (7) is arranged inside the liquid injection pipe (6), a plurality of liquid outlet holes (18) are arranged on the outer side surface of the liquid injection pipe (6), the liquid outlet holes (18) are hermetically arranged by the sealing rod (7), liquid injection holes communicated with the liquid injection pipe are arranged on the bottom surface of the culture bottle (1), and through holes (11) are arranged right at the panel (3), the liquid supply pipe (12) is installed just to liquid injection hole department in clamp plate (4), and liquid supply pipe (12) upper end all seals the setting, all is equipped with a plurality of liquid feed mouths (13) on the outer wall of liquid supply pipe (12), just all is equipped with the liquid injection hole to liquid supply pipe department on the bottom surface of clamp plate (4), all forms sealed chamber (5) between clamp plate (4) and feed box (2).

2. The nutrient solution supply device for cell culture according to claim 1, characterized in that: all install rubber layer (19) on the outer wall face of sealing rod (7), rubber layer (19) all with annotate the internal face contact of liquid pipe (6), first spring (8) are all installed to sealing rod (7) upper end, and first spring (8) other end is all installed on annotating the inside top surface of liquid pipe (6).

3. The nutrient solution supply device for cell culture according to claim 1, characterized in that: all install the rubber frame on the side of clamp plate (4), the lateral surface of rubber frame touches with the internal face of feed box (2), polylith slider (14) are all installed to the both sides of clamp plate (4) and panel (3), the slider on clamp plate (4) all passes the rubber frame setting, the internal face of feed box (2) is just all being equipped with spout (21) to slider department, spout (21) two equal length two short settings, the equal slidable mounting of slider on panel (3) is in longer spout, the equal slidable mounting of slider on clamp plate (4) is in shorter spout, all install second spring (15) on the bottom surface of slider (14), the other end of second spring (15) is all installed on the bottom surface of spout (21), the cross-section of slider (14) and spout (21) all is the trapezium structure setting.

4. The nutrient solution supply device for cell culture according to claim 1, characterized in that: a liquid adding pipe (16) communicated with the sealing cavity is arranged on the outer side surface of the supply box (2), and the other end of the liquid adding pipe (16) is in threaded connection with a sealing cover (17).

5. The nutrient solution supply device for cell culture according to claim 1, characterized in that: the upper ends of the culture bottles (1) are provided with ventilating covers (9).

6. The nutrient solution supply device for cell culture according to claim 1, characterized in that: the culture bottle (1) on one side is provided with scales.

7. The nutrient solution supply device for cell culture according to claim 1, characterized in that: the supply box (2) is made of transparent medical plastic material.